154 results about "Molecularly imprinted polymer" patented technology

The invention relates to the field of material chemistry on-line detection, in particular to a 1, 2, 4-trichlorobenzene on-line detection material and a preparation method thereof, and provides a film-forming sensitive layer material capable of being coated on the surface of a transducer, and the film-forming sensitive layer material comprises chitosan, a crosslinking component with the content of 0.1-5wt% in terms of glutaraldehyde and magnetic core-shell molecularly imprinted particles after being dried, the magnetic core-shell molecularly imprinted particle contains a ferroferric oxide magnetic inner core, a silicon dioxide middle layer and a molecularly imprinted polymer shell layer, and the shell layer is a cyclodextrin methacrylate and ethylene glycol dimethacrylate copolymer network and takes 1, 2, 4-trichlorobenzene as a template. The material keeps the membrane integrity and long-term stability under the working conditions of circulation and regeneration and also gives consideration to quick response, and the residual 1, 2, 4-trichlorobenzene is controlled to be not greater than 0.10 wt% and the residual glutaraldehyde is controlled to be not greater than 0.5 wt%. The method is used for relieving structural mass transfer contradiction and processing and coating coupling contradiction caused by high filler load, and has online detection application value.

The invention relates to the technical field of health wine preparation, and discloses a multi-effect synergistic enhanced bear gall body-building wine compound preparation process which comprises the following steps: taking sorghum, corn and fructus lycii as fermentation base materials, and carrying out double-strain synergistic fermentation by adopting saccharomyces cerevisiae and lactobacillus plantarum; adding fresh ursodesoxycholic acid during fermentation, carrying out in-situ enzymatic debinding conversion by using a compound enzyme system generated by a fermentation system, and converting the bound bile acid into free ursodesoxycholic acid; after fermentation, distilling to obtain raw wine; a molecularly imprinted polymer prepared by taking a wolfberry polyphenol key monomer as a template molecule is adopted to carry out targeted anchoring refining on raw wine, selective capture and enrichment of wolfberry polyphenol are carried out, and finally aging is carried out to obtain a finished product. According to the method disclosed by the invention, upstream double-strain fermentation in-situ enzymatic conversion and downstream molecular imprinting targeted refining are systematically integrated, so that the technical contradictions that the conversion rate of combined bile acid is low and a large amount of polyphenol functional components are synchronously lost during purification and impurity removal in a traditional process are solved.

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

The present application belongs to the technical field of adsorbing materials, and particularly relates to a titanium dioxide molecular imprinting polymer as well as a preparation method and application thereof. The present application uses Sudan red as a template molecule, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent, and azobisisobutyronitrile as an initiator to construct a cavity structure matching the Sudan red molecule on the surface of the amino-modified titanium dioxide ultrafine particles, and successfully prepares the titanium dioxide molecular imprinting polymer. The material combines the photocatalytic characteristics of the titanium dioxide and the selective recognition ability of the molecular imprinting, can degrade the Sudan red under light, and has a higher photocatalytic efficiency than that of the pure titanium dioxide. The material synthesized through the surface imprinting technology has a three-dimensional cavity complementary to the template molecule, and has a larger adsorption capacity than that of the non-imprinted material. The material realizes the integration of the selective recognition, adsorption and degradation of the Sudan red, and is suitable for complex industrial environments.

The invention discloses an interface molecularly imprinted polymer microsphere as well as a preparation method and application thereof, and relates to the technical field of functional polymer materials. The preparation method comprises the following steps: firstly, enabling template molecules to react with a silane coupling agent to form a template molecule compound; and then anchoring the template molecule compound on the surface of SiO2 particles, so that the template molecule compound simultaneously plays the dual roles of a stabilizer and an imprinting template in Pickering emulsion polymerization. And after the polymerization is completed, removing the template molecule modified SiO2 particles to obtain the polymer microspheres of which the imprinting sites are completely exposed on the surfaces of the microspheres. Molecular imprinting sites of the microspheres are uniformly distributed on the surface and are in an open and highly accessible state, so that the recognition rate and the binding capacity of target molecules are remarkably improved, and the microspheres have excellent selective enrichment and separation performance on target analytes; a reliable technical path is provided for practical application of the molecularly imprinted material in the fields of solid-phase extraction, sensing detection, medicine purification and the like.

