296results about "Microcarriers" patented technology

The invention relates to a cell culture microcarrier as well as a preparation method and application thereof. The cell culture microcarrier comprises a polymer porous microsphere with an inside-outside through pore channel structure. The cell culture micro-carrier comprises a biodegradable micro-carrier and also comprises a non-degradable micro-carrier, so that the growth of cells in the carrier is facilitated, and the mass transfer of nutrient substances and metabolites is also facilitated, thereby promoting the growth of the cells, enabling the cultured cells to keep good activity and function, and being high in proliferation efficiency; the method is suitable for three-dimensional culture of somatic cells, stem cells, tumor cells and the like.

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The invention discloses a multi-brain organ electron complex as well as a construction method and application thereof. The multi-brain organ electronic complex comprises a microelectrode array and a fusion organ attached to the surface of a chip electrode of the microelectrode array through a PDMS film, and the fusion organ can grow for more than or equal to 120 days through culture. The PDMS film is attached to the surface of the MEA chip electrode, so that the stability of combination of the MEA chip electrode and the organoid is remarkably enhanced, the construction efficiency of a multi-brain organoid electron complex is improved, and the continuity of neural signal detection is improved; a high-performance model platform is provided for multi-brain organ research, and the method has a wide application prospect.

The invention discloses a preparation method of a mesenchymal stem cell exosome based on microcarrier three-dimensional culture and application of the mesenchymal stem cell exosome in anti-aging skin care products. According to the method, a three-dimensional culture system of mesenchymal stem cells is optimized, and the anti-aging function activity of the subsequently separated and purified exosome is remarkably improved by adopting a microcarrier with recombinant collagen as a main component; subsequently, by optimizing an exosome separation and purification technology, and through a specific separation and purification mode and conditions, the exosome with anti-aging active ingredients is further obtained. Experiments prove that the exosome obtained by the method disclosed by the invention can effectively remove metalloproteinase 1 and promote collagen regeneration, has a remarkable anti-aging effect, is suitable for essence, face cream and other dosage forms, and has a wide market prospect.

To provide non-naturally occurring three-dimensional (3D) brown adipose-derived stem cell aggregates, and methods of making and using the same.SOLUTION: The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates. Also provided herein is an encapsulation system comprising non-naturally occurring 3D brown adipose-derived stem cell aggregates. The 3D brown adipose-derived stem cell aggregate may comprise brown adipose-derived stem cells that express one or more brown adipocyte gene in the absence of differentiation medium.SELECTED DRAWING: None

The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates.

The invention relates to the field of biological medicine, in particular to a water-soluble alginate microcarrier as well as a preparation method and application thereof. The alginate microcarrier comprises a carrier core structure and a shell surface modifier, the micro-carrier core layer is formed by carrying out plasma crosslinking on water-soluble alginate, ethylenediamine tetraacetic acid salt, nitrilotriacetic acid salt, diethylene triamine pentaacetic acid and water-soluble divalent metal salt, and light-sensitive alginate and a photoinitiator can also be added, so that the micro-carrier core layer has two crosslinking networks; the shell layer is composed of one of gelatin, collagen, fibrinogen, albumin and fibrin, or the gelatin, the collagen, the fibrinogen, the albumin and the fibrin and other biocompatible polymers; the obtained microcarrier has the characteristics of high transparency and rapid degradation, and adherent cells can be amplified in vitro; the microcarrier obtained in the invention can be used for mesenchymal stem cell culture, and can be rapidly degraded under a mild cell condition to harvest cells.

