159results about How to "Good osteogenic properties" patented technology

The invention discloses a medical degradable Zn-Cu-X alloy material and a preparation method thereof. The alloy material includes the following components in percentage by weight: 1-10% of Cu, 0-4% of X and the balance Zn, wherein the element X is one of Mg, Ca, Sr, Si, Fe, Mn, Ag, Y, Nd, Gd, Er, Ho and Dy or is a mixture of more of Mg, Ca, Sr, Si, Fe, Mn, Ag, Y, Nd, Gd, Er, Ho and Dy. The invention further relates to the preparation method of the alloy material. The alloy material provided by the invention is a multi-element degradable Zn-based alloy material composed of the human nutrient elements Zn, Cu and X, which completely have biosecurity, has favorable biocompatibility, excellent mechanical properties and an adjustable and controllable corrosion rate, and has a favorable application prospect in the field of medical degradable inner implantation material.

The invention relates to a modification method for a titanium surface with osteogenic performance and antibacterial performance. The modification method adopts a plasma-immersion ion implantation treatment process to co-implant silver ion and calcium ion to the surface of a titanium base material, wherein the co-implantation simultaneously arouse two cathode impulse arc sources, which respectively adopt pure silver and pure calcium as a cathode, to carry out plasma-immersion ion implantation treatment process. The silver-calcium co-implantation method provided by the invention can be used for improving the osteogenic performance and antibacterial performance of the surface (for example an artificial bone, an artificial joint and a dental implant) which can be implanted to medical equipment. The prepared modified material has a remarkable inhibitory effect to the escherichia coli and staphylococcus aureus, and a remarkable promotion to adhesion and proliferation of relevant osteoblast (BMSCs, MG63 cells).

A preparation method of polyvinyl alcohol/hydroxyapatite (PVA/HA) composite microspheres belongs to preparation methods of composite water-in-oil emulsion system microspheres. PVA and HA precursors are used as raw materials to prepare a clear and transparent mixed solution; the clear and transparent mixed solution is dispersed into an oil phase containing a surfactant to form a homogeneous water-in-oil microemulsion; and the PVA/HA composite microspheres can be finally obtained by successive crosslinking reaction under acid-catalyzed conditions, HA deposition under alkaline conditions, product centrifugation, washing, drying and other processes. The method has the advantages of simple process, good repeatability, low energy consumption and low cost, and effectively solves the problems that inorganic nanoparticles are easy to agglomerate in the microspheres to cause uneven structures and poor comprehensive performances and the like. The composite microspheres prepared by the method have the advantages of regular morphology, high dispersibility, uniform microstructure, good biocompatibility, biological activity and adsorption performance, and can be used as drug carrier materials, bone repair materials, environmental water treatment materials, cosmetics or food additives and the like.

The invention relates to a preparation method of multi-ion co-doped hydroxyapatite powder material. The composition formula of the multi-ion co-doped hydroxyapatite powder material is [CaaM10-a][(PO4)6-b-c(SiO4)b(CO3)c][(OH)2-e-fFe(CO3)f], wherein M is metal ions capable of substituting Ca for doping, 0<=a<10, 0<=b<=6, 0<=c<6, 0<=e<2, 0<=f<2, and a, b, c, e and f are not all zero. A hydrothermal preparation method of the multi-ion co-coped hydroxyapatite powder material is simple in operation, high in yield, low in costs of raw materials, applicable to scale production and capable of achieving industrial application prospect and value. The prepared multi-ion co-doped hydroxyapatite powder material is high in osteogenic property, obtains certain antibacterial properties and can be widely applied to the fields such as drug carriers, hard tissue repair materials, food and cosmetics.

