308results about How to "Good cracking effect" patented technology

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg/ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10% of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg/ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.

The invention discloses a method for extracting and purifying spittle DNA, which comprises the following: 1) a step of pre-treatment, which is to contact the spittle with pretreatment lysis solution, remove solid matters adhered onto cells and obtain coarse lysis solution; 2) a step of nucleic acid absorption, which is to mix the coarse lysis solution obtained by the step 1) with lysis binding solution and magnetic bead suspension to form a solution system containing magnetic nano microsphere and DNA composite and is to collect the magnetic nano microsphere and DNA composite from the solutionsystem; and 3) a step of washing, which is to wash the magnetic nano microsphere and DNA composite obtained by the step 2) with washing liquid I and washing liquid II in turn, and dissolve out DNA byusing eluent. When the method provided by the invention is used, the DNA extraction efficiency may reach 90 percent, the extracted DNA can be used in short tandem repeat (STR) composite amplification, DNA sequencing, DNA quantification and other downstream analysis and operation.

The invention discloses a method for extracting purified DNA from human exfoliative cells. The method comprises the following steps: 1) pretreating: contacting the human exfoliative cells with pretreating lysate to remove the solid matters adhering to the biological cells to obtain coarse lysate; 2) nucleic acid adsorbing: mixing the coarse lysate obtained in the step 1) with lysis integrated liquid and magnetic bead suspension to form a solution system containing a magnetic nano microsphere-DNA compound and collecting the magnetic nano microsphere-DNA compound in the solution system; and 3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step 2) with washing liquid I and washing liquid II respectively and then dissolving out the DNA with eluent. The efficiency of extracting DNA by the method can reach 90% and the extracted DNA can be applied to such downstream analysis operations as STR (short tandem repeat) multiplex amplification, DNA sequencing, DNA quantifying and the like.

The invention discloses a disc-type microwave continuous splitting device. The disc-type microwave continuous splitting device comprises a feeding assembly, a microwave splitting assembly, a discharging assembly and a control assembly which are sequentially communicated with one another, wherein the microwave splitting assembly comprises a microwave generator, a nonmetal sealing plate, a splitting cavity, a rotating disk and a disk driving motor, the nonmetal sealing plate is used for dividing the splitting cavity into an upper part and a lower part, the rotating disk is arranged at the lower part of the splitting cavity and is matched with the disk driving motor in a driving rotation manner, and the microwave generator is arranged on a circular flange on the splitting cavity. The invention aslo discloses a method for splitting waste rubber by using the splitting device; the disc-type splitting cavity is adopted in the method and is divided into an upper cavity and a lower cavity through the nonmetal sealing plate, so that the microwave splitting efficiency of waste tires is improved; and in order to ensure the stable operation of a disk and prevent the high-temperature deformation, one or a plurality of annular guide rails are arranged on the lower surface of the rotating disk and are used for supporting the rotating disk, so that the rotating disk rotates on a guide rail so as to optimize the splitting effect.

The invention relates to the field of biology, particularly to novel Staphylococcus aureus phages and a composition thereof, and applications of the composition, and provides 4 Staphylococcus aureus phages such as Podoviridae sp .BP-13 with the preservation number of CCTCC NO:M2015142, Staphylococcus aureus phage BP-13A with the preservation number of CCTCC NO:M2016535, Podoviridae sp .BP-14 withthe preservation number of CCTCC NO:M2015143, and Podoviridae picovirinae Ahjdlikevirus BP-39 with the preservation number of CCTCC NO:M2015144. According to the present invention, the four phages arethe strict potent phages, can effectively kill various types of Staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have wide host ranges, and have no toxic effect on normal microbial flora, and the DNA of the phages cannot encode virulence genes, such that the Staphylococcus aureus phages can be used for preventing or treating human and animal bacterial infections caused by Staphylococcus aureus, can provide excellent strain resources for the development of novel antibacterial preparations, and have good application development prospects; and the host range and the antibacterial efficiency of the cocktail mixture of the present invention are further greatly improved.

