Aquaporin based thin film composite membranes

An aquaporin and composite membrane technology, applied in the field of manufacturing the thin film composite membrane, can solve problems such as obstacles

Active Publication Date: 2014-11-12
NANYANG TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, high energy consumption due to limited hydraulic permeability remains an obstacle for the use of TFC membranes for water filtration purposes including desalination, see Elimelech et al., The Future of Seawater Desalination: Energy, Technology, and the Environment; Science333,712 (2011)

Method used

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  • Aquaporin based thin film composite membranes
  • Aquaporin based thin film composite membranes
  • Aquaporin based thin film composite membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1. prepare liposome and proteoliposome

[0062] Materials and methods used for preparation

[0063] Unless mentioned otherwise, ultrapure water with a resistivity of 18.2 MΩcm from a Milli-Q ultrapure water system (Milli-pore Singapore Pte Ltd) was used to prepare the reagents in this study. Analytical grade NaCl, KCl, KH with a purity greater than 99% 2 PO 4 , MgCl 2 , MgSO 4 and Na 2 SO 4 Available from Merck (Germany). Sucrose (ultrapure grade) was obtained from USB Corporation (Cleveland, USA). Chemicals used in aquaporin Z expression and purification (including Ampicilin, Chloramphenicol, IPTG, Tris, β-mercaptoethanol, glycerol, and lysozyme) were obtained from Sigma-Aldrich and purchased from ACS (American Chemical Society) grade or SigmaUltra grade. Benzonase was purchased from Merck. Ni-NTA resin was purchased from Bio-Rad. n-Octyl-b-D-glucopyranoside (OG, ultrapure grade, Merck, Germany) was used as a detergent during proteoliposome prepar...

Embodiment 2

[0097] Example 2. Preparation of thin film composite membranes with lipid-AqpZ vesicles and testing in RO devices

[0098] A commercial UF membrane (MWCO, 50,000 Dalton (Dalton)) was used as the substrate, 50 ml of amine aqueous solution 1.5 wt% MPD containing 0.08 mg / g DOPC-AqpZ vesicles was spread on the surface of the UF membrane substrate, and the substrate was held with the aqueous solution Wet for 15 minutes. Subsequently, the aqueous amine solution was removed from the surface, and the substrate was allowed to stand vertically in air for 10 minutes, then the surface was blown with compressed nitrogen gas at 2 bar for 1 minute to remove any possible aggregated vesicles on the surface, and then the substrate was allowed to continue Dry vertically for another 20 minutes. Subsequently, a 0.1 w / v% TMC solution was poured on the surface layer of the saturated substrate and allowed to react for 1 minute. The resulting membranes were stored in Milli-Q water until use. The tf...

Embodiment 3

[0099] Example 3. Preparation and thin film composite membrane with lipid vesicles and tested in RO device

[0100] The two stages of interfacial polymerization and the composition of the reactive monomer solution during the process were similar to Example 2, except that only 0.08 mg / g DOPC vesicles without AqpZ were dissolved in the aqueous amine solution. RO test was carried out as in Example 2. The flux and rejection rate of the thin film composite membrane with DOPC-AqpZ vesicles to 500ppm NaCl (200 psi) are 16.9L / m2.h and 98.5%, respectively. Results from experiments involving comparisons of water flux and solute rejection for thin-film composite membranes incorporating lipid vesicles only or lipid-AqpZ vesicles at increasing pressures up to 200 psi are shown in Figure 7 , which clearly shows that a relatively high water flux can be obtained with the incorporation of DOPC vesicles. However, incorporation of DOPC-AqpZ vesicles significantly improved the water flux of th...

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Abstract

Present invention relates to a thin film composite membrane wherein a thin selective layer, having incorporated amphiphilic vesicles, is supported by a microporous substrate. A process of preparing the thin film composite membrane and its use are also disclosed.

Description

technical field [0001] The present invention relates to thin film composite membranes in which aquaporin water channels have been incorporated into the active layer. Furthermore, the present invention relates to a method of manufacturing the thin film composite membrane and its use in filtration processes such as nanofiltration and osmosis filtration processes. Background technique [0002] With the increasing shortage of fresh water and energy sources worldwide, more and more attention is paid to the desalination of seawater and brackish water. Using many techniques, such as multi-effect distillation (multi-effect distillation, MED), multi-stage flash (multistage flash, MSF) and vapor compression and membrane desalination (such as reverse osmosis (reverse osmosis, RO) or nanofiltration (nanofiltration, NF)) [1]. [0003] The most efficient way for biological membranes to exhibit water transport properties across osmotic pressure gradients is via the aquaporin (AQP) protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D69/12B01D71/80B01D67/00B01D69/14
CPCB01D67/0006B01D69/12B01D71/80B01D71/68B01D69/144B01D2323/40B01D69/125B01D61/00B01D61/027B01D61/025B01D71/56B01D71/70B01D61/002
Inventor 汤初阳邱长泉赵阳沈文明亚查拉朋·维拉瓦塔纳维屈王蓉胡晓杰米·托瑞斯安东尼·G·范恩克劳斯·海力克斯-尼尔森
Owner NANYANG TECH UNIV
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