The invention discloses a natural product-induced artificial nano-vesicle derived from human umbilical cord mesenchymal stem cells and application of the natural product-induced artificial nano-vesicle in ischemic diseases. The preparation method of the nano vesicle derived from the human umbilical cord mesenchymal stem cells comprises the following steps: S1, inoculating the human umbilical cord mesenchymal stem cells to a 3D microcarrier; then adding traditional Chinese medicine active ingredients to activate the human umbilical cord mesenchymal stem cells, continuing to culture, cracking the microcarrier, and harvesting cells; s2, dispersing the human umbilical cord mesenchymal stem cells obtained in S1 to obtain a suspension, extruding the suspension through a polycarbonate film, and then discarding large particles by adopting a gradient centrifugation method to obtain the nano-vesicles. Compared with naturally secreted exosomes, the exosome has the advantages that the yield is increased, the treatment efficiency is improved, the production cost is reduced, and compared with stem cells, the problems of low transplantation rate and the like are avoided. The stem cells are pretreated based on the traditional Chinese medicine, the vesicles are extracted by utilizing an extrusion method, and the yield and the treatment efficiency of the nano vesicles are remarkably improved.

Edible microcarrier for growing anchorage-dependent cells to prepare a cultured meat product, where the edible microcarrier includes a microcarrier core and a microcarrier coating at least partially directly or indirectly covering the surface of said microcarrier core, wherein said microcarrier core is a hydrogel having only the following components: (A) 0.03-10.0 wt % of at least one ionically crosslinked polysaccharide; (B) 0.05-5 wt % of a mechanical stability agent different from (A); (C) 0.0-10 wt % of additives different from (A) and (B); (D) 0.0-50 wt % living cells; (E) 75-99.92 wt % of water; where the sum of (A)-(E) making up 100 wt %, and where the microcarrier coating includes a biopolymer, where the biopolymer is crosslinked using a crosslinking agent.

The invention discloses a preparation method and application of a multi-material cell-loaded 3D scaffold, and the preparation method comprises the following steps: S1, preparing at least two kinds of printing ink; s2, various printing inks prepared in the step S1 are added into 3D printing equipment, and a 3D support is printed according to a designed model; the printing ink comprises a main material and a photoinitiator, the main material comprises one or a mixture of a photosensitive material and a non-photosensitive material, and at least one kind of printing ink further comprises a cell-loaded suspension. The processing and manufacturing efficiency and convenience can be improved, the manufacturing cost is reduced, meanwhile, dynamic culture and testing of the carrier cells are achieved, and the scientific research efficiency is improved.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

The invention discloses application of a 3D hypoxia mesenchymal stem cell derived exosome preparation in preparation of an immune cell proliferation inhibitor or a medicine for treating acute myocardial infarction, belongs to the technical field of biology, and solves the problems of low cell survival rate and immunological rejection in application of a cell treatment method in the prior art. The 3D hypoxia mesenchymal stem cell derived exosome is used as a medium for treating acute myocardial infarction, and the problem of immunological rejection can be solved.

The invention discloses a freeze-dried human rabies vaccine and a preparation method thereof, and belongs to the technical field of viral vaccines. The method comprises the following steps: (1) cell culture; (2) virus inoculation; (3) harvesting viruses; (4) concentration and purification: clarifying the harvested virus liquid through a filter, carrying out ultrafiltration concentration through an ultrafiltration membrane to obtain a concentrated solution, and purifying by adopting a column chromatography to obtain a chromatographic solution; (5) preparing a stock solution: inactivating and hydrolyzing the chromatographic solution to obtain a virus inactivation solution, and adding human serum albumin into the virus inactivation solution to obtain the stock solution; (6) preparing a semi-finished product: diluting with a stabilizer to prepare the semi-finished product, wherein the stabilizer comprises the following raw materials: human serum albumin, araboxylan, L-arginine hydrochloride, casein phosphopeptides, alpha-tocopherol nicotinate, a solvent and the like; and (7) preparing a finished product. The rabies vaccine disclosed by the invention is excellent in quality and stable in immune efficacy, and liquid is clear after redissolution.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