The invention relates to a method for preparing a strontium-containing hydroxyapatite coating on the surface of a biodegradable magnesium alloy. The method comprises the following steps: polishing a magnesium alloy matrix to remove a surface oxidation layer, and cleaning; preparing a hydrothermal solution from calcium nitrate tetrahydrate, EDTA-2 Na, sodium dihydrogen phosphate dihydrate and strontium nitrate; and placing the well treated magnesium alloy matrix into the hydrothermal solution for hydrothermal reaction to obtain the strontium-containing hydroxyapatite coating, a biomimetic calcium phosphate coating, wrapping the magnesium alloy matrix. Compared with the prior art, as the hydrothermal method is adopted to prepare hydroxyapatite, not only is the degradation rate of the magnesium alloy in a human body effectively slowed down, but also the biocompatibility of the magnesium alloy is further improved. As strontium element is added into the hydroxyapatite coating, and as one of trace elements of the human body, strontium has the functions of promoting bone formation and inhibiting bone breakage, both the biological activity and the biocompatibility are improved.

The invention relates to the field of medical food and discloses an aquatic collagen peptide food used for adjuvant therapy of osteoporosis and arthritis. Every 100g of the food contains multiple nutritional ingredients as follows: 9-11g of cod skin collagen protein peptide powder, 4-6g of squid skin collagen protein peptide powder, 2-3g of whey protein powder, 2-3g of soybean protein powder, 9-11g of MCT fat, 2-3g of soybean oil, 58-62g of carbohydrate and other vitamins and microelements. The aquatic collagen peptide food takes cod skin collagen protein peptide powder, squid skin collagen protein peptide powder and whey protein powder as main raw materials and has relatively high digestive absorptivity, and collagen protein peptide can promote bone formation and reinforce a bone collagen structure at low calcium level, so that bone strength is improved; bone metabolism can be improved, and ossification can be enhanced; and bone diseases such as the osteoporosis, hyperostosis and swelling and pain of bone joints can be prevented.

The invention relates to a red phosphorus modified titanium implant and a preparation method thereof, and further provides a method for rapidly eliminating a bacterial biofilm on the surface of a boneimplant by utilizing near infrared light. The method comprises the following steps of 1, carrying out surface pretreatment on a titanium sheet; 2, preparing a red phosphorus film on the surface of the titanium sheet so as to obtain Ti-RP; and 3, modifying the surface of the Ti-RP with IR780 and RGDC polypeptide so as to obtain Ti-RP-IR780-RGDC. The method has the advantages that 1, the titanium implant is simultaneously endowed with the surface photo-thermal and photocatalytic properties, so that heat and active oxygen can be generated under near-infrared illumination; and 2, a novel non-surgical and noninvasive treatment method for biofilm infection on the surface of the in-vivo implant is provided, and meanwhile, the implant is endowed with excellent biocompatibility and osteogenic performance.

The invention relates to the technical field of surface coating modification of orthopedic implantation instruments, in particular to a preparation method of a surface coating of an orthopedic implantation instrument, and provides a method for loading and controlling release of copper-citrate nanoclusters on the surface of the orthopedic implantation instrument through polydopamine. The preparation method of the coating is simple and convenient, is wide in application range, and can be applied to various orthopedic implantation instruments. Besides, the change of the ph value can be respondedthrough polydopamine, and the release concentration of the copper ions can be regulated and controlled, so that the conversion between osteogenesis and antibacterial functions of the implanted instrument is controlled.

The invention discloses a CS (calcium silicate)/PEEK (poly(ether-ether-ketone)) composite bone repair material, a bone repair body as well as a preparation method and an application of the material. In the CS/PEEK composite bone repair material, CS accounts for 30%-50% and PEEK accounts for 50%-70%; the particle size of CS is 20-30 mu m, and the particle size of PEEK is 30-40 mum. The CS/PEEK composite bone repair material has excellent mechanical properties, has the elasticity modulus similar to that of natural bone, has better osteogenesis bioactivity, has excellent mechanical compatibility, cannot cause bone resorption due to stress shielding, can promote regeneration of peripheral bone tissue and fusion with natural bone tissue after being implanted into a human body and can meet requirements of immediate fixation and long-term stability of postoperative orthopedic instruments.

