160 results about "Intense fluorescence" patented technology

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention discloses a light-emitting super-structure surface capable of realizing incoherent fluorescence holography and multi-dimensional super-structure display and a preparation method thereof. The light-emitting super-structure surface is mainly composed of an optical waveguide structure layer, a micro-nano structure layer and a fluorescence emission layer. The optical waveguide structure layer comprises a substrate and a waveguide layer and is responsible for guiding fluorescence propagation; the micro-nano structure layer is located above the waveguide layer, is composed of nano bricks and can regulate and control light; the fluorescence emission layer is arranged above the micro-nano structure layer, contains quantum dots and is excited by the pump light to emit fluorescence. The metasurface can regulate and control the emergent angle, wavefront and emergent intensity of fluorescent light and pump light, the time-space coherence of the fluorescent light is enhanced through the waveguide layer, and phase modulation is carried out by using a method based on a circuitous phase. Based on the super-structure surface, through the steps of fluorescence excitation, coherence enhancement, phase modulation, diffraction calculation, hologram generation, multi-dimensional display and the like, incoherent fluorescence hologram and multi-dimensional super-structure display can be achieved, and the functions of fluorescence nanometer printing and multi-channel holographic display are covered.

The invention belongs to a fluorescence analysis and detection method of p-nitrophenol, and particularly relates to a fluorescent carbon quantum dot probe for detecting p-nitrophenol as well as a preparation method and application of the fluorescent carbon quantum dot probe. The specific preparation method of the fluorescent carbon quantum dot probe comprises the following steps: taking aminopyrimidine as a precursor, mixing aminopyrimidine with water, and reacting at 160-200 DEG C to obtain the fluorescent carbon quantum dot probe with the surface rich in hydroxyl, amino and carboxyl groups. The fluorescent carbon quantum dot probe prepared by the invention has a strong fluorescent signal, selectively responds to p-nitrophenol, shows a wider linear detection range and high anti-interference capability, and is suitable for complex environmental pollutant detection.

The invention belongs to the field of functional nano materials and optical imaging, and particularly relates to a gold nanocluster and a preparation method and application thereof. The surface of the gold nanocluster contains a sulfydryl ligand A and a sulfydryl ligand B, the sulfydryl ligand A is ATT, and the sulfydryl ligand B is a sulfydryl ligand containing arginine; fluorescence quantum efficiency and electron transfer efficiency are synergistically enhanced on the basis of interaction of hydrogen bonds in the gold nanoclusters, the ultra-small luminous gold nanoclusters protected by double ligands are synthesized, the electrochemical luminescence quantum efficiency of the ultra-small luminous gold nanoclusters is up to 13.0% and is 4.2 times of that of a standard compound terpyridyl ruthenium in the field of electrochemical luminescence, and the ultra-small luminous gold nanoclusters are high in electrochemical luminescence quantum efficiency. An electrochemical luminescence transition area can be narrowed by 10 times compared with a terpyridyl ruthenium imaging system, and zero-thickness ECL luminescent layer cell high-resolution imaging can be realized; good application prospects are realized in the fields of fluorescence detection, electrochemical luminescence detection, fluorescence imaging, electrochemical luminescence imaging and the like.

The invention discloses a three-dimensional fluorescent nano printing method capable of being monitored in situ in real time, which comprises the following steps: (1) preparing a photoresist which comprises the following components: a two-step absorption photoinitiator, a small molecule polymerization monomer, aggregation-induced emission dye molecules and a photoresist auxiliary reagent; (2) a continuous laser beam acts on the photoresist through a three-dimensional scanning system, a double-step absorption photoinitiator generates an active factor through a double-step absorption process, a photopolymerization reaction of a small molecule polymerization monomer is initiated, and three-dimensional nano printing is achieved; in the photopolymerization region, the doped aggregation-induced emission dye molecules enable the printing structure to present strong fluorescence and the non-polymerization region to present weak fluorescence; and (3) rapidly scanning the three-dimensional nano printing area, obtaining the fluorescence intensity distribution of the three-dimensional nano printing structure in real time, and tracking and exploring the dynamic process and error correction of the three-dimensional nano printing. According to the invention, ultrahigh-precision fluorescent pattern three-dimensional nano printing and real-time in-situ detection can be realized.

