58 results about "Chloramphenicol" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The invention discloses a pseudomonas capable of efficiently and rapidly degrading chloramphenicol and application of the pseudomonas. The name of the strain is Pseudomonas sp. MH-W1, the preservation number of the strain is GDMCC No: 66907, and the strain is preserved in the Guangdong Microbial Culture Collection Center on September 1, 2025. The strain can tolerate 200 mg / L of chloramphenicol; 100 mg / L of chloramphenicol can be degraded within 8 h, and the degradation rate reaches about 80%. According to the method provided by the invention, the degradation time can be remarkably shortened, the degradation efficiency is improved, the degradation condition is mild, the operation is simple and convenient, large-scale application is easy to realize, and the method has a wide industrial popularization prospect. The strain is an aerobic bacterium, is fast in growth and reproduction, simple to prepare, low in cost, convenient to use and high in efficiency, and can be used for repairing the environment polluted by chloramphenicol.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The invention discloses a method for strengthening three-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in rice field soil through sulfurized zero-valent iron, and belongs to the technical field of rice field soil pollution remediation. The sulfurized zero-valent iron (S-ZVI) is prepared according to the sulfur-iron ratio of 0.1-0.2; the S-ZVI is mixed with the contaminated soil, and rice is cultivated in cooperation with a specific fertilization and irrigation scheme. According to the method for strengthening the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in the rice field soil through the sulfurized zero-valent iron, by optimizing preparation and application of the sulfurized zero-valent iron, under the synergistic effect of chemical reduction and microbial degradation, the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is expected to be achieved, and the effect of strengthening the rice field soil chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is achieved. And an important means is provided for degradation of antibiotics in rice field soil.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The present invention relates to medicine and the pharmaceutical industry, and more particularly to agents capable of inhibiting inflammatory processes, and even more particularly to agents that have an antimicrobial, sorptive, angioprotective and wound-healing effect on local inflammatory processes of the skin and soft tissues, for use in surgery, traumatology and dermatology. What is proposed in the present invention is a combination including dexpanthenol, methyluracil and chloramphenicol, and also a pharmaceutical composition based thereon.

The invention discloses a chloramphenicol hapten, antigen, antibody and colloidal gold detection device as well as a preparation method and application thereof. According to the chloramphenicol hapten prepared by using a total synthesis method, all potential recognition sites of chloramphenicol are reserved to the maximum extent, particularly, the most critical dichloro structure of chloramphenicol is reserved for the first time, after a spacer arm is introduced, the distribution of electron cloud of the hapten is almost consistent with that of overall electron cloud of chloramphenicol, and the immunogenicity of the chloramphenicol antigen is improved; the chloramphenicol antigen and the monoclonal antibody prepared by the invention are high in specificity when being used for ELISA detection, the IC50 value is 8.21 pg / mL, the linear detection range is 1.71-39.45 pg / mL, and the cross reaction rates of the chloramphenicol antigen and the monoclonal antibody to common structural analogues are lower than 0.01%; and the sensitivity to chloramphenicol in a sample is 0.1 g / kg.

The invention relates to a method for electrochemically detecting chloramphenicol by using molecular imprinting. The method is characterized in that a chloramphenicol molecularly imprinted polymer prepared from a temperature-sensitive monomer N-isopropylacrylamide and Dawson type tungsten phosphate (P2W18) are jointly modified on a substrate electrode through a simple and controllable electrostatic layer-by-layer self-assembly method. The modified electrode synthesized by the method disclosed by the invention is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and the electrochemical sensing performance of the modified electrode on chloramphenicol is respectively evaluated by cyclic voltammetry, differential pulse voltammetry and square wave voltammetry. According to the modified electrode, the sensitivity of an electrochemical signal is synergistically enhanced through the high-selectivity imprinting cavity of the molecularly imprinted polymer and the rapid and reversible oxidation-reduction activity of P2W18, the detection linear range of chloramphenicol is 0.1 nM to 10 [mu] M, and the detection limit of differential pulse voltammetry is as low as 0.145 nM; and a good application prospect is provided for preparing a uniform and controllable molecularly imprinted polymer composite modified electrode material in the future. At present, an indissolvable molecularly imprinted polymer material is integrated into an electrochemical sensor through an electrostatic layer-by-layer self-assembly technology, so that the research on improving the selectivity of chloramphenicol detection and reducing the detection limit is rarely reported.