The invention provides a preparation method of a degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property. The method comprises the following steps: compounding a degradable medical macromolecular material and an active substance capable of promoting osteoblast adhesion and proliferation, and preparing a film with a three-dimensional structure; carrying out surface functionalization treatment for the film with the three-dimensional structure; carrying out surface modification for the film with the three-dimensional structure after the surface functionalization by adopting the active substance with osteoblast adhesion and proliferation activity; and washing and drying the surface modified film with the three-dimensional structure to obtain a product. Compared with the prior art, the degradable medical macromolecular three-dimensional material for improving the osteoblast adhesion and osteogenic property prepared by virtue of the method can maintain the mechanical property and the structure and realize the combination of the bulk-phase low-concentration long-term expression and surface high-concentration short-time expression of the osteogenic active substance, and the adhesion and three-dimensional growth of the osteoblast can be obviously improved.

The invention relates to a bioactive porous poly-ether-ether-ketone material and a preparation method and application thereof. The active porous poly-ether-ether-ketone material has a pore diameter of450-800 [mu] m and a porosity of 30-95%, and hydroxyapatite microspheres are contained on the inner surfaces of pores. The preparation method comprises the following steps: uniformly adhering the hydroxyapatite microspheres to the surfaces of NaCl particles, performing sintering to obtain composite pore-forming particles, uniformly mixing the composite pore-forming particles with poly-ether-ether-ketone, performing compacting, performing sintering, and finally removing NaCl to obtain the porous poly-ether-ether-ketone material. Osteogenesis promoting medicines and antibacterial medicines canbe loaded into the material according to needs, and the hydroxyapatite microspheres on the inner surface are added, so that the bioinertness of the poly-ether-ether-ketone can be effectively improved,the poly-ether-ether-ketone has bioactivity, the medicines and growth factors can be loaded, a good slow release effect is achieved, and good osteogenesis promoting and antibacterial properties are achieved. The bioactive porous poly-ether-ether-ketone material disclosed by the invention has the advantages of simple process, low cost, controllable structure, convenient drug loading and the like.

The invention discloses an exosome preparation derived from a stem cell. The exosome preparation is an exosome secreted by a Fe3O4 superparamagnetic nanoparticle stimulating the stem cell. The invention further provides the application of the exosome preparation in preparing a pharmaceutic preparation for promoting mesenchymal stem cell osteogenic differentiation and the application of the exosomepreparation in preparing a biological agent for treating orthopedic disorders or bone repair materials. In consideration of the complicated pathophysiologic process of osteonecrosis and bone defects,the exosome preparation prepared by the exosome secreted by the Fe3O4 superparamagnetic nanoparticle stimulating the stem cell is adopted, intramuscular administration can be adopted at local bone defect parts, or local filling is performed after the exosome preparation is mixed with absorbable gel, the exosome preparation directly reaches the focus, the osteogenic activity of the mesenchymal stem cell is motivated, the deposition of osteoblasts is promoted, and the repair of the osteonecrosis or bone defects is promoted.

The invention discloses a preparation method of a biological gradient coating on the surface of a dental implant. The method is used for performing operation of the following steps on the dental implant: polishing a titanium sheet by SiC metallographic abrasive paper, cleaning, drying, and setting the dried titanium sheet on the surface of the dental implant to form a titanium substrate layer; preparing an La-HA/TiO2 composite coating on the surface of the titanium substrate layer; preparing an HA coating on the surface of the La-HA/TiO2 composite coating; and setting the dental implant in an apatite/collagen solution containing La, thus forming the apatite/collagen coating on the surface of the dental implant. According to the invention, the La-HA/TiO2 composite coating is prepared on the titanium surface, so that the excessively high solubility of the HA coating is effectively lowered, the problem of instability between original HA and a metal interface is solved, and the chemical stability and the bonding force of the coating are improved; with the novel coating design, the biological activity and the solubility are improved in a gradient way from inside to outside, and the chemical stability and the bonding strength are improved in a gradient way from outside to inside.

