269 results about "Intense fluorescence" patented technology

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention discloses a light-emitting super-structure surface capable of realizing incoherent fluorescence holography and multi-dimensional super-structure display and a preparation method thereof. The light-emitting super-structure surface is mainly composed of an optical waveguide structure layer, a micro-nano structure layer and a fluorescence emission layer. The optical waveguide structure layer comprises a substrate and a waveguide layer and is responsible for guiding fluorescence propagation; the micro-nano structure layer is located above the waveguide layer, is composed of nano bricks and can regulate and control light; the fluorescence emission layer is arranged above the micro-nano structure layer, contains quantum dots and is excited by the pump light to emit fluorescence. The metasurface can regulate and control the emergent angle, wavefront and emergent intensity of fluorescent light and pump light, the time-space coherence of the fluorescent light is enhanced through the waveguide layer, and phase modulation is carried out by using a method based on a circuitous phase. Based on the super-structure surface, through the steps of fluorescence excitation, coherence enhancement, phase modulation, diffraction calculation, hologram generation, multi-dimensional display and the like, incoherent fluorescence hologram and multi-dimensional super-structure display can be achieved, and the functions of fluorescence nanometer printing and multi-channel holographic display are covered.

The invention relates to the technical field of image processing, in particular to endoscope equipment and an image fusion method. According to the endoscope equipment and the image fusion method provided by the invention, the current frame of white light image and the current frame of fluorescence image of the target detection part are collected, and after it is determined that the lens of the endoscope equipment moves, the current frame of fluorescence image is corrected according to the brightness change information of the current frame of white light image and the previous frame of white light image. Since the brightness change of the corresponding area of the white light image is positively correlated with the brightness change of the corresponding area of the fluorescent image, the fluorescent edge information of the fluorescent image is enhanced according to the brightness change information between the white light image frames, and the definition of the fluorescent edge of the target image after fusion processing is improved.

The utility model belongs to the technical field of fluorescence collection, and particularly relates to an efficient fluid particle fluorescence signal collection lens which comprises a box body, a fluorescence emitter used for providing fluorescence beams is arranged in the middle of the box body, and a reflection array mirror used for enhancing reflection of the fluorescence beams is arranged at one end in the box body. A refraction array focusing assembly used for refraction focusing of fluorescent light beams is arranged at the other end in the box body; a reflection array mirror is arranged at one end in a box body, and a refraction array focusing assembly is arranged at the other end in the box body, so that the reflection array mirror reflects a fluorescent light beam emitted by a fluorescent emitter through reflection, and the fluorescent light beam is enhanced through the reflection array mirror; and the focusing assembly is used for refracting and focusing the enhanced fluorescent light beam, so that the intensity of the fluorescent signal is increased, and the sensitivity, the signal collection efficiency and the signal-to-noise ratio of the fluorescent signal collection lens are improved.

The invention belongs to the field of low-dimensional semiconductor material micro-nano photoelectric integration, and particularly relates to a method for enhancing TMDs fluorescence based on a dark state energy transfer mechanism, a perovskite-TMDs heterojunction is obtained, then excitation is performed through a two-photon pump light source, dark state energy is accumulated, and space-prohibited dark state energy transfer is induced, so that the TMDs fluorescence is enhanced; the perovskite-TMDs heterojunction comprises a perovskite nanosheet and TMDs compounded on the surface of the perovskite nanosheet, and dark exciton state energy bands of perovskite and TMDs are matched. The invention also provides application of the method to improvement of fluorescence intensity in a device. The invention provides a brand new TMDs fluorescence enhancement thought, the TMDs and the perovskite are innovatively used for constructing a heterojunction system, and through combined control of heterojunction energy band matching components constructed by the TMDs and the perovskite and excitation of the two-photon pump light source, dark-state energy transfer can be induced, and the fluorescence enhancement effect of the two-photon pump light source is improved. Therefore, fluorescence enhancement of TMDs is realized based on a dark state energy transfer mechanism.