This invention provides a fluorescent labeling and conjugation method that exhibits enhanced signal intensity. [Solution] This disclosure relates to the field of fluorescent dyes, and more particularly to compositions and methods for increasing fluorescence signals and decreasing fluorescence quenching.

The utility model discloses a fluorescent strip for an aviation passenger cabin passage, which comprises a protective shell, a fluorescent strip and a connecting plate, the fluorescent strip is arranged in the protective shell, and the connecting plate is arranged at the bottom of the protective shell. According to the fluorescent strip for the aviation passenger cabin channel, the protective shell is hollow and matched with the fluorescent strip, the fluorescent strip can be protected against physical damage, the service life of the fluorescent strip is prolonged, the placement groove and the fluorescent strip are matched in size, a space is reserved, it is guaranteed that the fluorescent strip is stably installed and cannot be extruded to emit light, and the placement groove provides a stable installation position for the fluorescent strip; the dome design plays an important role, and the curved surface of the dome can diffuse light, so that fluorescence is uniformly distributed in the passenger cabin, and the visual range and definition are improved; meanwhile, the dome is resistant to pressure, can bear external treading force of people, disperse pressure, prevent the fluorescent strips from being damaged and maintain the emergency guiding function, and the reflectors on the two sides enhance fluorescent reflection, so that light rays are brighter and spread farther.

A fluorescence intensity enhancer containing a water-soluble compound having a melting point of 50° C. or higher, a fluorescence intensity enhancing method of a fluorescently labeled target biological substance using the fluorescence intensity enhancer, and a kit for fluorescence detection containing the fluorescence intensity enhancer.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The invention discloses workshop cutting fluid aerosol detection equipment based on fluorescent carbon dots and a detection method, the workshop cutting fluid aerosol detection equipment comprises at least one pulse breathing machine, the pulse breathing machine is detachably connected with a filter screen, and the filter screen is used for capturing aerosol particles; the at least one ultraviolet light source is used for irradiating and exciting the aerosol particles; the optical lens is used for shooting the irradiated and excited filter screen to form a filter screen picture; and the image processing module is used for processing the filter screen picture and obtaining an RGB value. The structure of aerosol detection equipment can be simplified, the manufacturing cost can be reduced, the aerosol detection efficiency can be improved, meanwhile, use is flexible and convenient, the occupied area of a workshop is reduced, and the detection method is simple in step; aerosol formed after the cutting fluid is atomized is marked by utilizing the strong fluorescence property excited by the fluorescent carbon dots under an ultraviolet light source, so that the detection cost is greatly reduced, and the detection method is simple and efficient.

The invention discloses octavinyl polyhedral oligomeric silsesquioxane coupled brominated tetraphenylethylene as well as a preparation method and application thereof, and belongs to the field of fluorescent probes. Firstly, the rigid cage-shaped structure of POSS obviously enhances the aggregation-induced emission effect of TPE, so that the probe shows strong fluorescence emission in organic solvents such as DMF (Dimethyl Formamide) and the like; secondly, the probe shows an obvious solvent discoloration characteristic and shows adjustable optical response in different solvents, and meanwhile, the probe is endowed with excellent chemical stability by a POSS (Polyhedral Oligomeric Silsesquioxane) skeleton; thirdly, the probe has high selectivity and sensitivity recognition capability on heavy metal ions such as Fe < 2 + > and anions such as CN <->, the detection limit can reach 10 <-3 > mol / L, and visual detection can be realized through fluorescence change; in addition, a simple and convenient Heck coupling method is adopted for synthesis, the reaction condition is mild, the yield is up to 85%, and purification and large-scale production are easy.

The present application belongs to the technical field of food safety detection, and discloses a rapid Raman detection system and method for illegal additives in food; the illegal additives are enriched through dispersed liquid-liquid microextraction pretreatment, fluorescence emission spectrum data is obtained under low-power broadband excitation, and a fluorescence intensity gradient analysis is used to identify a fluorescence saturation risk section. The target spectrum range is divided into weak fluorescence and strong fluorescence collection windows arranged alternately, and differentiated collection parameters and processing strategies are used respectively; the offset excitation technology is used for the strong fluorescence window to obtain a difference spectrum; and the weak fluorescence window is used for accurate baseline deduction to generate a purified spectrum. Through splicing and connection, a complete composite purified spectrum is formed, and Raman true peaks and fluorescence false peaks are distinguished by combining peak identification and peak shape symmetry analysis. Finally, template matching is carried out based on peak position combination and intensity ratio characteristics, so that the illegal additives are accurately identified. The present application improves the accuracy, sensitivity and efficiency of food safety rapid screening.

