1484 results about "Collagen peptide" patented technology

The present invention discloses a natural fruit and vegetable ferment beverage, and a preparation method thereof. The preparation method for the ferment beverage comprises: adding freshly-squeezed fruit and vegetable juice to marine fish skin collagen peptide; adopting the resulting mixture as a fermentation substrate; carrying out dilution, slurry mixing and sterilization, and then adopting a direct vat and freeze-dried type lactic acid starter to carry out fermentation; stopping the fermentation after 7-11 days until the pH value is 4.3; adding a supplementary feed and carrying out sterilization; then adding high-activity dried yeast to carry out fermentation; stopping the fermentation after 5-7 days until the pH value is 4.0; after storing the fermentation broth at the low temperature, carrying out homogenization, concentration, centrifugation, blending, sterilization and bulking to finally prepare the novel natural fruit and vegetable ferment beverage. According to the ferment beverage of the present invention, the stepwise fermentation is adopted for the freshly-squeezed fruit and vegetable juice by using the lactic acid bacteria and the yeast; the marine fish skin collagen peptide is added to promote the growth and the metabolism of fermentation bacteria, such that metabolic products of nutritional ingredients in the natural fruits and vegetables are rich so as to provide a beneficial nutrition and health effect.

The invention discloses a special method to extract collagen from fish peel and bone with complex prolease, which comprises the following steps: choosing salmon, AnKang fish peel and processing waste of fish product as raw material; unfreezing; washing; scaling off; degreasing; grinding; enzymolyzing; separating; de-oiling; ultra filtering; condensing; spray-drying; getting the product. This invention possesses simple craft and short production circle.

The invention discloses an extraction and preparation method for micromolecule fishskin collagen peptide, which comprises the following technical steps of: taking the fishskin of freshwater fish as raw material; removing non-collagenous protein; carrying out steps of degreasing, deodorizing, smashing and the like; under the acidic condition, carrying out heating hydrolysis processing to collagenous protein; controlling the degradation degree of the collagenous protein with an enzymolysis method; obtaining a target micromolecule collagen peptide with a membrane separation method; recycling protease, and then carrying out nanofiltration desalting; and after activated carbon binding resin is decolored and deodorized, carrying out spray drying to obtain the colorless and tasteless micromolecule fishskin collagen peptide. The extraction and preparation method disclosed by the invention has a simple technology, the protease is recycled by membrane separation, and therefore the cost is saved. The prepared micromolecule collagen peptide is colorless and tasteless and can be widely applied to industries relevant to the health of the human body, such as health care food, biomedicine, and cosmetics.

The invention relates to infant formula milk powder and a production method thereof. The infant formula milk powder contains nutrition constituents such as protein, fat, carbohydrate, vitamins, mineral elements and the like with content regulated by the national standards on infant foods. Moreover, whey peptides, collagen peptides and casein phosphopeptides are added in the milk powder. Since the three kinds of binding peptides are added, the invention has the advantages that the immunity of cesarean infants is improved and the disease resistance of the infants is enhanced; the digestive function of the infants is improved, the intestinal mucosa proliferation is promoted and the digestive function is improved; and the development of the nervous system is promoted, the nutrition supply to the nervous system is guaranteed and the memory and the learning ability of the infants are improved.

The invention discloses a health-care food with functions of improving skin moisture and reinforcing immunity. The health-care food comprises the following components in percentage by weight: 1.0 to 80.0 percent of collagen peptide powder, 0.1 to 40.0 percent of glucosamine, 0.1 to 30.0 percent of aloe extract, 0.1 to 50.0 percent of vitamin C (ascorbic acid), and 0.0 to 90.0 percent of auxiliarymaterials. The health-care food can be made into tablets, powder, particles, hard capsules, soft capsules, oral liquids and other oral preparations with various flavors. By virtue of a scientific formula, the raw materials with exact efficacies are compatible reasonably, the collagen peptide powder, the glucosamine, the aloe extract and the vitamin C (ascorbic acid) are selected as main raw materials, and the corresponding auxiliary materials can be selected according to different oral preparations. Furthermore, the selection, purchase, preparation and other aspects of the raw materials are completely operated according to related regulations of health-care foods, so the advancement and stability of the product are ensured; and the health-care food is safe, efficient and stable, and has controllable quality by adopting a modern preparation process matched with a strict quality control method. Toxicological safety tests and function tests prove that the health-care food can be used foreffectively improving the human skin moisture and reinforcing the human body immunity.

