111 results about "Intense fluorescence" patented technology

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention belongs to the field of functional nano materials and optical imaging, and particularly relates to a gold nanocluster and a preparation method and application thereof. The surface of the gold nanocluster contains a sulfydryl ligand A and a sulfydryl ligand B, the sulfydryl ligand A is ATT, and the sulfydryl ligand B is a sulfydryl ligand containing arginine; fluorescence quantum efficiency and electron transfer efficiency are synergistically enhanced on the basis of interaction of hydrogen bonds in the gold nanoclusters, the ultra-small luminous gold nanoclusters protected by double ligands are synthesized, the electrochemical luminescence quantum efficiency of the ultra-small luminous gold nanoclusters is up to 13.0% and is 4.2 times of that of a standard compound terpyridyl ruthenium in the field of electrochemical luminescence, and the ultra-small luminous gold nanoclusters are high in electrochemical luminescence quantum efficiency. An electrochemical luminescence transition area can be narrowed by 10 times compared with a terpyridyl ruthenium imaging system, and zero-thickness ECL luminescent layer cell high-resolution imaging can be realized; good application prospects are realized in the fields of fluorescence detection, electrochemical luminescence detection, fluorescence imaging, electrochemical luminescence imaging and the like.

This invention provides a fluorescent labeling and conjugation method that exhibits enhanced signal intensity. [Solution] This disclosure relates to the field of fluorescent dyes, and more particularly to compositions and methods for increasing fluorescence signals and decreasing fluorescence quenching.

The utility model discloses a fluorescent strip for an aviation passenger cabin passage, which comprises a protective shell, a fluorescent strip and a connecting plate, the fluorescent strip is arranged in the protective shell, and the connecting plate is arranged at the bottom of the protective shell. According to the fluorescent strip for the aviation passenger cabin channel, the protective shell is hollow and matched with the fluorescent strip, the fluorescent strip can be protected against physical damage, the service life of the fluorescent strip is prolonged, the placement groove and the fluorescent strip are matched in size, a space is reserved, it is guaranteed that the fluorescent strip is stably installed and cannot be extruded to emit light, and the placement groove provides a stable installation position for the fluorescent strip; the dome design plays an important role, and the curved surface of the dome can diffuse light, so that fluorescence is uniformly distributed in the passenger cabin, and the visual range and definition are improved; meanwhile, the dome is resistant to pressure, can bear external treading force of people, disperse pressure, prevent the fluorescent strips from being damaged and maintain the emergency guiding function, and the reflectors on the two sides enhance fluorescent reflection, so that light rays are brighter and spread farther.

A fluorescence intensity enhancer containing a water-soluble compound having a melting point of 50° C. or higher, a fluorescence intensity enhancing method of a fluorescently labeled target biological substance using the fluorescence intensity enhancer, and a kit for fluorescence detection containing the fluorescence intensity enhancer.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The present application belongs to the technical field of food safety detection, and discloses a rapid Raman detection system and method for illegal additives in food; the illegal additives are enriched through dispersed liquid-liquid microextraction pretreatment, fluorescence emission spectrum data is obtained under low-power broadband excitation, and a fluorescence intensity gradient analysis is used to identify a fluorescence saturation risk section. The target spectrum range is divided into weak fluorescence and strong fluorescence collection windows arranged alternately, and differentiated collection parameters and processing strategies are used respectively; the offset excitation technology is used for the strong fluorescence window to obtain a difference spectrum; and the weak fluorescence window is used for accurate baseline deduction to generate a purified spectrum. Through splicing and connection, a complete composite purified spectrum is formed, and Raman true peaks and fluorescence false peaks are distinguished by combining peak identification and peak shape symmetry analysis. Finally, template matching is carried out based on peak position combination and intensity ratio characteristics, so that the illegal additives are accurately identified. The present application improves the accuracy, sensitivity and efficiency of food safety rapid screening.

Described herein are methods and systems for training image to image translation machine-learning models, wherein the image to image translation machine-learning models are trained using unpaired images. The trained image to image translation machine-learning models can be used to digitally identify markers of a fluorescent image depicting a plurality of markers in a single color channel from a single fluorophore and / or enhance a fluorescence image.

