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Preparation method of low molecular weight aquatic collagen peptide

A technology of aquatic collagen and collagen, which is applied in the field of protein peptide extraction, can solve the problems of non-concentrated molecular weight distribution, high amino acid content, and increased production cost, and achieve high whiteness of finished product, high collagen content, and low preparation cost Effect

Inactive Publication Date: 2011-11-30
申铉日
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are currently reports of low-molecular-weight collagen peptides obtained by enzymatically hydrolyzing collagen with multiple enzymes, the low-molecular-weight collagen peptides obtained by enzymatically hydrolyzing collagen with multiple enzymes have poor molecular weight distribution or low-molecular-weight collagen peptides. Excessive phenomenon
As a result, in order to obtain uniform low-molecular components, additional separation equipment such as low-pore ultrafiltration membranes or chromatographic separations will lead to increased production costs and decreased yields.

Method used

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  • Preparation method of low molecular weight aquatic collagen peptide
  • Preparation method of low molecular weight aquatic collagen peptide
  • Preparation method of low molecular weight aquatic collagen peptide

Examples

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Effect test

Embodiment 1

[0030] The invention provides a method for preparing low-molecular-weight aquatic collagen peptide, which is carried out according to the following steps:

[0031] Step 1, deashing fish scales:

[0032] Select 300 g of dried fish scales and wash them, soak and stir them with 30% hydrochloric acid at normal temperature for 15 minutes, deash, then wash repeatedly with distilled water to remove hydrochloric acid, phosphorus and calcium attached to the surface of fish scales, and obtain delimed wet fish scales 258g;

[0033] Step 2, using hot water extraction to prepare water-soluble collagen:

[0034] Take distilled water to repeatedly clean the delimed fish scales, stop cleaning when the pH value of the cleaning solution reaches 6.5; drain the fish scales, put them in 1500ml of sodium hydroxide solution with an equivalent concentration of 0.1N, and use a stirrer at 60 rpm Stir for 12 hours at a high speed to remove fat and miscellaneous proteins from fish scales. At this time,...

Embodiment 2

[0094] The invention provides a method for preparing low-molecular-weight aquatic collagen peptide, which is carried out according to the following steps:

[0095] Step 1, deashing fish scales:

[0096] Select 300g of dried fish scales and wash them, soak and stir them with 4% hydrochloric acid at normal temperature for 50 minutes, deash, then wash repeatedly with distilled water to remove hydrochloric acid, phosphorus and calcium attached to the surface of fish scales, and obtain delimed wet fish scales 258g;

[0097] Step 2, using hot water extraction to prepare water-soluble collagen:

[0098] Take distilled water to repeatedly clean the delimed fish scales, stop cleaning when the pH value of the cleaning solution reaches 6.2; drain the fish scales, put them in 1500ml of sodium hydroxide solution with an equivalent concentration of 0.1N, and use a stirrer at 60 rpm Stir at a high speed for 8 hours to remove the fat and miscellaneous proteins of the fish scales. At this ti...

Embodiment 3

[0113] The invention provides a method for preparing low-molecular-weight aquatic collagen peptide, which is carried out according to the following steps:

[0114] Step 1, deashing fish scales:

[0115] Select 300 g of dried fish scales and wash them, soak and stir them with 35% hydrochloric acid at normal temperature for 10 minutes, deash, then wash repeatedly with distilled water to remove hydrochloric acid, phosphorus and calcium attached to the surface of fish scales, and obtain delimed wet fish scales 258g;

[0116] Step 2, using hot water extraction to prepare water-soluble collagen:

[0117] Take distilled water to repeatedly clean the delimed fish scales, and stop cleaning when the pH value of the cleaning solution reaches 7.0; drain the fish scales, put them in 1500ml of sodium hydroxide solution with an equivalent concentration of 0.1N, and use a stirrer at 60 rpm Stir at a high speed for 24 hours to remove the fat and miscellaneous proteins of the fish scales. At ...

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Abstract

The invention discloses a method for preparing low-molecular-weight aquatic collagen peptides. Fresh fish scales are delimed with hydrochloric acid, fat and impurities are removed with caustic soda, and then hot water extraction is carried out to obtain a collagen solution, and three kinds of enzymes are used to separate enzyme Decompose collagen, then carry out activated carbon filtration and macroporous resin treatment, collect decolorization liquid, filter through ultrafiltration membrane, freeze-dry, the effective method of preparation molecular weight concentration, high recovery rate, good decolorization effect fish scale low molecular weight collagen, not only can Obtain high-content low-molecular-weight collagen peptides from fish skin and fish scales, remove pigments that affect the appearance of collagen products, and reduce the unique fishy smell of collagen, which can improve the product value of fish collagen products.

Description

technical field [0001] The invention relates to the technical field of protein peptide extraction, in particular to a method for preparing low-molecular-weight aquatic collagen peptides. Background technique [0002] Collagen is the protein with the most content and widest distribution in the human body. It is the main protein component of connective tissue, accounting for about 25% of the total body protein. It is mainly distributed in animal skin, bone, cartilage, cornea, inner wall of blood vessels and other tissues . Collagen extracted from animal connective tissue is widely used in industries related to human health, such as food, cosmetics and medical treatment, due to its special protein structure and body affinity. At present, the collagen used in industrial production is mainly type I collagen, which is a high-molecular hard protein composed of three 1000 amino acid peptide chains. It is difficult for people to digest high-molecular collagen directly, and the colla...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/36C07K1/34C07K1/16
Inventor 申铉日
Owner 申铉日
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