8 results about "Bone matrix" patented technology

The application relates to the technical field of biological medicine, and discloses a preparation method of a bone melon extract powder injection and a pharmaceutical composition, which comprises the following steps: bone raw material pretreatment, bone polypeptide extraction, melon seed raw material pretreatment, melon seed polypeptide extraction, stabilizer composition preparation and freeze-dried preparation; through deep defatting and decalcification treatment on the bone raw material, and in combination with specific condition enzymolysis and two-step ultrafiltration purification processes, non-target impurities in the bone matrix can be removed, and the molecular weight distribution range of the bone polypeptide can be controlled, so that the uniformity and batch consistency of the active ingredient of the bone polypeptide are improved. Meanwhile, the compound protease enzymolysis strategy used in the bone polypeptide extraction can synergistically act on the bone protein, improves the polypeptide yield, and lays a foundation for the stable control of the active ingredient proportion in the final pharmaceutical composition.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 μm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

This application discloses a composition, conditioning agent, and application for protecting bone health. The active ingredients of the composition for protecting bone health include purslane powder, cucumber powder, celery powder, and Solomon's seal powder. This application scientifically combines the four active ingredients to fully leverage their synergistic effects, effectively activating the Runx2 gene and its expression, promoting the production of bone matrix proteins, and significantly intervening in and improving bone discomfort caused by bone matrix protein deficiency. Furthermore, the composition uses naturally derived active ingredients, exhibiting high safety, excellent biocompatibility, and no obvious side effects. The good compatibility between the active ingredients allows for preparation into various dosage forms according to actual needs, making application convenient and with high compliance. Thus, this provides an effective functional active composition for protecting bone health.

The application discloses a gradient biomimetic PEEK-HA-Col I composite implant and a preparation method and application thereof, relates to the technical field of medical biomaterials, and has the following scheme: a composite implant composed of a load-bearing inner layer, a bioactive intermediate layer and a biomimetic bone matrix layer is constructed; the load-bearing inner layer contains a PEEK matrix, the intermediate layer contains PDA modified interfaces and HA induced and deposited by the interfaces, and the outer layer contains cross-linked modified collagen type I, and a bioactive gradient distribution is formed along the thickness direction; the preparation method and the application of the composite implant in bone repair are simultaneously provided. The application improves the interface bioactivity and the bone integration capability on the basis of maintaining the PEEK load-bearing support, improves the interface structure stability and the bioactive spatial distribution, improves the surface hydrophilicity and the interface microenvironment, is favorable for promoting early osteogenesis and bone defect repair, improves bone fusion and application adaptability in a load-bearing bone implantation scene, and simultaneously considers the synergistic matching of the load-bearing demand and the biological demand of the bone tissue interface.

This invention discloses a bone repair scaffold material, its preparation method, and its application. The preparation method includes the following steps: preparation of a mineralized collagen core spinning solution; adding mangiferin to a PLGA electrospinning solution and stirring to obtain a shell spinning solution; and using the core spinning solution and the shell spinning solution, obtaining the bone repair scaffold material through coaxial electrospinning. In this invention, PLGA and mangiferin together form the shell of the scaffold structure, and the sustained release of mangiferin provides a guarantee for the anti-inflammatory and angiogenic effects of the scaffold material. Simultaneously, it simulates the fibrous structure of the bone matrix, achieving slow degradation of the core and achieving the goal of long-term promotion of bone repair.

This invention relates to the field of biomedical materials technology, specifically to a decellularized matrix microparticle-based ink for extrusion 3D printing and its preparation method. The key technical points are: dispersing decellularized matrix microparticles (dECM-MPs) in a metal ion solution for ion adsorption; the dECM-MPs include cartilage matrix microparticles (DCM-MPs) or bone matrix microparticles (DBM-MPs); then, without damaging the structure and composition of the dECM-MPs, forming a hydrogel microlayer in situ on the surface of the dECM-MPs through interfacial ion diffusion crosslinking; centrifuging the dECM-MPs encapsulated in the hydrogel microlayer to obtain an ink suitable for extrusion 3D printing; and using this 3D printing ink to print and then fixing it with ultraviolet light to obtain a 3D printed implant. The particulate ink prepared by this invention has good printability, overcoming the significant challenge of 3D printing hard tissue microparticles.

This invention belongs to the field of biomedical technology and discloses a bone-targeting platform based on black phosphorus and amorphous calcium carbonate, its preparation method, and its applications. In this invention, amorphous calcium carbonate (ACC) is coated onto the surface of bone apatite (BP) to form the core (BA) of a nano-therapeutic platform. Subsequently, a polydopamine (PDA) layer is coated on the surface, and a bone-targeting ligand (polyacrylic acid-aldehyde PA) is further coupled to the PDA layer. After BA@PDA-PA precisely accumulates in bone tissue, it reacts to the acidic microenvironment and undergoes "autolysis-like" degradation, rapidly releasing BP and calcium carbonate. 2+ Released BP can scavenge ROS in the bone microenvironment and improve the bone immune microenvironment. Furthermore, BP and Ca... 2+ As a "raw material" for bone matrix mineralization, it synergistically promotes osteoogenesis and inhibits osteoclast activity, ultimately achieving a precise and multi-effect treatment for osteoporosis (OP).