156 results about "Single gene" patented technology

The invention provides a construction method of a predictive cell senescence model, a method for predicting the senescence state of a tissue sample based on the senescence model, a method for screening potential therapeutic drugs, equipment, a medium and a program product, and relates to the field of intelligent medical treatment. The model construction method comprises the following steps: acquiring a training set sample expression profile data set; identifying a key senescence gene set from the data set by using a feature selection algorithm; inputting the key senescence gene set into a machine learning model to fit a prediction model, and determining an optimal hyper-parameter to obtain a cell senescence model containing the weight of a single gene in the key senescence gene set; the cell senescence model is a senescence score obtained by calculating the sum of the product of the expression quantity of a single gene and the regression coefficient thereof. The cell senescence model, namely PreCSenM, is constructed by integrating a plurality of senescence characteristic gene sets and a gene scoring algorithm, the accuracy in CS evaluation is superior to that of 10 existing methods, and the application of CS from biological research to clinical scenes is also realized.

A method of treating or inhibiting a viral infection in a human subject, such as a SARS-CoV-2 infection, involves inhibiting in vivo the expression or activity of one or a combination of the subject's genes required for viral infection. Single genes or subsets of genes for inhibition of activity or expression are selected from certain identified genes. Methods of administration of certain known small molecules or other therapeutics which mimic loss of function of the identified genes are provided. Similar methods for conducting screens of host genes required for viral infection are shown.

The invention provides a multi-scale causal discovery method and system based on collective behavior modeling, and relates to the technical field of causal discovery, and the method comprises the steps: obtaining single-cell RNA sequencing data, the single-cell RNA sequencing data being a gene expression matrix of a plurality of cells; single-cell RNA sequencing data is input into a multi-scale causal model for reasoning, a multi-scale causal diagram is obtained, and the multi-scale causal diagram is used for describing the multi-scale causal relationship between genes and between genes and gene programs; wherein the multi-scale causal model takes a single gene as an individual variable, through collective behavior modeling, the single gene is aggregated into a collective variable representing a gene program, and a multi-scale collaborative learning strategy is adopted to collaboratively learn the multi-scale causal relationship between individuals and between individuals and collective. According to the method, a collective behavior modeling framework is innovatively constructed, key collective behaviors driving state changes can be effectively identified, and the causal discovery accuracy, rationality and robustness are improved.

The invention discloses dsRNA / fused dsRNA for RNAi targeting multiple aphids and application of the dsRNA / fused dsRNA, and the dsRNA targets an aphid Actin gene and / or an ELAV-like protein gene and is obtained by transcription by taking the aphid Actin gene and / or the ELAV-like protein gene as a template. Compared with the traditional design thought that dsRNA of pests mostly aims at one pest and is targeted to a single gene at present, the strategy is changed, starting from the thought that one dsRNA can prevent and treat various aphids, highly conservative genes of various aphids are found, the dsRNA is designed for the highly conservative genes, and it is found that in a plurality of candidate genes, various aphids can be effectively prevented and treated. The dsRNA aiming at the actin Actin gene and the ELAV-like protein gene has a remarkable fatality rate on various aphids and has a very good application prospect, the effect of simultaneously targeting the fused dsRNA of the two genes is further improved, and the dsRNA has a remarkable insecticidal effect on various aphids.

The invention belongs to the field of intelligent medical treatment, and particularly relates to a construction method of a virus single-gene host adaptability prediction model, equipment and a medium. The method comprises the steps that a training set virus single gene data set and a host tag are obtained, single genes in the virus single gene data set are sequentially encoded to obtain an encoding feature matrix set, and encoding comprises the steps that a sliding window method is used for traversing a gene sequence, frequencies of 64 different codons in a window form a window codon frequency vector, and the window codon frequency vector is used for encoding the host tag; representing a central codon of a window by using the window codon frequency vector, and representing each codon of a gene sequence as a window codon frequency vector in sequence; and inputting the coding feature matrix set into a residual network model to obtain a prediction label, and obtaining a single-gene host adaptability prediction model based on comparison iteration training of the prediction label and a host label. According to the method, the model capable of predicting the gene adaptability is obtained by training after the codon is represented, and the gene codon adaptability can be analyzed.

