89results about How to "Enrichment" patented technology

The invention provides an enrichment and analysis method for circulating tumor cells. The method mainly comprises the following steps: subjecting a biological fluid sample to red blood cell lysis treatment; adding immune beads specifically bonding with white cells, red cells and/or platelets for incubation; and filtering cell suspension by using a filter membrane and enriching circulating tumor cells. According to the invention, through integration and optimization of CTCs enrichment methods such as red blood cell lysis treatment, an immune bead method and membrane filtration, the enrichment effect of the circulating tumor cells is greatly improved; with the method, the removal rate of leukocytes is greater than 99.9%, and the enrichment rate of tumor cells is greater than 80%. Meanwhile, the invention also provides a kit using the enrichment method for the circulating tumor cells and discloses a device for enrichment of the circulating tumor cells. The invention also provides an analysis method for the enriched circulating tumor cells. With the analysis method, analysis of dynamic changes like the amount of tumor cells, gene mutation and gene expression profiles can be realized.

The invention relates to a singlet state oxygen generation method, and belongs to the technical field of chemical oxygen-iodine lasers. The method comprises the following steps: mixing an alkaline ionic liquid insoluble in water with an aqueous hydrogen peroxide solution to form a two phase transfer system, and reacting the transferred ionic liquid phase with chlorine to generate singlet state oxygen in order to realize water and singlet state oxygen isolation. The method is helpful for reducing the quenching effect of water on singlet state oxygen, and reduces the content of water in the singlet state oxygen, and the alkaline ionic liquid has an enrichment effect on hydrogen peroxide to make effective solving of the bottleneck problem of hydrogen peroxide concentration restriction in the raw material regeneration process of chemical oxygen-iodine lasers under traditional technological conditions be possible, so the method is of great significance to improve the singlet state oxygen yield of the chemical oxygen-iodine lasers, reduce the water content of the singlet state oxygen and solve the raw material regeneration problem.

The invention provides a surface enhanced Raman scattering substrate, which comprises a polylactic acid electrostatic spin film and nano metal particles on the surface or in the inner of the polylactic acid electrostatic spin film, wherein the nano metal particles carry positive charges. The surface enhanced Raman scattering substrate has the advantages of high enhancing ability, good repeatability, long storage period, and capability of enriching articles to be detected. The invention also provides a preparation method of the surface enhanced Raman scattering substrate. The preparation method comprises the following steps: (1) dissolving polylactic acid into an organic solvent to obtain a spinning liquid, and using the spinning liquid to carry out electrostatic spinning to obtain a polylactic acid electrostatic spin film; (2) arranging nano metal particles on the surface and in the inner of the obtained polylactic acid electrostatic spin film through a spotting mode so as to obtain the surface enhanced Raman scattering substrate. The preparation method is simple and is suitable for massive production. The prepared surface enhanced Raman scattering substrate can be applied to food analysis, environment analysis, etc.

The invention relates to a preparation method of an adsorption-photocatalysis compound type formaldehyde removing coating, relating to a preparation method of a coating and aiming at solving the problems of low formaldehyde degradation efficiency and incapability of enriching formaldehyde in an existing photocatalysis coating. The preparation method disclosed by the invention comprises the following steps: (1), preparing ACF (Active Carbon Fiber) modified by concentrated nitric acid; (2), mixing butyl titanate, ethanol and acetic acid; (3), mixing ethanol, acetic acid and water, adding urea and iron nitrate nonahydrate crystals, and adjusting pH; (4), preparing TiO2 sol containing iron and nitrogen; (5), preparing ACF loaded with TiO2; (6), preparing TiO2/ACF compound powder; and (7), mixing the TiO2/ACF compound powder with the coating. By means of the enrichment action of ACF, the problem of low TiO2 efficiency is solved to a great extent; by means of the catalytic action of TiO2, the saturated regeneration problem of an adsorbent is effectively solved; the characteristics of the ACF and the TiO2 are complementary; the formaldehyde degradation efficiency is greatly improved. The preparation method disclosed by the invention can be used for preparing the adsorption-photocatalysis compound type formaldehyde removing coating.

The invention discloses a tuckahoe extract which is prepared through ethanol extraction and macroporous absorption resin purification. The tuckahoe extract enriches more effective parts. Moreover, a rat memory disorder model test proves that the tuckahoe extract can improve the learning and memory capacity of a model rat, so that the number of errors of the model rat is reduced and the latent period is prolonged.

