4results about How to "Good cracking effect" patented technology

The application provides a kind of preparation of red radish protoplast enzymatic hydrolysate and the preparation method and application of red radish protoplast, belong to the technical field of plant cytology.The enzymatic hydrolysate includes:1.0~1.5w / v% cellulase, 0.3~0.5w / v% dissociation enzyme and 0.3~0.5w / v% hemicellulase.The cellulase has the effect of degrading cellulose in plant cell wall, dissociation enzyme is used with cellulase, which is beneficial to separate plant tissue into single cell, and the main effect of hemicellulase is to degrade hemicellulose in plant cell wall.The enzymatic hydrolysate can synergistically promote the effect of red radish tissue cell wall lysis through the comprehensive effect of three enzymes, can better and faster destroy cell wall skeleton, is more conducive to the preparation of red radish protoplast and further maximally release protoplast, is conducive to improving the yield of protoplast, and can also keep the protoplast with high activity.

This invention discloses a method for disrupting the cell walls of structurally heterogeneous microorganisms based on the synergistic vibration of multi-size media and its application. The method includes the following steps: (1) constructing a composite cell-dispersing media system with multi-size characteristics and filling it into a cell-dispersing tube; (2) injecting the microbial sample to be disrupted into the cell-dispersing tube and oscillating it at a linear velocity of 6-7 m / s for 30-90 seconds at 4-25 ℃; (3) after cell disruption, separating the mixed composite cell-dispersing media system and the solution to obtain a cell-dispersed solution containing intracellular products. The beneficial effects of this invention are: through the synergistic effect of multi-scale shear forces, it significantly improves the lysis efficiency of difficult-to-disrupt pathogens such as Mycobacterium tuberculosis, Cryptococcus, and Aspergillus, effectively protects the nucleic acid integrity of RNA viruses, has high cell disruption uniformity, good RNA protection, can be standardized and adapted to automated platforms, and is suitable for the pretreatment of complex clinical samples.

The application relates to the walnut processing technical field and discloses a walnut ultrasonic shell breaking and kernel taking device, which comprises a rack, a walnut conveying mechanism arranged on the rack, a walnut shell breaking mechanism and a material collecting mechanism. The walnut conveying mechanism is used for driving the fallen walnuts to move and is conveyed to the walnut shell breaking mechanism. The walnut shell breaking mechanism drives the walnuts to move and extrudes the walnuts to break the shells through ultrasonic high-frequency vibration. The material collecting mechanism is used for screening and collecting the walnut kernels and the walnut shells after the shells are broken. The walnut conveying mechanism, the walnut shell breaking mechanism and the material collecting mechanism realize the processes of walnut conveying, extrusion shell breaking and recovery. The ultrasonic wave is applied to the walnut shell breaking field. The walnut shells in Yunnan are hard and have small kernel-shell gaps. The high-frequency vibration under the mechanical effect of the ultrasonic wave is suitable for the characteristics of hard and brittle material breaking, assists the walnut shell breaking, and improves the shell breaking rate and the whole kernel rate.

This invention belongs to the field of biological sample detection technology, specifically relating to a lysis cell. This invention provides a lysis cell designed to solve the problem in existing lysis cells where the liquid and reagent cannot be mixed effectively, resulting in poor lysis performance. A lysis cell includes a first flexible wall and a second flexible wall, forming a sealed lysis chamber between the first and second flexible walls. The lysis cell also includes an inlet and an outlet, both of which communicate with the lysis chamber. By using the first and second flexible walls, this invention ensures that the liquid entering the lysis chamber through the inlet mixes evenly with the pre-stored reagents within the lysis chamber. Furthermore, the liquid has a longer flow path within the lysis chamber, resulting in more uniform mixing of the liquid and reagents, thereby optimizing the lysis effect of the lysis cell.