The invention discloses a method for preparing collagen peptide from fish scales, which is characterized by comprising the following steps of: 1, taking the fish scales and immersing hydrochloric acid to remove calcium; washing to be neutral, drying and crushing; 2, adding the calcium-removed, dried and crushed fish scales obtained by the step 1 into an enzyme reactor; adding water and mixed protease to extract the collagen peptide; after the extraction, carrying out enzyme deactivation and centrifuging to obtain an enzymolysis solution, wherein the mixed protease is a mixture of neutral protease, papain and flavored protease; and 3, carrying out film concentration on the enzymolysis solution obtained by the step 2; and freezing and drying a concentrated solution to obtain fish scale collagen peptide powder. According to the fish scale collagen peptide obtained by the invention, the neutral protease, the papain and the flavored protease are used for extracting the fish scale collagen peptide by an enzymic method at one step, so that not only can the enzymolysis time (9 hours)be reduced, but also the extracting rate (72.72%) of the collagen peptide is improved, and solves the special flavor problem of bitter taste of the collagen peptide and the like.

The invention relates to a method for preparing a collagen peptide by taking a cod skin as a material; in the method, the cod skin is selected as the material and is washed clearly by fresh water; 0.2 percent-0.5 percent mixed liquid of calcium hyduloxide and sodium hydroxide is added for dipping; then the cod skin is washed by fresh water until the pH value is neutral; after the water liquid of 0.3 percent to 0.6 percent diluted acid is added for dipping, the cod skin is washed by fresh water again until the pH value is neutral; the cod skin is cracked and is stirred and extracted in hot water for extracting a collagen; then undissolved substances are removed through filtering; the pH value of the extraction liquid of the collagen is adjusted to be neutral; under the stirring conditions, enzymes are added for carrying out zymohydrolysis and obtaining a zymohydrolysis liquid; and after the enzymes are eliminated and filtering is carried out, the powder of the collagen peptide is obtained through vacuum condensing and sponging drying. The method has the advantages of simple technique, less device investment, no environment pollution and being suitable for industrialization production. The collagen content of the powder of the prepared collagen peptide is higher; and the color, smell and biological properties are excellent. The powder of the collagen peptide can be broadly applied to the industries of health-care foods, medicines, and the like.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

The invention discloses a process for preparing micro-molecular fish scale collagen peptides and belongs to the technical field of biological extraction. The process comprises the following steps of: 1, pretreating fish scales, and removing impurities and fat on the surfaces of fish scales; 2, extracting fish scale collagen peptides by using a high-temperature sulfuric acid extraction method, wherein the extraction yield is over 95 percent; 3, purifying the extracting solution by adopting the process technology combining centrifugal separation and membrane separation to obtain macro-molecularfish scale collagen peptides of which the purity is over 90 percent; 4, hydrolyzing the macro-molecular fish scale collagen peptides by using protease, and performing enzymic inactivation to obtain the solution of micro-molecular fish scale collagen peptides of which the purity is more than 90 percent and the relative molecular weight is less than 1.5kD; and 5, spray-drying and quickly drying thesolution of collagen peptides to obtain the finished product of micro-molecular fish scale collagen peptides. The micro-molecular fish scale collagen peptides prepared by the process have the advantages of low cost, high yield, high purity and low molecular weight; moreover, in the process, the fish scales discarded in fish processing are fully utilized, and the wastes are changed into the valuables.

