222 results about "Vesicle/vacuole" patented technology

The invention provides delivery systems comprised of stabilized multilamellar vesicles, as well as compositions, methods of synthesis, and methods of use thereof. The stabilized multilamellar vesicles may comprise prophylactic, therapeutic and/or diagnostic agents.

The invention relates to a medicament carrier and a preparation method thereof, in particular to a medicament delivery system comprising biodegradable polymer vesicles with asymmetric membranes. The invention discloses the biodegradable polymer vesicles and preparation and application thereof. The biodegradable polymer vesicles are prepared from A-B-C type block polymer, wherein a block A is polyethylene glycol (PEG) distributed on outer surfaces of the vesicles; a block B is hydrophobic biodegradable polymer to form the nucleuses of the vesicles; and a block C is polyelectrolyte distributed on inner walls of the vesicular membranes and used for efficiently loading medicaments with opposite electric charge. The biodegradable polymer vesicles are formed in aqueous solution directly, can efficiently load protein and polypeptide medicaments, nucleic acid medicaments and micro-molecular medicaments, and are expected to be applied to the protein therapy and the combination therapy of cancers.

Compositions and methods for delivering cargo to cells are provided. One aspect provides a synthetic vesicle containing caveolin 1 or a fragment thereof in an amount effective to form lipid rafts in the vesicle. The synthetic vesicles can be used to deliver polynucleotides, proteins, therapeutic agents, or a combination thereof to specific membrane-bound compartments of a cell. In certain aspects, the synthetic vesicles can deliver cargo to cellular organelles such as mitochondria.

The invention provides an anti-aging co-transport nano lipid vesicle and a preparation method and application thereof, and belongs to the technical field of functional cosmetics. The anti-aging co-transport nano lipid vesicle comprises the following components in parts by weight: 0.02-32 parts of an anti-aging active component, 11.1-65 parts of a nano lipid vesicle, and 3-88 parts of water, wherein the nano lipid vesicle comprises the following raw materials in parts by weight: 5.0-20.0 parts of a nonionic surfactant, 5.0-30.0 parts of water-soluble alcohol, 1.0-10.0 parts of compound phosphatide, and 0.1-5.0 parts of cholesterol; and the anti-aging active component includes a water insoluble anti-aging component and/ or a water soluble anti-aging component. According to the preparation method, the anti-aging active components are loaded in the same nano lipid vesicle, so that transdermal co-transport of multi-target-point anti-aging active components of various anti-aging mechanisms can be realized; and synergistic interaction is realized, and thus the anti-aging effect is improved.

Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.

The invention provides an indocyanine green composite nanoparticle which comprises indocyanine green, a hydrophobic polymer, polyethylene glycol derived phospholipid and a cancer cell membrane. The indocyanine green is encircled by the hydrophobic polymer and forms a sphere-like structure together with the hydrophobic polymer; the polyethylene glycol derived phospholipid penetrates the cancer cell membrane and forms a vesicle structure together with the cancer cell membrane; the sphere-like structure is wrapped in the vesicle structure. The invention further provides a preparation method and application of the indocyanine green composite nanoparticle. When the indocyanine green composite nanoparticle is applied, the indocyanine green composite nanoparticle has an efficient tumor targeting property, the indocyanine green can hardly gather and high stability is realized.

The invention provides a preparation method of a core-shell structured Fe3O4@MCM-41 magnetic nano material, and relates to a method for synthesizing Fe3O4 magnetic nano material from a vesicle phase. The preparation method of the core-shell structured Fe3O4@MCM-41 magnetic nano material aims to solve the problems of uneven core-shell structure and small specific surface area of the magnetic nano material. The preparation method comprises the following steps of: 1, preparing a vesicle phase solution from hexadecyl trimethyl ammonium bromide and sodium dodecyl benzene sulfonate; 2, dissolving FeCl3 and FeSO4 in secondary distilled water, and then adding the vesicle phase solution to the obtained iron salt solution, adding quadrol to adjust the pH value of the system after ultrasonic treatment to obtain a black suspension; and 3, adding hexadecyl trimethyl ammonium bromide and TEOS (Tetra Ethyl Ortho Silicate) to the black suspension, carrying out a crystallization reaction after pH adjustment, washing and drying the solid-phase crystals and then roasting the solid-phase crystals to obtain the magnetic nano material. The preparation method of the core-shell structured Fe3O4@MCM-41 magnetic nano material is characterized in that the vesicles formed by compounding an anionic surfactant with a cationic surfactant are taken as micro-reactors, the thermodynamic performance of the system is stable, and in the meantime, the specific surface area of the magnetic nano material is large.