The invention discloses a double-selenium lipid nanoparticle as well as a preparation method and application thereof. According to the nanoparticle, a skeleton is composed of cationic lipid, neutral phospholipid, cholesterol and functionalized PEG lipid, meanwhile, mRNA and a TLR agonist for coding therapeutic protein are carried, and the nanoparticle is synthesized through an ethanol injection method; the nanoparticle has both active targeting and strong transfection capabilities, the lipid nanoparticle is actively targeted to a tumor microenvironment and is taken by tumor cells by using biotin modified PEG lipid, and rapid'inclusion-lysosome 'escape is realized in the tumor cells by using trifluorogroup modified PEG lipid. Meanwhile, the nanoparticles are degraded in a glutathione / hydrogen peroxide dual-mode, so that the entrapped medicine is quickly and effectively released; the drug delivery system provided by the invention is simple in synthesis method, high in gene transfection efficiency and good in biological safety, and has potential value in anti-tumor research and clinical application.

The invention belongs to the technical field of gene editing, and discloses a construction method of a uterus specific conditional gene editing mouse based on a CRISPR-AsCas12a system. According to the invention, the efficient editing ability of AsCas12a is innovatively combined with the uterus tissue specific Pgr promoter. According to the invention, rapid and efficient single gene knockout, such as Ythdf1, Ythdf2 and Ythdf3, is realized in the uterus. The technology breaks through the limitation of long breeding period of the traditional Cre / loxP system, and tissue-specific knockout can be realized without homozygosis. The CRISPR / AsCas12a system provides an animal model for researching pregnancy-related diseases and hormone regulation mechanisms and developing a uterus targeted gene therapy. Reference is provided for exploring other gene editing model animals, and development of gene editing animal models in the veterinary field is broadened.

The invention discloses an efficient streptomyces mobaraensis CRISPR / Cas9 editing method, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 system in streptomyces mobaraensis is successfully constructed and optimized, a related gene ssti of aerial hypha growth delay is selected as a reporter gene, a CRISPR / Cas9 editing system is optimized, an optimal promoter P19570 of Cas9 and the most appropriate sgRNA of a targeted ssti gene are obtained, and on the basis, a repair template with the length of 2kb is selected, so that the highest single gene editing efficiency reaches 90.8%. Furthermore, an array containing two gRNA expression cassettes is constructed, simultaneous knockout of ssti and sapB genes in smY2019 is realized, and the knockout efficiency is 6.25%.

The invention provides an MHC presentation peptide fragment prediction method and system based on multi-modal deep learning. The method comprises the following steps: constructing a multi-species MHC-peptide fragment combination data set; carrying out single genotype analysis on the multi-allele MHC-peptide fragment data in the multi-species MHC-peptide fragment combination data set, and determining a unique MHC-peptide fragment corresponding relationship; performing multi-sequence alignment and feature alignment on the cross-species MHC sequences, and extracting core site information representing MHC molecular polymorphism by adopting a frequency weighted amino acid feature distance algorithm; and constructing a multi-modal deep learning model, integrating MHC sequence features and peptide fragment sequence features, and predicting the binding capacity of MHC molecules and peptide fragments. According to the method, the high-order interaction characteristics between the MHC and the antigen peptide fragment can be more comprehensively and effectively captured.

