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A kind of hepatocyte growth-stimulating factor, its preparation and application

A technology of hepatocyte growth hormone and preparation, which is applied in the field of hepatocyte growth-promoting hormone to achieve the effect of avoiding the production of antihypertensive substances

Active Publication Date: 2011-12-28
GUANGZHOU YIPINHONG PHARMA +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned patents did not conduct further in-depth research and analysis on the components of hepatocyte growth factor. The inventor has disclosed a component of hepatocyte growth factor in the patent ZL93120999.4, but after the inventor's in-depth research , obtained a new, higher content and more active hepatocyte growth-stimulating hormone

Method used

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  • A kind of hepatocyte growth-stimulating factor, its preparation and application
  • A kind of hepatocyte growth-stimulating factor, its preparation and application
  • A kind of hepatocyte growth-stimulating factor, its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Extraction of hepatocyte growth-stimulating factor

[0059] (1) Material selection: the raw material is fresh suckling pig liver slaughtered on the same day; the weight of a single whole pig liver shall not exceed 140 grams;

[0060] (2) Tissue processing: After the fascia and pedicles of suckling pig liver are removed, wash with pure water; cut into 2-3 pieces with a stainless steel knife to facilitate homogenization;

[0061] (3) Mix with water for injection at room temperature, the speed of homogenate is 800~1000 rpm, the time is 5min, the ratio is water: pig liver mass ratio is 1.1:1; the homogenate is frozen from room temperature within 16°C / min to 0°C, then freeze to -35°C at a rate of 4°C / min;

[0062] (4) After thawing the homogenate that has been frozen at -10~-25℃ for at least 7 days, heat it to 83℃~85℃, remove the heating source immediately, put it into a filter cloth and filter it naturally, and adjust the pH value of the filtrate with NaOH soluti...

Embodiment 2

[0071] (1) Material selection: the raw material is fresh suckling pig liver slaughtered on the same day; the weight of a single whole pig liver shall not exceed 140 grams;

[0072] (2) Tissue processing: After the fascia and pedicles of suckling pig liver are removed, wash with pure water; cut into 2-3 pieces with a stainless steel knife to facilitate homogenization;

[0073] (3) mix with water for injection at room temperature, the speed of homogenate is 800~1000 rpm, the time is 5 minutes, the ratio is water: pig liver mass ratio is 1.15: 1; Freeze at room temperature to -5°C, and then freeze to -35°C at a rate of 5°C / min.

[0074] (4) After thawing the homogenate that has been frozen at -35°C for at least 7 days, heat it to 83°C-85°C and immediately remove the heating source, put it into a filter cloth and filter naturally, and adjust the pH value of the filtrate to 6.5 with NaOH solution; thaw And the heating speed is raised to 0°C at a rate of 2°C / min, and then heated to...

Embodiment 3

[0083] (1) Material selection: the raw material is fresh suckling pig liver slaughtered on the same day; the weight of a single whole pig liver shall not exceed 140 grams;

[0084] (2) Tissue processing: After the fascia and pedicles of suckling pig liver are removed, wash with pure water; cut into 2-3 pieces with a stainless steel knife to facilitate homogenization;

[0085] (3) Mix with water for injection at room temperature, the speed of homogenate is 800-1000 rpm, the time is 5 minutes, the mass ratio of water and pig liver is 1.1:1; the homogenate is frozen from room temperature at a speed of 18°C / min to -2°C, and then frozen to -28°C at a rate of 8°C / min;

[0086] (4) After thawing the homogenate that had been frozen at -30°C for at least 7 days, heat it to 83°C and immediately remove the heating source, put it into a filter cloth and filter it naturally, and adjust the pH value of the filtrate to 6.5 with NaOH solution;

[0087] (5) The solution adjusted to pH 6.5 is ...

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Abstract

The invention relates to a hepatocyte growth-promoting factor. Due to the adoption of high performance liquid chromatography analysis, the hepatocyte growth-promoting factor has 12 chromatographic component peaks; the number-average molecular weight of the hepatocyte growth-promoting factor is 10,685 daltons, and the weight-average molecular weight is 11,350 daltons; and the hepatocyte growth-promoting factor comprises 18 kinds of amino acid and 10 trace elements. The concentration of the hepatocyte growth-promoting factor is 15.0 to 22.5mg / ml, and the content of active protein in the hepatocyte growth-promoting factor is 71 to 75 percent. In an in-vitro cell 7721 proliferation-promoting test, the proliferation multiple of the hepatocyte growth-promoting factor which is measured by a methyl thiazolyl tetrazoliym (MTT) assay is 4.5 to 6.0. The invention relates to a preparation and application of the hepatocyte growth-promoting factor. The hepatocyte growth-promoting factor has high activity and high active substance content and is safely used.

Description

technical field [0001] The invention relates to a hepatocyte growth-stimulating factor, and also relates to its preparation and application. Background technique [0002] Hepatocyte growth-stimulating hormone is a small molecular weight polypeptide mixture extracted from fresh suckling pig liver, with a molecular weight of about 10,000. Its pharmacological effects are mainly to stimulate the DNA synthesis of normal liver cells, promote the regeneration of liver cells, protect the damaged liver cells, promote the recovery of liver function, and also regulate the body's immune function and anti-hepatic fibrosis. It is clinically used to treat severe hepatitis, chronic active hepatitis and liver cirrhosis. [0003] At present, there have been many patents and literature reports on hepatocyte growth-stimulating hormone, a biological agent drug. [0004] In the patent ZL03116052.2, a hepatocyte growth-promoting hormone injection and its preparation method are disclosed, wherein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61P1/16G01N30/02A61K35/407
Inventor 孔祥平陈光明张宜俊杨富强张海松陈阳述
Owner GUANGZHOU YIPINHONG PHARMA
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