The invention belongs to the field of high-polymer materials and discloses a high-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as a preparation method and an application of the high-polymer vesicle. The high-polymer vesicle is mainly formed through self-assembly of the AIE molecules and amphiphilic block copolymers; the outer layer and the inner layer of a high-polymer vesicle film are hydrophillic layers, each hydrophillic layer is formed by hydrophilic chain sections in the amphiphilic block copolymers, and a film intermediate layer between the outer layer and the inner layer is formed by hydrophobic chain sections in the amphiphilic block copolymers and the AIE molecules. The high-polymer vesicle has high fluorescence intensity and can meet different fluorescent staining demands. The preparation method is simple and feasible, the production efficiency is high, and the repeatability is good. The high-polymer vesicle can be used for related fields of optical bioimaging, biological detection and clinical imaging, high-polymer vesicle research and the like.

Disclosed is a vesicular system comprising a surface with a vesicle immobilized thereon. The immobilized vesicle has a circumferential membrane of an amphiphilic polymer. The vesicle is coupled to a surface by means of a molecule with a non-polar moiety. The non-polar moiety comprises a main chain of 3 to about 30 carbon atoms and 0 to about 12 heteroatoms selected from Si, O, S, and Se. The molecule with the non-polar moiety is coupled to the surface via a covalent or non-covalent bond. A portion of the non-polar moiety is integrated in the circumferential membrane.

Disclosed are methods, compositions, devices, and kits for the isolation of brain-specific exosomes. Specifically, methods, compositions, devices, and Unbound kits comprising an isolated brain-specific extracellular vesicle or exosome joined to a first binding agent that is specific for tau, β-amyloid, SlOO β, neuron-specific enolase, glycoprotein A2B5, CD133, NQ01, synaptophysin, neuronal nuclei, MAB 1569, polysialic acid-neural cell adhesion molecule (PSA-NCAM), or neurogenic differentiation 1 (NeuroD or Beta2), or glycosylated or phosphorylated forms of these molecules, are provided.

The invention belongs to the field of nano biological medical materials and particularly relates to polymer vesica with the surface deposited with antibacterial nano silver and a preparation method thereof. Amphipathic block polymers are self-assembled by a cosolvent method to form the vesica, and then the nano silver is generated in situ on the surface of the vesica. The polymer vesica has biocompatibility and has certain temperature and pH sensitivity; a cavity structure of the vesica can wrap various medicines; and simultaneously, due to the existence of the nano silver, the vesica also has the curative effects of sterilization and bactriostasis.

A hydrated lecithin carrier vesicle composition includes a lecithin-derived membrane-forming lipid vesicle in conditioned water for incorporation of an active ingredient to form a dispersed composition. A method of making the hydrated lecithin carrier vesicle includes using lecithin having not more than about 80% w/w phosphatidylcholine in the presence of conditioned water.

The invention belongs to the fields of high polymer chemistry and biomedical engineering, and particularly discloses a pH and temperature sensitive polymer. The polymer is composed of a hydrophilic polyethylene glycol segment, and a lyophobic poly-(aspartic acid-diethyl-ethylenediamine-co-histamine-co-diisopropyl ethylenediamine) segment, and the ratio of the hydrophilic segment to the lyophobic segment is (1:10)-(1:12). The pH and temperature sensitive polymer can be used for preparing nano-vesicles loaded with hydrophilic anti-tumor drugs or/and ultrasonic contrast agents, and the nano-vesicles can be used for preparing tumor diagnosis drugs or tumor treatment drugs.

The invention discloses a macrophage drug-loaded MP (microparticle) preparation and a preparation method thereof. The macrophage drug-loaded MP preparation comprises cell vesicles and drug small molecule effective components wrapped in the cell vesicles, wherein the cell vesicles are released by apoptosis of mannose modified macrophages. The drug-loaded microparticles can be highly enriched in tumor tissue and more easily absorbed by M2 type tumor-related macrophages, the antipolarization effect of small molecule drugs on M2 type tumor-related macrophages is improved, the tumor microenvironment is improved, killing of tumor cells is enhanced, the problems that small molecule drugs cannot be effectively enriched or target and antipolarize M2 type tumor-related macrophages at tumor sites canbe solved, and toxic and side effects of the small molecule drugs on organisms are also reduced.

