322 results about "Chloramphenicol" patented technology

The invention relates to a method for analyzing (1R, 2R)-2-amino-1-(4-(methylsulfonyl)-phenyl)-1,3-propylene glycol as an important intermediate of florfenicol, which is chloramphenicol spectrum antibiotic special for animals. The method comprises the following steps of: performing a reaction of methylsulfonyl benzaldehyde and nitro alcohol at 0-40 DEG C in the presence of a chiral catalyst to obtain (1R, 2R)-2-nitro-1-(4-(methylsulfonyl)-phenyl)-1,3-propylene glycol; and reducing the (1R, 2R)-2-nitro-1-(4-(methylsulfonyl)-phenyl)-1,3-propylene glycol through hydrogen to obtain the intermediate. In the invention, the compound (1R, 2R)-2-amino-1-(4-(methylsulfonyl)-phenyl)-1,3-propylene glycol with a high e.e. value is obtained by adopting the chiral catalyst, and the compound has the advantages of strong reaction selectivity, high productivity, simple process, low cost, easy preparation of raw materials and low price, is suitable for industrial production, avoids chiral separation frequently used in industry at present and saves the raw materials and the cost. The compound (1R, 2R)-2-amino-1-(4-(methylsulfonyl)-phenyl)-1,3-propylene glycol is used for preparing florfenicol and can reduce the preparation cost.

A detection method of a nitrofuran metabolites and chloramphenicol multiresidue in shrimp belongs to the technical field of aquatic products detection. The method is as below: hydrolyzing a sample with hydrochloric acid, subjecting nitrofuran metabolites to derivatization by using 2-nitrobenzaldehyde, adjusting the pH value to 6.5-7.5, adding acetonitrile, adding an extraction salt package; conducting liquid-liquid extraction of a target compound by using acetonitrile; purifying and concentrating a supernatant; then determining by a liquid chromatography-tandem mass spectrometer, and quantifying by an internal standard method. The method uses a novel sample extraction and purification mode for simultaneous analysis and determination of two prohibited drugs with the highest detection rate in shrimp, overcomes the limitations of separate determination of two drugs in the detection method of the prior art, improves the extraction efficiency of chloramphenicol in actual positive samples, greatly improves the work efficiency, shortens the work time and saves reagent consumption and labor costs. The method adopts the isotope internal standard method for quantification, the determination result is more accurate and reliable, has high sensitivity, and good reproducibility and quantitative accuracy.

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

The invention relates to a method for detecting antibiotic residues in milk, and an application thereof, and belongs to the field of food safety. The invention discloses an online solid phase extraction-high performance liquid chromatography-mass spectrometry detection method in order to solve the detection problem of antibiotic residues in milk. The method allows five types of 33 veterinary drug residues comprising sulfanilamides, quinolones, beta-lactams, tetracyclines and chloramphenicols in the milk to be simultaneously detected. Influences of various factors on the reservation and selectivity of various antibiotics are optimized, so the method has the characteristics of high sensitivity, high recovery rate, high stability and high precision. The detected antibiotics can be qualitatively and quantitatively determined according to the reservation time and accurate mass numbers. The amounts of samples and reagents required and used in the method are small, the detection speed is fast, all operation processes are completed within 15.5min, and cost reduction and operating risk mitigation of dairy product processing enterprises are guaranteed.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention relates to a silk screen printing electrode and a method for synthesizing chloramphenicol molecularly imprinted membrane, belonging to the macromolecule technical field. The invention embeds template molecules in the direct surface in-situ polymerization of a one-time silk screen printing electrode and elutes the template molecules, to form the cavities of fixed sizes and shapes and the function groups of a fixed arrangement in the molecularly imprinted membrane, therefore, the molecularly imprinted membrane has shape memory property to the stereo structure of the template molecules, the chloramphenicol molecule of the object sample can be combined with the cavity specifically, to modify the electromechanical property of the molecularly imprinted membrane to improve the electric conductivity of the molecularly imprinted membrane and improve membrane passing current. The silk screen printing electrode coated with a chloramphenicol molecularly imprinted membrane can be connected with a current sensor to quickly and sensitively detect chloramphenicol density. The molecularly imprinted membrane can be used to detect chloramphenicol, having simple sample pretreatment, short detection time, strong interference resistance and batch production.

