37 results about "Water Channel Proteins" patented technology

Personal care composition comprising from about 0.05% to about 5% of at least one aquaporin-stimulating compound selected from the group consisting of xanthine, caffeine; 2-amino-6-methyl-mercaptopurine; 1-methyl xanthine; 2-aminopurine; theophylline; theobromine; adenine; adenosine; kinetin; p-chlorophenoxyacetic acid; 2,4-dichlorophenoxyacetic acid; indole-3-butyric acid; indole-3-acetic acid methyl ester; beta-naphthoxyacetic acid; 2,3,5-triiodobenzoic acid; adenine hemisulfate; n-benzyl-9-(2-tetrahydropyranyl)adenine; 1,3-diphenylurea; 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea; zeatin; indole-3-acetic acid; 6-benzylaminopurine; alpha-napthaleneacetic acid; 6-2-furoylaminopurine; green tea extract; white tea extract; menthol; tea tree oil; ginsenoside-RB1; ginsenoside-RB3; ginsenoside-RC; ginsenoside-RD; ginsenoside-RE; ginsenoside-RG1; ginseng root extract; ginseng flower extract; pomegranate extract, extracts from Ajuga turkestanica; extracts from viola tricolor and combinations thereof; an additional ingredient selected from the group consisting of niacinamide, glycerin and mixtures thereof, and a dermatologically-acceptable carrier.

A water purifying filter and method for fabricating the same are disclosed. The water purifying filter includes a support substrate, a pattern formed on the support substrate to have at least one hole, membrane protein of an aquaporin group coated on the pattern and a protective layer formed on polymer pattern having the membrane protein coated thereon.

The present invention relates to a method for detecting antibodies against a target antigen in a sample which comprises contacting the sample with labelled target antigen, subjecting the sample to immunoprecipitation to precipitate antibodies in the sample and detecting the presence of antibodies against the target antigen in the sample by means of the presence of labelled target antigen in the immunoprecipitate, wherein the labelled target antigen is a fusion protein comprising the target antigen and a fluorescent protein label and the presence of labelled target antigen in the immunoprecipitate is detected by means of the fluorescence of the fluorescent label. The method is particularly suitable for use where the target antigen is an autoantigen and can also be used to identify autoantigens implicated in a particular autoimmune disorder by screening serum samples from patients with a clinical phenotype indicative or suggestive of an autoimmune disorder and suitable controls. The target protein may be from the cys-loop acetyl choline receptor ion channel gene superfamily, the voltage-gated calcium, sodium or potassium ion channel gene superfamily, the glutamate receptor gene family, a receptor tyrosine kinase, or other membrane associated channels such as aquaporin gene family.

The invention discloses a moisturizing and repairing composition for stimulating cellular water channel protein production. The active ingredients of the moisturizing and repairing composition are composed of hydrolyzed viola tricolor extract, sodium hyaluronate, bio-saccharide gum-1, ceramide 3 and tripeptide-1 copper. The invention further provides application of the moisturizing and repairing composition in preparation of cosmetics with moisturizing and repairing effects and cosmetics comprising the moisturizing and repairing composition. According to synergistic effects of the various active ingredients, the moisturizing and repairing composition effectively stimulates cellular water channel protein production and achieves excellent effects of moisturizing and repairing skin barrier.

The invention discloses a vesicle with amine reaction functional groups. The vesicle comprises ABA or AB amphiphilic block copolymers and water channel proteins. The water channel proteins are embedded in the ABA or AB amphiphilic block copolymers, and the amine reaction functional groups capable of participating in polyamide interfacial polymerization reaction are grafted on the ABA or AB amphiphilic block copolymers. The invention further provides application of the vesicle with the amine reaction functional groups to preparing a reverse osmosis membrane. The vesicle and the application havethe advantages that amine graft modification is carried out on the vesicle with the water channel proteins, accordingly, the vesicle with the water channel proteins can participate in the polyamide interfacial polymerization reaction, not only can be packaged in hyper-cross-linked structures inside polyamide, but also can be connected with the hyper-cross-linked structures inside the polyamide bychemical bonds and is stable inside the reverse osmosis membrane, the problems of translocation, deformation, rupture and the like of existing vesicles with existing water channel proteins in osmosisprocedures can be solved, and the service life of the reverse osmosis membrane with the water channel proteins can be greatly prolonged.

The invention discloses a gene AtPIP2;7 capable of improving plant disease resistance and application thereof. The invention clones an arabidopsis thaliana water channel protein AtPIP2;7 gene from cruciferous plant arabidopsis thaliana, and based on the gene, constructs an AtPIP2;7 overexpression vector which can be used in the arabidopsis thaliana, tobacco, rice and other plants. After the gene AtPIP2;7, or protein, a recombinant expression vector or a transformant encoded thereby is introduced into the arabidopsis thaliana, a variety having significant disease resistance and/or capable of promoting plant growth can be obtained. The gene AtPIP2;7 can be applied to improvement of disease resistance in crop breeding, and is expected to improve the disease resistance of plants, thereby achieving the purposes of increasing yield and reducing pesticide.

