Titanium alloy surface collagen coating loaded with antibacterial polypeptides and preparation method of coating

A technology of antibacterial peptides and titanium alloys, which can be applied to devices, coatings, and pharmaceutical formulations for coating liquid on the surface, and can solve the problems of poor bonding between the surface of titanium alloys and collagen coatings, physical pain and economic burden of patients, tissues and organs Necrosis and other problems, to achieve high biological activity, reduce the risk of infection, good antibacterial effect

Active Publication Date: 2019-10-01
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the surface of the titanium alloy is poorly bonded to the collagen coating, and the coating is easy to fall off
[0004] In addition, the surface of titanium alloy implant materials is easy to accumulate bacterial plaque, and the host immunity on the surface of the implant is poor, which makes it easy to cause bacterial infection after the titanium alloy enters the human body, and the adhesion of bacteria and the formation of biofilm on the surface of the implant material can easily lead to infection. The infection caused by the material interface leads to the loosening and falling off of the implanted material, and even causes the necrosis of adjacent tissues and organs, which brings greater pain and economic burden to the patient's body. Therefore, it is necessary to improve the antibacterial properties of the titanium alloy surface. Improving the implant effect of titanium alloy

Method used

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  • Titanium alloy surface collagen coating loaded with antibacterial polypeptides and preparation method of coating
  • Titanium alloy surface collagen coating loaded with antibacterial polypeptides and preparation method of coating
  • Titanium alloy surface collagen coating loaded with antibacterial polypeptides and preparation method of coating

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Embodiment 1

[0034] As an embodiment of the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides of the present invention, the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides described in this embodiment comprises the following steps:

[0035] (1) Sample pretreatment: Use acetone, ethanol, and pure water to ultrasonically clean the micro-arc oxidation porous titanium alloy in sequence, wash each reagent three times, each time for 10 minutes, and dry in a vacuum oven after cleaning;

[0036] (2) dissolving collagen in hexafluoroisopropanol, stirring magnetically at room temperature for 3 hours, and preparing a collagen solution with a concentration of 3 wt %;

[0037] (3) Add a dopamine solution with a concentration of 4 mg / mL to the collagen solution obtained in step (1), wherein the weight ratio of dopamine to collagen is 0.15:1, and after reacting at room temperature ...

Embodiment 2

[0040] As an embodiment of the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides of the present invention, the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides described in this embodiment comprises the following steps:

[0041] (1) Sample pretreatment: Use acetone, ethanol, and pure water to ultrasonically clean the micro-arc oxidation porous titanium alloy in sequence, wash each reagent three times, each time for 10 minutes, and dry in a vacuum oven after cleaning;

[0042] (2) dissolving collagen in hexafluoroisopropanol, stirring magnetically for 3 hours at room temperature, and preparing a collagen solution with a concentration of 6 wt %;

[0043] (3) Add a dopamine solution with a concentration of 1 mg / mL to the collagen solution obtained in step (1), wherein the weight ratio of dopamine to collagen is 0.3:1, and after reacting at room temperature f...

Embodiment 3

[0047] As an embodiment of the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides of the present invention, the preparation method of the collagen coating on the titanium alloy surface loaded with antibacterial polypeptides described in this embodiment comprises the following steps:

[0048] (1) Sample pretreatment: Use acetone, ethanol, and pure water to ultrasonically clean the micro-arc oxidation porous titanium alloy in sequence, wash each reagent three times, each time for 10 minutes, and dry in a vacuum oven after cleaning;

[0049] (2) dissolving collagen in hexafluoroisopropanol, stirring magnetically for 3 hours at room temperature, and preparing a collagen solution with a concentration of 0.5 wt %;

[0050] (3) Add a dopamine solution with a concentration of 10 mg / mL to the collagen solution obtained in step (1), wherein the weight ratio of dopamine to collagen is 0.1:1, and after reacting at room temperatur...

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Abstract

The invention discloses a titanium alloy surface collagen coating loaded with antibacterial polypeptides and a preparation method of the coating. An electrostatic spraying technology is also used, thecollagen coating loaded with the antibacterial polypeptides is simultaneously constructed on the surface of porous titanium alloy, the process is simple and convenient, and the spraying efficiency ishigh. Dopamine is oxidized to form polydopamine which has good adhesion, combination of the coating and a titanium alloy matrix can be enhanced, the coating is not likely to fall off, and the coatingcan coexist with a micro-arc oxidation structure on the surface of the porous titanium alloy; meanwhile, the antibacterial polypeptides are loaded on the surface of the titanium alloy; the collagen coating loaded with the antibacterial polypeptides can induce generation of bones and cartilage, more stable chemical bonding and higher biological activity are provided for the titanium alloy implants, the collagen coating is more beneficial for ingrowth of new bone issue, a good antibacterial property is achieved, the infection risk caused by implantation of the titanium alloy can be effectivelyreduced, and the implantation effect is improved.

Description

technical field [0001] The invention relates to the technical field of biomedical coatings, in particular to a collagen coating on the surface of a titanium alloy loaded with antibacterial polypeptides and a preparation method thereof. Background technique [0002] Titanium alloys lack good osteoinductivity, and the inert oxide layer on its surface limits its integration with surrounding bone tissue while reducing host rejection. When titanium alloy materials are implanted in the body, common biological reactions mainly occur on the surface of the implant, so the surface biofunctionalization of titanium alloys has attracted extensive attention from researchers. [0003] The surface biofunctionalization of titanium alloys can not only maintain the mechanical properties of titanium alloys, but also significantly improve the osseointegration performance of implants and improve the success rate of implant surgery. Collagen composite technology on the surface of titanium alloy i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/06A61L27/34A61L27/54A61L27/56A61L27/50B05D1/04B05D7/14B05D7/24
CPCA61L27/06A61L27/34A61L27/56A61L27/54A61L27/50B05D1/04B05D7/14B05D7/24A61L2300/252A61L2300/404A61L2300/606A61L2400/18A61L2420/02A61L2420/06A61L2430/02B05D2202/35C08L89/00
Inventor 董亮亮陈淡嫦李奕恒罗思施
Owner GUANGZHOU TRAUER BIOTECH
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