This invention relates to a method of treating otitis externa using a topical combination medication, including one or more antifungal agent such as, for example fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, caspofungin, micafungin, terbinafine, naftifine, butenafine, amorolfine, ravuconazole, posaconazole, flucytosine, econazole, enilaconazole, miconazole, oxiconazole, saperconazole, sulconazole, terconazole, tioconazole, nikkomycin Z, anidulafungin (LY303366), nystatin, pimaricin, griseofulvin, ciclopirox, haloprogin, tolnaftate, and undecylenate and one or more antibacterial agent such as neomycin sulfate, polymyxin B sulfate, colistin sulfate, gentamycin, tobramycin, chloramphenicol, Ciprofloxacin, Ofloxacin, a penicillin compound, a cephalosporin compound, a macrolide compound, a fluoroquinolone compound, streptomycin, or kanamycin.

The invention relates to a preparation method of a chloramphenicol molecular imprinted adsorbing material on the surface of a magnetic carbon microsphere. The magnetic carbon microsphere is prepared from shaddock peel by adopting a solvent thermal synthesis method, the surface of the magnetic carbon microsphere is subjected to ethylene functional modification by KH570, the treated magnetic carbon microsphere is used as a matrix material, chloramphenicol is used as a template, hydroxyethyl methylacrylate and 4-vinylpyridine are used as functional monomers, N-isopropyl acrylamide is used as a thermosensitive monomer, ethylene glycol dimethacrylate and N,N-methylene bisacrylamide are used as cross-linking agents, 2,2'-azobis[2-methylpropionamidine] dihydrochloride is used as an initiator, and the magnetic carbon microsphere surface imprinted thermosensitive adsorbing material is prepared in a methanol / water mixed system, and the adsorbing material is used for selective recognition and selective separation of chloramphenicol in a water environment. The prepared molecular imprinted adsorbing material on the surface of the magnetic carbon microsphere is low in cost, can be prepared easily, has the high recognition and selectivity properties, high separation and enrichment ability and high adsorption rate of target molecules, and is high in adsorption speed.

The invention relates to a method for preparing chloramphenicol affinant column and its usage, which adopts protein A -Sepharose 4 fast flow as phase carrier, the protein A of the carrier is coupled with the chloramphenicol antibody; the coupled chloramphenicol antibody is bonded with the fixed phase chemistry; the affinant column can suit for many stroma; it uses protein A so that IgG can be connected with it with high desirability and large load capacity of the chloramphenicol column.

The invention discloses a feed special for a low-fat meat duck without antibiotic and fishy smell, which consists of the following components by weight percentage: 10-25 of lactobacillus fermented material and 75-90 of main material, wherein the lactobacillus fermented material consists of the following raw materials: cotton dregs, rapeseed dregs, bran, peanut meal, maize and lactobacillus fermented liquid; and the main material consists of the following raw materials: bean dregs, maize protein powder, orexin and growth promotion agents, de-fishy agents, stone dust, calcium hydrophosphate, trace elements and maize flour. 10-25 percent of the obtained lactobacillus fermented material is taken according to the percentage of the total weight of the feed for bagging, and 75-90 percent of the obtained main material is taken according to the percentage of the total weight of the feed for packing after bagging, thus the feed special for the meat duck is prepared. The duck fed by the feed which is prepared by uniformly mixing the lactobacillus fermented material and the main material has pork type meat quality, abundant muscles, less fat and high protein content, is inspected to have no chloromycetin, terramycin, aureomycin and sulfanilamide medicaments by the national meat product supervision and inspection center and completely reaches a non-antibiotic standard and meets the requirement of food security of the European Union.