The invention discloses a preparation method of curcumin, and belongs to the technical field of natural product extraction. The method comprises the following steps: firstly, adopting a modified deep-eutectic solvent into which propyl gallate is introduced as an extraction medium, and realizing high-selectivity extraction of curcumin through a special molecular structure of the modified deep-eutectic solvent; then the ultrasonic-microwave coupling technology is combined, so that the extraction efficiency is remarkably improved; finally, the modified molecularly imprinted polymer with magnetism is used for precise purification, the material has the specific recognition capacity and the rapid magnetic separation characteristic, polyethylene glycol is introduced to serve as a pore-foaming agent, and more active sites are provided for adsorption of curcumin molecules. According to the method disclosed by the invention, through collaborative innovation of all links, the whole process optimization from extraction to purification is realized, the product purity and the extraction efficiency are remarkably improved, the energy consumption and the solvent pollution are greatly reduced, and a reliable solution is provided for industrial production of the high-quality curcumin.

The invention provides a preparation method and application of a doxycycline (DOX) molecularly imprinted surface plasmon resonance (SPR) sensing chip. According to the chip, an SPR bare gold chip is used as a substrate, DOX is used as a template molecule, a molecularly imprinted polymer (MIP) recognition membrane is constructed on the surface of the chip, and collaborative integration of the MIP technology and the SPR technology is achieved. According to the strategy, the chip is endowed with specific recognition capability on template molecules DOX, and local refractive index change caused by combination of the DOX and the recognition film can be detected in real time in an unmarked manner, so that the detection sensitivity and accuracy are remarkably improved. By utilizing an SPR (Surface Plasmon Resonance) analyzer, the chip can realize rapid quantitative detection of DOX in a low concentration range, shows good reproducibility, and can be applied to detection of actual samples.

The invention belongs to the technical field of chrysin detection, and particularly relates to a dual-emission fluorescent molecularly imprinted polymer as well as a preparation method and application thereof. The method comprises the following steps: preparing carbon dots with different emission wavelengths; carrying out amination modification on the carbon dots; and preparing the dual-emission fluorescent molecularly imprinted polymer. The method also comprises the following steps: respectively adding the dual-emission fluorescent molecularly imprinted polymer into chrysin solutions with different known concentrations, carrying out fluorescence quenching reaction, and establishing a standard curve according to the linear relationship between the detected fluorescence intensity and the corresponding concentration of the chrysin solution; adding the dual-emission fluorescent molecularly imprinted polymer into a solution of a to-be-detected product, and carrying out fluorescence quenching reaction to obtain fluorescence intensity; and calculating the concentration of chrysin in the solution to be detected according to the standard curve. The preparation method is used for solving the technical problems that in the prior art, a single-emission fluorescent molecularly imprinted polymer has defects in the aspects of sensitivity, background fluorescence interference resistance in a complex environment and the like in the detection process.

The invention belongs to the technical field of food safety detection, and particularly relates to a histamine molecular imprinting electrochemical sensor based on a carbon-based nano composite material and a preparation method and application of the histamine molecular imprinting electrochemical sensor. Aiming at the problems of poor selectivity, high detection limit or complicated operation and the like of the existing histamine detection technology, a Co3O4 / N-HCS (cobaltosic oxide / nitrogen-doped hollow carbon sphere) composite material is prepared by adopting a solvothermal method, o-phenylenediamine (o-PD) is taken as a functional monomer, a molecularly imprinted polymer membrane (MIP) is constructed on the surface of a glassy carbon electrode (GCE) by adopting an electropolymerization method, and the histamine detection electrode is prepared. The MIP / Co3O4 / N-HCS / GCE sensor with specific recognition sites on histamine is formed. The sensor has high selectivity, high sensitivity and good stability, is simple and convenient to operate and rapid in detection, can efficiently realize accurate detection of histamine in food, and is suitable for safety monitoring scenes of various foods such as fermented food, aquatic products and dairy products.