The invention provides preparation of Bi (at) C / BiOCl heterojunction dispersion liquid, construction of a photoelectrochemical sensor and a method for detecting chloramphenicol. The preparation method comprises the following steps: mixing a BiOCl dispersion liquid and a Bi (at) C dispersion liquid, and performing ultrasonic dispersion to obtain a Bi (at) C / BiOCl heterojunction dispersion liquid; biOCl and Bi (at) C are compounded to prepare the Bi (at) C / BiOCl heterojunction photoelectric material, so that the spectral absorption range and the photon-generated carrier separation efficiency of a single material can be remarkably improved, and charge recombination is inhibited; the prepared Bi (at) C / BiOCl heterojunction material is used for electrode modification, and a photo-electrochemical sensor based on the Bi (at) C / BiOCl material, which is good in photoelectric property and stable in response signal, is obtained. The photoelectrochemical sensor provided by the invention realizes the detection of chloramphenicol, the adopted instrument is cheap and portable, the preparation method of the sensor is simple and feasible, the signal response is fast, the sensitivity is higher, and the detection limit is as low as 9.6 mu M.

The application discloses a construction method and application of an electrochemiluminescence (ECL) antifouling sensor based on a zwitterionic hydrogel, and belongs to the fields of photoelectric sensing analysis, interface antifouling and environmental science and technology. In the application, a hydrogel based on a methacrylic acid sulfobetaine zwitterion and bovine serum albumin is used as an antifouling coating, so that the adsorption of biological proteins and bacteria is effectively hindered, and the service life of the sensor is prolonged. Meanwhile, an internal standard Au luminol is encapsulated in the antifouling hydrogel, so that the integration of interface antifouling and ratio detection is realized, the detection accuracy is improved, and the construction process of the ECL sensor is simplified. The constructed ECL antifouling sensor has high sensitivity for chloramphenicol detection, and the detection limit is 0.39 pM, so that the ECL antifouling sensor is suitable for trace detection of antibiotics in seawater.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The invention relates to the technical field of environmental treatment, in particular to a method for degrading chloramphenicol by using a co-metabolism system of chlamydomonas reinhardtii and isoleucine. Isoleucine and chlamydomonas reinhardtii form a co-metabolism system, the degradation efficiency and degradation rate of chlamydomonas reinhardtii on chloramphenicol are greatly improved, high-efficiency degradation of chloramphenicol can be achieved, meanwhile, proliferation and propagation of chloramphenicol drug-resistant genes can be blocked, and the method is a chloramphenicol pollution efficient remediation strategy which is low in price and environmentally friendly.

The application discloses a kind of magnetic nano-enzyme microspheres and preparation method and application thereof, belong to the cross technical field of material, drug, rapid detection etc..The magnetic nano-enzyme microspheres take Fe3O4 microspheres as core, and platinum nanoparticles are in situ grown after being coated with dendritic mesoporous silica on the surface.The magnetic nano-enzyme microspheres of the application utilize magnetic response separation, high load content and enzyme catalytic amplification, greatly improve the detection sensitivity and signal strength, and are suitable for high-sensitivity detection of trace chloramphenicol and hexachlorobenzene.The qualitative detection cutoff value of chloramphenicol is 0.1 ng / mL, and the quantitative detection sensitivity is 0.0011 ng / mL.The quantitative detection sensitivity of hexachlorobenzene is 0.4 ng / mL.

