7 results about "Proteolipids" patented technology

The application belongs to the technical field of biological medicine and functional cosmetics, and discloses acid-resistant collagen liposomes, a preparation method and application thereof. The liposome has a three-layer structure, and from inside to outside, it is sequentially a recombinant type III humanized collagen, a yolk lecithin-cholesterol lipid carrier core, and a sodium hydroxide-isopropyl alcohol system ultrasonic carboxymethylated chitosan surface modification layer. The modified chitosan has a substitution degree of 1.0-1.1 and a viscosity of 100-150 mPa·s. The preparation method is based on the film hydration method, and the liposome is prepared through basic liposome preparation, modified chitosan preparation and liposome surface modification. The freeze-dried powder can also be prepared through freeze-drying. The liposome is stable in a pH 1.0-6.0 acidic environment, can effectively protect the collagen from degradation, has antioxidant properties and no cytotoxicity, and the preparation process is easy to industrialize. The liposome can be used for preparing a pharmaceutical composition for treating digestive system diseases and repairing skin wounds, and a cosmetic composition for skin care, and has a wide application prospect.

Disclosed is a protein comprising no more than three human autoantigenic proteins, wherein a first human autoantigenic protein comprises a truncated myelin oligodendrocyte glycoprotein (MOG) amino acid sequence, a second human autoantigenic protein comprises a myelin basic protein (MBP) amino acid sequence, and a third human autoantigenic protein comprises a truncated proteolipid protein (PLP) amino acid sequence. Also disclosed are related nucleic acids, pharmaceutical compositions, methods of treating a demyelinating disease, and methods of producing the proteins.

The invention discloses a platycodon grandiflorum phosphomannose isomerase gene PgPMI as well as an encoded product and application thereof, and belongs to the technical field of platycodon grandiflorum genes. According to the invention, the platycodon grandiflorum phosphomannose isomerase gene PgPMI is cloned from platycodon grandiflorum, and the nucleotide sequence of the platycodon grandiflorum phosphomannose isomerase gene PgPMI is shown as SEQ ID NO. 1. According to the invention, the expression of the PgPMI gene in escherichia coli is successfully realized, and an enzymatic experiment verifies that the PgPMI has catalytic activity, so that mannose-6-phosphoric acid and fructose-6-phosphoric acid can be mutually converted, and a foundation is laid for revealing biosynthesis of platycodon grandiflorum polysaccharide. In addition, the platycodon grandiflorum phosphomannose isomerase can also be applied to biosynthesis pathways of plant ascorbic acid, cell walls, glycosylated proteins, lipoproteins, glycosyl phosphate inositol ankyrin and the like.

The application provides a tumor inhibiting composition and a preparation method and application thereof, and belongs to the technical field of medicines. After mesoporous carbon nanotubes are oxidized and chlorinated, oleanolic acid dithiocarbamate conjugate and (2-hydroxypropyl)-beta-cyclodextrin are coupled, drugs are loaded, a protein liposome solution is added, ultrasonic treatment is carried out, centrifugal separation is carried out, unloaded drug-loaded carbon nanotubes are removed, supernatant is freeze-dried, and the tumor inhibiting composition is prepared. The tumor inhibiting composition greatly improves the drug loading capacity of the nanosystem, the synergistic effect of multiple drugs, the anti-tumor effect, realizes the effects of targeting, slow and controlled release of drugs, improves the drug utilization rate, reduces the intake amount of drugs, reduces side effects, and improves the compliance of patients.

The present application relates to a kind of microstructure supermolecular elastin, the microstructure supermolecular elastin includes liposome and supermolecular elastin and hydrolyzed elastin wrapped inside liposome;The membrane material of the liposome includes phospholipid and polyol.The present application creatively constructs an elastin liposome, supermolecular elastin and hydrolyzed elastin are co-encapsulated in the inside of liposome, can significantly improve the skin delivery depth of elastin, realize the efficient, safe transdermal delivery of macromolecular active protein;While supermolecular elastin and hydrolyzed elastin are mutually synergistic, can deeply penetrate skin, realize the effect of moisturizing and anti-wrinkle.

The application relates to bioinformatics and metabolomics, and discloses a human tissue lipid metabolomics multidimensional statistical analysis method, equipment, medium and product. The method comprises the following steps: obtaining mass spectrometry detection original data for pretreatment to obtain a lipid quantitative data set; based on the lipid quantitative data set, a first differential metabolite candidate set is obtained; based on a significance parameter and a feature importance, a content change lipid set is determined; feature selection is performed on the content change lipid set, a lipid metabolite subset meeting a model performance evaluation index requirement is screened, and a second differential metabolite set is formed; the second differential metabolite set is respectively associated with a metabolic pathway database and a protein-lipid interaction database for correlation analysis, and a multidimensional correlation analysis result containing the second differential metabolite set, metabolic pathway information and target protein information is output, so that analysis from differential lipids to target proteins is realized.

The application belongs to the technical field of biological materials, and particularly relates to a silk fibroin-loaded hydrogel liposome with transdermal delivery effect and a preparation method and application thereof. The preparation method comprises the following steps: dissolving degummed silk in a neutral salt solution to obtain a silk fibroin solution, performing dialysis filtration to obtain a silk fibroin aqueous solution; then performing a diazo coupling reaction on the silk fibroin aqueous solution to obtain an azide-functionalized silk fibroin aqueous solution; dissolving phospholipid and cholesterol in a solvent to obtain a mixed solution; adding the azide-functionalized silk fibroin aqueous solution into the mixed solution to perform ultrasonic emulsification to obtain an emulsion; sequentially performing reverse evaporation treatment on the emulsion to obtain a gelled silk fibroin-loaded liposome; and performing freeze-drying on the gelled silk fibroin-loaded liposome to obtain the silk fibroin-loaded hydrogel liposome with transdermal delivery effect.