70 results about "New Zealand Rabbit" patented technology

The invention discloses a kit for rapid detection of staphylococcus aureus in a sample and a detection method thereof, belonging to the technical field of immunological detection. In the invention, a staphylococcus aureus immunogen inactivated with formaldehyde is used for immunizing a healthy New Zealand rabbit to obtain a polyclonal antibody to serve as a coated antibody, and used for immunizing a BALB/C mouse and performing cell fusion to obtain a monoclonal antibody to serve as a secondary antibody, thus, the kit for performing a double antibody sandwich enzyme-linked immunosorbent assay on the staphylococcus aureus in food (milk) is established, and a rapid and efficient detection means is provided for residual detection of the staphylococcus aureus in the food, and the advantages of lower cost and better stability and repeatability are achieved. A detection limit of the kit is 105cfu/mL and is suitable for detecting mass samples.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The invention relates to an absorbable antibacterial hemostatic microsphere, a preparation method and application of the absorbable antibacterial hemostatic microsphere. The absorbable antibacterial hemostatic microsphere is prepared from carboxymethyl chitosan, sodium alginate, gelatin and lysozyme. The absorbable antibacterial hemostatic microsphere disclosed by the invention can be used for carrying out quick hemostasis on a massive haemorrhage wound and preventing the massive haemorrhage wound from being infected by bacteria; the absorbable antibacterial hemostatic microsphere has good biocompatibility, can be degraded and absorbed and is capable of promoting the wound healing. The invention also discloses the effect of the application of the composite absorbable antibacterial hemostatic microsphere in hemostasis of New Zealand rabbit hemihepatectomy.

The invention relates to a kit used for rapidly detecting Escherichia coli O157:H7 in a sample, and a detection method thereof. The invention belongs to the technical field of immunological detection. According to the invention, a heated and deactivated Escherichia coli O157:H7 immunogen is used for immunizing a healthy New Zealand rabbit, such that a polyclonal antibody is obtained, and the polyclonal antibody is adopted as a coating antibody; a BALB/C mouse is immunized, and cell fusion is carried out, such that a monoclonal antibody is obtained, and the monoclonal antibody is adopted as a secondary antibody; and a double-antibody sandwich ELISA kit of Escherichia coli O157:H7 in foodstuffs (meat) is established. With the kit, a rapid and highly efficient detection means is provided for the detection of the residue of Escherichia coli O157:H7 in foodstuffs. The kit is advantaged in relatively low cost, relatively good stability, and relatively good repeatability. According to the invention, a detection limit is 105cfu/mL. The kit and the method are suitable for large-batch detections of samples.

The invention relates to a rhodamine B ELISA detection method, which belongs to the technical field of ELISA. The invention discloses a rhodamine B ELISA detection method, which comprises the following steps: using synthesized rhodamine B immunogen for immunizing healthy New Zealand rabbits to obtain polyclonal antibodies; and using rhodamine B as standard products; using rhodamine B hapten and OVA coupling materials as coating antigen to establish a rhodamine B indirect ELISA method. The invention provides the fast and efficient detection method for the residual detection on the rhodamine B. Because the polyclonal antibodies are adopted, the cost is low, and in addition, the stability and the repetitiveness are good. The detectability (IC90) is 0.028 ng/mL, the half inhibitory amount (IC50) is 1.3 ng/mL, and the detection range (IC20 to IC80) is between 0.07 and 17.5 ng/mL. Because of high specificity and high compatibility of the immune reaction, high selectivity and high sensitivity can be obtained when the ELISA is used for detecting the rhodamine B.

The invention relate to a immunity antibody for detecting polyether antibiotic remains and the application of the antibody. With the monensin sodium salt as half antigen, first reform the half antigen and then couple the reformed half antigen with protein producing artificial immunogen, and obtain multi clone antigen by immunizing the New Zealand rabbit or single clone antigen by immunizing the small mouse, cell fusion, sieving, clone cultivation. The obtained antigen can be used for test the animal source food on polyether antibiotic remains. The advantages of the invention are that the antigen obtained by immunizing animals with the coupling product of reformed monensin sodium salt and protein, has got specificity on polyether antibiotic medical which can be applied on polyether antibiotic remain detecting in animal source food. The detection adopted with this invention is fast and convenience.

