714 results about "Extracellular vesicles" patented technology

The invention discloses a hydrogel-acellular matrix composite patch containing functionalized extracellular vesicles and a preparation method of the hydrogel-acellular matrix composite patch. The composite patch comprises a hydrogel layer and an acellular pericardium matrix material layer, and an extracellular vesicle-macrophage membrane fusion body (EV-MM) is entrapped in the cross-linked hydrogel. From two aspects of improving the targeting of EVs to the cardiac infarction part and enhancing the retention of the EVs at the cardiac infarction part, the distribution condition of the EVs at the cardiac infarction part is improved, so that the action efficiency of the EVs is improved, and the treatment effect is improved.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention provides a method for detecting an external vesicle marker through high-flux nano plasma exciting light immune color development, and belongs to the technical field of human extracellular vesicles. After a serum sample is subjected to centrifugal treatment, the serum sample and a CD81 capture antibody substrate are incubated, and the particle size distribution of the vesicles is monitored in real time; a zwitterionic polymer modified gold nanoparticle LAM detection probe and a polyethylene glycol modified silver nanoparticle LprG detection probe are prepared to be specifically combined with a vesicle surface antigen, a chromogenic enhancement solution is adopted to induce a plasma resonance signal, and full-hole scanning imaging is carried out; and constructing a double-layer game optimization model to cooperatively optimize the detection sensitivity and the signal stability, carrying out weighted summation on normalized signals of the particle size subgroups to obtain comprehensive detection signal intensity, comparing the comprehensive detection signal intensity with a threshold value, and outputting a final judgment result. The technical problem that quantitative accuracy is affected by signal intensity deviation caused by vesicle particle size difference in outer vesicle marker detection is solved.

The invention discloses a kit for detecting miRNAs of multiple extracellular vesicles based on a TtAgo-driven rolling circle amplification technology and application of the kit, a platform combines TtAgo protein and RCA reaction, cooperates with endonuclease activity and DNA polymerase activity of TtAgo, accurately controls reaction kinetic balance between temperature and enzyme activity, not only does not damage the cutting activity of TtAgo, but also can detect miRNAs of multiple extracellular vesicles. The method provided by the invention can be used for detecting multiple extracellular vesicles miRNAs, ensures strong polymerization amplification performance, creates a strong one-step single-tube experiment, greatly simplifies the experimental process, shortens the detection time, realizes ultra-sensitive and specific detection of nucleic acid markers, and can be used for detecting multiple extracellular vesicles miRNAs.

The invention discloses application of plant-derived extracellular vesicles in preparation of a product for preventing and treating photoaging and a topical application composition. Pseudo-ginseng is used as a raw material for extraction and purification of plant-derived extracellular vesicles, and results show that the pseudo-ginseng-derived extracellular vesicles can significantly treat or improve photoaging. The invention explores the practical application of the fresh pseudo-ginseng extracellular vesicles based on the traditional theory of traditional Chinese medicine, and clarifies the molecular mechanism of the pseudo-ginseng extracellular vesicles. In a word, the extracellular vesicles derived from pseudo-ginseng have a wide application prospect in treatment of skin aging.

The invention relates to the technical field of biomedical materials, in particular to engineered small extracellular vesicle hydrogel enriched with PD-L1 and Siglec-15 as well as a preparation method and application of the engineered small extracellular vesicle hydrogel. The engineering small extracellular vesicle hydrogel is enriched with PD-L1 and Siglec-15 at the same time, the engineering small extracellular vesicle hydrogel comprises a hydrogel base body and PDL1-Siglec15-sEVs loaded in the hydrogel base body, and the PDL1-Siglec15-sEVs are small extracellular vesicles with the PD-L1 and the Siglec-15 in an overexpression mode. Compared with natural small extracellular vesicles and a traditional wound surface treatment strategy, the engineered small extracellular vesicle hydrogel has the advantages that PD-L1 and Siglec-15 can be enriched in the vesicles, so that more accurate immune microenvironment regulation and control can be realized on the local part of a wound surface, excessive inflammatory response is moderately inhibited, phenotype transformation of macrophages to be beneficial to tissue regeneration is promoted, and the wound surface treatment effect is improved. The hydrogel is used as a carrier and can provide a moist healing environment and a three-dimensional scaffold structure, so that the residence time of the engineered small extracellular vesicles on the local wound surface is remarkably prolonged, and slow release is realized.