The invention discloses a fluorescence enhancement measurement system and method based on a silicon carbide silicon vacancy color center dielectric cavity, and the measurement system comprises a confocal system and a dielectric cavity sample, the confocal system comprises a laser, a reflector, a dichroscope, a filter, a scanning galvanometer, a microscope objective, a lens, an optical fiber connector, a single-mode optical fiber, a single-photon detector, a counting card and a computer. The confocal system is used for excitation of a dielectric cavity sample, efficient collection of fluorescence, micron-sized fluorescence space distribution imaging and reading and processing of a fluorescence signal; the dielectric cavity sample is used to generate and enhance fluorescence counts. The invention has the advantages of easiness in assembly and debugging, high sensitivity, wide application range, simplicity in processing, low cost and the like, can improve the fluorescence intensity of the silicon vacancy color center, and is used for high-sensitivity magnetic field measurement and improvement of the performance of a quantum sensing technology.

The invention belongs to a fluorescence analysis and detection method of p-nitrophenol, and particularly relates to a fluorescent carbon quantum dot probe for detecting p-nitrophenol as well as a preparation method and application of the fluorescent carbon quantum dot probe. The specific preparation method of the fluorescent carbon quantum dot probe comprises the following steps: taking aminopyrimidine as a precursor, mixing aminopyrimidine with water, and reacting at 160-200 DEG C to obtain the fluorescent carbon quantum dot probe with the surface rich in hydroxyl, amino and carboxyl groups. The fluorescent carbon quantum dot probe prepared by the invention has a strong fluorescent signal, selectively responds to p-nitrophenol, shows a wider linear detection range and high anti-interference capability, and is suitable for complex environmental pollutant detection.

The invention belongs to the field of functional nano materials and optical imaging, and particularly relates to a gold nanocluster and a preparation method and application thereof. The surface of the gold nanocluster contains a sulfydryl ligand A and a sulfydryl ligand B, the sulfydryl ligand A is ATT, and the sulfydryl ligand B is a sulfydryl ligand containing arginine; fluorescence quantum efficiency and electron transfer efficiency are synergistically enhanced on the basis of interaction of hydrogen bonds in the gold nanoclusters, the ultra-small luminous gold nanoclusters protected by double ligands are synthesized, the electrochemical luminescence quantum efficiency of the ultra-small luminous gold nanoclusters is up to 13.0% and is 4.2 times of that of a standard compound terpyridyl ruthenium in the field of electrochemical luminescence, and the ultra-small luminous gold nanoclusters are high in electrochemical luminescence quantum efficiency. An electrochemical luminescence transition area can be narrowed by 10 times compared with a terpyridyl ruthenium imaging system, and zero-thickness ECL luminescent layer cell high-resolution imaging can be realized; good application prospects are realized in the fields of fluorescence detection, electrochemical luminescence detection, fluorescence imaging, electrochemical luminescence imaging and the like.

The invention relates to the technical field of signal identification and positioning, and discloses a construction method and application of a fluorescence microscopic signal detection model. The invention relates to a fitting method based on self-supervised deep learning, which comprises the following steps of: representing a fluorescence microscope signal as a pattern of interest (POI) or a combination thereof, and executing parallel POI fitting and global denoising in a large receptive field by utilizing a self-supervised deep learning framework. The ultra-high-density fluorescence microscopic signal detection under the background of serious noise and strong fluorescence is realized in an unsupervised learning mode, and meanwhile, very high universality and usability are kept. The method can simply adapt to various fluorescence microscopic signal detection tasks by simply setting a POI example provided for the fluorescence microscopic signal detection model and adjusting a hyper-parameter related to sensitivity, and remarkable performance improvement is realized.

The present invention provides an antibody fluorescent labeling kit and a labeling method thereof, the kit comprising the following raw materials: 0.4-0.6mg fluorescein isothiocyanate, 500-700μL dimethyl sulfoxide, 2.0-3.5mL labeling buffer, 1.5-2.5mL labeling preservation solution, 0.1-0.3mL sodium bicarbonate solution, 1 centrifugal column, 3 EP tubes. The labeling method of the present invention completes the fluorescent labeling of the antibody by the following steps: S1, preparing antibody solution, S2, adjusting the pH of the antibody solution, S3, preparing fluorescein isothiocyanate solution, S4, preparing markers, S5, centrifugal column balancing treatment, S6, preparing fluorescently labeled antibodies, S7, fluorescently labeled antibody dilution and preservation. The kit of the present invention can quickly and conveniently label antibodies, and there is no need to go through a dialysis step during the entire labeling process, thereby simplifying the labeling process, saving labeling time, and being both convenient and efficient. The fluorescently labeled antibody prepared by the present invention has high fluorescence intensity and strong stability, and can still maintain strong fluorescence intensity even after being stored for 12 months.

