157 results about "Bone formation" patented technology

The invention discloses an in-situ injectable bone repair adhesive material with a double-sequence osteogenesis promoting function as well as a preparation method and application of the in-situ injectable bone repair adhesive material. The preparation method comprises the following steps: 1) sequentially carrying out dual chemical grafting modification on hyaluronic acid by using methacrylic anhydride and dopamine hydrochloride to obtain a DA-HAMA biomacromolecule with UV curing and adhesion functions; (2) preparing a Ca < 2 + > physical cross-linked sodium alginate microsphere material loaded with an osteogenesis promoting drug by using a micro-fluidic system; and (3) preparing a DA-HAMA solution under dark and heating conditions, adding the vascular promoting factors and the drug-loaded microspheres when the DA-HAMA solution is cooled to room temperature, uniformly mixing, and carrying out centrifugal defoaming and irradiation sterilization to obtain the in-situ injectable bone repair adhesive material. The bone repair adhesive material has the functions of being degradable, injectable, capable of being adhered in a wet state, promoting blood vessels and promoting bone formation, and a brand new strategy is provided for bone defect repair.

The present invention belongs to the field of biomedical engineering technology and relates to a bone organoid, its construction method, and its use. The bone organoid is obtained by three-dimensionally culturing a cell mixture comprising stem cells and macrophages. The bone organoid and its construction method of the present invention enable rapid preparation of bone organoids with minimal differences from natural bone, enabling regulation of cell fate and better bone formation and repair.

The invention discloses a hydrogel / calcined bone porous composite scaffold for a physical auxiliary bone repair material as well as a preparation method and application of the hydrogel / calcined bone porous composite scaffold, and relates to the technical field of biological materials. The preparation method comprises the following steps: combining a calcined bone with a hydrogel material containing free ions through ionic crosslinking, and then preparing a calcium phosphate mineralization layer on the surface of the obtained composite material to obtain the hydrogel / calcined bone porous composite scaffold. The hydrogel / calcined bone porous composite scaffold with good biocompatibility and ultrasonic electric field response prepared by the invention has good osteogenesis ability, does not need to assemble other circuit elements, has good formability, has a function of constructing a dynamic electric field under ultrasonic response, and can be applied to the field of osteogenesis. The method has an extremely wide application prospect in the fields of physical therapy and bone repair.

The invention discloses a glucose responsive composite material as well as a preparation method and application thereof. The composite material sequentially comprises porous metal, a polydopamine layer and a mesoporous silicon dioxide coating from inside to outside, the porous metal is coated with the polydopamine layer, the polydopamine layer is coated with the mesoporous silicon dioxide coating, and preparation raw materials of the mesoporous silicon dioxide coating comprise aminated mesoporous silicon dioxide, aspirin, 4-carboxyphenylboronic acid and sodium alginate. The composite material disclosed by the invention has blood sugar concentration dual-mode response capability, aspirin is suddenly released in a hyperglycemia environment, up-regulation expression of anti-inflammatory factors is remarkably promoted, and an anti-inflammatory effect is dominated; in a normal blood glucose environment, aspirin is slowly released, and the expression of osteogenic factors is remarkably up-regulated, so that osteogenic differentiation is promoted as a leading effect; the dual-mode response realizes accurate regulation and control of ASP dosage, organically combines bone formation promotion and anti-inflammatory functions, and provides a brand new solution for patients with bone defects, especially patients with bone defects caused by diabetes mellitus.

The invention provides a milk protein peptide which takes milk protein as a raw material and has the effects of promoting calcium absorption and improving bone health and application of the milk protein peptide. Specifically, milk protein peptide sequences, including YGGVSL and SQRY, are screened by adopting enzymolysis, sequence identification and molecular docking methods. The milk protein peptide is proved to have high calcium transport promoting capacity in the in-vitro intervention of Caco-2 cells, and the milk protein peptide is proved to have the capacity of promoting osteogenesis and inhibiting bone resorption in the in-vitro intervention of MC3T3-E1 mouse embryo osteoblasts. In animal experiments, by increasing the content of blood calcium and bone calcium, reducing the content of excrement calcium, improving mRNA expression of calcium ion channel protein, calcium binding protein and peptide transporter in intestinal tracts, enhancing the efficiency of calcium entering blood, promoting osteogenesis and inhibiting bone resorption, the milk protein peptide is comprehensively proved to have the capabilities of improving calcium resorption of organisms and improving bone health. The milk protein peptide has both functionality and nutrition, and has a wide application prospect in the aspect of bone health.

