103 results about "Active protein" patented technology

The invention provides a probiotic composition for improving immunity and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: fermenting selenium-enriched nostoc sphaeroides through selenium-enriched saccharomycetes to extract selenium-enriched nostoc sphaeroides polysaccharide and selenium-enriched nostoc sphaeroides protein peptide, mixing with traditional Chinese medicine polysaccharide obtained by extracting astragalus membranaceus, rhodiola rosea, codonopsis pilosula and pseudo-ginseng, and reacting with ferric salt to prepare a polysaccharide-Fe / selenium compound; solid residues are mixed and fermented, and active protein peptide is prepared from the solid residues and selenium-rich nostoc sphaeroides protein peptide; the probiotic composition capable of improving immunity is prepared by extracting a polysaccharide-Fe / selenium compound and a polysaccharide-Fe / selenium compound through an organic solvent, extracting a permeate through an organic solvent to obtain an active component, collecting a bacterial liquid, mixing and embedding the bacterial liquid in the polysaccharide-Fe / selenium compound and sodium alginate, and uniformly mixing the bacterial liquid with the active protein peptide, the active component, taurine and ginsenoside Rg3, so that the probiotic composition capable of improving immunity can play good anti-inflammatory, anti-oxidation, immunoregulation, anti-tumor, anti-virus and other roles, and has a good application prospect. Wide application prospects are realized.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

The invention discloses a river crab-snail breeding method based on space-time isolation and micro-flowing water circulation, and relates to the technical field of aquaculture, the key points of the technical scheme are that an isolation barrier is arranged in a river crab breeding pond, the river crab breeding pond is divided into a snail breeding area and a river crab main breeding area, and a water passing structure is arranged on the isolation barrier; a three-dimensional inhabiting base frame is arranged in the snail breeding area; generating directional water flow by utilizing oxygenation equipment to drive water in the main river crab breeding area to flow back after being filtered and purified by the snail breeding area; seed snails are put into the snail breeding area, and the snails grow and breed by feeding nutrient substances in the water body of the main river crab breeding area; collecting finished snails from the snail breeding area regularly and transferring the finished snails to the main river crab breeding area for feeding of the river crabs; in the river crab fattening period, the snail breeding area is opened, and the river crabs are allowed to freely eat the remaining snails. By means of the method, it can be guaranteed that snail populations rapidly breed to form a high-density biological filter bed, high-quality active protein bait can be provided for river crabs, and the win-win situation of water purification and feed cost reduction is achieved.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

A logic-gated protein device in which proximity-gated protein trans-splicing governs formation of an active protein from two otherwise inactive fragments.

The present invention provides targeting polyplexes comprised of (i) nucleic acids, in particular nucleic acids encoding pharmaceutically active peptides or proteins, and (ii) targeting conjugates comprising LPEI and PEG fragments that are connected by discrete linkages formed through defined, chemoselective reactions. Thus, the LPEI fragment is bonded in a linear end- to-end fashion to a single PEG fragment. The linear conjugates are further conjugated to a targeting fragment to enable selective interaction with a particular cell type. The polyplexes selectively deliver the nucleic acids to the targeted cells resulting in high expression and efficient protein translation as well as secretion of the encoded pharmaceutically active proteins.

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The application provides a mass spectrometry detection device and method for active protein-small molecule interaction, which uses a mass spectrometry sensor to obtain tandem mass spectrometry data of non-covalent complexes of active protein incubated with small molecule compounds at different concentration points; performs non-linear curve fitting on the concentration sequence of the small molecule compounds based on the ion combination rate of the protein binding small molecules at each concentration point, to obtain an apparent dissociation constant of the active protein-small molecule interaction; determines a local conformation change parameter of the active protein induced by small molecule binding and a potential binding domain of the small molecule; and performs fusion evaluation on the binding strength and conformation influence degree of the protein-small molecule interaction based on the apparent dissociation constant, the local conformation change parameter and the potential binding domain, to output a stability grade of the protein-small molecule interaction. Based on the above scheme, non-denatured state mass spectrometry detection of protein-small molecule complexes in a liquid phase environment can be realized.