The invention discloses a phosphorus-solubilizing fungus obtaining and optimizing method, and belongs to the field of environment and the field of agricultural biotechnology. The method comprises the following steps: collecting a soil sample of Inner Mongolia Tin-Go Union; performing liquid culture on the soil sample, and performing gradient dilution under a sterile condition; the method comprises the following steps: sucking 100 [mu] L of bacterial solutions with different concentrations, coating the bacterial solutions on a PVK solid culture medium added with chloramphenicol and streptomycin, culturing, selecting single colonies with good growth, typical characteristics and large and remarkable phosphate solubilizing circle, continuously carrying out streak purification on the PVK solid culture medium to obtain a target strain pure culture, preserving and identifying to obtain the phosphate solubilizing fungi; optimizing culture conditions: taking glucose as a unique carbon source, wherein the concentration of the glucose is 15g / L; ammonium chloride is used as a sole nitrogen source, and the concentration of the ammonium chloride is 0.082 g / L; the culture temperature is 30 DEG C; the initial pH is 5; the phosphorus solubilizing amount of the phosphorus solubilizing fungi is 858.31 mg / L.

The invention discloses a porous carbon electrode based on gold nanoparticles / poly (3, 4)-ethylenedioxythiophene, a preparation method of the porous carbon electrode and application of the porous carbon electrode in antibiotic detection, and belongs to the technical field of food. The preparation method based on the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode comprises the following steps: (1) engraving the porous carbon electrode by using a laser engraving machine; (2) putting the carbon electrode into a (3, 4)-ethylenedioxythiophene solution, and polymerizing (3, 4)-ethylenedioxythiophene on the porous carbon electrode by utilizing a potentiostatic method; (3) placing the electrode prepared in the step (2) in a chloroauric acid solution, and depositing gold nanoparticles on the electrode through cyclic voltammetry; the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode provided by the invention can sensitively detect chloramphenicol and enrofloxacin at the same time, and can be used for detecting chloramphenicol and enrofloxacin in tap water.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

This invention discloses a method for preparing a zirconium-based metal-organic framework@nickel-cobalt metal-organic framework oxygen reduction catalyst and its application in the removal of chloramphenicol. The method uses a nickel-cobalt metal-organic framework as a substrate, and fuses a zirconium-based metal-organic framework onto it via a hydrothermal method to form an octahedral zirconium-based metal-organic framework@nickel-cobalt metal-organic framework. This structure significantly improves the accessibility of adsorption sites and electrocatalytic active sites while maintaining the structural integrity of the metal-organic framework. The oxygen reduction performance was tested, and the system was coupled with an electroactive biofilm to determine the degradation efficiency of chloramphenicol within 244 hours. The results show that the zirconium-based metal-organic framework@nickel-cobalt metal-organic framework exhibits excellent oxygen reduction performance. In the modified microbial fuel cell, the degradation efficiency of chloramphenicol reached 90.31% within 244 hours, and the concentration decreased from 18.62 mg / L to 1.80 mg / L. This study provides a new approach for constructing efficient and stable metal-organic framework-based electrocatalysts, and has good application prospects in the fields of antibiotic wastewater treatment and environmental remediation.

This invention discloses a rapid and selective detection and monitoring culture medium for Candida auris, its preparation method, and its application, belonging to the field of microbial detection technology. The culture medium comprises the following components: peptone, yeast extract, glucose, agar, selectivity inhibitor, chromogenic substrate, and auxiliary components. The chromogenic substrate consists of euonymus alcohol and X-Gal. The culture medium of this invention inhibits contaminating bacteria by adding chloramphenicol and ciclopirox ol, and the chromogenic system of euonymus alcohol and X-Gal achieves specific detection of Candida auris. This culture medium has the advantages of high selectivity, rapid identification, and low cost, and is suitable for clinical samples and environmental monitoring.

