98 results about "Chloramphenicol" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The invention discloses a strain of Chryseobacterium sp. For degrading chloramphenicol and application thereof. Belongs to the technical field of biochemical environment treatment. The chloramphenicol degrading bacterium provided by the invention is identified as Chryseobacterium sp. Through 16S rDNA (ribosomal deoxyribonucleic acid), the strain is preserved in the China Center for Type Culture Collection on November 26, 2024, and the preservation number is CCTCC NO: M 20242642. According to the efficient chloramphenicol degrading bacterium, the degradation rate of a degrading bacterium agent prepared from the efficient chloramphenicol degrading bacterium on chloramphenicol with the initial concentration of 100 mg / L within 48 h is as high as 96%. The chloramphenicol degrading bacterial agent is suitable for treating industrial wastewater, pharmaceutical wastewater, fishpond culture wastewater and other wastewater containing chloramphenicol pollution, the degrading effect is remarkable, the speed is high, secondary pollution can be avoided, and an efficient, economical and environment-friendly technical means is provided for solving the problem of antibiotic pollutant treatment.

The invention discloses a pseudomonas capable of efficiently and rapidly degrading chloramphenicol and application of the pseudomonas. The name of the strain is Pseudomonas sp. MH-W1, the preservation number of the strain is GDMCC No: 66907, and the strain is preserved in the Guangdong Microbial Culture Collection Center on September 1, 2025. The strain can tolerate 200 mg / L of chloramphenicol; 100 mg / L of chloramphenicol can be degraded within 8 h, and the degradation rate reaches about 80%. According to the method provided by the invention, the degradation time can be remarkably shortened, the degradation efficiency is improved, the degradation condition is mild, the operation is simple and convenient, large-scale application is easy to realize, and the method has a wide industrial popularization prospect. The strain is an aerobic bacterium, is fast in growth and reproduction, simple to prepare, low in cost, convenient to use and high in efficiency, and can be used for repairing the environment polluted by chloramphenicol.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The invention discloses a method for strengthening three-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in rice field soil through sulfurized zero-valent iron, and belongs to the technical field of rice field soil pollution remediation. The sulfurized zero-valent iron (S-ZVI) is prepared according to the sulfur-iron ratio of 0.1-0.2; the S-ZVI is mixed with the contaminated soil, and rice is cultivated in cooperation with a specific fertilization and irrigation scheme. According to the method for strengthening the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in the rice field soil through the sulfurized zero-valent iron, by optimizing preparation and application of the sulfurized zero-valent iron, under the synergistic effect of chemical reduction and microbial degradation, the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is expected to be achieved, and the effect of strengthening the rice field soil chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is achieved. And an important means is provided for degradation of antibiotics in rice field soil.

The invention discloses a rapid selective detection and monitoring culture medium for candida auricula as well as a preparation method and application of the rapid selective detection and monitoring culture medium, and belongs to the technical field of microbiological detection. The culture medium comprises the following components: peptone, yeast extract powder, glucose, agar, a selective inhibitor, a chromogenic substrate and auxiliary components, the chromogenic substrate comprises the following components: dulcitol and X-Gal, the culture medium disclosed by the invention is added with chloramphenicol and ciclopirox olamine to inhibit infectious microbes, and a chromogenic system of dulcitol and X-Gal realizes specific detection of candida auricula. The culture medium has the advantages of high selectivity, rapid identification and low cost, and is suitable for clinical sample and environment monitoring.

