165results about How to "No choice" patented technology

An application of graphene in polymerase chain reaction as a reinforcing agent relates to a graphene material. An improved Hummers method is utilized to synthesize graphite oxide, and hydrazine hydrate is utilized to synthesize graphene. A plasmid is used as a template to carry out polymerase chain reaction and multi-round polymerase chain reaction, the graphene can increase the specificity of the polymerase chain reaction within 14 mu gmL-1, and a target product with strong specificity can even be obtained through 8-round polymerase chain reaction. A clinical blood sample deoxyribonucleic acid (DNA) is taken as a template to carry out the polymerase chain reaction, and a polymerase chain reaction (PCR) reaction system which is added with the graphene can obtain a product with single specificity and can still obtain the target product with the strong specificity within a scope between 25 DEG C and primer annealing temperature. The amplification products which are added with the graphene do not influence the primer sequence and length through sequencing tests, and the graphene can be used as a polymerase chain reaction reinforcing agent with good performance for polymerase chain reaction.

The invention discloses a double-layer thermal barrier/high-temperature low-infrared emissivity integrated coating which is of a multilayer superposition structure. The multilayer superposition structure sequentially comprises a metal bonding layer, a thermal barrier ceramic inner layer, a thermal barrier ceramic outer layer and a low-infrared emissivity layer from inside to outside, and the thermal barrier ceramic inner layer is La2Zr2O7-8YSZ mixture layer, wherein the mass fraction of La2Zr2O7 powder in the mixture is not more than 45%, the thermal barrier ceramic outer layer is a rare earthzirconate layer, and the low-infrared emissivity layer is a Bi2O3-Al2O3-ZrO2-CaO-SiO2 glass coating comprising conductive phase AuPt alloy powder. The invention also provides a metal composite material with the coating and a preparation method thereof. The integrated coating has the characteristics of heat insulation performance, high-temperature low infrared emissivity, excellent thermal shock resistance and the like.

The invention provides a surface enhanced Raman scattering substrate, which comprises a polylactic acid electrostatic spin film and nano metal particles on the surface or in the inner of the polylactic acid electrostatic spin film, wherein the nano metal particles carry positive charges. The surface enhanced Raman scattering substrate has the advantages of high enhancing ability, good repeatability, long storage period, and capability of enriching articles to be detected. The invention also provides a preparation method of the surface enhanced Raman scattering substrate. The preparation method comprises the following steps: (1) dissolving polylactic acid into an organic solvent to obtain a spinning liquid, and using the spinning liquid to carry out electrostatic spinning to obtain a polylactic acid electrostatic spin film; (2) arranging nano metal particles on the surface and in the inner of the obtained polylactic acid electrostatic spin film through a spotting mode so as to obtain the surface enhanced Raman scattering substrate. The preparation method is simple and is suitable for massive production. The prepared surface enhanced Raman scattering substrate can be applied to food analysis, environment analysis, etc.

The invention discloses a method for rapidly degrading antibiotics. According to the method, by utilizing an MIL-101(Fe)/TiO2 composite material as a catalyst, persulfate can be greatly catalyzed and activated under a normal temperature and weak light source to rapidly generate a large quantity of sulfate radical free radicals so as to degrade the antibiotics; a composite material is easy to recycle, can remain a relatively good activating effect after being repeated used and is a green environment-friendly material; according to the method, equipment is simple and convenient to operate, the energy consumption is saved, the catalysis time is short, and extremely high degradation effect on the antibiotics can be achieved under relatively weak natural light in severe-haze and light-deficient areas; and the method has great application prospects in the degradation of the antibiotics.