The application provides high-protein long-shelf-life milk and a preparation method thereof. The preparation method comprises a step-by-step washing and filtering process, a continuous process without buffer storage, and a combination of ceramic membranes, centrifugal sterilization and pasteurization, prevents the loss of calcium ions in the concentration process, adjusts the lactose content to improve the taste, ensures high protein content, high active protein content, rich and sweet taste of the product, and improves the safety of the milk. Even in a more stringent 10-15 DEG C storage environment, the shelf life can reach 15 days.

Disclosed herein is a stable apo (ligand-free)-active complex of a G protein coupled receptor (GPCR) and a G protein complex, wherein the G protein complex is a cognate heterotrimeric G protein comprising an alpha subunit (Gα), a beta subunit (Gβ) and a gamma subunit (Gγ). The apo-active GPCR-G protein complex can be isolated from a cell membrane in a purified form and maintains its stability in the purified form.

This invention discloses a fermented feed for improving the natural antibacterial and antioxidant properties of duck eggs. The fermented feed is prepared through a two-stage fermentation process using a fermentation substrate comprising the following components in parts by weight: 35-60 parts corn, 15-30 parts soybean meal, 5-15 parts wheat bran, 4-10 parts rice bran, 5-9 parts extruded flaxseed, 2-6 parts grape seed meal, 1-4 parts moringa flavonoid powder, 0.5-2.5 parts dicalcium phosphate, 0.5-3 parts limestone powder, 0.1-0.5 parts salt, and 0.1-0.8 parts compound premix. The two-stage fermentation involves first inoculating with Bacillus for aerobic fermentation, followed by inoculation with lactic acid bacteria and the addition of chitosan oligosaccharides for anaerobic fermentation. This invention significantly increases the content of active proteins such as lysozyme and ovotransferrin in duck egg white, enhancing its antibacterial properties and antioxidant capacity, and providing an efficient and economical technical path for the development of highly active duck egg white raw materials.

This invention relates to the field of biomaterials, specifically to the application of an acidic hydrolysate of a giant salamander mucus extract in the preparation of a root surface treatment agent. The root surface treatment agent comprises a product obtained by reducing lyophilized powder of salamander skin secretions with an acidic solution at a concentration of 0.2-0.3 mol / L and a pH of 1-5, wherein the mass ratio of the lyophilized salamander skin secretions to the acidic solution is (1-3):1; the resulting root surface treatment agent, after adjustment with an alkaline solution, has a pH of 6-6.5. This root surface treatment agent demonstrates significant potential in promoting natural tooth replantation and restoration, with effects including promoting early load stabilization through natural wet bonding, assisting in the construction of a regenerative microenvironment through active proteins, and promoting adhesion between periodontal ligament stem cells and the matrix by enhancing adhesion signal expression.

The invention relates to the technical field of biological medicine, in particular to an exosome medicine preparation for treating knee osteoarthritis and a preparation method thereof, and the exosome medicine preparation is characterized in that an exosome is derived from mesenchymal stem cells subjected to three-dimensional culture in a first hydrogel matrix containing sodium alginate and hyaluronic acid; flurbiprofen or a pharmaceutically acceptable salt or ester thereof; and a pharmaceutically acceptable second gel matrix. Sodium alginate and hyaluronic acid composite hydrogel serves as a three-dimensional culture carrier of mesenchymal stem cells, a joint microenvironment is simulated to induce stem cells to secrete high-activity exosomes rich in key functional nucleic acid and active protein, meanwhile, the exosomes are scientifically compounded with flurbiprofen, and synergistic mechanisms of anti-inflammatory analgesia and tissue repair are achieved; and a local slow-release delivery system is constructed through the second gel matrix, so that long-acting stable release of active ingredients is realized, the systemic side effect of the medicine is reduced, the bioavailability of the exosome is improved, and the problems of obvious side effect, limited repair effect and the like in the prior art are solved.

