261 results about "Active protein" patented technology

The invention relates to the technical field of dairy products, and particularly discloses a desalted whey product as well as a preparation method and application thereof. The method for preparing the desalted whey product comprises the following steps: (1) carrying out nanofiltration by taking natural whey or degreased sweet whey as a raw material to obtain nanofiltration trapped fluid with the dry matter content of 18-32 g / 100g; the pore diameter of the nanofiltration membrane is 200 Da to 1000 Da; (2) adjusting the pH value of the nanofiltration trapped fluid to 8-9 to obtain whey fluid after pH adjustment; and (3) performing electrodialysis on the whey liquid subjected to pH regulation to obtain desalted whey liquid. According to the preparation method disclosed by the invention, the loss rate of active protein in the raw materials can be reduced, so that the utilization rate of the raw materials is improved, and the obtained product is low in furosine content, high in proportion of undenatured protein and good in bioavailability.

The invention discloses an antistatic wig hairline and a preparation method thereof. The antistatic wig hairline is characterized by comprising regenerated polyester, a quantum dot-carbon nanotube composite antistatic agent, a phase-change flame-retardant microcapsule, a bioactive protein fiber reinforcement and a functional additive which are subjected to melt spinning to prepare the hairline, wherein the regenerated polyester, the quantum dot-carbon nanotube composite antistatic agent, the phase-change flame-retardant microcapsule, the bioactive protein fiber reinforcement and the functional additive are subjected to melt spinning. Wherein the antistatic agent is formed by compounding cadmium sulfide quantum dots and carboxylated single-walled carbon nanotubes, and has good stability; the phase-change flame-retardant microcapsules can enhance the flame retardance; the bioactive protein fiber reinforcement is composed of dopamine modified mulberry silk fibroin fibers. The invention solves the problem that the anti-static property of the wig is reduced after the wig is used for a long time and cleaned, improves the anti-static property, flame-retardant property and the like of the wig, and has higher practical value.

The invention discloses a composite double-protein hypoglycemic peptide as well as a preparation method and application thereof, and belongs to the technical field of animal and plant source double-protein active peptides. The method comprises the following steps: performing computer simulation enzyme digestion and virtual screening by utilizing bioinformatics to determine a protein raw material and protease, performing enzymolysis on animal and plant double proteins (soybean protein and casein) serving as raw materials by adopting neutral protease, and performing separation, purification and structural identification, thereby obtaining the protein. The protein peptide fragment with high alpha-glucosidase inhibitory activity is obtained by virtually screening molecular docking, and the amino acid sequences of the protein peptide fragment are shown as SEQ ID No: 6, SEQ ID No: 9 and SEQ ID No: 24. The compound double-protein peptide sequence provided by the invention has an inhibiting effect on the activity of alpha-glucosidase, can be used for preventing and treating diabetes mellitus, and can be used for long-term health care or treatment of people with impaired glucose tolerance or diabetics as a functional food ingredient.

The invention relates to the field of preparation of materials for articular cartilage repair, in particular to collagen and chitosan cross-linked temperature-sensitive gel for articular cartilage repair and a preparation method of the collagen and chitosan cross-linked temperature-sensitive gel. The cartilage repair material prepared by the invention adopts the recombinant human collagen with a triple helix structure as a main raw material. The raw material is prepared by using a mammalian cell expression system, is close to protein folding and polymerization of natural protein, and has a spatial structure and modification necessary for active protein. The recombinant human collagen with the triple-helix structure is good in biocompatibility and free of rejection reaction and anaphylactic reaction, and the risk of disease transmission possibly caused by animal-derived collagen can be avoided. The injection type temperature-sensitive gel is liquid at low temperature and is converted into hydrogel at 37 DEG C, so that the injection type temperature-sensitive gel is suitable for being used as a transfer carrier for in-vivo injection treatment, secondary injury of an organism is effectively avoided, and the injection type temperature-sensitive gel has a wide clinical application prospect.