The invention discloses a capacity increasing method for a nano particle modified carbon fiber sizing agent. The capacity increasing method comprises steps as follows: (1), nano particles, POSS (polyhedral oligomeric silsesquioxane) and a catalyst are dissolved in an organic solvent and subjected to ultrasonic treatment for 5-20 min, and a nano particle dispersion liquid is obtained; (2), the nano particle dispersion liquid in the step (1) is uniformly mixed with epoxy resin and an emulsifier, an organic solvent in the mixture is removed through distillation under reduced pressure, and a corresponding sizing agent emulsion is prepared with a phase inversion method. POSS and the nano particles are introduced into the sizing agent simultaneously, advantages of POSS and the nano particles can be combined, and the problems of poor compatibility and high probability of agglomeration and demulsification of the nano particles in the sizing agent can be solved; besides, in comparison with traditional coating modification and coupling agent modification, the treatment method adopting POSS as a capacity increasing agent is simple, and more excellent heat resistance is acquired.

The invention relates to a rock core shape-preserving three-dimensional reconstruction method. The method comprises the steps that holographic image information of the contour of a rock core is obtained through multi-angle shooting of a calibrated measurement camera, and then a high-density point cloud on the surface of the rock core is obtained by means of scanning analysis of a high-performance computer on images according to the dense curve modeling technology; gridding and automatic filling of the surface of the rock core are completed through effective determination of the point cloud, and therefore the three-dimensional reconstruction of the rock core is completed. Compared with a traditional rock core characterization method, a large amount of three-dimensional information of the rock core with the original shape can be obtained instantly, no rock core shape selectivity exists, no special handling is needed, the original shape of the rock core can be well kept, the cost is low, operation is convenient and fast, the degree of automation is high, on-site quantitative analysis can be carried out on a coring site, the defects of existing methods are effectively overcome, and the rock core shape-preserving three-dimensional reconstruction method is efficient, fast, low in cost, accurate and reliable.

The invention relates to a method for cleanly extracting vanadium pentexide from a vanadium containing material. The method is characterized in that: the vanadium containing material is milled by the wet method, and then mixed with a certain weight amount of sulfuric acid, vanadic acid, water, sulfuric acid or phosphoric acid salt having certain association; under conditions of set temperature and set time, performing three-stage reflux leaching reaction of the mixture; adding ammonia sulfate and reducing iron powder to reaction leach solution in sequence to obtain aluminum ammonium sulfate crystals and ammonium ferrous sulfate crystals respectively; adding sulfuric acid to the solution in which aluminum and iron are separated out, cooling the solution for crystallization to obtain vanadium sulfate acyl crystals, and returning the mother liquor to extract vanadium ores; performing the neutralization reaction of the vanadium sulfate acyl crystals and ammonia water to obtain a precipitate of vanadium hydroxide, and returning the solution of ammonia sulfate to precipitate the aluminum and iron; and calcining the vanadium hydroxide and oxygen gas at a high temperature of over 300 DEG C to obtain the vanadium pentexide. The method has the advantages of short process flow, less energy consumption, high vanadium yield, low production cost, environmental protection, and comprehensive utilization of valuable metallic elements in ores.

The present invention relates to a gas leakage monitoring device based on a background schlieren technology, and a gas leakage monitoring method based on the background schlieren technology. According to the present invention, based on the background schlieren technology, by comparing the two background photographs which are acquired at the different times or are acquired at the same time but at the different angles, and by the displacement of a lot of points on the photographs, the gradient change of an air refractive index in an area is detected, an air refractive index gradient change area caused by the gas leakage is identified, and the gas leakage is determined whether to occur, thereby determining the position of a leakage point. The device and method of the present invention are simple in structure, can monitor the gas leakage and search the leakage point in a large-range open environment and a complicated pipe network system, do not have selectivity to the gas types, and can monitor the all gas leakage.

The invention provides an organic waste water ozone oxidation catalyst and a preparation method and application thereof. The organic waste water ozone oxidation catalyst comprises the following raw materials in parts by mass: 0.6-1.2 parts of active metals, 20-26 parts of titanium dioxide, 41-45 parts of aluminum oxide, 14-20 parts of manganese dioxide and 8.2-9.6 parts of activated carbon, wherein the active metals comprise titanium, copper, zinc, nickel and manganese. The mass ratio of titanium, copper, zinc, nickel and manganese is 1.2:3:2:1.6:2.2.

