175 results about "Active protein" patented technology

The invention discloses a composite double-protein hypoglycemic peptide as well as a preparation method and application thereof, and belongs to the technical field of animal and plant source double-protein active peptides. The method comprises the following steps: performing computer simulation enzyme digestion and virtual screening by utilizing bioinformatics to determine a protein raw material and protease, performing enzymolysis on animal and plant double proteins (soybean protein and casein) serving as raw materials by adopting neutral protease, and performing separation, purification and structural identification, thereby obtaining the protein. The protein peptide fragment with high alpha-glucosidase inhibitory activity is obtained by virtually screening molecular docking, and the amino acid sequences of the protein peptide fragment are shown as SEQ ID No: 6, SEQ ID No: 9 and SEQ ID No: 24. The compound double-protein peptide sequence provided by the invention has an inhibiting effect on the activity of alpha-glucosidase, can be used for preventing and treating diabetes mellitus, and can be used for long-term health care or treatment of people with impaired glucose tolerance or diabetics as a functional food ingredient.

The invention relates to the field of preparation of materials for articular cartilage repair, in particular to collagen and chitosan cross-linked temperature-sensitive gel for articular cartilage repair and a preparation method of the collagen and chitosan cross-linked temperature-sensitive gel. The cartilage repair material prepared by the invention adopts the recombinant human collagen with a triple helix structure as a main raw material. The raw material is prepared by using a mammalian cell expression system, is close to protein folding and polymerization of natural protein, and has a spatial structure and modification necessary for active protein. The recombinant human collagen with the triple-helix structure is good in biocompatibility and free of rejection reaction and anaphylactic reaction, and the risk of disease transmission possibly caused by animal-derived collagen can be avoided. The injection type temperature-sensitive gel is liquid at low temperature and is converted into hydrogel at 37 DEG C, so that the injection type temperature-sensitive gel is suitable for being used as a transfer carrier for in-vivo injection treatment, secondary injury of an organism is effectively avoided, and the injection type temperature-sensitive gel has a wide clinical application prospect.

The invention provides a probiotic composition for improving immunity and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: fermenting selenium-enriched nostoc sphaeroides through selenium-enriched saccharomycetes to extract selenium-enriched nostoc sphaeroides polysaccharide and selenium-enriched nostoc sphaeroides protein peptide, mixing with traditional Chinese medicine polysaccharide obtained by extracting astragalus membranaceus, rhodiola rosea, codonopsis pilosula and pseudo-ginseng, and reacting with ferric salt to prepare a polysaccharide-Fe / selenium compound; solid residues are mixed and fermented, and active protein peptide is prepared from the solid residues and selenium-rich nostoc sphaeroides protein peptide; the probiotic composition capable of improving immunity is prepared by extracting a polysaccharide-Fe / selenium compound and a polysaccharide-Fe / selenium compound through an organic solvent, extracting a permeate through an organic solvent to obtain an active component, collecting a bacterial liquid, mixing and embedding the bacterial liquid in the polysaccharide-Fe / selenium compound and sodium alginate, and uniformly mixing the bacterial liquid with the active protein peptide, the active component, taurine and ginsenoside Rg3, so that the probiotic composition capable of improving immunity can play good anti-inflammatory, anti-oxidation, immunoregulation, anti-tumor, anti-virus and other roles, and has a good application prospect. Wide application prospects are realized.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

A dual function protein is disclosed. The dual function protein may be prepared by linking a biologically active protein and an FGF mutant protein to an Fc region of an immunoglobulin. The dual function protein has improved pharmacological efficacy, in vivo duration and protein stability. The dual function protein exhibits improved pharmacological efficacy, in vivo duration and protein stability. A pharmaceutical composition containing the dual function protein as an active ingredient may be effectively used as a therapeutic agent for diabetes, obesity, dyslipidemia, metabolic syndrome, non-alcoholic fatty liver diseases, non-alcoholic steatohepatitis or cardiovascular diseases.

