32 results about "Obligate" patented technology

The present invention provides the biosynthesis of Schwertmannite as high efficiency adsorbent and its usage in adsorbing to eliminate Cr(VI) and As from waste water and underground water. Schwertmannite is prepared with ferrous sulfate as reactant, deionized water as reaction medium and Thiobacillus ferrooxidans LX5 as catalytic oxidant, and through stirring for 1-3 days, filtering to collect precipitate, washing with sulfuric acid acidified deionized water, washing with deionized water and stoving. Schwertmannite is added into water to be treated in the amount of 0.5 wt% or 0.02 wt%, dilute nitric acid is added into Cr(VI) containing system to regulate the pH value to 5.0-7.0 or sodium hydroxide is added into As containing system to regulate the pH value to 6.0-10.0, and the system is stirred for 3 hr and filtered to eliminate Cr(VI) and As. Schwertmannite has maximum adsorption amount on Cr(VI) and As of 55 mg / g and 65 mg / g separately.

One kind method and equipment for life sewage's denitrogenation and dephosphorization belongs to the water treatment domain. The method is that the sewage through obligate baffle reactor obligate sludge layer, spreads the phosphorus on the iron filings padding layer, remove the organic pollutant microorganism in obligate way, the not transformed organic pollutant is degraded in aerobic sunk-style biofilter biomembrane, the ammonia nitrogen transforms to nitrate nitrogen, then is removed again in obligate region by biomembrane counter- nitrobacter, again passes through the precipitation then discharges. The installment is made up of obligate baffle reactor mutually linking and in turn, the aerobic sunk-style biofilter and settling basin. The obligate baffle reactor compose of 3 - 4 baffle in, the partition board, obligate sludge separate room , 1st traverse has the iron filings padding layer, has counter- tube, in the aerobic sunk-style biofilter has the padding layer.

A novel microorganism capable of producing organic acids, Mannheimia sp. 55E, and a process for producing organic acid through anaerobic and aerobic incubation using the novel microorganism are provided. The method of producing an organic acid using the microorganism involves incubating Mannheimia sp. 55E with Accession Number KCTC 0769BP in a medium under anaerobic or aerobic conditions and obtaining an organic acid from the medium. Mannheimia sp. 55E produces succinic acid, lactic acid, and formic acid under anaerobic conditions saturated with CO2, and succinic acid, lactic acid, acetic acid, and formic acid under aerobic or N2 anaerobic conditions. Mannheimia sp. 55E is a facultative anaerobe tolerant of oxygen. Thus, the use of Mannheimia sp. 55E in producing organic acids can eliminate a problem of process instability, which would occur by the presence of oxygen in a fermentation process of producing organic acids using an obligate anaerobic microorganism.

The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.

The invention relates to the technical field of agricultural plant and especially relates to an obligate citrus AM inoculant and a preparation method thereof. According to the obligate citrus AM inoculant disclosed by the invention, obligate citrus AM fungi obtained from separation is mixed with sterile soil according to a proportion of 1 to (800 to 1000), citrus rootstock seedling growing is performed in the citrus AM fungi and the sterile soil for 12 to 16 weeks, the obligate citrus AM fungi are fully propagated and achieve full mutualism with citrus rootstock root systems, and the citrus rootstock root systems and cultivation soil of the citrus rootstock root systems are smashed to be packaged to obtain the obligate citrus AM inoculant. According to the obligate citrus AM inoculant disclosed by the invention, a mixing proportion of the obligate citrus AM fungi and the sterile soil is strictly controlled; furthermore, citrus rootstock seedling growing time is controlled; finally, theobligate citrus AM fungi inoculant which has high AM fungi content, good inoculation activity and appropriate proportion of mycelium outside roots to spore to mycelium inside the roots to endogenousAM mycorrhiza fungi can be obtained.

