261 results about "Human bone" patented technology

The invention provides a microfluidic chip, system and method for osteochondral organs, and relates to the technical field of organoid microfluidic chips, the chip comprises a bone microvascular endothelial cell channel, a bone compartment, a cartilage compartment and a synovial fluid channel which are arranged in parallel and in sequence, and permeation areas between the channel and the compartments and between the compartments. According to the scheme, the complex structure and function of a human osteochondral system are effectively simulated, the physiological and pathological processes of human bone joints can be simulated more truly in an in-vitro environment, and a method for establishing a highly-simulated femoral head necrosis model and osteoarthritis model based on the chip is provided. The problems that a traditional model is insufficient in physiological environment simulation, lack of control over dynamic culture conditions, tedious and time-consuming in the construction process, difficult to support high-throughput experiments and the like are solved.

Various embodiments of implant systems and related apparatus, and methods of operating the same are described herein. In various embodiments, an intermedullary implant for interfacing with a bone structure includes a web structure, including a space truss, configured to interface with human bone tissue. The space truss includes two or more planar truss units having a plurality of struts joined at nodes. Implants are optimized for the expected stress applied at the bone structure site.

Long COVID or Post Acute Sequelae of COVID-19 (PASC), is a prolonged, debilitating syndrome that follows acute SARS-CoV-2 infection in >10% of cases. Human bone marrow mesenchymal stem cell derived extracellular vesicles (hBM-MSC EVs) can present a new therapeutic option.

The invention discloses a soluble expression and purification method of recombinant human bone morphogenetic protein in a prokaryotic system. A gene sequence of a recombinant human bone morphogenetic protein (rhBMP) is spliced at the 3'terminal of a His-Linker-GB1 gene sequence, inserted into a prokaryotic expression system expression vector and transferred into a prokaryotic competent cell to be highly expressed in the cell in a soluble form, and finally, the expressed His-Linker-GB1-BMP fusion protein does not need to be subjected to inclusion body renaturation, so that the recombinant human bone morphogenetic protein (rhBMP) is obtained. The high-purity fusion protein can be obtained through one-step metal chelating chromatography (adsorption-elution mode), and the GB1 tag has small molecular weight and hardly affects the spatial structure and biological activity of rhBMP, so that the situation that an inclusion body is formed by expression of rhBMP in a traditional escherichia coli system and expression is performed in a structure-free and activity-free inclusion body form is avoided, and the expression efficiency is greatly improved. The method is simple and convenient to operate, is suitable for large-scale amplification, and has the characteristics of short period, high purity, stable process and the like.

The invention relates to bone paste for repairing bone wounds and a preparation method of the bone paste, and belongs to the technical field of biomedical material manufacturing. The bone paste is prepared from nanoscale calcium-phosphorus biphase ceramic, biomimetic mineralized collagen fiber, biodegradable polymer, modified hyaluronic acid, beta-cyclodextrin coated recombinant human bone morphogenetic protein-7, an angiogenesis accelerant and an immunoregulation additive according to a certain proportion. The nano-scale calcium-phosphorus dual-phase ceramic is hydroxyapatite and beta-tricalcium phosphate dual-phase ceramic; the biomimetic mineralized collagenous fiber is prepared by mixing type I collagen freeze-dried fiber with hydroxypropyl chitosan and performing mineralization; the modified hyaluronic acid is hyaluronic acid-sulfydryl and hyaluronic acid-hydrazide; the angiogenesis accelerant is a polylactic acid sustained release microsphere loaded with tanshinone and hydroxyl polyethylene glycol biotin; the immunoregulation additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction capacity, promote vascularization generation and reduce the immunogenicity risk.