The invention discloses a method for production of sulfuric acid by flue gas desulfurization and for sulfuric acid purification and a device thereof. The method comprises the following contents: (1) flue gas undergoes heat exchange and then enters a dedusting quench tower for primary dedusting and cooling process; (2) the flue gas enters a desulfurization tower and successively passes through a deep dedusting unit, a desulfurization unit and a washing unit from the bottom to the top; (3) the obtained overflow rich in an absorption liquid successively passes through a desulfurization unit and a deep dedusting unit and then enters a dedusting quench tower for solid-liquid separation, and the residual gas phase is discharged into the atmosphere; and (4) the absorption liquid separated from solid waste enters an absorption liquid regeneration tower, and enters a sulfuric acid storage tank in the form of dilute sulfuric acid after transition metal cations in the absorption liquid are removed through H<+> in resin. By the method and the device, SO2 in the flue gas can be directly converted into sulfuric acid through oxidation, and a sulfuric acid solution with high concentration is prepared by concentration. The purified flue gas can reach the national or local relevant standards. The method and the device are suitable for recovery processing of flue gas containing various concentrations of sulfur dioxide.

The invention discloses a method for repairing sludge heavy metal by combination of DTPA and ryegrass. According to the method, DTPA of different concentrations serving as chelating agents is combined with the ryegrass and a corn interlayer to improve enrichment of heavy metal on the upper portion of a ryegrass land and to restrain the ability of the sludge heavy metal to move to deep soil in a leaching mode. The corn interlayer comprises 40-60g of vermiculite, 40-80g of zeolite and 40-100g of corncobs. According to the method, sludge and soil mixed substrate serves as culture substrate of the ryegrass, when turfgrass grows to a certain stage, DTPA is used, rainfall and pH of TianJin are simulated for leaching, and the content of the heavy metal in the upper portion of the ryegrass land and in leaching solutions is tested. As is shown in an experiment result, a combination of 10mmol.kg-1 DTPA and the interlayer comprising the corncobs has the best repairing effect on the heavy metal in the sludge, repairing cost is low and pollution to underground water is least.

The invention relates to an improving agent for repairing contaminated soil and a modifying method. The improving agent is obtained through mixing of arbitrary one or more of 1-3 parts of calcium magnesium phosphate fertilizer, 0.5-7 parts of lime and 1.5-12 parts of modified zeolite. The improving agent is mixed with soil, passivation is performed, and planting of Bermuda grass on the soil and other improving methods are adopted to achieve the purpose of contaminated soil repairing. A chemical technology is combined with a plant technology, not only can the soil structure be protected so as to the antimony-contaminated soil in the case of low cost, but also the environment can be beautified.

The invention discloses a method for detecting quinolone antibiotics by an electrochemiluminescence sensor. According to the method, nanogold and Ru(bpy)[3]<2+> are fixed on the surface of a glassy carbon electrode through a Nafion membrane, a Ru(bpy)[3]<2+> complex has an oxidation reaction for oxidizing divalent Ru into trivalent Ru, the generated trivalent Ru is reduced into the divalent Ru by the quinolone antibiotics, and fluorescent light is emitted in the reduction reaction process; the Nafion membrane can generate a static attraction force to ions and especially the hydrophobic organic positive ions can be always preferentially bonded with the Nafion membrane to be fixed in the membrane; the nanogold improves the electrochemical oxidation site of Ru(bpy)[3]<2+>; the Nafion membrane also has an enrichment effect on the quinolone antibiotics in a detected sample, so that the detection sensitivity is greatly improved; the logarithm value of the change of the electrochemiluminescence intensity of a system and the logarithm value of the concentration of the quinolone antibiotics in the system before and after the addition of the quinolone antibiotics form a good linear relationship; the content of the quinolone antibiotics in the detection system can be determined through a standard curve.

The invention discloses a preparation method and application of a Pd-Ru catalyst with a mesoporous structure. The preparation method is characterized by including the steps of firstly, allowing hydroquinone to have reaction with allyl halohydrocarbon to obtain p-triallyloxy benzene; secondly, allowing the p-triallyloxy benzene, divinyl benzene and 4-vinyl benzoic acid to polymerize in a mixed solvent of ethers and water to obtain TriAllB-DVB-VBA; in an ether solvent, mixing the TriAllB-DVB-VBA with Pd(OAc)2 and RuCl3.6H2O, stirring at room temperature, and adding KBH4 for reduction to obtain the catalyst TriAllB-DVB-VBA@Pd-Ru. The catalyst prepared by the preparation method has good catalytic activity and reusability in a preparation method using the catalytic hydrogenation of 6-benzyl-5, 7-dioxo-pyrrolo [3, 4, b] pyridine to prepare 8-benzyl-7, 9-dioxo-2, 8-diazabicyclo [4, 3, 0]-1-alkenyl nonane.

