119results about How to "Improve anticoagulant performance" patented technology

The invention relates to a highly ticoagulant cellulose membrane material, wherein an amphichroic ionomer layer is grafted on the surface of a cellulose membrane, and a 'brush'-shaped amphichroic ionomer layer is formed on the surface of the cellulose membrane. The cellulose membrane with good biocompatibility is prepared by grafting of amphichroic ionic molecules on the surface of the material. The neat 'brush'-shaped amphichroic ionomer layer is formed on the surface of cellulose, so that the defects on the surface of the material are overcome, and the action between the material and biomacromolecules is further reduced, thereby nonspecific protein absorption and blood platelet adhesion are reduced to the maximum degree. Therefore, the cellulose membrane material has high nonspecific protein absorption resistance and high ticoagulant performance. The invention discloses a method for manufacturing the highly ticoagulant cellulose membrane material.

The invention discloses a method for constructing an anti-blood coagulation blood vessel scaffold material by utilizing an endogenous NO donor. The method comprises the steps that organic selenium polycation high polymer and polyanion high polymer are alternately coated on the surface of the blood vessel scaffold material in sequence through a layer-by-layer self-assembled method to form a blood vessel scaffold material surface decorated by a multilayer bimolecular film, thus catalyzing the endogenous NO carries such as S-nitroso glutathione (GSNO) existing in the body, realizing sustained and partial release of NO, effectively regulating and controlling the release rate of NO by regulating and controlling the number of layers of the layer-by-layer self-assembled bimolecular film, and further improving blood coagulation resistance of the blood vessel scaffold material. The method of the invention can be used for metal blood vessel scaffolds or organic polymer artificial blood vessel scaffolds or cell-free artificial blood vessel scaffolds, and also can be used for other medical appliances contacting with blood.

A Chitosan-arginine conjugate as anticoagulant material is prepared through dissolving chitosan in the solution of N, N, N'-tetramethyl ethanediamine, adding 1-ethyl-3-(3-dimethylamino propyl) carbodiimine, N-hydroxy-butanediimide and arginine, reacting while magnetic stirring, dialyzing, drying to obtain said conjugate, adding glucosyl aldehyde oxide, and cross-linking reaction to become film. Its advantages are high effect and low poison to cell.

The invention takes diazo-resin or multivalence metal ion solution as cross-linking agent. Through the electrostatic attraction LBL self-assembly technology, overstable endovascular stent graft anti-coagulation coating is prepared to lead the anti-coagulation coating to have good blood compatibility and tissue compatibility. The invention is realized in the following steps: a, the surface of an endovascular stent graft is cleaned, firstly, the surface of the endovascular stent graft is ultrasonically cleaned with multiple organic solvents for 1 to 120 minutes, and then the surface thereof is rinsed to be clean with water; b, oxidation treatment: H2O2 is boiled for 1 to 120 minutes, and then is rinsed with fresh water, KOH solution is etched for 1 to 120 minutes and then flushed to be clean with a large amount of fresh water; c, the preparation of the anti-coagulation coating: the diazo-resin with positive electricity and a polymer with anti-coagulation reactivity and / or the other polyanion solution are alternately deposited on the surface of the material through the electrostatic attraction function, ultraviolet light or visible light is adopted to irradiate surface coating of the material, through the photochemical reaction, ionic linkage between the inner layers of the coating is converted into covalent linkage, so as to attain the overstable anti-coagulation coating; or multivalence metal positive ion and the polymer or polyanion with anti-coagulation reactivity are alternately deposited on the surface of the stent graft material through the electrostatic attraction function, so as to attain the stable anti-coagulation coating.

