85results about How to "Improve specific recognition ability" patented technology

The invention discloses molecularly imprinted polymers (MIPs) for detecting melamine and a preparation method thereof. The MIPs are prepared by the steps as follows: cyromazine is used as a virtual moulding board and is polymerized with a functional monomer, a cross linker agent, a pore-foaming agent and an initiator at the temperature from 50 to 80 DEG C to obtain a bulk polymer; and after being processed by the operations of pulverization, screen separation, moulding board removal by acetic acid methanol and methanol, the bulk polymer is processed by vacuum drying to prepare the MIPs which have high cross reaction and outstanding selectivity to melamine. The invention selects the cyromazine as the virtual moulding board to prepare the MIPs, and avoids the problem that the melamine is hard to dissolve in a polymerization system and the problem that when the MIPs prepared by using the melamine as the moulding board is used for solid phase extraction of fill materials, moulding board leakage can lead to an inaccurate result; the invention selects the mixed system of methanol and water as the pore-foaming agent, and the prepared MIPs have high compatibility with the melamine in an aqueous solution; and used as a sample pre-processing material for analyzing the melamine, the prepared MIPs have broad application prospect.

The invention discloses a double recognition dopamine imprinting electrochemical sensor and a preparation method and application thereof. The method comprises the following steps: (1) conducting contact reaction on 3-amino boronic acid, 2,5-dimethoxy furan and acetic acid to prepare functional monomers; (2) in the presence of a buffer solution, mixing the functional monomers and dopamine to form a polymerization liquid, placing a glassy carbon electrode in the polymerization liquid, and conducting scanning preparation of a polymer film modified electrode in the polymerization liquid in accordance with the a cyclic voltammetry; and (3) placing the polymer film modified electrode in an acidic solution for electrochemical elution, so as to prepare the double recognition dopamine imprinting electrochemical sensor. The electrochemical sensor provided by the invention has excellent specificity recognition ability and anti-interference ability for the detection of dopamine.

The invention discloses a cell probe complex for detecting human breast cancer cells MCF (Michigan cancer foundation)-7 based on Raman spectrum and a method for preparing the cell probe complex. The cell probe complex is characterized in that nucleic acid aptamer capable of realizing a targeting effect with the MCF-7 cells is used as a template, bimetal alloy nano particles of gold and silver are deposited on a basic group of a DNA (deoxyribose nucleic acid) chain of the nucleic acid aptamer by a photocatalysis method, and Raman signal molecules rhodamine 6G (Rh6G) is fixedly loaded on the surface of each nano particle. The structure of the probe complex is chain-shaped as shown by a photo of a transmission electron microscope, and the probe complex is capable of realizing the targeting effect with the MCF-7 cells. Characteristic absorption peaks of the rhodamine 6G appear on the Raman spectrum under the effect of the MCF-7 cells and the probe complex, and the human breast cancer MCF-7 cells can be effectively, sensitively and specifically detected.

The invention discloses a rapid detecting sensor for microcystins. The rapid detecting sensor utilizes a photochemistry polymerization method or an electrochemical polymerization method to synthesize a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is reduced, and the specific adsorption capacity is strengthened; meanwhile, a Lamb wave piezoelectric membrane is utilized as an effector of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, the cost is reduced, the performance of the sensor is strengthened, the trace detection of the microcystins is realized, and thus the rapid detecting method for the microcystins, with high sensitivity, rapidness, low cost and low operation requirements, is established.

The invention discloses a layered fluorine-containing metal-organic framework material for adsorbing and separating acetylene ethylene, the structural general formula is ML2A, M is a metal ion, L is abroken line type organic ligand, A is an inorganic fluorine-containing anion, and the metal ion M is at least one of Cu<2+>, Fe<2+>, Co<2+>, Ni<2+> and Zn<2+>, wherein the broken line type organic ligand L is at least one of 4, 4'-dipyridylsulfoxide and 4, 4'-dipyridylsulfone, and the inorganic fluorine-containing anion A is at least one of SiF6<2->, GeF6<2->, ZrF6<2->, SnF6<2->, TiF6<2->, NbOF52<->, WO2F4<2-> and MoO2F4<2->. The layered fluorine-containing metal-organic framework material can be prepared by adopting an interface diffusion method, a solvothermal method or a room-temperature stirring method. The layered fluorine-containing metal-organic framework material is used as an adsorbent and is in contact adsorption with a gas mixture containing acetylene and ethylene, and efficient separation of acetylene/ethylene can be realized at normal temperature, low acetylene content and low acetylene partial pressure.

