12 results about "Pig liver" patented technology

The invention belongs to the technical field of biological medicines, and particularly discloses a pork liver source / photosensitizer-loaded pork liver source lipid nanoparticle and application thereof in endoplasmic reticulum stress enhanced phototherapy. The lipid extraction method provided by the invention has the advantages of mature process, low cost, easily available raw materials, clear components of the obtained lipid, and high biocompatibility. The pork liver source lipid nanoparticles prepared from natural lipid extracted from pork liver have good endoplasmic reticulum targeting property, and can be used as a carrier to efficiently load a photosensitizer IR780; the related nanoparticle preparation method is simple, good in repeatability and easy for expanded production. A phototherapy system constructed by the nanoparticles can generate active oxygen in situ in an endoplasmic reticulum under near-infrared laser irradiation to initiate strong endoplasmic reticulum stress, so that the anti-tumor effect of photothermal and photodynamic combined treatment is remarkably enhanced, and the nanoparticles can be widely applied to efficient and accurate treatment of various solid tumors.

The application discloses a method for detecting clenbuterol based on thin layer chromatography and surface enhanced resonance Raman spectroscopy, and belongs to the technical field of food detection. The method comprises the following steps: (1) adopting thin layer chromatography to preliminarily separate the clenbuterol component from the matrix in the pig liver sample, and confirming the clenbuterol component in the pig liver sample under the ultraviolet lamp of 254 nm; (2) adding aromatic diazonium salt solution to the same Rf value of the clenbuterol component reference sample spot to generate azo compounds, and then adding a surface enhancer silver sol; (3) in-situ detecting the surface enhanced resonance Raman spectrum of the corresponding azo compound component spot, and if the surface enhanced resonance Raman spectrum of the pig liver sample solution at the same Rf value is the same as that of the reference sample solution, it is determined that the pig liver sample contains the clenbuterol component. The application has high specificity for detecting clenbuterol and salbutamol, high sensitivity, fast analysis speed, and is beneficial to fast detection of grass-roots units. The application is suitable for detecting clenbuterol.

The invention discloses a compound enzyme enzymolysis process of a suckling pig liver active peptide as well as a product and application of the suckling pig liver active peptide, and belongs to the technical field of extraction and preparation of functional peptides. The technical problems to be solved are that an adaptive compound enzyme formula is lacked in the existing preparation of the active peptide of the suckling pig liver, the enzymolysis efficiency of a single enzyme or a conventional compound enzyme is low, active peptide components in the suckling pig liver cannot be efficiently released, and the activity is not ideal. In the research and development of bioactive peptides for hepatitis and liver injury, no bioactive peptide from suckling pig liver is efficiently prepared and has a definite liver protection function, and the existing liver protection bioactive peptide has the problems of unstable curative effect and the like. According to the technical scheme, the compound enzyme enzymolysis process is characterized in that compound enzyme is used, and the compound enzyme is composed of neutral protease, papain, trypsin and lipase.

This invention provides a two-phase aqueous extraction apparatus and a method for simultaneously extracting alcohol dehydrogenase and heparin from pig liver, belonging to the field of chemical manufacturing technology. The two-phase aqueous extraction apparatus includes two storage cylinders; each storage cylinder has an independently mounted rotating shaft; a driving wheel is fixedly mounted on each rotating shaft; a first driven wheel is mounted on the left side of the driving wheel; a first horizontal shaft is fixedly mounted at the center of the first driven wheel; the first horizontal shaft is rotatably mounted on the inner wall of the storage cylinder; a first stirring rod is evenly arranged on the outer wall of the first horizontal shaft; a second driven wheel is engaged on the right side of the driving wheel; a second horizontal shaft is fixedly mounted at the center of the second driven wheel. Using this apparatus to extract from pig liver is not only efficient, simple, and environmentally friendly, but also allows for the simultaneous extraction of highly active alcohol dehydrogenase and high-purity heparin. This invention provides new ideas for related fields such as the food and pharmaceutical industries.

The invention relates to the technical field of bioengineering, in particular to application of a pig intestinal exosome miR-320 in regulation and control of lipid deposition of pig livers. The nucleotide sequence of the miR-320 is 5 '-AAAAGCTGGGGTTGAGAGGGCGA-3'. It is found and proved for the first time that by improving the expression level of the miR-320 in pig intestinal exosomes, the miR-320 can act on pig liver cells in a targeted mode and remarkably promote lipid deposition of the pig liver cells, a new key target is provided for molecular breeding, precise nutrition regulation and liver lipid metabolism disease research of pigs, and the application has the advantages that the miR-320 can be applied to the pig intestinal exosomes, and the application of the miR-320 in the pig intestinal exosomes is developed. On the basis, the invention further provides a reinforcing agent containing the miR-320 simulant, the reinforcing agent can be used for promoting liver lipid accumulation, optimizing feed energy distribution and developing related metabolism regulation drugs, and the reinforcing agent has important application value for improving carcass quality and increasing production benefits of live pigs.

This invention discloses the application of the AKR1C1 gene and its encoded protein in breeding pig breeds with superior metabolic function. It belongs to the field of genetic engineering technology, and particularly relates to the application of the AKR1C1 gene in breeding pig breeds with superior metabolic function. The nucleotide sequence of the AKR1C1 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the AKR1C1 gene is shown in SEQ ID NO.2. This invention provides the AKR1C1 gene of the Guangxi Bama miniature pig, clarifies the nucleotide sequence of the AKR1C1 gene and the amino acid sequence of the encoded protein, and provides the application of the AKR1C1 gene in regulating bile acid metabolism in pig liver, providing a new molecular target for pig genetic breeding and contributing to the breeding of pig breeds with superior metabolic function.