The invention relates to the field of tissue engineering, in particular to a preparation method of a composite nanometer fiber small-diameter intravascular tissue engineering stent material. The technical scheme is achieved as follows: formic acid is used as a common solvent; RGD-recombinant spider silk protein, polycaprolactone and chitosan are mixed in a mass ratio of 1:8:1 according to the requirements of an intravascular stent to prepare a spinning solution with the concentration of 20-30 percent (w / v); electrospinning process parameters are as follows: the diameter of a cross section of acylindrical rotating shaft is 3-6 mm, the rotating speed is 1,500-3,000rpm, the curing distance is 15-30 cm, the voltage is 50-150kV, the extrusion speed is 3-7ml / h and the temperature is 45-50 DEG C. The small-diameter intravascular stent has favorable cell adhesion-promoting capability, anticoagulant property and mechanical property for resisting physiological environment and can be clinicallyapplied as a small-diameter intravascular tissue engineering stent.

The invention discloses a shape memory type high-elasticity activity nano-fiber stent and application thereof. The shape memory type high-elasticity activity nano-fiber stent is manufactured by introducing biological activity components (laminin, heparin, CD34+, VEGF and the like) into degradable linear elasticity polyester PCT with a shape memory effect through an electrostatic spinning technology; the molecular weight of the linear elasticity polyester PCT is 50000-400000, and the percentage composition of functionality caprolactone monomers, containing side cyclic ether substitutes, in a copolyester is 5-50%. The shape memory type high-elasticity activity nano-fiber stent is a high-activity nano-fiber stent with human body biomechanics elasticity, is a three-dimensional degradable tissue engineering stent which can provide an ideal biomechanics environment and an ideal biological activity microenvironment for cell growth, and is expected to be widely applied to regeneration and repair of elastic human body tissue such as blood vessels, cardiac muscles, nerves, skin, tendon, heart and the like.

The invention discloses an anticoagulation coating material and a preparation method thereof. The anticoagulation coating material comprises sodium hyaluronate and hirudin, wherein the sodium hyaluronate is formed into a gel network; the hirudin is in covalent binding with the sodium hyaluronate. The preparation method of the anticoagulation coating material comprises the following steps: (1) preparing an MES (Fatty Acid Methyl Ester Sulfonate) buffer solution; (2) preparing a sodium hyaluronate solution with the concentration of 1mg / ml to 20mg / ml; (3) preparing a hirudin solution with the concentration of 1mg / ml to 10mg / ml; (4) uniformly mixing up the two solutions so as to enable the mass ratio of the hirudin to the sodium hyaluronate to be 1:1 to 1:10; (5) adding a crosslinking agent into the mixture solution obtained in the step (4), uniformly mixing up so as to covalently crosslink the hirudin and the sodium hyaluronate. The anticoagulation coating material disclosed by the invention is high in anticoagulation activity, stable in effect, long in lasting time, good in biocompatibility, simple in preparation method, gentle in condition and applicable to large-scale industrial production.

The invention relates to a small-caliber artificial blood vessel with the surface grafted with a heparin coating and a preparation method of the small-caliber artificial blood vessel. A small-caliberblood vessel is adopted as a substrate. The preparation method comprises the following steps: soaking the surface of the substrate in a dopamine solution for reaction, coating the surface of the substrate with multiple layers of polydopamine coatings, performing chemical bond combination grafting with polyethyleneimine, and finally activating heparin by utilizing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) to obtain the heparinized surface through amido bond combination. The invention has the characteristics of small caliber, favorable mechanical properties, hydrophilicity and long-acting anticoagulation; the preparation method is environmentally friendly and efficient in compounding, the prepared heparin coating is uniform, the grafting amount of heparin is higher and can reach 4.9 mu g / cm < 2 >, and good market application prospects are achieved.

Preparation technology for a bionic ceramic / metal laminating composite support coating includes steps of a, performing double-cathode plasma sputtering deposition for a Ta layer to obtain a Ta layer with the thickness of 100-200nm at first; b, performing ion oxidation for 5-10min; and c, performing double-cathode plasma sputtering decomposition for an Au or Mg layer to obtain an Au or Mg layer with the thickness of 10-20nm, and repeating the steps a, b and c to obtain a laminating coating. Sputtered targets include targets made of one of Au and Mg and pure-Ta targets; and materials of workpieces include 316L stainless steel. The Ta2O5 layer in the coating has good corrosion resistance and blood compatibility, the metal Au or Mg layer has low elastic modulus and good biocompatibility, toughness of the coating can be increased, and furthermore, bonding strength of the coating and a base material can be improved.

