242 results about "Sample volume" patented technology

A sample preparation instrument with an integrated device for estimating a concentration of white blood cells in a specimen is described. The sample preparation instrument receives a specimen for which a sample is to be prepared for analysis. The integrated device can implement an optical method, an electrical resistance method, or a flow cytometry method, for example, to estimate the white blood cell concentration in the specimen. For some types of analysis, a sample volume of the specimen is dependent on the white blood cell concentration. Therefore, the sample preparation instrument can automatically determine and adjust the sample volume of the specimen based on the estimated white blood cell concentration prior to adding additional reagents, such as labeling reagents and / or lytic reagents, to prepare the sample.

A method of estimating an amount of a sequestered carbon-based constituent in a volume of soil that includes taking a core soil sample of a sample volume of soil proximate at and below a root system associated with carbon soil accumulation from a biomass. The core sample is taken at a depth extending from an earthen surface, the depth having a depth range. The core sample is tested to provide a data set.

The invention provides a detection method of an end face integrated reflection type SPR optical fiber biosensor manufactured based on a 2PP technology. An experiment system mainly comprises a single-mode optical fiber, an optical fiber coupler, an SPR probe, a tungsten lamp light source, a sample pool, a spectrum analyzer and a computer. The method is characterized in that a volcanic three-dimensional microstructure is directly constructed on the end face of a single-mode fiber through a 2PP technology, a metal film with the thickness of about 50nm is deposited on the inner wall of the volcanic three-dimensional microstructure, and a stable and efficient SPR excitation structure is formed; the structural parameters are subjected to FDTD simulation optimization design, and have the characteristics of high quality factor and high sensitivity. The sensor can be used for AFP detection, the sensitivity reaches 0.684 nm / (ng / mL) within the linear range of 0-10 ng / mL, the normal and risk early warning concentration interval of human serum AFP is covered, and the sensor has good biological suitability and early hepatocellular carcinoma screening capacity; in addition, the probe structure is small in size, detection can be completed only by a few microliters of liquid to be detected, and the probe is suitable for a microfluidic platform with an extremely low sample size or a clinical sampling scene. Compared with the prior art, the device has the advantages of high structural integration, high sensitivity, low sample consumption, convenience in manufacturing, stability, reliability and the like.

The invention relates to a TL-AEAT-BIGRU post-compression yield prediction method based on data joint driving under knowledge constraint, which adopts a CWGAN-GP model based on conditional constraint to perform data enhancement on a small amount of multi-source data, adopts a Pearson + mRmR correlation analysis algorithm to determine main control factors influencing post-compression yield, and performs prediction on the post-compression yield by using a TL-AEAT-BIGRU model. And classifying reservoir categories through a main component analysis method based on the reservoir classification standard established by the former. Correlation experience knowledge between the post-compression yield and the input main control factors is combined with the TL-AEAT-BiGRU post-compression yield prediction model, and the screened main control factors are used as input for post-compression yield prediction. The result shows that compared with other yield prediction models, the model has the best performance in the post-pressure yield prediction of the research area. An ablation experiment shows that each module of the model contributes to improvement of the yield prediction effect, so that the yield prediction accuracy of the model is effectively improved, the problem that the yield prediction precision of the reservoir after pressure is not high under the condition that the sample size is small is effectively solved, and the method has guiding significance on yield analysis of an oil and gas well.

The invention provides an on-site water sample micro-plastic multi-dimensional information integrated detection device and method, and relates to the technical field of micro-plastic pollution monitoring, and the device comprises a microscopic image identification and analysis module, a sample conveying module, a sample suction filtration module, a gas path transmission module, an electromagnetic thermal cracking module, a filtration purification module and a mass spectrometry detection module, the sample conveying module, the sample suction filtration module and the gas path transmission module are respectively communicated with the electromagnetic thermal cracking module, the filtration and purification module comprises a solid filtration and purification module and a gas filtration and purification module, and the sample conveying module, the electromagnetic thermal cracking module, the solid filtration and purification module and the sample suction filtration module sequentially form a sample liquid phase communication path. The microscopic image recognition and analysis module obtains a sample image at the sample conveying module. The particle size distribution and number information borne by the image and the type and mass concentration information borne by the thermal cracking mass spectrum are synchronously obtained, and the method has the advantages that the needed sample amount is small, information is not crossed, and pollution in the asynchronous information obtaining process is avoided.