The present invention relates to a preparation method of chloromycetin molecular engram polymer microsphere, belonging to field of biological engineering technology. Its preparation method includes the following concrete steps: the mole ratio of template molecule, functional monomer and ethylene glycol dimethacrylate is 1:2-8:25; volume ratio of total volume of diluting agent and ethylene glycol dimethacrylate and water is 1:6-14; dissolving polyvinyl alcohol 1788 in water, dissolving functional monomer and template molecule in diluting agent; mixing ethylene glycol dimethacrylate, azobisisobutyronitrile and diluting agent; mixing the above-mentioned three solutions, after the reaction is completed, stirring product in water, cooling and filtering, washing successively by using double-distilled water, methyl alcohol and acetone, utilizing Soxhletí»s extraction process to remove template molecule; vacuum drying so as to obtain the invented chloromycetin molecular engram polymer microsphere.

The invention relates to an artificial feed of a rice leaf folder and a preparation method of the artificial feed. The artificial feed belongs to the technical field of insect raising. The formula is prepared from the following components of water, rice leaf powder, wheat germ powder, corn meal, soybean meal, casein, granulated sugar, agar, yeast, mildew inhibitor, antioxidant, insect feed oil, antibiotic, choline chloride, wade-salt, cholesterol and vitamine. The mildew inhibitor used in the formula is composed of 2-3 of natamycin, sorbic acid, nipagin, tebuconazole and azoxystrobin; the antioxidant is mixture of ascorbic acid and phospholipid; the insect feed oil is the mixture of linseed oil, rice bran oil and germ oil, and the antibiotic may be streptomycin or chloramphenicol or aureomycin. Survival rates of larvas can be obviously improved by raising the rice leaf folder by the feed in the formula, and a foundation is created for an indoor research of the rice leaf folder.

The invention discloses a fashi citric acid rod Citrobacter farmeri SC01 and the application. The bacterium is obtained by artificial accumulation culture and separation cleansing from phenols cyanogen waste water treatment station one level aerobic pool sludge. The bacterium is preserved in china typical culture entreasure center of Wuhan University on January 7th 2008 year whose short form is CCTCC and preserving identification serial number is CCTCC NO: M208004. The fashi citric acid rod Citrobacter farmeri SC01 incorporating the invention can degrade phenyl hydrate, 2-methylphenol, m-cresol, 1-naphthyl hydroxide, 696mg / L m-cresol in 114 hours on aerobic condition, and the average degrading rate of speed ran up to 8.94mg / (L h) and the bacterium is sensitive to acheomycin, chloramphenicol, rifampicin and streptomycin, and the bacterium can be used safe and wide in harnessing and repairing wasted water and soil phenols pollution because the bacterium can not carryover drug-resistance factor spreading in aborigines bacterium when the bacterium is released into environment.

The invention discloses a berberine azole compound shown in general formulas such as I-IV, and a pharmaceutically acceptable salt thereof, and also discloses a preparation method of the compound, which comprises the following steps that: a compound VI and an azole compound HIm or 1H-2-mercaptobenzimidazole are reacted to obtain an intermediate VII or VIII, and then the obtained intermediate is reacted with a compound V to obtain the berberine azole compound shown in general formulas such as I or II; and the compound V and a compound IX or XI are reacted to obtain an intermediate X or XII, and then the obtained intermediate is reacted with the azole compound Him to obtain the berberine azole compound shown in general formulas such as III or IV. The berberine azole compound has a certain inhibitory activity on Gram-positive bacteria, Gram-negative bacteria and fungi, the anti-bacterial activity of part of the compounds is equal to or even stronger than that of chloramphenicol or norfloxacin, the antifungal activity of part of the compounds is equal to or even stronger than that of fluconazole, and the berberine azole compound can be used for preparing anti-microbial drugs.

The invention is a kind of immune colloidal gold reagent for measuring the oxymycin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of oxymycin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by oxymycin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

The invention relates to a paper chip for detecting the residual antibiotic in milk, particularly to a paper chip detection method for the residues of five antibiotics such as tetracycline, chloramphenicol, sulfanilamide, quinolone and beta-lactam in milk. A purpose of the present invention is to provide a multi-channel detection paper chip. According to the present invention, the detection quantity, the detection index and the detection time are improved, the opening platform is provided, and the detection index on the paper chip can be adjusted according to the actual demand; and the results show that the paper chip has advantages of low cost, high sensitivity, platform opening, accurate result and the like, and provides a certain guidance significance for the development of the paper chip in the food safety detection field.

