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Molecularly imprinted polymer and preparation method and application thereof
InactiveCN101864029AStrong specificity recognition abilityImprove solubilityComponent separationCross-linkPolymer science
The invention discloses a molecularly imprinted polymer and a preparation method and application thereof. The molecularly imprinted polymer of the invention is the molecularly imprinted polymer, which is prepared by using ractopamine as a template, polymerizing the template with a functional monomer, a cross-linking agent, a pore-forming agent and an initiator to form a block polymer, grinding the block polymer, sieving the obtained powder, removing the template by using acetate methoxide and methoxide and carrying out vacuum drying on the obtained product and has high compatibility and obvious selectivity for the ractopamine. The molecularly imprinted polymer of the invention has high compatibility and selectivity for the ractopamine and has wide application prospect in analysis of a sample pretreatment solid phase extraction material of the ractopamine in substrates such as a feed, animal tissue or environment water and the like.
Owner:SOUTH CHINA AGRI UNIV
Specific detection method of human breast cancer cells MCF-7 based on surface-enhanced Raman spectroscopy
InactiveCN102608102AStrong specificityStrong specificity recognition abilityRaman scatteringSignalling moleculesSurface-enhanced Raman spectroscopy
The invention relates to a specific detection method of human breast cancer cells MCF-7 based on surface-enhanced Raman spectroscopy. MCF-7 cells act with a probe compound Rh6G-Au-Ag-aptamer, a characteristic absorption peak of Rh6G appears on the obtained Raman spectrum, and MCF-7 cells are detected by surface-enhanced Raman spectroscopy. The probe compound is prepared by the steps of using nucleic acid apatamer S2.2 as a template, depositing gold and silver bimetal alloy nanoparticles on the bases of the DNA chain of the nucleic acid apatamer S2.2 by using a photocatalysis method, and carrying a Raman signaling molecule Rh6G on the surface of the nucleic acid apatamer S2.2. The method is based on the probe compound which is in target binding with the MCF-7 cells and has high surface-enhanced Raman activity, and carries out specific detection of MCF-7 cells by using the specific target binding capacity of the apatamer with the MCF-7 cells and the enhancement effect of the alloy nanoparticles on the surface of the apatamer on the Raman signal of the Rh6G molecule.
Owner:NANJING NORMAL UNIVERSITY
Cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and method for preparing cell probe complex
InactiveCN102621125AHigh Surface Enhanced Raman ActivityImprove specific recognition abilityRaman scatteringDNA/RNA fragmentationSignalling moleculesPhotocatalysis
The invention discloses a cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and a method for preparing the cell probe complex. The cell probe complex is characterized in that nucleic acid aptamer capable of realizing a targeting effect with the MCF-7 cells is used as a template, bimetal alloy nano particles of gold and silver are deposited on a basic group of a DNA (deoxyribose nucleic acid) chain of the nucleic acid aptamer by a photocatalysis method, and Raman signal molecules rhodamine 6G (Rh6G) is fixedly loaded on the surface of each nano particle. The structure of the probe complex is chain-shaped as shown by a photo of a transmission electron microscope, and the probe complex is capable of realizing the targeting effect with the MCF-7 cells. Characteristic absorption peaks of the rhodamine 6G appear on the Raman spectrum under the effect of the MCF-7 cells and the probe complex, and the human breast cancer MCF-7 cells can be effectively, sensitively and specifically detected.
Owner:NANJING NORMAL UNIVERSITY
Spirit identifying system based on volatizing control and visualized array sensor
InactiveCN101858854AVolatile stabilityImprove stabilityColor/spectral properties measurementsEngineeringHandling system
The invention relates to a spirit identifying system based on volatizing control and a visualized array sensor. The system comprises a volatizing device, a detecting device, a visualized array sensor and an image collecting instrument, wherein the volatizing device consists of an inner layer I and an outer layer I; the detecting device consists of an inner layer II and an outer layer II; the inner layer I is a spirit storing cavity; a heating cavity I is arranged between the inner layer I and the outer layer I; the inner layer II is a spirit gas detecting cavity; a heating cavity II is arranged between the inner layer II and the outer layer II; the visualized array sensor is arranged in the spirit gas detecting cavity; and the image collecting instrument is arranged at the top of the spirit gas detecting cavity. The spirit is volatized by the volatizing control device under the required condition and comes into contact with the visualized array sensor in the input detecting device, the signal responded by the feature of the sample to be detected is detected by the visualized array sensor, the spectrum change signal collected by the image collecting instrument is input to a signal identifying and processing system, the detecting results are displayed in a spectrogram way and then the visualized detection is realized. The detection is convenient and rapid by adopting the system.
