45 results about "Collagen coating" patented technology

The invention herein relates to the field of attaching and securing manmade material onto or into bone in correcting bone defects by implanting stress-bearing bone replacements. In particular, the invention relates to applying a coating of collagen securely onto the implant to ensure the collagen material will not be removed from the implant during installation, such as screwing the anchor into the bone. This addresses the removal of the coatings by the scraping action of the bone on the soft collagen. In the past, it has not been possible to provide adequate adhesive strength to the bone collagen / implant interface or the cohesive force of the collagen itself to prevent the removal of the coating by the abrasive action between bone and known implants.

The invention provides a calcium phosphate bioceramic compounded with collagen and its preparation and use method. The preparation method of the calcium phosphate bioceramic compounded with collagen comprises: using collagen solution from low to high concentration on the surface of calcium phosphate ceramic particles Form a cross-linked collagen coating to obtain a cross-linked collagen coating wrapped on the surface of calcium phosphate ceramic particles, then mix with collagen solution and freeze-dry to obtain the precursor of calcium phosphate bioceramic composite collagen, and then use collagen solution to soak Freeze-dry after coating to obtain calcium phosphate bioceramics with collagen coating. The technical solution of the present invention improves the repair ability and degradation performance of the calcium phosphate bioceramics compounded with collagen, avoids aseptic inflammation caused by the shedding of calcium phosphate ceramic particles during clinical use, and utilizes the plasticity of shape and size and The convenience of use solves many problems encountered in the clinical application of bioceramics.

The present invention relates to electrochemical deposition process for preparing nanometer ordered CaP / collagen coating on the surface of medical metal product. The present invention provides one kind biological material with bone-like structure and homogeneous chemical property prepared in mild condition, and the material has greatly raised biocompatibility and bioactivity and may be applied clinically. The preparation process includes the following steps: pre-treatment of the substrate, and cathode electrochemical deposition of the substrate inside the calcium phosphate deposition solution with collagen dispersing liquid and pH 3-6 in current density of 0.1-1.0 mA / sq cm and at 50-100 deg.c for 10-30 min.

The invention discloses a collagen-coated micro-carrier used in animal cell culture, and a preparation method thereof. According to the micro-carrier provided by the invention, konjac glucomannan microspheres are adopted as a substrate, and are coated by using collagen. The micro-carrier preparation method comprises the steps that: an activating reagent and a catalyst are added into the substrate microspheres, and an activation reaction is carried out in an alkaline environment; the prepared microspheres are cleaned and are vacuum-dried; the microspheres are added into a buffering solution, and collagen is added, such that a coupling reaction is carried out; the system after reaction is well mixed, and is added into water, such that collagen is coated and agglomerated on the surfaces of the microspheres; and the microspheres are washed; the microspheres are added into a reaction vessel; a crosslinking reagent and a PBS buffering solution are added, and the mixture is stirred, such that a reaction is carried out; after reaction, the product is washed, such that the micro-carrier product is obtained. The micro-carrier has uniform collagen coating on the surface, and the collagen is firmly connected, such that cell rapid adhesion and high-density growth can be facilitated. Also, the prepared micro-carrier has the advantages of low cost, short period, and suitability for industrialized productions.

The invention relates to a method for separating pig spermatogonia stem cells. The method comprises steps of preparing solutions and separating spermatogonia stem cells, wherein in the step of preparing solutions, the solutions to be prepared comprises nutrient solution, digestive enzyme solution, buffer solution, collagen solution, gelatin solution and laminin solution, the step of separating spermatogonia stem cells comprises substeps of cutting pig testicular tissue into pieces and adding enzyme for digestion, filtering by a cell strainer, carrying out enrichment culture and difference adherent culture of the spermatogonia stem cells, finally collecting the spermatogonia stem cells, wherein the difference adherent culture comprises collagen coating adherent culture and laminin coating adherent culture. The method is characterized in that the nutrient solution comprises DMEM / F12, FBS (fetal bovine serum), sodium pyruvate, penicillin and streptomycin; according to the collagen coating adherent culture, after being cultured for required time, the cell is blown and beaten and then continuously cultured. In the collagen coating adherent culture, the spermatogonia stem cell is slightly blown and beaten and is not attached to the wall of the collagen, so that loss of the spermatogonia stem cell is reduced, and the yield of the spermatogonia stem cell is improved.

The preparation method of the chitosan quaternary ammonium salt / collagen composite coating on the surface of a medical metal implant disclosed by the invention comprises preparing an electrolyte solution containing calcium, phosphorus, collagen and chitosan quaternary ammonium salt, and using a platinum sheet as a counter electrode , using the acid-cleaned metal substrate as the working electrode, using the multi-potential step mode for electrochemical deposition, by adjusting the positive and negative of the working electrode potential, the size of each step, and the deposition time of each step, without multiple depositions, it can be quickly and easily The controllable spatial distribution of the chitosan quaternary ammonium salt in the mineralized collagen coating on the surface of the substrate is realized, that is, the inner layer of the collagen coating is enriched with the chitosan quaternary ammonium salt and the outer layer of the coating is enriched with the chitosan quaternary ammonium salt. Utilizing the positively charged nature of chitosan quaternary ammonium salt, the biological growth factors are carried on different spatial positions of the collagen coating, which realizes the controllable regulation of the release behavior of biological growth factors, and at the same time increases the ability of the coating to carry biological growth factors , has broad application prospects in the field of hard bone tissue repair.