55 results about "Bone matrix" patented technology

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to an injectable mineralized collagen bone repair composite material loaded with stem cell recruitment factors and osteogenic induction factors and a preparation method of the injectable mineralized collagen bone repair composite material. The composite material is composed of 50%-53% of mineralized collagen matrix particles, 15%-20% of SDF-1alpha loaded heparinized gelatin microspheres, 15%-20% of BMP-2 loaded PDA coated mesoporous silica particles, 8%-10% of an NHS-PEG-NHS cross-linking agent and 4%-6% of glycerin. The composite material can realize time-space sequential regulation and control of'collection first and then differentiation '; a bionic bone matrix microenvironment is provided through the mineralized collagen matrix; the problems of two-factor release conflict and stability are solved; in-vitro experiments show that the material can significantly promote stem cell migration and osteogenic differentiation, and animal experiments prove that the material can recover 82-88% of mechanical properties of bone defect parts. The invention solves the technical problems of insufficient stem cell collection, low osteogenic differentiation efficiency and uncontrollable factor release of the existing bone repair material.

The invention discloses a polysaccharide-tea polyphenol-metal compound, which is formed by dispersing a metal-tea polyphenol nano ligand in polysaccharide gel formed by covalent crosslinking of atractylodes macrocephala polysaccharide and sodium alginate, and the metal-polyphenol nano ligand is a nano complex formed by coordination of tea polyphenol and metal ions. The invention also discloses a preparation method and application thereof. The rhizoma atractylodis macrocephalae polysaccharide-sodium alginate-tea polyphenol derivative-metal composite system disclosed by the invention not only can improve the stability and release behavior of tea polyphenol in vivo, but also can regulate and control an immunometabolism microenvironment in a bone repair process, induce inflammation resolution and promote angiogenesis and bone matrix deposition, so that the bone repair effect is improved. Multiple synergistic effects of inflammation resistance, oxidation resistance, vascularization promotion and osteogenesis induction are realized, so that repair and regeneration of skull defects are remarkably accelerated, and a new intelligent immunometabolism regulation strategy is provided for repair of complex bone defects.

The purpose of the present invention is to three-dimensionally culture osteoblasts produced with a direct conversion technique, and to provide a three-dimensional bone-like tissue composed of the osteoblasts and bone matrix. The present invention relates to, for example, a method for producing an osteoblast-containing three-dimensional cell mass, comprising: a first step for performing direct conversion from somatic cells of mammals into osteoblasts; a second step for subjecting cells having undergone the first step to high-density culture, and forming a cell sheet containing the osteoblasts; and a third step for obtaining an osteoblast-containing three-dimensional cell mass from the cell sheet obtained in the second step.

A method for modeling temporomandibular joint osteoarthritis, 6-week-old rats are selected, the rats are confirmed to have entered an anesthetized state before surgery, the rat's mandibular incisors are first checked and confirmed to be normal, the first arm of the device is sleeved on one side of the mandibular incisor, and the palatal side of the maxillary incisor is made to touch the slope surface of the second arm of the device, the device is checked and confirmed to not affect the rat's mandibular movement and occlusion, not significantly raise the bite, have no early contact, and the upper and lower jaw relationship is relatively stable; the device and the mandibular incisor sleeved with the device are fixed; then the rat is put back into the cage, and soft food or powdered feed is given after waking up; the device is removed after being fed for 8 weeks or more. It is verified that the condylar process of the rat changes significantly, the overall condylar process is significantly reduced, the subchondral bone is destroyed, and the expression of the cartilage matrix is also reduced. Thus, a TMJOA rat model exhibiting mandibular retrusion is formed.

