155 results about "Fluorescent radiation" patented technology

Novel nanowell microarrays are disclosed in optical contact with polymer waveguides wherein evanescent field associated with lightwaves propagated in the waveguide excite target substances in the nanowells either by a common waveguide or by individual waveguides. Fluid samples are conveyed to the nanowells by means of microfluidics. The presence of the target substances in fluid samples is detected by sensing fluorescent radiation generated by fluorescent tag bound to the target substances. The fluorescent tags generate fluorescent radiation as a result of their excitation by the evanescent field. One or more PMT detectors or a CCD detector are located at the side of the waveguide opposite to the nanowells. Fluorescent radiation is detected due to its coupling with the waveguide or its emission through the waveguide.

A method for preparing carbon nitride quantum dots comprises the following steps of (1) material preparation, (2) drying and tabletting, (3) heating, (4) separation and (5) drying. Sodium chloride crystals are used as a template, the preparing process is simple and low in cost, and the sodium chloride crystals can be easily removed through water dissolution; melamine is used as the raw material, and annular carbon nitride basic structural units exist in melamine molecules, so that the yield of preparing the quantum dots is high, and product size uniformity is good; the prepared quantum dots are high in specific area, good in water solubility and good in dispersity, have strong fluorescent radiation visible under naked eyes, and have wide application prospects in the fields of fluorescence detection, light emitting devices, biomarker and the like.

An apparatus for selected measurement of in particular luminescent and/or fluorescent radiation has a plurality of measurement light paths (M1, M2, M3), which each have their own measuring devices optimized for the particular measurement mode. As a common device, a filter holder (5) is used, in which the detector and each of the measuring devices have access to each of the openings of the filter holder, and each of these openings can be equipped with an emissions filter that is expedient for the particular measurement mode involved. The result is an especially compact arrangement of a multipurpose measuring instrument with measurement properties that is largely equivalent to the measurement properties of special individual measuring instruments and that is also suitable in particular as an individual instrument for special luminescence measurements, such as BRET measurements.

An analysis of an object dyed with fluorescent coloring agents carried out with the aid of a fluorescent microscope which is modified for improved resolving power and called a nanoscope. The method is carried out with a microscope having an optical system for visualizing and projecting a sample image to a video camera which records and digitizes images of individual fluorescence molecules and nanoparticles at a low noise, a computer for recording and processing images, a sample holder arranged in front of an object lens, a fluorescent radiation exciting source and a set of replaceable suppression filters for separating the sample fluorescent light. Separately fluorescing visible molecules and nanoparticles are periodically formed in different object parts, the laser produces the oscillation thereof which is sufficient for recording the non-overlapping images of the molecules and nanoparticles and for decoloring already recorded fluorescent molecules, wherein tens of thousands of pictures of recorded individual molecule and nanoparticle images, in the form of stains having a diameter on the order of a fluorescent light wavelength multiplied by a microscope amplification, are processed by a computer for searching the coordinates of the stain centers and building the object image according to millions of calculated stain center co-ordinates corresponding to the co-ordinates of the individual fluorescent molecules and nanoparticles. With this invention it is possible to obtain a two-dimensional and a three-dimensional image with a resolving power better than 20 nm and to record a color image by dyeing proteins, nucleic acids and lipids with different coloring agents.

An apparatus for selected measurement of at least one of luminescent and fluorescent radiation from at least one sample well, the apparatus comprising: means defining an excitation light path for fluorescence measurements; at least one light source in the excitation path; means defining an emission light path; and at least one detector with a wavelength selector in the emission light path, wherein: the apparatus further comprises at least one first reflector element that encompasses a reflection chamber and projects at least a portion of the light emitted from the at least one sample well directionally onto the wavelength selector; the emission light path extends between the at least one sample well and the wavelength selector through the at least one first reflector element; and the excitation light path extends into the reflection chamber and extends to a point above the at least one sample well.

A laser cooling apparatus and method. Generally, the inventive apparatus includes a mechanism for transporting sensible thermal energy from a solid state laser and for communicating waste fluorescent radiation therefrom as well. In the illustrative embodiment, the apparatus includes an optically transparent manifold (10) with an inlet port (12), an exhaust port (19) and a plurality of spray nozzles (16) therebetween adapted to direct a cooling fluid on the laser medium (20) of a laser (30). In addition, the optically transparent manifold (10) is used to permit waste fluorescent radiation to escape the confines of the laser and cooling system means such that said fluorescent radiation may be optically directed to an external heat sink such as free space.

