30 results about "Osteoblast adhesion" patented technology

Nanotubular structured titanium (Ti) substrates have been coated with nanoparticulate hydroxyapatite (nano-HA). The nano-HA surface is highly adherent to the nanotubular Ti surface and is free of microparticles. The nano-HA coated nanotubular Ti surface promotes osteoblast cell adhesion and is particularly suitable for orthopedic and dental implants where deposition of osteoblasts and other proteins is important in bone formation.

The invention relates to an artificial bone with a porous laminated structure and passages, which belongs to the technical field of biomedical materials. The artificial bone is alternately laminated and formed by compact layers and porous layers which are made of calcium phosphate base biological ceramic materials, and in addition, passages for conveying cells and body fluid are arranged in the direction which forms a set angle with the laminated layers. The preparation method comprises the following step: mixing powder such as hydroxylapatite, beta-tricalcium phosphate and the like through using deionized water as medium to be prepared into pulp. A three-dimensional gel laminated forming system is adopted for preparing ceramic blanks formed by the compact layers, the porous layers and the passages, and then the ceramic blanks are sintered at a high temperature for preparing the artificial bone. The invention has the characteristics that the artificial bone has high mechanical strength, has the porous structure with the effects of osteoplast adhesion, propagation, growth and vascularization, can be used as the artificial bone, can also be used as a bone tissue engineering support frame, and has wide application prospects in clinics in the orthopedics department.

The invention discloses a method for preparing a continuous gentle micro-nano structure on a titanium substrate from the outside to the inside through femtosecond laser processing and then anodizing. The method comprises the three steps of polishing, femtosecond laser processing and anodizing. The continuous gentle micro-nano structure prepared through the method is good in repeatability, high in stability and controllable in size and has excellent mechanical properties and biological properties. Due to the fact that the continuous gentle micro-nano structure is formed by partially converting an implantation material and no obvious separatrix exists between the structure and the substrate, the combination strength of the structure and the titanium substrate is high; in addition, the continuous gentle concave pit structure is more approximate a natural bone structure, and osteoblast adhesion growing is facilitated.

The invention discloses a preparation method for a wettability controllable porous structure of a titanium and titanium alloy surface, and belongs to the technical field of surface modification of biological implant materials. The method comprises the following steps of: performing roughening treatment on the titanium and titanium alloy surface by using a sand-blast, large-grit and acid-etch method (SLA) to prepare a super-hydrophilic surface with a micron-nano double-microcosmic pore structure; preparing a titanium oxide thin film with hydrophilic performance by using a plasma oxidation process, wherein osteoblast adhesion and proliferation experiments show that the surface prepared by the process method has excellent bioactivity; and finally, forming a hydrophobic surface on a rough titanium oxide surface by using a molecular self-assembly method and realizing mutual switching between hydrophilicity and hydrophobicity of the surface by using plasma treatment and ultraviolet irradiation. The hydrophilicity of the surface of an implant is obviously improved in the aspects of stability, durability and the like; and the binding force of the metal implant material and a surface coating can be obviously improved while the bioactivity of the titanium and titanium alloy surface is guaranteed.

The present invention provides a dense-coverage, adherent phosphorous-based coating on the native oxide surface of a material. Disclosed phosphorous-based coatings include phosphate and organo-phosphonate coatings. The present invention also provides further derivatization of the phosphorous-based coatings to yield dense surface coverage of chemically reactive coatings and osteoblast adhesion-promoting and proliferation-promoting coatings on the native oxide surface of a titanium material.

The present invention provides a dense-coverage, adherent phosphorous-based coating on the native oxide surface of a material. Disclosed phosphorous-based coatings include phosphate and organo-phosphonate coatings. The present invention also provides further derivatization of the phosphorous-based coatings to yield dense surface coverage of chemically reactive coatings and osteoblast adhesion-promoting and proliferation-promoting coatings on the native oxide surface of a titanium material.

The invention relates to a bone defect repairing material. The bone defect repairing material comprises a bacteria cellulose membrane, a biologic ceramic material and cellulase, wherein the biologic ceramic material is deposited on a microfiber surface of the bacteria cellulose membrane, and the cellulase is dispersed in the bacteria cellulose membrane and can degrade the bacteria cellulose membrane. When the bone defect repairing material is implanted into bone defect positions, as osteoblasts are attached on the microfiber surface of the bacteria cellulose membrane for growing and the ceramic material is deposited on the microfiber surface of the bacteria cellulose membrane, the osteoblasts growing on the ceramic material deposited on the microfiber surface are wrapped easily, and an intact bone organization is formed by the osteoblasts combining with collagen generated from the osteoblasts. The cellulose membrane is degraded gradually by the cellulase embedded, leaving no residues. Glucose as a degraded product does no harm to cells, and the glucose can support osteocyte copying and growing as nutrients. Furthermore, the invention also relates to a preparation method for the bone defect repairing material and applications of the bone defect repairing material.