The invention relates to an oral nutrition composition for beautifying skin, which contains collagen, hyaluronicacid and vitamin C, wherein the collagen is active small molecule peptide with the molecular weight of 2000-6000Dalton, the hyaluronicacid is sodium salt, zinc salt and kali salt, and the vitamin C is natural or synthesized. The invention overcomes the defects that the collagen can not be absorbed by a human body or hydrolyzed into amino acid because the molecular weight is too big, and most of the collagen loses the beautifying efficacy and the like. Hydroxyproline in collagen peptide is promoted to generate by using the vitamin C through active prolyl hydroxylases, the collagen forms big molecule collagen required by beautifying the skin by crosslinked with the hydroxyproline, the hyaluronicacid is also a better nutrient absorption promoting agent except for special water-holding function and is matched with the vitamin C and the collagen for use, which can achieve more ideal effect of promoting the hyaluronicacid to be absorbed.

The invention discloses a composition containing a haematococcus extract and a collagen peptide. The composition is prepared from the following components in part by weight: 1 to 20 parts of haematococcus pluvialis extract, 1 to 10 parts of collagen peptide and 1 to 90 parts of safflower seed oil. The invention also discloses a preparation method for the composition. Natural astaxanthin in the haematococcus pluvialis extract has the ultrahigh oxidation resistance, ensures the normal metabolism of skin, inhibits the generation of melanin and enhances the effect of collagen; the absorption rate of the collagen peptide is between 95 and 100 percent; the collagen peptide can improve the metabolism of skin cells effectively, and can be utilized by the skin cells more easily, so that the activity of the collagen in the skin is enhanced, and the aims of preserving moisture, resisting wrinkles and nourishing the skin are fulfilled; and due to the addition of the safflower seed oil, the composition contains linoleic acid with high content and vitamin E and can prevent the oxidative deterioration of the astaxanthin, an antioxidant is not needed to be added, and the quality guarantee period can be prolonged by one time.

The invention discloses a method for preparing low-molecular-weight aquatic collagen peptides. Fresh fish scales are delimed with hydrochloric acid, fat and impurities are removed with caustic soda, and then hot water extraction is carried out to obtain a collagen solution, and three kinds of enzymes are used to separate enzyme Decompose collagen, then carry out activated carbon filtration and macroporous resin treatment, collect decolorization liquid, filter through ultrafiltration membrane, freeze-dry, the effective method of preparation molecular weight concentration, high recovery rate, good decolorization effect fish scale low molecular weight collagen, not only can Obtain high-content low-molecular-weight collagen peptides from fish skin and fish scales, remove pigments that affect the appearance of collagen products, and reduce the unique fishy smell of collagen, which can improve the product value of fish collagen products.

The invention relates to a method for processing collagen peptides by using fish leftovers, belonging to the technical fields of aquatic product processing and comprehensive utilization of aquatic product leftovers. In the method, the fish leftovers such as fishskins, scales and fish viscera are used as raw materials for carrying out high-valued development utilization. The method specially comprises the following steps: firstly respectively treating and mixing the raw materials so as to obtain a fish leftover mixture; and then promoting step-by-step enzymolysis with ultrasonic wave, killing enzyme, centrifugalizing, decoloring, filtrating, carrying out reinforcing membrane separation with ultrasonic wave and nano filtrating and freeze drying so as to obtain collagen peptides with different molecular weights. According to the invention, the enzymolysis and membrane separation processes are promoted through ultrasonic wave, enzyme adding amount is less, enzymolysis time is short, production efficiency is high, and the obtained products with different molecular weights has no odor, is light in color, and can be used in industries such as medicaments, health products, food and the like.

The invention discloses a method for preparing collagen peptide rich in hydroxyproline. The method comprises the following steps: firstly, extracting collagen from anglerfish as a raw material by using an acid liquid, subsequently performing salting-out and dialysis purification, hydrolyzing fishskin collagen by using pepsase and flavourzyme under the condition of ultrasonic wave in different steps, removing the fragrance and the color, performing centrifugal separation, and subsequently freeze-drying so as to obtain the collagen peptide rich in hydroxyproline. By adopting the method, waste of anglerfish skin is effectively utilized, and the collagen peptide rich in hydroxyproline is prepared by hydrolyzing anglerfish skin, so that not only is high nutrition value of the collagen peptide extracted from the anglerfish skin utilized, but also the problems that the waste of the anglerfish skin pollutes the environment and the resource is wasted are solved. The method for preparing the collagen peptide rich in hydroxyproline provided by the invention not only is applicable to a fish skin raw material, but also can be applicable to other raw materials, so that the method has a good application prospect.

