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56results about How to "Fast and accurate method" patented technology

Molecular and bioinformatics methods for direct sequencing

The present invention relates to methods for preparing an isolated biological sample containing at least one of DNA and RNA, such that the DNA and/or RNA is preserved in the sample at ambient temperatures for at least thirty days, the method comprising: contacting the isolated biological sample with a composition comprising a chaotropic agent, and subjecting the contacted sample to microbial cell lysis; and optionally, contacting the lysed biological sample with a slurry of size-selected silicon dioxide to form at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes in the sample; isolating at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes from the sample; and, separating at least one of DNA and RNA from the silicon dioxide and collecting at least one of the DNA and RNA.
The present invention further relates to methods for preparing an isolated biological sample, the method comprising, separating the components in an isolated biological sample according to their size, wherein the components are at least one of DNA and RNA; purifying and isolating SSU rRNA from the biological sample using a composition comprising a ribonuclease inhibitor and a deoxyribonuclease to remove DNA from the sample, reverse transcribing the SSU rRNA into ds cDNA using random primers for SSU rRNA.
The present invention also relates to computer implemented methods comprising, receiving an isolated sample prepared according to the methods of the invention, sequencing the sample, and providing the sequence with a sequence identifier (ID), the sequence comprising a plurality of groups of k-mers, each group of k-mers defining a node in a multi-level hierarchy which defines the relationship between the groups of k-mers; providing each group of k-mers with a respective group identifier (ID), determining the frequency of the k-mers in each group; generating a group signature array for each group of k-mers, each group signature array comprising the k-mers in each group that have the most increased frequency compared with the sibling k-mers; generating a signature map comprising each group signature array and at least one of the identifiers, the identifier of at least one parent group and the identifier of at least one child group; and outputting the signature map to be used to classify the sequence.
Owner:16S TECH INC

Method for calculating a local extremum, preferably a local minimum, of a multidimensional function E(x1, x2, ..., xn)

The invention relates to a Method for calculating a local extremum, preferably a local minimum, of a multidimensional function E(x1, x2, . . . , xn) which is interpretable as a function of potential energy with spatial coordinates (x1, x2, . . . , xn), using an iteration process based on a molecular dynamics based quenching method comprising the following steps:
a) Calculating a trajectory X(ti) at discrete times tiiδti starting from an assigned initial coordinate X(0) on basis of a gradient fk=δE/δxk with k=1, . . . ,n and a time derivatives of the coordinates vk=dxk/dt using fxk=mxk dvxk/dt in which mxk represent masses at the spatial coordinates and vxk=dxk/dt represents velocity
b) Performing a molecular dynamics based quenching method for analysing said function E(x1, x2, . . . , xn) of existence of a local extremum, in case of reaching a local extremum abort processing and/or select a new initial coordinate and proceed further from step a)
c) Calculating at each iteration time step δti
    • F=(fxk) and (k=1, . . . ,n) representing a global force vector field acting in the spatial coordinates,
    • V=(vxk) representing a velocity vector field, P=F·V representing power Setting V=(1−α)×V+α×(F/|F|)·|V|
    • α is a dimensionless variable and amounts a given initial value αstart at first iteration time step
    • In case of P<0: V is set to zero, α becomes αstart, δti will be reduced and return to step b)
    • In case of P≧0: Analysing whether the number of conducted iteration steps since the last detected case of P<0 exceeds a given minimum number Nmin, in case of “no” returning to step b) and in case of “yes” increasing δti, decreasing α and return to step b).
Owner:FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG EV

Multi-level matter-element entropy weight-based congestion hotspot airspace sector recognition method

The present invention discloses a multi-level matter-element entropy weight-based congestion hotspot airspace sector recognition method and belongs to the air traffic management field. The method includes the following steps that: a three-level airspace sector congestion degree evaluation index system including a target level, a dimension level and an index level is established, the calculation or sampling method of indexes is established, evaluation indexes are calculated on the basis of evaluation sector sample selection, and index level threshold judgment is performed according to index numerical value distribution; a multi-level matter-element model is established, association degrees and index weights are calculated, a sector congestion dimension level is judged; and the calculation of a sector congestion dimension weight is determined on the basis of an entropy weight method, and a congestion hotspot airspace sector is recognized. According to the method of the invention, a sector congestion degree is comprehensively judged from a plurality of dimensions, technical support is provided for the research and development of control auxiliary decision-making system tools, and references are also provided for airspace planning, sector adjustment and the like.
Owner:THE 28TH RES INST OF CHINA ELECTRONICS TECH GROUP CORP
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