The invention discloses a chitosan modified ferroferric oxide / carboxylated multi-walled carbon nanotube-based molecularly imprinted electrochemical sensor as well as a preparation method and application thereof, and relates to the technical field of electrochemical sensing. The invention aims to solve the problems that the existing method for detecting carbendazim is complicated to operate and high in cost and the traditional sensor is poor in selectivity. The invention discloses a molecularly imprinted electrochemical sensor based on chitosan modified ferroferric oxide / carboxylated multi-walled carbon nanotubes. The molecularly imprinted electrochemical sensor comprises a glassy carbon electrode, and a molecularly imprinted polymer and CS-Fe3O4-CMWCNTs which are modified on the surface of the glassy carbon electrode, the molecularly imprinted polymer is prepared by taking carbendazim as a template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent and azodiisobutyronitrile as an initiator. The molecularly imprinted electrochemical sensor is applied to detection of carbendazim.

The invention discloses a molecularly imprinted membrane of a new psychoactive substance and a preparation method and application of the molecularly imprinted membrane, the molecularly imprinted membrane comprises a membrane substrate and an adsorption material layer arranged on the membrane substrate, and the material of the adsorption material layer comprises 3, 3 ', 4', 4 '-tetramethyl-3, 3', 4 '-tetramethyl-3, 3'-tetramethyl-3, 3 '-tetramethyl-3, 3'- The molecularly imprinted polymer is prepared by using 2, 4-methylenedioxyphenylethylamine as an alternative template and using a bulk polymerization method. The novel molecularly imprinted membrane prepared by combining a molecularly imprinted technology and a separation technology is used as a membrane extraction material, and integrates the advantages of a membrane extraction technology and an imprinting technology, so that the problems that a membrane material is easy to pollute and low in selectivity are solved, and the novel molecularly imprinted membrane is suitable for being used as a membrane extraction material. The method shows the capability of accurately identifying a target analyte in the membrane separation process, has the advantages of high efficiency, stability, repeatability and the like, and provides a new technical means and potential application for identifying and detecting common forbidden drugs in a water body.

This invention discloses a dual-emission molecularly imprinted ratiometric fluorescence sensor, its preparation method, and its application in the detection of chlortetracycline, belonging to the field of chemical sensor technology. The preparation method of this sensor includes the following steps: preparing silica-quantum dot composite nanoparticles; preparing a molecularly imprinted polymer using salicylamide as a template molecule; and using a post-mixing strategy to mix the molecularly imprinted polymer and silica-quantum dot composite nanoparticles in HEPES buffer to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The advantages of this invention are: the dual-emission molecularly imprinted ratiometric fluorescence sensor provided by this invention exhibits rich fluorescence color changes, enabling rapid, highly sensitive, and visual detection of chlortetracycline; and the entire detection process is simple, convenient, and fast, making it suitable for widespread application in practical applications.

The invention discloses a visual bifunctional subtype influenza virus molecularly imprinted sensor as well as a preparation method and application thereof. The sensor comprises a fluorescent signal core and a molecularly imprinted polymer layer coated outside the fluorescent signal core, the fluorescent signal core is SiO2 microspheres coated with CdTe quantum dots, the molecularly imprinted polymer layer is internally provided with an imprinting cavity capable of specifically recognizing the subtype influenza virus, the three-dimensional space shape of the imprinting cavity is complementary to that of the subtype influenza virus, and the molecularly imprinted polymer layer is coated with CdTe quantum dots. Sialic acid molecules are covalently modified on the inner surface. The sensor can be used for quantitative detection or visual semi-quantitative detection of subtype influenza viruses such as H7N9 and H5N1, and integrates double functions of rapid screening and accurate quantification. The sensor disclosed by the invention is good in preparation reproducibility, high in selectivity, strong in anti-interference capability and suitable for analysis of complex biological samples, and a novel user-friendly and efficient method is provided for on-site monitoring and early warning of influenza viruses.

The invention relates to the technical field of medical preparations or cosmetics, in particular to the technical field of preparation of plant-derived active ingredients in the medical preparations or cosmetics, and discloses a preparation process of a centella asiatica extract, which comprises the following steps: extracting centella asiatica to obtain a centella asiatica concentrated product from centella asiatica by adopting an enzyme-assisted hot ethanol extraction method in combination with a resin purification technology; the preparation method comprises the following steps: preparing centella asiatica extract molecularly imprinted polymer resin, filling a solid-phase extraction empty column with the centella asiatica extract molecularly imprinted polymer resin to prepare a resin column, and purifying a centella asiatica concentrated product through the resin column to prepare the centella asiatica extract with the purity as high as 99%. The centella asiatica extract prepared by the invention has repairing and soothing effects, can be used as an active ingredient in medical preparations or cosmetics, is used for preparing a gel preparation for treating skin injury, or is added into a repairing skin care product, and realizes the combination of a medical-grade skin care effect and daily care by promoting skin barrier repairing and soothing damaged parts.