The invention discloses a vitamin B2 conjugate. The vitamin B2 conjugate is formed by coupling vitamin B2 hapten and bovine serum albumin or ovalbumin which generates immunogenicity and is a carrier material, wherein n refers to number of molecules, of vitamin B2, combined with one bovine serum albumin molecule and is an integer from 1-20, BSA refers to bovine serum albumin, and molecular weight ranges from 6.6KDa to 6.9KDa. The invention further discloses a preparation method of the vitamin B2 conjugate. The preparation method includes: connecting vitamin B2 with the carrier material generating immunogenicity to form the coupling material capable of inducing an animal immune system to generate antibodies. Antiserum with titer reaching 1:256000 is prepared through immune New Zealand rabbits, and limit of detection is 1.07ng/ml. The preparation method has the advantages of simplicity, convenience, quickness, specificity and accuracy. A foundation is provided for manufacturing an enzyme-linked immunosorbent assay reagent box of vitamin B2.

The invention discloses a method for establishing an animal model infected with Acinetobacter baumannii pneumonia. The method comprises the following steps: (1) preparing an immunosuppressive New Zealand rabbit; (2) preparing an Acinetobacter baumannii solution; (3) infecting the animal and establishing the model. The simple, rapid and efficient animal model infected with Acinetobacter baumannii pneumonia is established to help people research production and development processes of drug resistance of Acinetobacter baumannii and drug resistance related mechanisms of the Acinetobacter baumannii, assistance is provided for delaying of drug resistance of bacteria and better treatment of infection with the Acinetobacter baumannii, expense for related treatment of patients can be reduced, and human health can be promoted.

The invention discloses a toxoplasma gondii TgVP1 extracellular region antigen polypeptide, an anti-toxoplasma gondii TgVP1 polyclonal antibody and the application of the polyclonal antibody. The amino acid sequence of the toxoplasma gondii TgVP1 extracellular region antigen polypeptide is as shown in SEQ ID NO: 1 or a sequence as shown in SEQ ID NO: 1 of which the N end is connected with a cysteine. A New Zealand rabbit is immunized by the toxoplasma gondii TgVP1 extracellular region antigen polypeptide as an immunogen so that the anti-toxoplasma gondii TgVP1 polyclonal antibody can be obtained; an identification result indicates that the polyclonal antibody is capable of specifically identifying the toxoplasma gondii TgVP1 protein and can be applied to the detection of the toxoplasma gondii TgVP1 protein in tests such as ELISA, Western blot and immunofluorescence. A powerful tool is provided for the fundamental research of the protein functions and the research of the protein as a potential anti-toxoplasma gondii drug target; in short, the polyclonal antibody is wide in application prospect.

The invention discloses a toxoplasma gondii TgVP1 extracellular region antigen polypeptide, an anti-toxoplasma gondii TgVP1 polyclonal antibody and the application of the polyclonal antibody. The amino acid sequence of the toxoplasma gondii TgVP1 extracellular region antigen polypeptide is as shown in SEQ ID NO: 1. A New Zealand rabbit is immunized by the toxoplasma gondii TgVP1 extracellular region antigen polypeptide as an immunogen so that the anti-toxoplasma gondii TgVP1 polyclonal antibody can be obtained; an identification result indicates that the polyclonal antibody is capable of specifically identifying the toxoplasma gondii TgVP1 protein and can be applied to the detection of the toxoplasma gondii TgVP1 protein in tests such as ELISA, Western blot and immunofluorescence; a powerful tool is provided for the fundamental research of the protein functions and the research of the protein as a potential anti-toxoplasma gondii drug target; in short, the polyclonal antibody is wide in application prospect.

The invention belongs to the field of biology and particularly relates to a method applicable to in-vitro mature culture of young rabbit oocytes and a formulation of a culture medium. According to the method, a cumulus-oocytes complex is cultured in vitro by using a TCM-199 basic culture system; based on a TCM-199 culture medium, 10% of FBS, 0.11 g/L of sodium pyruvate, 10 Mug/ml of LH, 10 Mug/ml of FSH, 1 Mug/ml of E2, 100 IU/ml of penicillin, 100 IU/ml of streptomycin sulfate and 15-20 Mum of epigallocatechin gallate (EGCG). According to the invention, the EGCG is added into the TCM-199 culture medium to study the influence of the EGCG on in-vitro maturation of young New Zealand rabbit oocytes, so that a simple and practical method for in-vitro maturation of the young rabbit oocytes is established.