The invention discloses a method for improving mineralized collagen performance based on discrete increase of ion concentration, and belongs to the technical field of biological material preparation, and the method comprises the following steps: step 1, preparing a reaction stock solution; 2, preparing a dynamically mineralized collagen solution; and step 3, preparing the bracket with dynamically changed ion concentration. According to the method for improving the performance of the mineralized collagen based on discrete increase of the ion concentration, the nonlinear process of mineralization of natural bones is simulated by dynamically regulating and controlling the ion concentration, more inorganic minerals can be attached to the collagen, and the mineralization degree of the prepared collagen is higher on the micron scale; on the macroscopic scale, the mechanical property of the mineralized collagen is better, the mineral substances are more, and the technical support can be provided for preparing the multi-scale bionic bone matrix material.

The application relates to the technical field of biological medicine, and discloses a preparation method of a bone melon extract powder injection and a pharmaceutical composition, which comprises the following steps: bone raw material pretreatment, bone polypeptide extraction, melon seed raw material pretreatment, melon seed polypeptide extraction, stabilizer composition preparation and freeze-dried preparation; through deep defatting and decalcification treatment on the bone raw material, and in combination with specific condition enzymolysis and two-step ultrafiltration purification processes, non-target impurities in the bone matrix can be removed, and the molecular weight distribution range of the bone polypeptide can be controlled, so that the uniformity and batch consistency of the active ingredient of the bone polypeptide are improved. Meanwhile, the compound protease enzymolysis strategy used in the bone polypeptide extraction can synergistically act on the bone protein, improves the polypeptide yield, and lays a foundation for the stable control of the active ingredient proportion in the final pharmaceutical composition.

The invention provides a composite growth factor composition capable of promoting proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, a method and application. The invention provides the composite growth factor composition by simulating a natural secretion mode of a decalcified bone matrix of the mandible of an SD (Sprague Dawley) rat on the seventh day, the composite growth factor composition comprises BMP-2 (400-600 pg / ml), TGF-beta1 (5-7 ng / ml) and IGF-1 (150-250 ng / ml), and the optimal concentrations are 500 pg / ml, 6 ng / ml and 200 ng / ml respectively. The composition is remarkably superior to a single factor and a high-dosage combination in the aspects of promoting BMSCs proliferation, improving the activity of alkaline phosphatase (ALP) and forming mineralized nodules, meanwhile, the use dosage of growth factors is remarkably reduced, and the risk of side effects such as heterotopic ossification is reduced. The composite growth factor composition has good biocompatibility and safety, and is suitable for the fields of bone tissue engineering, bone defect repair and related regenerative medicine.

The application provides an application of miR-30d-5p and CYP24A1 inhibitor in hormone-induced femoral head necrosis, and belongs to the technical field of biological medicine.The application firstly provides a hormone-induced femoral head necrosis treatment strategy based on miR-30d-5p mimic by determining the molecular mechanism of miR-30d-5p in the regulation of CYP24A1 in the vitamin D metabolic pathway, and the direct effect is to significantly promote osteoblast differentiation and bone matrix mineralization, effectively improve bone microstructure damage and delay the femoral head collapse process; in the application significance, the application provides a new solution to the predicament of the lack of targeted drug treatment in clinic, and lays a conversion foundation for conservative treatment and hip-preserving surgery combined treatment by blocking the disease progression through specific molecular intervention.