The invention discloses a river crab-snail breeding method based on space-time isolation and micro-flowing water circulation, and relates to the technical field of aquaculture, the key points of the technical scheme are that an isolation barrier is arranged in a river crab breeding pond, the river crab breeding pond is divided into a snail breeding area and a river crab main breeding area, and a water passing structure is arranged on the isolation barrier; a three-dimensional inhabiting base frame is arranged in the snail breeding area; generating directional water flow by utilizing oxygenation equipment to drive water in the main river crab breeding area to flow back after being filtered and purified by the snail breeding area; seed snails are put into the snail breeding area, and the snails grow and breed by feeding nutrient substances in the water body of the main river crab breeding area; collecting finished snails from the snail breeding area regularly and transferring the finished snails to the main river crab breeding area for feeding of the river crabs; in the river crab fattening period, the snail breeding area is opened, and the river crabs are allowed to freely eat the remaining snails. By means of the method, it can be guaranteed that snail populations rapidly breed to form a high-density biological filter bed, high-quality active protein bait can be provided for river crabs, and the win-win situation of water purification and feed cost reduction is achieved.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

A logic-gated protein device in which proximity-gated protein trans-splicing governs formation of an active protein from two otherwise inactive fragments.

To provide a method for inducing an immune response against a tumor-associated antigen in a subject.SOLUTION: A method comprises administering to a subject a CMV vector encoding a tumor-associated antigen in an amount effective to induce a CD8+ T cell response against the tumor-associated antigen, where the CMV vector does not express an active UL128 protein, an active UL130 protein, an active UL146 protein, or an active UL147 protein or respective orthologs thereof, and where the tumor-associated antigen comprises a specific amino acid sequence of 15 amino acid residues (MHC-E or MHC-II supertope peptide).SELECTED DRAWING: None

The present invention provides targeting polyplexes comprised of (i) nucleic acids, in particular nucleic acids encoding pharmaceutically active peptides or proteins, and (ii) targeting conjugates comprising LPEI and PEG fragments that are connected by discrete linkages formed through defined, chemoselective reactions. Thus, the LPEI fragment is bonded in a linear end- to-end fashion to a single PEG fragment. The linear conjugates are further conjugated to a targeting fragment to enable selective interaction with a particular cell type. The polyplexes selectively deliver the nucleic acids to the targeted cells resulting in high expression and efficient protein translation as well as secretion of the encoded pharmaceutically active proteins.

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The application provides a mass spectrometry detection device and method for active protein-small molecule interaction, which uses a mass spectrometry sensor to obtain tandem mass spectrometry data of non-covalent complexes of active protein incubated with small molecule compounds at different concentration points; performs non-linear curve fitting on the concentration sequence of the small molecule compounds based on the ion combination rate of the protein binding small molecules at each concentration point, to obtain an apparent dissociation constant of the active protein-small molecule interaction; determines a local conformation change parameter of the active protein induced by small molecule binding and a potential binding domain of the small molecule; and performs fusion evaluation on the binding strength and conformation influence degree of the protein-small molecule interaction based on the apparent dissociation constant, the local conformation change parameter and the potential binding domain, to output a stability grade of the protein-small molecule interaction. Based on the above scheme, non-denatured state mass spectrometry detection of protein-small molecule complexes in a liquid phase environment can be realized.

The application provides high-protein long-shelf-life milk and a preparation method thereof. The preparation method comprises a step-by-step washing and filtering process, a continuous process without buffer storage, and a combination of ceramic membranes, centrifugal sterilization and pasteurization, prevents the loss of calcium ions in the concentration process, adjusts the lactose content to improve the taste, ensures high protein content, high active protein content, rich and sweet taste of the product, and improves the safety of the milk. Even in a more stringent 10-15 DEG C storage environment, the shelf life can reach 15 days.

Disclosed herein is a stable apo (ligand-free)-active complex of a G protein coupled receptor (GPCR) and a G protein complex, wherein the G protein complex is a cognate heterotrimeric G protein comprising an alpha subunit (Gα), a beta subunit (Gβ) and a gamma subunit (Gγ). The apo-active GPCR-G protein complex can be isolated from a cell membrane in a purified form and maintains its stability in the purified form.