The invention discloses a COF-coated BDTP-coated Fe3O4 nano material as well as a synthesis method and application thereof. The nano material is obtained by wrapping the surface of aminated nanoscale Fe3O4 with a covalent organic framework material with high porosity and high crystallinity during synthesis, the nano material can effectively adsorb chloramphenicol, the maximum adsorption saturation of every 10 mg of the nano material to chloramphenicol is 9.89-10.4 mu g / mL, and the adsorption repeatability is good. Based on the obtained nano material, the invention also provides a pretreatment method for detecting chloramphenicol by using high performance liquid chromatography. Compared with a traditional pretreatment method, the pretreatment method has the advantages that the use amount of organic reagents is greatly reduced, the pretreatment cost is reduced, the pretreatment time is shortened, and the pretreatment method has good applicability, stability and feasibility, is beneficial to accurate detection of residual chloramphenicol in agricultural and sideline products and guarantees food safety.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The present invention relates to medicine and the pharmaceutical industry, and more particularly to agents capable of inhibiting inflammatory processes, and even more particularly to agents that have an antimicrobial, sorptive, angioprotective and wound-healing effect on local inflammatory processes of the skin and soft tissues, for use in surgery, traumatology and dermatology. What is proposed in the present invention is a combination including dexpanthenol, methyluracil and chloramphenicol, and also a pharmaceutical composition based thereon.

The invention discloses a chloramphenicol hapten, antigen, antibody and colloidal gold detection device as well as a preparation method and application thereof. According to the chloramphenicol hapten prepared by using a total synthesis method, all potential recognition sites of chloramphenicol are reserved to the maximum extent, particularly, the most critical dichloro structure of chloramphenicol is reserved for the first time, after a spacer arm is introduced, the distribution of electron cloud of the hapten is almost consistent with that of overall electron cloud of chloramphenicol, and the immunogenicity of the chloramphenicol antigen is improved; the chloramphenicol antigen and the monoclonal antibody prepared by the invention are high in specificity when being used for ELISA detection, the IC50 value is 8.21 pg / mL, the linear detection range is 1.71-39.45 pg / mL, and the cross reaction rates of the chloramphenicol antigen and the monoclonal antibody to common structural analogues are lower than 0.01%; and the sensitivity to chloramphenicol in a sample is 0.1 g / kg.

The invention relates to a method for electrochemically detecting chloramphenicol by using molecular imprinting. The method is characterized in that a chloramphenicol molecularly imprinted polymer prepared from a temperature-sensitive monomer N-isopropylacrylamide and Dawson type tungsten phosphate (P2W18) are jointly modified on a substrate electrode through a simple and controllable electrostatic layer-by-layer self-assembly method. The modified electrode synthesized by the method disclosed by the invention is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the electrochemical sensing performance of the modified electrode on chloramphenicol is respectively evaluated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry. According to the modified electrode, the sensitivity of an electrochemical signal is synergistically enhanced through the high-selectivity imprinting cavity of the molecularly imprinted polymer and the rapid and reversible oxidation-reduction activity of P2W18, the detection linear range of chloramphenicol is 0.1 nM to 10 [mu] M, and the detection limit of differential pulse voltammetry is as low as 0.145 nM; and a good application prospect is provided for preparing a uniform and controllable molecularly imprinted polymer composite modified electrode material in the future. At present, an indissolvable molecularly imprinted polymer material is integrated into an electrochemical sensor through an electrostatic layer-by-layer self-assembly technology, so that the research on improving the selectivity of chloramphenicol detection and reducing the detection limit is rarely reported.

The invention provides preparation of Bi (at) C / BiOCl heterojunction dispersion liquid, construction of a photoelectrochemical sensor and a method for detecting chloramphenicol. The preparation method comprises the following steps: mixing a BiOCl dispersion liquid and a Bi (at) C dispersion liquid, and performing ultrasonic dispersion to obtain a Bi (at) C / BiOCl heterojunction dispersion liquid; biOCl and Bi (at) C are compounded to prepare the Bi (at) C / BiOCl heterojunction photoelectric material, so that the spectral absorption range and the photon-generated carrier separation efficiency of a single material can be remarkably improved, and charge recombination is inhibited; the prepared Bi (at) C / BiOCl heterojunction material is used for electrode modification, and a photo-electrochemical sensor based on the Bi (at) C / BiOCl material, which is good in photoelectric property and stable in response signal, is obtained. The photoelectrochemical sensor provided by the invention realizes the detection of chloramphenicol, the adopted instrument is cheap and portable, the preparation method of the sensor is simple and feasible, the signal response is fast, the sensitivity is higher, and the detection limit is as low as 9.6 mu M.