This invention discloses a laser diode pump all solid state ultraviolet pulse laser, the pump light output by coupling optic fiber, the pump light focused the pump end face of laser crystal through pump end face membrane to form plane reflection mirror, the laser crystal generating excited fluorescent radiation after absorbing pump light energy, the fluorescent forming base frequency light beam by oscillation in resonant cavity, laser incident to plane concave mirror by plane reflection mirror through laser crystal, sound and light Q adjusting crystal, plane color separation mirror, sum-frequency crystal and double frequency crystal, the plane color separation mirror leading light from resonant cavity, the focusing by lens, refracting by quartz prism and leading out laser by ultraviolet reflection mirror. Said invention has compact structure, high average output pulse power and large adjustable range of pulse recurrent frequency etc advantages.

For determining autofluorescence of a clinically normal skin issue (7) of a patient, the tissue (7) is irradiated with electromagnetic radiation. An amount of fluorescent radiation emitted by the tissue (7) in response to the irradiation is measured and, in response thereto, a signal is generated which represents a determined autofluorescence in agreement with the measured amount of electromagnetic radiation. Because the tissue (7) is skin tissue (7) in vivo and irradiation is performed noninvasively, a method which is very simple to apply for determining autofluorescence is obtained. An apparatus especially adapted for use in this method is also described.

A filter set for observing fluorescent radiation in biological tissue includes at least one illumination filter and at least one observation filter. The at least one illumination filter is arrangeable in an illumination system of an optical system. The at least one least one observation filter is arrangeable in an imaging system of the optical system.

A portable measurement apparatus is presented for inducing and measuring fluorescent X-ray radiation. It comprises an X-ray source (303, 902, 1005, 1105) adapted to controllably irradiate a sample (301, 803) with X-rays, and a detector (305, 406, 1006, 1106) adapted to detect fluorescent radiation emitted by said sample (301, 803). The X-ray source (303, 902, 1005, 1105) is an X-ray tube, an anode of which comprises at least one of silver, rhodium and molybdenium. Consequently said X-ray tube is adapted to controllably emit L-line radiation of at least one of silver, rhodium and molybdenium.

The invention provides a pulp-vitality fluorescence detection device and a detection method, by employing fluorescence for detection, the modulated exciting light after passing through a filter is transmitted by a transmitting fiber, and irradiates the surface of a detected tooth via a probe window and drives the tooth to produce fluorescent radiation, then the modulated exciting light is received and transmitted by a receiving fiber in the probe window, and is transferred to a glimmer detection unit via a receiving filter, and then the modulated exciting light enters into a data processing and control circuit for being controlled and processed. The invention resolves professional difficult problems which include painless detection of dental diseases and particularly the fast synthetic detection of pulp vitality, tooth decalcification and carious dentin, the detection is accurate, and the corresponding data can be directly displayed. By applying the invention, the detection is flexible because one point detection and multi-point detection can be chosen, the detection procedure is capable of rejecting operation mistakes, the detection is rapid, visual and accurate, the detection range is large, and the structure is simple. The invention can be used in places such as stomatological hospitals, dental clinics, etc., in which dental diseases are detected.

The invention provides a high efficiency coupling structure for extracting illumination such as fluorescent radiation from a chemical reaction vessel such as a cuvette. The cuvette is provided with a mirrored surface. An end cap for the cuvette includes a probe portion that exhibits total internal reflection. Lenses are provided in various embodiments that improve the light collection and directing properties of the end cap. A fast optical system for free space coupling of optical radiation emanating from a chemical processing cuvette that uses the end cap as an element is also described.