The invention discloses a porous microcarrier capable of loading human umbilical cord mesenchymal stem cells and a preparation method and application of the porous microcarrier, and relates to the technical field of biomedical engineering. Comprising the following raw materials in parts by weight: 0.1-5 parts of methylacryloylated gelatin, 0.1-5 parts of methylacryloylated silk fibroin and 0.1-5 parts of methylacryloylated chondroitin sulfate. The preparation method of the porous microcarrier comprises the following steps: S1, constructing a microfluidic platform; s2, preparing microsphere liquid drops; s3, preparing a porous microcarrier containing gelatin, silk fibroin and chondroitin sulfate; according to the prepared gelatin-silk fibroin-chondroitin sulfate microcarrier capable of loading the hUMSCs, the silk fibroin and the chondroitin sulfate are doped in the gelatin, and compared with the gelatin microcarrier, by adjusting the physical performance of the gelatin-silk fibroin-chondroitin sulfate microcarrier capable of loading the hUMSCs, the anti-inflammatory capacity and the immune regulation capacity of the hUMSCs can be effectively improved, and the capacity of stem cells for treating related diseases is improved.

The embodiment of the invention discloses a method for constructing a cartilage micro-tissue by compounding an extracellular matrix with a vascular matrix component and application. The method comprises the following steps: preparing a porcine decellularized cartilage extracellular matrix; generating a cartilage extracellular matrix suspension; carrying out screening treatment on the cartilage extracellular matrix suspension; preparing a porcine acellular cartilage extracellular matrix microcarrier; preparing a target suspension of a common system from the porcine acellular cartilage extracellular matrix microcarrier and vascular matrix component cells, and transferring the target suspension to a bionic floating microenvironment of a shaker bioreactor for dynamic culture; carrying out culture treatment on the porcine acellular cartilage extracellular matrix microcarrier; and taking out the cell-loaded porcine decellularized cartilage extracellular matrix microcarrier from the cultured and treated porcine decellularized cartilage extracellular matrix microcarrier as the constructed cartilage micro-tissue. According to the embodiment, the condition of secondary injury to a user can be improved and reduced, the repairing effect is improved, and the adaptability of the constructed cartilage micro-tissue is improved.

Provided are methods of obtaining large scale cultures of muscle-derived muscle precursor cells (MPCs) using microcarriers as growth matrices, methods for obtaining therapeutically effective amounts of these cells, populations of cells obtained by the methods, and compositions comprising expanded cells, and a method for preparing a medicament, for example for the treatment of skeletal muscle dysfunction.

A method of increasing lipid synthesis or accumulation in and optionally secretion from a mammary epithelial cell (MEC) culture is provided. The method contacts the MEC with an LXR agonist in the presence of a fatty acid to increase lipid synthesis or accumulation and optionally increase lipid secretion. Also provided is a method of producing synthetic fat globules, the method comprising: (a) isolating lipid droplets from cultured cells; (b) encapsulating the droplets with a lipid bilayer, thereby producing synthetic fat globules. Compositions thus produced are also provided.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

The invention relates to a preparation method of a Vero cell influenza virus vaccine, and belongs to the technical field of virus culture. In the culture of the influenza virus, red algae protein and soybean protein hydrolysate are added into a culture medium at the same time, the red algae protein and soybean protein hydrolysate are subjected to low-temperature ammonium sulfate precipitation and PD-10 desalination column purification, natural conformation is kept, integrin-FAK-Src and IGF-1R signal channels can be activated, and cell adhesion and proliferation are promoted; the latter contains soybean protein isolate, polysaccharide and RGD motif peptide, and can form an adhesion film on a substrate and mediate adhesion through integrin. According to the invention, the Vero cells and the recombinant plasmid are combined to improve cell adherence, metabolic homeostasis and oxidation resistance, so that the Vero cells grow at high density and efficiently replicate influenza viruses under a serum-free condition, the virus titer (TCID50) reaches 8.5 log10 / mL, and the Vero cells and the recombinant plasmid are suitable for industrial vaccine production.

