123 results about "Single gene" patented technology

The invention provides a construction method of a predictive cell senescence model, a method for predicting the senescence state of a tissue sample based on the senescence model, a method for screening potential therapeutic drugs, equipment, a medium and a program product, and relates to the field of intelligent medical treatment. The model construction method comprises the following steps: acquiring a training set sample expression profile data set; identifying a key senescence gene set from the data set by using a feature selection algorithm; inputting the key senescence gene set into a machine learning model to fit a prediction model, and determining an optimal hyper-parameter to obtain a cell senescence model containing the weight of a single gene in the key senescence gene set; the cell senescence model is a senescence score obtained by calculating the sum of the product of the expression quantity of a single gene and the regression coefficient thereof. The cell senescence model, namely PreCSenM, is constructed by integrating a plurality of senescence characteristic gene sets and a gene scoring algorithm, the accuracy in CS evaluation is superior to that of 10 existing methods, and the application of CS from biological research to clinical scenes is also realized.

The application belongs to the field of intelligent medical treatment, and particularly relates to a method for constructing a virus single-gene host adaptability prediction model, equipment and a medium. The method comprises the following steps: obtaining a training set virus single-gene data set and a host label; encoding the single genes in the virus single-gene data set in sequence to obtain an encoding feature matrix set; the encoding is as follows: using a sliding window method to traverse a gene sequence, the frequency of 64 different codons in a window is used to form a window codon frequency vector, the window codon frequency vector is used to represent a central codon of the window, and each codon of the gene sequence is represented as a window codon frequency vector in sequence; inputting the encoding feature matrix set into a residual network model to obtain a prediction label, and iteratively training based on a comparison between the prediction label and the host label to obtain a single-gene host adaptability prediction model. The model capable of predicting gene adaptability is trained by using the representation of codons, and the analysis of gene codon adaptability can be realized.

In certain embodiments methods of treating X-Linked agammaglobulinemia (XLA) in a mammal are provided where the methods comprise: i) providing differentiated T cells and / or stem / progenitor cells from the mammal; ii) performing a targeted insertion of a corrective BTK cDNA at the BTK gene locus in said cells to provide a corrected BTK gene in said cells; and iii) introducing said cells into said mammal where said corrected BTK gene is expressed in a physiologically regulated manner.

The present invention discloses a primer probe set and a kit for fluorescent quantitative PCR for detecting influenza D virus and its lineages. The primer probe set consists of a group of primer probes IDV PB-F, IDV PB-R, IDV PB-Prob for detecting influenza D virus, and a group of primer probes IDV HEF-F, IDV HEF-R, IDV HEF-Prob for identifying lineages. The primer probe set can realize differential diagnosis of five lineage strains of IDV in one reaction, which can not only confirm whether the influenza D virus is infected, but also reveal which lineage the detected strain belongs to, and is more efficient and accurate than single gene detection. The kit developed based on this has a good application prospect, and truly realizes a fully enclosed and pollution-free integrated reaction from "sample in" to "result out".

The application provides a personalized cancer driver gene identification method based on network propagation, and the implementation steps are as follows: obtaining related data of cancer driver gene identification; performing data fusion on abnormal genomics data; constructing a cancer gene regulation network of a group; constructing a personalized cancer gene regulation network; calculating influence score of a node pair; and obtaining a personalized cancer driver gene identification result through a network propagation process. In the process of constructing the personalized cancer gene regulation network, statistical analysis is performed on the interaction between each pair of genes of each patient, so that the deviation of the network model caused by abnormal expression of a single gene in the prior art is avoided; and in the process of network propagation, various genomics data and topological information in the network are integrated, the information abundance in the network model is improved, and the accuracy of identifying personalized driver genes is effectively improved.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

The invention relates to a full-length sequence amplification primer, an amplification method and a three-generation sequencing method of an HLA-I antigen gene, and belongs to the field of gene detection. The HLA-I antigen gene is subjected to PCR (Polymerase Chain Reaction) amplification by using 8 pairs of characteristic amplification primers. And each gene can be subjected to high-yield specific amplification on a full-length sequence containing complete intron and exon regions only through one round of amplification and enrichment, and 7.8 Kb long-fragment genes can be captured once at most. The experimental deviation caused by simultaneous amplification of a single gene by using a plurality of pairs of segmented primers and the indirect error caused by gene splicing are effectively avoided. The method comprises the following steps: carrying out quality inspection and purification on amplicon PCR (Polymerase Chain Reaction) products, mixing samples, constructing an HLA-I type antigen gene library, carrying out accurate and complete sequencing on the full-length sequence of the HLA-I type antigen gene by adopting a three-generation PacBio Sequel II platform, and ensuring that the Hifi reads base accuracy can reach 99% or above. The reading of HLA-I type antigen gene full-length sequence variation information and the haplotype analysis at a high resolution level can be realized.