Owner:CHONGQING UNIV
Molecular imprinting polymer for roxarsone detection, and preparation method thereof
InactiveCN103232572AStrong specificity recognition abilityImprove solubilityOther chemical processesPreparing sample for investigationPolymer scienceFunctional monomer
The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.
Owner:SOUTH CHINA AGRI UNIV
Application of cholesterol molecule imprinted membrane sensing electrode in detecting blood cholesterol
InactiveCN102353710ALower requirementGood flexibilityMaterial analysis by electric/magnetic meansEthylene diamine tetra aceticEthylene diamine
The invention provides application of a cholesterol molecule imprinted membrane sensing electrode in detecting cholesterol content in blood. Specific steps in the method are as follows: (1) treating a blood sample, that is, standing the blood sample at room temperature and carrying out centrifugation to extract serum if the blood sample is obtained by drawing blood from a person who is on an empty stomach, or carrying out anticoagulation by using heparin or EDTA (ethylene diamine tetraacetic acid) if the blood sample is blood plasma; (2) dropwise adding the blood sample obtained in step (1) onto the cholesterol molecule imprinted membrane sensing electrode, inserting the cholesterol molecule imprinted membrane sensing electrode into an amperometric sensor for determination, and recording readings. When used for detecting cholesterol content in blood, the molecule imprinted membrane sensing electrode has the advantages of low cost, high sensitivity, fast analysis speed, capacity of reducing sampling amount and avoiding tedious pretreatment of blood samples, etc.
Owner:无锡市金坤生物工程有限公司
Folacin/biotin modified chitosan material and preparation method thereof
InactiveCN104558246AHigh grafting rateShort reaction timePharmaceutical non-active ingredientsChemistryFolic acid
The invention relates to a folacin / biotin modified chitosan material and a preparation method thereof. The folacin / biotin modified chitosan material is prepared by carrying out N-acylation reaction, wherein chitosan servers as a carrier, and folacin and biotin serve as ligands. Because an active biotin ester and an active folacin ester are added in sequence to react with chitosan for one time to synthesize a double-ligand modified chitosan material, a high grafting ratio can be achieved, and the time of reaction can be shortened greatly compared with the time of reaction in two steps. The prepared folacin / biotin modified chitosan material can be used as a targeted anticancer medicine carrying material.
Owner:WUHAN UNIV OF TECH
Azithromycin detection molecular imprinting monolithic micro column and preparation method thereof
InactiveCN104829774AImprove solubilityImprove liquid permeabilityComponent separationFunctional monomerMicro column
The invention discloses an azithromycin detection molecular imprinting monolithic micro column and a preparation method thereof, and belongs to the technical field of material chemistry. The preparation method of the azithromycin detection molecular imprinting monolithic micro column comprises the following steps: a template, a mixed pore forming agent, a functional monomer, a crosslinking agent and an initiator are polymerized at 50-80 DEG C for 48h, and the template is removed to obtain the azithromycin detection molecular imprinting monolithic micro column. The azithromycin recovery rate of the prepared molecular imprinting monolithic micro column is more than 90%, the prepared molecular imprinting monolithic micro column shows high cross reactivity, high selectivity and specificity, and has a broad application prospect in use as a purification and pretreatment material for analysis of a sample of azithromycin in animal tissues, feeds and environmental water, and other matrix.
