14 results about "Fluorescent tag" patented technology

This invention relates to the field of plant molecular breeding technology, specifically to a KASP molecular marker PH5-KASP based on the SNP site of the maize Zm00001eb259660 gene and its application. It provides a KASP molecular marker targeting the SNP site at 226029364 bp on maize chromosome 5, which is a functional missense mutation c.485C>T in the coding region of the Zm00001eb259660 gene. The marker consists of two allele-specific forward primers carrying different fluorescent tags and one universal reverse primer, stably distinguishing between C:C and T:T homozygous genotypes, where C:C corresponds to higher plant height and T:T corresponds to lower plant height. This invention offers high marker genotyping accuracy, simple and low-cost detection, and is suitable for large-scale breeding sample detection. It can be used for early identification of maize plant height traits, germplasm screening, and assisted breeding, significantly improving the efficiency of ideal maize plant architecture improvement.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

This invention provides a KASP molecular marker associated with wheat resistance to mesosulfuron-methyl and its application, belonging to the field of crop molecular genetics and breeding technology. Association analysis revealed a significant association between this KASP molecular marker and the mesosulfuron-methyl resistance trait, exhibiting T / C polymorphism. Specifically, CC homozygous wheat showed significantly higher resistance to mesosulfuron-methyl than TT homozygous wheat. Based on this, a KASP marker reagent or kit containing a specific primer set was developed. The primer set includes two allele-specific upstream primers and one universal downstream primer, with FAM and HEX fluorescent tags attached to the 5' ends of the upstream primers, respectively. High-throughput genotyping can be achieved by optimizing the PCR reaction system and landing procedure. The application of this KASP molecular marker can rapidly screen wheat varieties resistant to mesosulfuron-methyl, providing technical support for herbicide-resistant molecular breeding, significantly improving the efficiency of resistant variety selection, and playing a vital role in ensuring safe wheat production.

The application relates to the field of agricultural biotechnology, and particularly discloses a KASP molecular marker for identifying the resistance of soybeans to phytophthora root rot and application. The KASP molecular marker primer sequence is shown in the upstream primer SEQ ID No. 1 (connected with a FAM fluorescent tag), the upstream primer SEQ ID No. 2 (connected with a VIC fluorescent tag) and the universal downstream primer SEQ ID No. 3. The KASP molecular marker can realize efficient, accurate and low-cost identification and genotyping of the resistance of soybeans to phytophthora root rot, and the effective identification and detection reaches 98.55%, and the accuracy reaches 84.80%.

The present application relates to a method for detecting and / or screening a target protein expression cell line based on fluorescence indication, aiming to realize rapid detection of specific protein conformation in a chassis cell, and at the same time, to efficiently screen chassis cells with different functional states, activities and target protein expression levels. The essence of the present technology is different from the conventional protein fusion fluorescent tag used to indicate the protein. The present method first reflects the conformational change of the protein through a cross-linking agent, and reflects the functional state corresponding to the conformation of the protein based on the change of the fluorescence color of the cross-linking agent skeleton under different protein conformations. This technology provides a convenient, efficient and non-invasive method for the field of biological research, which can be widely used in the fields of cell activity research, drug screening and protein function research.

The invention provides a recombinant coccidiosis vector for expressing VP2 protein and a fluorescent label and a detection method of the recombinant coccidiosis vector, and belongs to the technical field of genetic engineering. The invention also provides sgRNA (small guide ribonucleic acid) targeting the ETH00009555 gene, and the sgRNA can be used for carrying out accurate gene fixed-point insertion; a gene editing system is established by sgRNA and homologous recombinant fragments, so that target fragments such as chicken infectious bursal disease virus VP2 protein and fluorescent label protein can be accurately subjected to homologous recombination on the ETH00009555 gene, and a recombinant coccidiosis vector for expressing the VP2 protein and a fluorescent label is constructed. The recombinant coccidium vector DNA is subjected to PCR (Polymerase Chain Reaction) detection and sequencing, and the result shows that the VP2 gene and the fluorescent tag gene are successfully recombined with the ETH00009555 gene; and the recombinant coccidiosis vector has an obvious fluorescence signal under a fluorescence microscope, which indicates that the recombinant coccidiosis vector successfully expresses the VP2 protein and the fluorescence tag protein.

A microfluidic reaction chamber with a reaction chamber circuit includes a microfluidic reaction chamber to contain a reaction fluid for amplification of nucleic acids, and a reaction chamber circuit disposed within the microfluidic reaction chamber. The microfluidic reaction chamber includes a base wall, a top wall parallel to the base wall and defined in part by a transparent lid, a first side wall, and a second side wall. The reaction chamber circuit is disposed within the microfluidic reaction chamber, and includes a top surface, a bottom surface, a first side wall, and a second side wall. The reaction chamber circuit is in fluidic contact with the reaction fluid and includes a photodetector to detect a fluorescence signal from a labeled fluorescent tag in the reaction fluid.

