56results about How to "Fast and accurate method" patented technology

A method for time aligning audio signal, wherein one signal has been derived from the other or both have been derived from another signal, comprises deriving reduced-information characterizations of the audio signals, auditory scene analysis. The time offset of one characterization with respect to the other characterization is calculated and the temporal relationship of the audio signals with respect to each other is modified in response to the time offset such that the audio signals are coicident with each other. These principles may also be applied to a method for time aligning a video signal and an audio signal that will be subjected to differential time offsets.

A method for determining if one audio signal is derived from another audio signal or if two audio signals are derived from the same audio signal compares reduced-information characterizations of said audio signals, wherein said characterizations are based on auditory scene analysis. The comparison removes from the characterisations or minimizes in the characterisations the effect of temporal shift or delay on the audio signals (5-1), calculates a measure of similarity (5-2), and compares the measure of similarity against a threshold. In one alternative, the effect of temporal shift or delay is removed or minimized by cross-correlating the two characterizations. In another alternative, the effect of temporal shift or delay is removed or minimized by transforming the characterizations into a domain that is independent of temporal delay effects, such as the frequency domain. In both cases, a measure of similarity is calculated by calculating a coefficient of correlation.

Methods, systems and kits for the simultaneous or sequential analysis of a multitude of steroid hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of steroid hormones are disclosed including ionization of the steroid hormones by photoionization.

A method for tomographic imaging of diffuse medium includes directing waves into a diffusive medium, solving a surface-bounded inversion problem by forward field calculations through decomposition of contributions from the multiple reflections from an arbitrary surface within the diffusive medium or outside the diffusive medium into a sum of different orders of reflection up to an arbitrary order, and using contact or non-contact measurements of waves outside said diffusive medium to generate a tomographic image.

A peripheral intravascular catheter assembly comprising a tubular introducer needle and an adapter connected to the proximal end of the light guide. An introducer needle comprises a connection hub and a needle member. The needle member has a lumen, a distal end and a proximal end. The assembly may be further provided with a cannula tubing surrounding at least a portion of the needle member, with at least one light source and with an electronic module, configured to power the light source. The light guide has a distal end and a proximal end and extends along the lumen of the needle member. The adapter may be connected to the electronic module to the connection hub of the introducer needle so that light emitted by at least one light source is capable of entering the light guide, but blood flowing through the lumen of the needle member is prevented from contacting said light source.

A method and system for volumetric quantification of local bone changes includes the steps of loading at least a first and second (cone-beam) computed tomography three dimensional image, registering the first three dimensional image and second three dimensional image to one coordinate system, selecting a region of interest in one of the first three dimensional image or the second three dimensional image. For the first three dimensional image and the second three dimensional image, segmenting the local bone within the region of interest by segmenting voxels related to air and / or soft tissues within the region of interest and attributing these voxels to an outside region, while the volume formed by the remaining volume represent the local bone or the local bone with soft tissue, and calculating of the volume of local bone in the first three dimensional image and the volume of local bone in the second three dimensional image and subtracting the first volume from the second volume and defining the difference as the local bone change or the change in local bone with soft tissue.

The invention discloses a method for recovering local music information based on audio fingerprinting technology. The method comprises the following steps of: firstly, creating a music database on a server side, wherein the music database takes audio fingerprints as the indexes of corresponding music information; inputting, by a user at a client, a local path of music files which are needed to be supplemented with missing information; extracting, by the client, the audio fingerprints of the music files in the path, and transmitting the audio fingerprints obtained to the server side; performing comparison lookup in the music database by the server side, and transmitting the information of music corresponding to the audio fingerprints to the client; and after the client obtains the missing information, providing the missing information for the user to look over, or directly modifying the music files, or rearranging the music files. With the method of the invention, the user can conveniently re-obtain various kinds of missing information of the local music files; and the method is not only simple and quick, but also accurate.

