67 results about "Single gene" patented technology

The application provides a personalized cancer driver gene identification method based on network propagation, and the implementation steps are as follows: obtaining related data of cancer driver gene identification; performing data fusion on abnormal genomics data; constructing a cancer gene regulation network of a group; constructing a personalized cancer gene regulation network; calculating influence score of a node pair; and obtaining a personalized cancer driver gene identification result through a network propagation process. In the process of constructing the personalized cancer gene regulation network, statistical analysis is performed on the interaction between each pair of genes of each patient, so that the deviation of the network model caused by abnormal expression of a single gene in the prior art is avoided; and in the process of network propagation, various genomics data and topological information in the network are integrated, the information abundance in the network model is improved, and the accuracy of identifying personalized driver genes is effectively improved.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

The invention relates to a full-length sequence amplification primer, an amplification method and a three-generation sequencing method of an HLA-I antigen gene, and belongs to the field of gene detection. The HLA-I antigen gene is subjected to PCR (Polymerase Chain Reaction) amplification by using 8 pairs of characteristic amplification primers. And each gene can be subjected to high-yield specific amplification on a full-length sequence containing complete intron and exon regions only through one round of amplification and enrichment, and 7.8 Kb long-fragment genes can be captured once at most. The experimental deviation caused by simultaneous amplification of a single gene by using a plurality of pairs of segmented primers and the indirect error caused by gene splicing are effectively avoided. The method comprises the following steps: carrying out quality inspection and purification on amplicon PCR (Polymerase Chain Reaction) products, mixing samples, constructing an HLA-I type antigen gene library, carrying out accurate and complete sequencing on the full-length sequence of the HLA-I type antigen gene by adopting a three-generation PacBio Sequel II platform, and ensuring that the Hifi reads base accuracy can reach 99% or above. The reading of HLA-I type antigen gene full-length sequence variation information and the haplotype analysis at a high resolution level can be realized.

The present application relates to the field of plant biotechnology, and in particular to a method for enhancing the nicotine synthesis level of tobacco, which comprises simultaneously overexpressing arginine decarboxylase gene NtADC1 (SEQ ID NO: 13) and transcription factor gene NtMYC2 (SEQ ID NO: 14), transforming tobacco, and obtaining a co-expression plant through hybridization. Experiments show that the nicotine content of the co-expression plant after topping is 310% of the wild type, which is significantly higher than that of the single gene overexpression plant (NtMYC2 overexpression increases by 35%, and NtADC1 overexpression increases by 5%). The method solves the technical bottleneck of the prior art that the nicotine improvement range is less than 40%, and provides a new scheme for high nicotine tobacco breeding.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

The invention provides modRNA (Ribonucleic Acid) for treating obliterated bronchitis, and belongs to the technical field of bioengineering. The modRNA for treating obliterated bronchitis provided by the invention is composed of an IL-10 modRNA (Interleukin-10) and an IFN (Interferon)-alpha modRNA (Interferon-alpha) modRNA. The BO treatment effect is achieved by inhibiting bronchial epithelial cell epithelial-mesenchymal transition (EMT), the expression of alpha-SMA and Vimentin in lung tissue can be remarkably reduced, the expression of E-cadherin and Cytokeratin 5 can be up-regulated, microbronchial epithelial injury is repaired, collagen deposition is reduced, and the lung function is improved. The synergistic effect of the two is better than that of single gene therapy, the safety is high, and the controllability is strong.

The application belongs to the technical field of molecular biology and medicine, and discloses a methylation marker for detecting the benignity and malignancy of thyroid nodules and an application. 780 new methylation sites are identified, 273 tumor occurrence and development related genes are associated, and the Fc gamma R-mediated phagocytosis signal pathway is involved. The methylation marker for detecting the benignity and malignancy of thyroid nodules comprises multiple genes and multiple methylation sites. The methylation level of multiple genes and multiple sites is detected, the problem of low and unstable methylation signal of single gene or single site is overcome, and thus the sensitivity and specificity of detection are improved.