The invention belongs to the field of agriculture, and particularly relates to an agricultural functional active protein preparation rich in strontium, calcium and zinc and a preparation method thereof. The preparation method comprises the following steps: mixing traditional Chinese medicine raw materials, extracting, preparing a traditional Chinese medicine activation culture solution, and finally fermenting. According to the agricultural functional active protein preparation rich in strontium, calcium and zinc disclosed by the invention, trace elements such as strontium, calcium and zinc are all converted into organic states from chemical states after being biologically converted, so that the preparation has no toxic effect on soil and environment; after being biologically converted into an organic state, the leaf spray has high absorption efficiency on agricultural products, and the leaf spray absorption efficiency can reach 90% or above; leaf surface spraying can be carried out, use is convenient, the labor cost is low when the fertilizer is applied to each mu of land, and simultaneous supplement of multiple trace elements such as strontium, calcium and zinc is achieved; various pesticide residues can be rapidly and effectively degraded, and soil can be restored; the crop yield is increased; and the target organ quality is improved.

The invention provides a probiotic composition for improving immunity and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: fermenting selenium-enriched nostoc sphaeroides through selenium-enriched saccharomycetes to extract selenium-enriched nostoc sphaeroides polysaccharide and selenium-enriched nostoc sphaeroides protein peptide, mixing with traditional Chinese medicine polysaccharide obtained by extracting astragalus membranaceus, rhodiola rosea, codonopsis pilosula and pseudo-ginseng, and reacting with ferric salt to prepare a polysaccharide-Fe / selenium compound; solid residues are mixed and fermented, and active protein peptide is prepared from the solid residues and selenium-rich nostoc sphaeroides protein peptide; the probiotic composition capable of improving immunity is prepared by extracting a polysaccharide-Fe / selenium compound and a polysaccharide-Fe / selenium compound through an organic solvent, extracting a permeate through an organic solvent to obtain an active component, collecting a bacterial liquid, mixing and embedding the bacterial liquid in the polysaccharide-Fe / selenium compound and sodium alginate, and uniformly mixing the bacterial liquid with the active protein peptide, the active component, taurine and ginsenoside Rg3, so that the probiotic composition capable of improving immunity can play good anti-inflammatory, anti-oxidation, immunoregulation, anti-tumor, anti-virus and other roles, and has a good application prospect. Wide application prospects are realized.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

The utility model relates to the technical field of cow milk protein separation and purification, and provides a cow milk protein component synchronous separation system. Comprising a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line. Membrane filtration and a chromatographic technology are combined, and different active proteins are separated by using the molecular weight, the charged property, the hydrophobic interaction and the like of the proteins. Main active protein in milk can be separated, beta-casein, alpha-lactalbumin, immune globulin and lactoferrin can be sequentially separated and purified from raw milk, drying or sterile filling is carried out, different products are prepared while natural nutritional ingredients are reserved, and in addition, the product quality is improved. The separated milk can still be processed into sterilized milk or milk beverages.

A dual function protein is disclosed. The dual function protein may be prepared by linking a biologically active protein and an FGF mutant protein to an Fc region of an immunoglobulin. The dual function protein has improved pharmacological efficacy, in vivo duration and protein stability. The dual function protein exhibits improved pharmacological efficacy, in vivo duration and protein stability. A pharmaceutical composition containing the dual function protein as an active ingredient may be effectively used as a therapeutic agent for diabetes, obesity, dyslipidemia, metabolic syndrome, non-alcoholic fatty liver diseases, non-alcoholic steatohepatitis or cardiovascular diseases.

The invention discloses a river crab-snail breeding method based on space-time isolation and micro-flowing water circulation, and relates to the technical field of aquaculture, the key points of the technical scheme are that an isolation barrier is arranged in a river crab breeding pond, the river crab breeding pond is divided into a snail breeding area and a river crab main breeding area, and a water passing structure is arranged on the isolation barrier; a three-dimensional inhabiting base frame is arranged in the snail breeding area; generating directional water flow by utilizing oxygenation equipment to drive water in the main river crab breeding area to flow back after being filtered and purified by the snail breeding area; seed snails are put into the snail breeding area, and the snails grow and breed by feeding nutrient substances in the water body of the main river crab breeding area; collecting finished snails from the snail breeding area regularly and transferring the finished snails to the main river crab breeding area for feeding of the river crabs; in the river crab fattening period, the snail breeding area is opened, and the river crabs are allowed to freely eat the remaining snails. By means of the method, it can be guaranteed that snail populations rapidly breed to form a high-density biological filter bed, high-quality active protein bait can be provided for river crabs, and the win-win situation of water purification and feed cost reduction is achieved.