The present invention belongs to the field of anticoagulant material technology. The anticoagulant surface modification process of artificial implant includes the following steps: cleaning artificial implant; preparing silicone rubber solution; coating the artificial implant with medical silicone rubber as support; coating perfluoro sulfonic acid on the surface of the silicone rubber coating; depositing polycation and polyanion alternately onto the surface of the perfluoro sulfonic acid coating via electrostatic attraction; and irradiating the artificial implant with ultraviolet light to produce photochemical reaction to convert the ionic bond between coating layers into covalent bond. The present invention has simple technological process, mild reaction condition, easy operation, high repeatability, environment friendship and thus excellent application foreground.

The invention provides a drug-loading intravascular stent which comprises a stent body and a drug coating located on the stent, wherein the outer surface of stent coating is arranged as a smooth mirror surface; the coating comprises a first coating, a second coating and a third coating in turn from the surface of the stent body to the outer side; dopamine is used as a coating carrier of the first coating and is grafted with a substance A; PLGA is used as the coating carrier of the second coating; the second coating comprises a substance B; PLGA is used as the coating carrier of the third coating; the third coating comprises drugs C and B; A is heparin, B is sirolimus and C is curcumin. The invention also provides the preparation method for the stent. The method comprises the following steps: treating the stent; preparing a freeze-drying material; preparing the first coating; preparing the second coating; preparing the third coating. The invention has the advantages that three-layer drug loading coatings are arranged, the drugs are released layer by layer in the sequence of sirolimus, curcumin and heparin after the stent is implanted, the sequence is conformed to the occurrence trend of thrombus and smooth muscle proliferation, the effective drug duration is prolonged and the inhibiting effect for the subsequent potential thrombus and smooth muscle proliferation is achieved.

The invention provides a coating method for an activated carbon adsorbent for blood perfusion. Firstly, coating film is formed on the surface of activated carbon with chitosan, then crosslinking is executed with epichlorohydrin to increase the strength of the film, and finally heparin sodium having an anticoagulant effect is grafted to the surface of the film. The crosslinked chitosan film can improve the blood compatibility of the activated carbon and decrease particle falling of the activated carbon; the heparin sodium having an excellent anticoagulant property is grafted on the film so thatthe blood coagulation phenomenon occurring when blood is in contact with the surface of the activated carbon can be decreased, and the safety of a perfusion device is improved.

The invention relates to a method for preparing rutile-type TiO2 thin film at room temperature, belonging to the field of surface engineering technique and biological materials. The single rutile-type TiO2 thin film is obtained on the surface of superfine crystal / nanometer crystal titanium at the room temperature by performing nanorization treatment on a titanium microstructure in advance and adopting a direct-current reaction magnetic control sputtering deposition technique. After nanorization of the titanium base material microstructure, the interface bonding force between the TiO2 thin film and the base material is improved remarkably, the technical problem of poor bonding condition between the TiO2 thin film with excellent anticoagulant property and a titanium vessel bracket is bettersolved and the bearing capacity of the interface is greatly improved. The invention better solves the problem that the single rutile-type TiO2 thin film is hard to prepare on the titanium surface at the room temperature, has simple process and is widely applied to the fields of vessel brackets, heart valves, and the like.

The invention belongs to the field of biological materials, and particularly relates to an anticoagulant composite hollow fiber membrane and a preparation method thereof. The fiber membrane comprisespolyethersulfone, maleylated chitosan and a water-soluble polymer pore-forming agent, wherein the polyethersulfone is in a porous grid framework structure, and after being modified by the maleylated chitosan, the biocompatibility of the polyethersulfone is increased, the platelet aggregation is reduced, and the anticoagulant performance is improved; by adding the water-soluble polymer pore-formingagent, on one hand, the inner diameter, pore diameter and porosity of the fiber membrane can be adjusted, so that the permeability of the fiber membrane is controlled; on the other hand, the water-soluble polymer pore-forming agent is embedded in the porous grid framework structure and is matched with the maleylated chitosan, so that the stability is good, the water-soluble polymer pore-forming agent is not easy to elute and can be used for a long time. The fiber membrane is used as a dialysis membrane to carry out hemodialysis on a patient without using heparin, so that the diseases such asosteoporosis, low coagulation performance and the like of the patient are avoided. The preparation method is simple and suitable for industrial production.