The invention relates to a nontoxic ST28 streptococcus suis and an application thereof, and belongs to the biotechnology field. A tonsil sample of a healthy slaughter pig is collected; after bacteria enrichment processing, bacteria DNA (deoxyribonucleic acid) is extracted; a cps2j gene is identified by polymerase chain reaction; then, pure culture bacteria can be obtained by bacteria plate separation; the pure culture bacteria is subjected to gram stain, serum agglutination, polymerase chain reaction and multilocus sequence typing to determine and separate type-2 bacterial strain of the streptococcus suis and a sequence type thereof; animal experiments of a Balb/c mice, a New Zealand rabbit and a Landrace piglet prove that the bacteria HA0609 is a nontoxic bacterial strain; after the bacteria is subjected to intramuscular injection into the New Zealand rabbit for one time and is subjected to intraperitoneal injection into a CD1 mice for two times, certain immunocompetence can be provided for an immunized animal, and analysis on a HA0609 immune protection antigen can be used for developing streptococcus suis disease subunit vaccine.

The invention discloses a porcine P21 protein antibody as well as a preparation method and an application thereof. Porcine P21 protein obtained through expression and purification is used for preparing an immunogen to immunize a purebred New Zealand rabbit so as to prepare a rabbit anti-pig P21 protein antibody; and the prepared rabbit anti-pig P21 protein antibody is capable of well recognizing the P21 protein of a pig and a mouse, while a commercially available anti-human P21 antibody is only capable of limitedly recognizing the P21 protein of the mouse. The porcine P21 protein antibody prepared in the invention fills up the blank in deficiency of a porcine specific antibody and provides materials for a scientific research of porcine genetic breeding.

The invention relates to a multi-walled carbon nanotube injected with carboxyl ions. The preparation method comprises the following steps: spraying the multi-walled carbon nanotube on a silicon dioxide substrate which is used for pre-baking carbon membranes, and injecting the carboxyl ions in the surface of the carbon nanotube after being sprayed by an ion implanter, wherein the implanting density of the carboxyl ions is 5*1013 in each square centimetre, 1*1014 in each square centimetre and 5*1014 in each square centimetre; the ion energy is 40 keV; and the percentage of the oxygen atoms in the material is 7.53%-12.86%. The experiment of adhering platelets of adult New Zealand rabbits on the material shows that anti-coagulant properties of the material can be effectively improved through injecting the carboxyl ions on MWCNTs, so the material has good applicable value of researches and wide application prospect while being adopted as material contacted with blood.

The invention discloses a method used for preparing a New Zealand rabbit scald model, which comprises the steps as follows: medical gauze is used as a hot carrier; firstly, the optimum gauze layer number, by which the heat can be continuously kept, is determined; the gauze which is heated to 99 DEG C in constant-temperature water is respectively used for scald the back of the New Zealand rabbit AT by 12 time points of 3-25s; samples are obtained 24 hours after the scald; the scald degree is evaluated by HE staining; furthermore, corresponding analysis of the time and degree of scald is carried out; scald models as follows are established according to different scald time: the scald model of level-I with the scald time of 3-5s, the scald model of level-shallow-II with the scald time of 7-13s, the scald model of level-deep-II with the scald time of 15-19s, and the scald model of level-III with scald time of 21-25s. The method of the invention has the advantages of exact control of scald area and depth, simple and convenient operation and good repeatability, and the model prepared by the method is a good model used for researching scald restoration mechanism.

The invention provides a ciprofloxacin propionate hapten, a preparation method of the ciprofloxacin propionate hapten, application of the ciprofloxacin propionate hapten, and a ciprofloxacin antigen prepared from the ciprofloxacin hapten. A New Zealand rabbit is immunized with the ciprofloxacin antigen, so that an antibody with high specificity can be obtained.

The invention relates to a preparation method of an HPV58E6 specificity rabbit monoclonal antibody. The preparation method comprises the following steps: designing an HPV58E6 specificity polypeptide segment; synthesizing the polypeptide segment and crosslinking with two vectors; immunizing a New Zealand rabbit with the synthesized polypeptide antigen, measuring serum titer and detecting the antibody specificity through Western Blot; then carrying out cell fusion culture on splenocyte and fusion partner cells; taking supernatant and detecting antibody titer and specificity; screening out fusion cells with the best specificity and titer; carrying out fusion cell lysis to extract RNA, and carrying out RT-PCR to obtain cDNAs of heavy and light chains of the antibody so as to establish a pTT5 plasmid vector; and co-transfecting the plasmid containing the heavy and light chains to an HEK-293-6E cell to be expressed to finally obtain a recombinant antibody, namely HPV58-E6 monoclonal antibody, wherein the antibody can be applied to Western Blot for detecting expression of HPV58 type oncogene E.

