Obligate halophile for processing high-salt oily waste liquor in petroleum industry

Abstract

The invention discloses a strain of obligate halophile Y-B, which is obtained from well drilling waste mud by enrichment, screening and culture, and aims at realizing high-efficient degradation of petroleum in high-salt oily waste liquor in the petroleum industry. The strain Y-B is milky white, is of a gram-positive bacterium, is shaped like a cell rod and can produce spores, the strain Y-B is identified as Bacillus subtilis subsp. spizizenii and has very high activity, a culture method is simple, and the strain Y-B has the advantages of easiness in obtainment, low cost and rapid growth, is suitable for weakly-alkaline and high-salt environments, has a better effect of removing the petroleum in the high-salt oily waste liquor and further has broad application prospects in the treatment ofthe high-salt oily waste liquor in the petroleum industry and oil overflow sudden accidents.

Description

technical field [0001] The invention relates to an original halophilic bacterial strain and its application, in particular to the application of the bacterial strain in the treatment of different types of high-salt oily waste liquids. Background technique [0002] At present, oil plays a pivotal role in the development of the national economy. With the rapid development of society and regional economy, the country's demand for oil energy continues to increase, making oil fields explored and developed on a large scale, and the pollution problems caused by oil development have become increasingly prominent and serious. [0003] In the process of petroleum exploration and development, different types of oily waste liquids are produced in drilling operations, downhole operations, oil production, and combined stations. Due to the different characteristics of crude oil and geological conditions, the properties of waste liquids are quite different, but they also have common charact...

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Problems solved by technology

If the high-salt oily waste liquid is not properly treated or discharged directly, it will cause serious pollution to the environment. Excessively high pH value, high concentration of soluble salt and petroleum substances will affect the structure of the soil, resulting in soil compaction, making the soil unable to Returning to farmland will endanger the normal growth of plants; harmful petroleum substances may enter the food chain, posing a threat to the health and life safety of organisms in the environment and human beings

[0004] The treatment of high-salt oil extraction waste liquid mainly includes physical, chemical and biological methods, but the physical and chemical methods have the disadvantages of incomplete treatment, high cost, easy to cause secondary pollution, etc., so it is difficult to promote in practice. , low investment, low operating costs, no pollution to the environment and other advantages, it has increasingly attracted the attention and attention of environmentalists from all over the world, and has become a hot spot and focus of research in the field of ecological environment at home and abroad.

In recent years, the use of microbial methods to treat petroleum pollutants in the environment has been carried out successively at home and abroad, mainly to screen highly efficient degradation microorganisms or dominant microorganisms from the polluted environment, and then put them into the sample or environment to be treated to make them remove petroleum pollutants. Pollutants, however, the degradation of petroleum pollutants by microorganisms is easily affected by pH, salinity, etc. High salinity will reduce the activity and metabolism of microorganisms, thereby inhibiting the degradation of petroleum pollutants by microorganisms. In order to accelerate the degradation rate of petroleum pollutants, Measures are often required to enhance the fitness of microorganisms in adverse environments

Oilfield oily waste liquid usually has the characteristics of high salt content and high salinity, which brings certain difficulties to the treatment of ordinary microorganisms

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