51results about How to "Avoid centrifugation" patented technology

The invention discloses a method for extracting and purifying DNA, comprising the following steps: 1) pre-treatment: a biological sample is in contact with pre-treated lysis solution, and solid substance adhering to biological cells is removed to obtain rude lysis solution; 2) nucleic acid adsorption: the rude lysis solution obtained in step 1 is mixed with lyse combined liquid and magnetic bead suspension to form a solution system containing magnetic nanosphere-DNA composite body, and the magnetic nanosphere-DNA composite body in the solution system is collected; 3) washing: the magnetic nanosphere-DNA composite body obtained in step 2 is successively washed with a cleaning solution I and a cleaning solution II, and then DNA is dissolved out by spent regenerant. The method provided by the invention has 90% of the DNA extracting efficiency, the extracted DNA can be used in down stream analysis operation, such as STR multiplex amplification, DNA sequencing, DNA quantitation and the like.

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg / ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10% of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg / ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.

The invention discloses a method for extracting and purifying spittle DNA, which comprises the following: 1) a step of pre-treatment, which is to contact the spittle with pretreatment lysis solution, remove solid matters adhered onto cells and obtain coarse lysis solution; 2) a step of nucleic acid absorption, which is to mix the coarse lysis solution obtained by the step 1) with lysis binding solution and magnetic bead suspension to form a solution system containing magnetic nano microsphere and DNA composite and is to collect the magnetic nano microsphere and DNA composite from the solutionsystem; and 3) a step of washing, which is to wash the magnetic nano microsphere and DNA composite obtained by the step 2) with washing liquid I and washing liquid II in turn, and dissolve out DNA byusing eluent. When the method provided by the invention is used, the DNA extraction efficiency may reach 90 percent, the extracted DNA can be used in short tandem repeat (STR) composite amplification, DNA sequencing, DNA quantification and other downstream analysis and operation.

The invention discloses a method for extracting purified DNA from human exfoliative cells. The method comprises the following steps: 1) pretreating: contacting the human exfoliative cells with pretreating lysate to remove the solid matters adhering to the biological cells to obtain coarse lysate; 2) nucleic acid adsorbing: mixing the coarse lysate obtained in the step 1) with lysis integrated liquid and magnetic bead suspension to form a solution system containing a magnetic nano microsphere-DNA compound and collecting the magnetic nano microsphere-DNA compound in the solution system; and 3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step 2) with washing liquid I and washing liquid II respectively and then dissolving out the DNA with eluent. The efficiency of extracting DNA by the method can reach 90% and the extracted DNA can be applied to such downstream analysis operations as STR (short tandem repeat) multiplex amplification, DNA sequencing, DNA quantifying and the like.

The invention belongs to the field of molecular biology and particularly relates to a kit for extracting ribonucleic acid (RNA) by virtue of a paramagnetic particle method and an extraction method. The kit contains tissue digestion fluid, lysate, proteinase K, DNase I, DNase IBuffer, nucleic acid, extraction magnetic beads, washing liquid I, washing liquid II and eluant. The invention further discloses a method for extracting the tissue RNA by virtue of the kit. According to the kit and the method, the extraction yield and purity of RNA are increased, the integrity of RNA is improved, meanwhile, the automatic extraction is realized, and the simultaneous parallel testing of multiple samples is realized, so that the labor cost and the time cost are saved.

The invention discloses a method for extracting and purifying semen DNA. The method comprises the following steps of: (1) pretreatment: keeping semen in contact with a pretreatment lysis solution and removing solid substances adhered to biological cells to obtain a crude lysis solution; (2) nucleic acid adsorption: mixing the crude lysis solution obtained in the step (1) with a lysis binding solution and a magnetic bead suspension solution to form a solution system containing a magnetic nano microsphere-DNA compound, and collecting the magnetic nano microsphere-DNA compound in the solution system; and (3) washing: washing the magnetic nano microsphere-DNA compound obtained in the step (2) by sequentially using a washing solution I and a washing solution II, and dissolving DNA out with eluent. According to the method provided by the invention, the DNA extraction efficiency can reach 90 percent, and the extracted DNA can be applied to STR (Short Tandem Repeat) composite amplification, DNA sequencing, DNA quantification and other downstream analysis operations.