The application provides a traditional Chinese medicine granule for improving osteoporosis and a preparation method and application thereof, and belongs to the technical field of medicines. Codonopsis pilosula, drynaria rhizome, epimedium and eucommia are subjected to deep eutectic solvent extraction, purification, and preparation of a traditional Chinese medicine extract, water extraction, dialysis and drying of pilose antler to prepare pilose antler protein peptide; water extraction and alcohol precipitation of cistanche to prepare cistanche polysaccharide; after modification of hydroxyapatite by citric acid, sodium dihydrogen phosphate and beta-cyclodextrin, the product is prepared by inclusion of calcium salt, alpha-cholecalciferol and vitamin D3, and then mixed with the traditional Chinese medicine extract, the pilose antler protein peptide and the cistanche polysaccharide to prepare the traditional Chinese medicine granule for improving osteoporosis. The prepared traditional Chinese medicine granule for improving osteoporosis can promote the synthesis of collagen and bone matrix protein, inhibit bone resorption, promote bone remodeling and enhance the activity of osteoblasts, and has a good improvement effect on osteoporosis, bone fracture and other symptoms, and has a wide application prospect.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 μm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The application relates to an anti-inflammatory and cartilage regeneration promoting material, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The anti-inflammatory and cartilage regeneration promoting material comprises a PLGA (50:50) polymer material, and a huperzine A derivative dimer loaded on the PLGA (50:50) polymer material through 3D printing. The application also provides the use of the anti-inflammatory and cartilage regeneration promoting material in the preparation of anti-inflammatory, cartilage protection or cartilage regeneration promoting drugs. The PLGA / A10E scaffold has good biocompatibility, the PLGA / A10E scaffold degradation release liquid can effectively promote the differentiation of cartilage precursor cells into cartilage, and increase the formation of cartilage collagen matrix and cartilage proteoglycan. The in-vitro degradation liquid of the PLGA / A10E scaffold can promote the expression of SOX9 under non-inflammatory conditions; under LPS-mediated inflammatory conditions, the expression of MMP-13 can be inhibited, thereby promoting the synthesis of cartilage matrix and inhibiting the degradation of cartilage matrix.

The invention discloses an interbody fusion cage based on a Thiessen polygonal gradient porous structure, and belongs to the technical field of biomedical engineering and orthopedic implants. The fusion cage body structure is of a three-dimensional communicated porous structure, the basic topological configuration of the porous structure is a Thiessen polygon, and the porous structure at least has gradient change in the distribution direction from the outer surface to the interior of the porous structure. The outer-layer small hole structure is beneficial for blood-source factors and osteoblasts to quickly enter and attach, so that osteogenesis is started; the inner-layer macroporous structure provides sufficient space for cell deep migration, blood vessel growth and new bone matrix deposition, sufficient osseointegration from outside to inside is finally achieved, the structure highly simulates a natural bone trabecula network, cell siphon attachment and internal connectivity are synergistically optimized through gradient pore design, and a specific post-treatment process is combined, so that the natural bone trabecula network structure is obtained. And the osseointegration performance and safety of the fusion cage are improved.

The invention discloses a bone-blood vessel coupling regeneration material based on S1P modified decellularized fish scale bone meal as well as a preparation method and application thereof, and belongs to the technical field of biology. After the fish scales are treated by a decellularization method, sphingosine-1-phosphoric acid (S1P) is efficiently anchored on the surfaces of the fish scales by utilizing a cationic gelatin middle layer, and a novel bone-blood vessel coupling regeneration material is developed. The bone-blood vessel coupling regeneration material retains natural components and topological structures of fish scales, and can guide ordered deposition of calcium and phosphorus to improve bone matrix density and compression strength; the endogenous bone marrow mesenchymal stem cells and vascular endothelial cells are actively collected to migrate to the center of a defect cavity by loading the S1P to simulate the homing principle of natural stem cells in an organism, osteogenesis and angiogenesis signal channels are synchronously activated, and endogenous bone-blood vessel coupling regeneration is rapidly started. The invention provides an effective strategy for regulating and controlling osteogenesis-angiogenesis coupling for the field of bone injury repair.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 μm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

The invention relates to the technical field of gene engineering, particularly discloses application of an NSUN2 gene in preparation of a product for diagnosing or treating osteoarthritis, and has the technical key points that the invention reveals that the NSUN2 has a potential value of serving as a clinical diagnosis biomarker; meanwhile, by knockdown and overexpression of the NSUN2 and detection of senescence and cartilage matrix synthesis and catabolism related indexes in cartilage cells, the invention finds that the NSUN2 can inhibit synthesis and secretion of cartilage matrix and promote cartilage senescence, and the increase of the NSUN2 is an important factor causing osteoarthritis, which indicates that the NSUN2 is expected to be used as a target to treat osteoarthritis.