A vibrationally enhanced fluorescence microscopy system includes a narrowband pulsed mid-infrared laser configured to excite selected chemical bond vibrations in a sample. A continuous-wave visible laser is coaligned with the mid-infrared laser and configured to induce fluorescence from at least one fluorescent reporter bound to the sample. An external pulse generator temporally synchronizes emission of the mid-infrared pulses with emission of the visible laser. A reflective objective is arranged to deliver the mid-infrared pulses to a focal plane within the sample. A water-immersion objective is coaxially aligned with the reflective objective and arranged to collect the fluorescence from the focal plane. A photon-counting detector is coupled to receive the collected fluorescence. Control electronics are configured to output a vibrational-contrast signal derived from counts produced by the photon-counting detector.

Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.

The invention relates to a breast cancer diagnosis probe, in particular to a fluorescence / magnetic resonance enhanced bimodal breast cancer imaging polypeptide probe as well as a preparation method and application thereof. The polypeptide probe is prepared from a fluorescence imaging unit, a magnetic resonance imaging unit, a self-assembly imaging unit and a TROP-2 targeted recognition unit. According to the invention, the polypeptide probe for enhancing fluorescence / magnetic resonance signals through in-situ self-assembly is constructed, and the polypeptide probe synthesized through modular optimization can realize in-situ self-assembly mediated by high-expression TROP-2 protein of triple negative breast cancer cells; self-assembly along with fluorescence and magnetic resonance unit aggregation can significantly enhance fluorescence and magnetic resonance bimodal imaging signals of triple-negative breast cancer tissues, improve imaging sensitivity and enhance triple-negative breast cancer imaging effects.

Presented herein are compositions, systems, and methods related to optical substrates that simultaneous (1) enhance a fluorescence signal emitted by a fluorophore and (2) enhance “contrast” signal that comprises scattered signal intensity over substrate reflectivity at a non-fluorescent wavelength. In certain embodiments, the optical substrate comprises a thin, transparent, dielectric layer. In alternative embodiments, the optical substrate comprises a stack of thin, transparent dielectric layers, for example, that is designed for both specific scattering enhancement and fluorescence enhancement.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The invention provides a covalent organic framework spaced plasma enhanced fluorescent nano composite material as well as a preparation method and application thereof, and relates to the technical field of nano materials and biological detection. The material sequentially comprises a SiO2 nanosphere core, an Au nano shell layer, a covalent organic framework layer and a carboxylated quantum dot layer from inside to outside, wherein the covalent organic framework layer comprises a Schiff base type covalent organic framework formed by connecting 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-dimethoxy terephthalaldehyde through a carbon-nitrogen double bond; the thickness of the covalent organic framework layer is 3 to 24 nm; wherein the carboxylated quantum dot layer is prepared from CdSe / ZnS-COOH particles. The rigid spacing material which is stable in structure and capable of accurately regulating and controlling the thickness is constructed and is used for constructing a stable and controllable spatial distance between the metal nanoparticles and the fluorophor, so that plasma enhanced fluorescence is realized, and the immunochromatography detection performance is improved.

The invention discloses a method for detecting a quantum state of a single rare earth ion. The method is based on photonic crystal microcavity enhancement and microwave-light pulse collaborative excitation. A one-dimensional photonic crystal microcavity is arranged in a rare earth ion-doped yttrium silicate crystal, a defect is introduced to enable a resonant wavelength to be matched with a target transition wavelength, and a Purcell effect is utilized to shorten the lifetime of an excited state, improve a target transition branching ratio and enhance fluorescence radiation. Rare earth ions are excited by comprehensively using microwave pulse and light pulse, the quantum state is preprocessed by the microwave pulse, then fluorescence photons are excited and collected by the light pulse, the quantum state is judged according to the change of fluorescence intensity along with integral time, and if the quantum state is obviously increased, the quantum state is the state. According to the detection method provided by the invention, the problems of weak signal and low efficiency of the traditional detection are solved, and an efficient scheme is provided for quantum state reading.

The invention provides a PCR chip, a manufacturing method thereof and a PCR chip detection method. The PCR chip comprises a substrate layer and a plurality of reaction units which are arranged on the substrate layer in an array mode, and each reaction unit comprises a reaction chamber and a plurality of silicon-based nano columns which are arranged in the reaction chamber in an array mode. In the invention, by utilizing the characteristic that the nano-scale metasurface array based on the silicon-based material can enhance the fluorescence signal, the plurality of silicon-based nano columns arranged in the array are added in the reaction unit and are used for enhancing the PCR reaction fluorescence signal and improving the resolution ratio of the fluorescence intensity, so that the purposes of improving the detection sensitivity of the PCR chip and reducing the detection error are achieved.