The invention provides a silicon nitride gradient structure biological ceramic and a preparation method thereof, and belongs to the field of biological ceramics. The preparation method of the silicon nitride gradient structure biological ceramic comprises the following steps: preparing outer bearing layer slurry, performing slip casting, preparing an inner contact layer filler, performing filling molding, and performing post-treatment. According to the silicon nitride gradient structure biological ceramic and the preparation method thereof, the defect of low toughness of the biological ceramic is effectively overcome, the toughness of the biological ceramic is improved, the bearing performance of the biological ceramic in a human body is ensured, the mechanical stability is improved, the bonding stability of different functional layers of the biological ceramic is improved, and the defect of stress concentration is eliminated; the overall stability of the biological ceramic is improved, and the problems of layering and interface cracking among different functional layers are avoided; the bonding performance of the biological ceramic and human bone tissues is further improved, so that good growth of human bone tissues in contact with the biological ceramic is ensured.

The invention relates to a recombinant collagen bionic artificial bone and a preparation method and application thereof.The recombinant collagen is subjected to cross-linking modification, the molecular weight and viscosity of the recombinant collagen are increased, a fiber network structure is formed, then the calcium-phosphorus ratio of hydroxyapatite is simulated through soluble salts of calcium chloride and sodium dihydrogen phosphate, and the recombinant collagen bionic artificial bone is obtained. The biomimetic artificial bone can replace insoluble hydroxyapatite to mineralize the recombinant collagen, so that the utilization rate of the collagen is improved, and the obtained biomimetic artificial bone can exert a better osteogenesis effect. The obtained bionic artificial bone is high in biological safety, has high bionic characteristics, good biocompatibility and mechanical properties matched with human bones, can induce bone marrow mesenchymal stem cell osteogenic differentiation, and has great potential in the field of bone defect repair.

The invention belongs to the technical field of biological genetic engineering, and relates to a high-risk myelodysplastic syndrome screening gene and a diagnostic kit thereof. The invention provides an application of detecting YTHDF2, METTL3 and NAT10 gene expression of bone marrow cells of high-risk MDS patients as a specific molecular marker for screening the high-risk myelodysplastic syndrome, and by detecting mRNA expression levels of the YTHDF2, METTL3 and NAT10 genes, the screening of the patients in the high-risk myelodysplastic syndrome is facilitated. Meanwhile, treatment and prognosis evaluation of patients with high-risk myelodysplastic syndromes can be better guided. Only the bone marrow fluid of the patient needs to be extracted and collected during bone marrow cytology examination of the patient, the patient compliance is good, the bone marrow fluid is selected from human bone marrow fluid mononuclear cells, the detection result is more accurate, and the method is suitable for clinical large-scale application.

The invention provides a gradient density metal implant additive manufacturing method, and relates to the technical field of selective laser melting additive manufacturing, according to the method, continuous gradient density distribution of a metal implant from an outer layer to an inner layer is achieved through multi-mode sensor fusion and a self-adaptive control algorithm, biomechanical characteristics of a skeleton are accurately simulated, and the manufacturing precision of the metal implant is improved. The step of designing a porous structure is omitted; cortical bone (high density) and cancellous bone (porous) structures of human bones can be accurately simulated, and the biomechanical suitability of the implant is improved.

The invention discloses an antibacterial magnetic compatible medical beta-type Zr-Nb-Cu alloy and a preparation method thereof. The alloy is prepared from, by mass, 18%-24% of Nb, 4%-10% of Cu and the balance Zr and inevitable impurity elements. The preparation method of the alloy comprises the following steps: 1, weighing sponge zirconium, copper and niobium particles as raw materials; 2, putting the raw materials into a water-cooled copper crucible, vacuumizing, introducing argon, and smelting in a non-self-consumption vacuum arc furnace; according to the beta-type Zr-Nb-Cu alloy, alloying elements Nb and Cu are selected, the strength, hardness, abrasion resistance and corrosion resistance of the alloy are improved while the elasticity modulus of the alloy is reduced, the lasting and efficient antibacterial performance of the alloy is guaranteed, the influence of postoperative bacterial infection and MRI detection artifacts is remarkably reduced, and therefore the beta-type Zr-Nb-Cu alloy has the lower elasticity modulus and magnetic susceptibility, and the service life of the alloy is prolonged. And the method is more suitable for human skeleton and nuclear magnetic resonance (MRI) detection.