The application discloses a kind of screening methods and application of pivot gene related to sheep muscle fatty acid content, to solve the technical problems that local sheep breed sample quantity is limited, traditional single gene analysis method is difficult to analyze fatty acid metabolism regulation from network level.This application carries out transcriptome sequencing to multiple months of muscle tissue of Gangba sheep, constructs gene expression matrix, using weighted gene co-expression network analysis (WGCNA) Combined with module characteristic gene and fatty acid phenotype correlation screening strategy, the pivot gene significantly positively correlated with muscle fatty acid content is obtained.The screening method can construct a robust co-expression network under limited sample size, systematically identify the functional module and core gene related to the content of fatty acids such as linoleic acid, and reveal the dynamics of fatty acid metabolism at different ages, and the method can be extended to other plateau livestock;The screened pivot gene can be used as a molecular breeding marker for early selection of Gangba sheep, and the breeding cycle is shortened.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

The invention discloses a GPC1 gene and application thereof, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the GPC1 gene is shown as SEQ ID NO.2. The increase of the single gene expression quantity of the CPC1 gene leads to the significant increase of the whole grain protein content of the rice, but the knockout of the gene leads to the significant reduction of the whole grain protein content of the rice, and the whole grain protein content of the rice in a near-isogenic line of the allele has significant difference. The gene is used for improving the characters of rice varieties, has huge application potential and prospect, and provides a new gene resource for providing rice nutritional quality and cooking quality breeding.

The invention discloses a salmonella low water activity tolerance prediction method, device and system and a storage medium, and the method comprises the steps: collecting salmonella genome data in a public database, and recording the water activity information of a source; processing the genome data to form a data matrix; performing redundancy elimination operation on the data matrix; dividing the data matrix; respectively carrying out model training through three machine learning algorithms by using the data matrix; obtaining an optimal performance model based on a Bayesian parameter adjustment method; and the low water activity tolerance of the salmonella is judged based on prediction of three algorithm models. By adopting the technical scheme provided by the invention, the problems of insufficient prediction coverage caused by dependence on a single gene marker and overfitting caused by high whole genome feature redundancy in the prior art are solved.

The invention discloses a multiplex PCR (polymerase chain reaction) method for quickly detecting pathogenic bacteria of tobacco brown spot, which is used for synchronously identifying two main pathogenic bacteria causing tobacco brown spot, namely alternaria tenuissima and alternaria alternata, and comprises the following steps: S1, extracting genome DNA (deoxyribonucleic acid): extracting genome DNA of standard strains of alternaria tenuissima and alternaria alternata as a detection template; the multiple PCR system has the beneficial effects that through system verification, it is confirmed that all primers only generate expected amplification products for target pathogenic bacteria, no cross amplification reaction exists between alternaria alternata and alternaria tenuissima, and it is indicated that the multiple PCR system has high interspecific specificity. The established detection method is high in stability, good in repeatability and suitable for standardized operation under different laboratory conditions. Compared with a traditional morphological identification or single gene sequencing method, the multiplex PCR technology has the advantages that the detection period is remarkably shortened, the operation is simple and convenient, time and labor are saved, the cost is low and the like. The method is suitable for rapid identification of standard strains.

Disclosed are methods for preparing a non-naturally occurring composition, comprising: extracting cell-free DNA from a plasma fraction of a blood sample of a pregnant person, wherein the pregnant person is a heterozygous carrier of at least one pathogenic variant in at least one target gene associated with autosomal recessive disorders, wherein the extracted cell-free DNA comprises a mixture of maternal cell-free DNA and fetal cell-free DNA; performing targeted enrichment on the extracted cell-free DNA or DNA derived therefrom to enrich a plurality of target variant loci in a plurality of target genes and generating enriched DNA, wherein the target variant loci comprise the at least one pathogenic variant; performing high-throughput sequencing on the enriched DNA or DNA derived thereof and generating sequence reads, and determining fetal genotypes of one or more of the target genes from the sequence reads.