The invention relates to an efficient chlorobenzene degradation obligate bacterium, a composite microbial agent and a preparation method and application of the composite microbial agent, and belongs to the technical field of waste gas bio-purification. The efficient chlorobenzene degradation obligate bacterium is entitled a biological collection number: CGMCC No. 10053. The preparation method of the composite microbial agent comprises: mixing the efficient chlorobenzene degradation obligate bacterium with mixed bacteria at a mass ratio of (0.5-1):1 to obtain a composite strain, and inoculating and fermenting the composite strain until the viable count of fermentation broth ranges from 1.0 * 1010 cuf / mL to 1.0 * 1011 cfu / mL; mixing the fermentation broth and a solid carrier material at a ratio by weight of 1:(1-10) to obtain a composite microbial agent; the mixed bacteria are domesticated from activated sludge in an aeration tank of a wastewater treatment station of a petrochemical plant. The preparation method is simple and easy to perform; the composite microbial agent prepared by using the method is directly applicable to the startup of a waste gas bio-treatment system, is effective for degrading chlorobenzene waste gas and is easy to store and convenient to carry.

An object of the present invention is to provide an antitumor agent and / or a marker fur tumor detection with low invasiveness, few side effects, and high target specificity. Through the use of a recombinant obligate anaerobic gram-positive bacterium as an active ingredient containing a nucleic acid encoding a secretory fusion protein comprising a signal peptide, a low-molecular-weight single-chain antibody, and a functional peptide, an antitumor agent that enables delivery of a functional peptide to a target cell and / or a marker for tumor detection that enables monitoring of therapeutic effects over time are provided.

In order to reduce complexity and improve robustness of a safety monitoring system based on a CAN bus, the invention provides a CAN bus safety monitoring method comprising the steps that a plurality of monitoring devices are arranged on the CAN bus, wherein the monitoring devices are not connected with the CAN bus; a plurality of data transceivers are arranged at connection positions of the monitoring devices and the CAN bus, wherein the data transceivers are all connected with the CAN bus; each monitoring device is connected with one of the closest data transceivers; one data transceiver is set as a main node, and the other data transceivers are set as slave nodes; the main node sends detection information to the slave nodes, wherein the detection information comprises first obligate fields and second obligate fields; the slave nodes send feedback information to the main node after the slave nodes receive main node signals; and the main node receives the feedback information sent by each slave node. According to the method of the invention, balancing of requirements of the sensor and the interface of the CAN bus is avoided, the structure of the CAN bus safety monitoring system is simplified, and cost is reduced.

A liquid crystal display panel includes a display region, a periphery circuit region, a joint obligate region, a plurality of first test thin-film transistors (TFTs), a plurality of second TFTs, a plurality of first lines, a plurality of second lines, a blank region, and at least one first adjustment TFT. The first and second test TFTs are disposed on the joint obligate region according to a regular distance. Each of the first and second test TFTs has a transistor width. The first adjustment TFT is disposed on the blank region. The width of the blank region is not smaller than the sum of the twice regular distance and the transistor width. Thereby, the present invention can prevent the band mura of the liquid crystal display panel effectively when the liquid crystal display panel is in testing.

The invention discloses a data processing method and device for online analytical processing partitions. The data processing method for the online analytical processing partitions includes the steps that an analytical database for online analytical processing is divided into a first-stage obligate partition and a first target partition; analytical data for online analytical processing are obtained; whether target partition bodies for storing the analytical data exist in the first target partition or not is detected; if the target partition bodies for storing the analytical data do not exist in the first target partition, the first-stage obligate partition is divided to obtain a second target partition and a second-stage obligate partition, and the first-stage obligate partition and the second-stage obligate partition are empty storage partitions; the analytical data are stored into the second target partition. By means of the data processing method and device, the problem that in the correlation technology, when the tail partition is divided, data losing of front-terminal data querying will occur is solved.

The invention relates to feed for birds. In particular, the invention relates to types of nectar for consumption by obligate nectarivore and facultative nectarivore birds.