The invention relates to a rice aquaporin gene OsPIP2;7 plant expression vector and application thereof. The plant expression vector is pHB-OsPIP2;7-3*HA. The nucleotide sequence of the plant expression vector is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The terminator sequence of a gene OsPIP2;7 is replaced with a base sequence of a 3*HA tag, the modified OsPIP2;7-3*HA gene sequence is inserted into a cloning vector pMD19-T simple vector and is bonded to Hind III and Xba I loci of a binary vector pHB after double enzyme digestion of Hind III and Xba I, and the plant expression vector is obtained. After the plant expression vector is used for transforming plants, a target protein OsPIP2;7 is greatly expressed in the plants, introduction of the 3*HA tag solves the problem that in the experiment process that an aquaporin antibody is used for detecting the expression quantity of the target protein, a large quantity of homologous proteins are detected and accordingly the detection quantity of OsPIP2;7 is not accurately, and experiment bases are provided for research of new functions of OsPIP2;7.

The invention discloses an application of a water channel protein coded gene OsNIP1;1 of rice. The registered number of the water channel protein coded gene OsNIP1;1 in Genbank is AB856419. The gene can be applied to reducing the concentration of trivalent As of rice stele parts, reducing loading of the trivalent As to xylem and notably reducing arsenic accumulation of overground parts and grainsof the rice. Through massive experiment, the inventor finds the biological function of the water channel protein coded gene OsNIP1;1 in the rice, and after the gene is subjected to overexpression, theAs content of the overground parts of the rice is reduced. After the gene is subjected to overexpression, the accumulation of As in leaves, seed shells and grains of the rice can be notably reduced.

The invention relates to a terahertz wave technology-based unmarked aquaporin function assessment method. According to the method, cells are inoculated on the surface of a constructed terahertz metamaterial chip, culture is carried out to ensure that the cells grow in an adherent state and are fused into a single-cell layer, and a terahertz time-domain spectrograph is adopted to measure the harmonic peak condition of a cell layer in isotonic buffer solution; and hypotonic buffer solution is changed, and a large number of water molecules enter the cells through aquaporins to ensure that the volumes of the initial cells are increased so as to form swelled cells. According to the method, the terahertz time-domain spectrograph is adopted to obtain the harmonic peak change condition of the adherent cell layer during osmotic pressure change, so that the cell swelling degree is effectively reflected, namely, the osmosis condition of the water molecules passing through the cell layer; and thereciprocal of a time required for achieving a maximum response value is solved according to a real-time response curve, so that the unmarked assessment of the cell aquaporin function condition can berealized. The method can be used for high-throughput screening of correlated targeted AQP drugs.

The invention discloses a screening method of a banana water channel protein gene promoter core area. The screening method comprises the steps as follows: control selection: transgenic arabidopsis forconverting a CaMV35S promoter as a control; detection of promoter GUS activity: promoter GUS activity detection is performed on leaves and root systems of 15-day transgenic Arabidopsis seedlings treated by 100 mM, 200 mM and 300 mM 18% PEG 6000; and observation of staining condition: seen from phenotype, in GUS staining, except CaMV35S control plants, promoter transgenic plant leaves of each fragment is not stained or lightly stained while root tip parts are deeply stained in transgenic root systems. According to the result, the core area of MaPIP1:1 promoter is recognized, and the acting mechanism of the MaPIP1;1 gene during resisting of drought stress can be further illuminated.

The invention discloses a method to prepare proteoliposomes using glycerol or polyethylene glycols (PEG) in the rehydration step. The method eliminates the use of expensive surfactants and subsequent time-consuming removal of those surfactants during the preparation of proteoliposomes. The fusible proteoliposome reconstituted with phage portal proteins or other hydrophobic channel proteins are useful for nanopore sensing technology, including ultrafast DNA sequencing and biomedical diagnostic applications.

The present invention relates to self-assembled nano structures comprising polyalkyleneimine (PAI) and a detergent solubilized transmembrane protein, such as an aquaporin protein.

The invention relates to a post-weaning piglet nutritional diarrhea model, specifically relates to the high-casein ration-induced type post-weaning piglet nutritional diarrhea model, and belongs to the field of establishment of animal models. A establishing method of the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is characterized in that a 21-day post-weaning piglet is continuously fed for a high-casein ration for 15 days, casein in the high-casein ration is a unique source of protein, the adding amount of the casein is 33.72%, and the content of crude protein in the ration is 30%; and after a test is finished, a piglet diarrhea index, a serum biochemical index, an intestinal structure form and the expression amounts of an intestinal water channel protein marker molecule and a tight junction protein marker molecule in the piglet are measured. The establishing method involved in the invention has the beneficial effects that the casein is used as the unique source of the protein, the adding amount of the casein in the ration is determined, and the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is established, so thata reliable model is provided for the deep exploration and prevention of post-weaning diarrhea of newborn animals.

