219 results about "Bone matrix" patented technology

A method for performing percutaneous interbody fusion is disclosed. The method includes the steps of inserting a guide needle posteriorly to the disc space, inserting a dilator having an inner diameter slightly larger than the outer diameter of the guide needle over the guide needle to the disc space to enlarge the disc space, and successively passing a series of dilators, each having an inner diameter slightly larger than the outer diameter of the previous dilator, over the previous dilator to the disc space the gradually and incrementally increase the height of the disc space. Once the desired disc height is achieved, the guide needle and all the dilators, with the exception of the outermost dilator, are removed. An expandible intervertebral disc spacer is then passed through the remaining dilator and positioned in the disc space. The disc spacer is expanded to the required disc height, and then a bone matrix is passed through the dilator to fill the disc space. The dilator is then removed. An expandible intervertebral disc spacer is also disclosed, having a tapered bore that causes greater expansion of one end of the spacer with respect to the other. A kit for performing the percutaneous interbody fusion procedure is also disclosed.

The present invention provides methods of improving the osteogenic and/or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and/or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. Ona assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and/or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and/or chondrogenic activity of human DBM when tested using this assay system.

A device and method to mechanically strengthen bone matrix and deliver tissue reinforcement material to weakened areas in skeletal structures. A preferred embodiment is an orthopedic screw configured to receive and directionally disperse a therapeutic fluid through the screw's channels and apertures.

Bone matrix compositions having nanoscale textured surfaces and methods for their production are provided. In some embodiments, bone matrix is prepared for implantation and retains nanoscale textured surfaces. In other embodiments, nanostructures are imparted to bone matrix wherein collagen fibrils on the surface of the bone matrix have been compromised, thus imparting a nanoscale textured surface to the bone matrix. Generally, these methods may be applied to mineralized or demineralized bone including partially or surface demineralized bone.

A transplant material which is capable of imparting desired mechanical properties, elevating bone tissue repair speed and improving biocompatibility. This transplant material comprises an artificial and biologically inactive material, which is to be implanted in vivo as a substitute for bone tissue, and at least one type of cells selected from among osteoblasts and precursory osteoblasts which are adhered to the surface of the artificial material so that the artificial material is coated with the bone matrix produced by the cells. The artificial material involves not only a biologically inactive material but also a biologically inactive material coated with a biologically active substrate. This transplant material is produced by culturing mesenchymal stem cells collected from a living body to differentiate into at least one type of cells selected from among osteoblasts and precursory osteoblasts and then culturing the cells together with the artificial material to thereby adhere the differentiated cells on the surface of the artificial material and coat the surface of the artificial material with the bone matrix produced by the differentiated cells.

Methods for increasing osteoinductivity and/or surface area of bone material are provided. The methods include providing bone material and dehydrating the bone material with a solvent at its critical point. A useful solvent for critical point dehydrating is carbon dioxide. Critical point dehydration resulting in increased osteoinductivity and/or surface area can be applied to many types of bone material including bone particles, bone chips, bone fibers, bone matrices, both demineralized and non-demineralized. An implantable composition having an enhanced osteoinductivity and/or osteoconductivity is also provided. The implantable composition contains demineralized bone matrix dried at critical point of carbon dioxide. Critical point dried fibers of a demineralized bone matrix have a BET value from about 40 m²/gm to about 100 m²/gm, a value that is 100 times higher than corresponding vacuum dried or lyophilized DBM fibers.

This invention provides a novel medical appliance for repairing, regenerating, maintaining, and/or augmenting a bone. The medical appliance generally includes an osteoinductive agent, an osteoinductive enhancer, and a carrier matrix. Also disclosed are methods, compositions, kits, and bone matrix formulations for regenerating, maintaining, and/or augmenting a bone. Exemplary preferred osteoinductive agents include growth factors such as BMP and TGF-β. Exemplary preferred osteoinductive enhancers include phytoestrogens such as naringin.

Methods for demineralizing bone are provided. In some embodiments, the methods include demineralizing the bone in a demineralization medium and separating the bone into particles during demineralization. In other embodiments, the methods include placing the bone in a demineralization medium and treating the demineralization medium while the bone is in the demineralization medium to optimize the demineralization process.

Bone matrix compositions and, more specifically, demineralized bone matrix (DBM) having increased osteoinductive capacity and methods for its production are provided. Specifically, DBM derived from cortical bone from the periosteal layer of bone are provided. Compositions comprising a disproportionate amount of DBM prepared from bone derived from the periosteal and/or middle layer of bone are provided. Preparations of and methods of use of periosteal DBM compositions are disclosed.

