119results about How to "Improve anticoagulant performance" patented technology

The invention relates to a highly ticoagulant cellulose membrane material, wherein an amphichroic ionomer layer is grafted on the surface of a cellulose membrane, and a 'brush'-shaped amphichroic ionomer layer is formed on the surface of the cellulose membrane. The cellulose membrane with good biocompatibility is prepared by grafting of amphichroic ionic molecules on the surface of the material. The neat 'brush'-shaped amphichroic ionomer layer is formed on the surface of cellulose, so that the defects on the surface of the material are overcome, and the action between the material and biomacromolecules is further reduced, thereby nonspecific protein absorption and blood platelet adhesion are reduced to the maximum degree. Therefore, the cellulose membrane material has high nonspecific protein absorption resistance and high ticoagulant performance. The invention discloses a method for manufacturing the highly ticoagulant cellulose membrane material.

A Chitosan-arginine conjugate as anticoagulant material is prepared through dissolving chitosan in the solution of N, N, N'-tetramethyl ethanediamine, adding 1-ethyl-3-(3-dimethylamino propyl) carbodiimine, N-hydroxy-butanediimide and arginine, reacting while magnetic stirring, dialyzing, drying to obtain said conjugate, adding glucosyl aldehyde oxide, and cross-linking reaction to become film. Its advantages are high effect and low poison to cell.

The invention relates to the field of tissue engineering, in particular to a preparation method of a composite nanometer fiber small-diameter intravascular tissue engineering stent material. The technical scheme is achieved as follows: formic acid is used as a common solvent; RGD-recombinant spider silk protein, polycaprolactone and chitosan are mixed in a mass ratio of 1:8:1 according to the requirements of an intravascular stent to prepare a spinning solution with the concentration of 20-30 percent (w/v); electrospinning process parameters are as follows: the diameter of a cross section of acylindrical rotating shaft is 3-6 mm, the rotating speed is 1,500-3,000rpm, the curing distance is 15-30 cm, the voltage is 50-150kV, the extrusion speed is 3-7ml/h and the temperature is 45-50 DEG C. The small-diameter intravascular stent has favorable cell adhesion-promoting capability, anticoagulant property and mechanical property for resisting physiological environment and can be clinicallyapplied as a small-diameter intravascular tissue engineering stent.

The invention discloses an anticoagulation coating material and a preparation method thereof. The anticoagulation coating material comprises sodium hyaluronate and hirudin, wherein the sodium hyaluronate is formed into a gel network; the hirudin is in covalent binding with the sodium hyaluronate. The preparation method of the anticoagulation coating material comprises the following steps: (1) preparing an MES (Fatty Acid Methyl Ester Sulfonate) buffer solution; (2) preparing a sodium hyaluronate solution with the concentration of 1mg/ml to 20mg/ml; (3) preparing a hirudin solution with the concentration of 1mg/ml to 10mg/ml; (4) uniformly mixing up the two solutions so as to enable the mass ratio of the hirudin to the sodium hyaluronate to be 1:1 to 1:10; (5) adding a crosslinking agent into the mixture solution obtained in the step (4), uniformly mixing up so as to covalently crosslink the hirudin and the sodium hyaluronate. The anticoagulation coating material disclosed by the invention is high in anticoagulation activity, stable in effect, long in lasting time, good in biocompatibility, simple in preparation method, gentle in condition and applicable to large-scale industrial production.

The present invention belongs to the field of anticoagulant material technology. The anticoagulant surface modification process of artificial implant includes the following steps: cleaning artificial implant; preparing silicone rubber solution; coating the artificial implant with medical silicone rubber as support; coating perfluoro sulfonic acid on the surface of the silicone rubber coating; depositing polycation and polyanion alternately onto the surface of the perfluoro sulfonic acid coating via electrostatic attraction; and irradiating the artificial implant with ultraviolet light to produce photochemical reaction to convert the ionic bond between coating layers into covalent bond. The present invention has simple technological process, mild reaction condition, easy operation, high repeatability, environment friendship and thus excellent application foreground.