A system to determine therapeutic infusion volumes of a therapeutic infusate to administer to a target putamen of a subject to achieve a desired percent coverage of the therapeutic infusate within the target putamen. The system includes an input interface, a storage device, a processor, and output interface. The input interface receives data on a target putaminal volume of the target putamen, a number of target segmental regions of the target putamen, and the desired percent coverage. The storage device includes sample putaminal volumes, sample therapeutic infusion volumes, sample volumes of distribution, and a sample percent coverage. The processor determines the total therapeutic infusion volume of the therapeutic infusate to infuse into the target putamen. The output interface provides the total therapeutic infusion volume of the therapeutic infusate to achieve the desired percent coverage of the therapeutic infusate.

The disclosure invention relates to the field of bio / chemical sampling, sensing, assays and applications. Particularly, the disclosure relates to how to make the sampling / sensing / assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g., 0.5 μL or less), operated by a non-professional, reading by mobile-phone, or low cost, or a combination of them.

The invention relates to the technical field of automatic control, and discloses a PLC-based seawater multi-nuclide separation automatic control method and system, and the method comprises the steps: obtaining analysis task parameters, such as a sample volume, a target nuclide type sequence, and the like; converting the data into an internal execution formula containing a precise time sequence and an actuator action sequence through PLC internal compiling logic; the method comprises the following steps: performing online pretreatment on original seawater based on sensor feedback of pH value, flow velocity and the like to obtain a sample flow with optimized conditions; according to system state data, multi-nuclide sequential adsorption is realized, and a separation column loaded with target nuclide is obtained; and sequentially eluting by using different eluents, and collecting to obtain the purified single nuclide component. The whole process runs in a full-automatic mode, the risk that experimenters are exposed to the radioactive environment is greatly reduced, errors caused by manual operation are effectively eliminated, and high consistency and traceability of analysis data of different batches and different monitoring mechanisms are guaranteed.

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer group and a kit for simultaneously detecting multiple pathogenic microorganisms and application of the primer group and the kit in detection of mouse-borne pathogenic microorganisms. The nucleotide sequences of the primer group are as shown in SEQ ID NO: 1 to SEQ ID NO: 122. In each PCR detection system, when the template quantity reaches 20 copies or more concentration, 22 pathogenic microorganism mock plasmids can be completely detected, which indicates that the primer group can effectively detect pathogenic microorganisms in mice and parasites carried by the mice, and the detection sensitivity is equivalent to that of PCR. Meanwhile, the detection method has the advantages of rapidness, high efficiency, low cost, wide and accurate detection range and capability of meeting the requirements of high sensitivity and low sample size.

The invention discloses an immunochromatography test strip based on a nano-robot as well as a preparation method and application thereof. The immunochromatography test strip comprises a bottom plate, and a sample pad, filter paper, a nitrocellulose membrane and absorbent paper which are sequentially overlapped and adhered to the bottom plate, when in use, an FPSN (at) Ab buffer solution is dropwise added on the surface of the sample pad; the FPSN (at) Ab buffer solution is prepared from Fe3O4 (at) mPDAamp; the preparation method comprises the following steps: preparing an mSiO2 (at) Nanozyme nano composite material and an antibody to be marked; the Fe3O4 (at) is mPDAamp; the mSiO2 (at) Nanozyme nano composite material is nano particles with an asymmetric structure, one end of the asymmetric structure is a core-shell structure, the core is Fe3O4, the shell is mesoporous polydopamine particles, the other end is rod-like mesoporous SiO2, and noble metal nano particles are loaded on the surfaces of Fe3O4 and mesoporous SiO2; according to the invention, the nano-robot is used as the probe to construct the immunochromatography test strip, so that the detection sensitivity and the detection speed of the test strip are improved, the binding efficiency between the probe and the to-be-detected object is optimized, and the problem that in the prior art, accurate detection of disease markers is difficult to realize under the conditions of small sample size and low concentration is solved.

