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Method for preparing collagen peptide rich in hydroxyproline

A technology of hydroxyproline-rich collagen and collagen, which is applied in the direction of fermentation, etc., can solve the problems of ineffective use of anglerfish skin waste, lack of preparation of hydroxyproline-rich collagen peptides, etc., and achieve good application prospects and high Effects of nutritional and medicinal value

Active Publication Date: 2014-03-12
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the existing waste of anglerfish skin has not been effectively utilized, and at the same time solve the problem of the lack of methods for preparing hydroxyproline-rich collagen peptides in the prior art, and provides a method for preparing hydroxyproline-rich collagen peptides Methods

Method used

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  • Method for preparing collagen peptide rich in hydroxyproline

Examples

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Effect test

Embodiment 1

[0034] Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 4.5% NaOH solution to the anglerfish skin fragments, soak for 24 h, take out, and then wash with water until the pH is neutral. Add 35 L of 9% n-butanol solution to the fish skin, stir for 28 h, take it out, wash with water and drain. The drained anglerfish skin fragments were mashed, added 60L of 4% citric acid solution, heated to 55°C, and extracted with constant stirring for 6 h, and the crude collagen extract was obtained after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.5h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 30Hz, and the electric field intensity as 25kV / cm. After taking it out, add 0.8 mol / L NaCl solution to the crude collagen extract for salting out for 20 hours, and then...

Embodiment 2

[0036]Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 2.5% NaOH solution to the anglerfish skin fragments, soak them for 30 h, take them out, and then wash them with water until the pH is neutral. Add 35 L of 9% n-butanol solution to the fish skin, stir for 20 h, take it out, wash with water and drain. The drained anglerfish skin fragments were mashed, added 60 L of 1% acetic acid solution, heated to 65 °C, and extracted with constant stirring for 7 h, and the crude collagen extract was obtained after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.5h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 40Hz, and the electric field intensity as 15kV / cm. After taking it out, add 0.9 mol / L NaCl solution to the crude collagen extract for salting out for 24...

Embodiment 3

[0038] Take 1000 g of washed anglerfish skin and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 25L of 5.5% NaOH solution to the anglerfish skin fragments, soak them for 248 h, take them out, and then wash them with water until the pH is neutral. Add 35 L of 9% n-butanol solution to the fish skin, stir for 25 h, take it out, wash with water and drain. The drained anglerfish skin fragments were mashed, and 60L of citric acid solution with a mass fraction of 2% was added, heated to 90°C, and extracted with constant stirring for 5 h, and the crude collagen extract was obtained after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.5h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 35Hz, and the electric field intensity as 20kV / cm. After taking it out, add 1mol / L NaCl solution to the crude collagen e...

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Abstract

The invention discloses a method for preparing collagen peptide rich in hydroxyproline. The method comprises the following steps: firstly, extracting collagen from anglerfish as a raw material by using an acid liquid, subsequently performing salting-out and dialysis purification, hydrolyzing fishskin collagen by using pepsase and flavourzyme under the condition of ultrasonic wave in different steps, removing the fragrance and the color, performing centrifugal separation, and subsequently freeze-drying so as to obtain the collagen peptide rich in hydroxyproline. By adopting the method, waste of anglerfish skin is effectively utilized, and the collagen peptide rich in hydroxyproline is prepared by hydrolyzing anglerfish skin, so that not only is high nutrition value of the collagen peptide extracted from the anglerfish skin utilized, but also the problems that the waste of the anglerfish skin pollutes the environment and the resource is wasted are solved. The method for preparing the collagen peptide rich in hydroxyproline provided by the invention not only is applicable to a fish skin raw material, but also can be applicable to other raw materials, so that the method has a good application prospect.

Description

technical field [0001] The invention relates to the field of fish collagen peptide preparation, in particular to a method for preparing hydroxyproline-rich collagen peptide. Background technique [0002] Anglerfish is a cold-temperate bottom fish widely distributed in the eastern waters of my country (the northern part of the East China Sea, the Yellow Sea and the Bohai Sea). In recent years, with the continuous increase in the processing of anglerfish in my country, a large amount of leftovers such as fish skin are left in the processing process, which pollutes the environment and causes a waste of resources. Relevant personnel have done relevant research on chondroitin sulfate, dermatan sulfate, collagen and other active substances in the by-products of anglerfish processing. It has been found that the preparation of collagen peptides by hydrolyzing the collagen of anglerfish can significantly improve its nutritional value and digestion. Its collagen peptide can maxi...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 杨会成李八方郑斌马华威
Owner ZHEJIANG MARINE DEV RES INST
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