This invention relates to a botulinum toxin A1 precursor molecule, a nucleic acid molecule, an engineered bacterium, a method for preparing the same, and a method for preparing natural botulinum toxin A using the precursor molecule. The precursor molecule comprises a light chain and a heavy chain, and a linker region connecting the light and heavy chains. The amino acid sequence of the precursor molecule is shown in SEQ ID NO. 4. The light chain is the amino acid sequence from position 1 to position 444 of natural botulinum toxin A, as shown in SEQ ID NO. 5. The heavy chain sequence is the amino acid sequence from position 449 to position 1296 of natural botulinum toxin A, as shown in SEQ ID NO. 6. The amino acid sequence of the linker region is shown in SEQ ID NO. 11. This invention provides a safe process, and the resulting recombinant botulinum toxin not only has a sequence identical to the natural botulinum toxin active protein but also exhibits high activity, providing a low-cost, high-activity botulinum toxin product for clinical use.

The present invention provides a class of drug delivery systems for VHL-based protein degradation agents. Specifically, the invention provides a conjugate or a pharmaceutically acceptable salt thereof, and the conjugate is a conjugate using the drug delivery system as shown in formula (I). The conjugate provided by the invention can deliver the protein degradation agent to a target position in a targeted manner and release the active protein degradation agent.

The invention relates to the technical field of dairy product processing, in particular to Chinese yam milk containing active protein and a preparation method thereof, and the Chinese yam milk comprises the following raw material components: raw milk, Chinese yam powder, gellan gum, sucrose fatty acid ester, glyceryl monostearate, glyceryl distearate, amylase, glucoamylase, food essence and water. According to the method, the Chinese yam and the milk are combined, a sweetening agent is not additionally added, meanwhile, the beverage is endowed with a sweet feeling, meanwhile, the damage of heat treatment to active protein in cow milk in the processing process is overcome, and the Chinese yam milk which is good in flavor and stable in system and contains the active protein is prepared.

The disclosure is in the field of immunology and oncology, especially for treating, preventing, or inhibiting proliferative diseases, particularly cancer, infectious diseases and disorders associated with dysfunction of TNF cytokines. The disclosure relates to a novel SPD-TNFSF fusion protein including a TNF-superfamily (TNFSF) ligand, or receptor binding domain thereof, fused to a coiled-coil domain of surfactant protein-D (SPD). Also provided a trimeric or multimeric fusion protein including a plurality of SPD-TNFSF fusion proteins. The disclosure also provides an expression vector as mRNA, plasmid or virus including an isolated nucleotide sequence encoding the SPD-TNFSF fusion protein and a cell or a pharmaceutical composition including thereof.

The present disclosure provides the use of catalase or a variant thereof or a nucleic acid sequence or a carrier or a delivery system in the preparation of a pharmaceutical or cosmetic composition for ameliorating an inflammatory condition of photoaged skin.

The application discloses a method for collecting active proteins of water leeches, a detection method, an analysis method and application thereof, and relates to the technical field of biological medicines. The application provides the water leech seedlings treated by hunger and the living host treated by emptying, so that the water leech seedlings adsorb and suck the host, and then the active proteins are injected into the body of the host; the sample of the host after being sucked is collected, and then is made into a freeze-dried powder after being frozen and dried, so that the active proteins of the water leeches are obtained; the proteins are extracted from the freeze-dried powder, and then are subjected to enzymolysis treatment to generate peptide segments; the liquid chromatography-mass spectrometry technology is used to detect the peptide segments; and the mass spectrometry data are subjected to database searching analysis based on the protein sequence database of the water leeches and the host, so that the active proteins from the water leeches are identified and the relative content is determined. The application utilizes the characteristic that the active proteins are injected into the body of the host in the sucking process of the water leeches, and realizes efficient enrichment of the target proteins. In combination with the proteomics technology, high-throughput and high-sensitivity protein identification is realized.