The invention discloses a three-dimensional fluorescent nano printing method capable of being monitored in situ in real time, which comprises the following steps: (1) preparing a photoresist which comprises the following components: a two-step absorption photoinitiator, a small molecule polymerization monomer, aggregation-induced emission dye molecules and a photoresist auxiliary reagent; (2) a continuous laser beam acts on the photoresist through a three-dimensional scanning system, a double-step absorption photoinitiator generates an active factor through a double-step absorption process, a photopolymerization reaction of a small molecule polymerization monomer is initiated, and three-dimensional nano printing is achieved; in the photopolymerization region, the doped aggregation-induced emission dye molecules enable the printing structure to present strong fluorescence and the non-polymerization region to present weak fluorescence; and (3) rapidly scanning the three-dimensional nano printing area, obtaining the fluorescence intensity distribution of the three-dimensional nano printing structure in real time, and tracking and exploring the dynamic process and error correction of the three-dimensional nano printing. According to the invention, ultrahigh-precision fluorescent pattern three-dimensional nano printing and real-time in-situ detection can be realized.

The present application relates to an image processor and a computer-implemented image processing method for calculating a digital fluorescent output color image (160) in a medical fluorescent viewing device (100), the medical fluorescent viewing device has a white light color camera (110) for recording one or more digital white light color images (114) and a fluorescent color camera (111) for recording one or more digital fluorescent color images (112). The method and processor (170) are configured to retrieve a digital white light color image in which one or more fluorescent fluorophores (116, 118) are represented in a first imaged spectrum (202). The digital white color image comprises a plurality of first pixels (150a), and each first pixel (150a) comprises a first set ({R1, B1, G1}) of color space coordinates (R1, B1, G1). The first imaged spectrum (202) overlaps a fluorescence emission spectrum (226) of at least one fluorophore (116) of the one or more fluorescing fluorophores (116, 118). Further, a digital fluorescent color image (112) is retrieved in which one or more fluorescent fluorophores are represented in a second imaged spectrum (222) different from the first imaged spectrum. The digital fluorescent color image comprises a plurality of second pixels (150b), and each second pixel (150b) comprises a second set ({R2, B2, G2}) of color space coordinates (R2, G2, B2). The second imaged spectrum (222) overlaps a fluorescence emission spectrum of the at least one fluorophore. The digital fluorescent output color image is generated from a digital white light color image and a digital fluorescent color image, and includes a plurality of output pixels (150c). A color (R *, G *, B *) of an output pixel (150c) is calculated by applying a color conversion function (140) to an input union set (846, {R1, R2, G1, G2, B1, B2}) of a first set of color space coordinates of a first pixel (150a) and a second set of color space coordinates of a second pixel (150a). The use of union sets at each pixel allows for more reliable detection and enhancement of fluorescence-related image content, such as excitation fluorescence, autofluorescence, and / or reflections of objects illuminated with fluorescence excitation spectrums.

This invention provides a fluorescent labeling and conjugation method that exhibits enhanced signal intensity. [Solution] This disclosure relates to the field of fluorescent dyes, and more particularly to compositions and methods for increasing fluorescence signals and decreasing fluorescence quenching.