This invention discloses a fermented feed for improving the natural antibacterial and antioxidant properties of duck eggs. The fermented feed is prepared through a two-stage fermentation process using a fermentation substrate comprising the following components in parts by weight: 35-60 parts corn, 15-30 parts soybean meal, 5-15 parts wheat bran, 4-10 parts rice bran, 5-9 parts extruded flaxseed, 2-6 parts grape seed meal, 1-4 parts moringa flavonoid powder, 0.5-2.5 parts dicalcium phosphate, 0.5-3 parts limestone powder, 0.1-0.5 parts salt, and 0.1-0.8 parts compound premix. The two-stage fermentation involves first inoculating with Bacillus for aerobic fermentation, followed by inoculation with lactic acid bacteria and the addition of chitosan oligosaccharides for anaerobic fermentation. This invention significantly increases the content of active proteins such as lysozyme and ovotransferrin in duck egg white, enhancing its antibacterial properties and antioxidant capacity, and providing an efficient and economical technical path for the development of highly active duck egg white raw materials.

This invention relates to the field of biomaterials, specifically to the application of an acidic hydrolysate of a giant salamander mucus extract in the preparation of a root surface treatment agent. The root surface treatment agent comprises a product obtained by reducing lyophilized powder of salamander skin secretions with an acidic solution at a concentration of 0.2-0.3 mol / L and a pH of 1-5, wherein the mass ratio of the lyophilized salamander skin secretions to the acidic solution is (1-3):1; the resulting root surface treatment agent, after adjustment with an alkaline solution, has a pH of 6-6.5. This root surface treatment agent demonstrates significant potential in promoting natural tooth replantation and restoration, with effects including promoting early load stabilization through natural wet bonding, assisting in the construction of a regenerative microenvironment through active proteins, and promoting adhesion between periodontal ligament stem cells and the matrix by enhancing adhesion signal expression.

The invention relates to the technical field of biological medicine, in particular to an exosome medicine preparation for treating knee osteoarthritis and a preparation method thereof, and the exosome medicine preparation is characterized in that an exosome is derived from mesenchymal stem cells subjected to three-dimensional culture in a first hydrogel matrix containing sodium alginate and hyaluronic acid; flurbiprofen or a pharmaceutically acceptable salt or ester thereof; and a pharmaceutically acceptable second gel matrix. Sodium alginate and hyaluronic acid composite hydrogel serves as a three-dimensional culture carrier of mesenchymal stem cells, a joint microenvironment is simulated to induce stem cells to secrete high-activity exosomes rich in key functional nucleic acid and active protein, meanwhile, the exosomes are scientifically compounded with flurbiprofen, and synergistic mechanisms of anti-inflammatory analgesia and tissue repair are achieved; and a local slow-release delivery system is constructed through the second gel matrix, so that long-acting stable release of active ingredients is realized, the systemic side effect of the medicine is reduced, the bioavailability of the exosome is improved, and the problems of obvious side effect, limited repair effect and the like in the prior art are solved.

This invention relates to a botulinum toxin A1 precursor molecule, a nucleic acid molecule, an engineered bacterium, a method for preparing the same, and a method for preparing natural botulinum toxin A using the precursor molecule. The precursor molecule comprises a light chain and a heavy chain, and a linker region connecting the light and heavy chains. The amino acid sequence of the precursor molecule is shown in SEQ ID NO. 4. The light chain is the amino acid sequence from position 1 to position 444 of natural botulinum toxin A, as shown in SEQ ID NO. 5. The heavy chain sequence is the amino acid sequence from position 449 to position 1296 of natural botulinum toxin A, as shown in SEQ ID NO. 6. The amino acid sequence of the linker region is shown in SEQ ID NO. 11. This invention provides a safe process, and the resulting recombinant botulinum toxin not only has a sequence identical to the natural botulinum toxin active protein but also exhibits high activity, providing a low-cost, high-activity botulinum toxin product for clinical use.