The present invention addresses the problem of providing: a nerve cell analysis method with which the localized amount of TDP-43 in nerve cells can be analyzed without labeling TDP-43 with a fluorescent tag or the like and without carrying out gene transfer; a kit for carrying out the nerve cell analysis method; and a screening method for a prophylactic and / or therapeutic agent for a neurodegenerative disease. The present invention provides a nerve cell analysis method involving: a staining step for immunofluorescent staining of nerve cells using an anti-TDP-43 antibody and an antibody that recognizes a stress granule marker; a cell region identification step for identifying the cytoplasm and nuclei of the nerve cells; and an analysis step for analyzing a TDP-43-derived fluorescence signal and a stress granule marker-derived fluorescence signal in the cytoplasm, and analyzing the localized amount of TDP-43 in the nerve cells on the basis of the presence or absence of colocalization of a granular TDP-43 signal and a granular stress granule marker signal in the cytoplasm.

This invention relates to the field of biotechnology and provides a gene-modified mouse model for tracking migratory organisms, its construction method, and its applications. The invention achieves precise labeling of endogenous migratory organisms by fusing the key structural protein TSPAN4 of the migratory organism with the fluorescent tag RFP and the modification tag Avi-tag, and site-specifically integrating it into the H11 site of mice, thus obtaining a TSPAN4-overexpressing transgenic mouse model. By introducing an LSL structure, the fusion gene is kept in a state of expression shutdown, achieving temporal and spatial specific activation only after mating with tissue-specific Cre tool mice. Multidimensional validation has shown that this model can accurately track migratory organisms in placental trophoblast cells, providing a standardized tool for assessing disease progression under physiological and pathological conditions through differences in migratory organism trajectory, inclusion packaging, and assembly, and can be directly applied to mechanistic studies and drug screening for diseases such as adverse pregnancy outcomes.

The application discloses a Ustilaginoidea virens dominant strain HEB07 and construction of a Ustilaginoidea virens pathogenic bacterium and millet interaction space-time atlas, relates to the technical field of biology, and the strain is screened and verified, the strain has the infection breadth of different millet varieties and the infection pathogenic sensitivity and survival rate of any millet variety, and has high affinity of recombination transformation of a GFP fluorescent label, so as to constitute a gene recombination strain using a green fluorescent protein GFP and explore the Ustilaginoidea virens pathogenic bacterium and millet interaction dominant strain. The application can provide important technical support for cloning research of millet disease-resistant genes, and provide strong scientific basis and technical means for cultivating millet varieties with high resistance to Ustilaginoidea virens.

The application discloses a recombinant protein for detecting paraneoplastic Yo antibody by CBA method and application, and belongs to the technical field of biological medicine and engineering. The amino acid sequence of the recombinant protein comprises, in sequence, a secretion signal peptide, a CDR2 protein partial sequence, a CDR2L protein partial sequence, a transmembrane region and a fluorescent label. The secretion signal peptide is a human albumin signal peptide ALB. The transmembrane region is CD8a hinge. The fluorescent label is mCherry. The application constructs a novel recombinant protein which can be stably overexpressed on the cell membrane of eukaryotic cells by intercepting specific partial sequences of CDR2 protein and CDR2L protein, and redesigning and fusing a fluorescent label by using a secretion signal peptide, a transmembrane sequence and a connecting peptide. The recombinant protein retains the core antigen regions of the two, can effectively overcome the insufficiency in sensitivity and specificity, can significantly improve the detection rate of paraneoplastic Yo antibody when CBA method is used for detection, and can reduce the false positive rate, so that the requirement of clinical detection can be better met.

Fluorophore-containing compounds for use as fluorescent tags for the detection or analysis of carbohydrates are provided. Also described are linkers for the preparation of the fluorescent compounds, methods of preparing and using the fluorescent compounds, and carbohydrate conjugates formed from the compounds.

The application discloses a CBA method reagent for detecting GFAP antibody by fusing expression of GFAP alpha and GFAP epsilon and a detection method, and belongs to the technical field of neuroimmunological diagnosis. The reagent comprises a host cell which is recombined to express a fusion protein. The fusion protein comprises the following functional domains in sequence: a secretion signal peptide, a GFAP alpha partial sequence, a GFAP epsilon partial sequence, a transmembrane sequence and a fluorescent label. The core of the application is that the fusion cell is constructed by using the partial sequences of GFAP alpha and GFAP epsilon, and the co-expression is realized in the host cell by using the co-transfection technology. The sensitivity and the specificity of the detection of the CFAP antibody in the CBA method (cellular immunofluorescence method) can be significantly improved.

Disclosed probes comprise metal nanoparticle cores associated with magnetic particles that allow probes associated with targets to be concentrated by an applied magnetic field to increase detection sensitivity and provide sufficient spacing between concentrated probes to avoid signal quenching. The probe may comprise at least one recognition receptor, and may further comprise at least one reporter molecule, such as a fluorescent tag, a Raman reporter, or combinations thereof. Concentrating probe-target composites substantially enhances a sensing signal, such as from 5 to 10 times, compared to detection without concentrating the probes. The method may be used to detect, for example, interleukins at concentrations at least as low as 25 pg / ml in sputum or blood from a subject for early and precise profiling of viral infections, such as SARS-CoV-2 infections.