The invention discloses a method for constructing an organoid. The method comprises the following steps: (1) carrying out hanging drop culture on testicular supporting cells and primordial germ cell-like cells in an organoid culture solution according to a quantity ratio of 1: (2-6) to obtain cell aggregates; (2) culturing the cell aggregate in a gas-liquid phase culture system to obtain an organoid; wherein the content of double positive cells in the primordial germ cell-like cells is greater than 40%, the gas-liquid phase culture system comprises a solid culture medium and an organoid culture solution, and the solid culture medium is formed by the organoid culture solution and agarose gel; wherein the organoid culture solution is prepared from human bone morphogenetic protein-4, a basic fibroblast growth factor, a glial cell-derived neurotrophic factor and retinoic acid. The organoid obtained by the method has a high germ cell population proportion.

The present invention relates to a bone trauma repair bone paste and a preparation method thereof, belonging to the technical field of biomedical material manufacturing. The bone paste of the present invention is prepared by mixing nano calcium phosphate biphasic ceramics, biomimetic mineralized collagen fibers, biodegradable polymers, modified hyaluronic acid, β-cyclodextrin-coated recombinant human bone morphogenetic protein-7, angiogenesis promoters and immunomodulatory additives in a certain proportion; the nano calcium phosphate biphasic ceramics are hydroxyapatite and β-tricalcium phosphate biphasic ceramics; the biomimetic mineralized collagen fibers are prepared by mineralizing freeze-dried fibers of type I collagen admixed with hydroxypropyl chitosan; the modified hyaluronic acid is hyaluronic acid-thiol and hyaluronic acid-hydrazide; the angiogenesis promoter is a polylactic acid sustained-release microsphere loaded with tanshinone and hydroxy polyethylene glycol biotin; the immunomodulatory additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction ability, promote angiogenesis and reduce the risk of immunogenicity.

In various embodiments, an implant for interfacing with a bone structure includes a web structure including a space truss. The space truss may have a plurality of struts joined at nodes and the web structure is configured to interface with human bone tissue. At least some struts may form an irregular pattern in the web structure. The space truss may include two or more planar truss units having a plurality of struts joined at nodes.

The invention relates to the technical field of biomedical materials, in particular to a preparation process of novel composite bone cement with biological activity, which comprises the following steps: calcining calcium phosphate in a calcining furnace at 800-1000 DEG C for 3 hours, cooling to 20 DEG C, grinding the calcium phosphate by using a grinding machine until the diameter is less than 100 mu m, and drying to obtain the novel composite bone cement with biological activity. Calcium phosphate micro powder is obtained; by fusing the calcium phosphate, the nano-hydroxyapatite, the bioglass and the collagen, the material has a mechanical support function of an inorganic phase and biological activity of an organic phase, the nano-hydroxyapatite is extremely similar to human bone minerals and can promote osteoblast attachment and growth, and the collagen constructs a bionic microenvironment and accelerates new bone formation, so that the material can be used for treating osteoporosis. The bone repair efficiency is obviously improved.

The invention provides a model training method and device for extracting human skeleton features. The method comprises the following steps: acquiring original computed tomography (CT) data of different target objects belonging to the same group for human bones of a target part; reconstructing a three-dimensional model of each target object for the target part based on the original CT data to obtain a model file corresponding to each three-dimensional model; extracting point cloud data corresponding to the three-dimensional model from each model file, and generating label data corresponding to each voxel in the three-dimensional model in batches; and taking the point cloud data of all the three-dimensional models and the label data of each voxel in all the three-dimensional models as a training set, training a deep learning three-dimensional model introduced with dynamic graph convolution until a preset stop condition is met, and obtaining a human skeleton mark point recognition model. According to the technical scheme provided by the invention, the accuracy of identifying the human skeleton mark points by the human skeleton mark point identification model can be improved.

