45 results about "Collagen coating" patented technology

The present invention relates to electrochemical deposition process for preparing nanometer ordered CaP / collagen coating on the surface of medical metal product. The present invention provides one kind biological material with bone-like structure and homogeneous chemical property prepared in mild condition, and the material has greatly raised biocompatibility and bioactivity and may be applied clinically. The preparation process includes the following steps: pre-treatment of the substrate, and cathode electrochemical deposition of the substrate inside the calcium phosphate deposition solution with collagen dispersing liquid and pH 3-6 in current density of 0.1-1.0 mA / sq cm and at 50-100 deg.c for 10-30 min.

The invention discloses a collagen-coated micro-carrier used in animal cell culture, and a preparation method thereof. According to the micro-carrier provided by the invention, konjac glucomannan microspheres are adopted as a substrate, and are coated by using collagen. The micro-carrier preparation method comprises the steps that: an activating reagent and a catalyst are added into the substrate microspheres, and an activation reaction is carried out in an alkaline environment; the prepared microspheres are cleaned and are vacuum-dried; the microspheres are added into a buffering solution, and collagen is added, such that a coupling reaction is carried out; the system after reaction is well mixed, and is added into water, such that collagen is coated and agglomerated on the surfaces of the microspheres; and the microspheres are washed; the microspheres are added into a reaction vessel; a crosslinking reagent and a PBS buffering solution are added, and the mixture is stirred, such that a reaction is carried out; after reaction, the product is washed, such that the micro-carrier product is obtained. The micro-carrier has uniform collagen coating on the surface, and the collagen is firmly connected, such that cell rapid adhesion and high-density growth can be facilitated. Also, the prepared micro-carrier has the advantages of low cost, short period, and suitability for industrialized productions.

The preparation method of the chitosan quaternary ammonium salt / collagen composite coating on the surface of a medical metal implant disclosed by the invention comprises preparing an electrolyte solution containing calcium, phosphorus, collagen and chitosan quaternary ammonium salt, and using a platinum sheet as a counter electrode , using the acid-cleaned metal substrate as the working electrode, using the multi-potential step mode for electrochemical deposition, by adjusting the positive and negative of the working electrode potential, the size of each step, and the deposition time of each step, without multiple depositions, it can be quickly and easily The controllable spatial distribution of the chitosan quaternary ammonium salt in the mineralized collagen coating on the surface of the substrate is realized, that is, the inner layer of the collagen coating is enriched with the chitosan quaternary ammonium salt and the outer layer of the coating is enriched with the chitosan quaternary ammonium salt. Utilizing the positively charged nature of chitosan quaternary ammonium salt, the biological growth factors are carried on different spatial positions of the collagen coating, which realizes the controllable regulation of the release behavior of biological growth factors, and at the same time increases the ability of the coating to carry biological growth factors , has broad application prospects in the field of hard bone tissue repair.