Belonging to the field of biomedical engineering, the invention specifically related to a silk artificial blood vessel and a preparation method thereof. The artificial blood vessel includes a silk woven blood vessel body and glycosylated cross-linking sericin filling blood vessel body fiber gaps. The preparation method includes the steps of: preparation of the blood vessel body, compounding of the blood vessel body and glycosylated sericin, blood vessel surface modification and endothelial pre-coating. In the invention, glycosylated sericin is compounded with silk, then aldehyde is employed to crosslink sericin so as to reduce water solubility, the gaps between artificial blood vessel silk fiber are filled to prevent blood leakage, and the rigidity of the blood vessel is improved. The glycosylated sericin is located on the artificial blood vessel surface, can reduce immunogenicity and improve the anticoagulation effect, and makes the artificial blood vessel difficult to degrade, thus overcoming the two defects of easy degradation and strong immunogenicity in the prior art. And the silk artificial blood vessel has the advantages of simplicity and practicability, good quality and low price.

The invention discloses a lysine polyurethane medical material with the surface modified by carboxymethyl chitosan. A base layer is formed with a medical polyurethane material or a synthesized polyurethane material, and a modification layer is formed on the surface of the base layer through a chemical modification method. The modification layer contains carboxymethyl chitosan linked with a polyurethane substrate material through a covalent bond and lysine linked with carboxymethyl chitosan through a covalent bond by means of an active group on carboxymethyl chitosan. The invention further discloses a preparing method of the lysine polyurethane medical material with the surface modified by carboxymethyl chitosan. Reactivity points are generated on the surface of polyurethane through pre-activating treatment, then polyurethane and carboxymethyl chitosan are grafted and copolymerized, and the hydrophilia and biocompatibility of polyurethane as the medical material are improved; by introducing a second modification layer of lysine to the system, the anticoagulation property of polyurethane as the medical material is improved, production is simple, cost is low, and industrial popularization is easy.

The invention discloses a micro-nano multi-scale patterned anticoagulation composite biological material and a method for preparing the same. The micro-nano multi-scale patterned anticoagulation composite biological material comprises a titanium dioxide (TiO2) nano-wire array and an amorphous carbon film wrapping the top end of the nano-wire array. The method for preparing the anticoagulation composite biological material includes the steps: firstly, arranging micron-sized patterns on a fluorine-doped tin oxide (FTO) conductive glass substrate through the mask plate photoetching technology, secondly, enabling the TiO2 nano-wire array to grow on the patterned substrate by means of hydro-thermal synthesis, and enabling the TiO2 nano-wire array to grow on a micron-sized patterned area, so that the whole TiO2 nano-wire array is in a micro-nano patterned structure, and finally, wrapping the amorphous carbon film on the prepared micro-nano patterned array. By the aid of composition of two materials and the design of the micro-nano patterned structure, properties such as surface appearance, hydrophilicity and hydrophobicity, electron transfer characteristic and the like of the materials can be changed and controlled, and blood compatibility of the composite material is improved.

The invention relates to the field of medical devices, in particular to a preparation method of a surface-heparinized anticoagulation medical device based on ionic bond-covalent bond synergistic action. The method comprises the following steps: firstly, fixing heparin on the surface of a medical device by utilizing a hydrophilic polymer modified by low-molecular-weight hyperbranched polyethyleneimine as a side chain in an ionic bond combination manner; and furthermore, grafting a stable heparin coating through covalent bonds, so as to obtain the anticoagulant biomedical device with high anticoagulant activity and high anticoagulant stability. The device has a wide application value in the field of biomedical treatment. The method is simple in preparation process, high in controllability and low in material price, does not involve toxic reagents in the experiment process, has universality for various medical device structures, and is suitable for industrial production.