The invention discloses an automatic anti-sedimentation device for a textile assistant. The device comprises a support leg and a stirring motor, wherein a stirring box is fixedly connected with the upper surface of the support leg; a feeding hole and a discharging hole are respectively formed in the surface of the stirring box; a first bearing and a second bearing are respectively fixed on the inner bottom wall and the inner top wall of the stirring box; a supporting plate is fixedly connected with the middle part of the support leg; the stirring motor is fixedly arranged in the middle of the supporting plate; an output shaft of the stirring motor is fixedly connected with a rotating shaft through a coupler. The automatic anti-sedimentation device for the textile assistant can achieve the effect of positively and negatively turning the stirring motor, and meanwhile, the effect of the stirring motor capable of automatically positive and negative turning also can be achieved, the effect of positively and negatively stirring the textile assistant in the stirring box can be achieved, the problem of centrifugal separation of the matters in the textile assistant can be effectively avoided, a contact block connected with the surface of a contact rod can ascend and descend, and an upper travel switch, a lower travel switch, an upper limiting switch and a lower limiting switch can be conveniently touched.

The invention discloses magnetic two-peak mesoporous carbon containing iron-nickel double metals, a preparation method and an application thereof. The magnetic two-peak mesoporous carbon adopts magnetic two-peak mesoporous carbon as a carrier, iron-nickle alloy nano particles are dispersed in the magnetic two-peak mesoporous carbon to form an ordered two-peak mesoporous structure with the hole diameters being 4nm-5nm and 15nm-20nm. The preparation method comprises the steps of preparing a mesoporous silicon template, preparing a compound, preparing the magnetic two-peak mesoporous carbon containing the iron-nickel double metals and the like. The invention adopts an initial-wetting impregnation method to prepare the magnetic two-peak mesoporous carbon containing the iron-nickel double metals; the prepared magnetic two-peak mesoporous carbon containing the iron-nickel double metals is large in specific surface area, uniform in distribution of iron-nickle alloy nano particles, difficult in aggregation, excellent in magnetic performance and stable in physicochemical property, can be applied to removal of dyestuff pollutants in water bodies, and has the advantages of large adsorption capacity, high removing efficiency, wide application range, simplicity and convenience in operation, strong practicability and the like.

The invention discloses a preparation method of uniformly distributed ink for color printing. A blanking hopper is placed on the inner side of a feeding port, hollow supporting blocks are welded to the two ends of the blanking hopper, first vibration springs are welded to the bottom ends of the hollow supporting blocks, one end of the blanking hopper is connected with a first vibration motor, anda fine screen is clamped to the inner side of the blanking hopper, a lifting motor is installed at the top end of a fixed installation frame, a linkage gear is connected with an auger in a welded modethrough a linkage rod, a square scraping plate is placed on the inner side of the blanking hopper, and pigment placed in the blanking hopper is screened and blanked through the vibration springs andthe vibration motor, therefore the pigment is filtered, and meanwhile lifting smashing is conducted through the auger and a lifting supporting plate, and the situation that in the blanking process, large-particle pigment blocks meshes is avoided, pigment particles become small and accordingly are more effectively mixed with a solvent, the pigment is scraped off through a scraper blade, and the situation that due to one-time large-amount blanking, uneven mixing is caused is avoided.

The invention discloses genetically engineered bacterium of co-expressed cyclohexanone monooxygenase and isopropanol dehydrogenase and application thereof. According to the invention, high-level co-expression of two enzymes is realized, enzyme fermentation process is simplified, and production cost is lowered. The genetically engineered bacterium constructed according to the method disclosed by the invention can catalyze and convert high-concentration ufiprazole into esomeprazole, and has an excellent industrial application value.

The invention provides a diethylenetriamine modified magnetic chitosan nanometer particle adsorbent as well as a preparation method and a purpose thereof. The preparation method comprises the following preparation steps of 1, preparing ferroferric oxide magnetic nanometer particles; 2, preparing ferroferric oxide-carbon 18-chitosan-sodium tripolyphosphate polymers; 3, preparing the diethylenetriamine modified magnetic chitosan nanometer particle adsorbent. The material prepared by the method provided by the invention has the advantages that the recovery of the adsorbent is convenient; the complicated centrifugation process is avoided; during the operation, the time is reduced; the operation is simple; the chitosan biological materials achieve the green and environment-friendly effects, are natural, can be degraded, and belongs to sustainable development recycled materials; the diethylenetriamine is used for modifying the chitosan, so that the adsorption site number is increased and the adsorption volume is increased.

