51results about How to "Avoid centrifugation" patented technology

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg/ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10% of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg/ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.

The invention discloses a method for extracting and purifying spittle DNA, which comprises the following: 1) a step of pre-treatment, which is to contact the spittle with pretreatment lysis solution, remove solid matters adhered onto cells and obtain coarse lysis solution; 2) a step of nucleic acid absorption, which is to mix the coarse lysis solution obtained by the step 1) with lysis binding solution and magnetic bead suspension to form a solution system containing magnetic nano microsphere and DNA composite and is to collect the magnetic nano microsphere and DNA composite from the solutionsystem; and 3) a step of washing, which is to wash the magnetic nano microsphere and DNA composite obtained by the step 2) with washing liquid I and washing liquid II in turn, and dissolve out DNA byusing eluent. When the method provided by the invention is used, the DNA extraction efficiency may reach 90 percent, the extracted DNA can be used in short tandem repeat (STR) composite amplification, DNA sequencing, DNA quantification and other downstream analysis and operation.

The invention discloses a method for extracting purified DNA from human exfoliative cells. The method comprises the following steps: 1) pretreating: contacting the human exfoliative cells with pretreating lysate to remove the solid matters adhering to the biological cells to obtain coarse lysate; 2) nucleic acid adsorbing: mixing the coarse lysate obtained in the step 1) with lysis integrated liquid and magnetic bead suspension to form a solution system containing a magnetic nano microsphere-DNA compound and collecting the magnetic nano microsphere-DNA compound in the solution system; and 3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step 2) with washing liquid I and washing liquid II respectively and then dissolving out the DNA with eluent. The efficiency of extracting DNA by the method can reach 90% and the extracted DNA can be applied to such downstream analysis operations as STR (short tandem repeat) multiplex amplification, DNA sequencing, DNA quantifying and the like.

The invention belongs to the field of molecular biology and particularly relates to a kit for extracting ribonucleic acid (RNA) by virtue of a paramagnetic particle method and an extraction method. The kit contains tissue digestion fluid, lysate, proteinase K, DNase I, DNase IBuffer, nucleic acid, extraction magnetic beads, washing liquid I, washing liquid II and eluant. The invention further discloses a method for extracting the tissue RNA by virtue of the kit. According to the kit and the method, the extraction yield and purity of RNA are increased, the integrity of RNA is improved, meanwhile, the automatic extraction is realized, and the simultaneous parallel testing of multiple samples is realized, so that the labor cost and the time cost are saved.

The invention discloses a method for extracting and purifying semen DNA. The method comprises the following steps of: (1) pretreatment: keeping semen in contact with a pretreatment lysis solution and removing solid substances adhered to biological cells to obtain a crude lysis solution; (2) nucleic acid adsorption: mixing the crude lysis solution obtained in the step (1) with a lysis binding solution and a magnetic bead suspension solution to form a solution system containing a magnetic nano microsphere-DNA compound, and collecting the magnetic nano microsphere-DNA compound in the solution system; and (3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step (2) by sequentially using a washing solution I and a washing solution II, and dissolving DNA out with eluent. According to the method provided by the invention, the DNA extraction efficiency can reach 90 percent, and the extracted DNA can be applied to STR (Short Tandem Repeat) composite amplification, DNA sequencing, DNA quantification and other downstream analysis operations.

The invention discloses an automatic anti-sedimentation device for a textile assistant. The device comprises a support leg and a stirring motor, wherein a stirring box is fixedly connected with the upper surface of the support leg; a feeding hole and a discharging hole are respectively formed in the surface of the stirring box; a first bearing and a second bearing are respectively fixed on the inner bottom wall and the inner top wall of the stirring box; a supporting plate is fixedly connected with the middle part of the support leg; the stirring motor is fixedly arranged in the middle of the supporting plate; an output shaft of the stirring motor is fixedly connected with a rotating shaft through a coupler. The automatic anti-sedimentation device for the textile assistant can achieve the effect of positively and negatively turning the stirring motor, and meanwhile, the effect of the stirring motor capable of automatically positive and negative turning also can be achieved, the effect of positively and negatively stirring the textile assistant in the stirring box can be achieved, the problem of centrifugal separation of the matters in the textile assistant can be effectively avoided, a contact block connected with the surface of a contact rod can ascend and descend, and an upper travel switch, a lower travel switch, an upper limiting switch and a lower limiting switch can be conveniently touched.

The present invention relates to a process for recovering individual polymers, such as nylon and polyolefin, from a nylon-containing source such as a carpet. The process includes the steps of shredding a nylon fiber source such as a carpet wherein the shredding is conducted in the presence of a liquid that wets the carpet, granulating the wet shredded carpet slurry, further diluting the granulated slurry by adding additional water, subsequently refining the fibers and removing substantial amounts of polypropylene fibers from the slurry, preferably using a lamella clarifier, and recovering the nylon fibers by hydrocloning the slurry.

The invention discloses an amino acid water-soluble fertilizer. The water-soluble fertilizer is prepared from paecilomyces lilacinus fermentation liquor, polyglutamic acid fermentation liquor, micromolecular amino acid, chitosan oligosaccharide, potassium alginate, chelated medium elements and a thickening agent. The product has the advantages that the fermentation liquor is directly used, so thatthe centrifuging or filtering process is avoided, and the cost is reduced; paecilomyces lilacinus is relatively good in nematode control effect, safe and free of pollution. The potash fertilizer increases the toughness of crop roots and can effectively prevent nematode infection; the micromolecular amino acid can be directly absorbed and utilized by the root system to promote rapid growth of thecrop root system or rapid recovery of the damaged root system; chitosan oligosaccharide is matched with organic matters, so that the influence of root-knot nematodes on crops can be reduced, and combined infection of bacteria or fungi on plant root systems is reduced. The microbial agent and the water-soluble fertilizer raw material can be mutually dissolved after being mixed, the solution is in astable colloid state, and conidia of the microbial agent can stably survive and keep biological activity for a long time.

