Fabrication of aquaporin-based biomimetic membrane

A water channel and protein technology, applied in membrane, membrane technology, semi-permeable membrane separation, etc., can solve the problems of easy damage and unprotected water channel protein.

Pending Publication Date: 2021-10-22
NANYANG TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aquaporins may also be exposed and not properly protected in the resulting membrane, which makes aquapori...

Method used

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  • Fabrication of aquaporin-based biomimetic membrane
  • Fabrication of aquaporin-based biomimetic membrane
  • Fabrication of aquaporin-based biomimetic membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0090] Example 1A: System Overview

[0091] The present disclosure describes a method of producing interfacially polymerized ABMs in a cost-effective manner compared to conventional methods. A scheme describing this method is shown in figure 1 .

[0092] The proteoliposome solution is sprayed on the surface of the membrane substrate before soaking the membrane substrate or the surface of the membrane substrate in the aqueous monomer solution. After drying with nitrogen, the surface was exposed to an aqueous monomer solution for a certain period of time, followed by an organic phase polymerization procedure. Compared with membranes obtained by conventional interfacial polymerization methods, this spray method can be easily scaled up and consumes less aquaporin (AQP) to obtain membranes with similar performance. This demonstrates the great potential of the present method in reducing the overall cost of ABM production.

[0093] Flat-sheet ABM scale-up can be achieved by modif...

Embodiment 1B

[0094] Example 1B: Materials and chemicals

[0095] In various examples and embodiments disclosed herein, the following materials are used as non-limiting examples to demonstrate the present methods, membranes and systems.

[0096] Use 1,2-dioleoyl-sn-glycero-3-phosphorylcholine (DOPC, 20 mg / ml, Avanti Polar Lipids, Alabama, USA) as lipid to incorporate aquaporin (AQP) protein (i.e. water channel proteins). Aquaporins can be produced according to conventional methods. DOPC lipids were rehydrated using a buffered solution (phosphate-buffered saline (ie, PBS solution) with a pH of approximately 7.4). During proteolipid reconstitution, n-octyl-b-D-glucopyranoside (OG, ultrapure grade, Merck, Germany) was removed using Biobeads (Bio-rad laboratories, USA). For proteoliposome characterization, analytical grade sodium chloride (NaCl) with a purity greater than 99% was purchased from Merck (Germany) and used for stopped-flow testing. All solutions were prepared using Deionized ...

Embodiment 2A

[0098] Embodiment 2A: proteoliposome preparation

[0099] DOPC proteoliposomes can be prepared as follows.

[0100] Briefly, 10 mg of DOPC liposomes were dried by nitrogen gas and then stored in a vacuum desiccator for at least 12 hours. A PBS buffer solution containing 1 wt% OG dissolved the dried liposomes together with a certain amount of AQP. The final volume was 1 ml. The solution was freeze-thawed 3 times and extruded 21 times using a 400 nm filter (Avestin extruder, Avestin, Canada). Then, add biobeads to the solution and vortex for at least 3 h to remove OG. Finally, the solution (containing the proteoliposomes) was extruded 11 times using a 200 nm filter to obtain proteoliposomes of uniform size and distribution. Assuming that 10 mg of DOPC liposomes are present in 1 ml of the resulting proteoliposome solution, the final solution concentration is 10 mg / ml DOPC proteoliposomes.

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Abstract

Methods of fabricating a membrane comprising proteoliposomes having protein water channels are provided herein. The method may include providing a porous substrate, depositing a solution containing proteoliposomes on the porous substrate, and then contacting the porous substrate with an aqueous monomer solution and an organic monomer solution to form a selective layer on the porous substrate embedding the proteoliposomes. The method may include depositing the aqueous monomer solution, then the solution containing the proteoliposomes, then the organic monomer solution, to form the selective layer. The present disclosure also describes the membrane and a system operable to accommodate both methods.

Description

[0001] Cross References to Related Applications [0002] This application claims priority from Singapore Patent Application No. 10201900164Y filed on 8 January 2019, the entire contents of which are incorporated herein by reference for all purposes. technical field [0003] The present disclosure relates to methods of making membranes comprising proteoliposomes with protein water channels. The present disclosure also relates to such membranes, as well as systems operable to carry out the method. Background technique [0004] Fresh water shortages are worsening due to a growing population and severe environmental pollution. To produce cleaner water, reverse osmosis (RO) and nanofiltration (Nanofiltration, NF) using membranes for wastewater and / or seawater have been developed for more than 40 years. Commercial RO and NF membranes that have been developed include Thin-film composite (TFC) membranes. [0005] Typically, TFC membranes can have a layered structure where a polya...

Claims

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Application Information

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IPC IPC(8): B01D67/00B01D69/14B01D69/12B01D69/06B01D61/02C02F1/44
CPCC02F1/44B01D71/06B01D71/56B01D67/0006B01D71/74B01D69/144B01D2323/42B01D2323/26B01D69/105B01D69/1251B01D69/02B01D2325/04C08L81/06C08L2205/20
Inventor 王蓉齐萨仁李晔
Owner NANYANG TECH UNIV
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