The invention provides a tribenzoyl substituted vakognavine type diterpenoid alkaloid, a preparation method and application of the tribenzoyl substituted vakognavine type diterpenoid alkaloid, and a pharmaceutical composition. After rats are treated by the tribenzoyl substituted vakognavine type diterpenoid alkaloid provided by the invention, the quality reduction trend is obviously relieved, and the foot swelling degree and the arthritis score are obviously reduced; bone loss induced by AIA can be effectively inhibited, and the structural integrity of bone trabecula is protected; cartilage matrix degradation can be inhibited, and cartilage erosion damage is relieved; the anti-inflammatory effect can be achieved by inhibiting the expression of key proinflammatory factors in bone tissues.

This application discloses a composition, conditioning agent, and application for protecting bone health. The active ingredients of the composition for protecting bone health include purslane powder, cucumber powder, celery powder, and Solomon's seal powder. This application scientifically combines the four active ingredients to fully leverage their synergistic effects, effectively activating the Runx2 gene and its expression, promoting the production of bone matrix proteins, and significantly intervening in and improving bone discomfort caused by bone matrix protein deficiency. Furthermore, the composition uses naturally derived active ingredients, exhibiting high safety, excellent biocompatibility, and no obvious side effects. The good compatibility between the active ingredients allows for preparation into various dosage forms according to actual needs, making application convenient and with high compliance. Thus, this provides an effective functional active composition for protecting bone health.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

The invention provides Haitesan type diterpenoid alkaloid as well as a preparation method, application and a pharmaceutical composition thereof. After rats are treated by the Haitesan diterpenoid alkaloid provided by the invention, the quality reduction trend is obviously relieved, and the foot swelling degree and the arthritis score are obviously reduced; bone loss induced by AIA can be effectively inhibited, and the structural integrity of bone trabecula is protected; cartilage matrix degradation can be inhibited, and cartilage erosion damage is relieved; the anti-inflammatory effect can be achieved by inhibiting the expression of key proinflammatory factors in bone tissues.

The application discloses a gradient biomimetic PEEK-HA-Col I composite implant and a preparation method and application thereof, relates to the technical field of medical biomaterials, and has the following scheme: a composite implant composed of a load-bearing inner layer, a bioactive intermediate layer and a biomimetic bone matrix layer is constructed; the load-bearing inner layer contains a PEEK matrix, the intermediate layer contains PDA modified interfaces and HA induced and deposited by the interfaces, and the outer layer contains cross-linked modified collagen type I, and a bioactive gradient distribution is formed along the thickness direction; the preparation method and the application of the composite implant in bone repair are simultaneously provided. The application improves the interface bioactivity and the bone integration capability on the basis of maintaining the PEEK load-bearing support, improves the interface structure stability and the bioactive spatial distribution, improves the surface hydrophilicity and the interface microenvironment, is favorable for promoting early osteogenesis and bone defect repair, improves bone fusion and application adaptability in a load-bearing bone implantation scene, and simultaneously considers the synergistic matching of the load-bearing demand and the biological demand of the bone tissue interface.

The invention provides diterpenoid alkaloid in delphinium delphinium as well as a preparation method, application and a pharmaceutical composition of the diterpenoid alkaloid. After rats are treated by diterpenoid alkaloid in delphinium delphinium provided by the invention, the quality reduction trend is obviously relieved, and the foot swelling degree and the arthritis score are obviously reduced; bone loss induced by AIA can be effectively inhibited, and the structural integrity of bone trabecula is protected; cartilage matrix degradation can be inhibited, and cartilage erosion damage is relieved; the anti-inflammatory effect can be achieved by inhibiting the expression of key proinflammatory factors in bone tissues.

This invention discloses a bone repair scaffold material, its preparation method, and its application. The preparation method includes the following steps: preparation of a mineralized collagen core spinning solution; adding mangiferin to a PLGA electrospinning solution and stirring to obtain a shell spinning solution; and using the core spinning solution and the shell spinning solution, obtaining the bone repair scaffold material through coaxial electrospinning. In this invention, PLGA and mangiferin together form the shell of the scaffold structure, and the sustained release of mangiferin provides a guarantee for the anti-inflammatory and angiogenic effects of the scaffold material. Simultaneously, it simulates the fibrous structure of the bone matrix, achieving slow degradation of the core and achieving the goal of long-term promotion of bone repair.