The invention discloses a preparation method of fish cartilage extracts and an obtained product, comprising the following steps: carrying out alkaline protease enzymolysis on fish cartilage to obtain enzymolysis liquid, carrying out flavorzyme enzymolysis, inactivating enzyme and removing impurities to obtain the fish cartilage extract. The invention overcomes the defects that the existing methodfor producing chondroitine and/ or collagen peptide can not fully utilize the raw materials, the product has low effective components, the raw materials are threatened by pollution or the steps are complex and the like. The invention provides the preparation method of fish cartilage extracts and the obtained product. The invention can acquire lacking products in the market by a simple technical process, further improves use value of the raw materials, not only can solve the problem of lacking collagen peptide in the market and chondroitine, but also change waste material into useful things, reasonably and fully utilize natural resources, make the best use of things and reduce environmental pollution; the invention has the advantages of low raw material cost, high product security and highcontent of effective components.

The invention discloses a compound type biological active peptide product for livestock and poultry, which is formed by mixing the following components in percent by mass: 1%-7% of functional active peptide, 10%-40% of probiotics, 5%-20% of prebiotics, and the balance of auxiliary materials, wherein the functional peptide is one or a combination of several of collagen peptide, glutathione, muramyl dipeptide, soybean protein peptide and casein phosphopeptide. The probiotics refers to one or a combination of several of lactic acid bacteria, saccharomycetes, photosynthetic bacteria, actinomycetes, bacillus and streptococcus faecalis. The prebiotics is one or a combination of several of isomalto-oligosaccharide, fructo-oligosaccharide, galactooligosaccharide and xylo-oligosaccharide. The auxiliary materials refer to one or a combination of several of silkworm chrysalis, fish meal, montmorillonite, diatomite, zeolite powder, corn flour, bran and bean pulp. Additive amount of the product accounts for 0.1%-0.2% of the amount of feed, and the feed can be directly fed after the product is added and uniformly mixed. The compound type biological active peptide product disclosed by the invention belongs to the micro-ecological technical product and is green and environment-friendly.

The method relates to a method for preparing collagen peptide with a fish skin, comprising the steps of: washing, draining and cutting the fish skin into pieces, soaking the fish skin pieces in 75% ethanol for 15 min, then taking them out and washing by soaking them in sterile water for 3-5 times and then draining; putting the fish skin pieces into a fermenter so as to keep the skin pieces with a concentration of 4%-8%, inoculating strains with an amount of 1%-5% and a cell age of 14h-18h for fermentation at a temperature of 30-37 DEG C for 48-56h, with 30%-40% of dissolved oxygen, and after fermentation filtering the fermentation liquor to remove thalluses, then conducting enzyme destruction, vacuum concentration and spray drying to the fermentation liquor, thus obtaining collagen peptide. Compared with present methods for preparing collagen peptide with a fish skin, the method of the invention prepares collagen peptide directly through the fermentation method. Thus the preparation process of collagen peptide is simplified, and the production cost is reduced, without environmental pollution. Therefore, the method of the invention has good application prospect.

The invention relates to a preparation method of giant salamander skin collagen peptide. The method comprises the steps that degreasing and deodorization are conducted simultaneously on a giant salamander skin, black skin and impure protein are removed through alkali liquor, under the condition that the temperature ranges from 20 DEG C to 25 DEG C and the pressure ranges from 150 MPa to 300 MPa, pressure maintaining is conducted for 10-60 min, pH of the giant salamander skin processed through ultrahigh pressure is regulated to 8-10, alkaline protease with the weight of 2%-3% of the skin is added, at the temperature of 45-50 DEG C, continuous stirring and enzymolysis are conducted for 3-6 hours, the mixture is boiled for 1-2 min and cooled to 40 DEG C, pH of the mixture is regulated to 4-5, activated carbon with the mass of 1% of the mixture is added, and stirring and decoloration are conducted; under the condition that the rotating speed is 3000-4500 r/min, centrifugation is conducted on the processed mixture for 10-15 min, microfiltration through a filter membrane with the thickness of 0.45 micron, ultrafiltration through an ultrafiltration membrane with the molecular weight cut-off of 4-5 kD and nanofiltration through a nanofiltration membrane with the molecular weight cut-off of 2-3 kD are conducted on the mixture in sequence to obtain filter liquor, the filter liquor is concentrated to be one half of an original sample, and the concentrated liquor is freeze-dried and smashed to obtain giant salamander skin collagen powder. According to the preparation method of the giant salamander skin collagen peptide, the molecular weight of the collagen peptide is small, and over 90% of the molecular weight is smaller than 1000 Dalton.