The present application relates to the field of plant biotechnology, and in particular to a method for enhancing the nicotine synthesis level of tobacco, which comprises simultaneously overexpressing arginine decarboxylase gene NtADC1 (SEQ ID NO: 13) and transcription factor gene NtMYC2 (SEQ ID NO: 14), transforming tobacco, and obtaining a co-expression plant through hybridization. Experiments show that the nicotine content of the co-expression plant after topping is 310% of the wild type, which is significantly higher than that of the single gene overexpression plant (NtMYC2 overexpression increases by 35%, and NtADC1 overexpression increases by 5%). The method solves the technical bottleneck of the prior art that the nicotine improvement range is less than 40%, and provides a new scheme for high nicotine tobacco breeding.

This invention belongs to the field of genetic engineering and cell modification technology, and discloses a method for constructing and applying anti-aging human mesenchymal stem cells (MSCs). The DHX9-overexpressing virus-transfected MSCs constructed in this invention possess stable DHX9 overexpression effects and anti-aging functions. These MSCs do not target a single gene locus, but rather alleviate a series of aging phenotypes and genomic instability issues during the aging process by eliminating R-loops. DHX9-overexpressing MSCs exhibit significant anti-aging activity, as well as strong environmental adaptability and excellent safety characteristics. They can effectively resist harsh in vivo inflammation and the aging microenvironment, while avoiding the tumorigenic risk after cell transplantation. These MSCs with stable anti-aging capabilities can subsequently be used to significantly improve the efficacy of MSC infusion therapy for diseases.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

The invention provides modRNA (Ribonucleic Acid) for treating obliterated bronchitis, and belongs to the technical field of bioengineering. The modRNA for treating obliterated bronchitis provided by the invention is composed of an IL-10 modRNA (Interleukin-10) and an IFN (Interferon)-alpha modRNA (Interferon-alpha) modRNA. The BO treatment effect is achieved by inhibiting bronchial epithelial cell epithelial-mesenchymal transition (EMT), the expression of alpha-SMA and Vimentin in lung tissue can be remarkably reduced, the expression of E-cadherin and Cytokeratin 5 can be up-regulated, microbronchial epithelial injury is repaired, collagen deposition is reduced, and the lung function is improved. The synergistic effect of the two is better than that of single gene therapy, the safety is high, and the controllability is strong.

The application belongs to the technical field of molecular biology and medicine, and discloses a methylation marker for detecting the benignity and malignancy of thyroid nodules and an application. 780 new methylation sites are identified, 273 tumor occurrence and development related genes are associated, and the Fc gamma R-mediated phagocytosis signal pathway is involved. The methylation marker for detecting the benignity and malignancy of thyroid nodules comprises multiple genes and multiple methylation sites. The methylation level of multiple genes and multiple sites is detected, the problem of low and unstable methylation signal of single gene or single site is overcome, and thus the sensitivity and specificity of detection are improved.

The application discloses a kind of screening methods and application of pivot gene related to sheep muscle fatty acid content, to solve the technical problems that local sheep breed sample quantity is limited, traditional single gene analysis method is difficult to analyze fatty acid metabolism regulation from network level.This application carries out transcriptome sequencing to multiple months of muscle tissue of Gangba sheep, constructs gene expression matrix, using weighted gene co-expression network analysis (WGCNA) Combined with module characteristic gene and fatty acid phenotype correlation screening strategy, the pivot gene significantly positively correlated with muscle fatty acid content is obtained.The screening method can construct a robust co-expression network under limited sample size, systematically identify the functional module and core gene related to the content of fatty acids such as linoleic acid, and reveal the dynamics of fatty acid metabolism at different ages, and the method can be extended to other plateau livestock;The screened pivot gene can be used as a molecular breeding marker for early selection of Gangba sheep, and the breeding cycle is shortened.