A method for preparing test paper of chloromycetin colloidal gold detection technique includes anchoring chloromycetin single resistance being labeled with colloidal gold and chloromycetin coupled antigen as well as resisting mouse IgG antibody on nitrocellulose film and on base plate, using chloromycetin coupled antigen as detectionbelt and using resisting mouse IgG antibody as reference belt .The prepared test paper can detect out residual amount of chloromycetin in serum sample or in urine sample quickly and accurately.

The invention belongs to the technical field of bioengineering, and discloses a method for improving the alcohol tolerance of yeast by utilizing lactic acid bacteria. The method includes: adding a lactic acid bacteria exogenously into a yeast culture system for mixed culture for 4h to obtain normal saline with resuspended bacteria and an alcohol content of 8% (v / v), and performing stressing undera 30DEG C condition for 90min; centrifugally washing the bacteria subjected to alcohol stress, then dibbling the bacteria onto a yeast extract peptone glucose (YPD) agar culture medium containing 0.01% chloramphenicol, performing standing and culturing at 30DEG C for 36h, then counting bacterial colonies, and calculating the survival rate. Results show that after alcohol stress, the survival rateof the yeast obtained by mixed culture after lactic acid bacteria is added is 23.64%, the survival rate of yeast obtained after pure culture is 3.87%, and the survival rate is increased by 6.11 times.The method is simple and convenient to operate, and can be used for improving the alcohol resistance of yeast.

The invention discloses a preparation method of a chloramphenicol-containing fishmeal standard material. The method is characterized in that the raw fishmeal polluted by chloramphenicol is obtained through simulating feeding; a sample is preprocessed and mixed with water by proper ratio; then the mixture is uniformly pulped; the freeze-dried fish is obtained by a freezing drying method; the freeze-dried fish is grinded, screened, uniformly mixed and irradiated to prepare the freeze-dried fishmeal which is high in uniformity; the uniformity is detected, the stability is monitored, and the standard material is assigned by high-accuracy measuring through a liquid chromatography-isotope dilution mass spectrometry. The trace chloramphenicol containing freeze-dried fishmeal is high in uniformity and stability and is applicable to the evaluation of method for analyzing the trace chloramphenicol residue in an aquatic product, the quality control in analyzing, and the assessment of lab ability; the standard material is applicable to calibrating of metering devices and evaluation of measurement methods.

The invention provides a gold nano-cluster as well as a preparation method and an application thereof. The preparation method comprises the following steps: mixing a xylanase solution with an HAuCl4 solution, and sufficiently and uniformly mixing for 5-10 minutes by using a vortex device; adding a NaOH solution to adjust the pH value to be 8-13, and uniformly mixing for 5-10 minutes again by usingthe vortex device; and reacting in a water bath kettle in a dark place for 6-24 hours to obtain the product. The gold nanocluster is sensitive to Hg<2+> response, a detection system is easy and convenient to operate, controllable and short in consumed time, and the gold nanocluster can be applied to detection of the content of Hg<2+> in environmental water samples and soil samples. Meanwhile, theinvention also provides a method for detecting chloramphenicol by using a fluorescence method, and the gold nano-cluster fluorescent probe is combined with a standard curve, so that the detection process is simple, convenient and quick, the detection cost is low, and the method can be used for detecting the content of chloramphenicol in milk and meat. The method has the advantages of low detection limit, wide detection range, high sensitivity and the like, and can provide a new thought for detecting the content of chloramphenicol in animal food.