The invention provides a sleep detection method and device, electronic equipment and a storage medium, and belongs to the technical field of artificial intelligence, and the method comprises the steps: inputting an image frame in an obtained video stream into a target detection model, so as to output a human body frame and a face frame of a target object in each frame of image; extracting a human body image from the human body frame, inputting the human body image into a human body skeleton point detection model to obtain a skeleton point information sequence, and inputting the skeleton point information sequence into a human body resting state detection model to obtain human body state parameters; extracting a face image from the face frame, and respectively inputting the face image into a face key point detection model and a health monitoring model to obtain corresponding eye activity parameters and physiological activity parameters; and determining whether the target object is in a sleep state or not according to at least one of the human body state parameters, the eye activity parameters and the physiological activity parameters. According to the method, the problems of robustness and accuracy in user sleep detection are effectively solved.

A method for detecting the number of juggling times in football training and a computer-readable storage medium, the method comprising: step 1, obtaining a juggling training video; step 2, intercepting the juggling training video into multiple frames of images, and sequentially inputting them into a trained football juggling key point detection network to obtain human skeleton point information and football point information in each frame of the image; step 3, based on the human skeleton point information and football point information in the image, judging whether the juggling condition is met according to the correlation between the football point and the human skeleton point during the juggling process, and if the juggling condition is met, it is recorded as one juggling; step 4, sequentially traversing the images to obtain the number of juggling times in football training. The present invention collects the required football training video through a user device with a camera function, and can accurately detect the number of football training juggling times of a football trainer through subsequent processing, which changes the existing method of observing and counting by a coach or a fellow trainee in the football training process.

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

The invention provides a bionic 3D printing degradable magnesium alloy interbody fusion cage and a preparation method thereof, the bionic 3D printing degradable magnesium alloy interbody fusion cage comprises a porous frame and a through hole net rack, the porous frame is constructed by supporting rods with the diameter of about 0.4 mm, the porous frame is of a compact rhombic hole structure, the aperture is about 200-400 microns, the pore coverage rate is about 50%-80%, and a horseshoe-shaped columnar space is defined; the through-hole net rack is formed by building supporting rods with the diameter of 0.2-0.1 mm, the hole diameter is about 300-800 microns, the hole coverage rate is about 90%, a dome structure is formed in the center, and the through-hole net rack is arranged in the middle of the porous frame. The magnesium-strontium alloy is adopted as a base material of the interbody fusion cage, the interbody fusion cage is integrally formed through the 3D printing technology, and the degradable characteristic and good biocompatibility of the magnesium alloy are fully utilized. The elasticity modulus of the magnesium alloy is closer to that of the human bone tissue, physiological loads can be better shared and transmitted, the stress shielding effect is reduced, and growth and fusion of the bone tissue are promoted.

The invention discloses a bionic Voronoi-nickel titanium-hydrogel heterostructure implant and a preparation method thereof, and relates to the technical field of bone implantation, the construction of a Voronoi porous structure and the preparation of a bionic Voronoi-nickel titanium skeleton are completed through an innovative design method by referring to the structural concept of human bone trabecula, and then the bionic Voronoi-nickel titanium skeleton is used as a substrate to prepare the bionic Voronoi-nickel titanium hydrogel heterostructure implant. And preparing the bionic Voronoi-nickel titanium-hydrogel heterostructure implant by adopting a drying gel forming method. The implant integrates the mechanical advantages of high strength and low modulus of the nickel-titanium alloy and the structural characteristics of high connectivity and high specific surface area of the bionic Voronoi porous structure, the defect that hydrogel is weak in bearing is effectively overcome, meanwhile, the hydrogel has the buffering and energy absorbing effects, multifunctional time sequence delivery and release of antibiosis, anti-inflammation and bone regeneration promotion can be achieved, and the requirements of all stages of bone repair are met.

