3 results about "Vesicle/vacuole" patented technology

The invention is applicable to the technical field of biological medicine, and provides a resveratrol carbon dot-loaded cell membrane bionic nano-vesicle and application thereof, and the resveratrol carbon dot-loaded cell membrane bionic nano-vesicle is prepared by the following preparation process: mixing pure trans-resveratrol with ultrapure water, adjusting the pH value until complete dissolution, reacting the solution to obtain a carbon dot solution, centrifuging, filtering with a filter membrane, dialyzing and purifying to obtain the resveratrol carbon dot-loaded cell membrane bionic nano-vesicle. Carrying out rotary evaporation and freeze-drying to obtain RES CDs; and dropwise adding Cy7 into the DSPE-PEG solution, stirring overnight, dialyzing to remove free Cy7 to obtain Cy7-coated PEG, and mixing TM4-M, RES CDs and Cy7-coated PEG to obtain the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles RCD-coated PCy-coated EVs. The invention also discloses a preparation method of the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles RCD-coated PCy-coated EVs and application of the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles. The vesicles based on the supporting cell membrane prepared by the invention can specifically deliver the resveratrol carbon dots to the blood testis barrier and treat spermatogenesis disorder.

The present invention relates to a method for preparing Gram-negative bacterial outer membrane-derived nanovesicles (OMNVs) and uses thereof. Additionally, the present invention provides a method for treating or diagnosing cancer using OMNVs. Furthermore, the present invention provides a method for delivering therapeutic or diagnostic substances to target cells or tissues by loading them into OMNVs. Also, the present invention provides a method for preventing and treating various diseases, including cancer and bacterial / viral infections, by co-administering OMNVs with antigens or administering OMNVs expressing antigens.

The invention provides a preparation method of nanoscale artificial stem cell vesicles, and belongs to the technical field of cell vesicles, stem cells repeatedly pass through filter membranes with different apertures, the stem cells are segmented into numerous nanoscale cell vesicles by utilizing extrusion and cutting force when the stem cells pass through the filter membranes, and substances wrapped in the stem cells are segmented at the same time, so that the nanoscale artificial stem cell vesicles are obtained. Finally, the fresh stem cells are processed into the nanoscale cell vesicles with uniform size, the technological process is simple, the loss of the finally prepared stem cell vesicles to the total protein amount is about 26%-35%, the loss is less, and the protein types are not lost.