The invention relates to a composite material used for three-dimensional printing of porous tissue scaffold and a preparation method thereof, the material is prepared from, by weight, 50-90 parts of polycaprolactone/polyactic acid/chitosan, 10-50 parts of bioglass/hydroxyapatite/beta-tricalcium phosphate, a composite is mixedly formed, 105-106 cells are added, active factors with suitable concentration are added, and blending printing is carried out during the three-dimensional printing. According to the method, the three-dimensional shape, poriness, a cellular structure can be controlled, biodegradation can be achieved, the mechanical property is effect is better, the mechanical property is better, and the biocompatibility is better.

The invention relates to a novel nano bioceramic coated artificial ligament and a preparation method of the ligament. The novel nano bioceramic coated artificial ligament comprises an artificial ligament and a Ca-Mg-Si bioceramic coating which is uniformly coated on the surface of the artificial ligament, wherein the grain size of ceramic particles of the Ca-Mg-Si bioceramic coating is 20-80nm. By coating the Ca-Mg-Si bioceramic coating on the surface of the artificial ligament, the artificial ligament coated by the coating has multifunctional characteristics of good biological activity, bone formability, bone graft union and the like by means of excellent bone induction capacity of the Ca-Mg-Si bioceramic, so that the artificial ligament becomes a novel graft material with the biological activity.

The invention relates to the technical field of tissue-engineered bones, and especially relates to a construction method and applications of coaxial type vascularized tissue-engineered bone for bone defect repairing. The construction method comprises (1) construction of ossified support in peripheral area; (2) construction of three dimensional blood vessel network in core area; (3) assembly of coaxial type tissue-engineered bones. The constructed coaxial type vascularized tissue-engineered bone can be used as a bone graft for repairing large section weight bearing bone defect, and the repairing effect is good in animal tests. At present, the construction of large volume tissue-engineered bones is still challenging, because the core area of tissue-engineered bone cannot be fully vascularized in the early stage; and the provided construction method can well solve the problem mentioned above so as to construct the vascularized tissue-engineered bone.

The invention provides a bone induction and immunization double-effect coating, a preparation method and application in osseointegration. According to the method provided by the invention, by virtue of a mussel molecule mediated metal-phenol coordination chemical and biological orthogonal reaction strategy, a bone implant with a double-effect coating with immunomodulatory activity and bone induction is designed. Zn <2+> with immunocompetence and BMP-2 peptide with bone induction performance are jointly modified on the surface of the bone implant through mussel molecule surface adhesion chemistry, ion coordination and biological orthogonal reaction, and it is found that the Zn/BMP-2 double-effect coating can better improve the biocompatibility of the bone implant, promote polarization of macrophages to an anti-inflammatory M2 phenotype, and synergistically improve the immune microenvironment of the bone-implant interface to induce the optimal osteogenesis performance and osseointegration effect of the bone-implant interface, so that the in-vivo mechanical stability is improved.

The invention relates to a medical implant of a magnesium alloy/conversion coating and a preparation process of the medical implant. The implant consists of three parts, namely a magnesium alloy AZ31B serving as a matrix, a surface nanocrystallized transition layer and a conversion coating, wherein the conversion coating comprises the following components: Zn3(PO4)2.4H2O, Zn2Mg(PO4)2.4H2O, CaHPO4.2H2O and CePO4.H2O. The process comprises the following steps: forming the nanocrystallized layer on the surface of the magnesium alloy by adopting a multiple high-speed revolution rolling surface nanocrystallization process, and synthesizing the conversion coating on the nanocrystallized surface layer of the magnesium alloy by utilizing a hydrothermal reaction, thereby preparing the medical implant of the magnesium alloy/conversion coating. The implant has high corrosion resistance and has good biocompatibility, and the requirement on clinical application of the implant is met.