The invention provides an Exiguobacterium sp. And an application thereof in degradation of chloramphenicol and microplastics. The strain is collected from a duck farm in Longping town, Guangdong province, is obtained through artificial enrichment culture, separation and purification, and is named as Exiguobacterium sp.CAP4. A high performance liquid chromatograph and a gas chromatograph are used for quantitative analysis, and the strain Exiguobacterium sp.CAP4 is found to have the capabilities of efficiently degrading chloramphenicol and mineralizing PE micro-plastics and different types of plastics, and can mineralize different types of plastics into CO2, so that the strain has the capabilities of efficiently degrading chloramphenicol and mineralizing PE micro-plastics and different types of plastics, and can be used for preparing CO2. The strain has the potential of being applied to environments such as livestock and poultry, municipal sewage and the like to realize biodegradation of chloramphenicol, micro-plastics and different types of plastics.

The application discloses a construction method and application of an electrochemiluminescence (ECL) antifouling sensor based on a zwitterionic hydrogel, and belongs to the fields of photoelectric sensing analysis, interface antifouling and environmental science and technology. In the application, a hydrogel based on a methacrylic acid sulfobetaine zwitterion and bovine serum albumin is used as an antifouling coating, so that the adsorption of biological proteins and bacteria is effectively hindered, and the service life of the sensor is prolonged. Meanwhile, an internal standard Au luminol is encapsulated in the antifouling hydrogel, so that the integration of interface antifouling and ratio detection is realized, the detection accuracy is improved, and the construction process of the ECL sensor is simplified. The constructed ECL antifouling sensor has high sensitivity for chloramphenicol detection, and the detection limit is 0.39 pM, so that the ECL antifouling sensor is suitable for trace detection of antibiotics in seawater.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

The present invention provides an endophytic fungus MD9F, a microbial agent and its application, belonging to the field of microbial technology. The endophytic fungus (Mycoleptodiscus sp.) strain MD9F provided by the present invention is isolated from the leaves of the medicinal plant Ophiopogon japonicus, identified as Mycoleptodiscus sp., and has been deposited in the General Microbiology Center of the China Center for Type Culture Collection on August 7, 2024, with the deposit number of CGMCC No. 3.28116. The endophytic fungus MD9F provided by the present invention is obtained through multiple activity screenings and isolation and purification, has significant chloramphenicol degradation ability, enriches the germplasm resource library of chloramphenicol-degrading bacteria, and provides an effective treatment approach for the environmental pollution of chloramphenicol antibiotics.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The invention relates to the field of microbiology, in particular to a cassava root rot pathogen sampling preservation reagent and a preparation method and application thereof, and the cassava root rot pathogen sampling preservation reagent comprises the following raw materials: modified lignin nanoparticles, sorbitol, a trehalose-proline compound, a CTAB buffer solution, ascorbic acid, chloramphenicol, glucose, dimethyl sulfoxide and glycerol. The modified lignin nano-particles, the sorbitol and the trehalose-proline compound play a role through a synergistic effect, the modified lignin nano-particles absorb free heavy metals in the preservation reagent, then the sorbitol competes with metal ion binding sites, the probability that the sorbitol makes contact with catalytic sites such as glycerol is reduced, and the preservation reagent has a good preservation effect on heavy metals. Finally, the trehalose-proline compound cooperates with the trehalose and the proline to prevent re-dissolution of metal ions and degradation of reagent components. The three components act together to form a dynamic balance system, so that the application range, the preparation method and the application effect of the efficient preservation reagent are obtained.