The invention provides a multiplex PCR (polymerase chain reaction) primer combination and a sequencing analysis method for identifying a species of Schizothorax mali, and belongs to the technical field of molecular detection. According to the multiple PCR primer combination disclosed by the invention, a plurality of primer pairs are designed according to multiple key gene SNP locus areas of mitochondrial genomes (imagoes, larvae and pupae) of different forms and different populations and sibling species of the mitochondrial genomes, so that a plurality of target fragments can be synchronously amplified by single PCR reaction; the misjudgment caused by single gene variation or sequencing errors is effectively avoided, and the sibling species and the cryptic species with highly similar forms can be accurately distinguished. The invention further provides a multiple PCR and sequencing analysis method for identifying the species of the malodinia mali, targeted high-throughput sequencing is conducted on multiple PCR amplification products, sequence information of all key sites is obtained, the similarity of the sites to be detected can be obtained through comparison, and decisive data support is provided for high-throughput accurate identification of the species level of the malodinia mali.

The application discloses a Toxoplasma SRS29C and ROP16 double-gene deletion strain and a construction method and application thereof, relates to the technical field of parasite genetic engineering and molecular biology. The Toxoplasma SRS29C and ROP16 double-gene deletion strain is obtained by knocking out SRS29C and ROP16 genes of the ME49 strain through a CRISPR / Cas9 system. The double-gene deletion strain constructed by the application has drug sensitivity change (sensitivity to ethidium bromide), growth defect phenotype, and only carries a single screening marker, and under in-vitro culture conditions, the double-gene deletion strain presents a significant slow growth trend, and the number of worm-containing bubbles is obviously less than that of the wild type and single-gene deletion strain. The double-gene deletion strain substantially influences the proliferation of the worm body, and provides key data support for exploring the molecular basis of the double-gene deletion leading to the increase of the drug sensitivity of the worm body and for analyzing the regulation mechanism of the drug tolerance related path of the Toxoplasma.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica with high content of Cys, Met and His as well as a construction method and application of the yarrowia lipolytica. Specifically, the yarrowia lipolytica strain with high Cys, Met and His contents is successfully constructed through a genetic engineering means, the comprehensive content of three target amino acids accounts for 3.0% or above of the dry cell weight, meanwhile, the construction method of the strain is simple and efficient, the content of the target amino acids can be remarkably increased through overexpression of a single gene, and industrial popularization is easy. The total protein content of the strain is kept at a higher level of 40%-65%, and the prepared single-cell protein is excellent in nutritional value and wide in application prospect by combining with rich target amino acids.

This invention provides GhFRC1 The application of genes in regulating plant fertility belongs to the field of plant genetic engineering technology. This invention is the first to clone genes from cotton. GhFRC1 The gene was identified and verified to be heterologously expressed in three different genera and families of crops: Arabidopsis thaliana, tomato, and rice, and to play a role in fertility regulation. This breakthrough overcomes the limitation of existing fertility genes that can only regulate the fertility of a single crop, and realizes "broad-spectrum regulation of fertility of multiple crops by a single gene", filling the gap in the application of distant heterologous expression of cotton fertility genes. GhFRC1 After heterologous expression of the gene, it can stably lead to inactive pollen and abnormal fruit set in the recipient crop, and can also cause a phenotype of complete pollen sac non-development. Moreover, the sterility trait can be restored to fertility through normal pollen pollination. This solves the problem of the narrow applicability of existing fertility genes and the great limitation of their breeding applications, and provides new gene resources and technical solutions for fertility regulation of multiple crops and hybridization breeding.

The application discloses a biomarker mining model training method and device and related equipment, comprising: providing a number of biological sample corresponding transcriptome original data and sample phenotype category label, obtaining a gene expression matrix from the transcriptome original data, processing the matrix to obtain a high-dimensional gene expression feature vector, inputting the high-dimensional gene expression feature vector corresponding to each biological sample into a biomarker mining model, the biomarker mining model obtains the confidence value of each sample phenotype category based on the high-dimensional gene expression feature vector, and predicts the sample phenotype category based on the confidence value. The application extracts features from transcriptome data, uses the feature vector and the corresponding sample phenotype category label as training data for training, sets a confidence calculation in the trained model, realizes quantitative evaluation of the importance of gene features, determines the marginal influence of single gene feature change on the prediction result, and thus determines the biomarker.