The invention discloses a method of the in-vitro expression of a gekko japonicus Hoxc10 (homebox gene c10) protein and the preparation of a polyclonal antibody, comprising the following steps of: obtaining an EST (Expressed Sequence Tag) sequence of gekko japonicus Hoxc10; obtaining an overall length sequence of the gekko japonicus Hoxc10 through an RACE (rapid amplification of cDNA ends) method; predicting an antigenic epitope of the gekko japonicus Hoxc10 protein on line by adopting DNASTAR software and SWISS-PLOT; constructing a prokaryotic expression vector of the gekko japonicus Hoxc10 by selecting superior epitope peptides; inducing the expression of fusion proteins in vitro; purifying the fusion proteins; preparing the polyclonal antibody, and the like. In the invention, an in-vitro gekko japonicus Hoxc10 expression system constructed on the basis of pGEX-4T1 can efficiently express a target protein in BL21 and further purify the target protein; and a large quantity of GST-Hoxc10 fusion proteins can be conveniently obtained; in addition, a rabbit gekko japonicus Hoxc10 antiserum prepared by immunizing a New Zealand rabbit through the GST-Hoxc10 fusion proteins has high titer and good specificity.

The invention relates to a polyclonal antibody for efficiently recognizing proteinserine heptanose glycosylation, and a reparation method and application thereof. The antibody is used for detecting different structures of natural protein modified by proteinserine heptanose glycosylation and screening and discovering novel antibiotics. The preparation process of the antibody comprises the steps offirstly building a C7 sugar framework by Wittig reaction; then, obtaining six-bit heptose of two absolute structures through Sharpless asymmetrical oxidization reaction; under the help of glucosinolates and trichloroacetimidate mediated glycosylation reaction, obtaining proteinserine heptanose with the same glycosylation modification structure in the organism; then, obtaining the corresponding glycopeptide with the sequence being Ac-GS(Hep)GL-OH by using a polypeptide solid phase synthesis method; coupling the obtained semi-antigen onto the carrier protein BSA to obtain antigen; immunizing animals by the antigen; collecting immunized New Zealand rabbit blood to prepare antiserum; obtaining IgG through affinity purification. The prepared antibody has high specificity and high valence.

The invention belongs to the technical field of bioengineering, and relates to BACE1 shearing high-tilter antibodies prepared by using a GST expression system, and an application of the antibodies. BACE1 shearing gene is cloned into a prokaryotic expression vector by using a gene engineering technology, enzymatic detection identification is carried out, Escherichia coli is converted by using recombinant plasmids, IPTG induction is carried out to generate GST-BACE1146-189aa and GST-BACE1190-2144aa fusion proteins, purified fusion proteins are used to immunize New Zealand rabbits in order to prepare antiserum, and the antiserum is purified by using ProteinA/G. The two anti-BACE1 shearing polyclonal antibodies have good titer and specificity, can be applied to the detection of different BACE1 shearing types 52 KD and 49 KD of in cells and the distinguishing of four shearing types, can effectively detect the differential expression of the BACE1 shearing types in different cells, and is of great significance to systemically research the functions of the BACE1 and the action mechanism of the BACE1 in AD.

The invention discloses an antigenic polypeptide of poplar functional centromere histone CENH3 and application thereof, belonging to the fields of bioinformatics and molecular cytogenetics. The invention mainly comprises the following steps: screening a specific amino acid sequence of populus tomentosa CENH3 protein and artificially synthesizing a polypeptide; immunizing New Zealand rabbits with the polypeptide to prepare antiserum; and carrying out affinity purification to obtain a CENH3 protein antibody. Immunofluorescence detection and ChIP-FISH are carried out on the antibody to verify thespecificity, thereby proving that the antibody can accurately identify the functional centromere. The antibody can be universally used in poplar species, can accurately mark the position of the poplar functional centromere, and lays a foundation for developing the research on the structure, function and evolution of the poplar functional centromere. The invention has wide application prospects inthe research fields of poplar molecular cytogenetics, genomics, epigenomics and the like.