The present invention belongs to the field of biological genetic engineering technology and relates to high-risk myelodysplastic syndrome screening genes and diagnostic kits. The present invention provides an application of detecting the expression of YTHDF2, METTL3, and NAT10 genes in bone marrow cells of high-risk MDS patients as a specific molecular marker for screening high-risk myelodysplastic syndrome. By detecting the expression levels of YTHDF2, METTL3, and NAT10 genes mRNA, it helps to screen patients with high-risk myelodysplastic syndrome and better guide the treatment and prognosis evaluation of patients with high-risk myelodysplastic syndrome. The present invention only needs to extract the patient's bone marrow fluid and collect it together when the patient undergoes bone marrow cytology examination. The patient compliance is good, and the material is taken from human bone marrow fluid mononuclear cells. The test results are more accurate and suitable for large-scale clinical application.

The invention relates to the technical field of biology, in particular to application of a GGPS1 activator, a pharmaceutical composition, a kit and a method. The invention relates to bone metabolism regulation and bone regeneration. It is found that improvement of GGPS1 expression / activity can activate TGF-beta / Smad2 / 3 by down-regulating LTBP1, so that human bone marrow mesenchymal stem cells are converted from adipogenesis to osteogenesis, and bone formation and bone defect repair are promoted. The invention provides a drug containing a GGPS1 activator and a controlled release composition for implantation, which are used for osteoporosis, bone mass reduction, delayed fracture healing and the like. The invention also provides an in-vitro screening and evaluation method and a detection kit, and the indexes such as p-Smad2 / 3, alizarin red, oil red O and the like are used for judgment. The invention realizes upstream heavy balance and has the advantages of bone promotion and fat suppression.

The invention relates to the technical field of medical instruments, in particular to a device and method for testing the fixing strength of a wired anchor, and the device comprises a lower tool which comprises a sliding seat and two clamping assemblies, the two clamping assemblies are arranged on the sliding seat in a sliding manner, and the clamping assemblies move oppositely to clamp a test block; the test block is a polyurethane block, a through hole is formed in the test block, four groups of tendon ligament simulants vertically penetrate through the through hole, anchor nails with lines are fixed in the through hole, and the anchor nails with lines are screwed into or knocked into the through hole and extrude and fix the tendon ligament simulants; the upper tool comprises a box body and a balance assembly, the balance assembly is arranged in the box body, the lower end of the balance assembly is provided with a chuck for clamping a tendon ligament simulant, the lower tool clamps a test block for simulating a human skeleton, and the upper tool clamps the tendon ligament simulant located at the upper end of an anchor nail with a line in the test block; the problems that an independent anchor is difficult to clamp due to the appearance and clamping is prone to deformation due to the material strength of the anchor with the line are solved.

A device (100) for mobilizing the human bone-tendon system, the device comprising: - a knee protector (2) arranged to cover a knee (3) of a human user to hold said knee in said knee protector and to exert a mobilization force on the knee covered by said knee protector, said mobilization force exerting pressure on said knee and directing towards a load bearing surface (5) arranged to support the pelvis of the user, and - means (6) for increasing the mobilization force.

The invention discloses a bone repair material as well as a preparation method and application thereof. The bone repair material is prepared from recombinant human collagen, recombinant human bone morphogenetic protein 2 and hydroxyapatite, wherein the ratio of the inorganic matter to the organic matter in the bone repair material is (64-66): (36-34). The bone repair material disclosed by the invention takes the recombinant human collagen as a core raw material, so that the components and the structure of the prepared material are ensured to be highly consistent with those of natural bone tissues; secondly, by adding the recombinant human bone morphogenetic protein 2, the osteogenic activity of the material is further enhanced. The finally obtained recombinant human collagen active bone repair material completely retains the triple helix structure of the recombinant human collagen, so that the recombinant human collagen active bone repair material has complete biological activity, and also has the outstanding advantages of good osteogenic activity, no disease and virus transmission risk and no immunogenicity.

