67 results about "Proteolipids" patented technology

The present invention generally related to a nanofabricated membrane including polymerized proteoliposomes. The nanofabricated membrane is a bio-nano fused selective membrane using protein-incorporated uv-crosslinkable liposomes with a chemical reactive biocompatible interstitial matrix. In the present invention, internally UV-crosslinked protein-incorporated proteolipsomes are used because the proteoliposomes made by natural lipids have a short life time and a weak resistance to the circumstantial stresses such as a high and low temperature, pressure, ionic strength etc. Furthermore, the proteo-vesicles made by amphiphilic block copolymers provide less consistency in accomplishing proper functionality batch to batch because of the inevitable polydiversity of the polymer.

Combination vaccines for treating or preventing Neisseria meningitidis infection are described. The vaccines include Neisseria meningitidis serogroup B proteoliposomic vesicles and Neissera meningitidis serogroup C conjugated oligosaccharides.

The invention discloses a stable system for carrying adriamycin albumin lipid and a preparation method thereof. The invention is characterized by combining the adriamycin on albumin lipid vesica through ultrasonic, high pressure isotrope or micro-fluidization technique. The invention has the advantages of both significantly improving the efficacy and stability of adriamycin lipid vesica and raising the entrapment rate of medicine in the lipid vesica.

Methods for the detection of active lupus nephritis (LN) and worsening renal disease activity and / or active LN in patients diagnosed with systemic lupus erythematosus, using a panel of biomarkers including transferrin (Tf), ceruloplasmin (Cp), alpha-1-acid glycoprotein (AGP1), lipocalin-like prostaglandin D synthetase (L-PGDS), and urinary neutrophil gelatinase associated lipocalin (UNGAL).

Methods of preparing a proteoliposome comprise the step of contacting a liposome with an effective portion of RalBP1 to create a proteoliposome. RalBP1 is effective for the protection and treatment of mammals and the environment against the accumulation of toxic compounds, and prevents accumulation of one or more toxic compounds, reduces the concentration of toxic compounds, and protects against further contamination with one or more toxic compounds. In addition, RalBP1 is effective for the protection and treatment of mammals against the effects of ionizing radiation.

The present invention is one kind of orally taken nanometer protein polypeptide composition particle with enveloping rate higher than 90 % and medicine carrying amount up to 3-10 %. The mild preparation process without high temperature, high speed shearing and other intense condition can ensure the bioactivity of the polypeptide medicine. The preparation process includes dissolving water soluble protein polypeptide medicine and amphiphilic lipid material in non-water solvent I, eliminating solvent to form protein-lipid composition, dissolving the composition and proper polymer material in non-water solvent II to coat the composition inside the polymer material through emulsified solvent diffusion in liquid phase, and volatilizing the solvent to form particles of size 10-500 nm. The present invention is suitable for preparing oral medicine preparation.

The present invention proposes formative agent of protein complex, in which a polyphenol is useful component, and the agent is useful as gene complex, cell adhesion inhibitor or immune tolerogen. The polyphenol of forming the agent is selected from catechin group consisting of epigallocatechin-gallate, tannic acids, or proanto-dianisidine, a protein of the protein complex is selected from proteins consisting of animal proteins composed of polypeptide chain of peptide-combined amino acids, vegetative proteins, nucleus proteins, glycogen proteins, lipo-proteins and metal proteins, the gene complex comprises by compositing genes by polyphenol catechins in order to introduce genes to cells of animals or human bodies, a cell composed of the cell adhesion inhibitor is selected from cells consisting of an animal cell including a stem cell, skin cell, mucosa cell, hepatocyte, islet cell, neural cell, cartilage cell, endothelial cell, epidermal cell, osteocyte or muscle cell isolated from human or animal organism, or sperm, ovum or fertilized egg of domestic animals or fishes and a tissue or an organ for transplantation of the immune tolerogen is selected from the tissue or the organ consisting of skin, blood vessel, cornea, kidney, heart, liver, umbilical cord, bowels, nerve, lung, placenta or pancreas.