The invention relates to a samarium-doped carbon quantum dot as well as a preparation method and application thereof, and belongs to the technical field of nano material synthesis, the preparation method comprises the following steps: carrying out ultrasonic dispersion on 0.01-0.03 g of p-phenylenediamine, 0.05-0.10 g of N-(4-aminophenyl) acetamide and 0.01-0.02 g of samarium chloride (III) hexahydrate in 15-25 mL of ultrapure water to form a mixed solution, carrying out hydrothermal reaction at 160-200 DEG C for 8-12 hours, filtering, washing and drying to obtain the samarium-doped carbon quantum dot. High-speed centrifugation, millipore filtration membrane filtration, 500Da molecular weight interception dialysis and freeze drying processes are sequentially adopted to realize efficient doping of the samarium element and precise purification of the product. The method is easy and convenient to operate, the product uniformity is excellent, and the prepared samarium-doped carbon quantum dots have the strong fluorescence characteristic and chemical stability and can be widely applied to the fields of biomedical imaging, photocatalytic materials and photo-thermal anticancer.

The invention belongs to the technical field of fluorescent probes, and relates to a preparation method and properties of two pH, viscosity and polarity three-response near-infrared fluorescent probes. The probe PODI and the probe PODB have the strongest fluorescence in an alkaline condition under excitation of 390nm, and the fluorescence is weakened when the acidity is enhanced; when excitation is carried out at 600nm, the fluorescence intensity is enhanced along with the increase of the glycerol content, and the viscosity change in acidic and neutral systems can be detected. The two probes present fluorescence response to polarity change: polarity-enhanced fluorescence rises under excitation of 390nm, and polarity-reduced fluorescence rises under excitation of 600nm. The preparation method is simple, high in yield and suitable for industrial production.

Described herein are methods and systems for training image to image translation machine-learning models, wherein the image to image translation machine-learning models are trained using unpaired images. The trained image to image translation machine-learning models can be used to digitally identify markers of a fluorescent image depicting a plurality of markers in a single color channel from a single fluorophore and / or enhance a fluorescence image.

A vibrationally enhanced fluorescence microscopy system includes a narrowband pulsed mid-infrared laser configured to excite selected chemical bond vibrations in a sample. A continuous-wave visible laser is coaligned with the mid-infrared laser and configured to induce fluorescence from at least one fluorescent reporter bound to the sample. An external pulse generator temporally synchronizes emission of the mid-infrared pulses with emission of the visible laser. A reflective objective is arranged to deliver the mid-infrared pulses to a focal plane within the sample. A water-immersion objective is coaxially aligned with the reflective objective and arranged to collect the fluorescence from the focal plane. A photon-counting detector is coupled to receive the collected fluorescence. Control electronics are configured to output a vibrational-contrast signal derived from counts produced by the photon-counting detector.

Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.

The present invention discloses a method for preparing a metal corrosion inhibitor component, its products, and applications. The method involves reacting a halogenated tetraphenylethylene with aminopolyethylene glycol or aminoethanol to produce a metal corrosion inhibitor component. The component exhibits excellent corrosion inhibition performance and is easily detectable in corrosive media. Furthermore, the component emits strong fluorescence upon adsorption onto metal surfaces, enabling visual detection.

The invention relates to a breast cancer diagnosis probe, in particular to a fluorescence / magnetic resonance enhanced bimodal breast cancer imaging polypeptide probe as well as a preparation method and application thereof. The polypeptide probe is prepared from a fluorescence imaging unit, a magnetic resonance imaging unit, a self-assembly imaging unit and a TROP-2 targeted recognition unit. According to the invention, the polypeptide probe for enhancing fluorescence / magnetic resonance signals through in-situ self-assembly is constructed, and the polypeptide probe synthesized through modular optimization can realize in-situ self-assembly mediated by high-expression TROP-2 protein of triple negative breast cancer cells; self-assembly along with fluorescence and magnetic resonance unit aggregation can significantly enhance fluorescence and magnetic resonance bimodal imaging signals of triple-negative breast cancer tissues, improve imaging sensitivity and enhance triple-negative breast cancer imaging effects.