The invention discloses a DNA library construction method for high-throughput sequencing. The DNA library construction method includes the steps of carrying out multiple PCR (polymerase chain reaction) to a DNA template obtained from a to-be-tested sample through multiple pairs of fusion primers, then recycling PCR products to obtain a sequencing library. The multiple pairs of fusion primers respectively aim at different target fragments of the DNA template, and each fusion primer comprises specificity primer sequence aiming at the target fragment and linker sequence for sequencing. The multiple PCR is carried out on reaction conditions: reacting at 95 DEG C for 2 minutes; reacting within 38 cycles, wherein each cycle includes processes of preserving at the temperature of 95 DEG C for 30 seconds, cooling to the temperature from 76 DEG C to 55-58 DEG C slowly at the speed of 0.1 DEG C each second, holding the temperature for 20 seconds once cooling to the temperature of 58 DEG C, and then preserving at 72 DEG C for 30 seconds; after that, preserving at 72 DEG C for 2 minutes, and finally preserving at 4 DEG C. The invention further discloses application of the library construction method in testing STR locus and paternity identification on the basis of high-throughput sequencing.

The invention discloses a composite flocculant for treating printing and dyeing wastewater. The composite flocculant for treating the printing and dyeing wastewater is prepared from the following rawmaterials in parts by weight: 15-25 parts of activated carbon fibers, 4-11 parts of acrylamide, 2-9 parts of sodium carboxymethyl cellulose, 5-17 parts of polyvinyl alcohol, 3-6 parts of a crosslinking agent, 0.003-0.006 part of an initiator, 3-5 parts of a polyethyleneimine-cellulose composite material, 10-15 parts of starch xanthate and 25-36 parts of modified bentonite. The composite flocculantprovided by the invention does not contain components which are complicated, have radiation and easily cause secondary pollution, all the used raw materials are natural, environment-friendly, low inprice and easy to obtain, the preparation method is simple, the feeding amount is small, the composite flocculant has multiple effects of flocculation sedimentation, adsorption decolorization, deodorization and the like, the sedimentation rate is high, the removal rates of organic matters, dye molecules and heavy metals are high, the structure and properties are stable, and the application range is wide.

The invention discloses a shielding optical window and a preparation method thereof. The method comprises the steps of (1) cleaning a substrate; (2) coating the substrate with a protective layer; (3) etching the protective layer and the substrate; (4) carrying out sputtering coating on the surface of the etched substrate; and (5) removing the protective layer to obtain the shielding optical window. According to the method for preparing the shielding optical window, metal precipitation is not required, and the material of the substrate is not required.

The invention discloses an air purifier. The air purifier comprises a purifier casing, an air inlet fan, a filter net, a water mist generator, an electrostatic device and a sewage storage box, wherein the air inlet fan is mounted on the inner side of an air inlet or an air outlet of the purifier casing; the filter net comprises a front filter net body and a back filter net body which are mounted on inner sides of the air outlet and the air inlet of the purifier casing respectively, and the front filter net body, the back filter net body and the purifier casing form an airtight cavity; the water mist generator is mounted at the bottom of the airtight cavity, is used for generating small water mist particles with high concentration and enables the cavity to be full of the particles; the electrostatic device is mounted in the purifier and matched with the water mist generator to enable the water mist particles to be charged; the sewage storage box is mounted at the bottom of the purifier. The air purifier has the benefits as follows: the filtering effect is good; charged water mist is collected into the sewage storage box after adsorbing dust, and the air purifier is easy to clean and convenient to use; no consumable is used, core filter components are not required to be replaced frequently, and the using cost is reduced.