This invention discloses a method for preparing and applying a wearable formaldehyde gas sensor based on molecularly imprinted polymers. The preparation method includes the following steps: forming a heart-shaped electrode stepwise on a flexible substrate using screen printing technology; synthesizing a thin layer of polypyrrole-based molecularly imprinted polymer on the working electrode of the heart-shaped electrode using electropolymerization technology; immersing the synthesized electrode in a mixed solution of acetic acid and methanol; and drying to obtain the wearable formaldehyde gas sensor. This method and application of the wearable formaldehyde gas sensor, through a combination of molecular imprinting technology, screen printing, and electropolymerization, prepares a layered structure of "carbon paste-molecularly imprinted polymer" on a flexible substrate. It can rapidly respond to formaldehyde gas at ppm levels at room temperature, maintains good working condition during multiple bending tests, and exhibits extremely strong selectivity for formaldehyde in complex working environments. The preparation process is simple, low-cost, and highly practical.

The invention discloses a method for reducing COD (Chemical Oxygen Demand) of tetrahydrofuran production process wastewater, which comprises the following steps: S1, introducing the tetrahydrofuran production process wastewater into a molecular imprinting adsorption unit to selectively adsorb 1, 4-butanediol in the wastewater; s2, performing in-situ regeneration treatment on the molecularly imprinted polymer; meanwhile, the effluent is conveyed to a Fenton oxidation unit for oxidation treatment; s3, the outlet water is conveyed to an iron removal unit for iron removal operation; s4, introducing the effluent into a photocatalytic membrane separation unit, and carrying out photocatalytic oxidation degradation operation; and S5, sending the effluent to an activated carbon adsorption unit for adsorption treatment, and discharging the effluent after the effluent reaches the discharge standard. According to the wastewater treatment method disclosed by the invention, the 1, 4-butanediol can be efficiently and selectively removed, and the load of a subsequent working section is greatly reduced; and by virtue of the advantages of high-selectivity adsorption, strong oxidation, membrane self-cleaning and the like, the COD (Chemical Oxygen Demand) of the effluent is stably lower than the discharge standard, the whole process is stable in operation, and the long-term wastewater treatment requirement can be met.

The application specifically relates to a targeted cleaning agent composition and a process for cleaning regenerated PET bottle pieces. The targeted cleaning agent composition comprises the following components: a nano-sized molecularly imprinted polymer, which is prepared by taking an acrylate compound or a derivative thereof as a template molecule and has specific recognition and adsorption capacity for the adhesive on the surface of the PET bottle piece; at least one kind of engineered enzyme selected from lipase, esterase and protease; a non-ionic surfactant and deionized water. The application introduces the molecularly imprinted polymer into the field of plastic cleaning, initiates a three-module synergistic mechanism of "recognition-catalysis-stripping", recognizes and enriches the enzyme on the surface of the adhesive layer by the nano-sized MIP (molecularly imprinted polymer), realizes "anchored catalysis", upgrades the "free diffusion" mode of the enzyme into a "targeted enrichment" mode, and the adhesive can be efficiently removed under low-temperature and mild conditions by using the cleaning agent of the application, and the PET molecular chain is maximally protected.

The invention discloses a molecularly imprinted photoelectrochemical sensor for detecting tetracycline and a preparation and detection method, and belongs to the technical field of environmental analysis and detection. The preparation method comprises the following steps: firstly, electrically depositing a BiOI nanosheet on FTO (Fluorine-doped Tin Oxide) conductive glass, and sequentially carrying out vanadium source coating, heat treatment and alkali washing to obtain a high-performance BiVO4 photo-anode; and then the surface of BiVO4 is modified with a CoMn-LDH layer, so that separation and transmission of photo-generated charges are effectively promoted. Finally, tetracycline is taken as a molecular imprinting template, o-phenylenediamine is taken as a functional monomer, electropolymerization is carried out on the surface of the composite electrode, and after elution, the molecular imprinting polymer membrane with specific recognition capability on tetracycline is formed. When the molecularly imprinted polymer is used for detecting tetracycline, a target molecule is specifically combined with an imprinted hole and then is oxidized by a photo-generated hole as an electron donor, so that the separation and migration efficiency of a photo-generated carrier is remarkably enhanced. The method has the advantages of high sensitivity, wide linear range and the like, and high-sensitivity detection of tetracycline residues in an environmental water body can be realized.

