301 results about "Intense fluorescence" patented technology

A method for preparing carbon nitride quantum dots comprises the following steps of (1) material preparation, (2) drying and tabletting, (3) heating, (4) separation and (5) drying. Sodium chloride crystals are used as a template, the preparing process is simple and low in cost, and the sodium chloride crystals can be easily removed through water dissolution; melamine is used as the raw material, and annular carbon nitride basic structural units exist in melamine molecules, so that the yield of preparing the quantum dots is high, and product size uniformity is good; the prepared quantum dots are high in specific area, good in water solubility and good in dispersity, have strong fluorescent radiation visible under naked eyes, and have wide application prospects in the fields of fluorescence detection, light emitting devices, biomarker and the like.

The invention belongs to strong-fluorescence boron dipyrromethene dyes containing a carbazole structure in the technical field of organic chemical industry and fine chemical industry. Because a method for preparing the strong-fluorescence boron dipyrromethene dye containing the carbazole structure and derivatives thereof adopts boron dipyrromethene and carbazole aldehyde with substituent as raw materials, the molar ratio of the boron dipyrromethene to the carbazole aldehyde is 1:2-5, piperidine is a catalyst, a 4-angstrom molecular sieve is a dehydrating agent, and the molar ratio of the borondipyrromethene to the catalyst is 1:0.01-0.20; in an organic solvent, at the temperature of between 100 and 150 DEG C and under the protection of argon or nitrogen, the mixture reacts for 8 to 50 hours by stirring, the carbazole aldehyde and the active methylene of the boron dipyrromethene are dehydrated to generate the boron dipyrromethene derivatives; and after the carbazole structure with different substituents is introduced into the boron dipyrromethene, the optical property of the boron dipyrromethene compound is changed, the red shift of the absorption spectrum thereof is 80 nanometers, the red shift of the emission spectrum is 90 nanometers, higher fluorescence quantum yield is kept at 0.67, the laser efficiency reaches more than 30 percent, and the strong-fluorescence boron dipyrromethene derivatives containing the carbazole structure are obtained.

The invention provides a fluorescent probe represented by the formula (I), wherein R1 and R2 are independently selected from H or an aromatic vinyl group, and at least one of R1 and R2 is the aromatic vinyl group; R3 is selected from H, F, Cl, Br, OH, OCH3, N(CH3)2 or C1-C6 alkyl; and R4 is selected from C1-C6 alkyl. Compared with the prior art, because the fluorescent probe provided by the invention has a relatively large electronic conjugated system and plane, the intensity of the intramolecular charge transfer effect can affect the molecular fluorescence emission intensity; when the fluorescent probe and the G-quadruplex generate a specific action, the flexibility of intramolecular rotatable double bonds is restricted, the intramolecular charge transfer effect is enhanced, and the fluorescence is also enhanced significantly; moreover, the fluorescent probe provided by the invention has the advantages of low biotoxicity, phototoxicity and photobleaching property, good light stability, good water solubility and good cell membrane permeability.

The invention discloses a method for preparing graphene quantum dots through a carbon nano tube. The method comprises the following steps of: adding a mixed solution of nitric acid and sulfuric acid into the carbon nano tube, heating in a water bath, magnetically stirring, cooling to room temperature, diluting by using distilled water, regulating the pH value, performing dialysis and ultrafiltration centrifugation, and extracting through an organic solvent, so as to prepare the graphene quantum dots. The carbon nano tube which is diversified in growth modes and easy to control serves as a raw material, the graphene quantum dots are prepared through simple and controllable water bath heating and stirring operation; and the graphene quantum dot has the characteristics of good fluorescence effect, controllable fluorescence range, water solubility and the like, can be soluble with most of polymers through extraction and can be applied to the improvement of the device performance in photoelectric materials and devices.