The present invention provides a class of drug delivery systems for VHL-based protein degradation agents. Specifically, the invention provides a conjugate or a pharmaceutically acceptable salt thereof, and the conjugate is a conjugate using the drug delivery system as shown in formula (I). The conjugate provided by the invention can deliver the protein degradation agent to a target position in a targeted manner and release the active protein degradation agent.

The present invention provides a recombinant sST2 protein, the amino acid sequence of the recombinant sST2 protein is shown in SEQ ID NO.3 or SEQ ID NO.5. Among them, the nucleotide sequence encoding the amino acid shown in SEQ ID NO.3 is shown in SEQ ID NO.4, and the nucleotide sequence encoding the amino acid shown in SEQ ID NO.5 is shown in SEQ ID NO.6. The recombinant sST2 protein prepared by the present invention has good performance, and the modified recombinant protein has a higher potency, which is better than the recombinant protein of the original sequence. The purity of the recombinant protein is ≥95%, and the activity is ≥95%; the protein stability is better, and it can be stored for one year at ‑20°C before reconstitution, and can be stored for more than 3 months at ‑20°C after reconstitution; the recombinant protein is close to the natural protein and has good interoperability with clinical samples. The recombinant proteins prepared by the present invention can be used for the preparation of calibrators and quality control products in the target detection kit, and can also be used for related experiments such as drug targeted analysis and drug development.

The invention relates to the technical field of dairy product processing, in particular to Chinese yam milk containing active protein and a preparation method thereof, and the Chinese yam milk comprises the following raw material components: raw milk, Chinese yam powder, gellan gum, sucrose fatty acid ester, glyceryl monostearate, glyceryl distearate, amylase, glucoamylase, food essence and water. According to the method, the Chinese yam and the milk are combined, a sweetening agent is not additionally added, meanwhile, the beverage is endowed with a sweet feeling, meanwhile, the damage of heat treatment to active protein in cow milk in the processing process is overcome, and the Chinese yam milk which is good in flavor and stable in system and contains the active protein is prepared.

The invention discloses an inhalation type transpulmonary alveolar drug delivery vesicle and a preparation method and application thereof.The inhalation type transpulmonary alveolar drug delivery vesicle comprises an outer layer lipidosome and a drug core, the lipidosome is of a bilayer structure composed of phospholipid and cholesterol, the outer surface of the lipidosome is modified by polyethylene glycol-surfactant protein A with an enzyme response function, the drug core wraps the alveolar surface active substance. According to the invention, PEG-(SP-A) is adopted as an enzyme-responsive part to modify the outer surface of the liposome, the drug delivery vesicle can be efficiently disintegrated after reaching the pulmonary alveoli, the drug contained in the vesicle can realize general uniform distribution of the pulmonary alveoli, a gas-liquid barrier is prevented from being blocked by the liposome which cannot be disintegrated, and the drug delivery vesicle can be applied to the pulmonary alveoli. And the condition that the liposome carries drugs to transfect and enter respiratory epithelial cells to cause potential toxicity is avoided, and an effective treatment way is provided for the lung dysplasia of the premature infant.

The present invention relates to a high-temperature frozen soil foundation reinforcement method based on ice nucleation-active bacteria. Existing frozen soil foundation reinforcement measures have problems such as insufficient performance and pollution of frozen soil resources. The present invention prepares a culture medium; utilizes the culture medium to culture ice nucleation-active bacteria; obtains an ice nucleation-active bacteria solution; injects the ice nucleation-active bacteria solution into the high-temperature frozen soil in the high-temperature frozen soil foundation area that needs to be reinforced; the ice nucleation-active bacteria grow and multiply in the high-temperature frozen soil, and when their number reaches a certain concentration in the reinforcement area, the ice nucleation-active protein anchored in its cell outer membrane can increase the freezing point of unfrozen water in the high-temperature frozen soil, and the ice nucleation-active bacteria will promote the formation of ice crystals at the corresponding high-temperature frozen soil temperature, thereby reinforcing the high-temperature frozen soil foundation. The present invention utilizes ice nucleation-active bacteria to reinforce the high-temperature frozen soil foundation, which is green, environmentally friendly, sustainable, not restricted by seasonal changes, has little disturbance to the frozen soil, and can prevent the invasion of foreign organisms from causing local biological pollution.