The invention discloses modified polycarbonate with high anticoagulant activity. Modified polycarbonate is a linear macromolecular polymer, wherein a side chain of the polymer contains functionalizeddouble bonds and hydrophilic phosphorylcholine groups. Modified polycarbonate comprises copolymers A, B and C. The preparation process of modified polycarbonate comprises the following steps: copolymerizing TMC (trimethylene carbonate) and a trimethylene derivative firstly, and then grafting the side chain of the copolymer with MPC (2-methacryloyloxyethyl phosphorylcholine) through a chick reaction of dithiol and the double bonds to obtain the target product modified polycarbonate with high anticoagulant activity. According to modified polycarbonate with high anticoagulant activity, performance of polycarbonate can be changed by adjusting monomer types and proportion and quantity of the phosphorylcholine groups on the side chain, and the anticoagulant capability of polycarbonate as a biodegradable medical polymer material can be improved; reaction sites can be provided by the double bonds on the side chain for follow-up grafting with biomacromolecules or other functional molecules, theresearch potential is high, and the derivative performance is wide.

The invention belongs to the field of biological materials and discloses a surface-heparinized cellulose ester liquid crystal material and a preparation method and application thereof. The preparation method of the liquid crystal material comprises the following steps of: (1) preparation of acryloyl-octanoyl-hydroxy propyl cellulose ester liquid crystal: dissolving hydroxy propyl cellulose into an acetone solution, adding acryloyl chloride and reacting; after the reaction is over, cleaning the obtained mixed solution for 3-5 times, and dialyzing and drying; dissolving the obtained product in acetone, dropwise adding octanoyl chloride and reacting; and cleaning, dialyzing and drying to obtain acryloyl-octanoyl-hydroxy propyl cellulose ester; and (2) preparation of surface-heparinized cellulose ester liquid crystal material: dissolving the acryloyl-octanoyl-hydroxy propyl cellulose ester liquid crystal in tetrahydrofuran, fetching the solution to form a film, then adding a sodium hydroxide solution of heparin sodium, reacting and cleaning to obtain the surface-heparinized cellulose ester liquid crystal material.

The invention relates to a biodegradable polymer containing phosphorylcholine (PC) and polyethylene glycol (PEG) and a synthetic method thereof. The synthetic method comprises: MPC with the mass ratio of 1-99% and PEG-PLA which is connected with double linkage and has the mass ratio of 99-1% are dissolved in trichloromethane and then added with free radical polymerization initiator to react for 6-24h at the temperature of 0-80 DEG C, and the products are precipitated by methyl alcohol and dried in vacuum; the PEG-PLA which is connected with double linkage is prepared by ring opening polymerization of lactide initiated by the PEG that reacts with acryloyl chloride at a single end. The phosphorylcholine group which has positive and negative charges and is introduced on the PLA of the polymer can be seen according to the static contact angle result, and then the contact angle is obviously reduced, so that hydrophilicity is greatly improved. The higher the MPC content is, the smaller the contact angle is, and the better the wettability of the material is. Test results of anticoagulation prove that the PEG can reduce coagulation of blood platelet, affects prothrombin time (PT) and effectively avoids the activation of an extrinsic coagulation system; the high content PC group can effectively reduce the conglutination of the blood platelet and has influence on anginal partial thromboplastin time (APTT) of the activation part.

The invention provides a hypotensive drug and a preparation method thereof. The hypotensive drug comprises the following components in parts by weight: 200-400 parts of kudzuvine root, 200-400 parts of red sage root, 80-150 parts of ligusticum wallichii, 200-400 parts of astragalus, 100-250 parts of cassia seed, 200-400 parts of crataegus pinnatifida, 100-250 parts of prunella vulgaris, 100-250 parts of rhodiola rosea, 30-70 parts of leech, 200-400 parts of codonopsis pilosula, 30-70 parts of ganoderma lucidum, 30-70 parts of ginseng or American ginseng, and 80-150 parts of gynostemma pentaphylla. The preparation method comprises the steps of weighing raw materials; preparing powder from each raw material; mixing all raw materials to obtain a first mixture; preparing pills from the first mixture; baking the pills; packaging. The hypotensive drug can play a good blood pressure reduction effect, is small in side effect, and has the effects of calming and preventing angiosclerosis.