The invention discloses a method for detecting pathogenic bacteria in foods by isothermal rapid amplification and belongs to the technical field of food safety detection. The method comprises the following steps: determining detection target nucleic acid aiming at each bacterium through multiple sequences; designing a specific molecular beacon probe and a primer; taking a genome DNA (Deoxyribonucleic Acid) of each strain as a template and establishing real-time fluorescence CSDPR; carrying out the isothermal nucleic acid amplification and identifying the specificity, sensitivity and minimum limit of detection of a CSDPR method. The detection of an actual sample determines that the method established by the invention has practical feasibility. The method disclosed by the invention has commonality on detection of various pathogenic bacteria in the foods and the pathogenic bacteria comprise staphylococcus aureus, salmonella, enterohemorrhagic escherichia coli, shigella and the like; only if a specific part in the molecular beacon probe needs to be replaced, effective detection of the different pathogenic bacteria can be realized; and the method has high detection sensitivity and high specificity, simple principle and operation and the pretreatment of the sample is simple.

The invention, which belongs to the field of environmental monitoring and electrochemical sensors, relates to a tetrabromobisphenol A molecularly-imprinted electrochemical sensor, and a preparation method and application thereof. A glassy carbon electrode modifying a multi-walled carbon nanotube and gold nanoparticles is used as a working electrode; a polymer film of polypyrrole and tetrabromobisphenol A is prepared by using a cyclic voltammetry method; and then tetrabromobisphenol A is eluted to obtain a molecularly-imprinted electrochemical sensor for direct electrochemical detection of tetrabromobisphenol A. Compared with the traditional detection method, the provided method has characteristics of convenient operation, high sensitivity, high specific recognition ability, high reproducibility and strong anti-interference ability.

The invention discloses a method for preparing a magnetic halloysite molecularly imprinted polymer with specific adsorption to 2,4-dichlorophenoxyacetic acid. The preparation method comprises the following steps: preparing magnetic halloysite nano-tubes by using corroded halloysite nano-tubes by a coprecipitation method; reacting the magnetic halloysite nano-tubes and methacrylate in methylbenzene to obtain magnetic halloysite nano-tubes with double-bond-modified surfaces; after polymerizing the magnetic halloysite nano-tubes with double-bond-modified surfaces, the polymerized monomers and a crosslinker by taking the 2,4-dichlorophenoxyacetic acid as a template, then removing the 2,4-dichlorophenoxyacetic acid to obtain the magnetic halloysite molecularly imprinted polymer with specific adsorption to the 2,4-dichlorophenoxyacetic acid. The raw materials used in the preparation method are low in cost; the preparation method is easy to operate and easy to implement; the prepared magnetic halloysite molecularly imprinted polymer is stable in structure, has specific adsorption to the 2,4-dichlorophenoxyacetic acid, and can effectively separate and remove the 2,4-dichlorophenoxyacetic acid from a complex system.

The invention discloses a piezoelectric detection sensor for microcystic toxins based on a molecular imprinting technology. The piezoelectric detection sensor is characterized in that a photochemistry polymerization method or an electrochemical polymerization method is utilized to compound a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is shortened, and the specific adsorption capacity is strengthened; meanwhile, a quartz crystal serves as an effective apparatus of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, and the cost is reduced. The piezoelectric detection sensor provided by the invention has the advantages that the property of the sensor is improved, the trace detection of the microcystic toxins is realized, and the microcystic toxins detection method which is high in sensitivity, high in speed, low in cost, and low in operation requirement is established.

The invention relates to a multi-hydrogen-bond melamine core-shell molecularly imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: by adopting SiO2 as an inner core, performing modification on the surfaces of SiO2 nano particles by using 3-aminopropyltriethoxysilane and glutaraldehyde, and connecting a functional monomer 6-aminouracil; and performing polymerization reaction on melamine as a template molecule, methacrylic acid, an initiating agent and a crosslinking agent to prepare the multi-hydrogen-bond melamine core-shell molecularly imprinted polymer. The multi-hydrogen-bond melamine core-shell molecularly imprinted polymer disclosed by the invention can be used for exchanging the roles of a functional monomer in a conventional three-hydrogen-bond molecularly imprinted system and the template molecule, adopts the functional monomer in the conventional system as the template molecule, adopts a template molecule in the conventional system as the functional monomer in a system disclosed by the invention, has very high selectivity, has specific binding only aiming at melamine and triazine compounds, and has good selective enrichment and adsorption effects in highly-specific food detection.

The invention belongs to the technical field of molecularly imprinted polymers, and particularly relates to a preparation method of protein affinity blotting aquagel polymers based on acrylamide groupmetal chelation monomers. The invention aims at providing the preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers by aiming atthe technical analysis and the existing problems. The preparation method of the protein affinity blotting aquagel polymers based on the acrylamide group metal chelation monomers has the technical scheme that according to the method, after the acrylamide group monomers are chelated with divalent metal ions, target protein is subjected to specific adsorption combination and removal; then, polymers are obtained. The preparation method has the following characteristics that the acrylamide group monomers have the metal chelation capability; the molecular mark aquagel has the temperature and pH duplex sensitivity; the reverse phase suspension technology is used; the polymers are aquagel polymers.