The disclosure is in the field of immunology and oncology, especially for treating, preventing, or inhibiting proliferative diseases, particularly cancer, infectious diseases and disorders associated with dysfunction of TNF cytokines. The disclosure relates to a novel SPD-TNFSF fusion protein including a TNF-superfamily (TNFSF) ligand, or receptor binding domain thereof, fused to a coiled-coil domain of surfactant protein-D (SPD). Also provided a trimeric or multimeric fusion protein including a plurality of SPD-TNFSF fusion proteins. The disclosure also provides an expression vector as mRNA, plasmid or virus including an isolated nucleotide sequence encoding the SPD-TNFSF fusion protein and a cell or a pharmaceutical composition including thereof.

Embodiments of the present disclosure relate generally to compositions and methods for the design of remote controlled biological systems, and more specifically to synthetic bioswitches that provide the ability to non-invasively and remotely control the function and activity of live cells, such as for example and not limitation, the expression of biologically active proteins or biological therapeutics, and the manipulation of physiologic or genetic processes and / or protein expression in live cells, in vivo (including, e.g., at desired anatomical sites) or ex vivo.

The present disclosure provides the use of catalase or a variant thereof or a nucleic acid sequence or a carrier or a delivery system in the preparation of a pharmaceutical or cosmetic composition for ameliorating an inflammatory condition of photoaged skin.

The invention provides a method for enriching Tcm cells and application thereof, and particularly, the method comprises the step of performing first co-culture on a T cell population and stem cell exosomes. The exosome secreted by the stem cells contains a plurality of active proteins, RNA, lipids, metabolites and other molecules for keeping the dryness of the cells, the stem cell exosome and the T cell population are co-cultured, and the stem cell exosome can keep the rejuvenation of the T cells in the culture process and induce the differentiation of the T cells to the Tcm cells, so that the proportion of the Tcm cells is increased, and the cell proliferation rate is increased. High cell viability and proliferation capacity are maintained, and guarantee is provided for the function exertion of the T cells and related cell products thereof.

The invention provides cytosine deaminase AiSddA, a base editor containing the same and application of the cytosine deaminase AiSddA, the amino acid sequence of the cytosine deaminase AiSddA is shown in an attached table, unreported SddA is found near a branch of a high-activity protein, the cytosine deaminase AiSddA serves as a candidate protein to be further subjected to structure comparison, and the cytosine deaminase AiSddA is obtained. According to the present invention, the CBE (Cellular Beam Element) containing the AiSddA has characteristics of high activity and small size, the SddA from Actinokineospora iranica is selected as one of the final candidate cytosine deaminase for activity evaluation based on the structure comparison result, the editing efficiency of the AiSddA at the four nuclear target sites in the HEK293T cell is detected, and the efficient C-T editing efficiency is displayed at the target position, such that the CBE containing the AiSddA has advantages of high activity, small size and the like, and can be used for the preparation of the CBE. Compared with other existing CBE, the method has obvious advantages.

The application discloses a method for collecting active proteins of water leeches, a detection method, an analysis method and application thereof, and relates to the technical field of biological medicines. The application provides the water leech seedlings treated by hunger and the living host treated by emptying, so that the water leech seedlings adsorb and suck the host, and then the active proteins are injected into the body of the host; the sample of the host after being sucked is collected, and then is made into a freeze-dried powder after being frozen and dried, so that the active proteins of the water leeches are obtained; the proteins are extracted from the freeze-dried powder, and then are subjected to enzymolysis treatment to generate peptide segments; the liquid chromatography-mass spectrometry technology is used to detect the peptide segments; and the mass spectrometry data are subjected to database searching analysis based on the protein sequence database of the water leeches and the host, so that the active proteins from the water leeches are identified and the relative content is determined. The application utilizes the characteristic that the active proteins are injected into the body of the host in the sucking process of the water leeches, and realizes efficient enrichment of the target proteins. In combination with the proteomics technology, high-throughput and high-sensitivity protein identification is realized.