The invention concerns the development of molecularly imprinted polymer for label-free capacitive sensing application. The method outlined by the invention involves several steps: a) Pre-treating the surface of the working electrode; b) Electrochemical polymerizing a monomer solution in an electrolyte buffer containing the target template molecule to form an imprinting template film on the electrode surface; c) Washing the electrode to remove unbound molecules; d) Characterizing the formed imprinting template film by cyclic voltammetry in a supporting electrolyte solution to determine its half-wave potential; e) Forming the molecularly imprinted polymer electrode by removing the target template molecule from the synthesized polymer; f) Incubating the obtained molecularly imprinted polymer with the target template for a specified duration; g) Detecting the target template molecule using capacitance-derived electrochemical impedance spectroscopy. This method presents significant advantages in terms of specificity, sensitivity, and stability, rendering it highly applicable across numerous industries for enhancing detection, monitoring, and diagnostic capabilities.

A layer structure having a solid substrate having a surface, the surface carrying a layer including a molecularly imprinted polymer, wherein the molecularly imprinted polymer is adapted to bind a glyphosate analyte, wherein the glyphosate analyte is selected from glyphosate, a glyphosate degradation product, a metabolite of glyphosate or a metabolite of the degradation product of glyphosate, wherein a fluorescence characteristic of the molecularly imprinted polymer changes upon binding of the glyphosate analyte. Further, a method for detecting a glyphosate analyte in a sample includes: providing the layer structure having the molecularly imprinted polymer; providing a fluidic contact of the layer structure with the sample or an organic extract of the sample; measuring a fluorescence property of the layer structure; and estimating a concentration of glyphosate or of the glyphosate related analyte at least semi-quantitatively.

The invention relates to the technical field of liquid chromatography / mass spectrometry analysis, and discloses a method for improving LC / MS analysis sensitivity, which is suitable for detecting trace lipid metabolites (containing isomeride) of a biological sample. The core technical scheme is as follows: a magnetic molecularly imprinted polymer-solid phase support liquid-liquid extraction secondary purification system is adopted to remove matrix impurities, a gradient mobile phase containing a composite additive is matched to optimize separation, and the detection specificity is improved by an ion mobility spectrometry-segmented selective ion monitoring combined mode; and a stable isotope internal standard is synchronously introduced to correct quantitative deviation. Experiments show that the LOD of the method is as low as 0.12-0.25 ng / mL, the matrix effect is smaller than or equal to 5%, the separation degree and the recovery rate are excellent, low-abundance metabolic marker differences can be accurately captured, and a support is provided for disease diagnosis and metabonomics research.

Various biosensors for detecting biomolecules, biosensing devices including the biosensors and systems including the biosensing devices are described herein. The biosensors include a first electrode and a second electrode, and a piezoelectric layer placed between the first electrode and the second electrode. The first electrode includes one or more regions coated with one or more molecularly imprinted polymer (MIP) coatings, each configured to bind a molecule of interest. The biosensor is configured to detect a concentration of each of the molecules of interest by determining a resonant frequency of the piezoelectric layer when the one or more regions of the first electrode are in contact with a solution. The biosensor has a microwave-based configuration or an acoustic wave-based configuration.

The invention discloses a molecular imprinting separation method for gallic acid in tara enzymolysis tannic acid waste liquid. The molecular imprinting separation method comprises the following steps: (1) preparing Fe3O4 nanoparticles; (2) preparing Fe3O4 (at) SiO2 nano particles; (3) preparing Fe3O4 (at) SiO2 (at) NH2 nano particles; (4) preparing a Fe3O4 (at) SiO2 (at) NH2 molecularly imprinted polymer; (5) pretreatment of waste liquid; and (6) gallic acid separation. The method has obvious advantages in the aspects of operation convenience, separation selectivity and repeatability, and an efficient and feasible technical scheme is provided for the field of natural product extraction.