The invention provides C20 type diterpenoid alkaloid, a preparation method and application thereof and a pharmaceutical composition. After rats are treated by the C20 type diterpenoid alkaloid provided by the invention, the quality reduction trend is obviously relieved, and the foot swelling degree and the arthritis score are obviously reduced; bone loss induced by AIA can be effectively inhibited, and the structural integrity of bone trabecula is protected; cartilage matrix degradation can be inhibited, and cartilage erosion damage is relieved; the anti-inflammatory effect can be achieved by inhibiting the expression of key proinflammatory factors in bone tissues.

The invention discloses a demineralized bone matrix fiber as well as a preparation method and application thereof. The method comprises the following steps: firstly, cleaning and disinfecting natural bones separated out of a body, then cutting the natural bones into bone blocks, further degreasing the bone blocks, and cutting the bone blocks into fibers with the length of 1-100mm and the thickness of 0.2-1.4 mm; then placing the bone fibers obtained by cutting in an acidic demineralization solution for demineralization until the calcium content is 0-20%; the method comprises the following steps: demineralizing bone fibers, then reacting with the demineralized bone fibers by using an anionic surfactant for 0.5-3 hours, cleaning, then reacting for 12-18 hours by using a nonionic surfactant, completing two-stage decellularization, cleaning, and freeze-drying for preservation. During clinical use, the demineralized bone fibers which are kept in a freeze-drying manner are mixed with hydrated liquid or mixed with one of sodium carboxymethyl cellulose, gelatin, poloxamer and chitosan to form an ointment substance which is used as a bone defect repairing material.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 µm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

The invention provides traditional Chinese medicine granules for improving osteoporosis as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: performing deep eutectic solvent extraction and purification on codonopsis pilosula, scalded rhizoma drynariae, herba epimedii and eucommia ulmoides to prepare a traditional Chinese medicine extract, and performing water extraction, dialysis and drying on cornu cervi pantotrichum to prepare cornu cervi pantotrichum protein peptide; performing water extraction and alcohol precipitation on cistanche to prepare cistanche polysaccharide; hydroxyapatite is modified by citric acid, sodium dihydrogen phosphate and beta-cyclodextrin, calcium salt, alpha-calciferol and vitamin D3 are included, the prepared product is uniformly mixed with a traditional Chinese medicine extract, cornu cervi pantotrichum protein peptide and cistanche polysaccharide, and the traditional Chinese medicine granules for improving osteoporosis are prepared. The prepared traditional Chinese medicine granules for improving osteoporosis can promote synthesis of collagen and bone matrix protein, inhibit bone resorption, promote bone remodeling and enhance the activity of osteoblasts, have a very good improvement effect on symptoms such as osteoporosis and fracture, and have a wide application prospect.

The invention discloses an intelligent preparation system and method of a bone repair biological material, and relates to the technical field of biomedical materials.The system comprises a raw material pretreatment and conveying unit used for metering, pretreating and independently conveying a calcium phosphate precursor, a decalcified bone matrix, a hydrogel prepolymer, a bone growth promoting factor and PLGA; the nano-microsphere preparation and packaging unit is in communication connection with the raw material pretreatment and conveying unit and used for receiving PLGA and bone growth promoting factors and preparing PLGA nano-microspheres encapsulated with the bone growth promoting factors under the controllable condition, and the particle size of the PLGA nano-microspheres ranges from 80 nm to 500 nm; by means of accurate metering and pretreatment of the intelligent control center on multi-component raw materials, active regulation and control on the particle size of the nano-microspheres and online monitoring and feedback on the mixing reaction process, repeatable and standardized preparation of the bone repair material with the complex formula is achieved.