A glass or metal wire is precisely etched to form the paraboloidal or ellipsoidal shape of the final desired capillary optic. This shape is created by carefully controlling the withdrawal speed of the wire from an etchant bath. In the case of a complete ellipsoidal capillary, the etching operation is performed twice in opposite directions on adjacent wire segments. The etched wire undergoes a subsequent operation to create an extremely smooth surface. This surface is coated with a layer of material which is selected to maximize the reflectivity of the radiation. This reflective surface may be a single layer for wideband reflectivity, or a multilayer coating for optimizing the reflectivity in a narrower wavelength interval. The coated wire is built up with a reinforcing layer, typically by a plating operation. The initial wire is removed by either an etching procedure or mechanical force. Prior to removing the wire, the capillary is typically bonded to a support substrate. One option for attaching the wire to the substrate produces a monolithic structure by essentially burying it under a layer of plating which covers both the wire and the substrate. The capillary optic is used for efficiently collecting and redirecting the divergent radiation from a source which could be the anode of an x-ray tube, a plasma source, the fluorescent radiation from an electron microprobe, or some other source of radiation.

An X-ray source (101) is configured to controllably irradiate a sample (105) with incident X-rays through a sample window (104) that has a two-dimensional area. A detector (102) is configured to detect fluorescent radiation coming from the irradiated sample. A carrier (301, 401, 402, 501, 601) is essentially transparent to X-rays and disposed to spatially coincide with a substantial part of the two-dimensional area of the sample window (104). Marker material (303, 403, 602), which is responsive to X-rays by emitting fluorescent radiation, is mechanically supported by said carrier and essentially evenly distributed across at least that part of the carrier that spatially coincides with said two-dimensional area of the sample window.

A method of making shape-adaptable and spectral-selective distributed optical radiation sources, in the wavelength range between UV and mid-infrared, for therapeutic treatment using passive host medium containing nanocrystals is disclosed. The spectral output of the distributed optical radiation source is controlled by the nanocrystal size distribution that determines the spectral output of fluorescence radiation originating from these nanocrystals from within the said host medium, which contains the said nanocrystals, under excitation by an external source. The size of nanocrystals, or the size distribution of nanocrystals, incorporated in the host medium is selected based on the radiation spectral output required for therapeutic requirements. The passive host medium, incorporating the said nanocrystals, is made of adaptable, geometrically configurable, material that conforms to any desired shape.

A system for sorting a mixed stream of colors and types of cullet into separate groups of cullet with similar color and type includes a source for transmitting light through a cullet, and a camera having a plurality of pixels for receiving light transmitted through the cullet or detecting the absence of light blocked by an opaque contaminant, the camera providing at least one value from the light received, wherein the cullet moves along a designated path based in part on the at least one value. The system further includes a radiation source for irradiating a cullet with selected spectral ranges of radiation, and a sensor for determining characteristics of one or more selected spectral ranges of fluorescent radiation emitted by the cullet, wherein the cullet moves along a designated path based in part on the characteristics of the one or more selected spectral ranges of fluorescent radiation. The camera and the sensor collect the received light at desired sampling intervals and a circuit converts the output of the camera and the sensor into digital representation values. The circuit calculates a non-linear function from the digital representation values. An electrostatic or fluid driving actuator directs the cullet along a deflected path based on a value of the non-linear function. A vibratory feeder provides the cullet onto a conveyer belt having an exit roller of a desired diameter.

The invention discloses a preparation method of water-soluble CdSe quantum dots and belongs to the field of nanotechnology preparation. According to the method, sodium selenite is used as a selenium source, cadmium chloride is used as a cadmium source, mercaptopropionic acid and sodium hexametaphosphate are used as double complexing agents, and hydrazine hydrate deoxidizes the sodium selenite in an aqueous solution to prepare the water-soluble CdSe quantum dots in a one-step mode. The preparation method of the water-soluble CdSe quantum dots is simple and easy to operate, mild in conditions and suitable for batch preparation and large-scale synthesis. The prepared CdSe quantum dots are strong in fluorescent radiation in green light areas, and the radiation intensity can be controlled by controlling reaction time.

A method of monitoring a surface temperature of a hot gas path component includes directing an excitation beam having an excitation wavelength at a layer of a sensor material composition deposited on a hot gas path component to induce a fluorescent radiation. The method includes measuring fluorescent radiation emitted by the sensor material composition. The fluorescent radiation includes at least a first intensity at a first wavelength and a second intensity at a second wavelength. The surface temperature of the hot gas path component is determined based on a ratio of the first intensity at the first wavelength and the second intensity at the second wavelength of the fluorescent radiation emitted by the sensor material composition.