The invention discloses a fluorescent probe used for detecting glutathione as well as a preparation method and application thereof. According to the invention, 1, 8-naphthalimide is used for building a classical ICT system, a phenyl sulfone part is introduced to the position of 4-, the ICT effect of a probe molecule is regulated and controlled, and due to the strong attracting electron effect of the phenyl sulfone in the position of the 4-, the probe molecule self is free from fluorescence emission. In case of the existence of glutathione, the phenyl sulfone part can be replaced by the glutathione, further the intramolecular electron transfer from sulphur atom in the position of the 4- (from glutathione) to the 1,8-naphthalimide can be generated, moreover, the ICT effect enables the probe molecule to send out strong fluorescence. The fluorescent probe provided by the invention can realize detection to the glutathione in cells, and has the advantages of being convenient and simple to operate, low in cost, sensitive in response, easy for popularization and application and the like.

The present invention relates to a two-photon fluorescent probe, more particularly a two-photon fluorescent probe represented by Formula 1, a method for preparing same and a method for imaging thiols in mitochondria using same. The two-photon fluorescent probe according to the present invention, having two probes introduced in one molecule, can selectively dye mitochondria and emit intense fluorescence by reacting with thiols. Accordingly, it can be used to image the distribution and activation of thiols in a living cell or an intact living tissue.

The invention discloses a fluorescent probe containing N, N-diethyl paramethylaminophenol and application thereof in the thiophenol detection. The structural formula of the fluorescent probe is as follows: in a phosphate buffered solution with pH value of 7.4, the compound can be in nucleophilic substitution reaction with thiophenol for releasing out the strong fluorescent material 3-(N, N-diethyl)-6-hydroxy-fluorane, so that the reaction system has a strong fluorescent signal at the wavelength of 543 nm; experimental results show that the fluorescent probe is used to detect the thiophenol; the detection limit of the thiophenol is 6.0*10<-9>mol/L; the relative standard deviation is 2.6%; the qualitative and quantitative detection of the thiophenol can be realized. The detecting method is simple in operation, high in accuracy and sensitivity and good in selectivity; different thiol compounds have no disturbance to the system of the fluorescent probe detecting the thiophenol; the fluorescent probe can be used for detecting the content of the thiophenol in a water sample.

The invention relates to an image fusion method and system. The method comprises the steps: obtaining a source image, wherein the source image comprises a fluorescence image and a visible light image;carrying out two-scale decomposition on the source image by adopting a Gaussian filtering method to obtain a base layer image and a detail layer image of the source image; constructing a weight map of the highlighted fluorescence information by using a nonlinear function; fusing the base layer image corresponding to the fluorescence image and the base layer image corresponding to the visible light image to obtain a base layer image of a fused image; constructing a second weight map for enhancing fluorescence information based on significance detection; fusing the detail layer image corresponding to the fluorescence image and the detail layer image corresponding to the visible light image to obtain a detail layer image of the fused image; and reconstructing the base layer image of the fused image and the detail layer image of the fused image to obtain a fused image. According to the invention, the complexity of a multi-scale algorithm can be reduced, and the image fusion efficiency isimproved.

The invention provides preparation and application of a mercury ion sensor based on photonic crystal enhanced fluorescence. The mercury ion sensor is composed of photonic crystal, a nucleic acid fluorescence molecular probe and a DNA assistant probe. The photonic crystal is used as a substrate, and strong affinity between thymidine (T) of DNA and Hg<2+> is employed to allow fluorescence group ROX labeled aptamer and a complementary sequence to compose a fluorescence detection system. A photonic crystal film is formed on a glass sheet substrate by prepared PS microspheres through vertical deposition self-assembly. Gold is sprayed on the surface of the photonic crystal; and based on Au-S bonding effect, mercapto group modified fluorescence group ROX labeled aptamer undergoes self-assembly on the surface of the photonic crystal, wherein the emission wavelength of ROX accords with the forbidden band of the photonic crystal, so fluorescence enhancing effect is achieved. According to the invention, fluorescence signal intensity of a nucleic acid fluorescence molecule is improved by using the principles of enhancement of fluorescence through the photonic crystal, so sensitivity and accuracy of sensor detection are improved.