The application provides poplar PagTAL3 protein and an application of a coding gene thereof, relates to the technical field of genetic engineering, and the amino acid sequence of the PagTAL3 protein is shown in SEQ ID NO. 1. The single-gene expression quantity of the PagTAL3 protein is enhanced, so that the synthesis of melatonin, the chloroplast photosynthesis, the synthesis of cell wall components and the transaldolase activity of poplar are enhanced; and the gene editing mutant is reduced in the synthesis of melatonin, the chloroplast photosynthesis, the synthesis of cell wall components and the transaldolase activity. The gene has great application potential and prospect for improving the poplar variety traits.

The invention discloses a multiple PCR (Polymerase Chain Reaction) Panel for detecting favorable allelic variation of wheat yield related traits and application of the multiple PCR Panel. The multiplex PCR Panel takes 9 genetic synergistic sites related to wheat yield formation as targets and is used for performing targeted detection on corresponding favorable allelic variation. The nucleotide sequences of the primer group are as shown in SEQ ID No. 21 to SEQ ID No. 38. The PCR Panel can simultaneously and accurately analyze excellent allelic variation of a grain weight synergistic site, a grain width synergistic site and a plant height related site in a single reaction, and compared with a traditional marker detection mode taking a single gene or a single character as a target, the PCR Panel has the advantage that the analysis efficiency of multi-site genetic information can be remarkably improved. The multiple PCR Panel system is embedded into a conventional wheat breeding process, a cooperative breeding mode of'precise complementary matching of parents, primary screening of low-generation basic agriculture and targeted molecular selection of middle-generation and high-generation 'is constructed, rapid selection, polymerization and fixation of multiple sites of yield-related traits are realized, the breeding period is shortened, and the new germplasm creation efficiency is improved.

The invention relates to the technical field of biological medicines, and particularly discloses a cancer suppression small interfering RNA (Ribonucleic Acid) capable of simultaneously targeting cancer genes PTTG1 and STMN1 and application of the cancer suppression small interfering RNA, and the technical key points are as follows: the positive-sense strand sequence of the small interfering RNA is GGGAGAUCUCAAGUUUCAATT, and the antisense strand sequence of the small interfering RNA is UUGAAACUUGAGAUCUCCCTT. The small interfering RNA provided by the invention can specifically silence the expression of PTTG1 and STMN1 at the same time. Compared with siRNA (such as siRNA for ASCC3 or TRAPPC4) targeting a single gene in the prior art, the siRNA provided by the invention has the advantages that synergistic inhibition on tumor proliferation and metastasis pathways is realized through double-targeting design, and the inhibition effect on tumor cell growth is more remarkable; compared with a method of physically mixing two single siRNAs, the method provided by the invention has higher cell apoptosis induction efficiency and better in-vivo tumor inhibition effect; the small interfering RNA is effective in various liver cancer cells, has a lasting effect in an animal model, and provides a core molecular entity for developing novel antitumor drugs.

The invention relates to the technical field of cell separation and purification and cell biological detection, in particular to a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman. The invention provides a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman, which is characterized in that a LukSF and LukAB bi-component perforation toxin is designed and utilized to specifically recognize and split a CXCR1 / 2 chemotactic factor receptor and a CD11b / CD18 integrin receptor on the surface of maternal leukocyte, and the fetal NRBC is completely reserved due to lack of corresponding receptors, so that the fetal NRBC can be separated and purified. Therefore, leukocyte pollution in nucleated red blood cells of a peripheral blood fetus of a pregnant woman is efficiently removed. According to the method, the purity of the extracted fetal NRBC can be improved, the false positive of gene detection is greatly reduced, the activity and integrity of the NRBC are reserved, and the accurate diagnosis requirements of chromosome karyotype analysis, monogenic disease detection and the like are met; the method is simple to operate and low in cost, can be directly adapted to existing clinical laboratory equipment, and has large-scale popularization value.

The invention provides a PagTAL3 protein of a poplar and application of a coding gene of the PagTAL3 protein, and relates to the technical field of gene engineering, and the amino acid sequence of the PagTAL3 protein is as shown in SEQ ID NO.1. The enhancement of the single gene expression quantity causes the enhancement of poplar melatonin synthesis, chloroplast photosynthesis, cell wall component synthesis and transaldolase activity; the gene editing mutant is characterized by melatonin synthesis, chloroplast photosynthesis, cell wall component synthesis and transaldolase activity reduction. The gene has huge application potential and prospect in improvement of poplar variety characters.