A method for performing percutaneous interbody fusion is disclosed. The method includes the steps of inserting a guide needle posteriorly to the disc space, inserting a dilator having an inner diameter slightly larger than the outer diameter of the guide needle over the guide needle to the disc space to enlarge the disc space, and successively passing a series of dilators, each having an inner diameter slightly larger than the outer diameter of the previous dilator, over the previous dilator to the disc space the gradually and incrementally increase the height of the disc space. Once the desired disc height is achieved, the guide needle and all the dilators, with the exception of the outermost dilator, are removed. An expandible intervertebral disc spacer is then passed through the remaining dilator and positioned in the disc space. Th disc spacer is expanded to the required disc height, and then a bone matrix is passed through the dilator to fill the disc space. The dilator is then removed. An expandible intervertebral disc spacer is also disclosed, having a tapered bore that causes greater expansion of one end of the spacer with respect to the other. A kit for performing the percutaneous interbody fusion procedure is also disclosed.

The invention discloses a method for preparing a tissue-engineered bone/cartilage integrated scaffold. An integrated scaffold structure of which internal pores are aligned and pore diameters are changed in a gradient way along a thickness direction and which has good mechanical properties is obtained by simulating the composition and spatial structure characteristics of natural articular cartilage and using extracellular matrixes and bone matrix gelatin of the cartilage as main raw materials by methods such as a different concentration superposition method, a directional crystallization method, a freeze-drying method, a cross-linking reaction method and the like. The high biocompatibility of the scaffold is favorable for the adhesion and proliferation of cells; a special pore structure of the scaffold ensures that the content of matrixes secreted by the cells is also changed in a gradient way; and meanwhile, a bone matrix gelatin substrate provides good mechanical support for the instant fixation of the scaffold and an injured part.

The invention discloses a cartilage tissue engineering repair bracket and a preparation method thereof, and belongs to the cartilage tissue repair field. The bracket comprises natural high polymer water-gel and decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface; the affinitive peptides of the mesenchymal stem cells are coupled on the surface of the decalcified bone matrix to obtain the cartilage tissue engineering repair bracket with special mesenchymal stem cell affinity. By being placed in a cartilage defective region, the cartilage tissue engineering repair bracket can collect a plurality of BMSC (bone marrow stromal cells); and in the bracket, under mating effect of the natural high polymer water-gel and the decalcified bone matrix, the BMSC can be retained in the cartilage defective region for a long time, so that the cartilage repair has a longer curative effect period. The invention further discloses a preparation method of the bracket, wherein the natural high polymer water-gel aqueous liquor is injected in the decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface to carry out gelation reaction to obtain the bracket with higher mechanical strength and inducibility. The method is simple and high in practicability.

The invention discloses a clinical bone filling, restoring and replacing material in the medical biological material domain, which is characterized by the following: I-typed collagen and Ca<2+> mineralize artificially and assemble automatically, possessing excellent biological compatibility.

Biomaterial having a multi-dimensional structure and comprising differentiated MSCs tissue and demineralised bone matrix, wherein said demineralised bone matrix is dispersed within the differentiated MSCs tissue, method for producing the same and use thereof.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The invention aims at providing a bone animation processing method based on a programmable graphics processing unit (GPU). Calculation tasks of different parts of bone animation are placed into the GPU to enable parts of or all of calculation amounts to be transferred from the GPU to the GPU, and accordingly the GPU is liberated from burdensome vertex transformation and bone update. The bone animation processing method based on the GPU comprises: step one, binding vertexes in the GPU to corresponding bones; step two, binding offset matrixes of the bones and corresponding skeletons in the GPU; step three, updating skeletons in the GPU and performing matrix multiplication to updated skeletons and corresponding offset matrixes and transmitting updated bone matrixes and vertex information to the GPU; and step four, utilizing transmitted bone matrixes to update the vertex information in the GPU.

A novel method for cartilage and bone induction is introduced whereby an artificially perforated bone organic bone matrix augmented by live human undifferentiated cells suspended in a gel is used as a surgical implant.

The invention relates to a bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and a preparation method thereof. The material is an animal bone matrix material with the umbilical cord mesenchymal stem cells inoculated on the surface of a decalcified bone matrix and used for secreting osteogenic induction function proteins to promote osteogenesis. The bone matrix material has natural structures and characteristics of bone tissues and a good osteogenic induction effect, has a better osteogenesis promoting effect in a bone defect repair process, and is helpful for large-scale preparation and clinical application of tissue-engineered bones.

A cell-removed heterogeneous bone matrix is prepared from the rib and extremity bone of pig through physical and chemical processing, and removing cells and the heterogeneous protein from tissue by use of proteolytic enzyme and Triton-X100. Its advantages are natural components and biologic chemical strength, low antigenicity, high compatibility and bone conductivity, and low cost.