The invention discloses a strain of obligate halophile Y-B, which is obtained from well drilling waste mud by enrichment, screening and culture, and aims at realizing high-efficient degradation of petroleum in high-salt oily waste liquor in the petroleum industry. The strain Y-B is milky white, is of a gram-positive bacterium, is shaped like a cell rod and can produce spores, the strain Y-B is identified as Bacillus subtilis subsp. spizizenii and has very high activity, a culture method is simple, and the strain Y-B has the advantages of easiness in obtainment, low cost and rapid growth, is suitable for weakly-alkaline and high-salt environments, has a better effect of removing the petroleum in the high-salt oily waste liquor and further has broad application prospects in the treatment ofthe high-salt oily waste liquor in the petroleum industry and oil overflow sudden accidents.

The invention relates to a method for producing fuel ethanol by fermenting corncob processing residue. The method comprises the steps of (1) preparing an obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the compound enzyme preparation comprises endo cellulose, exo cellulose and beta-glucosidase; (2) adding the corncob processing residue to the obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the content of the compound enzyme preparation is 0.05-0.5 g in per gram cellulose; pre-enzymolyzing for 0-36 h at a temperature of 45-60 DEG C; then adjusting the temperature to 24-44 DEG C; adding 0.002%-0.005% of saccharomyces cerevisiae; and performing simultaneous sacchrification and fermentation on the corncob processing residue to produce ethanol. Non-crop fermentation raw materials can be supplemented during the fermentation process. Compared with a conventional method, the method provided by the invention solves the problem of low final ethanol concentration of a fermentation unit during the process of producing ethanol by fermenting the corncob processing residue, and reduces energy consumption of an ethanol concentrating stage.

The invention belongs to the technical field of pest attractants, and provides an obligate attractant for controlling oriental fruit moths and a control method. The effective components are sugar acetate and alcohol liquid and a host plant volatile matter which are mixed, wherein in the sugar acetate and alcohol liquid, the ratio of soft sugar to glacial acetic acid to absolute ethyl alcohol to water is 3 g:1 ml:3 ml:80 ml; in the host plant volatile matter, the ratio of nonanal to cis-3-hexenolacetate to 6-methyl-5-heptene-2-ketone to (Z)-beta-ocimene is 14:100:1:86, and the solubility of solvent n-hexane used for the host plant volatile matter is 1 mu g / mu l; the sugar acetate and alcohol liquid and the host plant volatile matter are mixed according to the volume ratio of 1:1. Through action of long-distance attraction of a food attractant and near-distance attraction of the oviposition attractant, not only is the control effect ensured, but also chemical pesticide reduction is propelled; green prevention and control product and technology popularization is accelerated; the obligate attractant and the method are beneficial to protecting the ecological environment and ensuring thefood safety and ecological safety and are beneficial to sustainable development of agriculture.

The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.

The invention discloses an obligate halophile fast culture method. A basic culture medium contains MnSO4.7H2O, ZnSO4.7H2O, Na2MoO4, AlK(SO4)2, H2BO4, CaCl2.2H2O and CoCl2.6H2O; an enriching culture medium contains beef extracts, peptone, NaCl, KH2PO4, agaragar and distilled water, and the pH value is 7.2 to 7.4. The phosphate buffer solution contains NaCl, KCl, Na2HPO4 and KH2PO4, and the pH valueis 7.2 to 7.4. The sludge obtained from a secondary sedimentation tank in a sewage plant is subjected to aerobic culture in the basic culture medium; the cultured sludge is subjected to enrichment culture by the enriching culture medium in a flat plate coating mode, and is put into a biomedical culture box to be cultured for 72h; the enriching culture medium is taken; surface layer sludge is scraped and taken; the phosphate buffer solution is used for repeatedly flushing; then centrifugation is performed by a high-speed centrifugation machine; supernatant fluid is removed; bottom layer sludgeis taken; the sludge is high-concentration halophilic sludge. The high-concentration halophilic sludge and the ordinary sludge are taken and proportionally added into a culture medium to be cultured.The method is used for fast culturing obligate halophile, so that the practical problems of biological treatment of high-salt waste water is solved.

