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Method for preparing multilayer heparin-loaded reduced graphene oxide coating

A graphene and coating technology, which is applied in the field of preparing multi-layer heparin-loaded reduced graphene oxide coatings, can solve the problem of poor anticoagulation effect for a long time, easy destruction of heparin anticoagulant biological activity, and weak heparin binding on the surface, etc. problems, to achieve the effect of improving anticoagulant performance, wide application, and promoting cell growth

Inactive Publication Date: 2017-06-09
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The covalent immobilization method uses various means (such as plasma treatment, surface self-assembly, ozonation, etc.) to introduce reactive groups on the surface of the material, and uses the reaction of the group with the carboxyl or hydroxyl group in heparin to introduce heparin onto the surface of the material. , but the anticoagulant biological activity of heparin is easily destroyed due to chemical reaction
[0006] The ion binding method, also known as the electrostatic adsorption method, uses various methods to treat the surface of the material to introduce positive charges, so that negatively charged heparin can be adsorbed. However, this method has weak surface heparin binding and a small amount of heparin. The coagulation effect, especially the long-term anticoagulant effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: according to the following steps, the surface of the material is treated

[0028] (1) Prepare a PLL coating on the surface of the biological material, first immerse the cleaned and dried sample in a 1 mg / ml dopamine hydrochloride solution, adjust the pH value to 8-8.5 with NaOH, and react with distilled water after 24 hours at room temperature After fully cleaning and drying, repeat this step three times to obtain a polydopamine coating, and then immerse the prepared material in a 5mg / ml polylysine solution to react for 4 hours. After the sample is taken out, it is fully washed and dried with distilled water to obtain a PLL coating.

[0029] (2) To prepare heparin-loaded reduced graphene oxide solution, first configure 0.1mg / ml graphene oxide solution, take 20ml graphene oxide solution and add 0.2mg hydrazine monohydrate, fully stir and react at 80°C for 2 hours, centrifuge and dry to obtain reduced Graphene oxide, re-dispersed into a solution of 0.1 mg / m...

Embodiment 2

[0032] Embodiment 2: according to the following steps, the surface of the material is processed

[0033] (1) Prepare a PLL coating on the surface of the biological material, first immerse the cleaned and dried sample in a 1 mg / ml dopamine hydrochloride solution, adjust the pH value to 8-8.5 with NaOH, react at room temperature for 12 hours, and then use distilled water After fully cleaning and drying, repeat this step three times to obtain a polydopamine coating, and then immerse the prepared material in an 8mg / ml polylysine solution to react for 24 hours. After the sample is taken out, it is fully washed and dried with distilled water to obtain a PLL coating.

[0034] (2) To prepare heparin-loaded reduced graphene oxide solution, first configure 0.1mg / ml graphene oxide solution, take 20ml graphene oxide solution and add 0.6mg sodium borohydride, fully stir and react at 120°C for 8 hours, centrifuge and dry to obtain the reduced graphene oxide solution Graphene oxide, re-disp...

Embodiment 3

[0037]Embodiment 3: according to the following steps, the material surface is processed

[0038] (1) Prepare a PLL coating on the surface of the biological material, first immerse the cleaned and dried sample in a 1 mg / ml dopamine hydrochloride solution, adjust the pH value to 8-8.5 with NaOH, and react with distilled water after 24 hours at room temperature After fully cleaning and drying, repeat this step three times to obtain a polydopamine coating, and then immerse the prepared material in a 10mg / ml polylysine solution to react for 12 hours. After the sample is taken out, it is fully washed and dried with distilled water to obtain a PLL coating.

[0039] (2) To prepare a heparin-loaded reduced graphene oxide solution, first prepare a 0.1 mg / ml graphene oxide solution, take 20 ml of graphene oxide solution and add 1 mg of sodium borohydride, fully stir and react at 120°C for 8 hours, and centrifuge and dry to obtain the reduced graphene oxide solution Graphene was re-dispe...

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Abstract

The invention provides a method for preparing a multilayer heparin-loaded reduced graphene oxide coating, comprising the following steps: S1, preparing a polylysine coating on the surface of a biological material; S2, preparing a heparin-loaded reduced graphene oxide solution ; S3, preparing a single-layer heparin-loaded reduced graphene oxide coating; S4, alternately immersing the biological material prepared in step S3 into a polylysine solution and a heparin-loaded reduced graphene oxide solution, and sequentially adsorbing respectively to obtain Multilayer heparin-loaded reduced graphene oxide coating. The beneficial effect of the invention is that the anticoagulant performance of the surface of the material can be significantly improved.

Description

technical field [0001] The invention relates to a method for preparing a graphene oxide coating, in particular to a method for preparing a multilayer heparin-loaded reduced graphene oxide coating. Background technique [0002] Biomaterials and implantable medical devices have important applications in improving human health and preventing diseases. However, for blood contact materials and intravascular implanted medical devices, the coagulation reaction caused by the interaction between materials and blood may It will cause a series of side effects such as thrombosis and clinical complications, and eventually lead to implant failure. Therefore, the surface properties of materials often play a decisive role in the success of implantation. Surface modification is a key technology to improve the blood compatibility of materials. . [0003] Heparin is a kind of glucosamine with excellent anticoagulant properties. It has excellent anticoagulant effect both in vivo and in vitro. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/08A61L33/00
CPCA61L31/10A61L31/084A61L33/0011A61L2420/02A61L2420/06A61L2420/08
Inventor 刘乃山宋延超迟培升
Owner QINGDAO JIULONG BIO PHARMA
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