The earthworm active protein extraction device comprises a heating tank, an enzymolysis tank, a solid-liquid screener, an ultra-high-speed tubular centrifuge, a first buffer tank, a filter membrane assembly, a second buffer tank, a first concentration membrane assembly, a third buffer tank, a second concentration membrane assembly, a purification tank, a fourth buffer tank, a third concentration membrane assembly and a vacuum freeze dryer which are connected in sequence, according to the earthworm enzymolysis device disclosed by the utility model, the enzymolysis tank is used for carrying out enzymolysis on earthworms, so that compared with hydrolysis type enzymolysis, the treatment capacity of production process equipment can be reduced by 50%, the production time, the workload and the production cost are greatly reduced, water is saved, and the sewage treatment capacity is reduced; the tubular ceramic membrane is adopted, so that the concentrated solution is high in solid content and low in water content, and freeze drying is facilitated.

The present invention belongs to the field of biopharmaceuticals, and particularly relates to a multi-domain active protein for treating metabolic diseases. The multi-domain active protein has a structural formula as shown in Formula I: A-L a -F-L b -B. Compared with the prior art, the multi-domain active protein of the present invention has a long half-life and supports a once-weekly dosing frequency; the GLP-1R agonist activity of the multi-domain active protein is increased by more than 200 times at most; the multi-domain active protein has good stability in vivo and in vitro and low immunogenicity; since no unnatural amino acids need to be introduced and no chemical synthesis and cross-linking steps are involved, it can be prepared by a recombinant method, greatly simplifying the preparation process.

Variant or mutant prokaryotic cells are disclosed that express elevated or increased levels of a wide variety of active proteins. Also disclosed are methods of producing the elevated or increased levels of active encoded proteins.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

A logic-gated protein device in which proximity-gated protein trans-splicing governs formation of an active protein from two otherwise inactive fragments.

To provide a method for inducing an immune response against a tumor-associated antigen in a subject.SOLUTION: A method comprises administering to a subject a CMV vector encoding a tumor-associated antigen in an amount effective to induce a CD8+ T cell response against the tumor-associated antigen, where the CMV vector does not express an active UL128 protein, an active UL130 protein, an active UL146 protein, or an active UL147 protein or respective orthologs thereof, and where the tumor-associated antigen comprises a specific amino acid sequence of 15 amino acid residues (MHC-E or MHC-II supertope peptide).SELECTED DRAWING: None

The present invention provides targeting polyplexes comprised of (i) nucleic acids, in particular nucleic acids encoding pharmaceutically active peptides or proteins, and (ii) targeting conjugates comprising LPEI and PEG fragments that are connected by discrete linkages formed through defined, chemoselective reactions. Thus, the LPEI fragment is bonded in a linear end- to-end fashion to a single PEG fragment. The linear conjugates are further conjugated to a targeting fragment to enable selective interaction with a particular cell type. The polyplexes selectively deliver the nucleic acids to the targeted cells resulting in high expression and efficient protein translation as well as secretion of the encoded pharmaceutically active proteins.

The invention provides a hematopoietic anti-aging active component extracted from fish embryos as well as an extraction method and application of the hematopoietic anti-aging active component, and belongs to the technical field of active component extraction. The hematopoietic anti-aging active component is extracted from fish embryos which are developed from fish fertilized eggs to the earlier stage of incubation at the optimum temperature and stop development through rapid freezing, and is rich in exosomes, active protein, peptide, chondroitin sulfate, hyaluronic acid, small molecule metabolites, various active factors and the like, and all the nutritional active components are matched with one another, so that the effects of resisting aging and resisting aging are achieved. The cartilage development can be supported, the joint health is ensured, the hematopoietic effect is enhanced, and the skin aging is delayed. The orally disintegrating tablet containing the hematopoietic anti-aging active component can avoid inactivation of bioactive substances caused by high temperature, and the biological activity of the hematopoietic anti-aging active component is reserved to the maximum extent. The hematopoietic anti-aging active component and the orally disintegrating tablet are simple in preparation process and remarkable in hematopoietic anti-aging effect.