The invention discloses a cultivating method of a wide spectrum pest-resistant transgenic rape, which comprises the following steps: firstly, obtaining object gene order, secondly, constructing antisense RNA expression vector, thirdly, doing genetic transformation, fourthly, testing transgenic plant, fifthly, identifying insect resistance, and finally, culturing insect resistance variety. The invention stops the translation of plant endogenous myrosin gene, eliminates or lowers the synthesis and activity of myrosin, complete glucosinolates in the bodies of plant can not be degraded, volatile chemical signal substance which can be identified by obligate pest can not be formed, thereby eliminating identification signal of obligate pest to crops, avoiding pest damage, or oviposition and breeding are inhibited even if feeding, thereby pests are effectively controlled. The cultivating method has permanent effect, wide scope and low cost, which has no public hazard.

One kind method and equipment for life sewage's denitrogenation and dephosphorization belongs to the water treatment domain. The method is that the sewage through obligate baffle reactor obligate sludge layer, spreads the phosphorus on the iron filings padding layer, remove the organic pollutant microorganism in obligate way, the not transformed organic pollutant is degraded in aerobic sunk-style biofilter biomembrane, the ammonia nitrogen transforms to nitrate nitrogen, then is removed again in obligate region by biomembrane counter- nitrobacter, again passes through the precipitation then discharges. The installment is made up of obligate baffle reactor mutually linking and in turn, the aerobic sunk-style biofilter and settling basin. The obligate baffle reactor compose of 3 - 4 baffle in, the partition board, obligate sludge separate room , 1st traverse has the iron filings padding layer, has counter- tube, in the aerobic sunk-style biofilter has the padding layer.

The present invention provides a method of treatment for cancer in a subject comprising the administration of a prodrug, wherein the subject is colonised with an obligate anaerobic microorganism capable of expressing a polypeptide having nitroreductase activity, wherein the polypeptide exhibits preferential reduction of CB1954 to a 4-hydroxylamine (4HX) derivative instead of a 2-hydroxylamine derivative; and is capable of reducing CB1954 to the 4HX derivative with a Km of less than 30 micromolar. The present invention further provides an isolated polypeptide, nucleic acids encoding said polypeptide, vectors and host cells comprising said nucleic acids and vectors and uses of the above in treating solid tumours.

The invention discloses orange II degrading bacterium L-15 and an inocula produced from the same. The orange II degrading bacterium L-15 has the bacterial strain of stenotrophomonas maltophilia, belongs to xanthomonas campestris of xanthomonadaceae, and is preserved in China General Microbiological Culture Collection Center since 11st, June, 2014, the preservation address is No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC NO.9289. The main biological characteristics comprise that the bacterium colony is circle and is not transparent, and degrading bacterium L-15 is obligate aerobic non-fermentation type Gram negative polar flagella bacillus, has strong ammonia flavor on a blood agar plate and has no hemolysis phenomenon. Degrading bacterium L-15 is capable of reducing the residual quantity of the azo dye orange II by 80% or more. The inocula has the advantages of low production usage cost, usage convenience and good removing effect, and is applicable to pollution treatment of water and soil.

The invention discloses a method for prolonging in vitro preservation duration of blumeriagraminisf .sp .tritici, and belongs to the technical field of application of preservation methods of plant pathogen obligate parasites. The blumeriagraminisf .sp .tritici, which belongs to plant pathogen obligate parasites, can survive with the existing of wheat leaf hosts. A method, discovered by the inventor, can achieve in vitro preservation of the blumeriagraminisf .sp .tritici without the assistance of hosts, however, the method has the shortcomings that the method is short in preservation duration,the method needs to collect a great amount of conidia and the like; on the basis, a strain resurrection inoculation mode is adopted, and blumeriagraminisf .sp .tritici spores undergo in vitro preservation through suspension of a perfluoroalkane solution, so that in vitro preservation duration of the blumeriagraminisf .sp .tritici spores is improved by six times in comparison with that of an original method, namely the in vitro preservation duration is prolonged to six years from original one year, and the mass of the required spores is reduced by four times. With the application of the methodprovided by the invention, shortcomings of existing methods can be overcome; and smooth implementation of related researches on blumeriagraminisf .sp .tritici pathogenic bacteria can be fully guaranteed.