The invention discloses an integrated circulating-storing-collecting-distributing energy pile, which mainly comprises energy blocks, primary circulating cabinets, energy pile boxes, etc. The energy blocks are integrated and stored in the primary circulating cabinets in a charging station; the primary circulating cabinets are integrated to the energy pile boxes, changed in the charging station and then transported to a power exchanging station to exchange the primary circulating cabinets or the energy pile boxes with the power exchanging station; the exchanged energy pile boxes are transported back to the charging station to add energies; therefore, the energy pile boxes are circulated. The energy blocks loaded by the primary circulating cabinets of the power exchanging station are mutually exchanged with the energy blocks in the required vehicles of users; and the primary circulating cabinets after mutually exchanging the energy blocks are circulated. The integrated circulating-storing-collecting-distributing energy pile can be adopted to exchange energies for the required vehicles in 2-3 minutes under the premise of not damaging the service life, and can enrich the separated or waste energies in an energy adding station. The integrated circulating-storing-collecting-distributing energy pile is used for quickly replacing power energies for the required vehicles such as electric automobiles, electric bicycles, etc.

The invention discloses a cell membrane chromatographic column for identifying a microscale target component in a complex sample and a preparation method thereof, and an identification method of the target component. The cell membrane chromatographic column (identification column for short) for identifying the microscale target component in the complex sample is filled with a cell membrane chromatographic stationary phase for expression of specific receptors. Screening specificity of the target component in the complex sample can be enhanced, and it is of great significance for enhancing screening efficiency and expounding mechanism of action. According to the identification method, by specific identification of the target component in the complex sample through the identification column and by online enrichment of the microscale target component through an enriching column, separation and identification are further carried out by high performance liquid chromatography-mass spectrometry. The method of the invention has the characteristics of efficient identification and online enrichment of the microscale target component in the complex sample, can help enhance analysis efficiency, raise sensitivity for analysis and realize high-efficiency screening of the target component in the complex sample.

The invention discloses a detection method for malachite green in aquatic products. The detection method comprises experiment steps of preparing a magnetic molecularly imprinted polymer, establishinga method based on an aptamer and ferroferric oxide mimic enzyme catalysis and the like. According to the detection method, firstly, a dopamine polymerization method is used for preparing a malachite green (MG) magnetic molecularly imprinted polymer (MG-MMIPs); trace MG in a sample is adsorbed by utilizing the MG-MMIPs, so that the aims of enriching a target object and removing a complicated mediumare realized; then after enriched MG is desorbed, a detection method of the aptamer and ferroferric oxide mimic enzyme catalysis is used for detecting. The method has the sensitivity of 5 nmol L<-1>and the lowest detection limit reaches 3.6 nmol L<-1>. A specific experiment aiming at two structural analogues (including brilliant green and crystal violet) of MG and other bacteriostatic drugs (including sulfaguanidine, sulphanilamide, oxytetracycline hydrochloride and chloramphenicol) shows that the method has good selectivity in MG detection.

The invention belongs to the field of sewage treatment, and particularly relates to a double-layer carbon source microsphere with a function for advanced treatment of low C/N ratio domestic sewage andpreparation and application of the double-layer carbon source microsphere, in particular to a double-layer microsphere built by gelatin and sodium alginate. A macromolecule carbon source is embeddedwith an inner layer of the microsphere, and a microsphere carbon source is embedded with an outer layer of the microsphere. When the low C/N ratio domestic sewage is deeply treated, a certain amount of the microspheres are directly put into the domestic sewage. The preparation method of the double-layer carbon source microsphere is simple, reaction conditions are mild, and used carrier materials are degradable materials. The microspheres are directly put into the domestic sewage, the carbon sources are released, the appropriate carbon sources can be provided to enable biological denitrification to be carried out normally, pollutants cannot be introduced, the carrier materials can naturally degrade after treatment, and secondary pollution cannot be caused to the environment.

Video streams, control programs, and an index table are recorded in a recording medium. The video streams include a progressive-picture video stream and an interlaced-picture video stream, and the frame rates for the video stream constructed from progressive pictures include a mandatory rate and an optional rate. The index table indicates the association between a plurality of titles and the control programs. A control program associated with a first play title and top menu title from among the titles excludes, from a reproduction target, a reproduction route with which pictures are progressive pictures and the frame rate is the optional frame rate, and instructs reproduction by way of a different reproduction route.

The invention relates to the field of heat stabilizers for PVC, in particular to a special low-odor phenol-free barium-zinc stabilizer for a toy film and a preparation method of the barium-zinc stabilizer. The stabilizer is prepared from components in parts by weight as follows: 100 parts of a main stabilizer, 10-15 parts of a porous thiophene polymer, 5-8 parts of a lubricant, 4-9 parts of a phenol-free antioxidant and 20-30 parts of an auxiliary stabilizer. The porous thiophene polymer is added to the formula and has higher BET value and high specific surface area, thereby having an enriching function and high adsorption capacity, heat stability during PVC processing is improved remarkably, and the probability of discoloration is low; by means of the rigid structure of the polymer, mechanical properties and weather fastness of a product can be improved; the preparation method of the heat stabilizer is simple, convenient to operate and environmentally friendly, and the prepared heat stabilizer has good heat stability and processability.