The invention provides a drug-loading intravascular stent which comprises a stent body and a drug coating located on the stent, wherein the outer surface of stent coating is arranged as a smooth mirror surface; the coating comprises a first coating, a second coating and a third coating in turn from the surface of the stent body to the outer side; dopamine is used as a coating carrier of the first coating and is grafted with a substance A; PLGA is used as the coating carrier of the second coating; the second coating comprises a substance B; PLGA is used as the coating carrier of the third coating; the third coating comprises drugs C and B; A is heparin, B is sirolimus and C is curcumin. The invention also provides the preparation method for the stent. The method comprises the following steps: treating the stent; preparing a freeze-drying material; preparing the first coating; preparing the second coating; preparing the third coating. The invention has the advantages that three-layer drug loading coatings are arranged, the drugs are released layer by layer in the sequence of sirolimus, curcumin and heparin after the stent is implanted, the sequence is conformed to the occurrence trend of thrombus and smooth muscle proliferation, the effective drug duration is prolonged and the inhibiting effect for the subsequent potential thrombus and smooth muscle proliferation is achieved.

The invention provides a coating method for an activated carbon adsorbent for blood perfusion. Firstly, coating film is formed on the surface of activated carbon with chitosan, then crosslinking is executed with epichlorohydrin to increase the strength of the film, and finally heparin sodium having an anticoagulant effect is grafted to the surface of the film. The crosslinked chitosan film can improve the blood compatibility of the activated carbon and decrease particle falling of the activated carbon; the heparin sodium having an excellent anticoagulant property is grafted on the film so thatthe blood coagulation phenomenon occurring when blood is in contact with the surface of the activated carbon can be decreased, and the safety of a perfusion device is improved.

The invention belongs to the field of biological materials, and particularly relates to an anticoagulant composite hollow fiber membrane and a preparation method thereof. The fiber membrane comprisespolyethersulfone, maleylated chitosan and a water-soluble polymer pore-forming agent, wherein the polyethersulfone is in a porous grid framework structure, and after being modified by the maleylated chitosan, the biocompatibility of the polyethersulfone is increased, the platelet aggregation is reduced, and the anticoagulant performance is improved; by adding the water-soluble polymer pore-formingagent, on one hand, the inner diameter, pore diameter and porosity of the fiber membrane can be adjusted, so that the permeability of the fiber membrane is controlled; on the other hand, the water-soluble polymer pore-forming agent is embedded in the porous grid framework structure and is matched with the maleylated chitosan, so that the stability is good, the water-soluble polymer pore-forming agent is not easy to elute and can be used for a long time. The fiber membrane is used as a dialysis membrane to carry out hemodialysis on a patient without using heparin, so that the diseases such asosteoporosis, low coagulation performance and the like of the patient are avoided. The preparation method is simple and suitable for industrial production.

The invention discloses a lysine polyurethane medical material with the surface modified by carboxymethyl chitosan. A base layer is formed with a medical polyurethane material or a synthesized polyurethane material, and a modification layer is formed on the surface of the base layer through a chemical modification method. The modification layer contains carboxymethyl chitosan linked with a polyurethane substrate material through a covalent bond and lysine linked with carboxymethyl chitosan through a covalent bond by means of an active group on carboxymethyl chitosan. The invention further discloses a preparing method of the lysine polyurethane medical material with the surface modified by carboxymethyl chitosan. Reactivity points are generated on the surface of polyurethane through pre-activating treatment, then polyurethane and carboxymethyl chitosan are grafted and copolymerized, and the hydrophilia and biocompatibility of polyurethane as the medical material are improved; by introducing a second modification layer of lysine to the system, the anticoagulation property of polyurethane as the medical material is improved, production is simple, cost is low, and industrial popularization is easy.

The invention discloses a micro-nano multi-scale patterned anticoagulation composite biological material and a method for preparing the same. The micro-nano multi-scale patterned anticoagulation composite biological material comprises a titanium dioxide (TiO2) nano-wire array and an amorphous carbon film wrapping the top end of the nano-wire array. The method for preparing the anticoagulation composite biological material includes the steps: firstly, arranging micron-sized patterns on a fluorine-doped tin oxide (FTO) conductive glass substrate through the mask plate photoetching technology, secondly, enabling the TiO2 nano-wire array to grow on the patterned substrate by means of hydro-thermal synthesis, and enabling the TiO2 nano-wire array to grow on a micron-sized patterned area, so that the whole TiO2 nano-wire array is in a micro-nano patterned structure, and finally, wrapping the amorphous carbon film on the prepared micro-nano patterned array. By the aid of composition of two materials and the design of the micro-nano patterned structure, properties such as surface appearance, hydrophilicity and hydrophobicity, electron transfer characteristic and the like of the materials can be changed and controlled, and blood compatibility of the composite material is improved.