The invention relates to a carbon quantum dot-copper ion fluorescent probe and a preparation method and application thereof. The method includes that carbon quantum dots with strong fluorescence are synthesized by the aid of a microwave hydrothermal method, fluorescence strength of the carbon quantum dots are greatly reduced by the aid of copper ions, the copper ions are composited on surfaces ofthe carbon quantum dots, surface energy is transferred, CQDs/Cu<2+> with weak fluorescence is formed, a glucose solution is added, pH value is adjusted to 7, heating is performed at the temperature of80DEG C, glucose and the copper ions undergo 'copper mirror reaction' to be oxidized to gluconic acid, and the copper ions are reduced to be precipitated to be Cu2O2. The fluorescence spectrum test shows that after the glucose solution is added, glucose is catalyzed and oxidized through the copper ions on the carbon quantum dots CQDs for reaction and is removed from surfaces of the CQDs, and thestrong fluorescence on the CQDs is restored. The carbon quantum dot-copper ion fluorescent probe based CQDs/Cu<2+> is applicable to effective detection of the glucose under non-enzymatic environment.

The invention provides a pyrene derivative fluorescent probe molecule for recognizing and detecting formaldehyde as well as a preparation method and application of the fluorescent probe molecule. Thepreparation method provided by the invention has simple synthetic steps only needs one step reaction and has a higher yield. The fluorescent probe provided by the invention does not have fluorescencein an HEPES buffer system, but can react specifically with the formaldehyde to generate a product with strong fluorescence, so as to achieve specific selection and quantitative analysis for the formaldehyde. The recognition characteristics of the probe B1 with aldehydes and amino acids in the HEPES buffer system are studied by a fluorescence spectrometer, results show that the probe B1 has high-efficiency specific selectivity for the formaldehyde, the lowest detection limit of the probe for the formaldehyde is 0.107[mu]M, and the probe has broad application prospects in the field of biomolecule detection.

The present invention discloses size controlled and stabilized nano-aggregates of molecular ultra small clusters of noble metals and a process for the preparation thereof. The present invention discloses single source multicolor noble metal spherical and uniform nano aggregates of 10-22 nm made up of discrete molecular ultra small noble metal (Nb) nanoclusters (MUSNbNC's) of 1-6 atoms. The MUSNbNC's are capped with amine/DCA (dicarboxy acetone) group acting as a steric and kinetic hindrance for core growth suppressing the further autocatalysis and conversion of super critical nucleus or growth by ripening and formation of nanoparticles and thus having intense fluorescence.

The invention belongs to the field of a nanometer material science and a laser Raman detection technology, and particularly relates to a preparation method of a self-assembled silver ball SERS (Surface Enhanced Raman Scattering) base with a controllable silver nano sheet thickness. The method takes silver nitrate as a silver source and L-ascorbic acid as a reducing agent, and comprises the following steps of: adding one organic acid with controlled amount to react in an ice bath (2-5 DEG C) for 2-15 minutes to obtain a silver micro-ball which is formed by self-assembling silver nano sheets with the controllable thickness in a range of 20-110nm. The silver micro-ball formed by self-assembling the silver nano sheets, which is prepared by the method, not only has the characteristic of high specific surface area of a common nano material, but also has the characteristics of controllable thickness of the surface silver nano sheets and serial and comparable surface roughness; and the silver micro-ball formed by self-assembling the silver nano sheets is a surface enhanced Raman base with the very good activity and is hopeful to be applied to aspects of surface enhanced fluorescence and photocatalysis.

The embodiment of the invention discloses an image fusion method for a medical fluorescence imaging system in the technical field of medical fluorescence imaging, and the method comprises the following steps: S1, carrying out the saliency region detection of a visible light image, obtaining a saliency map Svis, and carrying out the saliency region detection of a fluorescence image, and obtaining asaliency map Sflu; S2, calculating a fusion weight of the visible light saliency map and the infrared light saliency map; and S3, performing image fusion on the fluorescence image and the visible light image, and outputting a fusion image result. According to the method, the fusion weight is calculated by adopting the saliency map, and pseudo-color image fusion is carried out in the RGB color space, so that fluorescent region features can be highlighted, texture features of a fluorescent stimulated region can be enhanced, and the fused image is more natural and comfortable in impression; andthe fused image can effectively reserve key information in the image, enhance fluorescence characteristics in the fused image and highlight texture information of a fluorescence region.