Methods of preparing a proteoliposome comprise the step of contacting a liposome with an effective portion of RalBP1 to create a proteoliposome. RalBP1 is effective for the protection and treatment of mammals and the environment against the accumulation of toxic compounds, and prevents accumulation of one or more toxic compounds, reduces the concentration of toxic compounds, and protects against further contamination with one or more toxic compounds. In addition, RalBP1 is effective for the protection and treatment of mammals against the effects of ionizing radiation.

The invention relates to preparation and application of a mycoplasma hyopneumoniae multi-epitope mucosal vaccine. A mycoplasma hyopneumoniae membrane protein, an adhesive protein P97, a lipoprotein P65, a specific membrane protein P46, a B cell epitope, a Th epitope, a CTL epitope and a cholera toxin subunit B are taken as a vaccine frame structure, a pRSETA carrier is cloned in through flexible linker connection, then Escherichia coli is transformed, and fermentation, purification and preparation technologies are carried out, so that the mycoplasma hyopneumoniae multi-epitope mucosal vaccine with ideal immunogenicity is obtained. A self-made mucosal adjuvant is used in a preparation process, so that production and using processes of the vaccine are simpler and more convenient. Animal experiments show that the mycoplasma hyopneumoniae multi-epitope mucosal vaccine not only has good safety but also can stimulate effective mucosal immunity, humoral immunity and cellular immune reactions.

The invention discloses phytochemical agents derived from Holoptelea integrifolia and novel composition(s) comprising at least one component selected from the extract(s), fraction(s) and active compound(s) for the protection and alleviation of Metabolic Syndrome, insulin resistance, endothelial dysfunction, chronic kidney disease, atherosclerosis, diabetes and other disease conditions associated with metabolic syndrome. The invention also discloses the amelioration of certain biomarker molecules such as Peroxisome proliferator-activated receptor gamma (PPAR-γ), Adipose Differentiation Related Protein (ADRP), CD36, Adipocyte Fatty-acid-Binding Protein (aP2 / FABP-4 / A-FABP), beta-3 Adrenergic Receptor (β3AR), Leptin, Perilipin and Adiponectin by using the phytochemical components derived from Holoptelea integrifolia.

The invention relates to a method for directly converting plasma into hydrosulfite and application thereof. The method provided by the invention, in comparison with a similar hydrosulfite converted product on the market, has the greatest advantages that free DNA (Deoxyribonucleic Acid) of the plasma does not need to be extracted, the plasma can be directly used as a hydrosulfite conversion sample,and not only is the time of extracting the free DNA saved, but also the loss of the free DNA in an extraction process is avoided. Secondly, according to the method provided by the invention, in orderto protect the DNA from being influenced by miscellaneous protein, lipid and the like, a protective agent is added into a hydrosulfite conversion system. In addition, in order to further improve therecovery rate of the free DNA, the method adopts a high-sensitivity magnetic bead to carry out recovery. The entire conversion process does not exceed 2 hours; the centrifugal operation is not involved; the automation is more easily realized, and the method provided by the invention has the advantages of being low in cost, simple and convenient to operate, high in conversion ratio, high-purity inobtained DNA and the like.

The invention discloses a preparation method of artificial liposome, belongs to the field of pharmacy, and relates to a preparation method of protein medicament liposome. In the preparation method, nano spherical grain yolk lecithin zirconium or soya bean lecithin zirconium is used as a carrier, and balsam pear protein MAP30, fleece-flower root protein GAP31, lipase CRL or bovine serum albumin (BSA) is used as a wrapping protein. The preparation method comprises the steps of: adding phosphate buffer solution to the carrier material, stirring under the conditions of the temperature being 4 DEG C and rotation rate being 3500rpm to obtain a colorless and transparent dispersion, dissolving the wrapping protein in a phosphate buffer solution, then slowly dropwise adding to the dispersion, quickly stirring in the low-temperature condition to form stable liposome, centrifugally separating out protein, repeatedly washing, centrifuging, and freeze-drying at low temperature to obtain a finished product. The method has the characteristics of simple process, mild condition, high repeatability, high protein adsorption capacity and stable liposome structure.

