Acid-resistant collagen liposomes, and methods of making and using same

The three-layer structure of acid-resistant collagen liposomes solves the stability and release problems of recombinant human collagen in acidic environments, achieving stable encapsulation and slow release in strongly acidic environments. It has antioxidant activity and good biocompatibility, and is suitable for the treatment and care of digestive system diseases and skin wounds.

CN122440495APending Publication Date: 2026-07-24ANHUI SHIYU BIOTECHNOLOGY CO LTD +2
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Patent Information

Application Number
CN202610388581.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-27
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Recombinant humanized collagen is structurally unstable and prone to denaturation and inactivation in acidic environments. Traditional liposomes have unstable membrane structures in strongly acidic environments. Existing chitosan-modified liposomes are unstable under extremely acidic conditions. There is a lack of liposome delivery systems that can balance strong acid stability, high encapsulation efficiency, controllable release, and good biocompatibility.

Method used

Acid-resistant collagen liposomes with a three-layer structure consist of a lipid bilayer composed of recombinant humanized collagen, egg yolk lecithin, and cholesterol, and an ultrasonically carboxymethylated chitosan modification layer in a sodium hydroxide-isopropanol system. Stable liposomes are prepared by thin-film hydration and a one-step chitosan modification process.

Benefits of technology

It remains stable in acidic environments with a pH range of 1.0–6.0, achieving low release rates and high encapsulation rates. It possesses antioxidant activity and good biocompatibility, making it suitable for bioactive delivery in various acidic environments.

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Abstract

The application belongs to the technical field of biological medicine and functional cosmetics, and discloses acid-resistant collagen liposomes, a preparation method and application thereof. The liposome has a three-layer structure, and from inside to outside, it is sequentially a recombinant type III humanized collagen, a yolk lecithin-cholesterol lipid carrier core, and a sodium hydroxide-isopropyl alcohol system ultrasonic carboxymethylated chitosan surface modification layer. The modified chitosan has a substitution degree of 1.0-1.1 and a viscosity of 100-150 mPa·s. The preparation method is based on the film hydration method, and the liposome is prepared through basic liposome preparation, modified chitosan preparation and liposome surface modification. The freeze-dried powder can also be prepared through freeze-drying. The liposome is stable in a pH 1.0-6.0 acidic environment, can effectively protect the collagen from degradation, has antioxidant properties and no cytotoxicity, and the preparation process is easy to industrialize. The liposome can be used for preparing a pharmaceutical composition for treating digestive system diseases and repairing skin wounds, and a cosmetic composition for skin care, and has a wide application prospect.
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Citation Information

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