The invention discloses a compound (I) P-N-methyl cyclopentaldehyde rhodamine 6G pH fluorescence molecular probe as well as a preparation method and use thereof. When hydrogen ions are present in the detected environment, under the action of hydrogen ions, the rhodamine 6G lactam spiral ring in the probe molecular compound (I) is opened in ring from closed state, and the color of the solution is obviously changed from achromatic color to orange red with strong fluorescence-emission. The compound I can be used for high-sensitivity naked eye color identification for hydrogen ions and also used as a fluorescent identifying probe.

The invention discloses a production method of silver-clad gold nano-rods and their application and belongs to the technical field of food quality detection and analysis. Au@ag nano-rods are produced, and with the surface enhanced fluorescence of the nano-rods, the nano-rods are successfully applied to the detection of the gene O157:H7easeA of E. coli. Compared with the traditional methods, the method has the advantages that detection limit is greatly lowered, sensitivity is improved, and good stability is achieved. According to the principle of fluorescence enhancement-weakening, a standard curve for gene O157:H7ease detection of E. coli is built, a linear range is from 10<-17> M to 10<-11> M, correlation coefficient R2 is 0.9947, and the detection limit is 3.33*10<-18> M (S/N = 3). The method has the advantages that specificity is good, labelling experiments show that eaeA gene recovery rate is 98.36% to 101.67%, with good stability, and the nano-rods are applicable to the detection of the gene O157:H7ease of E. coli and have a promising application prospect.

The invention relates to a method for quickly preparing a silver nanocluster with strong fluorescence emission. The method is characterized in that by volume, 14.75 parts of redistilled water is put into a container, 2.0 parts of AgNO3 solution, 0.25 parts of NaBH4 solution and 3.0 parts of amino black 10B are added into a thermostat water bath in sequence in a mixing condition, so as to be mixed for 3 to 90 min at 30-80 DEG C, and then the silver nanocluster with strong fluorescence emission can be obtained. According to the method, a biological dye of amino black is used as a stabilizing agent, and the silver nanocluster with strong fluorescence emission can be prepared after 10 min. The method has the advantages of easiness in obtaining raw materials, simple system, short synthetic time, strong fluorescence and smaller size.

The present invention provides pyrrole end-capped bipyridine derivative having a general formula (1) useful for the detection of zinc ions in solution. The present invention also provides a process for the preparation of pyrrole end-capped bipyridine derivative having a general formula (1). The present invention further provides a dipstick device by coating the assay powder of formula (1) in alumina over a thermoplastic or a glass solid support. The detection event can be monitored by noting the color change and the intense fluorescence change on the surface of the dipstick. For zinc ions, in addition to the color change an intense fluorescence change from greenish-yellow to red is observed. Detection event by means of fluorescence change is selective for Zn²⁺ ions when compared with all other biologically important metal ions like Na⁺, K⁺, Ca²⁺; Mg²⁺, Cu²⁺. The present dipstick is reusable, and can conduct the analysis of different samples using same stick.

The invention relates to a quinazolinone compound. The compound does not laminate in a solid state, and in the effects of hydrazine or amine substance gas, aminolysis or hydrolysis reaction of ester function group-O-C(O)-R3 is carried out, hydroxy is released, interaction between O-H of hydroxy and N of C=N in a quinazolinone ring is carried out, and an intramolecular six-membered ring hydrogen bond is formed; because of intramolecular rotation limit and excitation state intramolecular proton transfer mechanism, strong fluorescence is emit in a solid state. The compound can be used as a fluorescent probe for detecting hydrazine or amine substance gas.

The invention discloses a fluorescent microsphere co-doped with multiple near-infrared-II fluorescent dyes. The fluorescent microsphere comprises a macromolecule polymer microsphere and multiple near-infrared-II fluorescent dyes embedded in the macromolecule polymer microsphere. The invention also discloses a preparation method of the fluorescent microsphere. According to the invention, two or more near-infrared-II fluorescent dyes are embedded in a macromolecule polymer microsphere, and the ordered arrangement among molecules is reduced through different dye structures and mutual doping of molecular weights, so that the H aggregation and the J aggregation are further remarkably reduced, and the fluorescence intensity is enhanced so as to improve the detection sensitivity and the detectionaccuracy; and with the application of a fluorescent probe obtained by covalently coupling the fluorescent microsphere and a detection antibody in an immunodetection technology, the application prospect is wide.