The invention relates to the technical field of biomedical materials, in particular to a degradable zinc-based composite GBR film with the surface loaded with a nanometer electrostatic spinning fiber coating and preparation and application of the degradable zinc-based composite GBR film. According to the degradable zinc-based composite GBR membrane with the surface loaded with the nano electrostatic spinning fiber coating, the nano fiber coating is implanted into the surface of a zinc-based material such as zinc alloy by adopting an electrostatic spinning technology, and the degradable zinc-based composite GBR membrane with the surface loaded with the nano electrostatic spinning fiber coating can inhibit excessive release of zinc ions, improve biocompatibility of the zinc alloy and the like, promote osteoblast differentiation and promote angiogenesis. The electrostatic spinning coating on the surface of the zinc-based composite GBR membrane serves as a physical barrier to block invasion of epithelial cells and fibrocytes, and angiogenesis is promoted along with degradation of fibers and slow release of drugs; the porous zinc alloy and the like in the middle layer not only provide mechanical support, but also release zinc ions in the degradation process to achieve multiple functions of resisting bacteria and promoting osteogenesis.

The invention discloses a core-shell structure microneedle patch loaded with tetracycline antibacterial drugs and simvastatin as well as a preparation method and application of the core-shell structure microneedle patch, and belongs to the field of biomedical materials. An antibacterial drug doxycycline is loaded on a shell structure of a substrate layer and a needle body of the microneedle patch, and a core structure contains simvastatin-loaded polydopamine nanoparticles. The microneedle patch can release antibacterial drugs in stages, and the number of bacteria at local parts of a focus can be rapidly reduced in a short time through burst release of the antibacterial drugs in the first stage, so that inflammation spreading is controlled; in the second stage, the antibacterial medicine is slowly released, so that the formation of a new plaque biofilm is inhibited for a long time, meanwhile, simvastatin is slowly released, the effect of locally promoting osteogenesis is achieved, and the synergistic effect of antibiosis and bone regeneration is achieved. The compound has a good application prospect in the treatment of chronic periodontitis, especially intractable periodontitis combined with systemic diseases, such as chronic periodontitis combined with diabetes mellitus.

The invention discloses an application of didymoside in a medicine for promoting postoperative tendon-bone interface fibrous cartilage regeneration. According to the application, an important way for treating the tendon-bone interface injury and preventing the tendon-bone healing disorder is obtained through the research of macrophages on fibrous cartilage tendon-bone interface repair and the influence of didymoside on interface bone formation and structure, fibrous cartilage formation and macrophage polarization regulation, so that the tendon-bone interface injury is further treated.

A bone insert includes a cap having a convex top surface, an elongated stem, and a barrier between the cap and the stem. The stem of the bone insert is inserted into a hole formed in a host bone until the barrier is pressed against the exposed bone. The bone implant can be placed against a small focus contact point on the cap. Liquid cement can be injected into a space volume between the host bone and a bone implant. The cap can be made of a material and / or have surface features that create a strong bond with the cement when the liquid cement cures. The stem can be made of a material and / or have bone ingrowth surface features that create a strong bond with the bone.

This invention discloses a bioactive glass and its preparation method, belonging to the field of bioactive glass technology. It aims to solve the problems of insufficient synergistic effect between antibacterial efficacy and bone-promoting activity in existing materials, as well as the inability to monitor in vivo degradation processes in real time. The bioactive glass comprises 44-48 parts silica; 22-27 parts calcium oxide; 22-27 parts sodium oxide; 5-7 parts phosphorus pentoxide; 2-4 parts europium zinc complex; 10-20 parts chitosan; 1-5 parts lidocaine; and 2-5 parts dilute acetic acid solution. The addition of the europium zinc complex promotes bone formation and enhances antibacterial activity, achieving a highly efficient synergy between anti-infection and tissue regeneration. Simultaneously, it provides optical tracking capabilities, allowing for non-destructive real-time monitoring of the material's degradation behavior and tissue integration in vivo via fluorescence imaging. This invention offers superior material performance and a simple preparation process, providing an innovative solution for the repair of infected bone defects.

The present invention relates to a type A recombinant humanized collagen bone repair material and a preparation method and use thereof. The preparation method of the bone repair material provided by the present invention comprises a first step of precipitation method and a second step of dialysis method. By combining the two-step method of precipitation method and dialysis method, the composition and formation process of natural bone are simulated, and a bone repair material with a high collagen ratio and activity is successfully prepared, and collagen can be gradiently degraded and released, collagen expression activity is increased, and the osteogenesis effect is improved.