The invention discloses a high-pressure packaging container, and a high-pressure in-situ gas compression packaging and pressure monitoring system. The system comprises the high-pressure packaging container, a high-pressure in-situ gas compression packaging system and a high-pressure in-situ gas monitoring system, wherein the high-pressure in-situ gas compression packaging system can package both a plurality of inert gas pressure transmission media and a plurality gaseous-state samples; the high-pressure in-situ gas monitoring system can precisely calibrate ultrahigh pressure; the high-pressure packaging container is provided with an optics observation window and a high-pressure gas inlet, so that high pressure generated during the packaging process of the gaseous-state samples or the inert gas pressure transmission media can be monitored, the situation that pressure in the high-pressure packaging container is stable and controllable during the packaging process is ensured, and the problems that the packaging of the gaseous-state samples or the inert gas pressure transmission media fails caused by uncontrollable pressure in the high-pressure packaging container and a diamond anvil cell press is damaged are avoided to further increase the success rate of a high-pressure in-situ experiment.

The invention relates to the field of fungi molecular biology, in particular to an instrument-free yeast-like fungus DNA extraction method used for nucleic acid amplification. According to the method, two yeast wall breaking methods, namely, the chemical lysis method and the enzymatic lysis method, are ingeniously integrated, DNA extraction is completed within 1.5-3 hours through the combination with a suction device, a filter device and a nucleic acid extraction syringe without power supply devices, and the method is the only efficient instrument-free yeast-like fungus DNA extraction method at present. The extraction method has the advantages of being rapid, simple, convenient to use, sensitive, free of toxin, low in cost and the like, and can be suitable for most yeast-like fungi.

Provided is a novel use of formaldehyde in preparing pharmaceuticals for prevention and cure of animal diseases caused by pathogenic microorganisms through infection of digestive canal by means of digestive tract administration, wherein formaldehyde can deactivate pathogenic microorganisms and their toxins, make it lose pathogenicity while keeping the antigenicity. The invention realizes the advantages of broad spectrum, quick and sure effects, small dosage, less administration, lower price and removal of drug resistance and medicinal residual produced by the application of antibiotics.

The invention discloses a powder type blattaria bait. The bait agent comprises the components of pesticide original medicine, a composite feeding stimulant, a preservative agent and a filling agent, wherein by weight percent, the pesticide is 1.0-3.0 percent, the composite feeding stimulant is 80.0-85.0 percent, the preservative agent is 0.5 percent and the filling agent is the rest. The bait has the characteristics of inexpensive materials, simplicity in manufacturing, convenience in scattering and good population control effect, and has no harm to human, domestic animals and the environment.

The invention relates to the technical field of molecular biology and particularly relates to a novel methylation library-establishing kit and an application thereof. The novel methylation library-establishing kit at least comprises a bisulfate conversion reagent, a sticky end joint, ligase, a DNA extending reagent and a DNA amplifying reagent. The novel methylation library-establishing kit can beapplied to a sample with a low initial mass (as low as 6pg) and suitable for genome DNA with relatively poor mass extracted from FFPE samples and the like, so that the loss of sample information caused by DNA breakage during drug treatment is greatly reduced; and a double-chain DNA joint with a sticky end can be directly introduced by virtue of relatively economic and practical common ligase, sothat the accuracy of data can be improved, the diversity of samples can be preserved to a great extent, and the library deviation property caused by human factors can be reduced.

The invention relates to an amorphous tellurium-cadmium-mercury/crystalline silicon heterojunction infrared-detector, which is characterized by being composed of a crystalline silicon substrate, amorphous tellurium-cadmium-mercury, a first metal electrode and a second metal electrode, wherein the amorphous tellurium-cadmium-mercury and the crystalline silicon substrate form heterojunction, the first metal electrode is connected with the amorphous tellurium-cadmium-mercury, and the second metal electrode is connected with the crystalline silicon substrate. The preparation process comprises the following steps of substrate cleaning, amorphous tellurium-cadmium-mercury film preparation, photosensitive surface forming, metal electrode preparation and package test; or substrate cleaning, second metal electrode preparation, amorphous tellurium-cadmium-mercury film preparation, photosensitive surface forming, first metal electrode preparation and package test. The amorphous semiconductor material has no selectivity for the substrate, the lattice matching performance between materials forming heterojunction is better, and the significant photoelectric response exists. The optimum working temperature of the detector is close to room temperature, two stages of semiconductors are utilized for refrigeration, and the weight, the power consumption and the manufacturing cost of the infrared detector assembly are reduced.