45results about How to "Strong specificity recognition ability" patented technology

The invention discloses a cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and a method for preparing the cell probe complex. The cell probe complex is characterized in that nucleic acid aptamer capable of realizing a targeting effect with the MCF-7 cells is used as a template, bimetal alloy nano particles of gold and silver are deposited on a basic group of a DNA (deoxyribose nucleic acid) chain of the nucleic acid aptamer by a photocatalysis method, and Raman signal molecules rhodamine 6G (Rh6G) is fixedly loaded on the surface of each nano particle. The structure of the probe complex is chain-shaped as shown by a photo of a transmission electron microscope, and the probe complex is capable of realizing the targeting effect with the MCF-7 cells. Characteristic absorption peaks of the rhodamine 6G appear on the Raman spectrum under the effect of the MCF-7 cells and the probe complex, and the human breast cancer MCF-7 cells can be effectively, sensitively and specifically detected.

The invention relates to a spirit identifying system based on volatizing control and a visualized array sensor. The system comprises a volatizing device, a detecting device, a visualized array sensor and an image collecting instrument, wherein the volatizing device consists of an inner layer I and an outer layer I; the detecting device consists of an inner layer II and an outer layer II; the inner layer I is a spirit storing cavity; a heating cavity I is arranged between the inner layer I and the outer layer I; the inner layer II is a spirit gas detecting cavity; a heating cavity II is arranged between the inner layer II and the outer layer II; the visualized array sensor is arranged in the spirit gas detecting cavity; and the image collecting instrument is arranged at the top of the spirit gas detecting cavity. The spirit is volatized by the volatizing control device under the required condition and comes into contact with the visualized array sensor in the input detecting device, the signal responded by the feature of the sample to be detected is detected by the visualized array sensor, the spectrum change signal collected by the image collecting instrument is input to a signal identifying and processing system, the detecting results are displayed in a spectrogram way and then the visualized detection is realized. The detection is convenient and rapid by adopting the system.

The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.

The invention provides application of a cholesterol molecule imprinted membrane sensing electrode in detecting cholesterol content in blood. Specific steps in the method are as follows: (1) treating a blood sample, that is, standing the blood sample at room temperature and carrying out centrifugation to extract serum if the blood sample is obtained by drawing blood from a person who is on an empty stomach, or carrying out anticoagulation by using heparin or EDTA (ethylene diamine tetraacetic acid) if the blood sample is blood plasma; (2) dropwise adding the blood sample obtained in step (1) onto the cholesterol molecule imprinted membrane sensing electrode, inserting the cholesterol molecule imprinted membrane sensing electrode into an amperometric sensor for determination, and recording readings. When used for detecting cholesterol content in blood, the molecule imprinted membrane sensing electrode has the advantages of low cost, high sensitivity, fast analysis speed, capacity of reducing sampling amount and avoiding tedious pretreatment of blood samples, etc.

The invention relates to a folacin/biotin modified chitosan material and a preparation method thereof. The folacin/biotin modified chitosan material is prepared by carrying out N-acylation reaction, wherein chitosan servers as a carrier, and folacin and biotin serve as ligands. Because an active biotin ester and an active folacin ester are added in sequence to react with chitosan for one time to synthesize a double-ligand modified chitosan material, a high grafting ratio can be achieved, and the time of reaction can be shortened greatly compared with the time of reaction in two steps. The prepared folacin/biotin modified chitosan material can be used as a targeted anticancer medicine carrying material.

The invention belongs to the field of molecular imprinting sensor field, and more specifically relates to a method used for photocatalysis RAFT polymerization preparation of a molecular imprinting sensor. The method mainly comprises following steps: a compound containing a tertiary amine structure is taken as a template molecule, and a template molecule-functional monomer pre-polymerized solutionis synthesized; tris (bipyridine) ruthenium is taken as both as a photocatalyst and an electrochemiluminescence agent, photocatalysis RAFT polymerization is carried out, and synthesis of a molecular imprinting polymer with a structure and a size similar to those of the template molecule is realized; and at last, a molecular imprinting sensor is obtained. The molecular imprinting sensor is high inspecific identifying capacity, can be used for quantitative determination, and the lowest detection lower limit concentration reaches 1*10<-13>mol/L. According to the preparation method, no extra polymerization initiator is needed, the process is more simple, is high in efficiency, is green, and is friendly to the environment.

The invention provides a method for preparing dexketoprofen. The method comprises deamination of ketoprofen amide catalyzed by amidase derived from Klebsiella to obtain dexketoprofen. The inventors found that the amidase derived from Klebsiella has stronger specific recognition ability to dexketoprofen, and the dexketoprofen is prepared by using the amidase derived from Klebsiella, so that the eevalue of dexketoprofen can be greatly.

