Construction and application for tissue-engineered bone with BMSCs transfected with genes in combining manner

Abstract

The invention discloses construction and application for tissue-engineered bone with BMSCs transfected with genes in a combining manner. According to the invention, recombinant lentiviral vectors carrying human bFGF, BMP-2 and GFP genes are constructed, BMSCs are transfected with the genes, the osteogenesis function of BMSCs can be enhanced through human bFGF and BMP2 gene combined-transfection, the fact that bFGF / BMP2 can adjust OPN, collagen-I and other osteogenesis nonspecific gene expression mechanisms is proved, and a GFP-marked tissue-engineered bone is constructed by gene transfected BMSCs composite heterologous bone scaffold material, and a jaw defect animal model is replanted. According to the invention, defects of single gene transfection are overcome, blood vessel forming and osteogenesis functions for solving difficulties in tissue-engineered bone tissue construction and application thereof to jaw defect repairing are obtained, a bone grafting material which has a wide application prospect is provided for mouth rehabilitation and bone transplantation treatment, and a high practical value and practical significance are realized.

Description

technical field [0001] The invention belongs to the field of biomedical materials. Specifically, the present invention relates to a technique belonging to the technical field of tissue engineering and biomanufacturing. To be precise, the present invention relates to the technical field of tissue engineering bone tissue construction and its application in the repair of jaw defects. Background technique [0002] Large-area bone defects are more complicated clinically, and require a large amount of bone regeneration, such as bone reconstruction of large-scale bone defects caused by trauma and infection, tumor resection and bone deformity, or under what circumstances, the regeneration process is destroyed, Including avascular necrosis of the femoral head, atrophic fracture and osteoporosis. Currently, the commonly used clinical treatment measures for large-area bone defects are autologous bone transplantation, allogeneic bone transplantation (allogeneic bone and xenograft bone...

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Problems solved by technology

The biocompatibility of the former two has certain problems, and the substances produced by degradation are prone to inflammation and tissue reactions; the latter has relatively good biocompatibility and has a certain osteoinductive ability, but its mechanical properties are relatively poor, so it is not suitable for use. Repair of load-bearing bone defects

[0009] Bone marrow mesenchymal stem cells are a kind of stem cells with multi-directional differentiation potential. BMSCs proliferate stably in vitro and have low decay rate. They have multi-directional differentiation potential in vitro and in vivo. cells, osteoblasts, nerve cells, glial cells, and islet cells, and can secrete a variety of pro-angiogenic and osteogenic factors; however, the expression of angiogenic and osteogenic factors in BMSCs is low To meet the needs of bone tissue repair in defect areas, gene transfection and exogenous addition of growth factors can be used to enhance its angiogenesis and osteogenesis

[0010] At present, there are major research statuses in this field at home and abroad. The problem of repairing large-volume jaw defects lies in the difficulty of vascularization and low osteogenic activity of bone defect repair materials. Clinically, materials and methods for jaw defect repair exist. There are many disadvantages, such as damage to the donor site, poor vascularization function of hydroxyapatite materials, potential immune rejection of heterogeneous bone scaffold materials, and poor treatment effects.

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