6 results about "Fluorescent tag" patented technology

This invention relates to the field of plant molecular breeding technology, specifically to a KASP molecular marker PH5-KASP based on the SNP site of the maize Zm00001eb259660 gene and its application. It provides a KASP molecular marker targeting the SNP site at 226029364 bp on maize chromosome 5, which is a functional missense mutation c.485C>T in the coding region of the Zm00001eb259660 gene. The marker consists of two allele-specific forward primers carrying different fluorescent tags and one universal reverse primer, stably distinguishing between C:C and T:T homozygous genotypes, where C:C corresponds to higher plant height and T:T corresponds to lower plant height. This invention offers high marker genotyping accuracy, simple and low-cost detection, and is suitable for large-scale breeding sample detection. It can be used for early identification of maize plant height traits, germplasm screening, and assisted breeding, significantly improving the efficiency of ideal maize plant architecture improvement.

The application relates to the field of agricultural biotechnology, and particularly discloses a KASP molecular marker for identifying the resistance of soybeans to phytophthora root rot and application. The KASP molecular marker primer sequence is shown in the upstream primer SEQ ID No. 1 (connected with a FAM fluorescent tag), the upstream primer SEQ ID No. 2 (connected with a VIC fluorescent tag) and the universal downstream primer SEQ ID No. 3. The KASP molecular marker can realize efficient, accurate and low-cost identification and genotyping of the resistance of soybeans to phytophthora root rot, and the effective identification and detection reaches 98.55%, and the accuracy reaches 84.80%.

This invention relates to the field of biotechnology and provides a gene-modified mouse model for tracking migratory organisms, its construction method, and its applications. The invention achieves precise labeling of endogenous migratory organisms by fusing the key structural protein TSPAN4 of the migratory organism with the fluorescent tag RFP and the modification tag Avi-tag, and site-specifically integrating it into the H11 site of mice, thus obtaining a TSPAN4-overexpressing transgenic mouse model. By introducing an LSL structure, the fusion gene is kept in a state of expression shutdown, achieving temporal and spatial specific activation only after mating with tissue-specific Cre tool mice. Multidimensional validation has shown that this model can accurately track migratory organisms in placental trophoblast cells, providing a standardized tool for assessing disease progression under physiological and pathological conditions through differences in migratory organism trajectory, inclusion packaging, and assembly, and can be directly applied to mechanistic studies and drug screening for diseases such as adverse pregnancy outcomes.

The application discloses a Ustilaginoidea virens dominant strain HEB07 and construction of a Ustilaginoidea virens pathogenic bacterium and millet interaction space-time atlas, relates to the technical field of biology, and the strain is screened and verified, the strain has the infection breadth of different millet varieties and the infection pathogenic sensitivity and survival rate of any millet variety, and has high affinity of recombination transformation of a GFP fluorescent label, so as to constitute a gene recombination strain using a green fluorescent protein GFP and explore the Ustilaginoidea virens pathogenic bacterium and millet interaction dominant strain. The application can provide important technical support for cloning research of millet disease-resistant genes, and provide strong scientific basis and technical means for cultivating millet varieties with high resistance to Ustilaginoidea virens.

Fluorophore-containing compounds for use as fluorescent tags for the detection or analysis of carbohydrates are provided. Also described are linkers for the preparation of the fluorescent compounds, methods of preparing and using the fluorescent compounds, and carbohydrate conjugates formed from the compounds.

Disclosed probes comprise metal nanoparticle cores associated with magnetic particles that allow probes associated with targets to be concentrated by an applied magnetic field to increase detection sensitivity and provide sufficient spacing between concentrated probes to avoid signal quenching. The probe may comprise at least one recognition receptor, and may further comprise at least one reporter molecule, such as a fluorescent tag, a Raman reporter, or combinations thereof. Concentrating probe-target composites substantially enhances a sensing signal, such as from 5 to 10 times, compared to detection without concentrating the probes. The method may be used to detect, for example, interleukins at concentrations at least as low as 25 pg / ml in sputum or blood from a subject for early and precise profiling of viral infections, such as SARS-CoV-2 infections.