The application belongs to the field of intelligent medical treatment, and particularly relates to a method for constructing a virus single-gene host adaptability prediction model, equipment and a medium. The method comprises the following steps: obtaining a training set virus single-gene data set and a host label; encoding the single genes in the virus single-gene data set in sequence to obtain an encoding feature matrix set; the encoding is as follows: using a sliding window method to traverse a gene sequence, the frequency of 64 different codons in a window is used to form a window codon frequency vector, the window codon frequency vector is used to represent a central codon of the window, and each codon of the gene sequence is represented as a window codon frequency vector in sequence; inputting the encoding feature matrix set into a residual network model to obtain a prediction label, and iteratively training based on a comparison between the prediction label and the host label to obtain a single-gene host adaptability prediction model. The model capable of predicting gene adaptability is trained by using the representation of codons, and the analysis of gene codon adaptability can be realized.

In certain embodiments methods of treating X-Linked agammaglobulinemia (XLA) in a mammal are provided where the methods comprise: i) providing differentiated T cells and / or stem / progenitor cells from the mammal; ii) performing a targeted insertion of a corrective BTK cDNA at the BTK gene locus in said cells to provide a corrected BTK gene in said cells; and iii) introducing said cells into said mammal where said corrected BTK gene is expressed in a physiologically regulated manner.

The present invention discloses a genome-wide association analysis algorithm at the gene level based on an EMS population, which relates to the technical field of bioinformatics. The present invention conducts association analysis with genes as the basic unit. The MAF index of SNPs in the EMS population is much lower than that of the normal population, but the MAF value at the gene level is higher than that at the SNP level. Weights are assigned according to the mutation effects of each mutation site, and the total weighted value of all mutations in each gene of a single sample is statistically calculated as the basis for association analysis, greatly reducing the false positive rate of the results and increasing the statistical power of the analysis. At the same time, multiple statistical methods are used for comprehensive evaluation to find the most reliable candidate genes. Compared with GWAS that can only locate a fuzzy interval that may contain multiple genes, the present invention can directly and accurately locate to a single gene, improving the experimental efficiency of functional verification of candidate genes and effectively solving the problems that the prior art cannot afford large-scale analysis of the EMS population and has low analysis power.

The present invention discloses a primer probe set and a kit for fluorescent quantitative PCR for detecting influenza D virus and its lineages. The primer probe set consists of a group of primer probes IDV PB-F, IDV PB-R, IDV PB-Prob for detecting influenza D virus, and a group of primer probes IDV HEF-F, IDV HEF-R, IDV HEF-Prob for identifying lineages. The primer probe set can realize differential diagnosis of five lineage strains of IDV in one reaction, which can not only confirm whether the influenza D virus is infected, but also reveal which lineage the detected strain belongs to, and is more efficient and accurate than single gene detection. The kit developed based on this has a good application prospect, and truly realizes a fully enclosed and pollution-free integrated reaction from "sample in" to "result out".

The application provides a personalized cancer driver gene identification method based on network propagation, and the implementation steps are as follows: obtaining related data of cancer driver gene identification; performing data fusion on abnormal genomics data; constructing a cancer gene regulation network of a group; constructing a personalized cancer gene regulation network; calculating influence score of a node pair; and obtaining a personalized cancer driver gene identification result through a network propagation process. In the process of constructing the personalized cancer gene regulation network, statistical analysis is performed on the interaction between each pair of genes of each patient, so that the deviation of the network model caused by abnormal expression of a single gene in the prior art is avoided; and in the process of network propagation, various genomics data and topological information in the network are integrated, the information abundance in the network model is improved, and the accuracy of identifying personalized driver genes is effectively improved.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

