45 results about "Collagen coating" patented technology

Methods to produce perforated collagen coated meshes for use as implants have been developed. The method involves positioning needles through the pores of the mesh, coating the mesh with a collagen solution, freezing the coated mesh, removing the needles from the frozen coated mesh, drying the collagen coated mesh, and optionally cross-linking the coated mesh. The method allows perforated collagen coated meshes to be prepared with variable thickness, and without damage to the surface of the mesh. The perforations of the collagen coated meshes may be designed to prevent the formation of fluid pockets when the coated meshes are implanted, and to permit rapid incorporation into host tissue. The perforated collagen coated meshes may be used for soft tissue repair, regeneration or remodeling including, for example, hernia repair, mastopexy, treatment of urinary incontinence, pelvic floor reconstruction, and ligament and tendon repair.

A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a microcarrier which becomes magnetic in nature when placed in a the presence of a magnetic field and which facilitates cellular adherence (via the collagen coating) for 3D construct development.

The invention discloses a preparation method of a collagen coating embedded with chitosan microspheres on a medical metal implant surface. The mineralized collagen coating embedded with the chitosan microspheres is formed on a substrate by adopting an electrochemical deposition method and adjusting parameters such as a pH (potential of hydrogen) value and on-load voltage of an electrolytic solution. The method is simple in technology; the prepared coating is embedded with the chitosan microspheres; and as the chitosan microspheres has an effect of effective controlled release of biological factors or drugs such as antibiotics, the coating has high biological responsiveness, a good drug sustained release function and a wide application prospect in the field of hard-bone tissue repair.

The present invention relates to electrochemical deposition process for preparing nanometer ordered CaP/collagen coating on the surface of medical metal product. The present invention provides one kind biological material with bone-like structure and homogeneous chemical property prepared in mild condition, and the material has greatly raised biocompatibility and bioactivity and may be applied clinically. The preparation process includes the following steps: pre-treatment of the substrate, and cathode electrochemical deposition of the substrate inside the calcium phosphate deposition solution with collagen dispersing liquid and pH 3-6 in current density of 0.1-1.0 mA/sq cm and at 50-100 deg.c for 10-30 min.

The invention discloses a preparation method of a biological gradient coating on the surface of a dental implant. The method is used for performing operation of the following steps on the dental implant: polishing a titanium sheet by SiC metallographic abrasive paper, cleaning, drying, and setting the dried titanium sheet on the surface of the dental implant to form a titanium substrate layer; preparing an La-HA/TiO2 composite coating on the surface of the titanium substrate layer; preparing an HA coating on the surface of the La-HA/TiO2 composite coating; and setting the dental implant in an apatite/collagen solution containing La, thus forming the apatite/collagen coating on the surface of the dental implant. According to the invention, the La-HA/TiO2 composite coating is prepared on the titanium surface, so that the excessively high solubility of the HA coating is effectively lowered, the problem of instability between original HA and a metal interface is solved, and the chemical stability and the bonding force of the coating are improved; with the novel coating design, the biological activity and the solubility are improved in a gradient way from inside to outside, and the chemical stability and the bonding strength are improved in a gradient way from outside to inside.

The invention discloses a collagen-coated micro-carrier used in animal cell culture, and a preparation method thereof. According to the micro-carrier provided by the invention, konjac glucomannan microspheres are adopted as a substrate, and are coated by using collagen. The micro-carrier preparation method comprises the steps that: an activating reagent and a catalyst are added into the substrate microspheres, and an activation reaction is carried out in an alkaline environment; the prepared microspheres are cleaned and are vacuum-dried; the microspheres are added into a buffering solution, and collagen is added, such that a coupling reaction is carried out; the system after reaction is well mixed, and is added into water, such that collagen is coated and agglomerated on the surfaces of the microspheres; and the microspheres are washed; the microspheres are added into a reaction vessel; a crosslinking reagent and a PBS buffering solution are added, and the mixture is stirred, such that a reaction is carried out; after reaction, the product is washed, such that the micro-carrier product is obtained. The micro-carrier has uniform collagen coating on the surface, and the collagen is firmly connected, such that cell rapid adhesion and high-density growth can be facilitated. Also, the prepared micro-carrier has the advantages of low cost, short period, and suitability for industrialized productions.

