Preparation method of degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property

A technology of polymer materials and osteoblasts, which is applied in the field of degradable medical polymer material nanocomposite materials and bionic modification, and achieves the effects of strong applicability, good osteogenic performance and low material requirements

Active Publication Date: 2017-09-29
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, so far, there is still no material that combines hybrid modification and surface modification to obtain high expression of o

Method used

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  • Preparation method of degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property

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preparation example Construction

[0025] ginseng figure 1 As shown, the preparation method of a degradable medical polymer three-dimensional material for improving osteoblast adhesion and osteogenic performance in an embodiment of the present invention includes the following steps:

[0026] S1. Combining degradable medical polymer materials with active substances that promote the adhesion and proliferation of osteoblasts to prepare a film with a three-dimensional structure. Specifically, the electrospinning method is used to compound degradable medical polymer materials and active substances that promote the adhesion and proliferation of osteoblasts to prepare a film with a three-dimensional structure. In the film with a three-dimensional structure, the content of the active substances that promote the adhesion and proliferation of osteoblasts is 0.05wt% to 20wt%, and the added active substances that promote the adhesion and proliferation of osteoblasts are evenly distributed without damaging Structural and m...

Embodiment 1

[0033] Weigh 1g PLGA, 10% chitosan, put into trifluoroethanol solution, stir magnetically, dissolve evenly. Under the parameters of positive pressure 18.5kv, negative pressure 2.8kv, humidity 35%, and temperature 19°C, a film with a three-dimensional structure was prepared by electrospinning, and the obtained film was dried in a vacuum oven for 48 hours to remove the solvent; The obtained thin film material with a three-dimensional structure was subjected to surface functionalization treatment with dopamine, in a Tris-HCl solution with a pH value of 8.5 and a concentration of 0.3 wt% dopamine, reacted for 24 h, and washed three times with deionized water. After pretreatment in 2% glutaraldehyde for 1 h, they were washed three times with deionized water. In a 5 wt% collagen solution, react for 24 h, control the temperature at 37 °C, wash with deionized water three times, and dry in a vacuum oven for 48 h to obtain the product.

[0034] The prepared electrospun membrane materia...

Embodiment 2

[0036] Weigh 1 g of PLGA in trifluoroethanol solution, stir magnetically, dissolve evenly, add 250 μL of collagen and mix well with magnetic stirring. A film with a three-dimensional structure was prepared by electrospinning under the parameters of positive pressure 16.23 kv, negative pressure 2.33 kv, humidity 32%, and temperature 19°C. The obtained film was dried in a vacuum oven for 48 h to remove the solvent; The obtained thin film material with a three-dimensional structure was subjected to surface functionalization treatment in a Tris-HCl solution with a pH value of 8.5 and a concentration of 0.1 wt% dopamine, reacted for 24 h, and washed three times with deionized water. In 6 wt% BMP2 solution, react for 36 h, control the temperature at 37 °C, wash with deionized water three times, and dry in a vacuum oven for 48 h to obtain the product.

[0037] The prepared electrospun membrane material has a three-dimensional structure, and the pre-osteoblast MC3T3-E1 culture results...

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Abstract

The invention provides a preparation method of a degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property. The method comprises the following steps: compounding a degradable medical macromolecular material and an active substance capable of promoting osteoblast adhesion and proliferation, and preparing a film with a three-dimensional structure; carrying out surface functionalization treatment for the film with the three-dimensional structure; carrying out surface modification for the film with the three-dimensional structure after the surface functionalization by adopting the active substance with osteoblast adhesion and proliferation activity; and washing and drying the surface modified film with the three-dimensional structure to obtain a product. Compared with the prior art, the degradable medical macromolecular three-dimensional material for improving the osteoblast adhesion and osteogenic property prepared by virtue of the method can maintain the mechanical property and the structure and realize the combination of the bulk-phase low-concentration long-term expression and surface high-concentration short-time expression of the osteogenic active substance, and the adhesion and three-dimensional growth of the osteoblast can be obviously improved.

Description

technical field [0001] The invention relates to the technical fields of degradable medical polymer nanocomposite material and bionic modification, and in particular to a preparation method of a degradable medical polymer three-dimensional material for improving osteoblast adhesion and osteogenic performance. Background technique [0002] Guided bone regeneration (GBR) and guided tissue regeneration have now become a standard approach for optimal soft and hard tissue regeneration in implantology and periodontal treatment. Guided bone tissue regeneration is a widely used oral bone augmentation technique. GBR materials with superior performance are the basis for ensuring the success of GBR surgery. Therefore, research on GBR materials has become an important part of oral tissue engineering. GBR technology mainly places the barrier membrane in the bone defect area, uses the membrane to prevent fast-growing non-osteogenic cells such as epithelial cells from growing into the def...

Claims

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Application Information

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IPC IPC(8): A61L31/06A61L31/04A61L31/02A61L31/14A61L31/16C08G73/06
CPCA61L31/028A61L31/042A61L31/044A61L31/045A61L31/047A61L31/048A61L31/06A61L31/14A61L31/146A61L31/148A61L31/16A61L2300/112A61L2300/232A61L2300/252A61L2300/412A61L2300/414A61L2300/602A61L2400/18C08G73/0672C08L67/04C08L5/08C08L29/04
Inventor 钱蕴珠杨建新孙红
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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