Methods of fabricating a membrane comprising proteoliposomes having protein water channels are provided herein. The method may include providing a porous substrate, depositing a solution containing proteoliposomes on the porous substrate, and then contacting the porous substrate with an aqueous monomer solution and an organic monomer solution to form a selective layer on the porous substrate embedding the proteoliposomes. The method may include depositing the aqueous monomer solution, then the solution containing the proteoliposomes, then the organic monomer solution, to form the selective layer. The present disclosure also describes the membrane and a system operable to accommodate both methods.

The present invention discloses a method for cloning reference gene of kentucky bluegrass aquaporin. The method comprises: (1) adopting the total RNA of the kentucky bluegrass as a template to carry out a reverse transcription reaction to obtain a first strand of the cDNA; (2) adopting the cDNA as a template, and adopting EF1alphaF1 as the primer to carry out PCR amplification; (3) carrying out recovery for the amplification product to recover the target gene band; (4) ligating the resulting target gene to a pMD18-T vector, and transforming the resulting vector into competent cells, then carrying out DNA sequencing for the cultured bacteria liquid to obtain the reference gene sequence of the kentucky bluegrass aquaporin, wherein the reference gene sequence comprises 176 bp. Compared to the traditional reference gene, the obtained reference gene EF-1alpha of the kentucky bluegrass is prevented from the influence due to the cell growth state, can undergo the influence due to external non-biological factors, and can be expressed stably in the tissue.

The invention discloses a screening method of a banana aquaporin gene promoter transcription factor. The screening method includes the following steps: a core region of a promoter is judged, wherein according to a banana A genome, primers of the promoter are designed and amplified, the amplification length is 1362 bp, through plantcare and PLACE, cis-acting elements of the promoter are analyzed, GUS activity of the promoter is detected, and through dyeing conditions, the core region B of the promoter is judged; and yeast one-hybrid is conducted to screen the transcription factor interacting with the promoter, wherein the yeast one-hybrid method is used for verification, the core region B of the promoter serves as a bait section for building a bait carrier, and a banana one-hybrid library obtained through yeast drought treatment is screened. By adopting the screening method of the banana aquaporin gene promoter transcription factor, the transcription factor which is directly bonded to the MaPIP1;1 promoter under drought stress conditions is identified, therefore, research for banana aquaporin genes is more convenient, the draught resistance capability of transgenic bananas can be improved, and a draught resistance mechanism of MaPIP1;1 can be further understood.

The invention provides a water-channel protein gene relative with sprouting of microsporidia of silkworms. The gene sequence CDS for coding the water-channel protein NbAQP for microsporidia of silkworms is retrieved based on the 9-magification gene data of microsporidia of silkworm, and is proved by a series of biologic cloning technologies. The water-channel protein NbAQP got by the invention is of high importance for studying the sprouting mechanism of microsporidia, hence can play role in preventing and treating microsporidia in production practice. According RNAi interference, after the water-channel protein NbAQP being interfered, the microsporidia loses the capability for infecting the host, this provides a forceful means for preventing and treating granulosis of silkworms; in addition, by means of transgenosis, the expression of the water-channel protein gene can be enriched, so that the water-channel protein gene can be used to control such destructive insects as locusts, etc.

The invention discloses an application of a water channel protein coded gene OsNIP3;3 of rice. The registered number of the water channel protein coded gene OsNIP3;3 in Genbank is AB710141. The gene can be applied to reducing the concentration of trivalent As of rice stele parts, reducing loading of the trivalent As to xylem and notably reducing arsenic accumulation of overground parts and grainsof the rice. Through massive experiment, the inventor finds the biological function of the water channel protein coded gene OsNIP3;3 in the rice, and after the gene is subjected to overexpression, theAs content of the overground parts of the rice is reduced. After the gene is subjected to overexpression, the accumulation of As in leaves, seed shells and grains of the rice can be notably reduced.

The invention provides a low-deuterium water ecological breast enlargement instrument, which comprises a semiconductor refrigerating sheet, a cold water tank, a hot water tank, a water pump, an air pump, a three-way valve, a radiator, a bra and a controller. Between that cold water tank and the hot water tank, a semiconductor refrigerate sheet is arranged; the water pump is installed at the outletpipe, the 3-way valve is arrange at that water pipe interchange, A radiator is arranged on the circulating water pipe, and the air pump is connected with the bra through the trachea. As the low deuterium water replaces the common purify water and flows between the hot water tank, the cold water tank and the bra through the water pipe, the low deuterium water permeability is stronger than that ofthe common purified water, and the low deuterium water small molecular cluster can provide a low deuterium environment for the cell during the heat energy conversion, and the ordered and structured low deuterium water small molecular cluster can repair and activate the water channel proteins.