17 results about "Ph gradient" patented technology

The invention discloses an acid mine pollution treatment method based on microbial regulation and control, and belongs to the technical field of environmental protection engineering, and the acid mine pollution treatment method comprises the following steps: S1, screening and domesticating acid-resistant and heavy metal-resistant functional microbial communities; s2, constructing a sectional bioreactor, and filling the sectional bioreactor with a corresponding composite biological carrier; s3, inoculating the functional microbial community obtained by domestication in the step S1 into the sectional reactor constructed in the step S2, and carrying out biofilm culturing in simulated AMD; and S4, introducing AMD to be treated into the reaction system in the step S3, and regulating and controlling microbial activity through pH gradient regulation and dynamic supply of electron donors to realize heavy metal precipitation and water quality remediation. According to the method, closed-loop treatment of sulfate reduction, heavy metal precipitation and acid neutralization is achieved through the synergistic effect of microbial regulation and control and the functionalized biological carrier, and the problems of efficiency, cost and secondary pollution of traditional AMD treatment are solved.

The invention discloses an iron-mud-based environmental functional material based on an electrochemical-ferrate synergistic effect as well as a preparation method and application of the iron-mud-based environmental functional material. The material is derived from an iron sludge byproduct generated in the process of cooperatively treating wastewater by electrochemical oxidation and ferrate. Due to the influence of active oxygen species, electric field action and local pH gradient in an electrochemical system, the forming process of the iron mud is different from that of conventional chemical precipitation, the obtained material has an amorphous and high-specific-surface-area unique structure and is endowed with remarkable oxidation capacity, and cooperation of oxidation and adsorption functions is achieved. The material disclosed by the invention can be used for efficiently removing anion pollutants such as arsenic, antimony and phosphate in a water body. According to the invention, solid wastes generated by a combined process are converted into a high-performance adsorption material, so that the resource utilization of treating wastes with wastes is realized, the comprehensive operation cost of the advanced oxidation process is effectively reduced, and the method has good environmental and economic benefits and application prospects.

The application discloses a kind of on-line double-gradient pre-concentration detection methods of soil heavy metal ions based on microchip electrophoresis non-contact conductivity detection, including preparation of the analyte sample to be measured, preparation of high concentration buffer as background buffer, preparation of microchip, microchip electrophoresis process and the like steps.When testing, the background buffer contains 2-(N-morpholinyl)ethanesulfonic acid, L-histidine and electroosmotic flow inhibitor;The analyte sample to be measured contains sample matrix liquid and sample extraction stock solution.Among them, the sample matrix liquid is low-concentration buffer, and glacial acetic acid is used to reduce pH value.In testing, due to the existence of concentration gradient and pH gradient between the analyte sample solution to be measured and the background buffer, the ions to be measured in the sample will slow down and accumulate at the contact surface of the two during migration, realizing on-line pre-concentration, and improving the detection sensitivity of the ions to be measured.The application greatly improves the separation degree of multiple heavy metal ions, and effectively improves the detection sensitivity of heavy metal ions.

The invention relates to the technical field of biological catalytic synthesis, and discloses a medical magnesium L-threonate synthesis method, which adopts three layers of magnetic carriers to immobilize L-threose oxidase, and realizes pH gradient regulation and control through a two-stage series reactor. The pH value of the first-stage reactor is 7.0 + / -0.2, enzyme catalytic oxidation is carried out, the pH value of the second-stage reactor is 8.0 + / -0.2, a magnesium complex reaction is carried out, and finally the carrier is recovered through magnetic separation. According to the method, the conversion rate is increased from 70-80% to 88 + / -2%, the product purity is increased from 90-95% to 99.0 + / -0.3%, the carrier can be recycled 6-10 times, the enzyme activity retention rate is larger than or equal to 75% when the carrier is used for the sixth time, and efficient and green synthesis of medical grade magnesium L-threonate is achieved.

The invention discloses a pH value real-time regulation and stabilization method and system for online column separation, and belongs to the technical field of ion exchange chromatographic separation.The method comprises the steps that a proton disturbance signal is applied to a chromatographic column, an outflow response signal of the chromatographic column is detected, and a parameter map representing the dynamic buffer capacity characteristic of the chromatographic column is obtained; the parameter atlas and the target pH gradient are input into a physical transmission model based on convection, diffusion and adsorption coupling equations, and the physical transmission model outputs a predistortion pH gradient program used for compensating dynamic buffer capacity characteristics; according to a fluid proportion requirement which is defined by a pre-distortion pH gradient program and changes along with time; according to the method, dynamic buffer capacity characteristics can be obtained through a proton disturbance test, a pre-distortion pH gradient program is constructed, and closed-loop iterative optimization is combined, so that interference of the dynamic buffer capacity of the chromatographic column on proton transfer is effectively counteracted, a post-column effluent pH curve is accurately matched with a target linear gradient, and the problems of pH nonlinear distortion and a platform area are eliminated.