Presented herein are compositions, systems, and methods related to optical substrates that simultaneous (1) enhance a fluorescence signal emitted by a fluorophore and (2) enhance “contrast” signal that comprises scattered signal intensity over substrate reflectivity at a non-fluorescent wavelength. In certain embodiments, the optical substrate comprises a thin, transparent, dielectric layer. In alternative embodiments, the optical substrate comprises a stack of thin, transparent dielectric layers, for example, that is designed for both specific scattering enhancement and fluorescence enhancement.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The invention provides a covalent organic framework spaced plasma enhanced fluorescent nano composite material as well as a preparation method and application thereof, and relates to the technical field of nano materials and biological detection. The material sequentially comprises a SiO2 nanosphere core, an Au nano shell layer, a covalent organic framework layer and a carboxylated quantum dot layer from inside to outside, wherein the covalent organic framework layer comprises a Schiff base type covalent organic framework formed by connecting 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-dimethoxy terephthalaldehyde through a carbon-nitrogen double bond; the thickness of the covalent organic framework layer is 3 to 24 nm; wherein the carboxylated quantum dot layer is prepared from CdSe / ZnS-COOH particles. The rigid spacing material which is stable in structure and capable of accurately regulating and controlling the thickness is constructed and is used for constructing a stable and controllable spatial distance between the metal nanoparticles and the fluorophor, so that plasma enhanced fluorescence is realized, and the immunochromatography detection performance is improved.

The invention discloses a method for detecting a quantum state of a single rare earth ion. The method is based on photonic crystal microcavity enhancement and microwave-light pulse collaborative excitation. A one-dimensional photonic crystal microcavity is arranged in a rare earth ion-doped yttrium silicate crystal, a defect is introduced to enable a resonant wavelength to be matched with a target transition wavelength, and a Purcell effect is utilized to shorten the lifetime of an excited state, improve a target transition branching ratio and enhance fluorescence radiation. Rare earth ions are excited by comprehensively using microwave pulse and light pulse, the quantum state is preprocessed by the microwave pulse, then fluorescence photons are excited and collected by the light pulse, the quantum state is judged according to the change of fluorescence intensity along with integral time, and if the quantum state is obviously increased, the quantum state is the state. According to the detection method provided by the invention, the problems of weak signal and low efficiency of the traditional detection are solved, and an efficient scheme is provided for quantum state reading.

The invention provides a PCR chip, a manufacturing method thereof and a PCR chip detection method. The PCR chip comprises a substrate layer and a plurality of reaction units which are arranged on the substrate layer in an array mode, and each reaction unit comprises a reaction chamber and a plurality of silicon-based nano columns which are arranged in the reaction chamber in an array mode. In the invention, by utilizing the characteristic that the nano-scale metasurface array based on the silicon-based material can enhance the fluorescence signal, the plurality of silicon-based nano columns arranged in the array are added in the reaction unit and are used for enhancing the PCR reaction fluorescence signal and improving the resolution ratio of the fluorescence intensity, so that the purposes of improving the detection sensitivity of the PCR chip and reducing the detection error are achieved.

According to the protein detection method and the detection preparation provided by the invention, efficient enrichment of target protein is realized through the mesoporous silica-graphene oxide composite nano-porous material, and specific immunocapture is completed in combination with the magnetic nanoparticles modified by the targeted antibody; and the micro-fluidic chip of the three-dimensional hydrogel antibody array is used for realizing single molecule separation. An enzyme-labeled secondary antibody-gold nanorod conjugate is adopted for signal amplification, a fluorescence signal is enhanced through a plasma resonance effect, background noise is corrected in combination with a convolutional neural network algorithm, and the detection reliability is remarkably improved; experiments show that the detection limit of the method is as low as 0.12 pg / mL, the linear range covers 0.5-100,000 pg / mL, the single cell detection error is + / -7.8%, the batch repeatability (CV = 4.2%) is realized, the non-specific adsorption rate is only 1.1%, and the detection time is shortened to 1.2 hours. The detection method is suitable for ultra-sensitive detection of tumor markers, and especially has clinical application potential in CTCs in-situ analysis.