The invention provides a system for ctDNA double-target detection based on a DNA functional nanomaterial and hydrogel and application, and relates to the technical field of biomedical diagnostic analysis, the system is used for executing the following analysis method, and the analysis method is to realize target specific recognition and trigger cascade signal amplification through the synergistic effect of DNA hydrogel and DNA coated Cu < 2 + > nanospheres. G-quadruplex exposed by DNA hydrogel can be efficiently combined with thioflavin T to generate enhanced fluorescence, and Cu < 2 + > released by DNA coated Cu < 2 + > nanospheres realizes dual-signal output by quenching quantum dot emission. Based on an advanced nano material and a signal amplification strategy, high-sensitivity, rapid and homogeneous ctDNA double-target detection is realized, sample separation operation is not needed in the whole detection process, and the almole-level detection limit can be reached within 40 minutes.

The present application relates to a kind of high-throughput staphylococcus aureus enterotoxin microfluidic chip detection method and application based on chain quantity amplification enhanced fluorescence.The microfluidic chip is provided with fluorescence sensor, and the fluorescence sensor includes identification probe and signal probe;The identification probe is composed of magnetic nanoparticle, nucleic acid aptamer H1 of target to be measured and target aptamer complementary chain H2, and the signal probe is composed of nanomaterial with signal amplification function and three oligonucleotides H3, H4, H5.The present application combines MNPs-ssDNA-AuNPs sandwich structure system and microfluidic chip, realizes detection signal amplification using the excellent superparamagnetic property of MNPs and the high loading capacity of AuNPs, constructs high-throughput, high-sensitivity fluorescence sensing technology for SEA, SEB and SEC1 detection, simplifies experimental process, and builds the rapid, efficient detection platform of multiple pollutants in food.

The invention belongs to the technical field of contraceptives, and particularly relates to a condom with a fluorescence function and a preparation method of the condom. The condom with the fluorescent function sequentially comprises a base film, a fluorescent layer and a protective layer from inside to outside, the nano fluorescent powder adopted in the fluorescent layer takes Y (NO3) 3.6 H2O and Eu (NO3) 3.6 H2O as main raw materials, Eu < 3 + > is used as a light-emitting center and is stably wrapped by a Y < 3 + > matrix, and a foundation is laid for strong fluorescence emission; amino groups are introduced to the surface of the nanopowder through amination modification of KH-550, acid-base interaction is formed between the amino groups and carboxyl of polyacrylic acid, the surfaces of particles are firmly coated with polyacrylic acid, and a double-coating structure is achieved; the fluorescent powder is uniformly distributed in the latex through the pre-dispersion treatment of the nano fluorescent powder and the dispersing agent and the layered dipping process, so that the mechanical stability and the fluorescent durability of the condom are bidirectionally improved.

The present application relates to a kind of metal nanoparticle-based plasmonic enhanced fluorescence multilayer film optical structure and its preparation method and application, the multilayer film optical structure is by with self-assembly metal nanoparticle as enhancement substrate, to be measured fluorescent probe molecule is placed on the surface of the enhancement substrate and obtains.The present application is by the surface plasmon resonance of self-assembly metal nanoparticle, while effectively inhibiting fluorescent probe molecule photobleaching, greatly improves the sensitivity of fluorescence trace gas sensing, the service life of organic probe fluorescence and gas sensor reliability;Compared with the prior art glass substrate fluorescent film technology, the performance of the present application is comprehensively improved, and has good market application prospect.

The invention provides a probe, a kit and a detection method for detecting salmonella, and belongs to the technical field of pathogenic bacterium detection. The research provides an unmarked fluorescent biosensing platform for high-sensitivity detection of salmonella. A target sequence is introduced into a catalytic core sequence of Aurora DNAzyme, so that a signal probe E-Aurora is constructed. The E-Aurora can be combined with a substrate 4-methylumbelliferone phosphate disodium salt to generate a strong fluorescent compound 4-MU. When salmonella exists, PfAgo is activated and specifically cuts the E-Aurora probe, so that the structural integrity of E-Aurora is destroyed and the catalytic ability of E-Aurora is lost, and the generation of a fluorescence signal is hindered. The biosensor realizes ultra-high sensitivity detection of salmonella, the limit of detection (LOD) is as low as 1 CFU / mL, and an ideal recovery rate (80-120%) is obtained in actual sample detection. The detection method disclosed by the invention is simple to operate and wide in application range, and can be used for determining trace salmonella in food.