The invention provides a bone targeting nano composite carrier, a bone targeting nano composite drug and a preparation method and application of the bone targeting nano composite carrier and the bone targeting nano composite drug, and belongs to the technical field of biological medicine, the bone targeting nano composite carrier comprises epigallocatechin gallate and E7 targeting peptide, and the mass ratio of the epigallocatechin gallate to the E7 targeting peptide is 1: (1.5-2.5); the epigallocatechin gallate and the E7 targeting peptide are connected through a condensation reaction. According to the invention, the bone-targeted nano composite carrier is used for entrapping an osteogenic induction component, and a bone-targeted nano composite drug is obtained through self-assembly. According to the invention, triple functions of osteogenesis promoting drugs, anti-inflammatory / anti-oxidation components and active targeting are integrated, and the bone-targeting nano composite drug not only can directly stimulate bone formation, but also can fundamentally improve the crucial pathological microenvironment in diabetic osteoporosis.

The application discloses a Sr / Li co-doped coating with functions of promoting bone formation and bone immune regulation and a preparation method of the Sr / Li co-doped coating. The preparation method of the Sr / Li co-doped coating comprises the following steps: placing a cathode and a substrate serving as an anode in an electrolyte, performing micro-arc oxidation treatment under stirring, rinsing, drying, and obtaining the Sr / Li co-doped coating on the substrate, wherein the substrate is made of pure titanium or a titanium alloy, and the electrolyte comprises water, a strontium salt, a lithium salt, beta-glycerophosphate sodium and calcium acetate. The Sr / Li co-doped coating is prepared by the micro-arc oxidation method in one step, the Sr / Li co-doped coating has good bone immune regulation activity, thereby effectively promoting the adhesion of preosteoblasts, the proliferation of macrophages and preosteoblasts, the bone differentiation of preosteoblasts and the vascular differentiation of human umbilical vein endothelial cells; and the Sr / Li co-doped coating can in vitro induce RAW264.7 cells to polarize to M2 type by activating the P38-MAPK channel.

A composition and methods of making or use thereof include a plurality of fibers forming a shape for augmenting tendon to bone repair. The physical presence of the plurality of fibers provides initial fixation, while the use of an osteoinductive material provides long term enhancement of bone formation around the site of the tendon to bone repair.

The application belongs to the technical field of biomedical engineering, and relates to a selenium-containing high polymer compound modified zinc alloy material and a preparation method and application thereof. The selenium-containing high polymer compound modified zinc alloy material is obtained by coordination and compounding of a selenium-containing high polymer compound and a zinc alloy, and the selenium-containing high polymer compound is prepared from raw materials including H2N-(CH2)3-Se-(CH2)3-NH2, 1,4-bis(2-hydroxyethyl)piperazine, diisocyanate and polyethylene glycol monomethyl ether through a reaction. The selenium-containing high polymer compound modified zinc alloy material prepared by the preparation method can be used as an oral implant or an orthopedic implant, and can reduce inflammation caused by implantation after being implanted into a body, and realize the synergistic effect of anti-inflammation and bone formation promotion.

The present invention relates to a novel lappaconitine derivative, a preparation method thereof and a pharmaceutical use utilizing its osteogenesis-promoting activity. The lappaconitine derivative induces differentiation of stem cells into osteoblasts, increases bone density if applied to an osteoporosis animal model, and induces osteogenesis in a fracture animal model, and thus can be effectively used to prevent, improve or treat osteoporosis and other bone-related diseases, as well as to treat non-disease fractures caused by physical trauma.

The invention discloses a traditional Chinese medicine ingredient modified bone graft material and a preparation method thereof, and relates to the technical field of bone graft materials. The feed additive is prepared from the following raw materials: calcined bone meal and a traditional Chinese medicine extract, the traditional Chinese medicine extract comprises a radix salviae miltiorrhizae extract and / or a radix scutellariae extract. The preparation method comprises the following steps: preparing calcined bone meal, preparing simulated body fluid and preparing the modified bone graft material. The product material can be applied to a dental bone grafting material. The traditional Chinese medicine extract is loaded on the bone material, and the bone material is modified, so that the bone graft material with good biocompatibility, osteogenesis promoting capability and better osteogenesis induction potential can be obtained.

The invention provides composite hydrogel, photo-thermal response drug release composite hydrogel as well as a preparation method and application of the photo-thermal response drug release composite hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is constructed by taking methylacryloyl gelatin as a matrix and combining lauric acid microspheres carrying an osteogenesis promoting drug baicalin, a reduced graphene oxide photothermal agent and a photoinitiator LAP. Under the irradiation of near-infrared light, the hydrogel can realize local mild temperature rise, induce the lauric acid microspheres to generate solid-liquid phase change, release the entrapped osteogenesis promoting drug as required, realize the synergistic effect of photothermal therapy and controlled release of the drug, and have a good osteogenesis repair effect. The hydrogel has good mechanical properties, biocompatibility and degradability, can be used for local treatment of bone defect parts, and effectively promotes regeneration and repair of oral and maxillofacial bone tissues. The invention provides a novel controllable and efficient treatment thought for bone tissue engineering and regenerative medicine.

