The invention discloses an immuno-electrochemical paper chip. The immuno-electrochemical paper chip comprises a bottom plate, a reference electrode layer and a working electrode layer, wherein the working electrode layer comprises a plurality of central shunting regions, a plurality of micro-channels, a detection region and a plurality of working electrodes; each micro-channel is used for leading a sample to be detected from the detection region to the corresponding central shunting region; each working electrode is positioned in the corresponding central shunting region; different antibodies are fixed onto the surfaces of the working electrodes and are used for performing multi-parameter joint detection on FSH, LH and E2 respectively. The immuno-electrochemical paper chip is in a multi-layer combined design, functions of sampling, separating, immuno-detection and the like are achieved on a single paper chip, the operation is simple, and the detection is rapid; meanwhile, an electrochemical immuno-detection method is directly adopted, secondary antibodies do not need to be added, needed reagents are few, and manual operation errors can be reduced.