The invention belongs to the field of biotechnology and aquaculture, and particularly relates to a method for improving hypoxia resistance of pelteobagrus vachelli based on hypoxia induction of liver extracellular vesicles of the pelteobagrus vachelli. The method comprises the following steps: extracting high-purity extracellular vesicles EVs in liver tissues of pelteobagrus vachelli subjected to low-oxygen treatment by adopting a method of combining differential centrifugation and high-precision iodixanol density gradient centrifugation; diluting the extracted EVs to 2mu g / mu L by using PBS (Phosphate Buffer Solution) to prepare an EVs suspension; an intraperitoneal injection mode is adopted, the EVs suspension is injected into the body of the pelteobagrus vachelli, and after injection is conducted for 24 hours, the hypoxia resistance of the pelteobagrus vachelli is evaluated through a suffocation point detection test. The invention opens up a brand new path for improving the hypoxia resistance of the pelteobagrus vachelli, provides a new idea and a new method for efficiently enhancing the hypoxia resistance of economic fishes in the field of aquaculture, is expected to play an important role in actual aquaculture production, and assists the sustainable development of the aquaculture industry.

The invention discloses application of tea extracellular vesicles in preparation of a product for preventing and / or treating UVB-induced skin photoaging, and belongs to the technical field of application of the tea extracellular vesicles. The tea extracellular vesicles are derived from tea leaves and / or stem leaves and are prepared by a method of combining enzymolysis, multi-stage filtration and ultracentrifugation, and the particle size of the tea extracellular vesicles is mainly distributed in a range of 60-100nm. Experiments show that the tea extracellular vesicles can effectively reduce active oxygen accumulation and lipid peroxidation caused by UVB radiation, repair mitochondrial membrane potential and inhibit expression of matrix metalloproteinase and inflammatory factors, so that photoaging symptoms such as skin wrinkles, erythema and barrier function damage are relieved. The tea extracellular vesicle provided by the invention has the advantages of natural raw materials, good biocompatibility, small side effect and the like, can be used for preparing a plurality of external dosage forms such as gel, cream, spray and the like, and provides a safe and effective new natural product choice for prevention and treatment of skin photoaging.

The invention provides application of tea extracellular vesicles in preparation of drugs for relieving or treating allergic respiratory diseases, and belongs to the technical field of extracellular vesicles. The invention provides application of tea extracellular vesicles in preparation of drugs for relieving or treating allergic respiratory diseases. The tea extracellular vesicles are proved to be capable of remarkably improving related symptoms of allergic respiratory diseases including allergic rhinitis, have good anti-inflammatory and immunoregulation effects, and have the application potential of being used as natural active ingredients for preparing drugs or nasal delivery preparations for treating the allergic respiratory diseases. Compared with existing hormone drugs, the tea extracellular vesicles have the advantages of being natural in source, high in safety, low in side effect and the like; moreover, the extracellular vesicles have nanoscale particle sizes, good mucous membrane permeability and bioavailability, can improve the curative effect, and are suitable for long-term intervention.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention relates to a bioactivity-related characteristic marker of umbilical cord mesenchymal stem cell-derived small extracellular vesicles and application of the bioactivity-related characteristic marker. The bioactivity-related characteristic marker of the umbilical cord mesenchymal stem cell-derived small extracellular vesicles comprises one or a combination of more than two of a PSMB3 protein, an SAA4 protein, a VPS33B protein and an AKR7A2 protein. The invention provides a screening method of the characteristic markers, and particularly, the biological activity of the human umbilical cord mesenchymal stem cell source extracellular vesicles can be effectively judged by detecting the characteristic markers or the combination of the characteristic markers of the umbilical cord mesenchymal stem cell source extracellular vesicles. An evaluation means and an important reference are provided for product quality control, clinical transformation and the like based on the umbilical cord mesenchymal stem cell-derived small extracellular vesicles.

The invention relates to application of a plant-derived extracellular vesicle and a compound thereof in preparation of a pharmaceutical composition for treating or preventing diseases caused by high colonization degree of gram-positive bacteria in skin, in particular to application of a purslane extracellular vesicle and a compound of the purslane extracellular vesicle and hydrogel.