The invention discloses a preparation method and application of a molecularly imprinted photonic crystal for detecting sulfamethoxazole. The preparation method comprises the following steps of firstly, depositing polystyrene emulsion microspheres on the surface of a glass slide by utilizing a vertical deposition method to obtain a photonic crystal template, filling gaps of the polystyrene microspheres on the photonic crystal template with a precursor solution containing sulfamethoxazole, a functional monomer, a cross-linking agent and an initiator, carrying out a constant temperature reaction for a period of time, and eluting the polystyrene microspheres and sulfamethoxazole with a solvent to prepare the molecularly imprinted photonic crystal. The molecularly imprinted photonic crystal provided by the invention shows different colors in sulfamethoxazole solutions with different concentrations, and the concentration range of sulfamethoxazole can be roughly judged through naked eye observation. Furthermore, the displacement of the Bragg diffraction peak of the photonic crystal is measured by using a fiber optic spectrometer, so that quantitative analysis on trace sulfamethoxazole can be realized.

The invention relates to a conductive type sensing electrode for detecting chloramphenicol in the field of biosensors and a method for preparing a molecular engram membrane of the chloramphenicol. In the invention, the conductive type sensing electrode is formed by arranging two screen painting electrodes in parallel. The method for preparing the molecular engram membrane of the chloramphenicol comprises the following steps: template molecules are embedded in a direct in-situ polymerization process of polymer fluid on the surfaces of the disposable screen painting electrodes, and then the template molecules are eluted so that cavities with set sizes and shapes and functional groups in confirmed arrangement are formed in the molecular engram membrane and have memorability to stereoscopic structures of the template molecules. Chloramphenicol molecules in a sample to be detected can be combined with the specificity of the cavities so as to change the electrochemical properties of the molecular engram membrane, and increase the conductivity and the membrane conductance of the molecular engram membrane. The processing method of the invention has simplicity, convenience and low cost, and the conductive type sensing electrode made of the screen painting electrodes has convenient operation, high response time and long service life when used for detecting the chloramphenicol.

The invention discloses a preparation method of a shrimp shell based magnetic hierarchical porous carbon surface imprinting material. The preparation method comprises following steps: shrimp shell active raw material preparation, shrimp shell based magnetic hierarchical porous carbon preparation, modified shrimp shell based magnetic hierarchical porous carbon preparation, and shrimp shell based magnetic hierarchical porous carbon surface imprinting material preparation. The prepared shrimp shell based magnetic hierarchical porous carbon surface imprinting material can be used in selective identification and separation of phenolic endocrine disruptors in waste water, is high in selectivity and adsorption capacity, and can be regenerated.

The invention discloses a p53 protein monoclonal antibody and application thereof. The invention discloses an immunogen fragment of a p53 protein monoclonal antibody. The fragment is a p53 protein 1-80 region; a corresponding coating antigen peptide fragment screened by ELISA is a p53 protein 15-39 region, and one more cysteine is synthesized at the N end of the polypeptide. The cloning number of the monoclonal cell strain is 4H12E8, the subtype of a secreted antibody is IgG1, and the cell strain can secrete a p53 protein monoclonal antibody. Compared with an immunogen and a coating antigen, the p53 protein monoclonal antibody disclosed by the invention has the same peptide fragment, the prepared p53 protein monoclonal antibody has stronger specific recognition effect, can recognize p53 proteins in various samples, and has stronger positive signal and superior positive rate compared with a commercially common p53 protein monoclonal antibody; the method is suitable for detecting p53 protein in various tumor samples.

The invention discloses a preparation method and application of a molecularly imprinted photonic crystal for detecting sulphaguanidine. The preparation method comprises the following steps: firstly, uniformly depositing polystyrene microspheres with a certain size on the surface of a glass slide by utilizing a vertical deposition method to form a photonic crystal template; then filling gaps of polystyrene microspheres of the photonic crystal template with a precursor solution containing imprinted molecules, a functional monomer, a cross-linking agent and an initiator under the action of capillary force, and after a thermal initiation polymerization reaction, removing the polystyrene microspheres and the imprinted molecules in sequence by using an eluent to obtain the molecularly imprinted photonic crystal with an inverse opal structure. The obtained molecularly imprinted photonic crystal shows different structural colors when placed in sulphaguanidine solutions with different concentrations, the concentration range of the sulphaguanidine solutions can be judged through naked eye observation, the Bragg diffraction peak displacement of the molecularly imprinted photonic crystal is further measured through a fiber optic spectrometer, and quantitative measurement of trace sulphaguanidine can be achieved.

The invention relates to a preparation method for an integrated POSS and MCM-41-MPS doped capecitabine molecularly-imprinted sustained-release or controlled-release material, specifically to the preparation method and performance evaluation of a capecitabine molecularly-imprinted sustained-release or controlled-release material doped by POSS and the mesoporous molecular sieve MCM-41. Results showthat due to the existence of both POSS and MCM-41-MPS, the selective recognition ability and adsorption performance of a molecularly-imprinted polymer can be significantly improved, better imprintingeffect is generated, and certain influence is performed on the specific surface area and pore size distribution of the polymer. Hybrid MIP is used as a carrier material of capecitabine, and in-vitro and in-vivo drug release experiment results prove that an optimal formula is significantly prolonged in drug release time and significantly improved in relative bioavailability (wherein F is 173.4%), so great research significance and practical value are achieved when the hybrid MIP is applied to the sustained-release or controlled-release material of the capecitabine.