The invention discloses an identification method, device and system for a high gene module scoring area, and relates to the technical field of space transcriptomics. The identification method comprises the following steps: acquiring space transcriptome data, dividing cells into a plurality of unit grids according to space coordinate information of the cells, and determining corresponding grid space coordinates for each unit grid; calculating a module score value of each cell and determining a high-score cell set; calculating an integrated gene set scoring index; and carrying out region division on the unit grid and determining a high gene module scoring region. According to the method, a plurality of genes are analyzed as a functional whole, the limitation that traditional single-gene analysis cannot reveal collaborative regulation is overcome, spatial quantization of a gene set synergistic effect is realized, and the problem that a gene set enrichment region cannot be positioned in the prior art is solved.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

The invention discloses a GPC1 gene and application thereof, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the GPC1 gene is shown as SEQ ID NO.2. The increase of the single gene expression quantity of the CPC1 gene leads to the significant increase of the whole grain protein content of the rice, but the knockout of the gene leads to the significant reduction of the whole grain protein content of the rice, and the whole grain protein content of the rice in a near-isogenic line of the allele has significant difference. The gene is used for improving the characters of rice varieties, has huge application potential and prospect, and provides a new gene resource for providing rice nutritional quality and cooking quality breeding.

The invention discloses a salmonella low water activity tolerance prediction method, device and system and a storage medium, and the method comprises the steps: collecting salmonella genome data in a public database, and recording the water activity information of a source; processing the genome data to form a data matrix; performing redundancy elimination operation on the data matrix; dividing the data matrix; respectively carrying out model training through three machine learning algorithms by using the data matrix; obtaining an optimal performance model based on a Bayesian parameter adjustment method; and the low water activity tolerance of the salmonella is judged based on prediction of three algorithm models. By adopting the technical scheme provided by the invention, the problems of insufficient prediction coverage caused by dependence on a single gene marker and overfitting caused by high whole genome feature redundancy in the prior art are solved.

The invention discloses a multiplex PCR (polymerase chain reaction) method for quickly detecting pathogenic bacteria of tobacco brown spot, which is used for synchronously identifying two main pathogenic bacteria causing tobacco brown spot, namely alternaria tenuissima and alternaria alternata, and comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid): extracting genome DNA of standard strains of alternaria tenuissima and alternaria alternata as a detection template; the multiple PCR system has the beneficial effects that through system verification, it is confirmed that all primers only generate expected amplification products for target pathogenic bacteria, no cross amplification reaction exists between alternaria alternata and alternaria tenuissima, and it is indicated that the multiple PCR system has high interspecific specificity. The established detection method is high in stability, good in repeatability and suitable for standardized operation under different laboratory conditions. Compared with a traditional morphological identification or single gene sequencing method, the multiplex PCR technology has the advantages that the detection period is remarkably shortened, the operation is simple and convenient, time and labor are saved, the cost is low and the like. The method is suitable for rapid identification of standard strains.

The invention discloses a Brucella bacterium capable of generating a lipoid A vaccine adjuvant and an application of the Brucella bacterium. According to the invention, recombinant plasmids for expressing BacA and LpxL genes are constructed, and are transformed into Brucella through electric shock, so that a double-gene knockout strain is successfully obtained. By knocking out BacA, an ultra-long fatty acid chain of lipoid A is shortened, an organism is induced to generate an inflammatory reaction, and by knocking out LpxL, the inflammatory reaction is reduced, so that the effect of the immunologic adjuvant is achieved. LPS is extracted for in-vitro stimulation analysis, and it is found that the inflammatory response of double-gene deletion is reduced compared with that of single-gene deletion. Animal experiments show that double-gene deletion can induce an organism to generate good immune response and induce relatively high cellular immunity and humoral immunity, and a new thought is provided for research and development of gram-negative bacterium inactivated vaccines.

The application discloses a method for analyzing B lymphocyte BCR characteristics based on single-cell multi-omics sequencing. The method collects samples of patients in different disease states for single-cell sequencing, constructs a single-cell gene expression matrix based on single-cell transcriptome sequencing data and carries out cell type annotation, assembles BCR sequences based on single-cell immunome sequencing data and identifies BCR chain composition genes, then evaluates and identifies the stable BCR expression rate of each cell subpopulation, evaluates the clonal state of each cell subpopulation in different disease states, analyzes the BCR assembly and single-gene use preference of each sample in different disease states, and the BCR assembly and V-J gene pair use preference. The application has important significance for the mechanism analysis of specific viral infection hosts and the research and development of specific vaccines and drugs.