The invention relates to the technical field of extracellular vesicles, in particular to an extracellular vesicle active drug loading method which comprises the following preparation steps: S1, collecting cell supernatant, collecting the cell supernatant under the condition of 4 DEG C, and performing multi-stage centrifugation to remove impurities to obtain purified extracellular vesicles; s2, dissolving a target drug in a corresponding solvent to prepare a drug solution with the concentration of 0.1-5 mg / mL; s3, mixing the purified extracellular vesicles obtained in S1 with the medicine solution obtained in S2 according to the volume ratio of 1: 1, and performing low-frequency ultrasonic treatment in a pulse mode under the ice bath condition of 4 DEG C; s4, incubating the mixed system subjected to ultrasonic treatment at 37 DEG C for 25-35 minutes, and meanwhile, adding a cholesterol solution with the final concentration of 0.005-0.01% to assist membrane repair; the problems of contradiction between the drug loading efficiency and the integrity of the extracellular vesicles, poor drug universality, low purification efficiency and insufficient application stability in the existing active drug loading technology of the extracellular vesicles are specifically solved through the whole process optimization of precise purification, efficient drug loading, directional repair and deep refining.

The invention discloses a preparation method of a plant source nano vesicle and the plant source nano vesicle. According to the preparation method disclosed by the invention, through extensive and deep research and a large amount of screening optimization, the coix seed-derived nano vesicles (SCDNVs) and the gastrodia elata-derived nano vesicles (RGDNVs) which are high in concentration and high in quality are successfully extracted by using the preparation method disclosed by the invention; meanwhile, the invention further researches the anti-tumor effect and the anti-oxidation effect of the prepared plant source nano vesicle. Through screening of conditions of the preparation method, an ultrasonic combined centrifugal method (further comprising screening of ultrasonic conditions, such as ultrasonic power / ultrasonic time and the like) adopted in the invention can overcome the defects that the membrane structure of the vesicle is damaged and the form of the vesicle is not complete enough in the traditional method; therefore, the plant extracellular vesicles which are small in loss, high in concentration, good in stability, more uniform in vesicle particle size, small in particle size span, high in yield and high in quality are obtained.

The invention discloses a preparation method of vascular tissue-derived extracellular vesicles, and belongs to the field of cytobiology. According to the preparation method of the extracellular vesicles, the artery blood vessel tissue is directly subjected to enzymolysis through collagenase, the extracellular vesicles are obtained by combining low-speed centrifugation and ultra-speed centrifugation methods and utilizing a differential centrifugation mode, and the technical blank of obtaining the extracellular vesicles from the blood vessel tissue is filled. Experimental results show that the extracellular vesicles can be successfully prepared from collagenase I, collagenase II, collagenase IV, collagenase V or collagenase IV combined with elastin, the prepared extracellular vesicles are different in particle size, form, yield, protein quantity, purity and the like, and the quality of the extracellular vesicles obtained by enzymolysis of collagenase IV combined with elastin is optimal. The extracellular vesicles are extracted from vascular tissues with normal physiological characteristics for the first time, a new source is provided for preparation of the extracellular vesicles, and the extracellular vesicles have great significance in expanding application of the extracellular vesicles.

The present disclosure relates to a hydrogel ink comprising a lipid particle, a polynucleotide supported within the lipid particle, and a bio-ink. In one embodiment, the lipid particles comprise extracellular vesicles. The disclosure also relates to hydrogels thereof and their use in treating neurological diseases or conditions or regenerating soft tissue.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.

Disclosed herein are engineered neutrophil extracellular vesicles comprising a cancer- or tumor cell-targeting Chimeric antigen receptor, a therapeutic composition, and at least one miRNA. Also disclosed herein are methods of reducing or inhibiting growth of a cancer or tumor by administering to a subject positive for the cancer or tumor the engineered neutrophil extracellular vesicles disclosed herein.