The invention discloses a manganese dioxide / corn straw nanomaterial and a preparation method and an application thereof, and belongs to the technical field of nanomaterial preparation. The preparationmethod comprises the steps: peeling corn straw from dried corn stalks, washing cleanly and air-drying, and cutting into 10-20 mg small pieces; taking the corn straw, placing in a potassium permanganate solution, and carrying out a reaction for 60-120 min under continuous stirring, wherein at the moment, the straw changes from white into dark brown; and taking out the straw, washing cleanly and air-drying, to obtain the manganese dioxide / corn straw nanomaterial. The material has the particle size of 3.6-11.7 nm, and the material is attached onto the corn straw. The stirring is completed in anopening environment at room temperature, and no complex instruments and equipment are needed, so the preparation method has the advantages of simpleness, low consumption and environmental protection;the manganese dioxide / corn straw nanomaterial has excellent removal effect on methylene blue dyes, tedious centrifugation or filtering operations during monitoring of purification process and post-treatment are avoided, and the purification process is greatly simplified.

The present invention relates to a process for recovering individual polymers, such as nylon and polyolefin, from a nylon-containing source such as a carpet. The process includes the steps of shredding a nylon fiber source such as a carpet wherein the shredding is conducted in the presence of a liquid that wets the carpet, granulating the wet shredded carpet slurry, further diluting the granulated slurry by adding additional water, subsequently refining the fibers and removing substantial amounts of polypropylene fibers from the slurry, preferably using a lamella clarifier, and recovering the nylon fibers by hydrocloning the slurry.

The invention discloses preparation and immunochromatography application of a magnetic composite nano material with a double-layer quantum dot shell structure. The preparation method of the magnetic composite nano material with the double-layer quantum dot shell structure comprises the following steps: modifying Fe3O4 magnetic nano-particles with polyethyleneimine and adsorbing a layer of carboxylated quantum dots to form a magnetic compound Fe3O4 @QDs with a single-layer quantum dot shell; and then modifying the obtained Fe3O4 @QDs again by using PEI and then adsorbing a second layer of carboxylated quantum dots to form the magnetic composite nano material Fe3O4 @QDs@QDs with the double-layer quantum dot shell structure. The invention also discloses application of the magnetic quantum dottag prepared from the magnetic composite nano material with the double-layer quantum dot shell structure in fluorescence immunochromatography detection. The magnetic quantum dot tag is good in dispersity, high in magnetic responsiveness, adjustable in particle size, high in fluorescence intensity and easy to prepare on a large scale and can be used for capturing, enriching and high-sensitivity detection of target substances in a complex system.

The present invention relates to a process for recovering individual polymers, such as nylon and polyolefin, from a nylon-containing source such as a carpet. The process includes the steps of shredding a nylon fiber source such as a carpet wherein the shredding is conducted in the presence of a liquid that wets the carpet, granulating the wet shredded carpet slurry, further diluting the granulated slurry by adding additional water, subsequently refining the fibers and removing substantial amounts of polypropylene fibers from the slurry, preferably using a lamella clarifier, and recovering the nylon fibers by hydrocloning the slurry.

The invention relates to an automatic filling machine for beverage bottles. The automatic filling machine for the beverage bottles comprises a rack, a conveying chain, a conveying motor, a guardrail,a flushing device, a liquid injection device and a gland device, wherein the liquid injection device comprises a liquid injection rack, a bottle inlet and outlet guide device is arranged on the liquidinjection rack, the bottle inlet and outlet guide device comprises a bottle guide groove, a bottle outlet turntable and a bottle inlet turntable, the liquid injection device further comprises a liquid injection turntable, a liquid injection bottle clamping rotating ring is arranged on the liquid injection turntable, a liquid injection gun is connected to the liquid injection turntable through a liquid injection telescopic supporting rod, the liquid injection gun comprises a vacuum suction pipe connected with the telescopic supporting rod, the upper part of the vacuum suction pipe is providedwith a suction pipe joint, a liquid injection pipe is arranged in the vacuum suction pipe, and the upper part of the liquid injection pipe penetrates through the vacuum suction pipe and is connected to a liquid injection box. According to the automatic filling machine for the beverage bottles, the liquid injection gun is designed to be a structure in which the liquid injection pipe is sleeved withthe vacuum suction pipe, when the liquid injection gun is inserted into bottle bodies, the liquid injection of the beverage bottles in the vacuum state can be realized, meanwhile, the beverage bottles are adsorbed on the liquid injection gun, the centrifugation in the rotating process is avoided, the structural cost of the turntables is saved, and the matching difficulty is reduced.