Owner:SOUTH CHINA AGRI UNIV
Method used for photocatalysis RAFT polymerization preparation of molecular imprinting sensor, and applications thereof
ActiveCN108918483AAdvantages and Notable ImprovementsSignificant progressFluorescence/phosphorescenceMaterial electrochemical variablesFunctional monomerElectrochemiluminescence
The invention belongs to the field of molecular imprinting sensor field, and more specifically relates to a method used for photocatalysis RAFT polymerization preparation of a molecular imprinting sensor. The method mainly comprises following steps: a compound containing a tertiary amine structure is taken as a template molecule, and a template molecule-functional monomer pre-polymerized solutionis synthesized; tris (bipyridine) ruthenium is taken as both as a photocatalyst and an electrochemiluminescence agent, photocatalysis RAFT polymerization is carried out, and synthesis of a molecular imprinting polymer with a structure and a size similar to those of the template molecule is realized; and at last, a molecular imprinting sensor is obtained. The molecular imprinting sensor is high inspecific identifying capacity, can be used for quantitative determination, and the lowest detection lower limit concentration reaches 1*10<-13>mol / L. According to the preparation method, no extra polymerization initiator is needed, the process is more simple, is high in efficiency, is green, and is friendly to the environment.
Owner:QINGDAO UNIV
Nucleic acid dye compound and preparation method and application of nucleic acid dye compound
ActiveCN108165045AHigh selectivityLow biological toxicityStyryl dyesMethine/polymethine dyesMembrane permeabilityFluorescence
The invention provides a nucleic acid dye compound and a preparation method and application of the nucleic acid dye compound. The provided compound, as a fluorescence probe, can selectively recognizeG-quadruplex DNA and RNA; compared with the fluorescence probe having similar functions, the probe has the characteristics of strong selectivity, low bio-toxicity, good membrane permeability, strong developing, good counterstaining compatibility, and stronger light stability, and the characteristics indicate that the compound has extensive applications as the G-quadruplex DNA and the nucleolus fluorescence probe, and is expected to be developed into a simple convenient intuitive biological detection agent for the related physiological and pathological studies of the G-quadruplex DNA and nucleolus.
Owner:GUANGDONG UNIV OF TECH
Method for preparing dexketoprofen
InactiveCN111363736AImprove catalytic reaction efficiencyStrong specificity recognition abilityHydrolasesMicroorganism based processesCombinatorial chemistryAmidase
The invention provides a method for preparing dexketoprofen. The method comprises deamination of ketoprofen amide catalyzed by amidase derived from Klebsiella to obtain dexketoprofen. The inventors found that the amidase derived from Klebsiella has stronger specific recognition ability to dexketoprofen, and the dexketoprofen is prepared by using the amidase derived from Klebsiella, so that the eevalue of dexketoprofen can be greatly.
Owner:HEC PHARM
Preparation method and application of molecularly imprinted photonic crystal for detecting sulfamethoxazole
InactiveCN112964672ALarge specific surface areaStrong specificity recognition abilityMaterial analysis by observing effect on chemical indicatorScattering properties measurementsFunctional monomerMicrosphere
The invention discloses a preparation method and application of a molecularly imprinted photonic crystal for detecting sulfamethoxazole. The preparation method comprises the following steps of firstly, depositing polystyrene emulsion microspheres on the surface of a glass slide by utilizing a vertical deposition method to obtain a photonic crystal template, filling gaps of the polystyrene microspheres on the photonic crystal template with a precursor solution containing sulfamethoxazole, a functional monomer, a cross-linking agent and an initiator, carrying out a constant temperature reaction for a period of time, and eluting the polystyrene microspheres and sulfamethoxazole with a solvent to prepare the molecularly imprinted photonic crystal. The molecularly imprinted photonic crystal provided by the invention shows different colors in sulfamethoxazole solutions with different concentrations, and the concentration range of sulfamethoxazole can be roughly judged through naked eye observation. Furthermore, the displacement of the Bragg diffraction peak of the photonic crystal is measured by using a fiber optic spectrometer, so that quantitative analysis on trace sulfamethoxazole can be realized.