The invention discloses a reporting system for intracellular evaluation of nsp3 protein PLpro cleavage activity as well as related protein and coding gene thereof. The reporting system comprises cleavage-inducing protein for intracellular evaluation of nsp3 protein PLpro cleavage activity and a GFP (Green Fluorescent Protein) detection reagent. The cleavage-induced protein can form a GFP (Green Fluorescent Protein) report protein-nsp2 / 3 cleavage site-PLpro active protein or a PLpro active protein-nsp3 / 4 cleavage site-GFP report protein. The reporting system not only indicates the activity of the PLpro, but also indicates various factors which can influence membrane localization and protein interaction in the nsp2 / 3 or nsp3 / 4 cutting process of the new coronavirus protein nsp3, so that the reporting system has the characteristics of low background, multiple targets and the like, and can be used for intracellular screening of a PLpro inhibitor and an inhibitor targeting the nsp2 / 3 or nsp3 / 4 process.

The present disclosure provides the use of catalase or a variant thereof or a nucleic acid sequence or a carrier or a delivery system in the preparation of a pharmaceutical composition or a cosmetic composition for scavenging reactive oxygen species (ROS), alleviating oxidative stress.

The application discloses a piezoelectric porous scaffold with high efficient osteogenesis and a preparation method thereof. The piezoelectric porous scaffold is obtained by mixing a biological component and a piezoelectric component through an adhesive, sintering and then performing polarization treatment. The piezoelectric porous scaffold can efficiently load and adsorb bioactive protein factors, realizes effective release of the protein, and has piezoelectric performance and osteogenesis activity. Under the multiple effects of the piezoelectric effect, the porous structure and the bioactivity of the protein of the scaffold, the piezoelectric porous scaffold can quickly start early response of bone marrow stromal cells in a synergistic manner, and then promote cell adhesion, proliferation and osteogenic differentiation. The piezoelectric porous scaffold has important significance for efficient and rapid repair of large bone defects, bone nonunion and other bone defects.

This invention generally relates to pharmaceutical formulations that can be used to treat acute coronary syndromes. More particularly, this invention relates to a novel pharmaceutical formulation comprising myeloid-derived growth factor (MYDGF) protein, which can be used to treat myocardial infarction in subjects of need. Compared to commonly used formulations, the pharmaceutical formulation of this invention provides significantly improved long-term stability. Furthermore, the pharmaceutical formulation of this invention allows for easy lyophilization and reconstitution without any significant loss of therapeutically active protein. This invention also relates to a formulation for use in a method of treating myocardial infarction in subjects of need. The method includes intravenous administration of MYDGF protein to the subject in addition to standard care.

The present application discloses PLPC-DB, an ultrapurified phospholipoproteomic composition derived from the supernatant of peripheral blood mononuclear cells (PBMCs). It comprises essential phospholipids, bioactive proteins, regulatory peptides, and intercellular signaling factors. The composition exceeds 99% purity through a multi-stage purification process combining high-speed centrifugation and selective ultrafiltration (1-50 kDa), followed by lyophilization. This process ensures structural stability for ≥24 months and inter-batch variability <2%. PLPC-DB supports reproducibility and molecular integrity in research and diagnostic settings. Key components include phosphatidylcholine and phosphatidylserine for membrane stabilization and intracellular signaling; structural and regulatory lipids for membrane biogenesis and immune-relevant components involved in antigen presentation, cytokine modulation, and membrane-associated signaling. These may include structurally defined molecules compatible with immunological evaluation in experimental and non-therapeutic settings. The composition is formulated for bioaccessible delivery via sublingual, endonasal, transdermal, and injectable routes, and is intended exclusively for non-therapeutic use in experimental and translational models.