The invention discloses a lipid-protein edible double-layer active membrane and a preparation method thereof. The preparation method comprises the following steps: respectively dissolving an alcohol-soluble protein and a water-soluble protein in an acetic acid-water solution, and adding a hydrophobic active substance into the alcohol-soluble protein and / or adding a hydrophilic active substance into the water-soluble protein to respectively obtain an alcohol-soluble protein solution and a water-soluble protein solution; uniformly mixing the alcohol-soluble protein solution with the water-soluble protein solution, and adding a plasticizer; and mixing the film forming solution with solid lipid, heating to a temperature being equal to or higher than the melting point of the lipid, carrying outhigh-speed shearing homogenization to form an emulsion, and casting to form the membrane. The preparation method is simple and convenient, and the protein-lipid double-layer active membrane can be prepared through one-time tape casting, so the drying time can be shortened, and the production efficiency can be improved. The active membrane has a good one-way water blocking effect and good mechanical properties, and the release rate of an active matter can be regulated by regulating the ratio of the alcohol-soluble protein to the water-soluble protein and the mass percentage of the lipid.

Hair treatment compositions include—based in each case on the weight thereof—0.0001% to 10% by weight at least one oligopeptide having at least one Glu-Glu-Glu amino acid sequence, wherein the amino group may be free or protonated and the carboxyl groups may be free or deprotonated, and 0.0001% to 10% by weight at least one proteolipid of the formula (I) in which R1 is a straight-chain hydrocarbyl radical having 8 to 22 carbon atoms, R2 is (—CH2)y—CH3, with y=0 to 22, Y is —H or —OH, X− is a physiologically acceptable anion, Hy is an oligopeptide residue from keratin hydrolysate, and x has values from 0 to 22. In these compositions, proteolipids and oligopeptides have mutually enhancing positive effects on the hair and the hair follicle.

The invention belongs to the field of biomedicine and cosmetics, and specifically relates to the technical field of liposome preparation, in particular to a preparation method of animal blood fibronectin liposome and application of the obtained product. In the invention, animal blood fibronectin is added to hydrogenated lecithin, cholesterol and other materials to make protein liposome FNL. The fibronectin encapsulated by the liposome is applied to skin care products and can be absorbed by the skin more quickly. After absorption through the skin, multiple effects of whitening, anti-aging, moisturizing, repairing, etc. can be better achieved, thus showing a good application prospect for fibronectin to enter high-end cosmetics.

The invention discloses a method for screening an effective shRNA of a lipoprotein lipase gene, which comprises the following steps of: A1, designing and synthesizing the singe-chain DNA oligonucleotides fragment for coding target shRNA; A2, constructing a carrier of pENTR / CMV-GFP / U6-shRNA; A3: constructing a carrier of pDsRed1-C1-LPL; and A4, screening effective sequences of the shRNA. The interference carrier and target gene are marked by using red fluorescent light and green fluorescent light respectively, so that the screening process of interference sequences is time-saving and efficient, the result is more intuitionistic and more reliable, and a plurality of sequences can be screened simultaneously.

The present invention is a hair treatment agent comprising at least one surfactant and at least one proteolipid of the formula R′—X—R″, wherein R′ is a straight-chain or branched, saturated or unsaturated hydrocarbon radical having 11 to 24 carbon atoms; R″ is a protein, a peptide, or a protein hydrolyzate; X is —C(O)O—, —N+(RIII2)RIV—, or —N(RIII)RIV—; RIII is —(CH2)x—CH3— with x=0-22; and RIV is —CH2—CH(OH)—CH2— or —(CH2)x— with x=0-22; with the proviso that R″ consists of keratin or a keratin hydrolyzate when X is —C(O)O—. The agents of the present invention improve many properties of hair such as giving enhanced shine and impregnation of hair.