The utility model discloses a fluorescent strip for an aviation passenger cabin passage, which comprises a protective shell, a fluorescent strip and a connecting plate, the fluorescent strip is arranged in the protective shell, and the connecting plate is arranged at the bottom of the protective shell. According to the fluorescent strip for the aviation passenger cabin channel, the protective shell is hollow and matched with the fluorescent strip, the fluorescent strip can be protected against physical damage, the service life of the fluorescent strip is prolonged, the placement groove and the fluorescent strip are matched in size, a space is reserved, it is guaranteed that the fluorescent strip is stably installed and cannot be extruded to emit light, and the placement groove provides a stable installation position for the fluorescent strip; the dome design plays an important role, and the curved surface of the dome can diffuse light, so that fluorescence is uniformly distributed in the passenger cabin, and the visual range and definition are improved; meanwhile, the dome is resistant to pressure, can bear external treading force of people, disperse pressure, prevent the fluorescent strips from being damaged and maintain the emergency guiding function, and the reflectors on the two sides enhance fluorescent reflection, so that light rays are brighter and spread farther.

A fluorescence intensity enhancer containing a water-soluble compound having a melting point of 50° C. or higher, a fluorescence intensity enhancing method of a fluorescently labeled target biological substance using the fluorescence intensity enhancer, and a kit for fluorescence detection containing the fluorescence intensity enhancer.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The invention discloses a fluorescent material for rapidly detecting cesium ions in a water body, and a preparation method of the fluorescent material comprises the following steps: firstly, synthesizing a carbon quantum dot (NCD) with positive charges on the surface through a hydrothermal method, and then preparing an aqueous solution containing 0.1-100 mg.mL <-1 > of nitrogen-doped carbon quantum dots and 0.02-100 mg.mL <-1 > of heteropolyacid. The surface of the formed composite material presents negative charges, the original strong fluorescence effect is inhibited, and the composite material presents weak fluorescence, when a water body contains cesium ions capable of forming associates with heteropolyacid anions, the heteropolyacid anions are captured by the cesium ions, the strong fluorescence performance of the material is recovered, and it is indicated that the water body contains the cesium ions and is polluted. When the fluorescent material is used for detecting cesium ions, the sensitivity is high, the detection limit is low, rapidness is achieved, operation is easy, and the fluorescent material is especially suitable for monitoring seawater cesium ion pollution.

The invention discloses workshop cutting fluid aerosol detection equipment based on fluorescent carbon dots and a detection method, the workshop cutting fluid aerosol detection equipment comprises at least one pulse breathing machine, the pulse breathing machine is detachably connected with a filter screen, and the filter screen is used for capturing aerosol particles; the at least one ultraviolet light source is used for irradiating and exciting the aerosol particles; the optical lens is used for shooting the irradiated and excited filter screen to form a filter screen picture; and the image processing module is used for processing the filter screen picture and obtaining an RGB value. The structure of aerosol detection equipment can be simplified, the manufacturing cost can be reduced, the aerosol detection efficiency can be improved, meanwhile, use is flexible and convenient, the occupied area of a workshop is reduced, and the detection method is simple in step; aerosol formed after the cutting fluid is atomized is marked by utilizing the strong fluorescence property excited by the fluorescent carbon dots under an ultraviolet light source, so that the detection cost is greatly reduced, and the detection method is simple and efficient.

The invention discloses octavinyl polyhedral oligomeric silsesquioxane coupled brominated tetraphenylethylene as well as a preparation method and application thereof, and belongs to the field of fluorescent probes. Firstly, the rigid cage-shaped structure of POSS obviously enhances the aggregation-induced emission effect of TPE, so that the probe shows strong fluorescence emission in organic solvents such as DMF (Dimethyl Formamide) and the like; secondly, the probe shows an obvious solvent discoloration characteristic and shows adjustable optical response in different solvents, and meanwhile, the probe is endowed with excellent chemical stability by a POSS (Polyhedral Oligomeric Silsesquioxane) skeleton; thirdly, the probe has high selectivity and sensitivity recognition capability on heavy metal ions such as Fe < 2 + > and anions such as CN <->, the detection limit can reach 10 <-3 > mol / L, and visual detection can be realized through fluorescence change; in addition, a simple and convenient Heck coupling method is adopted for synthesis, the reaction condition is mild, the yield is up to 85%, and purification and large-scale production are easy.