The invention provides a preparation method of a degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property. The method comprises the following steps: compounding a degradable medical macromolecular material and an active substance capable of promoting osteoblast adhesion and proliferation, and preparing a film with a three-dimensional structure; carrying out surface functionalization treatment for the film with the three-dimensional structure; carrying out surface modification for the film with the three-dimensional structure after the surface functionalization by adopting the active substance with osteoblast adhesion and proliferation activity; and washing and drying the surface modified film with the three-dimensional structure to obtain a product. Compared with the prior art, the degradable medical macromolecular three-dimensional material for improving the osteoblast adhesion and osteogenic property prepared by virtue of the method can maintain the mechanical property and the structure and realize the combination of the bulk-phase low-concentration long-term expression and surface high-concentration short-time expression of the osteogenic active substance, and the adhesion and three-dimensional growth of the osteoblast can be obviously improved.

The invention discloses a preparation method of a good-biocompatibility, enzyme-responsive and antibacterial titanium material. The preparation method is characterized by comprising the following steps: firstly, constructing a uniform titanium dioxide nanotube on the surface of a titanium material by an anodic oxidation method, and using the uniform titanium dioxide nanotube as an antibiotic-loaded medicine storage reservoir; secondly, modifying hyaluronic acid and chitosan by using dopamine and dihydrocaffeic acid respectively to synthesize catechol-functionalized hyaluronic acid and chitosan, which are named as HA-c and Chi-c and used as a polyelectrolyte anion and a polyelectrolyte cation respectively; constructing a catechol-functionalized polyelectrolyte multilayer film on the surfaceof the antibiotic-loaded titanium dioxide nanotube by using the HA-c and the Chi-c by a layer-by-layer self-assembly technology. By the preparation method, biological coating with dual functions of promoting adhesion and differentiation of osteoblasts and achieving a bacterial responsive antibacterial property is constructed on the surface of the titanium material, and a titanium-based implant isendowed with good antibacterial and osteogenic properties.

A composition for use as a prosthetic biomaterial and associated method. The biomaterial exhibits cytocompatibility, mechanical functionality and osteoblast adhesion between the implant and interfacing surface. The biomaterial is metallic, has a grain size less than about 500 nanometers and has a surface roughness of less than about 800 nm rms.

The method for surface inclusions detection, enhancement of endothelial and osteoblast cells adhesion and proliferation and sterilization of electropolished and magnetoelectropolished Nitinol implantable medical device surfaces uses an aqueous solution of chemical compounds containing halogenous oxyanions as hypochlorite (ClO⁻) and hypobromite (BrO⁻) preferentially 6% sodium hypochlorite (NaClO).

A PHSRN-RGD-containing oligopeptide and a composition for promoting bone formation, which contains such oligopeptide as an effective ingredient. The oligopeptide promotes osteoblastic cell adhesion and differentiation and enhances bone regenerative ability, so that the inventive oligopeptide can be effectively used in regenerative treatment of bone tissue and periodontal tissue.

The invention belongs to the field of titanium alloys, and relates to a 3D printing titanium alloy and a preparation method thereof. The preparation method of the 3D printing titanium alloy comprises the steps: subjecting titanium alloy powder to 3D printing, and then sequentially subjecting an obtained 3D printing titanium alloy body to cleaning and oil removal, sand blasting treatment, high-pressure washing, first-time surface hydrophilic treatment, first-time acid etching treatment, second-time surface hydrophilic treatment and second-time acid etching treatment. By adopting the method provided by the invention, a composite structure of a macroscopic bone trabecula structure and a microcosmic multi-stage micron hole structure can be obtained on the surface of the 3D printing titanium alloy, so that the requirements of osteoblast adhesion and osteogenesis promotion are met, and free powder particles and semi-molten particle residues in a porous layer and a grid structure on the surface of the 3D printing titanium alloy can be effectively removed, and particulate matter and impurity components are prevented from remaining on the surface of the 3D printing titanium alloy.

The invention discloses a biomechanical mandible scaffold and a preparation method of a micro-nano hierarchical permeable titanium-niobium surface. The material of the mandible scaffold has a gradientstructure, and the mandible scaffold comprises an alloy matrix and a porous surface, wherein the alloy matrix is a titanium-niobium-based beta-series alloy material and the porous surface is a micro-nano hierarchical permeable titanium-niobium surface. The biomechanical mandible scaffold has the high-strength and low-modulus alloy matrix and the porous surface, the modulus is further reduced through the porous structure, the morphology beneficial to osteoblast adhesion is formed, and the constructed mandible scaffold better conforms to biomechanical characteristics and has surface osteogenicactivity.

The invention relates to a Cu/porous anodic alumina (PAA) composite membrane as well as a preparation method and application thereof. The composite membrane has a structure that nano-Cu is evenly distributed in a PAA porous structure by means of alternate current (AC) deposition. The preparation method comprises the steps of carrying out barrier layer thinning and pore broadening pretreatment on PAA; adding the PAA subjected to the pretreatment into electro-deposition liquid, and carrying out external AC deposition to obtain the Cu/PAA composite membrane. The method provided by the invention is simple and efficient in operation and convenient to popularize; the prepared composite membrane has antibacterial property and osteoblast adhesion proliferation promoting ability at the same time, thus being expected to come into use as a surface coating of an artificially implanted material.