The invention discloses a shell technology based software protection method, and relates to the technical field of software encryption. Based on the mechanism of code disorder, the software protection method includes the steps of resequencing codes of an original program and a shell program so as to achieve the purpose of integration, while adding junk codes to defense static decompile; and utilizing an SHE (Super Hybrid Engine) technology in the shell program to prevent dynamic trace. When a shell of the software protected by the protection method is cracked off by a cracker, part of the original program is also removed, and accordingly the object of software protection is achieved.

The invention discloses high-carbon non-tempered steel for breaking a connecting rod and a manufacturing method thereof. The high-carbon non-tempered steel comprises the following chemical elements in percentage by weight: 0.65%-0.85% of C, 0.15%-0.25% of Si, 0.50%-0.60% of Mn, 0.010%-0.045% of P, 0.060%-0.070% of S, 0.10%-0.20% of Cr, 0.55%-0.80% of Ni, 0.03%-0.04% of V, 010%-0.20% of W, 0.0120%-0.0160% of N, 0.0001%-0.0004% of Re and the balance of Fe and inevitable impurities. The manufacturing method comprises the following steps: electric furnace primary smelting; ladle refining and vacuum degassing; die casting or continuous casting; and steel bar rolling. The high-carbon non-tempered steel has relatively high strength and good breaking performance.

The invention relates to the field of food safety, and particularly discloses a broad-spectrum salmonella phage LPSTLL and application. The preservation number of the salmonella phage LPSTLL is CCTCCNO: M 2019049. The salmonella phage LPSTLL is a virulent phage, has a very broad host spectrum and can lyse 13 kinds of serotype salmonellae such as salmonella typhimurium, salmonella enteritidis andsalmonella dublin and lyse a plurality of strains of drug-resistant salmonellae. The invention further discloses bacteriostatic application of the phage as a biological control agent in food storage.The phage particularly can inhibit contamination caused by the proliferation of salmonellae in milk and chicken, and therefore the safety and hygiene of food are ensured.

The invention provides a color image compression and encryption method based on compressed sensing and a double random encryption mechanism. The method comprises the following steps: firstly, decomposing a plaintext image into R, G and B components; the method comprises the following steps: firstly, obtaining a sparse coefficient matrix, then respectively performing DWT conversion on the sparse coefficient matrix, then performing double random position scrambling on the sparse coefficient matrix by using index vectors D1, D2, D3 and D4, then performing compression measurement and quantification on the scrambled matrix, performing simultaneous double random pixel value diffusion among R, G and B components and inside the components on the quantified matrix, and finally obtaining a ciphertext image. Parameters and initial values of a four-wing hyper-chaotic system and a Logistic-Tent chaotic system are generated by using plaintext image information and an external key, and then the parameters and the initial values are substituted into the Logistic-Tent chaotic system to generate a chaotic sequence to construct a measurement matrix; and then substituting the parameters and the initial values into a four-wing hyperchaotic system, and sorting the four generated chaotic sequences to obtain four index vectors which are respectively used for double random position scrambling and diffusion.

The invention provides an extruding and rubbing type walnut shell breaking device which comprises a box body and a rack. The box body is arranged on the rack. A feeding port is arranged on the top surface of one side of the box body. A discharging port is arranged on the bottom surface of the other side of the box body. A conveyer and a shell breaker are arranged inside the box body. The conveyer is located at a horizontal position between the feeding port and the discharging port and used for conveying walnuts which fall into the conveyer from the feeding port to the discharging port. The shell breaker is located above the conveyer and used for breaking shells of the walnuts conveyed by the conveyer. When the extruding and rubbing type walnut shell breaking device is used, the walnuts are placed into the feeding port, fall onto a conveying chain due to gravity, and rotate along with the conveying chain; the walnuts are extruded by a pressing plate and rubbed by rollers, so that it is guaranteed that the shells of the walnuts are completely broken. According to the extruding and rubbing type walnut shell breaking device, the distance between the pressing plate and the conveying chain can be adjusted according to the sizes of the walnuts, the walnuts are rubbed in the extrusion process so that the shells of the walnuts are completely broken, and therefore the extruding and rubbing type walnut shell breaking device has the advantages of being high in work efficiency, shell breaking rate and perfect kernel rate, low in cost and convenient to use.