The invention discloses an AIE fluorescent probe as well as a preparation method and application thereof, and the structural formula of the probe is shown in the specification. According to the fluorescent probe disclosed by the invention, a fluorescent signal is remarkably enhanced through the aggregation-induced emission effect of a triphenylethylene skeleton, the application effect of ONOO-in the biological field is effectively improved, and the adaptability to multiple task types is realized.

The invention relates to a quick detection method of pyrethroid, belongs to the technical field of fluorescent probes, and discloses a plurality of micromolecular fluorescent probes based on coumarin derivatives as well as a preparation method and application of the micromolecular fluorescent probes. The invention provides a plurality of micromolecular fluorescent probes DAC, DBC, DCC, DDC, DEC, DFC, DGC, DHC and DIC for targeted detection of pyrethroid pesticides based on coumarin derivatives, when the probes act with the pyrethroid pesticides, the fluorescence becomes strong from weak, and the fluorescence intensity is significantly enhanced in a certain range. Fluorescence performance tests show that the coumarin derivative-based micromolecular fluorescent probe pyrethroid pesticide has good linear response, and has good stability and strong anti-interference capability under different pH, temperature, salt concentration and illumination conditions. According to the actual sample detection principle, the pyrethroid pesticide in crops is detected, the detection limit is as low as 9.7 pM, the adding standard recovery rate is 99%-102%, and the results are consistent with those of a liquid chromatography method and an on-site rapid detection method. Therefore, the fluorescent probe provided by the invention has a good application prospect.

The invention discloses a high-adhesion anode fluorescent screen and a manufacturing method thereof, and relates to the technical field of low-light-level image intensifier core component manufacturing, the high-adhesion anode fluorescent screen comprises a fluorescent screen, the fluorescent screen is made of glass or a sapphire substrate, the fluorescent screen is provided with a micropore array structure, the micropore array structure comprises a plurality of micropores arranged in an array, the aperture of each micropore is 5-10 [mu] m, and the diameter of each micropore is 5-10 [mu] m. The hole depth is 3-5 microns, the hole pitch is 1-2 microns, fluorescent powder is filled in each micropore, and the fluorescent powder is made of P43 or P45 fluorescent powder. According to the invention, the micropore array can form an anchoring structure, the fluorescent powder slurry is coated, the holes are filled with the particles and the binder, and the fluorescent powder layer and the substrate are locked; the stripping tendency of the fluorescent powder layer caused by thermal expansion and cold contraction, vibration or external stress is resisted through geometric constraint, and interaction force is generated with internal stress of the fluorescent powder layer, so that adhesion is enhanced; a narrow-band efficient fluorescent material is selected to cooperate with the micropore light regulation effect, the efficient light-emitting characteristic of the fluorescent powder is enhanced, a mature manufacturing process combination is combined, and breakthrough improvement of the performance of the fluorescent screen is achieved.

This invention relates to the field of optical sensing and imaging detection technology, specifically to a triphenylene-oxanthracene derivative, its preparation method, and a fluorescent probe for viscosity detection. The triphenylene-oxanthracene derivative has the general formula shown in Formula I. Compared to Rhodamine B, the synthesized triphenylene-oxanthracene derivative exhibits a larger Stokes shift. Furthermore, the fluorescence intensity of the triphenylene-oxanthracene derivative increases with increasing cell viscosity. In addition, the triphenylene-oxanthracene derivative can be further used to detect mitochondrial viscosity, exhibiting good mitochondrial targeting. In summary, the probe improves the Stokes shift, has a wide optical response pH range, and possesses excellent mitochondrial targeting.

The invention discloses a nopinic alkyl carbazole fluorescent probe capable of realizing double detection on viscosity and pH as well as a preparation method and application of the nopinic alkyl carbazole fluorescent probe. According to the preparation method, TC-AP-F is taken as a raw material, and the TC-AP-F and 1-(2-hydroxyethyl)-2, 3, 3-trimethyl-3H-indolinium iodide are subjected to a condensation reaction, so that the fluorescent probe TC-AP-I is prepared. The probe can specifically recognize viscosity and pH. Under the irradiation of ultraviolet light with the excitation wavelength of 365 nm, the probe solution does not emit fluorescence when the pH is less than 7; when the pH value is greater than 7, blue fluorescence is emitted, the fluorescence intensity at the emission wavelength of 455nm is gradually enhanced along with the increase of the pH value, but the fluorescence intensity is gradually weakened along with the increase of the viscosity, and the detection range of the pH value is 7-10; under the irradiation of ultraviolet light with the excitation wavelength of 480 nm, the fluorescence intensity of a probe solution is only influenced by the viscosity, the red fluorescence intensity at the emission wavelength of 655 nm is gradually enhanced along with the increase of the viscosity, the influence of the pH fluorescence intensity is very small, and the detection range of the viscosity is 0.9 cP to 1011 cP. The probe has the advantages of being simple and convenient to synthesize, good in selectivity, high in sensitivity, good in biocompatibility, low in toxicity and the like, and has a good application prospect.