The invention relates to the field of tissue engineering technology, and in particular to a breast repair patch and a preparation method thereof. Problems in the prior that collagen bundles on the surface of a loose layer become free, floating and even falling off are solved; appearance quality of the finished product (the breast repair patch) is improved and service cycle of tissue preservation liquid and preservation cycle of the finished product are prolonged; and meanwhile, the breast repair patch, when used, can facilitate adhesion and fixation of the membrane tissue loose layer, accelerate in-growth and migration of breast cells and tissues, induce generation of new tissues and greatly relieve such symptoms as hard breasts, pain and the like after an operation. According to the embodiment, the breast repair patch provided by the invention comprises a mammal membrane tissue which comprises a dense layer and the loose layer and a collagen coating which is formed on the surface of the loose layer.

The invention provides calcium phosphate bioceramics compounded with collagen as well as a preparation method and a usage method thereof. The preparation method of the calcium phosphate bioceramics compounded with collagen comprises the following steps: forming cross-linked collagen coating on surfaces of calcium phosphate bioceramics by using collagen solutions of which the concentrations are from low to high, thereby obtaining cross-linked collagen coating with which the surfaces of the calcium phosphate bioceramics are wrapped; carrying out mixing with a collagen solution, and carrying outfreeze-drying so as to obtain precursors of the calcium phosphate bioceramics compounded with collagen; and then, soaking the coating with a collagen solution, and carrying out freeze-drying so as toobtain the calcium phosphate bioceramics compounded with collagen which comprise collagen wrapping layers. The calcium phosphate bioceramics compounded with collagen prepared according to the technical scheme of the invention have improved repair ability and degradation performance, so that, aseptic inflammation caused by falling off of calcium phosphate ceramic particles in clinical use can be avoided; moreover, the calcium phosphate bioceramics compounded with collagen are plastic in shape and size as well as convenient to use, so that, many problems of bioceramics in clinical application can be solved.

The invention relates to a method for separating pig spermatogonia stem cells. The method comprises steps of preparing solutions and separating spermatogonia stem cells, wherein in the step of preparing solutions, the solutions to be prepared comprises nutrient solution, digestive enzyme solution, buffer solution, collagen solution, gelatin solution and laminin solution, the step of separating spermatogonia stem cells comprises substeps of cutting pig testicular tissue into pieces and adding enzyme for digestion, filtering by a cell strainer, carrying out enrichment culture and difference adherent culture of the spermatogonia stem cells, finally collecting the spermatogonia stem cells, wherein the difference adherent culture comprises collagen coating adherent culture and laminin coating adherent culture. The method is characterized in that the nutrient solution comprises DMEM/F12, FBS (fetal bovine serum), sodium pyruvate, penicillin and streptomycin; according to the collagen coating adherent culture, after being cultured for required time, the cell is blown and beaten and then continuously cultured. In the collagen coating adherent culture, the spermatogonia stem cell is slightly blown and beaten and is not attached to the wall of the collagen, so that loss of the spermatogonia stem cell is reduced, and the yield of the spermatogonia stem cell is improved.