The present application belongs to the technical field of food safety detection, and discloses a rapid Raman detection system and method for illegal additives in food; the illegal additives are enriched through dispersed liquid-liquid microextraction pretreatment, fluorescence emission spectrum data is obtained under low-power broadband excitation, and a fluorescence intensity gradient analysis is used to identify a fluorescence saturation risk section. The target spectrum range is divided into weak fluorescence and strong fluorescence collection windows arranged alternately, and differentiated collection parameters and processing strategies are used respectively; the offset excitation technology is used for the strong fluorescence window to obtain a difference spectrum; and the weak fluorescence window is used for accurate baseline deduction to generate a purified spectrum. Through splicing and connection, a complete composite purified spectrum is formed, and Raman true peaks and fluorescence false peaks are distinguished by combining peak identification and peak shape symmetry analysis. Finally, template matching is carried out based on peak position combination and intensity ratio characteristics, so that the illegal additives are accurately identified. The present application improves the accuracy, sensitivity and efficiency of food safety rapid screening.

The invention relates to a samarium-doped carbon quantum dot as well as a preparation method and application thereof, and belongs to the technical field of nano material synthesis, the preparation method comprises the following steps: carrying out ultrasonic dispersion on 0.01-0.03 g of p-phenylenediamine, 0.05-0.10 g of N-(4-aminophenyl) acetamide and 0.01-0.02 g of samarium chloride (III) hexahydrate in 15-25 mL of ultrapure water to form a mixed solution, carrying out hydrothermal reaction at 160-200 DEG C for 8-12 hours, filtering, washing and drying to obtain the samarium-doped carbon quantum dot. High-speed centrifugation, millipore filtration membrane filtration, 500Da molecular weight interception dialysis and freeze drying processes are sequentially adopted to realize efficient doping of the samarium element and precise purification of the product. The method is easy and convenient to operate, the product uniformity is excellent, and the prepared samarium-doped carbon quantum dots have the strong fluorescence characteristic and chemical stability and can be widely applied to the fields of biomedical imaging, photocatalytic materials and photo-thermal anticancer.

The invention belongs to the technical field of fluorescent probes, and relates to a preparation method and properties of two pH, viscosity and polarity three-response near-infrared fluorescent probes. The probe PODI and the probe PODB have the strongest fluorescence in an alkaline condition under excitation of 390nm, and the fluorescence is weakened when the acidity is enhanced; when excitation is carried out at 600nm, the fluorescence intensity is enhanced along with the increase of the glycerol content, and the viscosity change in acidic and neutral systems can be detected. The two probes present fluorescence response to polarity change: polarity-enhanced fluorescence rises under excitation of 390nm, and polarity-reduced fluorescence rises under excitation of 600nm. The preparation method is simple, high in yield and suitable for industrial production.

Described herein are methods and systems for training image to image translation machine-learning models, wherein the image to image translation machine-learning models are trained using unpaired images. The trained image to image translation machine-learning models can be used to digitally identify markers of a fluorescent image depicting a plurality of markers in a single color channel from a single fluorophore and / or enhance a fluorescence image.

A vibrationally enhanced fluorescence microscopy system includes a narrowband pulsed mid-infrared laser configured to excite selected chemical bond vibrations in a sample. A continuous-wave visible laser is coaligned with the mid-infrared laser and configured to induce fluorescence from at least one fluorescent reporter bound to the sample. An external pulse generator temporally synchronizes emission of the mid-infrared pulses with emission of the visible laser. A reflective objective is arranged to deliver the mid-infrared pulses to a focal plane within the sample. A water-immersion objective is coaxially aligned with the reflective objective and arranged to collect the fluorescence from the focal plane. A photon-counting detector is coupled to receive the collected fluorescence. Control electronics are configured to output a vibrational-contrast signal derived from counts produced by the photon-counting detector.