This invention discloses an InDel marker closely linked to powdery mildew resistance in wild bitter gourd and its application. This invention obtains highly resistant wild germplasm resources to powdery mildew through screening, with resistance controlled by a dominant single gene. Through forward genetic mapping, a molecular marker PM869 closely linked to the resistance gene was successfully developed. Using the PM869 marker of this invention to identify powdery mildew resistance in bitter gourd is characterized by its simplicity, high specificity, and high stability. It can accurately and efficiently detect the resistance and susceptibility of tested bitter gourd plants to powdery mildew, with a 99% concordance rate with field results. Using this method to assist in the identification of powdery mildew resistance in bitter gourd allows for the screening of resistant individual plants at the seedling stage, enabling the screening of large batches of samples, greatly shortening the bitter gourd breeding cycle and improving breeding efficiency.

Soybean plants, plant parts and plant cells capable of producing seeds comprising oil having relatively higher oleic acid levels and lower linoleic and linolenic acid levels than corresponding seeds lacking the targeted mutation.SOLUTION: Disclosed are TALEN compositions and methods of use, including using a multiplexing composition to make targeted mutations in several genes at once, such as the FAD3A / B / C genes, compositions for making targeted mutations in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide genetically edited plants, plant parts, and plant cells with improved characteristics as compared to corresponding unmodified plants, plant parts, or plant cells.SELECTED DRAWING: FIG. 1A

The present disclosure relates to a device and method for predicting the risk of disease occurrence by utilizing single nucleotide polymorphisms and the presence or absence of monogenic variants in a subject. According to the device and method according to an aspect, prediction of the risk of disease occurrence is enabled based on a more accurate genetic risk by integrating together monogenic variants, which are based on the subject's genetic information and have a clear causal relationship but appear rarely, and the polygenic risk score, which is based on commonly occurring single nucleotide polymorphisms that, in comparison, do not have high individual association.

The invention relates to the technical field of molecule and cell biology, and discloses a preparation method of a polygene methylation detection kit based on fecal DNA, which comprises the following steps: preparing a specific primer and probe mixture: the mixture comprises specific primers and probes for detecting methylation states of BMP3, NDRG4, TFPI2, SDC2 and SFRP2 genes, and internal reference primers and probes for detecting ACTB genes; according to the invention, five genes of BMP3, NDRG4, TFPI2, SDC2 and SFRP2 are adopted for joint detection. The multi-gene combination strategy can effectively overcome the limitation that a single gene methylation signal is deleted or unstable in part of early cancer or precancerous lesions, and the detection rate (sensitivity) of colorectal cancer, especially early colorectal cancer, is greatly improved through multi-target and multi-channel coverage.

Sickle cell disease (SCD) is a single gene disorder characterized by mutant hemoglobin-S (HbS) and chronic intravascular haemolysis. Painful vaso-occlusive crises (VOC) are typical of SCD and often associated to a further rise in hemolysis. VOC is the clinically painful form of vaso-occlusion, that is due to the aggregation of red blood cells in the capillaries and venules. Such event is promoted or aggravated by adhesion of polymorphonuclear neutrophils (PMNs) to red blood cells and the endothelium leading to tissue ischemia, inflammation and imperfect repair. Repeated vaso-occlusion and PMNs interactions with the vascular endothelium are thought to promote microvascular injuries in SCD patients. The inventors tested the effect of pharmacological inhibition of TREM-1 with LR12 peptide in two experimental vaso-occlusive crisis models. Additional validation of TREM-1 involvement in vaso-occlusion was verified using mice with sickle cell disease and Trem-1 gene deficiency. In particular, the inventors showed that TREM-1 inhibition is particular suitable for limiting the severity of vaso-occlusions. The results obtained by the inventors also suggest that plasmatic concentration of sTREM-1 could be a reliable biomarker for predicting vaso-occlusions and / or SCD-associated organ dysfunction and end-organ damage.