The present invention discloses a photoelectrochemical sensor for detecting chloramphenicol and a preparation method thereof, belonging to the technical field of electrochemical sensors. The sensor comprises: a titanium substrate, titanium dioxide nanotubes formed by oxidation on the titanium substrate, a molecularly imprinted membrane modified on the surface of the titanium dioxide nanotubes, a counter electrode, and a reference electrode; the molecularly imprinted membrane is a chloramphenicol molecularly imprinted membrane formed by polymerizing chloramphenicol and aniline on a working electrode through an electrode reaction, after which the chloramphenicol is removed; the titanium dioxide nanotubes constitute the working electrode, and the molecularly imprinted membrane constitutes the working surface of the working electrode. The sensor device of the present invention has the characteristics of high sensitivity, rapid detection, and good stability in the detection of chloramphenicol. The principle of oxidizing the imprinted membrane by generating photogenerated holes under illumination is utilized, so that the device can self-clean and achieve the purpose of being used multiple times.

The present invention relates to a preparation method of a chloramphenicol double molecular imprinted membrane electrochemical sensor. The present invention uses chloramphenicol as a template molecule and N-(4-pentenoyl) isoleucyl-chitosan oligosaccharide as a functional monomer oligomer. Under the action of a cross-linking agent and an initiator, a chloramphenicol molecular imprinted membrane is first formed by thermal polymerization on the surface of a glassy carbon electrode. Then, electrochemical polymerization is carried out in a pyrrole solution containing chloramphenicol to form a composite double molecular imprinted membrane. When the sensor prepared by the present invention is used for chloramphenicol concentration determination, specificity, sensitivity and stability are all excellent. The electroactive probe during detection can be potassium ferrocyanide, ferrocenylmethanol, etc.; the prepared sensor is a double molecular imprinted performance, contrasting the molecular imprinted membrane of a monolayer, significantly improving the recognition performance of the composite molecular imprinted membrane to chloramphenicol, and the detection limit reaches nM level.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a relieving and acne removing spray and application, the spray comprises an agent A and an agent B, wherein the agent A is prepared from the following raw materials in percentage by mass: 2 to 3 percent of humectants, 1 to 1.5 percent of penetrating agents, 0.1 to 0.2 percent of salicylic acid microcapsules, 0.1 to 0.2 percent of preservatives, 0.6 to 0.8 percent of conditioning agents, 0.05 to 0.15 percent of buffering agents, 1.5 to 1.8 percent of plant extracts and the balance of deionized water; the agent B is prepared from the following raw materials in percentage by mass: 10 to 12 percent of chloramphenicol and the balance of solvent. The soothing acne-removing spray prepared by the invention has the advantages of quick effect and no irritation.

The invention relates to the technical field of environmental treatment, in particular to a method for degrading chloramphenicol by using a co-metabolism system of chlamydomonas reinhardtii and isoleucine. Isoleucine and chlamydomonas reinhardtii form a co-metabolism system, the degradation efficiency and degradation rate of chlamydomonas reinhardtii on chloramphenicol are greatly improved, high-efficiency degradation of chloramphenicol can be achieved, meanwhile, proliferation and propagation of chloramphenicol drug-resistant genes can be blocked, and the method is a chloramphenicol pollution efficient remediation strategy which is low in price and environmentally friendly.

The application discloses a kind of magnetic nano-enzyme microspheres and preparation method and application thereof, belong to the cross technical field of material, drug, rapid detection etc..The magnetic nano-enzyme microspheres take Fe3O4 microspheres as core, and platinum nanoparticles are in situ grown after being coated with dendritic mesoporous silica on the surface.The magnetic nano-enzyme microspheres of the application utilize magnetic response separation, high load content and enzyme catalytic amplification, greatly improve the detection sensitivity and signal strength, and are suitable for high-sensitivity detection of trace chloramphenicol and hexachlorobenzene.The qualitative detection cutoff value of chloramphenicol is 0.1 ng / mL, and the quantitative detection sensitivity is 0.0011 ng / mL.The quantitative detection sensitivity of hexachlorobenzene is 0.4 ng / mL.