Disclosed are methods for preparing a non-naturally occurring composition, comprising: extracting cell-free DNA from a plasma fraction of a blood sample of a pregnant person, wherein the pregnant person is a heterozygous carrier of at least one pathogenic variant in at least one target gene associated with autosomal recessive disorders, wherein the extracted cell-free DNA comprises a mixture of maternal cell-free DNA and fetal cell-free DNA; performing targeted enrichment on the extracted cell-free DNA or DNA derived therefrom to enrich a plurality of target variant loci in a plurality of target genes and generating enriched DNA, wherein the target variant loci comprise the at least one pathogenic variant; performing high-throughput sequencing on the enriched DNA or DNA derived thereof and generating sequence reads, and determining fetal genotypes of one or more of the target genes from the sequence reads.

The application belongs to modern agriculture, and particularly relates to TIFY8 Application of the gene and the protein in salt tolerance of rice seedlings. The application screens the knockout mutant material with salt stress tolerance by subjecting the single gene knockout mutant material library constructed in the laboratory to salt stress treatment. After salt treatment and identification, tify8 The knockout mutant has higher salt stress tolerance and survival rate than the wild type. Therefore, the application aims to cultivate the salt tolerance line of rice by using TIFY8 the functional characteristics of the gene and tify8 the knockout mutant system. The application can significantly shorten the breeding period compared with the traditional breeding method.

The invention belongs to the technical field of gene engineering and cell transformation, and discloses a gene engineering construction method and application of anti-aging human mesenchymal stem cells. The DHX9 overexpression virus transfected MSCs constructed by the invention have a stable DHX9 overexpression effect and an anti-aging function, and the MSCs relieve a series of problems of aging phenotypes and unstable genomes in an aging process by eliminating R-loop instead of aiming at a single gene locus. The DHX9 overexpression MSCs show significant anti-aging activity, also have strong adaptive capacity to environment and excellent safety characteristics, can effectively resist harsh in-vivo inflammation and aging microenvironment, and at the same time avoids tumorigenic risk after cell transplantation. The MSCs with the stable anti-aging capability can be subsequently applied to remarkably improving the treatment effect of MSCs infused into a body for treating diseases.

The utility model relates to a single-gene genetic disease detection and screening device which comprises a tube body and a tube cover which cover each other, and the tube body is in threaded connection with the tube cover; the tube body is used for accommodating a sample preservation solution, and a breaking groove is formed in the side wall of the tube cover and is used for accommodating a swab; when the groove bottom wall of the breaking groove is used for abutting against the breaking point of the swab, the tube body is inserted into the tube cover. In the application, the groove is broken from the side wall of the tube cover, so that the tube cover and the tube body clamp the swab, and the swab is broken; part of the tube body is inserted into the tube cover, so that a user can conveniently hold the tube body and the tube body. When the swab is broken off by a user, the risk that the tube body, the tube cover and the swab are separated from each other can be reduced, so that a childbearing-age couple can sample saliva conveniently, and detection and screening of single-gene genetic diseases are carried out.

The invention discloses a scoring and sorting method and system for pathogenic mutation of a single-gene genetic disease, and relates to the technical field of biomedical treatment, the method comprises the following steps: based on pre-acquired phenotypic data and literature abstracts, performing standardized phenotypic term extraction on pre-acquired symptom description by using a mixed strategy, performing phenotype and gene association degree scoring on the standardized phenotype terms and pre-acquired gene mutation data through a similarity comparison method; performing gene variation annotation on a pre-acquired gene variation database, and performing gene variation scoring on the pre-acquired gene variation data according to a gene variation annotation result; and performing multi-dimensional pathogenicity comprehensive scoring evaluation by using a supervised learning model and a weighted summation method to obtain a pathogenic mutation sorting scoring result. By recommending the most probable pathogenic mutation, the grading and sorting of the pathogenic mutation of the genetic disease have the advantages of high detection recall rate and high automation degree.

The invention provides a multiplex PCR (polymerase chain reaction) primer combination and a sequencing analysis method for identifying a species of Schizothorax mali, and belongs to the technical field of molecular detection. According to the multiple PCR primer combination disclosed by the invention, a plurality of primer pairs are designed according to multiple key gene SNP locus areas of mitochondrial genomes (imagoes, larvae and pupae) of different forms and different populations and sibling species of the mitochondrial genomes, so that a plurality of target fragments can be synchronously amplified by single PCR reaction; the misjudgment caused by single gene variation or sequencing errors is effectively avoided, and the sibling species and the cryptic species with highly similar forms can be accurately distinguished. The invention further provides a multiple PCR and sequencing analysis method for identifying the species of the malodinia mali, targeted high-throughput sequencing is conducted on multiple PCR amplification products, sequence information of all key sites is obtained, the similarity of the sites to be detected can be obtained through comparison, and decisive data support is provided for high-throughput accurate identification of the species level of the malodinia mali.