The invention discloses a method for treating chloramphenicol wastewater by electrochemical reduction oxidization and a reactor of the method. The method comprises the following steps: introducing thechloramphenicol wastewater into a cathode chamber of a reactor main body to fully fill the whole reactor main body; carrying out magnetic stirring to start electrochemical treatment; enabling the wastewater to be subjected to reduction reaction in the cathode chamber; conveying the wastewater treated by the cathode chamber into an anode chamber through a connecting pipeline between the cathode chamber and the anode chamber; enabling the wastewater to be subjected to oxidization reaction in the anode chamber, wherein the current density of the oxidization reaction is 10 to 30mA / cm<2>, the staying time of the wastewater in the cathode chamber and the anode chamber is 1 to 2h and the reaction temperature is 10 to 30 DEG C. According to the method disclosed by the invention, the disadvantagesof a single oxidization method that the treatment efficiency on the chloramphenicol wastewater is low and the toxicity of the treated wastewater is high are overcome through a reduction oxidization method; the concentration of the chloramphenicol wastewater can be efficiently reduced through the reactor and the method, the toxicity of the wastewater is reduced and the biodegradability of the wastewater is improved.

The invention discloses a magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method. Chloramphenicol aptamer modified magnetic nanoparticles are combined with chloramphenicol in a sample, rapid separation and enrichment are achieved through magnetic separation operation, one section of capture probes complementary with chloramphenicol aptamer sequence portions are modified on the surface of a gold electrode and used for combining aptamer loci without being occupied by the chloramphenicol, the magnetic nanoparticles are marked, markers of the magnetic nanoparticles are converted into Prussian blue with electrochemical activity by the aid of an electrochemical method, and output signals inversely proportional to the concentration of the chloramphenicol are acquired according to current of electrochemical reduction peaks. According to the sensor, magnetic separation, magnetic enrichment and signal amplification are integrated, time and reagent cost are saved, detection steps and use processes of experimental instruments are simplified, the sensor is rapid, low in cost and high in operability, and sensitive detection of the chloramphenicol is achieved.

The invention belongs to the technical field of food safety, and particularly relates to a pretreatment method for detecting chloromycetin in an animal product. The pretreatment method disclosed by the invention comprises the following operations: weighing of a to-be-detected sample, extraction, cyclotron oscillation, centrifugation, purification, derivatization and the like, wherein the used apparatus comprises a centrifugal machine, an oscillator, a constant-temperature drying oven, a pressure blowing concentrator and the like. A sample solution prepared by using the pretreatment method can be directly detected by virtue of a gas chromatography-mass spectrometry method. Compared with an existing method, the pretreatment method has the advantages of being low in operation cost, high in repeatability, high in accuracy, high in sensitivity and the like, has strong operability, is expected to be popularized and applied on a large scale in the industries such as agriculture, quality inspection departments, and entry and exit inspection and quarantine, and has significant economic benefits. According to the pretreatment method, whether chloromycetin is contained in meat can be accurately detected, and the detected animal product is safe to eat and accords with the requirements of the national standard in China.

The invention discloses a chloramphenicol molecular imprinting polymer microballoon which has mean grain size of 1mum to 300mum and grain size distribution coefficient C.V of not more than 15 percent. The preparation method of the chloramphenicol molecular imprinting polymer microballoon comprises the following steps of: firstly, preparing emulsion by using a micro-fluidic device; secondly, curing the emulsion to obtain a molecular imprinting microballoon, wherein the micro-fluidic device comprises a plurality of micro channels; generating the emulsion when a dispersion phase is driven by an injection pump to enter a continuous phase from the micro channels; transferring the emulsion to another container and curing to obtain a polymer microballon; eluting the polymer microballon to remove a template molecule; and obtaining the molecular imprinting polymer microballoon. The dispersion phase contains the template molecule of chloramphenicol, a functional monomer, a cross linker, an evocating agent and a pore-foaming agent; and the continuous phase contains water and a dispersion agent. The invention also provides application of the chloramphenicol molecular imprinting microballoon.