The invention relates to an inonotus obliquus fermentation product and application of the inonotus obliquus fermentation product in hypoglycemic drugs, and belongs to the technical field of inonotus obliquus fermentation products. A component culture medium is prepared from 10-15 parts of plant flour, 3-6 parts of glucose, 0.8-1.2 parts of dipotassium phosphate, 1-2 parts of magnesium sulfate, 5-8 parts of protein peptide, 4-8 parts of agar and 60-80 parts of purified water; the component B culture medium is prepared from the following raw materials in parts by weight: 12-15 parts of plant starch, 2-4 parts of glucose, 1-3 parts of oxytetracycline, 1-3 parts of chloramphenicol, 8-16 parts of sodium hyaluronate and 60-80 parts of purified water. Through the multi-target synergistic effect of improving insulin resistance, protecting islet cells, inhibiting sugar absorption and the like, the hypoglycemic effect is obvious, and the prepared inonotus obliquus fermentation product is simple in process, low in operation difficulty, suitable for industrial production and capable of being applied to the fields of cosmetics, textile industry and medicine.

The present application relates to the construction and application of the EcORep-based continuous directed evolution reporter system of aminoacyl-tRNA synthetase, and specifically, the reporter system comprises: a green fluorescent protein mutant sfGFP containing amber stop codon TAG N150TAG ; a chloramphenicol acetyltransferase mutant CAT containing amber stop codon TAG D112TAG ; a linear replicon containing aminoacyl-tRNA synthetase; a reporter plasmid containing a plus-strand mismatch DNA polymerase to continuously mutate the linear replicon, thereby generating an in-vivo library of aminoacyl-tRNA synthetase mutants; and a tRNA, which is orthogonal to the aminoacyl-tRNA synthetase and enables the incorporation of unnatural amino acids and the complete expression of proteins. The present application proves that the evolution reporter system can realize the efficient directed evolution of aminoacyl-tRNA synthetase, and further expands the application range of genetic code expansion technology.

The invention relates to a capsule for treating gynecological diseases and a preparation method thereof. The capsule is prepared from polygonum cuspidatum, sargentgloryvine stem, coptis chinensis, rhizoma bletillae, radix gentianae, dragon's blood, cuttlebone, rheum officinale, aloe, catechu, astragalus membranaceus, chloramphenicol and purified water. The capsule is prepared through a powder preparation process, a conventional vaginal medicine filling method is adopted for administration, the capsule is dissolved by virtue of vaginal secretion to release medicine, and the medicine acts on the vagina, the cervix uteri and the endometrium and directly reaches a focus, so that the effects of diminishing inflammation, sterilizing, resisting viruses, diminishing swelling, removing stasis and repairing mucosa are achieved; the traditional Chinese medicine composition can be used for treating colpitis mycotica, infectious microbe vaginitis, cervical erosion, cervicitis, HPV infection and endometritis. The capsule is a proved recipe inherited by several generations, has a good curative effect, takes traditional Chinese medicines as main raw materials, is added with a small amount of western medicines, has the advantages of benefiting and eliminating disadvantages, has no side effect, no preservative and no irritation, has an effective rate of 99% and a cure rate of 98%, and is worthy of popularization.

The application discloses a pseudomonas for degrading chloramphenicol and application thereof. Pseudomonas The strain is named as Pseudomonas sp. MH-W1, the preservation number is GDMCC No: 66907, and the strain is preserved in the Guangdong Microbial Culture Collection Center on September 1, 2025. The strain can tolerate 200 mg / L chloramphenicol; and can degrade 100 mg / L chloramphenicol in 8 hours, and the degradation rate is about 80%. The method provided by the application can significantly shorten the degradation time and improve the degradation efficiency, the degradation condition is mild, the operation is simple, large-scale application is easy to realize, and the method has a wide industrialization popularization prospect. The strain is an aerobic bacterium, has fast growth and reproduction, is simple to prepare, low in cost, convenient to use and high in efficiency, and can be used for repairing chloramphenicol contaminated environment.