The invention discloses an automatic energy storing high-pressure liquid injecting CO2 phase change pulse coal and rock body cracking method. Liquid state CO2 enters a high-pressure liquid injecting cylinder and a phase change chamber in the mode that the liquid state CO2 is injected into a high-pressure liquid storing temperature control tank, the liquid state CO2 in the phase change chamber is heated, the phase change pressure is increased, and gaseous CO2 enters the energy storing chamber to be subjected to automatic energy storing and a coal rock cracking chamber to be cracked; and when the pressure of the CO2 gas in the phase change chamber is decreased, the high-pressure CO2 gas enters the high-pressure liquid injecting cylinder to push a piston to expel the remaining CO2 gas, and enters the coal rock cracking chamber to be subjected to second-time cracking, the circulation of liquid injection, oil bath heating, phase change, energy storing and cracking is repeated, and continuous pulse high pressure flow jetting cracking is realized. According to the method, continuous pulse high pressure flow jetting cracking is realized, the time of cracking action is prolonged, the range of cracking action is expanded, the cracking effect is improved, and the method is suitable for quick and efficient cracking of solid materials such as coal, rock, concrete, soil and congelation.

The invention discloses a low-temperature cracking and caving method of a hard roof in a coal mine goaf. The low-temperature cracking and caving method is suitable for the technical field of strengthening cracking of roofs in the coal mine roaf, and comprises the steps that a digital display thermograph, temperature sensors, a water injection pipe, a low-temperature fluid freezing pipe, a capsulehole sealer, a high-pressure water injection system and a low-temperature fluid tank car are utilized to form a plurality of cracking drill holes and an empty hole in the hard roof in the goaf sequentially; the spiral low-temperature fluid pipe, the water injection pipe and the capsule hole sealer are connected and sent into the multiple cracking drill holes, the capsule hole sealer is adopted forhigh-pressure hole sealing, cylindrical expanding sections of hole openings of the cracking drill holes are frozen to form a self-hole-sealing structure, then the multiple cracking drill holes are frozen, and the coal seam roof is cracked by water-ice phase transformation; and when the temperature sensors all rise above 3 DEG C, freezing is conducted repeatedly, and the cycle of cracking of frostheaving, melting and frost heaving is formed. The water-ice phase transformation is conducted by the low-temperature fluid to generate an expansion rate of 9.1 % to crack the coal seam hard roof, themethod is simple, operation is convenient, the cost is low, operation is easy, and the cracking effect is good.

The invention discloses a salmonella phage LPSE34. The preservation number of the salmonella phage LPSE34 is CCTCC NO 2018121. The phage is a broad-spectrum salmonella phage and can crack drug-tolerant salmonellas, it is identified that the phage belongs to myoviridae, and the phage is named as LPSE34. The phage LPSE34 is stable in titer at the pH of 4-12 and the temperature of 30-60 DEG C. The invention further discloses application of the salmonella phage in food, especially in chicken and ham systems; and in addition, the invention further discloses a compound edible coating film formed bytaking the phage as an antibacterial substance to be combined with sodium alginate, and compared with antibiotics and chemical preservatives, the phage cannot affect the texture and flavor of food andhas the characteristics of high specificity, no residue and safety. The salmonella phage LPSE34 can serve as a bacteriocidal substance/the antibacterial substance to control pollution of food-borne pathogens to the food and is a very promising biological agent for guaranteeing the food safety.