The present invention belongs to the technical field of nutritious foods, and especially relates to a rectal cancer nutritious food and a preparation method thereof. The rectal cancer nutritious foodcontains nutrient elements including proteins, fat, carbohydrate, vitamins, minerals, ginkgo flavonoids and phytosterol. The nutritious food provided by the invention can provide sufficient and comprehensive nutrients for the organism, more importantly, the food enhances the barrier function of intestinal mucosa, better accords with the metabolic physiological state, facilitates the protein synthesis and metabolism regulation of the organism, promotes the recovery of intestinal function and form, prevents bacteria and toxin translocation, obviously reduces the occurrence of intestinal source infection, and is an effective way to improve the nutritional status of chemotherapy patients. The food has a certain improvement effect on the nutritional status of chemotherapy patients with middle or advanced rectal cancer, can reduce adverse reaction of patients, and can effectively improve the immunity of patients.

The invention belongs to the technical field of cosmetics, and particularly relates to hydrolyzed protein lipidosome as well as a preparation method and application thereof. The hydrolyzed protein lipidosome comprises the following components of cosmetic active components, lipidosome framework components, object components and a freeze-drying protective agent, wherein the cosmetic active components include hydrolyzed keratin and ceramide; the liposome framework components include sterol and phospholipid; and the object components include a surfactant, an antioxidant, a thickener and essence. The lipidosome is used as a carrier of the hydrolyzed keratin, so that the stability of the hydrolyzed keratin can be improved on one hand, and the transdermal absorption of the hydrolyzed keratin canbe promoted on the other hand. Meanwhile, the concentration of active substances is continuously increased in a process of being in contact with the skin, so that the active substances act on cells more sufficiently, the moisture and elasticity of the skin are increased, and the moisturizing performance is high.

The invention describes materials useful for calibrating methods, and standards, calibration verification, linearity, and quality control materials for any method used to detect lipids and / or lipoproteins. This invention describes methods for producing, and compositions produced thereby, stable lipid controls, standards, and reagents including a stabilizing amount of TDPA, and further including a known amount of a substantially pure constituent of interest, including total cholesterol (CHOL), triglycerides (TRIG), high density lipoprotein (HDL), APO lipoprotein A (APO-A), APO Lipoprotein B (APO-B), low density lipoprotein (LDL), apolipoprotein a Lp(a) and other lipoprotein moieties useful for calibrating, standardizing, verifying, quality control, and the like, relating to use of an instrument for assessing the level of such constituents in a sample.

Methods of detecting ZNT8 antibodies in serum are described. The methods include proteoliposomes comprising a transmembrane domain (TMD) and a cytosolic domain (CTD) of ZnT8 proteins exposed on the exterior of the proteoliposome; serum comprising antibodies targeting the ZnT8 proteins; and labelled captured autoantibodies that bind to ZnT8 antibodies.

The invention provides low-immunogenicity fish skin collagen and a preparation method thereof. The preparation method comprises the following steps of by taking fish skin as a raw material, pretreating the fish skin, removing impurities and degreasing, coarsely extracting collagen, removing terminal peptide, refining and drying the collagen, inactivating endotoxin and the like, thereby obtaining the collagen with obviously reduced contents of impure protein, fat, terminal peptide and endotoxin. In the fish skin pretreatment stage, hydrogen peroxide and sodium hydroxide are used for treatment, and the liquid is changed at a certain time point, so that the fish skin pretreatment effect is better; in the stages of extraction and further immunogen removal, acetic acid and protease are used for enzyme digestion terminal peptide treatment, and after a collagen finished product is obtained through refining and freeze-drying, endotoxin is inactivated through irradiation. The extraction method is simple, the extraction efficiency is relatively high, the obtained collagen immunogen substance is low in content and relatively high in purity, the adverse reaction of the collagen in the medical process is effectively reduced, and the application field of the collagen is further expanded.