Owner:TIANJIN UNIV
Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof
InactiveCN101424650ASimple structureHigh sensitivityMaterial impedanceIn situ polymerizationElectric conductance
The invention relates to a conductive type sensing electrode for detecting chloramphenicol in the field of biosensors and a method for preparing a molecular engram membrane of the chloramphenicol. In the invention, the conductive type sensing electrode is formed by arranging two screen painting electrodes in parallel. The method for preparing the molecular engram membrane of the chloramphenicol comprises the following steps: template molecules are embedded in a direct in-situ polymerization process of polymer fluid on the surfaces of the disposable screen painting electrodes, and then the template molecules are eluted so that cavities with set sizes and shapes and functional groups in confirmed arrangement are formed in the molecular engram membrane and have memorability to stereoscopic structures of the template molecules. Chloramphenicol molecules in a sample to be detected can be combined with the specificity of the cavities so as to change the electrochemical properties of the molecular engram membrane, and increase the conductivity and the membrane conductance of the molecular engram membrane. The processing method of the invention has simplicity, convenience and low cost, and the conductive type sensing electrode made of the screen painting electrodes has convenient operation, high response time and long service life when used for detecting the chloramphenicol.
Owner:SHANGHAI JIAO TONG UNIV
Magnetic nanocomposite as well as preparation method and application thereof
ActiveCN105582878AGood magnetic responseStrong specificity recognition abilityIon-exchange process apparatusOther chemical processesChemical separationNanocomposite
The invention relates to a magnetic nanocomposite as well as a preparation method and an application thereof. The magnetic nanocomposite is characterized by being modified with a Fe2O3 layer with a hydrothermal method and is regular in morphology and uniform in distribution. The magnetic nanocomposite has good magnetic responsibility and specific recognition capacity, can be applied to various fields such as a medicine purification system, extraction and separation of effective components, food processing, cosmetics, chemical separation and analysis and the like, can be particularly applied to a complicated component system such as Chinese herbal medicines and the like and has potential industrial production and application values.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Molecular imprinting polymer for roxarsone detection, and preparation method thereof
InactiveCN103232572BStrong specificity recognition abilityImprove solubilityOther chemical processesPreparing sample for investigationPolymer scienceFunctional monomer
The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.
Owner:SOUTH CHINA AGRI UNIV
Water-soluble hydrogen sulfide fluorescent probe, preparation method thereof and application thereof in detection of water sulfide and cell hydrogen sulfide
ActiveCN110156858ALow detection limitGood biocompatibilitySugar derivativesFluorescence/phosphorescenceSolubilityFluorescence
The invention discloses a water-soluble hydrogen sulfide fluorescent probe, a preparation method thereof, application of the water-soluble hydrogen sulfide fluorescent probe in water sulfide detectionand application of the water-soluble hydrogen sulfide fluorescent probe in cell hydrogen sulfide detection. The water-soluble hydrogen sulfide fluorescent probe has a structural formula as shown in aformula VI, wherein the substance in the formula VI is glucose, or galactose, or allose, or altrose, or mannose, or talose. The water-soluble hydrogen sulfide fluorescent probe has the advantages ofgood water solubility, low detection limit, high detection sensitivity and the like. The fluorescent probe is successfully applied to water sulfide detection, has good water sulfide detection performance, and achieves a good detection effect. The fluorescent probe is also successfully applied to the detection of cell hydrogen sulfide, and has a good detection effect.