The invention discloses a phosphorus-solubilizing fungus obtaining and optimizing method, and belongs to the field of environment and the field of agricultural biotechnology. The method comprises the following steps: collecting a soil sample of Inner Mongolia Tin-Go Union; performing liquid culture on the soil sample, and performing gradient dilution under a sterile condition; the method comprises the following steps: sucking 100 [mu] L of bacterial solutions with different concentrations, coating the bacterial solutions on a PVK solid culture medium added with chloramphenicol and streptomycin, culturing, selecting single colonies with good growth, typical characteristics and large and remarkable phosphate solubilizing circle, continuously carrying out streak purification on the PVK solid culture medium to obtain a target strain pure culture, preserving and identifying to obtain the phosphate solubilizing fungi; optimizing culture conditions: taking glucose as a unique carbon source, wherein the concentration of the glucose is 15g / L; ammonium chloride is used as a sole nitrogen source, and the concentration of the ammonium chloride is 0.082 g / L; the culture temperature is 30 DEG C; the initial pH is 5; the phosphorus solubilizing amount of the phosphorus solubilizing fungi is 858.31 mg / L.

The invention discloses an IS26-based drug-resistant gene resistance and control plasmid, an engineering bacterium, a fungicide and application, and belongs to the technical field of gene engineering, and the nucleotide sequence of the IS26-based drug-resistant gene resistance and control plasmid is shown as SEQ ID NO: 1 or SEQ ID NO: 2. Engineering bacteria containing the drug-resistant gene resistance and control plasmid and a microbial inoculum can be used for resistance and control of drug-resistant genes, and the insertion sequence IS26 is specifically destroyed, so that the number of the drug-resistant genes is fundamentally reduced, spreading of the drug-resistant genes is blocked, and a precise solution is provided for treatment of drug resistance; the compound has broad-spectrum drug-resistant bacterium resistance and control performance, gentamicin, tetracycline, chloramphenicol and ampicillin drug-resistant bacteria in wastewater can be effectively prevented and controlled, and the highest resistance and control efficiency can reach 83.76%; the plasmid, the engineering bacterium and the fungicide are low in preparation cost, can be used for in-situ wastewater treatment, are low in treatment cost and good in effect, and have wide application prospects.

The invention discloses a porous carbon electrode based on gold nanoparticles / poly (3, 4)-ethylenedioxythiophene, a preparation method of the porous carbon electrode and application of the porous carbon electrode in antibiotic detection, and belongs to the technical field of food. The preparation method based on the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode comprises the following steps: (1) engraving the porous carbon electrode by using a laser engraving machine; (2) putting the carbon electrode into a (3, 4)-ethylenedioxythiophene solution, and polymerizing (3, 4)-ethylenedioxythiophene on the porous carbon electrode by utilizing a potentiostatic method; (3) placing the electrode prepared in the step (2) in a chloroauric acid solution, and depositing gold nanoparticles on the electrode through cyclic voltammetry; the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode provided by the invention can sensitively detect chloramphenicol and enrofloxacin at the same time, and can be used for detecting chloramphenicol and enrofloxacin in tap water.

The invention belongs to the technical field of aureomycin detection, and particularly relates to a preparation method of a carbon quantum dot ratiometric fluorescent probe and a method for detecting aureomycin. The method is based on the characteristic that aureomycin can enable a carbon quantum dot ratio fluorescent probe to correspondingly detect that the color of a system gradually changes from yellow to yellow green to dark blue to light purple to final dark purple under an ultraviolet lamp, and obvious changes occur. Detecting the fluorescence intensity change R425 / R515 of a mixed solution of a to-be-detected sample and D-CDs, and comparing with the standard curve to obtain the aureomycin content in the to-be-detected sample. Besides, a paper-based sensor containing the carbon quantum dot ratio fluorescent probe is prepared, a smart phone is used as a signal reader, carrying is convenient, on-site detection of aureomycin in a limited-resource environment can be achieved, and the detection method is simple, sensitive and efficient.