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The application provides a mass spectrometry detection device and method for active protein-small molecule interaction, which uses a mass spectrometry sensor to obtain tandem mass spectrometry data of non-covalent complexes of active protein incubated with small molecule compounds at different concentration points; performs non-linear curve fitting on the concentration sequence of the small molecule compounds based on the ion combination rate of the protein binding small molecules at each concentration point, to obtain an apparent dissociation constant of the active protein-small molecule interaction; determines a local conformation change parameter of the active protein induced by small molecule binding and a potential binding domain of the small molecule; and performs fusion evaluation on the binding strength and conformation influence degree of the protein-small molecule interaction based on the apparent dissociation constant, the local conformation change parameter and the potential binding domain, to output a stability grade of the protein-small molecule interaction. Based on the above scheme, non-denatured state mass spectrometry detection of protein-small molecule complexes in a liquid phase environment can be realized.

The invention discloses a colostrum-containing cheese and a preparation method thereof, wherein the colostrum-containing cheese comprises the following raw materials in parts by weight, based on 100 parts by total weight: 64-78 parts of cheese, 10-11.5 parts of trehalose, 1-1.4 parts of oligofructose, 0.5-0.7 parts of sodium citrate, 0.18-0.2 parts of locust bean gum, 0.16-0.19 parts of carrageenan, 0.4-0.45 parts of xanthan gum, 0.02-0.04 parts of nisin, and the rest are made up with colostrum. The present invention achieves the effect of improving the thermal stability of active proteins in cheese and colostrum through the mutual coordination of trehalose, oligofructose, sodium citrate, locust bean gum, carrageenan and xanthan gum, while meeting the requirement that microorganisms meet national standards, a higher active protein content can be maintained, and the taste is dense, mellow and delicious, and the advantages of stable quality during the shelf life.

The application provides high-protein long-shelf-life milk and a preparation method thereof. The preparation method comprises a step-by-step washing and filtering process, a continuous process without buffer storage, and a combination of ceramic membranes, centrifugal sterilization and pasteurization, prevents the loss of calcium ions in the concentration process, adjusts the lactose content to improve the taste, ensures high protein content, high active protein content, rich and sweet taste of the product, and improves the safety of the milk. Even in a more stringent 10-15 DEG C storage environment, the shelf life can reach 15 days.

The invention belongs to the field of nuclear medicine radiopharmaceuticals and relates to a double-target targeted polypeptide compound as well as a preparation method and application thereof. The structure of the compound or the pharmaceutically acceptable salt thereof comprises a TATE cyclic peptide structure, an FAPI-46 structure and a DOTA complexing group, wherein DOTA is used for complexing radionuclide; the DOTA complexing group is connected with two symmetrical phenylalanine through pentanic acid, so that the fat solubility of the DOTA complexing group is improved; the phenylalanine is respectively connected with the FAPI-46 and the TATE through the two hexanes, so that the steric hindrance of the FAPI-46 and the TATE is reduced. A radiopharmaceutical prepared from the double-target targeted polypeptide compound can be simultaneously combined with two targets, namely a somatostatin receptor and a fibroblast active protein, and has higher receptor binding affinity and higher tumor uptake through adjustment of lipid solubility and steric hindrance. Development of tumor-bearing animals shows that on SSTR2 / FAP < + > double-expression-bearing tumors, the double-target polypeptide drug is 4.8 times that of single-target TATE and 7 times that of single-target FAPI.

Disclosed herein is a stable apo (ligand-free)-active complex of a G protein coupled receptor (GPCR) and a G protein complex, wherein the G protein complex is a cognate heterotrimeric G protein comprising an alpha subunit (Gα), a beta subunit (Gβ) and a gamma subunit (Gγ). The apo-active GPCR-G protein complex can be isolated from a cell membrane in a purified form and maintains its stability in the purified form.