A cosmetic or pharmaceutical rinse-off formulation contains 0.2% by weight to 20% by weight of a phase A; 0.1% by weight to 50% by weight of an active substance; a detergent; and the remainder being water. The phase A is a mixture of 20% by weight to 90% by weight of a surfactant mixture of isethionates, acyl lactylates and one of alkyl glutamates and alkyl glucosides and 10% by weight to 80% by weight of water, alcohol, polyol or mixtures thereof. The phase A forms multilamellar vesicles. The cosmetic or pharmaceutical rinse-off formulation is a clear product with the multilamellar vesicles of phase A having an average diameter of less than 100 nm.

Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.

The invention provides a method for preparing a multi-layer bio-based vesica capable of releasing insulin, and belongs to the field of functional materials. The method uses a bio-based macro-molecule as a main raw material, and comprises the following steps: respectively modifying sodium alginate (Alg) and chitosan oligosaccharide (CS) by function monomer 3-aminobenzeneboronic acid (APBA) and 3-carboxyphenylboronic acid (CPBA) to synthesize Alg-g-CPBA grafted copolymer and CS-g-CPBA grafted copolymer; preparing a multi-layer bio-based macromolecular microsphere by using insulin pre-embedded silicon dioxide nano-particles as a template by virtue of layer-by-layer self-assembly; adding CaCl2 to enable the Alg layer to perform physical crosslinking so as to improve the microsphere stability; and finally, removing the template to prepare the multi-layer bio-based vesica capable of releasing insulin. The prepared vesica has high stability, unique hollow structure and excellent biocompatibility, and has a covering amount on insulin being 60 percent; and controllable release of insulin can be realized under the stimulating action of glucose with different concentrations, and the release capacity is 80 percent. The vesica has a potential application value in the field of drug loading, controllable release and the like.

Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.

The invention discloses a polypeptide combination method for wrinkle reduction. Through one or more polypeptides, the fusion of vesicles and a cell membrane is inhibited so that release of acetyl choline is inhibited. The polypeptide combination method utilizes a wrinkle reduction polypeptide composition with a mass concentration of 0.001% to 5%, a humectant with a mass concentration of 0.001%-10%, an antibacterial stabilizer with a mass concentration of 0.001%-2%, a preservative with a mass concentration of 0.001%-2% and a pH adjuster. The wrinkle reduction polypeptide composition comprises one or more of acetyl hexapeptide-8, palmitoyl pentapeptide-4, venom-like peptide and copper tripeptide-1 and the balance of deionized water and has a mass concentration of 0.001%-5%. According to thewrinkle production mechanism and different polypeptide action targets, the invention provides the wrinkle reduction polypeptide composition.

The invention relates to an intravenous oncolytic virus preparation and a preparation method thereof. The method performs transfection of a targeting polypeptide gene to a eukaryotic cell, and displays the targeting polypeptide on a cell membrane to obtain the gene engineering reconstructed cell strain capable of stably expressing the targeting polypeptide, The cell strain is used to extract nano-sized cell membrane vesicles with a uniform size, and then the oncolytic virus (OV) is loaded into the cell membrane vesicles with engineering reconstruction to synthesize an intravenous oncolytic virus complex which can be targeted for tumor delivery. The intravenous oncolytic virus preparation encapsulates the oncolytic virus in the cell membrane vesicles, and can effectively reduce the neutralizing effect of a neutralizing antibody on the oncolytic virus in the body, the cell membrane vesicle has targeting performance after gene engineering reconstruction, can achieve targeted delivery of the oncolytic virus and enrichment at the tumor site, and reduces the systemic side effects of the oncolytic virus.

The invention relates to a preparation method of a nonionic vesicle with a whitening effect. The preparation method is characterized by comprising following steps: step A, weighing a conventional whitening agent, hydroxyl phenoxyl propionic acid, phloretin, hydroxytyrosol, and a cyanotis arachoidea root extract according to a mass ratio of 3-10:1:3:2:1, and then mixing the mixture with polyol to obtain a whitening active composition solution; step B, weighing glyceryl oleate citrate, cetearyl glucoside, and polyglyceryl-3 methyl glucose distearate according to a mass ratio of 0.5-3:2:1, and adding magnolol to obtain a nonionic surfactant premix; step C, preparing a nonionic surfactant water solution; and step D, mixing the whitening active composition solution with the nonionic surfactant water solution according to a mass ratio of 1:2-4 to prepare the nonionic vesicle with a whitening effect. The vesicle can wrap multiple whitening active components and overcomes the problems such as single whitening mechanism, bad product stability, low whitening efficiency, easy triggering of skin irritation, and the like.