The invention discloses a processing method of resource reservation renegotiation, a device and a system thereof, which are applied to the communication field, wherein, the method is applied to a system comprising a traffic flow management entity, a traffic flow authorized service point entity and a traffic flow authorized anchor entity, and the traffic flow management entity carries out resource degradation process to part of traffic flow; the method comprises the following steps: when the traffic flow management entity detects that the current wireless empty-export resource meets the restoration requirement of the degraded traffic flow resource at a mobile terminal, a notification message is sent to the traffic flow authorized anchor entity by the traffic flow authorized service point entity so as to trigger the traffic flow authorized anchor entity to launch a traffic flow modification process; the invention solves the problem in the prior art that the traffic flow of users is not updated in time as the resource negotiation is not re-processed, although the empty-export resource is released, thus perfecting the traffic flow process mechanism.

The invention discloses a processing method of resource reservation renegotiation, a device and a system thereof, which are applied to the communication field, wherein, the method is applied to a system comprising a traffic flow management entity, a traffic flow authorized service point entity and a traffic flow authorized anchor entity, and the traffic flow management entity carries out resourcedegradation process to part of traffic flow; the method comprises the following steps: when the traffic flow management entity detects that the current wireless empty-export resource meets the restoration requirement of the degraded traffic flow resource at a mobile terminal, a notification message is sent to the traffic flow authorized anchor entity by the traffic flow authorized service point entity so as to trigger the traffic flow authorized anchor entity to launch a traffic flow modification process; the invention solves the problem in the prior art that the traffic flow of users is not updated in time as the resource negotiation is not re-processed, although the empty-export resource is released, thus perfecting the traffic flow process mechanism.

The invention relates to a liquid fermentation tank inoculation device of obligate anaerobes, and an inoculation method, and more specifically relates to a gas flowing pipeline. The liquid fermentation tank inoculation device of obligate anaerobes comprises 1, a double-way plug provided with two through holes; 2, a flow inlet pipe which is designed to pass through one of the two through holes, wherein the external wall of the flow inlet pipe is connected with the through hole through gas tightness connection, the flow inlet pipe is provided with a flow inlet pipe main valve; and 3, a flow outlet pipe designed to pass through the other through hole, wherein the external wall of the flow outlet pipe is connected with the through hole through gas tightness connection, and the flow outlet pipeis provided with a flow outlet pipe main valve; the flow inlet pipe is provided with a branch pipe in front of the flow inlet pipe main valve, the branch pipe is provided with a flow inlet pipe blow-down valve; the flow outlet pipe main valve is provided with a branch pipe in front of the flow outlet main valve, and the branch pipe is provided with a flow outlet pipe blow-down valve. The liquid fermentation tank inoculation device contains the gas flowing pipeline.

There is provided inter alia use of 2′,3′-dideoxy-3′-hydroxymethylcytidine or a prodrug or salt thereof in the manufacture of a medicament for the treatment of HIV infection wherein the reverse transcriptase of the HIV bears at least one mutation that allows an obligate chain terminating nucleoside- or nucleotide phosphate to be excised from the nascent DNA strand by ATP- or pyrophosphate-mediated excision.

A preparation method of a high power capacity lithium ion battery cathode material uses natural spherical graphite to serve as raw materials, uses concentrated sulfuric acid to serve as an inserting layer agent, uses potassium permanganate to serve as an oxidizing agent, conducts expansion treatment at high temperature to obtain micro-expanded graphite, then enables the micro-expanded graphite in different proportions to be mixed with nanometer ganister sand, conducts ultrasonic dispersion, suction filtration and dying to obtain the micro-expanded graphite with the nanometer ganister sand inserted in layers, enables the micro-expanded graphite to be mixed and coated with carbon source carbon source according to certain proportion, and finally conducts carburizing sintering under inert gas shielding to prepare completely coated silicon carbon composite cathode material with sufficient obligated obligate inside. Electrochemistry shows that the silicon carbon composite material prepared by the preparation method has high specific capacity and cycling stability and is the ideal high power capacity lithium ion battery cathode material.