The invention relates to the field of medical devices, in particular to a preparation method of a surface-heparinized anticoagulation medical device based on ionic bond-covalent bond synergistic action. The method comprises the following steps: firstly, fixing heparin on the surface of a medical device by utilizing a hydrophilic polymer modified by low-molecular-weight hyperbranched polyethyleneimine as a side chain in an ionic bond combination manner; and furthermore, grafting a stable heparin coating through covalent bonds, so as to obtain the anticoagulant biomedical device with high anticoagulant activity and high anticoagulant stability. The device has a wide application value in the field of biomedical treatment. The method is simple in preparation process, high in controllability and low in material price, does not involve toxic reagents in the experiment process, has universality for various medical device structures, and is suitable for industrial production.

The invention discloses modified polycarbonate with high anticoagulant activity. Modified polycarbonate is a linear macromolecular polymer, wherein a side chain of the polymer contains functionalizeddouble bonds and hydrophilic phosphorylcholine groups. Modified polycarbonate comprises copolymers A, B and C. The preparation process of modified polycarbonate comprises the following steps: copolymerizing TMC (trimethylene carbonate) and a trimethylene derivative firstly, and then grafting the side chain of the copolymer with MPC (2-methacryloyloxyethyl phosphorylcholine) through a chick reaction of dithiol and the double bonds to obtain the target product modified polycarbonate with high anticoagulant activity. According to modified polycarbonate with high anticoagulant activity, performance of polycarbonate can be changed by adjusting monomer types and proportion and quantity of the phosphorylcholine groups on the side chain, and the anticoagulant capability of polycarbonate as a biodegradable medical polymer material can be improved; reaction sites can be provided by the double bonds on the side chain for follow-up grafting with biomacromolecules or other functional molecules, theresearch potential is high, and the derivative performance is wide.

The invention belongs to the field of biological materials and discloses a surface-heparinized cellulose ester liquid crystal material and a preparation method and application thereof. The preparation method of the liquid crystal material comprises the following steps of: (1) preparation of acryloyl-octanoyl-hydroxy propyl cellulose ester liquid crystal: dissolving hydroxy propyl cellulose into an acetone solution, adding acryloyl chloride and reacting; after the reaction is over, cleaning the obtained mixed solution for 3-5 times, and dialyzing and drying; dissolving the obtained product in acetone, dropwise adding octanoyl chloride and reacting; and cleaning, dialyzing and drying to obtain acryloyl-octanoyl-hydroxy propyl cellulose ester; and (2) preparation of surface-heparinized cellulose ester liquid crystal material: dissolving the acryloyl-octanoyl-hydroxy propyl cellulose ester liquid crystal in tetrahydrofuran, fetching the solution to form a film, then adding a sodium hydroxide solution of heparin sodium, reacting and cleaning to obtain the surface-heparinized cellulose ester liquid crystal material.

The invention relates to a biodegradable polymer containing phosphorylcholine (PC) and polyethylene glycol (PEG) and a synthetic method thereof. The synthetic method comprises: MPC with the mass ratio of 1-99% and PEG-PLA which is connected with double linkage and has the mass ratio of 99-1% are dissolved in trichloromethane and then added with free radical polymerization initiator to react for 6-24h at the temperature of 0-80 DEG C, and the products are precipitated by methyl alcohol and dried in vacuum; the PEG-PLA which is connected with double linkage is prepared by ring opening polymerization of lactide initiated by the PEG that reacts with acryloyl chloride at a single end. The phosphorylcholine group which has positive and negative charges and is introduced on the PLA of the polymer can be seen according to the static contact angle result, and then the contact angle is obviously reduced, so that hydrophilicity is greatly improved. The higher the MPC content is, the smaller the contact angle is, and the better the wettability of the material is. Test results of anticoagulation prove that the PEG can reduce coagulation of blood platelet, affects prothrombin time (PT) and effectively avoids the activation of an extrinsic coagulation system; the high content PC group can effectively reduce the conglutination of the blood platelet and has influence on anginal partial thromboplastin time (APTT) of the activation part.

