Ferromagnetic cell and tissue culture microcarriers

Abstract

A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a microcarrier which becomes magnetic in nature when placed in a the presence of a magnetic field and which facilitates cellular adherence (via the collagen coating) for 3D construct development.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application is a continuation of U.S. application Ser. No. 11 / 307,077, filed Jan. 23, 2006, which is a continuation of International Application No. PCT / US2004 / 023746, filed Jul. 23, 2004, which claims the benefit of U.S. Provisional Application Ser. No. 60 / 481,126, filed Jul. 23, 2003.BACKGROUND OF THE INVENTION[0002]It has long been established that cells and tissue growing in microgravity behave differently than those on Earth. The ongoing challenge for the experimental study of cell behavior under these conditions has been simulating the environment of microgravity so that complete laboratory studies can be conducted on Earth. This provides the obvious advantages of cost-effectiveness and safety.[0003]To address this issue, NASA developed the bioreactor in the 1980s. Essentially, the bioreactor is a cylindrical vessel equipped with a membrane for gas exchange and ports for media exchange and sampling. As the bioreactor tur...

AI Technical Summary

Benefits of technology

The present invention provides a method for creating a three-dimensional cell culture model by using microcarriers, graphite plates, and a magnet. The cells are suspended in a tissue culture bag and held in suspension using magnetic forces. This allows for the study of cell growth in a constant and nonrandomized environment. The method can also involve using ferromagnetic microcarriers and pyrolytic graphite plates. This method can be performed in a standard incubator for continued cellular construction. The technical effect is the ability to create a stable and controlled environment for studying cell growth in a 3D model.

Problems solved by technology

The ongoing challenge for the experimental study of cell behavior under these conditions has been simulating the environment of microgravity so that complete laboratory studies can be conducted on Earth.

Unfortunately however, on Earth the clusters become too large to fall slowly.

In ground based studies, however, it has been difficult to separate these parameters from one another in order to examine the influence of each on three-dimensional cellular growth and function.

Commercially available magnetic beads are either too small for use as microcarriers in cell culture (diameters on the order of <10 um which are not feasible for use as cell culture supports), and / or they lack appropriate surface matrix coating to facilitate cell adherence, a requisite factor to maintain cell-cell interactions for 3D construct development during in vitro culture.

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