The invention relates to a full-length sequence amplification primer, an amplification method and a three-generation sequencing method of an HLA-I antigen gene, and belongs to the field of gene detection. The HLA-I antigen gene is subjected to PCR (Polymerase Chain Reaction) amplification by using 8 pairs of characteristic amplification primers. And each gene can be subjected to high-yield specific amplification on a full-length sequence containing complete intron and exon regions only through one round of amplification and enrichment, and 7.8 Kb long-fragment genes can be captured once at most. The experimental deviation caused by simultaneous amplification of a single gene by using a plurality of pairs of segmented primers and the indirect error caused by gene splicing are effectively avoided. The method comprises the following steps: carrying out quality inspection and purification on amplicon PCR (Polymerase Chain Reaction) products, mixing samples, constructing an HLA-I type antigen gene library, carrying out accurate and complete sequencing on the full-length sequence of the HLA-I type antigen gene by adopting a three-generation PacBio Sequel II platform, and ensuring that the Hifi reads base accuracy can reach 99% or above. The reading of HLA-I type antigen gene full-length sequence variation information and the haplotype analysis at a high resolution level can be realized.

The present application relates to the field of plant biotechnology, and in particular to a method for enhancing the nicotine synthesis level of tobacco, which comprises simultaneously overexpressing arginine decarboxylase gene NtADC1 (SEQ ID NO: 13) and transcription factor gene NtMYC2 (SEQ ID NO: 14), transforming tobacco, and obtaining a co-expression plant through hybridization. Experiments show that the nicotine content of the co-expression plant after topping is 310% of the wild type, which is significantly higher than that of the single gene overexpression plant (NtMYC2 overexpression increases by 35%, and NtADC1 overexpression increases by 5%). The method solves the technical bottleneck of the prior art that the nicotine improvement range is less than 40%, and provides a new scheme for high nicotine tobacco breeding.

This invention belongs to the field of genetic engineering and cell modification technology, and discloses a method for constructing and applying anti-aging human mesenchymal stem cells (MSCs). The DHX9-overexpressing virus-transfected MSCs constructed in this invention possess stable DHX9 overexpression effects and anti-aging functions. These MSCs do not target a single gene locus, but rather alleviate a series of aging phenotypes and genomic instability issues during the aging process by eliminating R-loops. DHX9-overexpressing MSCs exhibit significant anti-aging activity, as well as strong environmental adaptability and excellent safety characteristics. They can effectively resist harsh in vivo inflammation and the aging microenvironment, while avoiding the tumorigenic risk after cell transplantation. These MSCs with stable anti-aging capabilities can subsequently be used to significantly improve the efficacy of MSC infusion therapy for diseases.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

The invention provides modRNA (Ribonucleic Acid) for treating obliterated bronchitis, and belongs to the technical field of bioengineering. The modRNA for treating obliterated bronchitis provided by the invention is composed of an IL-10 modRNA (Interleukin-10) and an IFN (Interferon)-alpha modRNA (Interferon-alpha) modRNA. The BO treatment effect is achieved by inhibiting bronchial epithelial cell epithelial-mesenchymal transition (EMT), the expression of alpha-SMA and Vimentin in lung tissue can be remarkably reduced, the expression of E-cadherin and Cytokeratin 5 can be up-regulated, microbronchial epithelial injury is repaired, collagen deposition is reduced, and the lung function is improved. The synergistic effect of the two is better than that of single gene therapy, the safety is high, and the controllability is strong.

The application belongs to the technical field of molecular biology and medicine, and discloses a methylation marker for detecting the benignity and malignancy of thyroid nodules and an application. 780 new methylation sites are identified, 273 tumor occurrence and development related genes are associated, and the Fc gamma R-mediated phagocytosis signal pathway is involved. The methylation marker for detecting the benignity and malignancy of thyroid nodules comprises multiple genes and multiple methylation sites. The methylation level of multiple genes and multiple sites is detected, the problem of low and unstable methylation signal of single gene or single site is overcome, and thus the sensitivity and specificity of detection are improved.

