59 results about "China sea" patented technology

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.

The invention discloses a comprehensive hydrate simulation system and an experimental method thereof. Generally, the simulation system includes a negative pressure sample chamber for placing a sample,and a confining pressure system connected to the negative pressure sample chamber, a parameter measurement system, a constant temperature system, a vacuum system, a gas injection system, a liquid injection system, a heat injection system, a drilling fluid circulation system, a return pressure system, and a data acquisition processing unit. The simulation system of the invention can simulate exploration of natural gas hydrates in all aspects, can be applied in indoor research and synthesis of natural gas hydrates in China sea areas and decomposition mechanisms of natural gas hydrates, and canprovide support for physical simulation of reservoir physical properties, temperature, pressure, laws of yield changes and other important sensitive parameters affecting trial exploration of sea hydrates during the trial exploration processes in different development modes and different development well group conditions.

The invention relates to a method for performing inversion on the optical property of aerosol of a coastal zone, and provides a new algorithm for performing inversion on the optical property of the aerosol of a coastal area in China by using a satellite image aiming at the characteristics of a large number of aerosol particles with high reflectivity and strong absorbability on the ocean surface of the coastal area in China. In the algorithm, the research is conducted by taking an image of a moderate resolution imaging spectroradiometer (MODIS) as an example; the inversion of the optical property of atmospheric aerosol over a muddy water area is finished by using the images of long wave bands of 1,240 nm, 1,640nm and 2,133 nm of the MODIS and a lookup table; and the correctness and applicability of the algorithm are verified by using an foundation observation result of a cleaning day. Theoretical basis is provided for adding a channel with long wave bands and transmissivity into a Chinese sea color remote sensor, the condition of the aerosol over the coastal zone can be reflected in real time, and decision basis is provided for related functional departments.

The invention relates to an aquaculture method of deep sea scallops, in particular to a breeding and propagation method for Atlantic deep sea scallops. The method comprises the steps that introduction, artificial breeding and aquaculture of the deep sea scallops are carried out, parent scallops are mature after 45-50 days maturity promoting and lay eggs, and after most larvae grow into D-shaped larvae, a bolting cloth net cage is used for selecting optimal larvae to a new pond for cultivation; the growth rate of the cultured larvae is far higher than that of the larvae cultured in a nursery ground in Quebec of Canada at the same time, eyespots are generated on most larvae 16-17 days after the larvae are fertilized, adhering substances are arranged at the 18th day, after the adhering substances are arranged for 10 days, the adhering substances are taken out and hung in natural sea ears for larva protection; the deep sea scallops meet commercial specification when the cultivated average specification reaches 8 centimeters, and the deep sea scallops achieve sexual maturity. According to the method, the Atlantic deep sea scallops are introduced into China sea areas for the first time, the scallop culture species are expanded, and construction of modern ocean ranches is promoted.

The invention relates to a hybrid breeding method for stichopus japonicus, comprising the following steps: selecting stichopus japonicus in China sea areas and in Korea sea areas as parental stichopus japonicus, respectively controlling breeding water temperature, times and time of feeding of the parental stichopus japonicus in an artificial culture, and adding yolk and pachyrhizus powder of different amounts for stimulating gonadogenesis in breeding bait containing alga powder, compound feed and holothurian vitamin to enable synchronous maturity of gonads of the parental stichopus japonicus; stimulating the parental stichopus japonicus by a shade-drying running water stimulating method, a temperature differential method and an artificial induced spawning method combined with a method of using spermia to stimulate ovum, to induce the oviposition and the spermiation of the parental stichopus japonicus; and respectively collecting the ovum and the spermia of the parental stichopus japonicus; carrying out reciprocal hybridizations to produce oosperm; and breeding according to a conventional method. The stichopus japonicus sprout cultured by the method has high survival rate, fast growth rate, good stress resistance, and strong ability of resisting mechanical damage. The process is easy and convenient, and can be operated easily, effectively shortens the period of breeding the stichopus japonicus and saves the breeding cost.

The present invention relates to seawater aquiculture technology, and is especially pond cultivation technology of East China sea lefteye mandarin. The pond cultivation technology includes adopting wild East China sea lefteye mandarin as parent fishes; promoting mature and induced spawning of parent fishes through controlling lighting, water temperature and reinforced nutrients; and sea water aquiculture, during which underground sea water is utilized in the high temperature period. The present invention has high survival rate of fry and young East China sea lefteye mandarin as one new aquiculture variety.