The invention provides a system for ctDNA double-target detection based on a DNA functional nanomaterial and hydrogel and application, and relates to the technical field of biomedical diagnostic analysis, the system is used for executing the following analysis method, and the analysis method is to realize target specific recognition and trigger cascade signal amplification through the synergistic effect of DNA hydrogel and DNA coated Cu < 2 + > nanospheres. G-quadruplex exposed by DNA hydrogel can be efficiently combined with thioflavin T to generate enhanced fluorescence, and Cu < 2 + > released by DNA coated Cu < 2 + > nanospheres realizes dual-signal output by quenching quantum dot emission. Based on an advanced nano material and a signal amplification strategy, high-sensitivity, rapid and homogeneous ctDNA double-target detection is realized, sample separation operation is not needed in the whole detection process, and the almole-level detection limit can be reached within 40 minutes.

The present application relates to a kind of high-throughput staphylococcus aureus enterotoxin microfluidic chip detection method and application based on chain quantity amplification enhanced fluorescence.The microfluidic chip is provided with fluorescence sensor, and the fluorescence sensor includes identification probe and signal probe;The identification probe is composed of magnetic nanoparticle, nucleic acid aptamer H1 of target to be measured and target aptamer complementary chain H2, and the signal probe is composed of nanomaterial with signal amplification function and three oligonucleotides H3, H4, H5.The present application combines MNPs-ssDNA-AuNPs sandwich structure system and microfluidic chip, realizes detection signal amplification using the excellent superparamagnetic property of MNPs and the high loading capacity of AuNPs, constructs high-throughput, high-sensitivity fluorescence sensing technology for SEA, SEB and SEC1 detection, simplifies experimental process, and builds the rapid, efficient detection platform of multiple pollutants in food.

The invention belongs to the technical field of contraceptives, and particularly relates to a condom with a fluorescence function and a preparation method of the condom. The condom with the fluorescent function sequentially comprises a base film, a fluorescent layer and a protective layer from inside to outside, the nano fluorescent powder adopted in the fluorescent layer takes Y (NO3) 3.6 H2O and Eu (NO3) 3.6 H2O as main raw materials, Eu < 3 + > is used as a light-emitting center and is stably wrapped by a Y < 3 + > matrix, and a foundation is laid for strong fluorescence emission; amino groups are introduced to the surface of the nanopowder through amination modification of KH-550, acid-base interaction is formed between the amino groups and carboxyl of polyacrylic acid, the surfaces of particles are firmly coated with polyacrylic acid, and a double-coating structure is achieved; the fluorescent powder is uniformly distributed in the latex through the pre-dispersion treatment of the nano fluorescent powder and the dispersing agent and the layered dipping process, so that the mechanical stability and the fluorescent durability of the condom are bidirectionally improved.

The present application relates to a kind of metal nanoparticle-based plasmonic enhanced fluorescence multilayer film optical structure and its preparation method and application, the multilayer film optical structure is by with self-assembly metal nanoparticle as enhancement substrate, to be measured fluorescent probe molecule is placed on the surface of the enhancement substrate and obtains.The present application is by the surface plasmon resonance of self-assembly metal nanoparticle, while effectively inhibiting fluorescent probe molecule photobleaching, greatly improves the sensitivity of fluorescence trace gas sensing, the service life of organic probe fluorescence and gas sensor reliability;Compared with the prior art glass substrate fluorescent film technology, the performance of the present application is comprehensively improved, and has good market application prospect.

The invention provides a probe, a kit and a detection method for detecting salmonella, and belongs to the technical field of pathogenic bacterium detection. The research provides an unmarked fluorescent biosensing platform for high-sensitivity detection of salmonella. A target sequence is introduced into a catalytic core sequence of Aurora DNAzyme, so that a signal probe E-Aurora is constructed. The E-Aurora can be combined with a substrate 4-methylumbelliferone phosphate disodium salt to generate a strong fluorescent compound 4-MU. When salmonella exists, PfAgo is activated and specifically cuts the E-Aurora probe, so that the structural integrity of E-Aurora is destroyed and the catalytic ability of E-Aurora is lost, and the generation of a fluorescence signal is hindered. The biosensor realizes ultra-high sensitivity detection of salmonella, the limit of detection (LOD) is as low as 1 CFU / mL, and an ideal recovery rate (80-120%) is obtained in actual sample detection. The detection method disclosed by the invention is simple to operate and wide in application range, and can be used for determining trace salmonella in food.