The invention discloses an Nd2O3-containing gradient bioactive ceramic coating material and a preparation method thereof. The coating material comprises the following raw materials in parts by weight: 0 to 70 parts of titanium powder and 30 to 100 parts of compound ceramic powder, wherein the compound ceramic powder is prepared through the steps of mixing 72 to 80 parts of CaHPO4.2H2O with 28 to 20 parts of CaCO3 so as to obtain 100 parts of ceramic powder, and then adding 0.1 to 0.8 parts of Nd2O3 powder into the obtained ceramic powder. In the invention, because of adding the Nd2O3 powder into the ceramic powder, the ceramic coating has the function of catalyzing the synthesis of hydroxyapatite and beta-calcium phosphate, and compared with the actions of the ceramic powder added with CeO2, La2O3 and Y2O3 powder, a biological ceramic coating prepared by adding Nd2O3 powder has better biocompatibility and biological activity, has the function of anticoagulation and can prevent canceration; meanwhile, compared with the action of the ceramic powder added with CeO2, La2O3 and Y2O3 powder, more micro-pores are formed on the surface of the biological ceramic coating prepared through adding Nd2O3 powder, thereby providing more channels for a bone tissue ingrowth coating.

The invention discloses an acrylic acid and sulfonated dihydroxypropyl chitosan modified polysulfone membrane and a preparation method thereof. The preparation method comprises the following steps: grafting acrylic acid (AA) on the surface of a polysulfone membrane (PSF membrane) through ultraviolet grafting to obtain an acrylic acid modified polysulfone membrane (AA-PSF membrane); grafting sulfonated dihydroxypropyl chitosan (SDHPCS) on the surface of the AA-PSF membrane through a condensation reaction of carboxyl and amino to obtain the acrylic acid and sulfonated dihydroxypropyl chitosan modified polysulfone membrane (SDHPCS-AA-PSF membrane). The invention is characterized by adopting the polysulfone membrane as a base membrane, grafting the acrylic acid on the membrane surface and thengrafting the sulfonated dihydroxypropyl chitosan to obtain the acrylic acid and sulfonated dihydroxypropyl chitosan modified polysulfone membrane which has good hydrophilcity and blood anticoagulation; moreover, the preparation technology is simple, and the conditions are mild and easy to control.

The invention relates to the technical field of blood purification, and discloses a coated heparin-immobilized hemoperfusion adsorbent and a preparation method thereof. The preparation method includesthe steps: soaking a hemoperfusion adsorbent in an aqueous solution of dopamine hydrochloride and heparin salt, and performing slow stirring at room temperature; and adjusting the pH value to be alkaline, continuously stirring at room temperature to realize coating of the polydopamine on the hemoperfusion adsorbent, and immobilizing the heparin on the adsorbent. The polydopamine generated in theinvention has strong adhesion and can be firmly coated on the hemoperfusion adsorbent to realize coating, the blood compatibility of the hemoperfusion adsorbent is improved, and the immobilized heparin has excellent anticoagulation performance and can reduce the blood coagulation phenomenon when blood is in contact with the adsorbent. The preparation method is simple and effective, is suitable forvarious hemoperfusion products clinically used at present, and improves the safety of clinical application.

The invention discloses a biological material with functions of catalytic releasing NO and capturing EPCs and a preparation method thereof, and relates to the technical field of medical apparatus andinstruments. The preparation method includes steps of depositing a metal oxide film on the surface of a material to be modified, immersing the material in an azide o-phenol compound solution, and thenimmersing the material in a functionalized modification solution, specifically, the functionalized modification solution contains alkyne-based biomolecules capable of catalytic releasing NO and alkyne-based biomolecules with function of capturing EPCs. The prepared biological material comprises a substrate material, wherein the substrate material is sequentially deposited with a metallic oxide, the azide o-phenol compound and the alkyne-based functional biomolecules, and the functional biomolecules contain the biomolecules capable of catalytic releasing NO and the biomolecules with function of capturing EPCs. The material has the function of catalytic releasing NO and the function of capturing EPCs as well, so that the blood anticoagulation property of the material is improved, the smoothmuscle hyperplasia is inhibited, and the stent-in-situ re-endothelialization is promoted.