The invention is suitable for the technical field of oral implantology, and provides a preparation method and application of a 4D printing stent for simplifying oral bone increment surgery. According to the invention, a 4D printing technology is utilized, polydopamine and sodium alginate are mixed and printed into the hydrogel stent, and after strontium ion crosslinking, the stent shows excellent biodegradability and biocompatibility, can replace a titanium mesh bent stent, effectively maintains osteogenesis space, and reduces high technical sensitivity of an implant bone increment surgery. The stent can accurately adapt to different bone defect outlines, support is provided, healing is accelerated, and the risk of complications is reduced. The device is high in adaptability and easy and convenient to implant, unnecessary tissue stripping and injury can be reduced to the maximum extent, the operation duration is shortened, and postoperative recovery of a patient is facilitated. In a horizontal bone increment operation, the scaffold can provide a firm bone foundation for repair means such as subsequent tooth implantation and the like. A mechanical signal generated by deformation and bioactive components released by the stent have a synergistic effect, and bone tissue regeneration is accelerated.

FIELD: otorhinolaryngology.SUBSTANCE: under endotracheal anesthesia, an endoscope equipped with optics and an instrument for removing pathological contents of the maxillary sinus are inserted into the nasal passage. The inferior nasal concha is displaced medially using a raspatory. Then, stepping back from the edge of the piriform opening by 1.5–2.0 cm in the caudal direction, a mucoperiosteal flap of the lateral wall is separated in the lower nasal passage. The mucoperiosteal flap is displaced posteriorly, exposing the bone skeleton in the projection of the inferomedial angle of the maxillary sinus. Next, using a cold plasma coblator, in ablation mode, the mucous surface of the bone skeleton is evaporated for 2–3 seconds, followed by coagulation of small vessels. Then, a bone valve measuring 10–15 mm is formed from the exposed bone skeleton. The bony valve is retracted in the caudal direction, forming an antrostomy. A 70-degree endoscope is inserted through the formed antrostomy and the pathological contents of the sinus are visualized. In this case, if the cyst is visualized with a coblator in ablation mode, the cyst is evaporated along with its contents, and the remaining cyst is captured and removed. If a polyp is visualized, the polyp stalk is first evaporated, and then the remaining part of the polyp is removed. If a foreign body is visualized, it is removed with forceps. In this case, if the foreign body is encapsulated or located in the thickness of the mucosa, a coblator in ablation mode evaporates part of the mucosa until the foreign body is exposed, and then removed. Next, the sinus cavity is washed with saline solution. The antrostomy is closed with a bone flap. The formed mucoperiosteal flap is placed back in its original place.EFFECT: method allows for endoscopic endonasal removal of pathological contents from the maxillary sinuses with a minimal risk of cyst / polyp recurrence and postoperative complications, reduced blood loss during surgery, shortened surgical time, postoperative rehabilitation periods, and temporary disability.3 cl, 3 ex

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

The application provides a screening method of human amniotic membrane mesenchymal stem cells (hAMSCs) with bone regeneration promoting potential, and uses the protein level of transmembrane protein 119 (TMEM119) as a functional index of the bone regeneration promoting ability of the hAMSCs, and uses an enzyme-linked immunosorbent assay (ELISA) to detect the TMEM119 protein concentration in a lysis solution prepared from 1×10 7 The application uses the TMEM119 protein concentration not lower than 15000 pg / mL as a screening standard of the hAMSCs with bone regeneration promoting potential, and screens the hAMSCs with significant bone regeneration promoting potential from different batches of hAMSCs, so that the hAMSCs meeting the screening standard of the application have obvious bone formation promoting potential, and the technical problem that the hAMSCs with bone regeneration promoting potential are difficult to be accurately identified and screened in clinical transformation is solved.