The invention relates to a converter steel slag treatment new process. The converter steel slag treatment new process comprises the technological steps that (1) steel slag hot splashing: hot-melting steel slag is directly put into hot splashing slag tanks and is subjected to water cooling to make the steel slag and water have a reaction; the steel slag is subjected to self-decomposing and powdering, and slag and iron are separated; (2) the powdered steel slag subjected to hot splashing is grabbed and transported to a self-discharging transportation truck by an excavator and transported to a steel slag screening, crushing and magnetic separation production line; (3) the steel slag is screened through a grid screen with 200-mm screen holes below a hopper, and the steel slag with the grain size greater than 200 mm is loaded onto the self-discharging truck through a loader and transported to a drop hammer crushing room for crushing, the drop hammer crushing room is equipped with electromagnetic suction cups and drop hammers, crushed steel slag is returned for steelmaking, and the steel slag returns to the grid screen; and (4) the steel slag with the grain size less than 200 mm is subjected to a series of crushing, and screening and then are directly returned for steelmaking, the steel slag with the grain size less than 10-50 mm is fed into a rod mill for crushing and stripping andis subjected to magnetic separation twice through an electromagnetic self-discharging iron separator and a roller magnetic separator, and magnetic separated powder with the grain size less than 10 mmand tailings with the grain size less than 10 mm are fed into a magnetic separated powder warehouse and a tailing warehouse correspondingly. Through the converter steel slag treatment new process, thequality level of the steel slag TFe reaches to 85% or above finally, and the iron content of the tailings is less than 2%.

The invention discloses a high-efficiency nut shelling machine which mainly comprises a nut fetcher, a machine frame, a fixed blade, a movable blade, a movable knife rest, a motor and a gearbox, wherein the fixed blade is horizontally arranged on the machine frame; the movable knife rest is arranged on the machine frame; the movable blade arranged on the movable knife rest corresponds to the fixed blade; a certain gap is formed between the movable blade and the fixed blade; the nut fetcher mainly comprises a nut fetching roll, a hair brush roll, a guide hopper and a nut box; the motor is used for transmitting power to the gearbox; the gearbox is used for driving the movable knife rest to reciprocate through a transmission mechanism and driving the nut fetching roll to intermittently rotate at the same time; when the nut fetching roll rotates at a certain angle each time, a row of nuts in a nut receiving groove fall into the guide hopper and fall into the gap through the guide hopper. A cam is matched with a return spring to drive the movable knife rest to reciprocate for driving the movable blade to shell the nuts. By adopting the structure form, the shelling efficiency is improved and the shelling effect is very good.

The invention discloses a coal and rock mass fracturing device adopting automatic energy storage high-pressure liquid injecting and CO2 phase change pulse. The device is composed of a liquid supplying system, an automatic energy storage high-pressure liquid injecting system, a phase change fracturing system and a datum collecting and control system. The liquid supplying system comprises a liquid state CO2 source, a liquid booster pump and a high-pressure liquid storage temperature control tank; the automatic energy storage high-pressure liquid injecting system comprises a high-pressure liquid injecting cylinder, an energy storage chamber and a phase change chamber; the phase change fracturing system comprises a phase change chamber and a fracturing body; and the datum collecting and control system comprises pressure sensors, temperature sensors, stop valves, safety valves and a control computer. Continuous pulse high-pressure jet flow fracturing is achieved through automatic circulation of the process of liquid injecting, oil bath heating, phase change, energy storage and fracturing, and the coal and rock mass fracturing device is suitable for quick and efficient fracturing of solid materials such as coal, rock, concrete, soil and congelation.