The invention discloses a method for automatically collecting nucleic acid of aquatic microorganisms. According to the invention, a water sample is injected into a coagulation reaction bin with a temperature control function, and microbial cell lysis is realized through intermittent low-speed stirring; injecting a surface modification magnetic bead suspension into the reaction bin, starting a multi-shaft stirrer to realize efficient combination of a nucleic acid-magnetic bead compound, and synchronously starting temperature control in the bin to maintain RNA stability; by integrating high-precision positioning, pressure sensing and temperature control cracking core technologies, whole-process standardized operation is realized. The device adopts a dual-mode positioning and pressure sensor to ensure millimeter-level precision of geohydrological data of a sampling point; the three-dimensional turbulent flow field is combined with the magnetic separation array, so that the nucleic acid capture rate is greatly improved, and the risk of nucleic acid degradation is reduced as much as possible. According to the modularized flow path system, through three-stage gradient washing and nanometer ultraviolet sterilization, the problems that in traditional water sample collection, the sample size is large, information is prone to being lost, and treatment lags are effectively solved, and the overall collection efficiency and collection accuracy are improved.

The invention relates to the technical field of analysis and detection, in particular to a method for determining residual epichlorohydrin in dihydroxypropyl theophylline. The residual epoxy chloropropane in the dihydroxypropyl theophylline is detected by adopting GC-MS (Gas Chromatography-Mass Spectrometer); chromatographic conditions are as follows: a chromatographic column is VF-624ms, 30m * 0.25 mm * 1.4 mu m; the carrier gas is helium, the column flow velocity is 0.95-1.05 ml / min, and the split ratio is 10: 1; the temperature of a sample inlet is 230-250 DEG C; the sample injection volume is 1 microliter; mass spectrum conditions are as follows: an ion source: EI; the temperature of the ion source is 220-240 DEG C; the temperature of a quadrupole rod is 140-160 DEG C; and the temperature of the auxiliary heater is 250-270 DEG C. Compared with the prior art, the analysis method provided by the invention has the advantages of high sensitivity, strong separation capability and rapidness, and can accurately quantify epichlorohydrin in the test sample.

The invention discloses a secretion sampling device for gynaecology and obstetrics, relates to the technical field of medical instruments, and aims to solve the technical problem that an expansion structure of a traditional device adopts duckbill pliers, and the inner wall of a genital organ is easily mechanically stimulated or scratched, and the secretion sampling device comprises a fixed plate, an expansion mechanism, a scraping component, a gathering component and an extraction component. Through the gathering and expanding design of the expanding plate, the expanding plate is in a sealed barrel shape and small in diameter during gathering, so that the expanding plate can be safely put into the genital organ and protect an internal sample, and the inner wall of the genital organ protrudes through the gap during expanding to expose a sampling area; by matching with the bending surface and the back hook structure of the scraping plate, inner wall samples can be efficiently scraped during rotation, the samples are synchronously sucked by the cotton slivers, and meanwhile, the samples can be pushed to be gathered, so that sufficient sample quantity is ensured; safe placing, accurate scraping, efficient collection and sample protection in the sampling process are integrally achieved, the device is suitable for different sampling scenes, sampling convenience and sample integrity are improved, and subsequent detection reliability is guaranteed.

A microtiter plate assembly is disclosed that includes a base having a plurality of wells and a lid having a plurality of projections corresponding to the plurality of wells. Each projection contains a radial notch and a canted distal tip, providing bubble free sealing, reduced oxygen back-diffusion, and increased sensitivity even at small sample volumes.