The invention discloses a polyacrylonitrile-carboxymethyl chitosan composite hemodialysis membrane, which is prepared from the following components in parts by weight: 35 to 50 parts of polyacrylonitrile, 20 to 30 parts of carboxymethyl chitosan, 10 to 18 parts of polyacrylate, 0.005 to 0.02 part of heparin, 32 to 60 parts of solvent, and 15 to 50 parts of water. The invention also discloses a preparation method of the polyacrylonitrile-carboxymethyl chitosan composite hemodialysis membrane. Hydrophilic biomass carboxymethyl chitosan and anticoagulant heparin are introduced into the polyacrylonitrile through blended spinning, so that the hydrophilia and the biocompatibility of the polyacrylonitrile used as the hemodialysis membrane are improved, and meanwhile, a certain anticoagulation function is realized; the method is simple and practicable, rich in raw material source, low in price, and easy to popularize.

The invention discloses a sulfonated citric acid chitosan modified polysulfone hemodialysis film and a preparation method thereof. Sulfonated citric acid chitosan modified polysulfone (SCACS-PSF) is used as a film material, the mass percentage content of a film solution is 15-25%, the mass percentage content of a solvent is 75-85%, and the sulfonated citric acid chitosan modified polysulfone hemodialysis film with good biocompatibility is prepared by a dry-wet spinning method. The asymmetric hemodialysis film prepared by the invention has a hollow fiber structure; the inner and outer surfacesare compact skin layers; the middle supporting layer is porous; the hemodialysis film has an inner diameter of 130-260 microns, a wall thickness of 20-60 [mu] m, and an ultrafiltration coefficient of5 to 55 mL / m < 2 >. H.mmHg; the hemodialysis film is a film module which has high permeability and high separability; the hemodialysis film has good anticoagulation and antibacterial properties, the removal rates of urea, beta-microglobulin and albumin are 58%-85%, 50%-62% and 3.0%-9.8% respectively, the removal rate of pathogenic escherichia coli is 99%, and the removal rate of staphylococcus aureus is 99.5%-100%.

The invention provides an anticoagulant antimicroorganism coating and a preparation method and use thereof. The anticoagulant antimicroorganism coating based on dopamine-assisted codeposition has a structure as follows: a side close to a base material is taken as an inner side, a dopamine coating and an anticoagulant antimicroorganism composite layer are sequentially arranged from inside to outside and the dopamine coating and the anticoagulant antimicroorganism composite layer are coated on a surface of the base material; wherein the dopamine coating takes dopamine or levodopa and polyethyleneimine as raw materials, and the anticoagulation antimicroorganism composite layer takes an anticoagulation material, an antimicroorganism material, EDC(1-(3-dimethylaminopropyl)-3-ethylcarbodiimide) and NHS(N-hydroxysuccinimide) as raw materials; and hydroxyls and quinonyls of the dopamine coating are covalently bound with aminos and carboxyls of the anticoagulant antimicroorganism composite layer to realize chemical compounding of the two layers.

The invention provides a method for preparing a multilayer heparin-loaded reduced graphene oxide coating, comprising the following steps: S1, preparing a polylysine coating on the surface of a biological material; S2, preparing a heparin-loaded reduced graphene oxide solution ; S3, preparing a single-layer heparin-loaded reduced graphene oxide coating; S4, alternately immersing the biological material prepared in step S3 into a polylysine solution and a heparin-loaded reduced graphene oxide solution, and sequentially adsorbing respectively to obtain Multilayer heparin-loaded reduced graphene oxide coating. The beneficial effect of the invention is that the anticoagulant performance of the surface of the material can be significantly improved.