The invention discloses a method for preparing a fluorescent probe of plasma enhanced fluorescence and its application in ellagic acid detection, wherein anhydrous ethanol, TEOS and ammonia are reacted in a gold nanoparticle solution to obtain silicon dioxide gold nanoparticles, and reacted with APTMS to obtain amino-modified silicon dioxide-wrapped gold nanoparticles; sodium citrate, 3-APTES and glycerol are reacted to obtain a silicon dot solution, citric acid, EDC and NHS are added to form citric acid-modified silicon dots, and then reacted with EDC, NHS and amino-modified silicon dioxide-wrapped gold nanoparticle solution to obtain a silicon dot-modified gold nanoparticle solution on the surface of silicon dioxide; 3-aminophenylboronic acid, EDC and NHS are then added to react to obtain a fluorescent probe of plasma enhanced fluorescence. The fluorescent probe of plasma enhanced fluorescence prepared by the present invention has good stability, strong specificity and high sensitivity, and can be used for quantitative detection of ellagic acid and is more efficient, cost-saving and more accurate.

Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.

The invention discloses a preparation method of a recyclable orthogonal dynamic covalent fluorescent polymer material: connecting a phenylboronic acid group to lipoic acid or a lipoic acid derivative through an esterification reaction or an amidation reaction of its active ester to obtain benzyl lipoate borate and its derivatives or N-benzylboronic acid lipoamide and its derivatives. After heating and melting the above purified small molecules and removing water, transfer them to a substrate while it is still hot and spread them to obtain a yellow transparent supramolecular polymer. Irradiate it with an ultraviolet-visible light source to obtain the recyclable orthogonal dynamic covalent fluorescent polymer material containing disulfide bonds and borate ester bonds. The dynamic polymer material prepared by the invention is mainly crosslinked and self-assembled by dynamic disulfide bonds, dynamic borate ester bonds, and hydrogen bonds; it has strong fluorescence emission that can vary with temperature under light excitation at a wavelength of 400 nm; it has good biocompatibility, high stability, good homogeneity, and excellent mechanical properties. The preparation method of the invention is simple, and the process is safe and reliable.

The present invention discloses a method for preparing a metal corrosion inhibitor component, its products, and applications. The method involves reacting a halogenated tetraphenylethylene with aminopolyethylene glycol or aminoethanol to produce a metal corrosion inhibitor component. The component exhibits excellent corrosion inhibition performance and is easily detectable in corrosive media. Furthermore, the component emits strong fluorescence upon adsorption onto metal surfaces, enabling visual detection.

The invention relates to the technical field of fluorescence detection, in particular to an optical fiber fluorescence sensor and a fluorescence detection system.The optical fiber sensor is a conical optical fiber sensor, a gold nanoparticle layer is arranged on the conical optical fiber surface of the optical fiber sensor in a self-assembly mode, a spacer layer is arranged on the surface of the gold nanoparticle layer in a self-assembly mode, the spacing of the gold nanoparticles is 50-70 nm, and the thickness of the spacing layer is 5-15 nm. According to the tapered optical fiber fluorescence sensor based on LSPR enhanced fluorescence, an evanescent field is used for exciting LSPR resonance to generate a local strong electromagnetic field, so that the fluorescence is greatly enhanced, and the signal-to-noise ratio of the sensor is greatly improved. Through the structural design of the conical optical fiber and the adoption of a metal particle self-assembly technology, the problem of metal particle arrangement is solved, so that the particles are uniformly arranged, and the resonance field of the LSPR and the coupling of fluorescence and the optical fiber are balanced; and finally, a layer-by-layer self-assembly method is adopted, so that the polymer is used as a spacer layer between the metal particles and the fluorophores, and quenching of the fluorophores is prevented.

The invention relates to a breast cancer diagnosis probe, in particular to a fluorescence / magnetic resonance enhanced bimodal breast cancer imaging polypeptide probe as well as a preparation method and application thereof. The polypeptide probe is prepared from a fluorescence imaging unit, a magnetic resonance imaging unit, a self-assembly imaging unit and a TROP-2 targeted recognition unit. According to the invention, the polypeptide probe for enhancing fluorescence / magnetic resonance signals through in-situ self-assembly is constructed, and the polypeptide probe synthesized through modular optimization can realize in-situ self-assembly mediated by high-expression TROP-2 protein of triple negative breast cancer cells; self-assembly along with fluorescence and magnetic resonance unit aggregation can significantly enhance fluorescence and magnetic resonance bimodal imaging signals of triple-negative breast cancer tissues, improve imaging sensitivity and enhance triple-negative breast cancer imaging effects.