The invention relates to a method for calculating high-precision Chinese offshore sea wave characteristic distribution. According to the method, a meteorological model WRF, a large-area sea wave modelwavewatch 3 and a sea wave model swan used in the offshore area of China are combined, and a multi-year historical sea wave characteristic data product with the spatial resolution of 0.04 degree * 0.04 degree can be calculated. As the China offshore area is divided into the East China sea area and the South China sea area by considering the monsoon influence area and the wave propagation characteristics during model establishment. The western pacific ocean is prevented from being brought into a mode range, and computing resources are prevented from being consumed. Due to the fact that the twosea areas have the overlapping area, the overlapping area has two calculation results, a weighted average method is used, the calculation results of the two overlapping areas are integrated, and thewhole set of data has spatial continuity.

The invention discloses an ecological risk assessment method of a polycyclic aromatic hydrocarbon compound and a mixture thereof in a marine water environment. The assessment method comprises the following steps of determining the type and concentration of the polycyclic aromatic hydrocarbon in the marine water environment; screening the representative biological species having the flora characteristics of the creatures of the China sea area; acquiring the chronic toxicity data of polycyclic aromatic hydrocarbon in the water body of a target sea area; using a statistical extrapolation method to calculate the predicted no effect concentration of polycyclic aromatic hydrocarbon; using a joint probability curve method to calculate the level of the ecological risk of single polycyclic aromatic hydrocarbon in the water body of the target sea area; using a toxicity equivalent factor method to calculate the joint ecological risk of the polycyclic aromatic hydrocarbon mixture in the water body of the target sea area; identifying the risk of the monomer and the mixture of the polycyclic aromatic hydrocarbon according to the above results. The ecological risk assessment method of the polycyclic aromatic hydrocarbon compound and the mixture thereof in the marine water environment provided by the invention realizes the ecological risk assessment of the polycyclic aromatic hydrocarbon monomer and the mixture thereof in the China marine water environment by using the toxicity equivalent and the probability risk assessment method based on the species sensitivity distribution theory, and provides a decision basis for the marine ecological risk management of the China organic pollutants.

The invention provides a method for extracting and purifying pepsin-soluble collagens of black sea cucumbers in East China Sea. The method comprises the following steps: soaking a clean, massive black sea cucumber sample in East China Sea with an EDTA (Ethylene Diamine Tetraacetic Acid) solution; performing homogenized homogeneity; performing extraction in sequence by using a Tris-HCl solution, a NaOH solution and a pepsase acetic acid solution; and performing salting out and dialysis to obtain the pepsin-soluble collagens. According to the invention, the EDTA chelating agent is used to remove chemical element with high pollution concentration from sea cucumber tissues, and the removal efficiency can reach more than 90%, a crosslinking effect in chemical bonds and among the chemical bonds of a collagen albumen product can be effectively removed after pepsase is used for performing hydrolysis on collagen fibers, so that maximally extracting the solvation collagen albumen can be realized; after the purification steps of sodium chloride salting out and dialysis, and the like are carried out, and the purity of the obtained collagen albumen product by extraction can reach more than 90%, the yield can reach 56.99-61.52% (dry weight), which is 97.1-102.5% of the total collagen albumen content of the black sea cucumber in East China Sea, so that the extraction of collagen albumen content with high productivity of the black sea cucumber in East China Sea can be realized.

The invention discloses a method for manufacturing brick by utilizing sludge from the East China Sea. The method includes that sea sludge and sea sand at the coastwise beach and sea port beach of the East China Sea are excavated and piled, natural dehydration weathering is carried out, sea sludge and sea sand which are subject to weathering are mixed into pug in proportion, and stirring, homogenizing, ageing, purifying, fine grinding, blanking, drying and roasting are carried out. The invention overcomes the defects that coefficient of drying sensitivity is high, plasticity index is high, brick body is easy to crack and shrink rate is high which are caused by the manufacturing in the prior art, natural resource is utilized and waste material is changed into things of value while reaching the national standards GB13544-2000 and GB13545-2003, thus not only saving a mass of tilled land resource but also solving the problems in wall material reformation and building energy conservation emission reduction project in brick making industry in coastal area.

The invention relates to the technical field of marine microorganisms. Since milk is generally conveyed and preserved at a low temperature, the capability of decomposing lactose at the low temperature is one of conditions which are necessary for industrially applying the beta-galactosidase. A marine halomonas strain P6006-1 provided by the invention is separated from sea water of an East China Sea region through culturing and has been preserved at the China Center for Type Culture Collection (CCTCC for short) in Wuhan city on January 7th, 2011, with a collection number of CCTCC No. M2011001. The invention also provides a method for producing the low-temperature beta-galactosidase by using the marine halomonas. The low-temperature beta-galactosidase produced by using the marine halomonas can be used for decomposing the lactose in cow milk; and the most proper reaction temperature is 30 DEG C.