Belonging to the technical field of solid waste treatment, the invention relates to a method for promoting anaerobic digestion of excess sludge through pretreatment of ultrasonic waves and hydrogen peroxide. The method comprises: subjecting the excess sludge of an urban sewage treatment plant to gravity thickening, then adding a certain amount of hydrogen peroxide, conducting rapid stirring and sending the sludge into an ultrasonic reactor for ultrasonic treatment, in which the combined action of ultrasonic waves and hydrogen peroxide destroys the bacterial cell wall structure of the sludge, thus leading to leakage of the cell contents and content increase of dissolved organic matters in water, then sending the treated sludge into an anaerobic digestion reactor for subsequent mesophilic anaerobic digestion; delivering the biogas generated from anaerobic digestion to a gas collection gas for comprehensive utilization. After treatment, the concentration of SCOD (soluble chemical oxygen demand) in the liquid phase sludge can be increased by 23.83 times to 41.18 times, the biogas output can be increased by 17.40%-55.37%, and the methane content of the biogas can be increased by 10%-20%, thus reducing the energy consumption of ultrasonic treatment. For sewage treatment plants of different scales, only by making corresponding modification on existing facilities can good effects be achieved.

The invention relates to a method for utilizing enhanced pretreatment to promote the anaerobic digestion of excess sludge, belonging to the technical field of solid waste treatment. The method comprises the following steps of gravity concentrating excess sludge in urban sewage treatment plants, and then adding certain amount of sodium hypochlorite, stirring the sodium hypochlorite fast with the excess sludge, and then feeding the mixture into an ultrasonic reactor to perform ultrasonic treatment. With the coaction of the ultrasonic and the sodium hypochlorite, the bacterial cell wall structure in the sludge is damaged, the inclusion of the cell is discharged, the content of soluble organic substance in the water is increased, after certain time of treatment, the sludge is fed into an anaerobic digestion reactor to perform subsequent medium temperature anaerobic digestion; the biomass gases generated by the anaerobic digestion are comprehensively utilized through a gas collection device. The concentration of SCOD (soluble chemical oxygen demand) in the sludge liquid phase is improved by about 27 times after the treatment, the biomass gas yield is increased by 54.4%, and the content of methane in the biomass gas is improved by about 10%, so that the energy consumption of the ultrasonic treatment is reduced greatly. With the adoption of the method provided by the invention, for the sewage treatment plants with different sizes, a good effect can be obtained by performing corresponding reconstructions on the existing treatment facilities.

The invention relates to a carbon dioxide phase-change coal seam cracking guiding perforating device and an outburst-preventing and scour-preventing method. The carbon dioxide phase-change coal seam cracking guiding perforating device comprises a carbon dioxide phase-change coal seam cracking instrument and is characterized in that a perforating bullet is arranged in an explosion venting hole of the carbon dioxide phase-change coal seam cracking instrument and the explosion venting hole is plugged by a plug. The invention also provides the outburst-preventing and scour-preventing method. The device combined with the outburst-preventing and scour-preventing method provided by the invention is used for guiding the high energy gas through a perforating bullet track, so that the cracking effect is better; the orientation arrangement is adopted, so that the permeability is added to the perforating bullet track; the blast hole and the control hole are arranged, so that the cracks surrounding the blast hole and the control hole are more effectively communicated with each other; the cracking area is increased due to the fan-shaped arrangement.

The invention discloses a method for extracting and purifying semen DNA. The method comprises the following steps of: (1) pretreatment: keeping semen in contact with a pretreatment lysis solution and removing solid substances adhered to biological cells to obtain a crude lysis solution; (2) nucleic acid adsorption: mixing the crude lysis solution obtained in the step (1) with a lysis binding solution and a magnetic bead suspension solution to form a solution system containing a magnetic nano microsphere-DNA compound, and collecting the magnetic nano microsphere-DNA compound in the solution system; and (3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step (2) by sequentially using a washing solution I and a washing solution II, and dissolving DNA out with eluent. According to the method provided by the invention, the DNA extraction efficiency can reach 90 percent, and the extracted DNA can be applied to STR (Short Tandem Repeat) composite amplification, DNA sequencing, DNA quantification and other downstream analysis operations.

