Two-dimensional capillary electrophoresis appareatus and use thereof
A technology of capillary electrophoresis and capillary, which is applied in the preparation method of peptides, organic chemistry, peptides, etc., can solve the problems that have not been constructed with 2D-CE, the removal effect is not complete, and the detection sensitivity of samples is reduced, so as to achieve high peak capacity, The effect of reducing the requirement for replacement capacity and high separation capacity
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Embodiment 1
[0041] Such as figure 1 As shown, the first dimension adopts the M-IPG CIEF method, the positive buffer is 20mmol / L glutamic acid, and the negative buffer is 20mmol / L NaOH. The sample is filled with the entire M-IPG column, the overall column length is 20 cm (the lengths used in the present invention are 15, 20, 30 and 40 cm), and the inner diameter is 100 μm (can be 100, 200 and 250 μm). The second dimension adopts CZE to separate, and separation buffer is 20mmol / L glutamic acid, and capillary total length is 30cm (overlength adopted among the present invention is 20 and 30cm), and effective length is 20cm (overlength adopted among the present invention is 10 and 30cm). 20cm), the inner diameter is 50μm (can be 75μm).
[0042] The sample is a mixture of 7 standard proteins: trypsin inhibitor (trypsin inhibitor, pI 4.5), bovine serum albumin (BSA, pI 4.8), albumin (albumin, pI 4.9), β-lactoglobulin (β -lactoglobulin, pi 5.0), hemoglobin (Hemoglobin, pi 7.1), urease (urease, ...
Embodiment 2
[0047] The difference from Example 1 is that the sample used is protein extracted from milk, and the experimental operation is the same as that of Example 1.
[0048] Such as Figure 5 As shown, the separation of milk protein by one-dimensional M-IPG-CIEF is very poor, and the protein is not separated. Such as Figure 6 As shown, the separation results of proteins in milk have been significantly improved by using 2D-M-IPG-CIEF-CZE. Depend on Figure 7 It can be seen that the milk protein is better separated.
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