Owner:SHAOXING UNIVERSITY
A kind of magnetic nanocomposite material and its preparation method and application
ActiveCN105582878BGood magnetic responseStrong specificity recognition abilityIon-exchange process apparatusOther chemical processesChemical separationNanometre
The invention relates to a magnetic nanocomposite as well as a preparation method and an application thereof. The magnetic nanocomposite is characterized by being modified with a Fe2O3 layer with a hydrothermal method and is regular in morphology and uniform in distribution. The magnetic nanocomposite has good magnetic responsibility and specific recognition capacity, can be applied to various fields such as a medicine purification system, extraction and separation of effective components, food processing, cosmetics, chemical separation and analysis and the like, can be particularly applied to a complicated component system such as Chinese herbal medicines and the like and has potential industrial production and application values.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Preparation method of capecitabine molecularly imprinted sustained and controlled release material doped with poss and mcm-41-mps
ActiveCN108939082BIncrease the areaImprove adsorption capacityOrganic active ingredientsInorganic non-active ingredientsMolecular sieveDrug release
The invention relates to a preparation method for an integrated POSS and MCM-41-MPS doped capecitabine molecularly-imprinted sustained-release or controlled-release material, specifically to the preparation method and performance evaluation of a capecitabine molecularly-imprinted sustained-release or controlled-release material doped by POSS and the mesoporous molecular sieve MCM-41. Results showthat due to the existence of both POSS and MCM-41-MPS, the selective recognition ability and adsorption performance of a molecularly-imprinted polymer can be significantly improved, better imprintingeffect is generated, and certain influence is performed on the specific surface area and pore size distribution of the polymer. Hybrid MIP is used as a carrier material of capecitabine, and in-vitro and in-vivo drug release experiment results prove that an optimal formula is significantly prolonged in drug release time and significantly improved in relative bioavailability (wherein F is 173.4%), so great research significance and practical value are achieved when the hybrid MIP is applied to the sustained-release or controlled-release material of the capecitabine.
Owner:TIANJIN MEDICAL UNIV
Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof
InactiveCN101424650BSimple structureHigh sensitivityMaterial impedanceIn situ polymerizationMolecular imprinting
Owner:SHANGHAI JIAOTONG UNIV
A competition chemiluminescence sensor based on quartz optical fiber-ZIF-8-aptamer interface and a preparation method and application thereof
This invention discloses a competitive chemiluminescence sensor based on a quartz fiber-ZIF-8-aptamer interface, its preparation method, and its application in the detection of aflatoxin B1, belonging to the field of food safety detection and chemiluminescence sensing technology. The invention first involves ultrasonic cleaning of the quartz fiber with deionized water, oxygen plasma activation, and APTES silanization treatment to introduce amino groups onto the fiber surface. Then, carboxylated ZIF-8 is chemically covalently fixed using EDC / NHS to form a high specific surface area three-dimensional micro / nano interface. Next, the remaining carboxyl groups on the ZIF-8 surface are reactivated, and the AFB1 amino aptamer is covalently coupled to the ZIF-8-fiber interface. After BSA sealing, a competitive chemiluminescence sensing probe is constructed. This invention has advantages such as high sensitivity, strong specificity, low cost, and simple operation, and can be widely used for rapid screening of aflatoxin B1 and other mycotoxins in grains and foods.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
Method for identifying authenticity of wheat flour based on fusion of raman spectrum and near infrared spectrum
PendingCN122508494AStrong complementarityOvercoming the problem of low-concentration features being submerged
This invention provides a method for identifying the authenticity of wheat flour based on the fusion of Raman and near-infrared spectroscopy, belonging to the field of wheat flour authenticity identification technology. The method includes: acquiring Raman and near-infrared spectral data of the wheat flour sample to be tested, and preprocessing them separately; constructing and training a fusion detection model, which includes a data-level fusion module, a feature-level fusion module, and a decision-level fusion module; the data-level fusion module generates full-spectrum fusion data; the feature-level fusion module concatenates core features into a comprehensive feature set; the decision-level fusion module inputs the comprehensive feature set into a hybrid model; and the trained fusion detection model processes the comprehensive feature set to output the authenticity identification result of the wheat flour sample to be tested. This invention, through a three-level spectral fusion architecture and a dynamic adaptive adversarial mechanism, can effectively achieve high-precision and high-robust identification of wheat flour authenticity.
Owner:阿拉山口海关技术中心 +1
Shrimp shell based magnetic hierarchical porous carbon surface imprinting material, and preparation method and applications thereof
PendingCN110327898APromote hydrolysisPromote dehydrationOther chemical processesWater contaminantsMaterials preparationPerturbateurs endocriniens
The invention discloses a preparation method of a shrimp shell based magnetic hierarchical porous carbon surface imprinting material. The preparation method comprises following steps: shrimp shell active raw material preparation, shrimp shell based magnetic hierarchical porous carbon preparation, modified shrimp shell based magnetic hierarchical porous carbon preparation, and shrimp shell based magnetic hierarchical porous carbon surface imprinting material preparation. The prepared shrimp shell based magnetic hierarchical porous carbon surface imprinting material can be used in selective identification and separation of phenolic endocrine disruptors in waste water, is high in selectivity and adsorption capacity, and can be regenerated.