The invention provides a preparation method and application of a molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually. According to the preparationmethod, two types of quantum dots are used as fluorescence signal sources, functional monomers and hydrophilic monomers are added simultaneously, HAV and HBV are used as templates to be imprinted on different carriers respectively, obtained imprinted polymers are mixed after the templates are eluted, and the fluorescence sensor capable of detecting the two types of viruses simultaneously is obtained. Through the sensor, the advantages of the hydrophilic monomers N-isopropyl acrylamide (NIPAAm) and the advantages of the functional monomers zinc acrylate are combined simultaneously, and high-specificity recognition of the two types of viruses HAV and HBV is realized. Since the quantum dots have different fluorescence quenching degrees after being combined with target objects with different concentrations, visual detection is realized. The provided strategy lays a good foundation for simultaneously detecting multiple similar viruses through virus molecule imprinting and meanwhile has a potential ability in clinical treatment and diagnosis of viral diseases.

The invention relates to a preparation method of a fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles. The fluorescent nanoparticles are taken as a carrier, and linear nonapeptide and adriamycin of an extracellular region where HER2 is over expressed are used as double templates to synthesize the imprinted polymer. The method comprises the followingsteps: preparing fluorescent silicon nanoparticles (Si NPs), carrying out silanization on the Si NPs; and preparing the fluorescent double-template antigenic-determinant imprinted polymer based on thesilicon nanoparticles. According to the method, the imprinted polymer is synthesized by combining antigenic-determinant imprinting, double-template imprinting and a surface imprinting method, the operation is simple and convenient, the process saves time, and the material has a targeting imaging function and a targeted therapy function. Zinc acrylate and acrylamide are adopted as functional monomers, and the templates are jointly fixed through metal chelating and action of hydrogen bonds, so that the specific recognition effect of the material on a target object can be effectively improved, and controlled release of medicines can be realized by utilizing an acidic pH value. The synthesized fluorescent imprinted polymer efficiently combines high sensitivity of fluorescence and high selectivity of a molecular imprinting technology.

The invention discloses an indicator diagram fault diagnosis method based on a generative adversarial neural network, and belongs to the technical field of oil extraction fault diagnosis, and the method comprises the following steps: carrying out the data cleaning of indicator diagram sample library data; based on an oil production engineering theory and typical indicator diagram characteristics, performing feature extraction on indicator diagram data points; adopting a generative adversarial neural network to generate a small number of fault category samples, and performing conditional constraint on the output of a generator network in the generation process; dividing the data into a training set, a verification set and a test set based on the original sample and the generated sample; an Xgboost classification algorithm is adopted to classify the samples; comprehensively evaluating a fault diagnosis result by utilizing the accuracy rate and the recall rate; and performing real-time monitoring and diagnosis on the fault by using the trained classification model, and judging the fault type in real time. The method can significantly improve the specific recognition capability of the classification model for the fault sample, and reduces the false alarm/missing report rate of the fault.

The invention discloses a detection method for malachite green in aquatic products. The detection method comprises experiment steps of preparing a magnetic molecularly imprinted polymer, establishinga method based on an aptamer and ferroferric oxide mimic enzyme catalysis and the like. According to the detection method, firstly, a dopamine polymerization method is used for preparing a malachite green (MG) magnetic molecularly imprinted polymer (MG-MMIPs); trace MG in a sample is adsorbed by utilizing the MG-MMIPs, so that the aims of enriching a target object and removing a complicated mediumare realized; then after enriched MG is desorbed, a detection method of the aptamer and ferroferric oxide mimic enzyme catalysis is used for detecting. The method has the sensitivity of 5 nmol L<-1>and the lowest detection limit reaches 3.6 nmol L<-1>. A specific experiment aiming at two structural analogues (including brilliant green and crystal violet) of MG and other bacteriostatic drugs (including sulfaguanidine, sulphanilamide, oxytetracycline hydrochloride and chloramphenicol) shows that the method has good selectivity in MG detection.

The invention belongs to the field of immunological detection, and relates to a kit, in particular to a kit for rapidly detecting peripheral T cell lymphoma and a use method of the kit. The kit comprises a reagent I and a reagent II, the reagent I comprises two groups of monoclonal antibody mixtures, and the first group of monoclonal antibody mixture comprises TRBC1, CD2, CD3, CD5, CD7, CD16, CD56, CD57 and CD45; the second group of monoclonal antibody mixture comprises the following components: TRBC1, TCR-gamma delta, CD3, CD8, CD4, CD7 and CD45; and the reagent II is hemolysin. The kit provided by the invention realizes the rapid and accurate detection of the peripheral T cell lymphoma (PTCL), is simple and convenient, greatly reduces the requirement on the sample size, and reduces the burden of a patient.