Process for producing chloromycetin molecular engram polymer microsphere

A molecularly imprinted polymer technology, applied in the field of bioengineering, can solve the problems of molecularly imprinted polymer molecular recognition and selectivity decline, irregular particle shape, low yield, etc., to achieve great promotion and application value, accurate separation, The effect of high-efficiency enrichment

Inactive Publication Date: 2006-08-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that molecularly imprinted polymer particles with the desired particle size need to be obtained through cumbersome procedures such as sieving, and it has disadvantages such as low yield, irregular particle shape, and poor dispersion.
The grinding process also leads to a decrease in the molecular recognition and selectivity of molecularly imprinted polymers

Method used

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  • Process for producing chloromycetin molecular engram polymer microsphere
  • Process for producing chloromycetin molecular engram polymer microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Add 100ml of 4% PVA1788 into the four-neck flask, blow nitrogen gas for 10min, and stir at the same time, 400rpm.

[0024] In another container, 0.8ml DAM, 5ml EDMA, 10ml octanol and 0.32g chloramphenicol from which the polymerization inhibitor has been removed were mixed ultrasonically for 20min, then 120mg AIBN and 5ml chloroform were added to continue ultrasonication for 5min, and ice was added during ultrasonication. block, keep cold.

[0025] Keep the stirring speed, and add the prepared monomer phase into the four-neck flask to ensure good monomer dispersion. Continuous nitrogen flow, 70°C, 24h. The resulting polymer was subjected to elution treatment.

[0026] After the polymerization reaction finishes, the obtained polymer is filtered out and rinsed with water three times, then poured into a 200ml beaker, stirred with 80°C double distilled water for 60min, washed three times with 50ml methanol, twice with 50ml acetone (washing away swelling agent, residual org...

Embodiment 2

[0028] Add 100ml of 4% PVA1788 into the four-neck flask, blow nitrogen gas for 10min, and stir at the same time, 400rpm.

[0029] In another container, 0.8ml St, 5ml EGDMA and 10ml octanol, 0.32g chloramphenicol that have removed polymerization inhibitor, other operation is the same as embodiment 1. Finally, microspheres with an average particle size of about 120 μm and many small holes on the surface are obtained, and the inventive effect is basically equivalent to that of figure 1 , 2 shown.

Embodiment 3

[0031] Add 100ml of 4% PVA1788 into the four-necked flask, blow nitrogen gas for 10min, and stir at the same time, 350rpm.

[0032] In another container, 0.8ml DAM, 5ml EGDMA, 10ml octanol and 0.32g chloramphenicol from which the polymerization inhibitor has been removed were ultrasonically mixed for 20min, then 120mg AIBN and 5ml chloroform were added to continue ultrasonication for 5min, and ice was added during ultrasonication. block, keep cold.

[0033] Keep the stirring speed, and add the prepared monomer phase into the four-neck flask to ensure good monomer dispersion. Continuous nitrogen flow, 70°C, 24h. Other operations are the same as in Example 1. Finally, microspheres with an average particle size of about 90 μm and many small holes on the surface are obtained, and the inventive effect is basically equivalent to that of figure 1 , 2 shown.

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Abstract

The present invention relates to a preparation method of chloromycetin molecular engram polymer microsphere, belonging to field of biological engineering technology. Its preparation method includes the following concrete steps: the mole ratio of template molecule, functional monomer and ethylene glycol dimethacrylate is 1:2-8:25; volume ratio of total volume of diluting agent and ethylene glycol dimethacrylate and water is 1:6-14; dissolving polyvinyl alcohol 1788 in water, dissolving functional monomer and template molecule in diluting agent; mixing ethylene glycol dimethacrylate, azobisisobutyronitrile and diluting agent; mixing the above-mentioned three solutions, after the reaction is completed, stirring product in water, cooling and filtering, washing successively by using double-distilled water, methyl alcohol and acetone, utilizing Soxhletí»s extraction process to remove template molecule; vacuum drying so as to obtain the invented chloromycetin molecular engram polymer microsphere.

Description

technical field [0001] The invention relates to a method in the technical field of bioengineering, in particular to a method for preparing chloramphenicol molecularly imprinted polymer microspheres. Background technique [0002] Chloramphenicol (CAP) is a broad-spectrum antibiotic first isolated from microbial metabolites in 1947. However, because of the nitro group on its benzene ring, its decomposition half-life is long and it has serious side effects. Therefore, chloramphenicol residues in animal foods and aquatic products pose a huge threat to human health. At present, the detection methods of chloramphenicol residue mainly include microbiological method, immunological method, chromatographic method, etc., and some combined techniques are used. Microbial method has disadvantages such as poor specificity, low sensitivity, high detection limit (> 3mg / kg); immunoassay is an analytical technique based on the specific binding reaction between antigen and antibody, but whe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/60B01J20/281
Inventor 张大兵武爱波史西志李荣秀
Owner SHANGHAI JIAO TONG UNIV
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