The application discloses a kind of screening methods and application of pivot gene related to sheep muscle fatty acid content, to solve the technical problems that local sheep breed sample quantity is limited, traditional single gene analysis method is difficult to analyze fatty acid metabolism regulation from network level.This application carries out transcriptome sequencing to multiple months of muscle tissue of Gangba sheep, constructs gene expression matrix, using weighted gene co-expression network analysis (WGCNA) Combined with module characteristic gene and fatty acid phenotype correlation screening strategy, the pivot gene significantly positively correlated with muscle fatty acid content is obtained.The screening method can construct a robust co-expression network under limited sample size, systematically identify the functional module and core gene related to the content of fatty acids such as linoleic acid, and reveal the dynamics of fatty acid metabolism at different ages, and the method can be extended to other plateau livestock;The screened pivot gene can be used as a molecular breeding marker for early selection of Gangba sheep, and the breeding cycle is shortened.

The invention discloses an identification method, device and system for a high gene module scoring area, and relates to the technical field of space transcriptomics. The identification method comprises the following steps: acquiring space transcriptome data, dividing cells into a plurality of unit grids according to space coordinate information of the cells, and determining corresponding grid space coordinates for each unit grid; calculating a module score value of each cell and determining a high-score cell set; calculating an integrated gene set scoring index; and carrying out region division on the unit grid and determining a high gene module scoring region. According to the method, a plurality of genes are analyzed as a functional whole, the limitation that traditional single-gene analysis cannot reveal collaborative regulation is overcome, spatial quantization of a gene set synergistic effect is realized, and the problem that a gene set enrichment region cannot be positioned in the prior art is solved.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

The invention discloses a method for improving cell surface display efficiency and a product, and belongs to the technical field of biology. The invention provides a method for improving cell surface display efficiency. The method comprises the following steps: S1, increasing the size of a cell; knocking out genes in the cells to increase the size of the cells; the gene is selected from a group consisting of a PASchr < 2-10508 >, a PASchr < 1-40585 >, a PASchr < 30819 >, a PASchr < 2-20036 >, a PASchr < 2-10366 >, a PASchr < 1-40244 >, a PASchr < 30688 >, a PASchr < 1-10383 >, a PASchr < 1-40081 > and a PASchr < 40223 >. According to the method for improving the cell surface display efficiency, ten single-gene knockout strains and ten double-gene knockout strains are obtained based on the method, and experiments prove that the cell sizes of the strains are increased to different extents, so that the display efficiency, the display quantity and the enzyme activity are improved in the aspect of cell surface display, and the cell surface display efficiency is improved. Meanwhile, the report protein UGT73F24m-Ag alpha of the strains catalyzes GA to obtain a GLMG product, and the concentration of the GLMG product is increased to different degrees.

The invention belongs to the field of crop heredity, and relates to application of a polyphenol oxidase gene. The invention discloses an InDel marker for rice brown glume traits, a regulatory gene and application of the InDel marker and the regulatory gene. Based on a brown glume mutant material in the background of Hunan early indica 42, genetic analysis shows that the mutation is dominant mutation, single-gene control and gene positioning find that the mutant gene is a polyphenol oxidase encoding gene (MSULocus is LOCOs04g53300), and the exon region of the mutant gene is deleted by 6bp (GCGACT), so that the material shows brown glume; the gene is knocked out from a brown glume mutant, and the glume color becomes normal; the method has the advantages that the GCGACTs are used as the raw materials, the GCGACTs are accurately edited in the wild type materials, the corresponding 6bp deletion (GCGACT) materials are obtained, the glumes of the GCGACTs are brown, and new gene resources and methods are provided for quickly and directionally cultivating novel rice materials and rice materials suitable for mechanized seed production of hybrid rice.

The invention discloses a GPC1 gene and application thereof, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the GPC1 gene is shown as SEQ ID NO.2. The increase of the single gene expression quantity of the CPC1 gene leads to the significant increase of the whole grain protein content of the rice, but the knockout of the gene leads to the significant reduction of the whole grain protein content of the rice, and the whole grain protein content of the rice in a near-isogenic line of the allele has significant difference. The gene is used for improving the characters of rice varieties, has huge application potential and prospect, and provides a new gene resource for providing rice nutritional quality and cooking quality breeding.