The invention provides a high-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat. The method comprises a step of establishing a chemical pollutant standard quality spectrum library, a sample detection step and a screening analysis step. The method covers 152 target compounds such as sulfanilamides, quinolones, quinoxalines, nitroimidazoles, beta-lactams, tetracyclines, macrolides, chloramphenicols, benzimidazoles, nicotinic agonists, beta-receptor agonists, hormones and the like. The method has the advantages that the sample pretreatment method is simple and convenient; the 152 compounds can be screened and confirmed by one-needle sample injection; the detection flux is large; the accuracy is high; and the method is suitable for simultaneous qualitative confirmation detection of various veterinary drug residues in the livestock and poultry meat.

A monoclonal antibody GA3 of chloromycetin for mouse is disclosed, which has strong specificity for binding with chloromycetin. The immunoglobulin 6A3, its fragment and immune conjugate, and a reagent kit for detecting the residue of chloromycetin are also disclosed.

The invention relates to an effective Ustilaginoidea virens separation method, and belongs to the technical field of microbe. The method comprises the steps of: scraping a small amount of chlamydospore powder on surfaces of false smut balls with a pricking needle; placing the powder in a sterile petri dish; adding 0.5mL of sterile water suspension chlamydospores; then diluting the chlamydospore suspension by 2-3 gradients, with the ratio of the chlamydospore suspension to sterile water being 1:5 in a 1.8mL Eppendorf tube through a gradient dilution method; transferring 0.2mL of the diluted chlamydospore suspension to surface of a Wakimoto toceshi solid culture medium for separation (containing 50 mug / ml chloramphenicol); smearing evenly; inversely placing the petri dish into an incubator at 28 DEG C for dark culture; and effectively separating out target pathogens six days later. The method provided by the invention obviously weakens interference effect of infectious microbe in chlamydospores of rice false smut balls, has very simple operation procedure, is suitable for separation of rice false smut balls stored at normal temperature or 4 DEG C in a refrigerator within 3 months, and has the best separation effect on rice false smut ball bacteria just acquired in the field.

The invention relates to a Pediococcus acidilactici strain which is named Pediococcus acidilactici AAF 3-3. The Pediococcus acidilactici AAF 3-3 is classified as Pediococcus acidilactici, and is preserved under the preservation number of CGMCC No. 17856 since the preservation date of May 27, 2019 in the preservation unit of China General Microbiological Culture Collection Center (No.3, Court 1, West Beichen Road, Chaoyang District, Beijing). The Pediococcus acidilactici AAF 3-3 disclosed by the invention has high tolerance to main stress pressures (acetic acid, lactic acid, high temperature and ethanol) and an extreme pH value during solid-state fermentation of edible vinegar, and has an L-lactic acid production function; and moreover, the Pediococcus acidilactici AAF 3-3 has significant inhibitory effects on escherichia coli, micrococcus luteus and staphylococcus aureus as well as resistance to gentamicin, chloramphenicol, erythromycin, ampicillin and kanamycin. Thus, the Pediococcusacidilactici AAF 3-3 has application potential in the field of foods.

The present invention discloses one kind of feed additive for preventing bird flu and its preparation process. The feed additive consists of colocynth extract containing colocynth total alkaloid in 70-90 % and Chinese soapberry extract containing Chinese soapberry total alkaloid in 60-80 % in the weight ratio of 0.7-1 to 0.5-1. The colocynth extract and the Chinese soapberry extract are extracted from the above-ground parts of colocynth and Chinese soapberry separately. The additive is added into feed for raising fowls to prevent bird flu virus and has no latent side effect on human body caused by eating fowl products.

An analytical method for chloromycetin and 1,2-dihydroxypropane in the medicine- skin care products comprising, performing enrichment for the chloromycetin and 1,2-dihydroxypropane, conducting qualitative analysis to the halomycetin rich didymium using highly effective liquid phase color spectrum, elementary analysis, mass spectrometric analysis, infrared spectrum analysis, and inorganic metallicion determination, then conducting qualitative analysis to the 1,2-dihydroxypropane rich didymium using highly effective liquid phase color spectrum, infrared spectrum analysis, and inorganic metallic ion determination, last using color spectrum outer marking method to accomplish the quantitative analysis of the halomycetin and 1,2-dihydroxypropane. The invention solves the problem of concentration enrichment for the unknown compositions, and can be used widely.