The invention discloses a preparation method of an efficient and stable organic polymer solar cell. A TiO2 nanoparticle crystal layer, an electron transport layer, an organic active layer, a hole transmission layer and a top electrode are sequentially prepared on a transparent conductive electrode to obtain an inverted solar cell structure; and the inverted solar cell structure is subjected to standing treatment in an environment with certain humidity and an oxygen-containing gas. Through the method, the device efficiency can be significantly improved; the improvement amplitude can reach 1.5 times; the stability performance of the device is obviously improved; and the efficiency attenuation amplitude is smaller than 15% after the efficient and stable organic polymer solar cell is exposed in a high-humidity atmospheric environment for 1,000 hours; and the method is simple in technology, low in cost and suitable for large-scale production and application.

The invention discloses a vertical sand making machine. A vertical sand making shell is included. Main bolt holes are formed in the bottom of the vertical sand making shell, and a main hollow structure is arranged at the center in the vertical sand making shell. Two grinding discs are vertically arranged, the preliminary pressure application grinding function of large sand grains between the grinding angles is achieved under the action of gravity of the large sand grains, under the action of a spiral blade, the main function of pressure application grinding again is achieved, and accordingly the grinding efficiency is effectively improved. In addition, the device is provided with an annular array spiral spring compression abutting setting type control mechanism, and under elasticity of a spiral spring, the maximum rotation strength can be controlled so that the maximum extrusion strength can be controlled. In addition, the device is provided with a two-plane rotation sliding friction ball type rotation grinding disc limiting mechanism, the function of limiting of the rotation plane can be achieved, and the centrifuge phenomenon is prevented.

The invention relates to a hepatocyte growth-promoting factor. Due to the adoption of high performance liquid chromatography analysis, the hepatocyte growth-promoting factor has 12 chromatographic component peaks; the number-average molecular weight of the hepatocyte growth-promoting factor is 10,685 daltons, and the weight-average molecular weight is 11,350 daltons; and the hepatocyte growth-promoting factor comprises 18 kinds of amino acid and 10 trace elements. The concentration of the hepatocyte growth-promoting factor is 15.0 to 22.5mg/ml, and the content of active protein in the hepatocyte growth-promoting factor is 71 to 75 percent. In an in-vitro cell 7721 proliferation-promoting test, the proliferation multiple of the hepatocyte growth-promoting factor which is measured by a methyl thiazolyl tetrazoliym (MTT) assay is 4.5 to 6.0. The invention relates to a preparation and application of the hepatocyte growth-promoting factor. The hepatocyte growth-promoting factor has high activity and high active substance content and is safely used.

The invention discloses an assistant precipitation preventing device for textile engineering. The assistant precipitation preventing device comprises a tank body, a base, a supporting platform, a right wheel disk, a toothed plate, an annular gear, a left wheel disk and stirring parts; the outer end surface of the right wheel disk is fixedly provided with a right guide post; the outer circumferenceof the right guide post is slidably sleeved with a right sleeve plate; the outer end surface of the left wheel disk is fixedly provided with a left guide post; the outer circumference the left guidepost is slidably sleeved with a left sleeve plate; each of two ends of the toothed plate is fixedly connected with the right sleeve plate and the left sleeve plate by a push-pull rod; the middle partof an inner cavity of the tank body is rotatably provided with a rotating rod in a bearing connecting way; the outer circumference of the bottom of the rotating rod is fixedly provided with the annular gear; and the outer circumference surface of the rotating rod in the inner cavity of the tank body is symmetrically and fixedly provided with the plurality of stirring parts. The assistant precipitation preventing device is simple in structure, convenient to use and capable of effectively reducing the possibility that stirring dead angles appear and solving the problem that a textile assistant easily generates substance separation under the action of a centrifugal force so as to be suitable for popularization and application.

The invention discloses a green continuous pure-phase Fe5C2 nanoparticle preparation method, which comprises the following steps: preparing an aqueous solution from low-cost ferrite; then adding a reductive aqueous solution to generate black amorphous zero-valent iron precipitates; transferring the black precipitates into an organic high-boiling-point amine solution by use of an external magneticfield; performing reaction for a certain time, transferring the black precipitates into a receiver by use of the external magnetic field, and performing drying to obtain the pure-phase Fe5C2 nanoparticles. According to the method, a new solution and process flow are provided for environment-friendly industrial production of a Fe5C2 nanometer material; the method is simple and easy in process, highin repeatability and suitable for industrial continuous production; the whole reaction system is free of environmental pollution, and an environment-friendly nanopowder synthesis process is implemented; the method is high in practicability, and also has relatively broad application prospect and relatively good economic benefits.

The invention discloses a fungus detection kit. The fungus detection kit comprises magnetic beads and balance liquid, wherein the balance liquid comprises glucose, mannose, galactose and maltose, andthe ratio of the magnetic beads to the balance liquid is (50 mg-100 mg): 1 ml. The invention further discloses a fungus enrichment method. The fungus detection kit is used for enriching fungi quicklyand conveniently, and the cost is low; the enrichment effect is obvious; and repeated centrifugation is avoided.