This invention relates to a method for isolating Pichia pastoris. The method includes sample preparation: grinding a medium- and high-temperature fermented koji sample and then serially diluting it to obtain a bacterial suspension; selective plating: plating the bacterial suspension onto a selective culture medium; and high-temperature isothermal incubation: culturing the plated selective culture medium at 38℃-42℃ until single colonies grow; wherein the selective culture medium is YPD basal medium supplemented with chloramphenicol and acetic acid. This method effectively inactivates background bacteria, selectively enriches the target strain, and significantly increases the ethyl acetate yield of the obtained strain.

The application relates to the field of ophthalmic drugs, and particularly discloses an eye drop, which comprises the following components in mass fraction: 0.003%-0.2% of chlorhexidine gluconate; and 0.025%-0.05% of chloramphenicol. Since acanthamoeba usually feeds on bacteria, the chlorhexidine gluconate is used as a main component to kill acanthamoeba in the application, and the antibiotic chloramphenicol is added, so that the keratitis caused by bacterial infection can be avoided, the ocular surface bacteria can be killed, the nutrition source of acanthamoeba can be reduced, and the treatment of acanthamoeba keratitis can be synergistically strengthened.

The invention discloses a solid-phase extraction process method for detecting veterinary drug residues in an animal-derived sample, and belongs to the technical field of analysis of harmful residues in food. The method comprises the steps of sample pretreatment, solid-phase extraction column activation balance, sample loading, multi-stage washing and core collaborative elution. Especially, core collaborative elution adopts a collaborative elution method combining three-stage pH gradient elution and two-time competitive acetone pulse injection. The method comprises the following steps: firstly, eluting sulfonamides and quinolones under an acidic condition; then injecting acetone to competitively remove phospholipid; then, the tetracycline and macrolide drugs are eluted under the neutral condition; injecting acetone again for deep purification; and finally, eluting the chloramphenicol medicine under an alkaline condition. According to the method, the problem that the recovery rate and the purification degree are difficult to consider when multiple types of veterinary drug residues in the egg high-phospholipid complex matrix are synchronously detected is effectively solved, and the accuracy, the sensitivity and the reproducibility of subsequent LC-MS / MS analysis are remarkably improved.

The application relates to a new pesticide-degrading strain, in particular to a strain New bacteria Acinetobacter oryzae F9-1 . A strain F9-1 is obtained by separation, screening and identification from a soil sample in Hami City of Xinjiang Uygur Autonomous Region New bacteria Acinetobacter oryzae F9- 1 The strain is subjected to physiological and biochemical characteristic detection, the strain F9-1 shows small colonies, smooth surface, white semi-transparency on NB plates, and the results of gram staining, contact enzyme and indole experiment are positive, and the results of oxidase, V.P test and urease are negative. The strain F9-1 can hydrolyze hydrogen peroxide, can utilize L-alanine, lactic acid, L-aspartic acid and quinine acid, is sensitive to 8% NaCl, lincomycin, naphthylidine ketone acid and chloromycetin, and can degrade fluazifop-butyl and pendimethalin, and has the advantages of environmental protection, high efficiency, economy and sustainability, and is an effective means for solving the problem of pesticide pollution.

The invention relates to a method for separating and purifying ascospores of cordyceps sinensis strains, and belongs to the field of ascospore strain separation and culture. The method comprises the following steps: firstly, removing impurities such as bacteria and fungi through gradient forward flushing and efficiently recovering target ascospores through reverse flushing by virtue of a three-stage gradient volume flushing, filtering and purifying process; performing primary culture and isolated culture on the filtered and purified ascospores, adding chloramphenicol into a culture medium to inhibit bacterial pollution, and simulating an ascospore primary environment to perform constant-temperature and constant-humidity culture; and after the bacterial colony is visible to the naked eyes, transferring and germinating the monospores into the culture medium by adopting a metal agar gel puncher, and continuously culturing to obtain the pure cordyceps sinensis mycelia developed from the single ascospores. The invention provides a separation and purification method for efficiently, purely and controllably separating the cordyceps sinensis fungus ascospores, effectively breaking the dormancy of the cordyceps sinensis fungus ascospores and remarkably improving the germination rate, and a high-purity and active cordyceps sinensis fungus pure culture can be obtained.