The invention discloses a method for producing feed granules containing antiviral proteins for protecting aquatic animals from viral infestation by passive immunization. The particles contain an effective amount of an active antiviral protein that is incorporated into the basal feed raw material together with a stabilizer, a gelling agent and a bioavailability enhancer. The antiviral protein is an IgY antibody contained in a hyper-immune egg powder obtained from an egg produced from an avian species exposed to an antigen associated with viral infection. In order to protect shrimps from white spot syndrome virus (WSSV), the antigens consist of proteins made of active portions of the VP28 and VP19 sequences of the WSSV. In one embodiment, the stabilizer is sodium alginate, the gelling agent is carboxymethyl cellulose, and the bioavailability enhancer is piperine. In one embodiment, the active protein and related supplements are incorporated throughout the feed pellet, and in another embodiment, it is sprayed on the surface of the feed pellet.

The invention relates to a preparation method of a specific inflammatory factor nucleic acid aptamer. The preparation method comprises the following steps: providing a high-purity active protein sample of inflammatory factors such as TNF-alpha, IL-16, C5a, MMP-8 or IL-3; designing an RNA library containing a random sequence, and covering a diversified secondary structure; the method comprises the following steps: eliminating a non-specific binding sequence by using a control protein (such as GM-CSF instead of IL-3) or a low-concentration target protein sample which is similar to a target protein in structure but irrelevant to a function through ultracentrifugation, magnetic bead sorting or ion-exchange chromatography technologies; incubating the library subjected to negative screening and high-purity target protein, and optimizing the binding specificity and affinity of the aptamer through multiple rounds of iterative screening by combining with a magnetic bead separation technology; high-throughput sequencing is adopted to analyze an enriched sequence, surface plasmon resonance (SPR) is utilized to determine affinity, interference of a conservative structural domain is eliminated through negative screening, functional sequences are accurately enriched in combination with positive screening, and the targeting of the aptamer to inflammatory factors is remarkably improved.

The invention provides an engineered exosome of overexpressed protein ILK as well as a preparation method and application of the engineered exosome. In particular to application of the engineered exosome of the overexpressed protein ILK in preparation of products for promoting osteogenic differentiation, the engineered exosome of the overexpressed protein ILK, a preparation method of the engineered exosome of the overexpressed protein ILK and a method for promoting osteogenic differentiation of human bone marrow mesenchymal stem cells. The protein molecule ILK is directly overexpressed on the basis of human-derived cells in a genetic engineering manner, so that the engineered exosome of endogenous enriched therapeutic active protein is generated, and the therapeutic activity for promoting bone regeneration is improved.

The present invention relates to an N-terminal extension sequence for enhancing the expression of a recombinant therapeutic peptide. The present invention also relates to a method for expressing a recombinant therapeutic peptide at a high level using the N-terminal extension sequence. The present invention also provides nucleic acids, vectors and recombinant host cells for efficiently producing biologically active proteins, such as liratide.

This invention discloses a fermented feed for improving the natural antibacterial and antioxidant properties of duck eggs. The fermented feed is prepared through a two-stage fermentation process using a fermentation substrate comprising the following components in parts by weight: 35-60 parts corn, 15-30 parts soybean meal, 5-15 parts wheat bran, 4-10 parts rice bran, 5-9 parts extruded flaxseed, 2-6 parts grape seed meal, 1-4 parts moringa flavonoid powder, 0.5-2.5 parts dicalcium phosphate, 0.5-3 parts limestone powder, 0.1-0.5 parts salt, and 0.1-0.8 parts compound premix. The two-stage fermentation involves first inoculating with Bacillus for aerobic fermentation, followed by inoculation with lactic acid bacteria and the addition of chitosan oligosaccharides for anaerobic fermentation. This invention significantly increases the content of active proteins such as lysozyme and ovotransferrin in duck egg white, enhancing its antibacterial properties and antioxidant capacity, and providing an efficient and economical technical path for the development of highly active duck egg white raw materials.