The invention relates to a process for preparing saposin lipoprotein particles, comprising a saposin-like protein, lipids and optionally a hydrophobic agent wherein the saposin-like protein or the hydrophobic agent is selectively bound to a support to allow the self-assembly of the saposin lipoprotein particles. The process of the invention comprises the step of a.) providing the hydrophobic agent and lipids, b. 1) / b.2) contacting the hydrophobic agent or the saposin-like protein with a support that is capable of selectively binding either of the two molecules to the support, c.1) / c.2) contacting the support-bound particle components with the remaining particle components, either the saposin-like protein or the hydrophobic agent, to allow fpr the self-assembly of the saposin lipoprotein particle on the support and d.) optionally eluting the support-bound saposin lipoprotein particles.

The present invention belongs to the technical field of nutritious foods, and especially relates to a colon cancer nutritious food and a preparation method thereof. The colon cancer nutritious food contains nutrient elements including proteins, fat, carbohydrate, vitamins, minerals, amino acidss, ginkgo flavonoids and phytosterol. The nutritious food provided by the invention can provide sufficient and comprehensive nutrients for the organism, more importantly, the food enhances the barrier function of intestinal mucosa, better accords with the metabolic physiological state, facilitates the protein synthesis and metabolism regulation of the organism, promotes the recovery of intestinal function and form, prevents bacteria and toxin translocation, obviously reduces the occurrence of intestinal source infection, and is an effective way to improve the nutritional status of chemotherapy patients. The food has a certain improvement effect on the nutritional status of chemotherapy patients with middle or advanced colon cancer, can reduce adverse reaction of patients, and can effectively improve the immunity of patients.

The invention relates to balneological preparations, especially bath and shower preparations, which, as a pourable or liquid premixture concentrate, contain liposome-forming components, such as phospholipids, in addition to natural and / or synthetic lipids, and are also provided as mixtures containing primarily vegetable proteins, surfactants, and optional additives. When entering in contact with an excess of water, the stable systems directly form skin-active proteoliposomes having a catalytic activity for enhancing transfer of active ingredients into the skin. The stable systems can be used directly or in diluted or solid forms of application (such as lipid-containing proteasome shower preparation, proteasome bath powder, and the like).

The present invention describes the synthesis of biological samples that can be used for the purpose of enhancing the signal-to-noise ratios achievable during the imaging of protein synthesis, amino acid transport and neurotransmitter transport, particularly in applications where single-molecule resolution is demanded. The present invention provides quencher-labeled elongation factor (EF-Tu) and fluorophore-labeled tRNA. When these molecules are present in a ternary complex with GTP, the fluorescently-labeled tRNA is quantitatively quenched. Once the tRNA is incorporated into an actively translating ribosome, however, a burst of fluorescence is released and can be detected by a variety of techniques, including smFRET imaging. The invention further provides novel EF-Tu constructs for achieving quencher labeling at high levels while retaining native or near native activity in the translation reactions, as well as methods for preparing stable ternary complexes, methods of protein sequencing, methods of detecting amino acid transport using a proteoliposome assay system and the proteoliposomes systems and methods of imaging translation events in single living cells. The present invention should have an immediate impact on next-generation sequencing technologies and the detection of neurotransmitter transporter activities in both in vitro and in vivo settings, a critical component of drug activity / screening assays targeting this important class of molecules.

The invention discloses a method to prepare proteoliposomes using glycerol or polyethylene glycols (PEG) in the rehydration step. The method eliminates the use of expensive surfactants and subsequent time-consuming removal of those surfactants during the preparation of proteoliposomes. The fusible proteoliposome reconstituted with phage portal proteins or other hydrophobic channel proteins are useful for nanopore sensing technology, including ultrafast DNA sequencing and biomedical diagnostic applications.

This invention relates to the design and construction of a gene encoding an encephalogenic epitope of proteolipid protein (PLP), design and construction of a gene encoding an encephalogenic epitope of myelin based protein (MBP), to methods of expression of a PLP epitope, to methods of expression of a MBP epitope, to methods of in vivo secretion of a PLP epitope, and to methods of transferring the partial PLP gene to a host to ameliorate the progression of an immune response to self antigens derived from myelin proteins, to methods of in vivo secretion of a MBP epitope, and to methods of transferring the partial MBP gene to a host to ameliorate the progression of an immune response to self antigens derived from myelin proteins.