The invention provides a preparation method of polystyrene microspheres of coupled antibodies. The preparation method comprises the following steps of (1) placing carboxyl polystyrene microspheres ina buffer solution and adjusting the concentration of the carboxyl polystyrene microspheres; (2) adding the antibodies into the buffer solution in step (1) and adding a coupling agent for a coupling reaction; (3) uniformly mixing the solution obtained after the reaction in step (2) and adding a sealing reagent; (4) adding a surfactant into the sealed solution in step (3) and uniformly blending themixed solution to obtain the polystyrene microspheres of the coupled antibodies. According to the provided preparation method, the centrifugation and ultrafiltration processes are omitted, the processdifficulty is lowered, the production cost is reduced at the same time, and the preparation method has a high value in practical application and production.

The invention discloses an amino acid water-soluble fertilizer. The water-soluble fertilizer is prepared from paecilomyces lilacinus fermentation liquor, polyglutamic acid fermentation liquor, micromolecular amino acid, chitosan oligosaccharide, potassium alginate, chelated medium elements and a thickening agent. The product has the advantages that the fermentation liquor is directly used, so thatthe centrifuging or filtering process is avoided, and the cost is reduced; paecilomyces lilacinus is relatively good in nematode control effect, safe and free of pollution. The potash fertilizer increases the toughness of crop roots and can effectively prevent nematode infection; the micromolecular amino acid can be directly absorbed and utilized by the root system to promote rapid growth of thecrop root system or rapid recovery of the damaged root system; chitosan oligosaccharide is matched with organic matters, so that the influence of root-knot nematodes on crops can be reduced, and combined infection of bacteria or fungi on plant root systems is reduced. The microbial agent and the water-soluble fertilizer raw material can be mutually dissolved after being mixed, the solution is in astable colloid state, and conidia of the microbial agent can stably survive and keep biological activity for a long time.

The invention provides a separation and primary culture method for human skin fibroblasts. The method ingeniously integrates respective advantages of an enzyme digestion process and an explant adherence method and avoids centrifugal operation, so an adherence rate is effectively increased while high cell viability is guaranteed, and primary human skin fibroblasts are prepared through simple operation. The invention also provides a method for purifying the human skin fibroblasts and application of purified human skin fibroblasts to establishment of an induced pluripotent stem cell line on the basis of the separation and primary culture method. The methods and application provided by the invention are simple to operate, short in total cell culture time and good in practicality and thus have good application prospects in culture of human skin fibroblasts and establishment of the induced pluripotent stem cell line.

The invention discloses a preparation method of an efficient and stable organic polymer solar cell. A TiO2 nanoparticle crystal layer, an electron transport layer, an organic active layer, a hole transmission layer and a top electrode are sequentially prepared on a transparent conductive electrode to obtain an inverted solar cell structure; and the inverted solar cell structure is subjected to standing treatment in an environment with certain humidity and an oxygen-containing gas. Through the method, the device efficiency can be significantly improved; the improvement amplitude can reach 1.5 times; the stability performance of the device is obviously improved; and the efficiency attenuation amplitude is smaller than 15% after the efficient and stable organic polymer solar cell is exposed in a high-humidity atmospheric environment for 1,000 hours; and the method is simple in technology, low in cost and suitable for large-scale production and application.

The invention relates to a hepatocyte growth-promoting factor. Due to the adoption of high performance liquid chromatography analysis, the hepatocyte growth-promoting factor has 12 chromatographic component peaks; the number-average molecular weight of the hepatocyte growth-promoting factor is 10,685 daltons, and the weight-average molecular weight is 11,350 daltons; and the hepatocyte growth-promoting factor comprises 18 kinds of amino acid and 10 trace elements. The concentration of the hepatocyte growth-promoting factor is 15.0 to 22.5mg / ml, and the content of active protein in the hepatocyte growth-promoting factor is 71 to 75 percent. In an in-vitro cell 7721 proliferation-promoting test, the proliferation multiple of the hepatocyte growth-promoting factor which is measured by a methyl thiazolyl tetrazoliym (MTT) assay is 4.5 to 6.0. The invention relates to a preparation and application of the hepatocyte growth-promoting factor. The hepatocyte growth-promoting factor has high activity and high active substance content and is safely used.