Owner:HENAN UNIV OF URBAN CONSTR
An electrochemical sensor for measuring 2,4,6-trichlorophenol and its preparation and application
ActiveCN108732218BHigh sensitivityImprove conductivityMaterial electrochemical variablesPolymer compositesElectrochemistry
The invention belongs to the field of molecular imprinting detection, and in particular relates to an electrochemical sensor for measuring 2,4,6-trichlorophenol based on molecular imprinting technology and its preparation and application. In the present invention's electrochemical analysis technology for existing 2,4,6-trichlorophenol, 2,4,6-trichlorophenol and its structural analogues have similar oxidation potentials, resulting in insufficient specific recognition of the target good. The electrochemical sensor prepared in the present invention is based on molecular imprinting technology and can be used to selectively recognize 2,4,6-trichlorophenol. The electrode modification material uses carbon quantum dot-polymer composite film, which is low in cost and environmentally friendly; the sensor is simple to prepare, the reaction conditions are mild, the process is easy to control, and it has good sensitivity to template molecules and strong anti-interference. Can be used for quantitative analysis of 2,4,6-trichlorophenol.
Owner:JIANGSU UNIV OF TECH
A preparation method of molecularly imprinted composite material for enrichment and separation of glycoproteins
ActiveCN105131178BMaintain natural structureHigh binding capacityOther chemical processesBoronic acidProtein blotting
The invention discloses a preparation method of a molecularly imprinted composite material for enriching and separating glycoproteins, belonging to the fields of material science and engineering and biological separation engineering. In this method, the surface of graphene oxide is firstly functionalized with boric acid, the template glycoprotein is fixed on the surface of graphene oxide through boron affinity, and the surface imprinting technology is used to form a protein imprinted layer on the surface of graphene oxide, and the template is eluted to obtain molecular imprinting. composite material. The molecularly imprinted composite material prepared by this method has a very good ability to specifically recognize the template glycoprotein, and also has the advantages of high binding capacity, strong anti-interference ability, fast binding and elution speed, and can be used for complex practical applications. The low-concentration glycoproteins in the sample are enriched, separated and detected.
Owner:沛县度创科技发展有限公司
A method for preparing molecularly imprinted sensors by photocatalytic raft polymerization and its application
ActiveCN108918483BAdvantages and Notable ImprovementsSignificant progressFluorescence/phosphorescenceMaterial electrochemical variablesFunctional monomerQuantitative determination
Owner:QINGDAO UNIV
Silk-screen printing electrode and method for synthesizing chloramphenicol molecular imprinting film
InactiveCN101387617BReduce productionLower requirementComponent separationMaterial analysis by electric/magnetic meansScreen printingInterference resistance
The invention relates to a silk screen printing electrode and a method for synthesizing chloramphenicol molecularly imprinted membrane, belonging to the macromolecule technical field. The invention embeds template molecules in the direct surface in-situ polymerization of a one-time silk screen printing electrode and elutes the template molecules, to form the cavities of fixed sizes and shapes andthe function groups of a fixed arrangement in the molecularly imprinted membrane, therefore, the molecularly imprinted membrane has shape memory property to the stereo structure of the template molecules, the chloramphenicol molecule of the object sample can be combined with the cavity specifically, to modify the electromechanical property of the molecularly imprinted membrane to improve the electric conductivity of the molecularly imprinted membrane and improve membrane passing current. The silk screen printing electrode coated with a chloramphenicol molecularly imprinted membrane can be connected with a current sensor to quickly and sensitively detect chloramphenicol density. The molecularly imprinted membrane can be used to detect chloramphenicol, having simple sample pretreatment, short detection time, strong interference resistance and batch production.
