Immuno-electrochemical paper chip electrode

Abstract

The invention discloses an immuno-electrochemical paper chip. The immuno-electrochemical paper chip comprises a bottom plate, a reference electrode layer and a working electrode layer, wherein the working electrode layer comprises a plurality of central shunting regions, a plurality of micro-channels, a detection region and a plurality of working electrodes; each micro-channel is used for leading a sample to be detected from the detection region to the corresponding central shunting region; each working electrode is positioned in the corresponding central shunting region; different antibodies are fixed onto the surfaces of the working electrodes and are used for performing multi-parameter joint detection on FSH, LH and E2 respectively. The immuno-electrochemical paper chip is in a multi-layer combined design, functions of sampling, separating, immuno-detection and the like are achieved on a single paper chip, the operation is simple, and the detection is rapid; meanwhile, an electrochemical immuno-detection method is directly adopted, secondary antibodies do not need to be added, needed reagents are few, and manual operation errors can be reduced.

Description

technical field [0001] The invention relates to the technical field of test paper detection and paper chip, in particular to an immunoelectrochemical paper chip electrode for multi-parameter rapid detection of sex hormones. Background technique [0002] Assessing the function of the ovary is the key to understanding the reproductive health of women. Clinically, the method of detecting ovarian reserve function is often used to achieve it. Ovarian reserve function monitoring is used in the diagnosis of ovarian diseases (such as premature ovarian failure, precocious puberty, menstrual disorders, polycystic ovary syndrome, and perimenopause, etc.), the evaluation of treatment effects (such as evaluation of ovarian response to ovulation induction therapy), pregnancy guidance and women's Physiological health and so on are of great significance. At present, clinical evaluation of ovarian reserve mainly refers to clinical indicators such as age, basic follicle stimulating hormone (...

AI Technical Summary

Problems solved by technology

[0006] However, most of the paper chips using the direct electrochemical detection method are drop-dropped samples, and the sample volume is large. It is necessary to solve the problem of reducing the amount of samples used, reducing the mutual interference of different parameters in the detection of different parameters, optimizing the microchannel, micro The key scientific issues of the microstructure such as the reaction zone; the content of sex hormones in the body is extremely low, and the direct electrochemical immunoassay paper chip is susceptible to interference from electrode preparation and modification conditions and environmental factors. It is necessary to solve the quantitative detection of ultra-trace sex hormones, electrode surface modification and Scientific issues such as the preparation of biosensitive membranes

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