Disclosed are methods of producing conditionally active proteins that target senescent cells and are conditionally active in the extracellular environment of senescent cells. The methods include methods of using an evolved protein library and testing using physiological concentrations of bodily fluid components. Also disclosed are conditionally active proteins, antibodies, and antibody fragments for killing or removing senescent cells, conjugates and pharmaceutical compositions using these conditionally active proteins, and methods of treating age-related diseases, conditions, or disorders using the pharmaceutical compositions. Conditionally active proteins can be further evolved, conjugated to other molecules, masked, and reduced in activity by linking cleavable moieties.

The invention relates to the technical field of egg white protein extraction, and discloses stem cell egg powder derived from fertilized eggs and a preparation method and application of the stem cell egg powder. According to the method, a specific low-temperature graded salting-out path is constructed, impurities such as ovomucin are selectively removed through the first-stage sodium sulfate precipitation, the purity of the extract is improved, the purity of the extract is improved, and the purity of the extract is improved. And a clean solution background is created for the second-stage sodium chloride precipitation, so that the unification of high-activity retention and high-purity separation of the target active protein is realized under low-temperature operation in the whole process.

The invention belongs to the field of biological medicine, and discloses a mesenchymal stem cell-derived exosome and active factor secretion composition and a directional preparation method thereof. According to the method, umbilical cord tissues of healthy mammals are used as raw materials, primary stem cells are separated through enzymolysis, and amplification and passage are conducted to 3-5 generations through an alpha-MEM culture medium; a serum-free induction culture medium containing multiple functional components is adopted for directional induction, a conditioned culture medium is collected, gradient centrifugation and ultrafiltration concentration are performed, and the protein concentration is calibrated to be 2 mg / mL, so that a finished product is obtained. The product can synergistically realize moisturizing, anti-wrinkle and tightening effects. Specifically, the exosome and active factor secreted composition is a composite system which is obtained by separating and concentrating mesenchymal stem cells from a conditioned medium of the mesenchymal stem cells after the mesenchymal stem cells are directionally induced by the method and is mainly composed of the exosome, growth factors self-secreted by the stem cells, cell factors and macromolecular active protein.

The invention belongs to the technical field of biological medicine and nano delivery, and particularly relates to a protein crown delivery system and a preparation method and application thereof. According to the protein crown delivery system provided by the invention, the specific bifunctional polypeptide is utilized to modify the surfaces of the gold nanoparticles, and various active proteins can be efficiently and stably combined to form the protein crown, so that the active proteins can be delivered in cells. Specifically, rich side chain groups of bifunctional polypeptide molecules are utilized to solve the problem of few binding sites between a protein crown delivery system and protein molecules, so that the protein crown delivery system and the protein molecules are combined and assembled through non-covalent interaction to form a uniform and stable protein crown structure, and the binding efficiency of a carrier and protein is improved; therefore, a universal, efficient and safe protein nano intracellular delivery carrier is constructed. The protein crown delivery system provided by the invention can be coupled with various types of proteins, and keeps the biological activity and function of the proteins, so that intracellular delivery can be performed on the proteins with different activities.

A protein carrier-based hair follicle transdermal delivery system for an active ingredient, which is of a composite structure composed of a small molecule active ingredient, a bioactive protein and a polymeric shell layer, with a size of 40-150 nm and a thickness of the polymeric shell layer of 10-70 nm. The protein carrier-based hair follicle transdermal delivery system for an active ingredient exhibits a positive Zeta potential. The small molecule active ingredient is loaded in the bioactive protein, and the polymeric shell layer coats the bioactive protein to form a protein nanogel. The small molecule active ingredient and the bioactive protein have beneficial active effects on hair follicles. By means of applying the hair follicle transdermal delivery system to the surface of hair follicles or skin, the functional small molecule is transdermally delivered to the interior of the hair follicles and surrounding tissues thereof along with the nanogel, and then released in a controlled manner, thereby enabling the functional small molecule to exert the effects effectively.