The invention discloses a method for grading the molecular weight of anglerfish skin collagen peptide. The method comprises the following steps: soaking anglerfish skin in dilute alkaline liquid; taking out, then washing with water and draining; performing enzymolysis on the anglerfish skin; after deactivating enzyme, adding active carbon for debitterizing and decoloring; entrapping collagen peptide with the molecular weight being more than 10,000 Daltons from the filtrate obtained after the active carbon is filtered out, by a first-level plate type ultrafiltration membrane; entrapping collagen peptide with the molecular weight being more than 2,000 Daltons by a second-level roll type ultrafiltration membrane; and entrapping collagen peptide with the molecular weight being more than 200 Daltons by a roll type ultrafiltration membrane. According to the method, the anglerfish skin is hydrolyzed by using pepsase and flavourzyme step by step during enzymolysis, and the collagen peptide with the molecular weight in broad distribution is subjected to effective molecular weight grading processing after enzymolysis, so the collagen peptide with different molecular weight segments can be obtained and can be effectively utilized.

Stable acidic milk drinks characterized by composed of an acidic milk drink base together with collagen peptides obtained by hydrolyzing collagen and stabilizers. Thus, it becomes possible to add a collagen substance to the existing drinks containing various stabilizers without worsening the stability of the drinks achieved by the stabilizer.

The invention relates to marine collagen peptide beverage and a preparation method thereof. The preparation method comprises the following steps of: solving collagen peptide powder in water, adding beta-cyclodextrin to carry out the fishy smell removing and debitterizing treatment to obtain a solution of collagen peptide; mixing the solution of collagen peptide with grape condensed juice, apple condensed juice, grape seed extract, a sweeting agent, a compound stabilizer, an emulsifier and water according to the proportion, and homogenizing; filling; and sterilizing to obtain the finished product. The beverage comprises the raw materials in percentage by weight: 1-10% of the marine collagen peptide, 2-5% of the grape condensed juice, 2-5% of the apple condensed juice, 0.005-0.05% of the grape seed extract, 0.005-0.05% of the beta-cyclodextrin, 2-5% of the sweeting agent, 0.1-0.5% of the compound stabilizer, and 0.05-0.1% of the emulsifier, and the balance of water. The beverage disclosed by the invention has the advantages of scientific formula, rich nutrition, suitable sweet and sour degree, and fast digestion and absorption. The preparation method has reasonable process, strong operability and short production period and is suitable for the industrial production.

The invention discloses a tortoise shell collagen peptide-containing tortoise herb jelly and a preparation process thereof. The preparation process is simple, short in preparation cycle, high in product stability, easy in quality control. The preparation process comprises the steps of: carrying out enzymolysis on a solid tortoise shell, and degrading collagen into small molecule peptide. Compared with the conventional tortoise herb jelly preparation process, the preparation process provided by the invention has the advantages that peptide molecular weight is small, and a human body can absorb easily so as to play a role of health care; and as the tortoise herb jelly is prepared by using a tortoise shell collagen peptide obtained by enzymolysis, the mouth feel is improved, and the tortoise herb jelly is tender and tasty.

The invention discloses a method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme. The method comprises the steps of deashing and degreasing fish scales used as raw materials; adding complex enzyme mixed by pepsin, papain and trypsin for hydrolyzing, inactivating the enzyme, carrying out coarse filtering, film filtering, vacuum concentrating and freeze drying to obtain the micromolecular collagen peptide. The collagen peptide has the average molecular weight of 500-1,000Da, can be absorbed and utilized by human bodies easily, can be applied to cosmetic, healthcare products, foods, beverages and other industries, and has remarkable economic effect and social effect.