The invention relates to the technical field of code cracking, and discloses a code cracking method based on multiple GPU cracking devices. The code cracking method based on the multiple GPU cracking devices includes the following steps that firstly, a client side builds a cracking task according to the user requirement and sends the cracking task to a GPU proxy device, and the GPU proxy device divides the cracking task sent by the client side into a plurality of sub-tasks and distributes the sub-tasks to the different GPU cracking devices; secondly, each GPU cracking device executes the distributed sub-task and feeds the cracking result back to the client side through the GPU proxy device. The invention further discloses a code cracking system based on the multiple GPU cracking devices. The code cracking method and system can support a multi-node GPU cluster, properly carry out expansion of the GPU cracking devices according to requirements, improve the code cracking speed and well improve the code cracking performance.

The invention provides a process for producing fuel oil by thermal cracking of biomass and relates to the technical field of thermal cracking of biomass and renewable resources. The process mainly comprises the following steps: preparing biomass and waste plastics used as raw materials; carrying out thermal cracking on the raw materials in a thermal cracking furnace; carrying out solid-gas separation, wherein the non-condensable gas is used as a heat resource for a thermal cracking reaction; enabling a heat carrier to be maintained in the furnace to continuously participate in the reaction; esterifying, distilling and catalyzing biological crude oil and condensing to obtain the biofuel products. By virtue of the process disclosed by the invention, the defects of the prior art are overcome, the catalytically refined fuel oil has the characteristics of high calorific value, good liquidity and good stability; the process is reasonable, simple in control, convenient, energy-saving, high in production efficiency, especially strong in suitability for raw materials, simple in equipment and convenient to operate.

The invention discloses a method for extracting total DNAs of soil microorganisms at high purity. CTAB (Cetyltrimethyl Ammonium Bromide), lysozyme, protease and SDS (Sodium Dodecyl Sulfonate) act together to perform lysis of cells and simultaneously freeze and thaw the cells repeatedly; therefore, an excellent cell lysis effect is achieved; the humic acid is removed by utilizing PVP (Polyvinyl Pyrrolidone), CaC12 and BSA (Bull Serum Albumin); and the DNAs are precipitated by utilizing isopropanol so that the purity of the obtained DNAs is high. The method is simple and convenient; and without being purified, the DNAs obtained by the method can be used for subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (Denaturing Gradient Gel Electrophoresis) analysis. The purity of the extracted DNAs meets the requirement for directly performing subsequent molecular biological research; the extracted DNA segments can be subjected to 16SrDNA amplification and DGGE atlas analysis; glue cutting recovery and basic group sequencing can be performed on specific stripes of the DGGE band; unknown bacteria can be identified through comparison with the conventional sequences in an RDP (Ribosomal Database Project) database or by establishing a new sequence probe; and therefore, the structural composition of the microbial community can be determined, and a foundation can be laid for researching the structural diversity and ecological functions of the soil microorganisms in the rhizosphere of the mulberry in future.

The invention relates to a reduction method and a reduction system for processing waste plastics. The method and the system take an IGBT heating module as the core, and can save over 40 percent of energy compared with the prior vacuum tube high-frequency equipment and the prior thyristor induction heating equipment. The method and the system adopt the novel catalytic pyrolysis method, namely the prior mode of directly placing catalyst zeolite into the waste plastics is changed and the waste plastics are gasified into oil gas and then degraded by Y-shaped molecular sieves. Therefore, the pyrolysis effect of the method and the system is more obvious; the quality of liquid fuel gasoline and diesel fuel produced is better; and the gasoline and the diesel fuel produced reach or exceed the national standards of No.70 gasoline SHO 42-92 and No. 0 diesel fuel GB 252-94. Moreover, No. 90 to No. 93 unleaded gasoline can be modulated by utilization of the gasoline.