The utility model discloses a 3D (three-dimensional) printing implant for oral implantation in the technical field of medical equipment, which comprises an implant structure, the implant structure comprises an implant body, the implant body is of a gradient pore net structure, a bioactive layer is arranged on the outer side of the implant body, and the bioactive layer is made of a transparent material. A bone meal attaching and guiding structure is arranged on the inner wall of a mesh hole of the implant body, a surface fixing structure is arranged on the surface of the implant body, and a bottom fixing structure is arranged at the bottom of the implant body. According to the utility model, the implant body is arranged into the net-shaped structure, and the bone meal attachment and guide structure is matched, so that the bone meal can be guided to directionally grow along the net-shaped pores, the traditional osteogenesis waiting stage is skipped, the implant implantation and bone increment are synchronously completed, and the damage to soft tissues caused by repeated bone meal filling and pressing operation is avoided.

The application belongs to the field of treating blood vessel-bone coupling imbalance diseases in traditional Chinese medicine, and particularly relates to a composition for treating blood vessel-bone coupling imbalance diseases, a pharmaceutical preparation and application. Geniposide alone or in combination with crocin I can effectively reduce endothelial cell damage under pathological stimulation conditions, reduce the level of cell apoptosis and the degree of oxidative stress, improve the vascular endothelial function state, and can also significantly inhibit osteoclast differentiation, reduce the number of mature osteoclasts, and reduce bone resorption activity, thereby being conducive to restoring the dynamic balance between bone formation and bone resorption.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The present invention relates to a customized device made of inert and rigid ceramics for rebuilding bone, bridge bone defects or reshaping bone, and more particularly, to the combination of material properties and design to protect fractured portions or volumes to form new bone from movements and external forces that may interfere with bone formation and bone healing. This contributes to the best situation for natural bone healing, thereby improving clinical outcomes. The design of the implant member (1) is based on the initial bone structure (2) and the intended treatment, in which at least a portion of the bone-oriented inner surface (9) of the implant member (1) follows irregularities of the initial bone structure (2) to mechanically support the area around the fixation screws (6) for connecting the implant member to the initial bone structure (2).

To provide bone fortifier dietary supplements with a high osteogenesis promoting effect.SOLUTION: A bone fortifier dietary supplement is characterized by containing as an active ingredient isoflavone, and specific components of plant materials, such as Hordeum vulgare, Ipomoea batatas, Peucedanum japonicum, Pinus, Terminalia, Cocos nucifera, Aristotelia Chilensis, and Brassica oleracea.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of bionic bone scaffolds, and particularly relates to a TPMS structure bionic bone scaffold with an immune regulation function and a preparation method of the TPMS structure bionic bone scaffold. Comprising the following steps: preparing a three-period extremely-small curved surface bionic scaffold and micro-tissues, loading micro-tissue spheres in a methacryloyl gelatin solution, then pouring the micro-tissue spheres into the three-period extremely-small curved surface bionic scaffold, and carrying out photo-crosslinking gel forming to obtain the three-period extremely-small curved surface structure bionic bone scaffold with the immune regulation and control function. The bionic bone scaffold disclosed by the invention can be used for accurately simulating components and structures of natural bones, has good immunoregulation and osteogenesis promoting capabilities, and is expected to cooperate with functionalized micro-tissues to construct an immune microenvironment and promote bone tissue regeneration.

The invention discloses a bone-blood vessel coupling regeneration material based on S1P modified decellularized fish scale bone meal as well as a preparation method and application thereof, and belongs to the technical field of biology. After the fish scales are treated by a decellularization method, sphingosine-1-phosphoric acid (S1P) is efficiently anchored on the surfaces of the fish scales by utilizing a cationic gelatin middle layer, and a novel bone-blood vessel coupling regeneration material is developed. The bone-blood vessel coupling regeneration material retains natural components and topological structures of fish scales, and can guide ordered deposition of calcium and phosphorus to improve bone matrix density and compression strength; the endogenous bone marrow mesenchymal stem cells and vascular endothelial cells are actively collected to migrate to the center of a defect cavity by loading the S1P to simulate the homing principle of natural stem cells in an organism, osteogenesis and angiogenesis signal channels are synchronously activated, and endogenous bone-blood vessel coupling regeneration is rapidly started. The invention provides an effective strategy for regulating and controlling osteogenesis-angiogenesis coupling for the field of bone injury repair.

The application relates to the technical field of biomaterials, in particular to an antibacterial and bone growth promoting composite material and a preparation method and application thereof. An antibacterial and bone growth promoting composite material is provided, which comprises 80-99.5% of metal materials and 0.5-20% of auxiliary materials in terms of weight percentage, wherein the auxiliary materials are at least one selected from strontium-containing compounds, fluorine-containing compounds and zinc-containing compounds. The obtained composite material has strong antibacterial property and bone formation promoting effect, and has the advantages of good biocompatibility, corrosion resistance, metal luster and the like, and can be stably and long-term used as a medical metal material in the orthopedic and dental fields.