The invention belongs to the technical field of transition metal phosphide composite materials. The invention provides a preparation method and application of a porous molybdenum phosphide / carbon fiber composite material. The preparation method comprises the steps of dissolving a phosphorus source and a molybdenum source into a solution, adding a pore forming agent and a carbon source to prepare aspinning solution, carrying out electrostatic spinning, and carrying out high-temperature carbonization. A dispersed and uniform pore channel structure is generated on the surface of the fiber through shrinkage during pyrolysis of the pore forming agent, and the volume effect caused by ion intercalation and deintercalation in the cyclic process can be effectively relieved, so that the cyclic stability of the material is improved. According to the preparation method, an etching agent and a template agent do not need to be used, a large number of centrifuging and washing processes are avoided,the operation is easy, the morphology is stable and controllable, and continuous preparation is facilitated. The porous molybdenum phosphide / carbon fiber composite material prepared by the invention is used as a negative electrode of a sodium-ion battery, still keeps the specific capacity of 100mAh / g or above after being circulated for 1200 times under the current density of 1A / g, and has good rate capability.

The invention discloses an assistant precipitation preventing device for textile engineering. The assistant precipitation preventing device comprises a tank body, a base, a supporting platform, a right wheel disk, a toothed plate, an annular gear, a left wheel disk and stirring parts; the outer end surface of the right wheel disk is fixedly provided with a right guide post; the outer circumferenceof the right guide post is slidably sleeved with a right sleeve plate; the outer end surface of the left wheel disk is fixedly provided with a left guide post; the outer circumference the left guidepost is slidably sleeved with a left sleeve plate; each of two ends of the toothed plate is fixedly connected with the right sleeve plate and the left sleeve plate by a push-pull rod; the middle partof an inner cavity of the tank body is rotatably provided with a rotating rod in a bearing connecting way; the outer circumference of the bottom of the rotating rod is fixedly provided with the annular gear; and the outer circumference surface of the rotating rod in the inner cavity of the tank body is symmetrically and fixedly provided with the plurality of stirring parts. The assistant precipitation preventing device is simple in structure, convenient to use and capable of effectively reducing the possibility that stirring dead angles appear and solving the problem that a textile assistant easily generates substance separation under the action of a centrifugal force so as to be suitable for popularization and application.

The invention discloses a green continuous pure-phase Fe5C2 nanoparticle preparation method, which comprises the following steps: preparing an aqueous solution from low-cost ferrite; then adding a reductive aqueous solution to generate black amorphous zero-valent iron precipitates; transferring the black precipitates into an organic high-boiling-point amine solution by use of an external magneticfield; performing reaction for a certain time, transferring the black precipitates into a receiver by use of the external magnetic field, and performing drying to obtain the pure-phase Fe5C2 nanoparticles. According to the method, a new solution and process flow are provided for environment-friendly industrial production of a Fe5C2 nanometer material; the method is simple and easy in process, highin repeatability and suitable for industrial continuous production; the whole reaction system is free of environmental pollution, and an environment-friendly nanopowder synthesis process is implemented; the method is high in practicability, and also has relatively broad application prospect and relatively good economic benefits.

The shaft sealing device comprises a motor base, a motor shaft and a coupler, a sealing sleeve is fixedly installed at the bottom end of the motor base, the bottom end of the sealing sleeve is communicated with a kettle body, the bottom end of the motor shaft is fixedly connected with a driving shaft, and the bottom end of the driving shaft is fixedly connected with a bottom plate; a stirring shaft is fixedly connected to the bottom end of the bottom plate, a sealing ring is attached to the surface of the stirring shaft, and a buffer pad is fixedly mounted on the inner wall of the kettle body. According to the scheme, the sealing ring is matched with the oil pipe, so that the problems of poor sealing performance and low flexibility of the stirring shaft when the stirring shaft of the existing ultrahigh pressure reaction kettle is used are solved, lubricating oil enters the oil pipe through the oil guide hole to be used by the stirring shaft, and the sealing performance of the stirring shaft and the inner cavity of the kettle body is enhanced by the sealing ring; and the pressure reducing hole in the stirring shaft reduces the air pressure between the sealing ring and the stirring shaft, so that the dynamic tightness of the stirring shaft is better, and the practicability of the device is enhanced.

The invention discloses a fungus detection kit. The fungus detection kit comprises magnetic beads and balance liquid, wherein the balance liquid comprises glucose, mannose, galactose and maltose, andthe ratio of the magnetic beads to the balance liquid is (50 mg-100 mg): 1 ml. The invention further discloses a fungus enrichment method. The fungus detection kit is used for enriching fungi quicklyand conveniently, and the cost is low; the enrichment effect is obvious; and repeated centrifugation is avoided.

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg / ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10%of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg / ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.