The invention provides a hypotensive drug and a preparation method thereof. The hypotensive drug comprises the following components in parts by weight: 200-400 parts of kudzuvine root, 200-400 parts of red sage root, 80-150 parts of ligusticum wallichii, 200-400 parts of astragalus, 100-250 parts of cassia seed, 200-400 parts of crataegus pinnatifida, 100-250 parts of prunella vulgaris, 100-250 parts of rhodiola rosea, 30-70 parts of leech, 200-400 parts of codonopsis pilosula, 30-70 parts of ganoderma lucidum, 30-70 parts of ginseng or American ginseng, and 80-150 parts of gynostemma pentaphylla. The preparation method comprises the steps of weighing raw materials; preparing powder from each raw material; mixing all raw materials to obtain a first mixture; preparing pills from the first mixture; baking the pills; packaging. The hypotensive drug can play a good blood pressure reduction effect, is small in side effect, and has the effects of calming and preventing angiosclerosis.

The invention relates to the technical field of blood purification, and discloses a coated heparin-immobilized hemoperfusion adsorbent and a preparation method thereof. The preparation method includesthe steps: soaking a hemoperfusion adsorbent in an aqueous solution of dopamine hydrochloride and heparin salt, and performing slow stirring at room temperature; and adjusting the pH value to be alkaline, continuously stirring at room temperature to realize coating of the polydopamine on the hemoperfusion adsorbent, and immobilizing the heparin on the adsorbent. The polydopamine generated in theinvention has strong adhesion and can be firmly coated on the hemoperfusion adsorbent to realize coating, the blood compatibility of the hemoperfusion adsorbent is improved, and the immobilized heparin has excellent anticoagulation performance and can reduce the blood coagulation phenomenon when blood is in contact with the adsorbent. The preparation method is simple and effective, is suitable forvarious hemoperfusion products clinically used at present, and improves the safety of clinical application.

The invention discloses a biological material with functions of catalytic releasing NO and capturing EPCs and a preparation method thereof, and relates to the technical field of medical apparatus andinstruments. The preparation method includes steps of depositing a metal oxide film on the surface of a material to be modified, immersing the material in an azide o-phenol compound solution, and thenimmersing the material in a functionalized modification solution, specifically, the functionalized modification solution contains alkyne-based biomolecules capable of catalytic releasing NO and alkyne-based biomolecules with function of capturing EPCs. The prepared biological material comprises a substrate material, wherein the substrate material is sequentially deposited with a metallic oxide, the azide o-phenol compound and the alkyne-based functional biomolecules, and the functional biomolecules contain the biomolecules capable of catalytic releasing NO and the biomolecules with function of capturing EPCs. The material has the function of catalytic releasing NO and the function of capturing EPCs as well, so that the blood anticoagulation property of the material is improved, the smoothmuscle hyperplasia is inhibited, and the stent-in-situ re-endothelialization is promoted.

The invention discloses a sulfonated citric acid chitosan modified polysulfone hemodialysis film and a preparation method thereof. Sulfonated citric acid chitosan modified polysulfone (SCACS-PSF) is used as a film material, the mass percentage content of a film solution is 15-25%, the mass percentage content of a solvent is 75-85%, and the sulfonated citric acid chitosan modified polysulfone hemodialysis film with good biocompatibility is prepared by a dry-wet spinning method. The asymmetric hemodialysis film prepared by the invention has a hollow fiber structure; the inner and outer surfacesare compact skin layers; the middle supporting layer is porous; the hemodialysis film has an inner diameter of 130-260 microns, a wall thickness of 20-60 [mu] m, and an ultrafiltration coefficient of5 to 55 mL/m < 2 >. H.mmHg; the hemodialysis film is a film module which has high permeability and high separability; the hemodialysis film has good anticoagulation and antibacterial properties, the removal rates of urea, beta-microglobulin and albumin are 58%-85%, 50%-62% and 3.0%-9.8% respectively, the removal rate of pathogenic escherichia coli is 99%, and the removal rate of staphylococcus aureus is 99.5%-100%.

The invention provides a method for preparing a multilayer heparin-loaded reduced graphene oxide coating, comprising the following steps: S1, preparing a polylysine coating on the surface of a biological material; S2, preparing a heparin-loaded reduced graphene oxide solution ; S3, preparing a single-layer heparin-loaded reduced graphene oxide coating; S4, alternately immersing the biological material prepared in step S3 into a polylysine solution and a heparin-loaded reduced graphene oxide solution, and sequentially adsorbing respectively to obtain Multilayer heparin-loaded reduced graphene oxide coating. The beneficial effect of the invention is that the anticoagulant performance of the surface of the material can be significantly improved.