The application discloses a fluorescent material for rapidly detecting cesium ions in water, and a preparation method thereof is as follows: first, synthesizing carbon quantum dots (NCD) with positive charges on the surface through a hydrothermal method, and then preparing a 0.1-100 mg.mL ‑1 0.02-100 mg.mL ‑1 aqueous solution of a heteropoly acid. The surface of the formed composite material presents negative charges and the original strong fluorescence effect is inhibited to be weak fluorescence. When the water contains cesium ions capable of forming an association with the heteropoly acid anions, the heteropoly acid anions are "stolen" by the cesium ions, and then the strong fluorescence performance of the material is recovered, indicating that the water is polluted by containing the cesium ions. The fluorescent material is high in sensitivity, low in detection limit, rapid in detection and simple in operation, and is especially suitable for monitoring of cesium ion pollution in seawater.

The invention discloses a three-dimensional fluorescence characteristic enhancement and antibiotic identification method based on absorption correction. The method comprises the following steps: firstly, carrying out preliminary scattering removal and internal filter effect correction on original excitation-emission matrix (EEM) data; then, accurately measuring an absorption spectrum of a water sample, and carrying out refined absorption correction on EEM data based on a fluorescence quantum yield theory, so that a fluorescence signal is remarkably enhanced, and overlapped fluorescence peaks are effectively separated; and finally, performing parallel factor analysis (PARAFAC) on the enhanced EEM data, and performing correlation coefficient matching with a standard spectrum database to realize accurate qualitative identification and high-precision quantitative analysis on different antibiotics in the water sample. According to the method, the real fluorescent fingerprints of the antibiotics can be recovered to the maximum extent, and the sensitivity, accuracy and reliability of antibiotic pollution detection are remarkably improved.

The invention provides an aggregation-induced emission (AIE) ionic probe (E)-4-(2-(5-(4-(diphenylamino) phenyl) thiophene-2-yl) vinyl)-1-(4-sulfanilamide acyl benzyl) quinoline-1-onium hexafluorophosphoric acid (V) salt of a targeted golf apparatus, namely a fluorescent probe TTQ-GA, a preparation method of the fluorescent probe TTQ-GA and application of the fluorescent probe TTQ-GA in the aspect of detection. The benzenesulfonamide group of the probe can be selectively combined with a Golgi apparatus protein cyclooxygenase 2 (COX-2) inhibitor SC-558 to realize Golgi apparatus targeting; due to the hydrophobic triphenylamine part and the hydrophilic quinoline cation part of the probe, the probe has amphipathy; the probe does not emit fluorescence in an aqueous solution, and can emit strong fluorescence when molecular movement is limited and excited, so that no-wash marking of the Golgi apparatus is realized; the probe generates a large amount of ROS under light excitation, promotes local lipid peroxidation increase of Golgi apparatus, and promotes cancer cell apoptosis.

The invention belongs to the technical field of environment detection, and particularly relates to a method for detecting Cu < 2 + > in a water body based on a biosynthetic cadmium sulfide quantum dot fluorescent probe. Cadmium sulfide quantum dots biosynthesized by an extracellular polymeric substance (EPS) are used as a fluorescent probe for detecting Cu < 2 + >, specifically, EPS is generated through carbon source stress / induction of sulfur vibrio desulfurized subspecies, the cadmium sulfide quantum dots with strong fluorescence are synthesized in the EPS, then the cadmium sulfide quantum dots and a buffer solution are mixed to prepare a fluorescent probe solution, and the fluorescent probe is used for detecting Cu < 2 + >. The obtained cadmium sulfide quantum dot fluorescent probe is used for detecting Cu < 2 + > in a water body. The method for detecting Cu < 2 + > has the advantages of simplicity in operation, high sensitivity, low detection limit and good selectivity, and has good quantitative detection capability on Cu < 2 + > in actual water, and the cadmium sulfide quantum dots are simple in synthesis, relatively low in cost and environment-friendly.