The present invention relates to methods for preparing an isolated biological sample containing at least one of DNA and RNA, such that the DNA and / or RNA is preserved in the sample at ambient temperatures for at least thirty days, the method comprising: contacting the isolated biological sample with a composition comprising a chaotropic agent, and subjecting the contacted sample to microbial cell lysis; and optionally, contacting the lysed biological sample with a slurry of size-selected silicon dioxide to form at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes in the sample; isolating at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes from the sample; and, separating at least one of DNA and RNA from the silicon dioxide and collecting at least one of the DNA and RNA.The present invention further relates to methods for preparing an isolated biological sample, the method comprising, separating the components in an isolated biological sample according to their size, wherein the components are at least one of DNA and RNA; purifying and isolating SSU rRNA from the biological sample using a composition comprising a ribonuclease inhibitor and a deoxyribonuclease to remove DNA from the sample, reverse transcribing the SSU rRNA into ds cDNA using random primers for SSU rRNA.The present invention also relates to computer implemented methods comprising, receiving an isolated sample prepared according to the methods of the invention, sequencing the sample, and providing the sequence with a sequence identifier (ID), the sequence comprising a plurality of groups of k-mers, each group of k-mers defining a node in a multi-level hierarchy which defines the relationship between the groups of k-mers; providing each group of k-mers with a respective group identifier (ID), determining the frequency of the k-mers in each group; generating a group signature array for each group of k-mers, each group signature array comprising the k-mers in each group that have the most increased frequency compared with the sibling k-mers; generating a signature map comprising each group signature array and at least one of the identifiers, the identifier of at least one parent group and the identifier of at least one child group; and outputting the signature map to be used to classify the sequence.

The invention relates to a Method for calculating a local extremum, preferably a local minimum, of a multidimensional function E(x1, x2, . . . , xn) which is interpretable as a function of potential energy with spatial coordinates (x1, x2, . . . , xn), using an iteration process based on a molecular dynamics based quenching method comprising the following steps: a) Calculating a trajectory X(ti) at discrete times ti=Σiδti starting from an assigned initial coordinate X(0) on basis of a gradient fk=δE / δxk with k=1, . . . ,n and a time derivatives of the coordinates vk=dxk / dt using fxk=mxk dvxk / dt in which mxk represent masses at the spatial coordinates and vxk=dxk / dt represents velocity b) Performing a molecular dynamics based quenching method for analysing said function E(x1, x2, . . . , xn) of existence of a local extremum, in case of reaching a local extremum abort processing and / or select a new initial coordinate and proceed further from step a) c) Calculating at each iteration time step δti F=(fxk) and (k=1, . . . ,n) representing a global force vector field acting in the spatial coordinates, V=(vxk) representing a velocity vector field, P=F·V representing power Setting V=(1−α)×V+α×(F / |F|)·|V|α is a dimensionless variable and amounts a given initial value αstart at first iteration time step In case of P<0: V is set to zero, α becomes αstart, δti will be reduced and return to step b) In case of P≧0: Analysing whether the number of conducted iteration steps since the last detected case of P<0 exceeds a given minimum number Nmin, in case of “no” returning to step b) and in case of “yes” increasing δti, decreasing α and return to step b).

The invention discloses a detection method and device of a planting seedling plant spacing and a hole seedling number of a rice transplanter. The method includes the steps: continually detecting working states and driving speed of the rice transplanter by a detecting and starting module; beginning to shoot a working area of the rice transplanter by a camera in real time when the rice transplanteris in a planting state; performing graying and binarization on RGB (red, green and blue) images of seedlings acquired by an embedded controller; extracting profile images of the seedlings, and calculating the planting seedling plant spacing and the hole seedling number of the rice transplanter according to a profile sudden change degree algorithm; broadcasting a current seedling plant spacing anda current hole seedling number by a voice reminding module; timely adjusting and controlling working parameters of the rice transplanter by a planting driver according to fed back information. Compared with the prior art, according to the technical scheme, the planting quality of the rice transplanter is automatically detected, and field working stability and field working reliability of the ricetransplanter are ensured.

Methods, systems and kits for the simultaneous or sequential analysis of one or more hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of thyroid hormones are disclosed including ionization of the thyroid hormones in the negative mode using an electrospray source.

A method for a three phase power flow analysis of an ungrounded power distribution system partitions a topology of the power distribution system into a mainline system and a set of tap systems, wherein the mainline system is formed by mainline buses connecting a swing bus and a set of constant active power and voltage magnitude (PV) buses, wherein the tap system is formed by one or many tap buses, such that a root bus of each tap system corresponds to a mainline bus. Next, the method determines voltages of the mainline buses based on a function of a total load of each tap system; and determines voltages of tap buses based on a voltage of the corresponding root bus of the mainline and load of each tap bus.