The present invention belongs to the technical field of analytical chemistry. Specifically disclosed are a magnetic fluorescent gold nanocluster molecularly imprinted polymer, a preparation method therefor, and use thereof in sulfadiazine fluorescence detection. The surface of a Fe3O4 nanoparticle is coated with a silica shell layer, and the shell layer is subjected to surface modification and then combined with gold nanoclusters (AuNCs). On this basis, the molecular imprinting technology is employed, wherein sulfadiazine (SD) is selected to serve as a template molecule, and a mixed silane coupling agent (APTES) is used as a functional monomer to coat the surface of Fe3O4@SiO2@AuNCs with a molecular imprinting layer, thus preparing a magnetic fluorescent gold nanocluster imprinted polymer Fe3O4@SiO2@AuNCs-MIPs. The present invention, by integrating the magnetic separation technology, the high selectivity, simplicity, and rapidity of the molecularly imprinted polymer, and the high sensitivity of fluorescence detection, enables accurate and specific recognition of sulfadiazine and can be effectively applied to the analysis and detection of sulfadiazine.

To resolve the problem of reduced sensor sensitivity caused by silicone oil in a molecularly imprinted polymer paste, in a sensor that includes an electrode immobilized with the paste. [Solution] A sensor that utilizes conductive carbon for wiring, wherein the sensor has (a) an working electrode, (b) a counter electrode, (c) a reference electrode, (d) a wiring section, and (e) a connection section to a measuring instrument, the working electrode is formed from a molecular imprint polymer paste containing conductive particles having molecular imprint polymers on their surface and silicone oil, and a portion of the wiring section between the connection section to the measuring instrument and the working electrode is replaced with a conductive and non-lipophilic substance. A sensor comprising an electrode substrate coated or filled with a molecular imprint polymer paste containing conductive particles having molecular imprint polymers on their surface and an amphiphilic substance.

The invention provides a preparation method and application of a molecularly imprinted surface plasmon resonance (SPR) sensing chip of ciprofloxacin (CFX). According to the chip, molecularly imprinted polymers (MIPs) are combined with an SPR technology, so that the detection sensitivity and accuracy are effectively improved, the chip has obvious signal response to CFX with lower concentration, and the detection limit can be as low as nanomole level. When the CFX molecularly imprinted SPR chip prepared by the invention is used for CFX detection, the operation steps are simple, the analysis time is short, the specificity and reproducibility are good, the operation is simple and rapid, and the chip has important practical application value.

The invention relates to the technical field of nano biosensors, and discloses a bimodal nano probe for uric acid detection as well as a preparation method and application of the bimodal nano probe. The bimodal nano-probe is a fluorescent-photo-thermal bimodal nano-probe, is a core-shell structure nano-particle, and sequentially comprises the following components from the inner layer to the outer layer: a) a fluorescent core which is composed of a carbon dot and a silicon dioxide layer coating the carbon dot; b) a manganese dioxide layer coated outside the fluorescent inner core; and c) a molecularly imprinted polymer layer coated outside the manganese dioxide layer. According to the bimodal nanoprobe disclosed by the invention, through ingenious structural design, a fluorescence signal source (carbon dots), a signal modulation and photo-thermal unit and a specific recognition layer (uric acid molecularly imprinted polymer) are integrated, so that bimodal sensing of'specific recognition-> MnO2 etching-> fluorescence recovery / photo-thermal weakening 'on UA is realized; the problems of poor selectivity, complicated operation and high cost of a traditional method are effectively solved.

The invention provides a molecularly imprinted polymer membrane based on microfluidic confinement and a preparation method and application thereof, the preparation method comprises the following steps: taking a base material, and arranging a microchannel on the base material; adding the prepolymerization solution into the microchannel; the prepolymerization solution comprises a silane coupling agent, template molecules, a cross-linking agent and a catalyst; and carrying out polymerization reaction on the prepolymerization solution under a confinement condition to prepare the molecularly imprinted polymer membrane. The microchannel is used for providing a confinement environment for in-situ growth of the molecularly imprinted polymer film, simultaneous imprinting of multiple template molecules is achieved, the obtained molecularly imprinted polymer film is uniform and controllable in thickness and matched with a detection probe, simultaneous detection of multiple substances can be achieved, the preparation method is simple, and preparation conditions are mild. According to the invention, high-integration-level detection of MIPs and NIPs is realized on the same chip, and the chip is simultaneously applied to simultaneous identification, separation, enrichment and detection of simultaneous detection of various substances.