Owner:SHANGHAI JIAOTONG UNIV
Magnetic nanomaterials based on inorganic boronic acid modification and its preparation method and application
ActiveCN105536691BGood magnetic responseStrong specificity recognition abilityIon-exchange process apparatusOther chemical processesChemical compoundChemical separation
The invention provides a magnetic nano-material based on inorganic boric acid modification as well as a preparation method and application of the magnetic nano-material; the magnetic nano-material is obtained by modifying a layer of inorganic boric acid or salt compounds of the inorganic boric acid on the magnetic nano-material. The magnetic nano composite material is regular in shape and uniform in distribution, has excellent magnetic responsiveness, and specific recognition capability, can be used in many fields, such as the aspects of medicine purification systems, active ingredient extraction and separation, food processing, cosmetics, chemical separation and analysis, specifically applied to the complex component systems such as traditional Chinese medicinal materials, and has potential industrial process and application value.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
A gene nanoprobe for targeted lung cancer treatment and its preparation method and application
ActiveCN108753770BAct as a chemotherapeuticGood treatment effectOrganic active ingredientsAntineoplastic agentsAptamerTherapeutic effect
The invention relates to a gene nanoprobe for targeted lung cancer treatment as well as a preparation method and application thereof, and belongs to the technical field of biochemical nano materials.The gene nanoprobe for the targeted lung cancer treatment comprises an RNA nano hydrogel and DOX and / or TMPyP4 loaded on the RNA nano hydrogel; the RNA nano hydrogel comprises a DNA self-assembled single chain, a cyclization DNA template and a targeted aptamer. The gene nanoprobe has a strong specific recognition ability, precise targeting, small toxic and side effects, high killing and injuring intensity against lung cancer cells, good therapeutic effect, and great potential in the application of the lung cancer treatment.
Owner:LINYI UNIVERSITY
P53 protein monoclonal antibody and application thereof
PendingCN114292821AStrong specificity recognition effectStrong positive signalImmunoglobulins against animals/humansTissue cultureMonoclonalMolecular biology
The invention discloses a p53 protein monoclonal antibody and application thereof. The invention discloses an immunogen fragment of a p53 protein monoclonal antibody. The fragment is a p53 protein 1-80 region; a corresponding coating antigen peptide fragment screened by ELISA is a p53 protein 15-39 region, and one more cysteine is synthesized at the N end of the polypeptide. The cloning number of the monoclonal cell strain is 4H12E8, the subtype of a secreted antibody is IgG1, and the cell strain can secrete a p53 protein monoclonal antibody. Compared with an immunogen and a coating antigen, the p53 protein monoclonal antibody disclosed by the invention has the same peptide fragment, the prepared p53 protein monoclonal antibody has stronger specific recognition effect, can recognize p53 proteins in various samples, and has stronger positive signal and superior positive rate compared with a commercially common p53 protein monoclonal antibody; the method is suitable for detecting p53 protein in various tumor samples.
Owner:武汉赛维尔生物科技有限公司
Composite sensing electrode and preparation method and application thereof
PendingCN121899216AImprove conductivityImprove stabilityMaterial electrochemical variablesEngineeringMolecularly imprinted polymer
The invention discloses a composite sensing electrode and a preparation method and application thereof. The electrode comprises a basic electrode, an Al / Co-MOFs / rGO layer and a bifunctional monomer molecularly imprinted polymer film, wherein the Al / Co-MOFs / rGO layer and the bifunctional monomer molecularly imprinted polymer film cover the surface of the basic electrode. The preparation method comprises the following steps: preparing an Al / Co-MOFs / rGO suspension liquid, dripping the suspension liquid on the surface of an electrode, drying the electrode, placing the electrode in a solution containing o-phenylenediamine, dopamine and template molecules, carrying out an electropolymerization reaction, and demolding to obtain the electrode. The composite sensing electrode provided by the invention has the advantages of multiple binding sites, uniform dispersion, good conductivity, good structural stability, strong specific recognition capability and the like, can realize rapid and accurate detection of perfluorinated and polyfluoroalkyl substances, can effectively reduce false positive records existing in the detection process, and can be widely applied to detection of perfluorinated and polyfluoroalkyl substances. The method has the advantages of short detection period, high detection sensitivity, high accuracy and the like, and is high in use value and good in application prospect in the fields of environment monitoring, food safety, water quality condition diagnosis and the like.
Owner:HUNAN UNIV