The invention discloses a titanium alloy surface collagen coating loaded with antibacterial polypeptides and a preparation method of the coating. An electrostatic spraying technology is also used, thecollagen coating loaded with the antibacterial polypeptides is simultaneously constructed on the surface of porous titanium alloy, the process is simple and convenient, and the spraying efficiency ishigh. Dopamine is oxidized to form polydopamine which has good adhesion, combination of the coating and a titanium alloy matrix can be enhanced, the coating is not likely to fall off, and the coatingcan coexist with a micro-arc oxidation structure on the surface of the porous titanium alloy; meanwhile, the antibacterial polypeptides are loaded on the surface of the titanium alloy; the collagen coating loaded with the antibacterial polypeptides can induce generation of bones and cartilage, more stable chemical bonding and higher biological activity are provided for the titanium alloy implants, the collagen coating is more beneficial for ingrowth of new bone issue, a good antibacterial property is achieved, the infection risk caused by implantation of the titanium alloy can be effectivelyreduced, and the implantation effect is improved.

The invention discloses a preparation method for a medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating. The method includes the steps that an electrolyte containing calcium, phosphorus, collagen and chitosan quaternary ammonium salt is prepared; a platinum sheet serves as a counter electrode; a metal substrate washed through acid serves as a work electrode; and electrochemical deposition is performed in a multi-potential-step manner. The positive and negative condition and the amplitude of each step work electrode potential and deposition time of each segment are adjusted, so that deposition does not need to be performed multiple times, the purpose of controllable space distribution of the chitosan quaternary ammonium salt in a mineralized collagen coating on the surface of the substrate can be quickly and easily achieved, and in other words, the inner layer and the outer layer of the collagen coating are rich in chitosan quaternary ammonium salt. The feature that the chitosan quaternary ammonium salt is provided with positive charges and biological growth factors are carried in different space positions of the collagen coating, so that the biological growth factor release behavior can be controlled and adjusted. Meanwhile, the capacity of the coating for carrying biological growth factors is improved and the method has the wide application prospect in hard-bone tissue regeneration field.

The invention discloses a structure-controllable magnetic response mineralized collagen coating used for the surface of a medical metal implant. The structure-controllable magnetic response mineralized collagen coating is constituted by mineralized collagen and Fe3O4 nanometer particles, wherein the Fe3O4 nanometer particles are distributed in the mineralized collagen. The structure-controllable magnetic response mineralized collagen coating adopts a magnetic field assisted alternate electric potential method; by adjusting parameters such as magnetic field strength in the composing and depositing process of an electrolyte, the nanometer magnetic particles are compounded into the mineralized collagen; and a modified layer with the magnetic response structure-controllable mineralized collagen is prepared on the surface of the metal implant. According to a preparation method of the structure-controllable magnetic response mineralized collagen coating, the structure-controllable magnetic response mineralized collagen coating is prepared through effective combination of an outer magnetic field and electrochemical deposition, the cell differentiation action is regulated and controlled, and the structure-controllable magnetic response mineralized collagen coating used for the surface of the medical metal implant has application prospects in restoration of hardbone tissue.

A rotator cuff patch based on polyethylene and a collagen coating comprises a rotator cuff patch body, a UPHWE patch substrate layer, an anti-inflammatory drug loading layer containing one or more collagens of aspirin and celecoxib, and a healing promoting drug loading layer containing one or more collagens of VEGF and TGF beta, and the rotator cuff patch realizes early inhibition of inflammatory response, and has the advantages of high bioavailability, high bioavailability, high bioavailability and high safety. In the repair period, blood vessel formation is promoted to achieve the effect of promoting graft integration.

The invention discloses a PLGA (poly(lactic-co-glycolic acid))-PEG (poly(ethylene glycol))-PLGA inlaid mineralized collagen coating and its preparation method. The method of the invention conducts electrochemical deposition, adjusts deposition parameters, constructs a mineralized collagen coating with a porous structure on a metal implant surface, and inlays a temperature sensitive polymer PLGA-PEG-PLGA into the structure, so that the coating becomes an excellent drug slow release system and can be used for controlled release of biotic factors, antibiotics and other drugs in vivo. PLGA-PEG-PLGA can make sol-gel conversion with temperature variation. On account of the characteristic, biotic factors and antibiotics, etc. are loaded under a sol state of PLGA-PEG-PLGA, under the gel state of which the drugs can be released, thus optimizing the slow release behavior of the coating. The coating prepared by the method of the invention can make use of mineralized collagen to transmit biological signals and the temperature sensitive polymer to control drug release effectively, so that the coating can has high biological responsiveness as well as a good drug slow release function simultaneously, thus boasting wide application prospects in hard bone tissue repair field.