The application discloses a kind of multi-fingered dexterous hand self-adaptive conformal control methods for soft slippery pig liver grabbing, to solve the problem of grabbing breakage and slip caused by extremely wet slippery and easily damaged pig liver, the application first constructs and visual guidance through orthogonal coordinate system, establish the preliminary grabbing configuration adapted to the irregular contour of pig liver;Then the dexterous hand is configured as low impedance state, drive finger along the normal compliance closure of pig liver surface, use contact reaction force to induce passive slip and roll of finger palm, realize the geometric conformation from point contact to surface contact;Again, through visual geometric shape closure criterion (fingertip depth across equator and envelope centroid) Trigger smooth variable stiffness locking based on balance point reset, the flexible posture is solidified as rigid grabbing structure in situ, and the dependence on friction is converted into geometric constraint.The application overcomes the risk of low friction slip under the premise of avoiding tissue stress damage, realizes the stable and non-destructive grabbing of soft slippery pig liver.

The present application belongs to the technical field of medical image processing, and provides a double-needle conformal ablation planning method for liver cancer microwave ablation, which comprises the following steps: S1, performing ex vivo pig liver microwave ablation experiment and measuring the axial length of the ablation injury area; using a distance field with weights, obtaining a simulation model of the ablation injury area in the double-needle simultaneous ablation mode with different intervals; S2, scaling the target ablation area along the needle insertion direction; S3, clustering the scaled target ablation area into a plurality of unit blocks, and obtaining the closest paired double-unit blocks; S4, covering the unit blocks with the simulation model to obtain the needle arrangement planning scheme before ablation. The method can solve the problem of rapid conformal ablation needle arrangement of liver tumors in the double-needle ablation mode, and quickly calculate an ablation preoperative planning scheme for conformally covering the target ablation area, thereby providing an optimized preoperative planning reference scheme for liver tumor ablation.

The application discloses a method for synthesizing 2,2',4,5,5'-pentachloro-4-biphenyl alcohol based on a pig liver microsome incubation system, which comprises the following steps: establishing a pig liver microsome incubation system, and adding a substrate 2,2',4,5,5'-pentachlorobiphenyl; the 2,2',4,5,5'-pentachlorobiphenyl is metabolically converted by pig liver microsomes to form 2,2',4,5,5'-pentachloro-4-biphenyl alcohol; after termination of the incubation, isopropyl alcohol, dichloromethane and n-hexane are added to the incubation solution, and after oscillation and centrifugation, the organic phase is transferred and volatilized under high-temperature and reduced-pressure conditions; the 2,2',4,5,5'-pentachloro-4-biphenyl alcohol is directly converted, the lipid impurities are removed through concentrated sulfuric acid sulfonation reaction, the residual 2,2',4,5,5'-pentachlorobiphenyl is separated by using a silica gel column, and thus the purified 2,2',4,5,5'-pentachloro-4-biphenyl alcohol is obtained, and the method has the advantages of simple operation, high conversion efficiency, no interference of isomers, and high purity of target products.

The application discloses a microfluidic pig intestinal tract-vascular-liver multi-organ chip and a construction method and application thereof, and belongs to the cross field of microfluidic chips and organoid technology. The chip performs three-dimensional and dynamic series type co-culture on a pig intestinal tract organoid, pig vascular endothelial cells and a pig liver organoid, and constructs an in-vitro pig liver-intestinal circulation microphysiological system containing a complete 'intestinal tract absorption-blood circulation-liver metabolism' channel. The application can not only highly simulate the microstructure and interactive function of pig intestinal tract and liver tissues, but also can be used for establishing a time-controllable intestinal-liver combined injury and repair model. Compared with a traditional static cell co-culture technology, the application can more simulate in-vivo dynamic physiological and pathological processes, has high specificity and accuracy, and provides a powerful standardized in-vitro platform for the research on the nutrition metabolism of large livestock animals, the evaluation of drugs and the construction of disease models.

The application relates to a pig liver powder preparation method and device based on a step-by-step process, and relates to the technical field of pig liver powder preparation.The application comprises the following steps: collecting the water content of pretreated pig liver; determining an initial thickness value according to the water content; controlling a preset cutting device to cut the pretreated pig liver based on the initial thickness value, and collecting a section detection image of the cut pig liver section; identifying and determining tissue characteristics according to the section detection image; determining a thickness adjustment value according to the tissue characteristics; calculating the sum value between the initial thickness value and the thickness adjustment value and taking the sum value as a cutting thickness value; and controlling the preset cutting device to cut the pretreated pig liver based on the cutting thickness value.The application has the effect of improving the quality of prepared pig liver powder.

The present application relates to the field of bioactive peptides, and particularly relates to a pig liver umami peptide, a preparation method and application thereof. The pig liver umami peptide is selected from at least one of the following polypeptide sequences: ADAW, WDDG, WGD, GDEF and WDH. A pig liver raw material is hydrolyzed by alkaline protease under appropriate enzymatic hydrolysis conditions to obtain a pig liver hydrolysate, the pig liver hydrolysate is filtered by a ceramic membrane, and then is subjected to ultrafiltration by an ultrafiltration membrane with a molecular weight cut-off of 1 kDa, is subjected to Sephadex G-15 gel chromatography separation, and the umami peptide with the lowest binding energy is selected as the pig liver umami peptide. The pig liver umami peptide has significant umami taste, and has strong ACE enzyme inhibitory activity and DPPH free radical scavenging activity. The pig liver umami peptide is applied to preparation of umami base or seasoning, and is applied to preparation of a blood pressure lowering product or an antioxidant active product. The preparation method of the pig liver umami peptide is simple, can effectively reduce the cost, and is easy to popularize.