The invention discloses construction and application for tissue-engineered bone with BMSCs transfected with genes in a combining manner. According to the invention, recombinant lentiviral vectors carrying human bFGF, BMP-2 and GFP genes are constructed, BMSCs are transfected with the genes, the osteogenesis function of BMSCs can be enhanced through human bFGF and BMP2 gene combined-transfection, the fact that bFGF/BMP2 can adjust OPN, collagen-I and other osteogenesis nonspecific gene expression mechanisms is proved, and a GFP-marked tissue-engineered bone is constructed by gene transfected BMSCs composite heterologous bone scaffold material, and a jaw defect animal model is replanted. According to the invention, defects of single gene transfection are overcome, blood vessel forming and osteogenesis functions for solving difficulties in tissue-engineered bone tissue construction and application thereof to jaw defect repairing are obtained, a bone grafting material which has a wide application prospect is provided for mouth rehabilitation and bone transplantation treatment, and a high practical value and practical significance are realized.

The invention belongs to the field of biomedical engineering, and particularly relates to a method for inducing mesenchymal stem cells to be directionally differentiated into osteoblasts. The method for inducing the mesenchymal stem cells to be directionally differentiated into the osteoblasts has the advantages that the problem of deficiency of a simple, convenient, economical, safe and effectivemethod for inducing mesenchymal stem cells to be differentiated into osteoblasts in the prior art can be solved; macromolecular three-dimensional structures are constructed, appropriate micro-environments are provided, collagen composition and mediation intercellular signal cascade amplification are adopted, culture is carried out on the mesenchymal stem cells in osteogenic induction culture media, accordingly, expression of osteogenesis-related transcription factors can be strengthened, and the mesenchymal stem cells can be promoted to be differentiated into the osteoblasts.

The invention discloses a bone repair material composition, a bone repair material and a preparation method and application of the bone repair material. The bone repair material composition comprisesa bone cement component and curing liquid, wherein the bone cement comprises magnesium oxide, calcium hydrophosphate, sodium dihydrogen phosphate and hydroxyapatite, and the curing liquid is a sodiumphytate aqueous solution. The bone repair material disclosed by the invention is simple to prepare and proper in coagulation time, has good biocompatibility, osteogenesis and degradability, can be directly injected into a bone defect part, has anti-osteoporosis performance and has an obvious osteogenesis effect.

The invention relates to the technical field of surgical implants, and provides double-photoresponse type zinc oxide, a preparation method thereof, and a photosensitive coating with antibacterial osteogenesis. In the preparation process of zinc oxide, sodium citrate and hydroxypropyl methyl cellulose are added to control the morphology, a photothermal conversion material is added to enable zinc oxide to have photothermal conversion capacity, and lignin is added to reduce the energy band gap of zinc oxide; and the freeze-dried hydrothermal product is carbonized through microwave irradiation, and the energy band gap is further reduced, so that the material has response capability to yellow light. The zinc oxide prepared by the invention is of a tremella-shaped wrinkle structure, has dual response to yellow light and near-infrared light, has good adsorbability, antibacterial property and photo-thermal stability, and has photo-thermal conversion capability. The zinc oxide has antibacterialproperty and osteogenesis property, can effectively improve the antibacterial and osteogenesis capacity of the implant when being applied to the surface of the implant, and can realize the light-operated effect and on-demand effect of the antibacterial and osteogenesis functions of the implant due to the special photosensitivity of the coating.

The invention relates to a bone repair material and a preparation method thereof. According to the bone repair material, the effective component consists of calcium sulfate hemihydrate and a Lugua polypeptide injection as raw materials. The method comprises the following steps of: mixing and stirring till the mixed liquid is in a gel shape; then, injecting the mixed liquid to a mould; and after high pressure pressing, taking out and placing at normal temperature to volatilize and cure. The biocompatibility of the bone repair material with human body is good, and has good bone inductive activity, so that bone defect can be quickly repaired, therefore, the bone repair material is very suitable for clinical application of bone surgery.