An object of the present invention is to provide a formulation effective for activation of hair dermal papilla cells. Provided are a composition for activation of hair dermal papilla cells, a composition for hair regrowth promotion or hair growth, a composition for hair quality improvement, and a composition for FGF-2, FGF-7 or VEGF production promotion, containing a culture supernatant and / or extracellular vesicles of a microalga.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

This invention discloses a composite microsphere loaded with ZIF-8 nanoparticles and stem cell extracellular vesicles, its preparation method, and its applications. The composite microsphere comprises a hydrogel matrix microsphere, and ZIF-8 nanoparticles and small extracellular vesicles derived from bone marrow mesenchymal stem cells uniformly dispersed and encapsulated within the hydrogel matrix microsphere. This invention integrates ZIF-8 nanoparticles and stem cell-derived sEVs into injectable, photocurable GelMA microspheres, constructing an integrated delivery platform capable of sequential release and multi-stage synergistic effects. This platform successfully integrates and leverages three major functions: immunomodulation, stem cell recruitment, and cartilage-induced differentiation, ultimately achieving high-quality functional healing of the tendon-bone interface at both histological and biomechanical levels, providing a highly promising new strategy for the regeneration and repair of interfacial tissues in the musculoskeletal system.

The current invention relates to isolated Extracellular Vesicles (EVs) wherein said EVs comprise the miRNA markers hsa-miR-125b-5p, hsa-miR-199a-3p, hsa-miR-199b-3p and hsa-miR-16-5p.

The invention relates to a polypeptide for promoting secretion of mesenchymal stem cell vesicles. The amino acid sequence of the polypeptide is RKRPGLLKFLG (SEQ ID NO. 1). The polypeptide is combined with cell membranes of mesenchymal stem cells and induces local cell membrane curvature increase, and formation and secretion of extracellular vesicles are remarkably promoted. The method for promoting the stem cells to secrete the vesicles does not need genetic modification or complex equipment, is easy and convenient to operate, high in safety and suitable for large-scale preparation of the stem cell vesicles and development of related products.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma / bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow / platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate / platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate / platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma / bone marrow concentrate fractions.

The invention provides a method for detecting fusion of extracellular vesicles by adopting fluorescence resonance energy transfer, and belongs to the technical field of extracellular vesicles. A dynamic fluorescence monitoring system is established, low-power laser excitation is adopted, dual-wavelength fluorescence signals are monitored at the same time, fluorescence signal data are collected, graded gain regulation and control processing is carried out through five threshold values, and a fluorescence signal super-sparse matrix is constructed; establishing a fluorescence signal processing matrix model, converting the super-sparse matrix into a dense matrix, constructing a gain matrix for signal correction, calculating a fluorescence resonance energy transfer efficiency parameter, obtaining a standardized efficiency value through matrix operation, outputting an extracellular vesicle fusion kinetic analysis result, and establishing a quantitative evaluation system. The technical problems of poor signal stability and low detection precision in the process of fluorescence resonance energy transfer detection of extracellular vesicle fusion are solved.

The present invention provides a method of recovering an extracellular vesicle from an extracellular vesicle-containing sample. More specifically, the present invention provides a method of recovering the extracellular vesicle that includes separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.

The present disclosure describes anti-inflammatory properties of red blood cell extracellular vesicles, e.g., as mediated by heme, hemoglobin and / or phosphatidylserine content. The present disclosure describes technologies useful for the treatment of inflammatory diseases, disorders, or conditions (e.g., atherosclerosis).

This invention discloses a strain of Bifidobacterium animalis subspecies TRT-2A with allergy-relieving effects and its extracellular vesicle applications. The strain TRT-2A is deposited at the China Center for Type Culture Collection (CCTCC) on November 21, 2025, with accession number CCTCC NO:M 20252645. This strain is highly acid-resistant and exhibits good tolerance to artificial gastric and intestinal fluids. It effectively inhibits bacteria, particularly Streptococcus pyogenes, Streptococcus agalactiae, and / or Escherichia coli. Experimental results show that this strain can alleviate hormone-dependent dermatitis and effectively improve dermatitis symptoms such as erythema and desquamation. Furthermore, extracellular vesicles prepared using TRT-2A can effectively improve skin elasticity and reduce wrinkles. Therefore, the Bifidobacterium animalis subspecies TRT-2A of this invention has multiple functions and is worthy of further promotion and application.