The invention relates to a device (1) for measuring clouds and fog, comprising a first optical particle sensor (2) for the time-resolved measurement of at least one parameter of particles (3) in a first sample volume (4) of air from an environment (5), with a first volume flow measuring device (6) for determining a flow rate of the air through the first sample volume (4), wherein a computing device (7) is provided, by means of which a presence of a cloud and / or fog in the air can be determined from the at least one parameter.

A gas chromatograph (100) for analyzing content of a gas sample includes a sample gas inlet configured to receive the gas sample and a carrier gas source (114) which provides a carrier gas. A separation column (120) is configured such that individual component gases in the sample gas separate as they move through the separation column (120). A first sample valve (112) is coupled to the sample gas inlet, the carrier gas source (114) and to a sample loop which is configured to contain a sample volume of the gas sample. The first sample valve (112) injects the sample volume into the separation column (120) and individual component gases in the sample gas separate as they move through the separation column (120). A detector (122) detects a concentration of an individual component gas as a function of a sensitivity of the detector (122). A controller (108) changes a response factor of the gas chromatograph by changing at least one of the sensitivity of the detector (122) and the sample volume.

This invention discloses a micro-wedge splitting test device and a method for testing fracture parameters of quasi-brittle materials. The device includes a micro-sample, a groove on the upper part of the micro-sample, and a force-transmitting component. The force-transmitting component comprises a wedge, a roller, and a force-transmitting block connected in sequence. Compared with existing standard wedge splitting test devices, the ratio of the fracture cross-section to the volume of the micro-sample increases by more than 1.8 times, and the ratio of the shoulder width to half-width of the micro-sample decreases by less than 10%. By controlling the ratio of fracture cross-section to sample volume and the ratio of sample shoulder width to sample half-width, the device can reduce the size of the wedge splitting test device while ensuring that the crack forms and propagates stably at the center. Furthermore, by limiting the load rate to approximately 0.1 mm / min, the device ensures stable energy release during the micro-splitting test, making the micro-wedge splitting test device suitable for testing the fracture performance of small-sized functional components.

The invention belongs to the technical field of material detection, and particularly relates to a method for testing the specific surface area of a lithium-rich manganese-based positive electrode material. The testing method comprises the following steps: S1) heating and degassing pretreatment: adding a to-be-tested sample of the lithium-rich manganese-based positive electrode material into a sample tube, and then heating under a vacuum condition for degassing pretreatment to obtain the mass of the pretreated to-be-tested sample and the sample tube after degassing pretreatment; the temperature of the degassing pretreatment is greater than or equal to 300 DEG C; and S2) measuring the specific surface area by adsorption and desorption at low temperature: fixing the sample tube subjected to degassing pretreatment at the interface of a specific surface area tester, testing, and obtaining the specific surface area of the lithium-rich manganese-based positive electrode material according to the quality of the pretreated sample to be tested. Compared with the prior art, the test method provided by the invention has the advantages that the required sample amount is small, and the accuracy and the stability of the test are improved by carrying out heating and degassing pretreatment before the test.

This application belongs to the field of fluorescence microscopy imaging, and more specifically, relates to a dual-gel system suitable for dilatational microscopy of large-volume biological tissues. This dual-gel system achieves uniform expansion and morphological stability of tissue samples through a dilatational gel, and then imparts high mechanical strength using a stabilizing gel. This allows millimeter-scale large-volume biological tissue samples to possess both high expansion ratio and excellent mechanical stability, achieving 3-5 times linear expansion with a Young's modulus of not less than 300 kPa. It can stably adapt to vibrational cutting operations and support cutting of 100-500 μm thick layers, with intact cut surfaces and good interlayer consistency, greatly improving the efficiency and quality of three-dimensional data acquisition. Furthermore, the hydrogel sections obtained from the cutting have intact morphology and can be used as independent slides for multidimensional analysis. This application overcomes the limitations of traditional dilatational microscopy techniques in terms of sample volume and mechanical stability, providing an efficient and reliable solution for the three-dimensional reconstruction of millimeter-scale biological tissues.