The invention discloses a collagen/hydroxyapatite composite coating joint prosthesis implant and a preparation method thereof. The surface of a joint prosthesis material is coated with collagen and hydroxyapatite composite sequentially, wherein the thickness of the collagen coating is 10-20 mu m, the porosity of the collagen coating is 90-95 percent, the thickness of the hydroxyapatite coating is 20-50 mu m, and the porosity of the hydroxyapatite coating is 5-15 percent, so that the surface of the joint prosthesis contains a natural bone extracellular matrix ingredient, so that the function of inducing osteogenesis is enhanced, and thus the linkage of bone and an implanted titanium plate is promoted, the joint prosthesis is physiologically fixed, and the difficulty that the joint prosthesis is easy to loosen after being implanted inside in the prior art is effectively solved.

The invention provides a preparation method of a titanium-based dental implant material loaded with antibacterial polypeptide and antibacterial ions, a collagen coating loaded with the antibacterial polypeptide and the antibacterial ions is constructed on the surface of the titanium-based dental implant material at the same time, the process is simple and convenient, the cost is low, and the efficiency is high; the dopamine is oxidized to form polydopamine which has good adhesion, and a good intermediate transition layer can be formed on the surface of a matrix. Chemical grafting of the antibacterial polypeptide and chelation of antibacterial ions can be realized by utilizing abundant surface groups of the polydopamine film, so that the synergistic effect of contact antibacterial of the antibacterial polypeptide and antibacterial of the antibacterial ions is realized, the antibacterial efficiency is improved, the antibacterial effect is enhanced, bacterial infection around the implant can be prevented, and the antibacterial effect of the implant is improved. Meanwhile, the antibacterial polypeptide and the antibacterial ions are not prone to causing drug resistance, and the biological activity is guaranteed.

The invention discloses a porous titanium alloy surface bioglass/polycaprolactone/collagen coating and a preparation method thereof. By combining with an electrostatic spraying technology, a bioglass/polycaprolactone/collagen coating is constructed on a porous titanium alloy surface at the same time, the process is simple and convenient, the spraying efficiency is high, the bioglass/polycaprolactone/collagen coating is tightly combined with a titanium alloy substrate and does not easily shed off, and the coating can coexist with the micro-arc oxidation structure on the porous titanium alloy surface; furthermore, the bioglass/polycaprolactone/collagen coating improves the biocompatibility and tissue repair capability of a titanium alloy material, provides more stable chemical bonding and higher bioactivity for a titanium alloy implant, and is more beneficial to the ingrowth of new bone tissue.

The invention discloses a method for efficiently separating intramuscular fibroblast-lipogenic progenitor cells of a mouse. The method comprises the following steps that (1), a collagen coating culture dish is prepared; (2), mouse skeletal muscle is separated and digested; and (3), differential adherence separation is carried out on the FAP. It is determined through cell flow analysis and oil red O staining that the separated cells are high in purity, high in differentiation potential, high in proliferation speed and high in survival rate; by adopting the collagen coating culture dish, cell attachment is facilitated, the separation time is greatly shortened, and the cell yield is improved and the used C57BL/6 mouse is wide in source and easy to obtain, and the difficulty of reproduction of an experimental method is greatly reduced. Compared with a traditional flow sorting method, a flow sorter is not needed, and the method is more economical, practical, convenient and rapid; and a good cell basis is provided for researching the functions of the fibroblast/lipoblastic progenitor cells in development and regeneration of skeletal muscles.