The invention relates to the technical field of wastewater treatment, in particular to a phosphorus-containing wastewater treatment system and method.The phosphorus-containing wastewater treatment system comprises a fluidized bed reactor, a separation net, a pH sensor and a pH controller, and a seed crystal bed layer for inducing struvite crystallization is arranged in the fluidized bed reactor; the fluidized bed reactor is divided into a first subarea and a second subarea which are mutually communicated by the separation net, and the surface coating prevents Hor OHdiffusion; the subarea adding device is used for respectively adding a pH regulator into the two areas; the pH sensor measures the pH in real time, and the pH controller adjusts the dosage according to a set target to form a controllable pH gradient. According to the phosphorus-containing wastewater treatment system and method, the long-term stability of the pH gradient is ensured, nucleation and growth space separation is achieved, generation of fine grains is greatly reduced from the source, and the quality of phosphorus products is improved.

Herein is reported a method for separating full AAV particles from empty AAV particles, the method comprising the steps of applying a solution comprising full, empty and partially filled AAV particles to an AAV affinity column comprising an AAV affinity material and thereby binding the AAV particles to the AAV affinity material, wherein the solution has a pH in the range of 7.4 to pH 4,5 eluting full AAV particles from the AAV affinity column by applying a pH gradient from the pH of the solution of the first step to a pH in the range of 4 to 2.5, wherein the pH of the applying step and the pH of the eluting step differ in a least 0.5 pH units, and recovering full AAV particles from the eluate, and thereby separating full AAV particles from empty and partially filled AAV particles.

The invention discloses a preparation method of boehmite hydrated alumina nanocrystals based on pH gradient regulation and control, and relates to the field of preparation of inorganic non-metallic materials. Aiming at the problems of non-uniform particle size, high cost and difficulty in industrialization of boehmite caused by single pH regulation and control in the prior art, the method comprises the steps of precursor pretreatment, three-stage pH gradient hydrothermal reaction and post-treatment, wherein a nonionic stabilizer is added in the pretreatment stage; the hydrothermal reaction is regulated and controlled in three stages of crystal nucleus induction, crystal growth and morphology stabilization; the post-treatment is matched with a dispersing and impurity removing process. The method does not need expensive raw materials, the prepared product is uniform in particle size, the purity is larger than or equal to 99.90%, the method is adaptive to conventional equipment, industrial mass production can be achieved, defects in the prior art are overcome, and the method is suitable for the fields of lithium battery diaphragm coatings and the like.

The invention relates to the technical field of information, and particularly provides a method for monitoring the influence of pH gradient on energy conversion, which comprises the following steps: by taking electronegative nanofiber as an ion transport framework material, forming an interface hydrogen bond and electrostatic crosslinking with an electronegative natural polymer doped component to prepare a composite membrane with a nano-channel structure; at least two groups of pH difference value pairing combinations with different pH difference values are set, electrolyte solutions with concentration difference on the two sides of the composite membrane are prepared, and the pH difference value pairing combinations are asymmetric pairing; placing the composite membrane on two sides of the electrolyte solution with the pH difference and the concentration difference to realize ion directional transmission and generate transmembrane potential to perform permeation energy conversion; monitoring data are calibrated and screened, and the quantitative incidence relation of the pH difference value, the electrolyte concentration difference and the energy conversion efficiency is established.

Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and / or elution of fractions containing analytes.

This invention relates to the field of monitoring corrosion morphology and pH dynamic distribution in steel, and particularly to a rapid and intelligent monitoring method and system for in-situ monitoring of pitting morphology and surrounding pH dynamic distribution during electrochemical testing of steel pitting corrosion. First, an in-situ steel test sample with an observation surface is prepared and encapsulated, and then horizontally mounted in an adjustable electrolytic cell. Next, a methyl orange calibration solution with a pH gradient of 1-7 is prepared, and images of the sample at different pH values ​​are captured and imported into the system for calibration, establishing a quantitative mapping relationship between pH and the Lab values ​​of the calibrated images. Finally, in-situ electrochemical testing is conducted in a colorless salt test solution containing the same concentration of methyl orange, simultaneously monitoring the morphology of pitting initiation and development, as well as the dynamic evolution of the surrounding pH. This system can achieve millisecond-level dynamic in-situ monitoring of the spatial distribution of pH around pitting corrosion during the corrosion growth process, and can continuously capture real-time pH evolution information during the initiation and expansion of pitting corrosion.

Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and / or elution of fractions containing analytes.

This invention discloses a method and system for real-time pH control and stabilization in online column separation, belonging to the field of ion exchange chromatography separation technology. The method includes: applying a proton perturbation signal to the chromatographic column and detecting its elution response signal to obtain a parameter spectrum characterizing the dynamic buffer capacity of the chromatographic column; inputting the parameter spectrum and the target pH gradient into a physical transport model based on convection, diffusion, and adsorption coupling equations; the physical transport model outputs a pre-distorted pH gradient program to compensate for the dynamic buffer capacity characteristics; and based on the fluid ratio requirements defined by the pre-distorted pH gradient program and varying over time. This allows for the acquisition of dynamic buffer capacity characteristics through proton perturbation testing and the construction of a pre-distorted pH gradient program. Combined with closed-loop iterative optimization, this effectively counteracts the interference of the chromatographic column's dynamic buffer capacity on proton transport, ensuring that the pH curve of the post-column elution accurately matches the target linear gradient and eliminating pH nonlinear distortion and plateau problems.

The utility model relates to the technical field of water electrolysis hydrogen production, and discloses a pH gradient adjusting device which comprises an electrolysis tank, a liquid storage tank and a stirring barrel, the electrolysis tank is communicated with the liquid storage tank through a first water pump and a first water pipe, and the liquid storage tank is communicated with the bottom end of the stirring barrel through a second water pump and a second water pipe. One side of the top of the stirring barrel is communicated with a water inlet pipe, the water inlet pipe is externally connected with water supply equipment, the other side of the top of the stirring barrel is communicated with a feeding pipe, a quantitative discharging mechanism is arranged on one side of the feeding pipe, and a stirring mechanism is arranged in the stirring barrel. The weight of alkaline materials entering the stirring barrel can be controlled by controlling the number of times that the air cylinder drives the quantitative pipe to move back and forth, the amount of water entering the stirring barrel is controlled in cooperation with the liquid level sensor, the concentration of prepared alkaline liquor can be conveniently controlled by workers, and then the PH value in the electrolysis box can be conveniently controlled.

The invention belongs to the crossing field of synthetic biology and fermentation engineering, and relates to a lactobacillus brevis carbon source screening method based on a whole genome metabolism model. The method comprises the following steps: firstly, carrying out whole genome sequencing on a target strain, and constructing an initial model by utilizing gapseq; a representative cheap carbon source is used for carrying out a'two-stage sequential correction 'experiment, sugar transport and energy metabolism are constrained and corrected by a specific growth rate, and then GAD branch flux is constrained and corrected cooperatively by a glutamic acid-GABA conversion rate and a substrate cost, so that the model precision is improved. And then simulating a plurality of carbon sources in batches in the correction model, screening an optimal combination with high biomass, high GABA and low cost, and performing closed-loop verification through high-throughput micro-feeding and blind test experiments. And finally, the yield of the GABA is increased through'starvation-pulse 'material supplementation and pH gradient optimization, and a rapid, low-cost and rational carbon source screening process from a genome to process amplification is realized.

This invention discloses a two-stage enhanced gadolinium-based polymer magnetic resonance contrast agent with dual pH threshold response and its application. The contrast agent has an A- b -B-L-C(Gd), where A and B are anionic and cationic pH-sensitive polymer blocks, respectively, with the response threshold of block B being higher than that of block A; C(Gd) is the Gd-containing compound at the end of block B. 3+ Chelating groups. Contrast agents form first micelle states at physiological pH where water molecules have low contact ability with C(Gd). T 1. Low background signal; when pH is below the B-block response threshold, micelles depolymerize, significantly increasing the contact ability between water molecules and C(Gd), achieving first-order [response]. T 1. Enhancement: When the pH is below the A-block response threshold, the A-block response reassembles to form a second micelle state, maintaining the contact ability of water molecules with C(Gd) and prolonging the rotation time, thus achieving second-order [interaction / reaction]. T 1. Enhancement. This invention is applicable to pH gradient-responsive magnetic resonance imaging in acidic environments such as tumors, inflammation, and lysosomes. T 1. Imaging.