A volumetric imaging system implements obtaining raw image data of a sample volume of a sample material, the raw image data comprising light field data of the sample volume acquired using a microlens array disposed in front of a camera; and analyzing the raw image data using an image analysis pipeline configured to localize the objects of interest in the raw image data to obtain classified image data in which the objects of interest have been identified, the image analysis pipeline being configured to process the raw image data to improve signal extraction at depth in the scattering material to maximize localization accuracy.

The invention relates to the technical field of molecular diagnosis, in particular to a high-sensitivity peripheral blood free DNA magnetic bead capture system, a methylation transformation method kit and application. The kit is composed of a lysis solution, a protease K diluent, a composite conversion reagent, a washing solution, a scavenger, an eluent and combined magnetic beads. According to the high-sensitivity peripheral blood free DNA magnetic bead capture system and the methylation transformation method kit, the detection limit of early-stage lung cancer ctDNA reaches 0.005% AF, the I-stage cancer ctDNA detection rate is increased to 93% from the industry average 40%, the sample size is reduced to 4 mL from the conventional 10 mL, the batch-to-batch difference of the kit is small, and the kit has very high application value clinically.

A system for verifying a sample volume includes a sample reservoir and a volumetric verification device. The sample reservoir defines an inner volume and is configured to receive a volume of bodily fluid. The inner volume of the sample reservoir contains an additive. The volumetric verification device includes a first indicator and a second indicator. The volumetric verification device is configured to selectively engage the sample reservoir to (1) place the first indicator in a first position along a length of the sample reservoir such that the first indicator is substantially aligned with a surface and / or meniscus of the additive and (2) place the second indicator in a second position along the length of the sample reservoir such that the second indicator is substantially aligned with a predetermined fill volume when bodily fluid is transferred to the inner volume.

The invention relates to a multifunctional liquid level detection method, which comprises the following steps of: 1, sending an instruction and initializing an MCU (Microprogrammed Control Unit) through an upper computer, and determining a detection task and an initial parameter; step 2, on the basis of the initialization parameters in the step 1, a sampling needle is put into a test tube to scan a capacitance detection module, and the capacitance detection module captures a capacitance change signal after the sampling needle is in contact with the liquid level; the sampling needle is of a double-needle-tube structure comprising a sampling needle tube and a mixing needle tube; 3, on the basis of the capacitance change signal in the step 2, liquid level position judgment and sample size state judgment are completed through MCU algorithm processing; and step 4, based on a sample size judgment result in the step 3, respectively completing mixing or direct sampling operation through function division and hardware cooperation of the sampling needle tube and the mixing needle tube. The method has the advantages that the accuracy is greatly improved, the anti-interference capacity is high, and the operation process is simplified.

The invention discloses a clinical specimen self-adaptive liquid taking device, and relates to the technical field of medical auxiliary equipment, and the clinical specimen self-adaptive liquid taking device comprises a mobile operation table, a volume measurement module, a processing module, a liquid taking metering module and a man-machine interaction module. According to the device, through deep fusion of the volume measurement module and the temperature compensation unit, the precision limitation of traditional manual estimation of the sample volume is broken through, the liquid and tissue density can be dynamically corrected according to the environment temperature, and accurate measurement of the sample volume is achieved; the processing module automatically calculates the amount of the stationary liquid meeting the industry specification based on the accurate volume data, and through real-time feedback and closed-loop control of an electromagnetic flowmeter of the liquid taking and metering module, it is ensured that the amount of the stationary liquid meets the requirement for complete immersion of the specimen, excessive waste is avoided, and the cost is reduced. Autolysis and deterioration of specimen tissues caused by insufficient stationary liquid are effectively prevented, a high-quality specimen basis is provided for pathological diagnosis, and the accuracy of diagnosis results is guaranteed.

Described herein are systems and methods that combine at least three different detection modalities to characterize the stability of drug formulations, and sample plates for use with the systems and methods. The systems may include a clamp configured to apply a force to the sample plates that trap and pressurize air above an inlet and / or outlet of sample holders included in the sample plates. Measurements of various parameters of the samples may be obtained in tandem or simultaneously with a single instrument, which reduces sample volume requirements.