The utility model belongs to the technical field of medical instruments, and relates to a joint implant for the orthopedics department. The joint implant is used for joint bone cartilage injury and defect repair and comprises a joint implant base body with a porous titanium structure and a top solid self-lubricating film. The joint implant base includes a top region, an upper region, and a lower region. The top area is matched with the outer contour face of the human body bone and is of a dense mirror face structure, the surface of the top area is plated with a solid self-lubricating film, abrasion to the opposite-side cartilage can be reduced to a large extent, and degeneration of the opposite-side cartilage is reduced. The side faces of the top area and the upper area of the joint implant base body make contact with subchondral bones and cancellous bones of the human body, and the lower area of the joint implant base body makes contact with the cancellous bones of the human body. The aperture and porosity of the joint implant base body extend from the upper area to the lower area, are increased in a gradient manner, are matched with the mechanical properties of human bones, and are beneficial to initial stability, early-stage rapid bone ingrowth and long-term stability.

Various embodiments of implant systems and related apparatus, and methods of operating the same are described herein. In various embodiments, an implant for interfacing with a bone structure includes a web structure, including a space truss, configured to interface with human bone tissue. The space truss includes two or more planar truss units having a plurality of struts joined at nodes. Implants may include biodegradable polymer particles contained within biocompatible fibers. The biodegradable polymer particles may include bone growth promoting agents that are released as the particles degrade over time.

The present invention relates to an automatic "scissors"-type bone groove decompression head device for spinal surgery, which includes a head connecting seat. A stepped cavity is arranged inside the head connecting seat, and a telescopic guiding device is arranged inside the stepped cavity. The telescopic guiding device includes a telescopic column. An adjustment space is arranged between the stepped cavity and the telescopic column. An upper telescopic spring is arranged at the upper end of the adjustment space, and a lower telescopic spring is arranged at the lower end of the adjustment space. The upper end of the telescopic column is connected with a bone cutting power device, and an electric knife and a suction device are arranged inside the telescopic column. Thus, through the cooperation between the clip and the working channel, a clip structure can be formed, which is convenient for clamping the spinous process for positioning. By the vertex of the clip, the head is rotated to judge whether the head is perpendicular to the human bone, which is convenient for improving the surgical precision. The telescopic column automatically adapts to the height change of the lower surface of the bone, and the bone cutting power device and the electric knife hook form a "scissors-like" structure, which is beneficial to safely realizing bone groove decompression.

The invention discloses sclera shell slurry suitable for ceramic photocuring 3D printing and a preparation method of the sclera shell slurry, and relates to the technical field of biomedical materials and additive manufacturing, and the sclera shell slurry comprises 2.0%-5.0% wt of methacrylated gelatin, 70%-90% wt of nano-hydroxyapatite, 10%-25% wt of a photosensitive resin solution, 1%-3% wt of a composite photoinitiator and 1%-5% wt of a composite dispersion stabilizer. The high-solid-content slurry and the optimized sintering process disclosed by the invention ensure that the final ceramic product has enough mechanical strength similar to that of human bones, scar contracture can be effectively resisted, and a long-term stable physical barrier is provided. And the process is stable, the forming precision is high, high fluidity and low viscosity of the slurry are guaranteed through unique powder grading and a dispersion stable system, the photocuring printing process is smooth, the forming structure resolution ratio is high, and defects are few. The whole set of process has clear parameters and good reproducibility.

The invention discloses polymethyl methacrylate bone cement as well as a preparation method and application thereof, and belongs to the technical field of bone cement. The elasticity modulus of the polymethyl methacrylate bone cement does not exceed 2GPa, and the polymerization peak temperature of the polymethyl methacrylate bone cement does not exceed 90 DEG C. The polymethyl methacrylate bone cement has relatively low elastic modulus and polymerization temperature, is beneficial to improving the biocompatibility and matching degree of the bone cement and human bone tissues, and can be used for reducing thermal damage to surrounding tissues or nerves caused by polymerization heat release; or is used for improving elastic matching with human bone tissues so as to reduce the risk of stress-related complications. The preparation method of the polymethyl methacrylate bone cement is simple and easy to operate, and can be used for industrial production.