The present invention provides aza-BODIPY compound having formula 1 useful for detection of nitrite ions in an aqueous medium. The invention also provides a process for the preparation of aza-BODIPY compound having formula 1. For detecting nitrite ions, a dipstick device made by coating the assay powder of formula 1 in alumina over a thermoplastic or a glass solid support is used. The detection event can be monitored by noting the color change on the surface of the dipstick. Detection event by means of color change is selective for nitrite ions when compared to all other biologically important ions like SO42−, Cl−, HSO3−, CO32−, CH3COO−, NO3−, S2O32−, N3−.

A comprehensive model-based mathematical method for calibration of a gantry robot and a tool via a dual camera vision system may employ an automatic calibration process that includes: (i) Camera Internal and External Calibration (ULC and DLC), (ii) Camera Installation, (iii) Robot Workspace Calibration, and (iv) Tool Calibration. The method may provide an accurate, fast and affordable method for robot calibration which may significantly reduce the commissioning time, such that the time-consuming, cumbersome, conventional trial-and-error method may be reduced to a time-efficient automatic procedure with no human interference.

The present invention provides pyrrole end-capped bipyridine derivative having a general formula (1) useful for the detection of zinc ions in solution. The present invention also provides a process for the preparation of pyrrole end-capped bipyridine derivative having a general formula (1). The present invention further provides a dipstick device by coating the assay powder of formula (1) in alumina over a thermoplastic or a glass solid support. The detection event can be monitored by noting the color change and the intense fluorescence change on the surface of the dipstick. For zinc ions, in addition to the color change an intense fluorescence change from greenish-yellow to red is observed. Detection event by means of fluorescence change is selective for Zn2+ ions when compared with all other biologically important metal ions like Na+, K+, Ca2+; Mg2+, Cu2+. The present dipstick is reusable, and can conduct the analysis of different samples using same stick.

A method and system for volumetric quantification of local bone changes includes the steps of loading at least a first and second (cone-beam) computed tomography three dimensional image, registering the first three dimensional image and second three dimensional image to one coordinate system, selecting a region of interest in one of the first three dimensional image or the second three dimensional image. For the first three dimensional image and the second three dimensional image, segmenting the local bone within the region of interest by segmenting voxels related to air and / or soft tissues within the region of interest and attributing these voxels to an outside region, while the volume formed by the remaining volume represent the local bone or the local bone with soft tissue, and calculating of the volume of local bone in the first three dimensional image and the volume of local bone in the second three dimensional image and subtracting the first volume from the second volume and defining the difference as the local bone change or the change in local bone with soft tissue.

The present invention discloses a multi-level matter-element entropy weight-based congestion hotspot airspace sector recognition method and belongs to the air traffic management field. The method includes the following steps that: a three-level airspace sector congestion degree evaluation index system including a target level, a dimension level and an index level is established, the calculation or sampling method of indexes is established, evaluation indexes are calculated on the basis of evaluation sector sample selection, and index level threshold judgment is performed according to index numerical value distribution; a multi-level matter-element model is established, association degrees and index weights are calculated, a sector congestion dimension level is judged; and the calculation of a sector congestion dimension weight is determined on the basis of an entropy weight method, and a congestion hotspot airspace sector is recognized. According to the method of the invention, a sector congestion degree is comprehensively judged from a plurality of dimensions, technical support is provided for the research and development of control auxiliary decision-making system tools, and references are also provided for airspace planning, sector adjustment and the like.

The present invention describes the development of a end-capped bipyridine compound having formula A and the zinc complex having formula B. The assay having formula 1 can be used to estimate and quantify the amount of zinc ions by monitoring the fluorescence changes. The assay with formula 1 can be use to image and detect Zn2+ ions in MCF7 cell lines. The zinc complex of formula 2 and 4 can be used as a fluorescent sensor for cyanide anions using analyte replacement protocol. The assay with formula 2 is selective only to cyanide anions even in the presence of other competing anions. The assay with formula 3 having bright green solid state emission is used for the preparation of formula 4. The orange fluorescent powder of assay with formula 4 is used for the selective detection of CN− ions in aqueous solution.

The invention provides a method for quantitatively detecting saponin components in a Radix Ginseng Rubra and Radix Ophiopogonis injection. High performance liquid phase chromatography is adopted to simultaneously determine ginsenoside Rb1, Re and Rg1 and the content of at least one of the following saponin compounds: ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3. The method comprises the following steps: preparing a mixed solution reference substance solution, preparing a tested substance solution, setting liquid phase determination conditions, and sampling the above sample, wherein the chromatographic conditions of liquid phase determination conditions comprise an octadecyl silane bonded silica gel column, acetonitrile-water as a mobile phase to carry out gradient elution and an ultraviolet detector for detection. The method has the characteristics of good specificity, few interference, high sensitivity, good precision, good reappearance, stable tested substance solution, accurate determination, fastness, accuracy and short analysis time.