This invention discloses a mass spectrometry ionization method based on the Tesla coil principle. It utilizes a Tesla coil to generate a micro-plasma beam, which serves as the ion source. Liquid samples are desorbed and introduced into the micro-plasma beam region for ionization, or gas samples are directly introduced into the micro-plasma beam region for ionization. This invention, by creatively using a Tesla coil-generated micro-plasma beam as the ion source, not only achieves strong ionization capability and good ionization effect on volatile gas samples and low-polarity or non-polar liquid samples, but also requires sample volumes as low as picoliters to microliters, exhibits good solvent compatibility and strong salt resistance, and further possesses advantages such as simple operation, low cost, ease of implementation, and broad applicability.

The present invention provides a kit for detecting or assisting in the detection of purine substances, comprising reagent 1, reagent 2, and reagent 3. Reagent 1 is a color developer, reagent 2 is a reaction enzyme, and reagent 3 is a weak acid salt; the reaction enzymes include peroxidase and xanthine oxidase. The kit also includes vitamin C and ascorbic acid enzyme; the vitamin C and ascorbic acid enzyme are packaged separately. The kit of the present invention is rapid and intuitive when detecting a sample to be tested; the kit of the present invention is used to detect purine substances without requiring any instruments or professionally trained personnel to obtain qualitative or semi-quantitative results; when the kit of the present invention is used for detection, even when the sample volume is large, the operation is not restricted, and the kit is easy to operate, improving the user experience.

The invention discloses an oil product moisture content detection automatic sampling control system and a moisture detection method.The oil product moisture content detection automatic sampling control system comprises an oil sample volume positioning system, an oil inlet of the oil sample volume positioning system is communicated with a to-be-detected oil product bottle through an oil inlet pump, and an outlet of the oil sample volume positioning system is communicated with an oil inlet of an oil sample moisture testing device; a gas inlet of the oil sample moisture testing device is communicated with an outlet of the gas inlet system, and the automatic sampling control system is connected with the oil inlet pump, the oil sample volume positioning system, the oil sample moisture testing device and the gas inlet system for realizing automatic sampling. The system provided by the invention can realize automation of flushing, sample introduction, sample layout and replacement of the oil product to be detected in the detection process.

This application provides a liquid analyzer and its automatic pipette tip selection detection method and storage medium. The method includes: acquiring consumable information and detection task information; the consumable information includes pipette tip model, available quantity of pipette tips, mixing cup specifications, and available quantity of mixing cups; the detection task information includes the detection items to be performed and the sample type; determining the sample volume and sample processing liquid volume that meet the pipetting conditions based on the detection task information and the detection method corresponding to the detection items; obtaining the pipetting coefficient of variation based on the sample type, sample volume, and sample processing liquid volume; selecting a target pipette tip model that meets preset detection conditions based on the pipetting coefficient of variation and the consumable information; and selecting the corresponding target pipette tip to perform the detection task and obtain the detection result. This method can reduce detection errors caused by improper pipette tip selection and effectively improve the accuracy and efficiency of detection.

This utility model belongs to the field of medical device technology and relates to an unfoldable nasopharyngeal swab. The unfoldable nasopharyngeal swab of this utility model includes: an outer protective sleeve with an arc-shaped curved front end and a handle at the rear end; a three-headed sampling swab composed of three parallel sampling swabs, each including a flexible swab rod and a sampling head; the central sampling swab passes through an inverted cone-shaped body to expose its front end swab rod and sampling head, while the two lateral sampling swabs extend from the sides of the inverted cone-shaped body, thus separating the sampling heads of the three sampling swabs; and a push handle connected to the rear end of the three-headed sampling swab. The unfoldable nasopharyngeal swab of this utility model solves the problems of small sampling range, easy missed sampling, and high operational risk in existing technologies through its three-headed coordinated unfolding design. It can accurately cover high-risk areas of nasopharyngeal carcinoma, significantly improve sampling efficiency and sample volume, and enhance the sampling effect and safety of nasopharyngeal swabs.