The invention belongs to the field of biological pharmacy, and discloses an MSCs (mesenchymal stem cells) culture process for improving the yield of protein polymers, which comprises the following steps: resuscitating and inoculating mesenchymal stem cells, transferring the mesenchymal stem cells into a culture container added with a microcarrier for rotating or shaking culture, and aseptically transferring the cultured mesenchymal stem cells into a culture bag; performing irradiation stimulation on the culture bag; after stimulation is completed, the mesenchymal stem cells are split, separated and purified, and the protein polymer is obtained. According to the MSCs culture process of some examples, more MSCs can be obtained, and more protein polymers can be obtained through purification.

The invention discloses a preparation method of a micron-sized cell-matrix co-polymer biological material. The preparation method comprises the following steps: firstly, inducing SHED cells in an ultra-low adsorption plate by using an alpha-MEM culture medium containing serum, GlutaMAXI, vitamin C and the like to form micron-sized spheres (SHED spheres); secondly, taking human dentin, and carrying out mechanical crushing, EDTA (Ethylene Diamine Tetraacetic Acid) demineralization, grinding and sterilization to prepare 1-100 microns of dentin matrix particles (DDMPs); and finally, by taking the GelMA hydrogel as a carrier, entrapping SHED spphere and DDMPs, and carrying out 405nm photo-crosslinking for 30 seconds to form the hydrogel. The co-polymer can significantly promote angiogenesis and osteogenesis coupling regeneration in a bone defect model, and is suitable for oral cavity and cranio-maxillofacial bone repair.

A cell culture microcarrier bead is proposed. The microcarrier bead comprises a bead body having its surface flecked with plasmonic nanoparticles. In a second aspect, the invention relates to a cell culture reactor, containing a cell culture medium and the proposed microcarrier beads. A third aspect of the invention concerns a method for observing living cells on such microcarrier beads. Yet a further aspect of the invention relates to a method for packing nanoparticles on a carrier body.

According to the present invention, the present invention relates to a polymer microparticle having a core-shell structure, which comprises: a core comprising a first biocompatible polymer, a metal ion, and an organic crosslinker having one or more reactive functional groups; and a shell surrounding all or a part of the core and comprising a second biocompatible polymer, a metal ion, and an organic crosslinker having one or more reactive functional groups; and a cell attachment-inducing layer formed on the surface of the polymer microparticle; and a microcarrier, a cell complex comprising the same, a medical composition, a cosmetic composition, a medical product, and a cosmetic product.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroidal or spherical shape or substantially ovoid, cylindrical, spheroidal or spherical shape, the smallest dimension of which is between 200 and 400 μm, comprising an external hydrogel layer defining an internal part, said internal part having a granzyme B content of less than 1 μg / ml of medium and comprising between 500 and 5000 lymphocytes forming a three-dimensionally grouped culture. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

To provide a method for culturing stem cells with improved efficiency by using a gel that has improved structural strength and enables long-term and stable release of contents.SOLUTION: A bilayer composite hydrogel microcarrier includes: an inner layer gel structure obtained by ionically crosslinking an inner layer polymer with an inner layer crosslinker; and an outer layer gel structure obtained by covalently crosslinking an outer layer monomer with an outer layer crosslinker, which covers the inner layer gel structure; a first inner layer polymer being sodium alginate, a second inner layer polymer being carboxymethylcellulose, and a nutritional component being located inside the inner layer gel structure. The growth rate of stem cells is improved by controlling the composition of the bilayer composite hydrogel microcarrier, the weight ratio of sodium alginate to carboxymethylcellulose, and the location of the nutritional component. The structural strength can be ensured by controlling the weight percentage of carboxymethylcellulose.SELECTED DRAWING: Figure 2

A coated surface with a polymer substantially consisting of a succession of monomers according to the formula (I) and its use for growing cells.

Provided herein are methods for culturing animal cells in a culture medium comprising a culture ingredient that is composed of or produced by yeast. In particular, the culture ingredient comprises, co

The present invention relates to a method for culturing stem cells using a microcarrier.