The present invention provides a method and an apparatus to analyze fast and accurately biological samples. The method includes prior mapping of the DNA chip with a CCD camera, addressed excitation of samples, and recording of fluorescence spectra via ultra-fast spectrometer.

The invention provides a laser precision galvanometer calibration accuracy detection method and a detection system. The method comprises the following steps: verification material is arranged on the boundary of at least one galvanometer scanning area; the verification material is at least arranged on the boundary in the two mutually-perpendicular directions; a plurality of partially-standard patterns are respectively processed on the verification material by laser, so that the partially-standard patterns are spliced to form at least one standard pattern; whether the integrity of the standard pattern after being spliced reaches a preset value is detected, therefore, the accuracy of a galvanometer can be judged. According to the laser precision galvanometer calibration accuracy detection method and the detection system, the detection and verification process for the calibrated galvanometer is simplified, the detection efficiency is improved, and the calibration accuracy of the galvanometer can be rapidly and accurately detected and verified.

The invention provides a kit for detecting an alpaca mycobacterium paratuberculosis antibody and a detection method thereof, belonging to the technical field of biology. The kit comprises mycobacterium paratuberculosis recombinant hed protein, positive control serum and negative control serum of alpaca paratuberculosis, an ELISA plate, coating liquid, confining liquid, PBST, staphylococcal protein A labeled by horseradish peroxidase (HRP-SPA), a raw material for preparing developing solution and stop solution. The kit of the invention introduces the high-expression mycobacterium paratuberculosis hed protein with strong specificity as a detection antigen for directly detecting the alpaca mycobacterium paratuberculosis antibody, thus having good specificity and sensitivity. The method for detecting the alpaca mycobacterium paratuberculosis antibody by the kit is simple, convenient, fast and accurate, thus being applicable to sample detection in batches.

The invention discloses a loop-mediated isothermal amplification detection method for detecting Fusarium oxysporum of Chinese wolfberry root rot, and detection primers and a verification method thereof. The detection primers are designed by adopting LAMP (Loop-mediated Isothermal Amplification) design software Primer Explorer V4, and comprise two outer primers F3 and B3 and two inner primers FIP and BIP. The invention has the following beneficial effect: By the detection method, the primers and the verification method, five Fusarium oxysporum from different geographical sources is yellow green, namely positive after LAMP detection, but the control and other pathogenic bacteria are orange, namely negative. The invention has the advantages of strong primer specificity, high detection sensitivity, high reaction speed, direct observation of results by naked eyes, high accuracy, difficulty in pollution, direct detection of suspected disease samples and the like. And meanwhile, comparison and judgment are performed through color development, so that the detection accuracy is greatly improved.

The invention discloses an on-line monitoring method of primary side shunt electric stealing of a CT (current transformer) of a 10kV special variable metering device, particularly relates to a method for searching for and locking electricity stealers by analyzing data which is acquired by a metering automation system. The on-line monitoring method is mainly characterized in that users who match a condition are treated as electricity stealing suspects by comparing whether power factor angle lag deviant values of split phases of time points to be inspected relative to interval time points are in the range of 40-65 DEG or not; and the electricity stealing suspects who cause abnormal line loss are treated as the electricity stealers by analyzing the data of line loss inflection point. The method for monitoring electricity stealing behaviors has very high accuracy; and an application of the method surely can bring great convenience to prevention of the electric larceny operation of utility companies.

Method for up-scaling a color image prior to performing subsequent processing on said color image, comprising the steps of converting the color image into multiple image layers distinguishable from each other and up-scaling at least one of said multiple image layers. The up-scaling is tuned towards the subsequent processing, for example luminance is upscaled at higher quality than chrominance. Further, a method for interpreting information present on digitally acquired documents, comprising the steps of: (i) determining a country; (ii) identifying a list of languages and character sets in use in said country; (iii) performing optical character recognition simultaneously using all languages and character sets of the list; (iv) performing field parsing to identify fields in the digitally acquired document on the basis of international as well as country-specific field recognition rules; (v) storing the recognized information according to the identified fields in a database.