The present invention relates to a dry production method of East China Sea Cucumaria japonica. The dry production method comprises: (1) pre-treating; (2) after an acupuncture treatment, placing into an improving liquid to immerse; (3) carrying out heating shaping by using a stepped heating manner; (4) carrying out primary drying by using an infrared drying method; (5) re-wetting; and (6) carrying out secondary drying by using a microwave vacuum drying method. According to the present invention, physical acupuncture, improving immersing with the improving liquid, stepped heating shaping, primary drying, re-wetting, secondary drying and other physical and biological methods are combined, such that the muscle fiber thinning atrophy is effectively inhibited, the prepared Cucumaria japonica dried product has the complete and full shape, the soaking time is significantly shortened (less than 10 h), no irritating odor or corruption odor is generated after soaking, and the soft and sticky taste is provided during eating and meets tastes of most people.

The invention discloses a device for simulating a whole cataclysm process of seabed soft soil under combined effects of upper-surface typhoon waves and pushing of a shallow buried high pressure gas, which can reproduce a weakening and pneumatic inrushing cataclysm whole process of the seabed soft soil under combined effects of the upper-surface typhoon waves and the pushing of the shallow buried high pressure gas. The device mainly comprises an outer model box, a soil model box, a perforated separator, a micro jack, a pneumatic cabin, a pneumatic servo motor, a shaft lever, a torque motor, a sliding guide rail and an impeller. A key physical simulation means is provided for revealing the seabed instability and cataclysm mechanism of three major waters (the Yellow Sea, the East China Sea and the South China Sea) with typhoon waves bursting and with high-pressure biogenic gas-containing seabeds distributed widely.

The invention relates to a manufacturing method for sea cucumber essence pure powder. The method comprises the following steps: first, grinding East China Sea black sea cucumbers which are subjected to decontaminating and cleaning treatment into pulp by using a stainless steel grinder; adding the pulp into a stainless steel enzymolysis tank through a 40-mesh screen; adding tap water with the weight being 1.8 to 2.0 times weight of the East China Sea black sea cucumbers; performing heat-insulating heating at 95 DEG C for half an hour; closing steam; starting an enzymolysis tank stirring machine, and enabling the enzymolysis tank stirring machine to rotate by 30 rotations per minute; turning on enzymolysis tank jacket cooling water to decrease the temperature of materials to 45 DEG C; adding Alcalase proteolytic enzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, Protamex compound protease with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, and Flavourzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers into the materials; starting the enzymolysis tank stirring machine, and performing heat-insulating hydrolysis for four hours at 45 to 47 DEG C; filtering and concentrating the materials after enzyme hydrolysis is ended, wherein the temperature of the materials is controlled to be 55 to 58 DEG C, and concentrating is not ended until the solid content is 25 to 30 percent; performing spray-drying by using a centrifugal spray-drying machine, thus obtaining a finished product, namely the sea cucumber essence pure powder, wherein the temperature at the inlet of the spray-drying machine is controlled to be 210 to 215 DEG C, and the temperature at the outlet of the spray-drying machine is controlled to be 95 to 96 DEG C.

The invention provides an arthrobacter strain ZJ-H and an application of the arthrobacter strain ZJ-H to the degradation of petroleum hydrocarbons in a high-saline-alkali environment. The strain ZJ-His selected from oil-based drilling cutting samples of the Bohai sea oilfield, which are provided by a China sea petroleum Bihai environmental protection technology service company. The strain ZJ-H isstrong in tolerance to the high-saline-alkali environment and can normally survive in the environment with the salinity of 1-5% and the pH value of 7-11; the strain ZJ-H has relatively high petroleumdegradation rate in the saline-alkali environment, which reaches 74.45%; a metabolic product of the strain ZJ-H has relatively high emulsifying activity, has a good emulsifying effect on petroleum ether, and is beneficial to further decomposition and utilization of the petroleum ether by the strain; and the strain ZJ-H has a good degradation capability for n-alkanes (including long-chain alkanesof C20-C30) in raw petroleum, the degradation rate exceeds 83%, and especially, the degradation rate of C25-C30 reaches about 95%.

The invention relates to the field of marine biotechnology, and provides a marine-microbe-sourced low-temperature beta-galactosidase, which is obtained by fermentation culturing and purification of a marine halomonas sp. strain P6009-1 obtained by the applicant previously. The marine halomonas sp. strain P6009-1 is cultured and separated from seawater in East China Sea areas, and has a collection number of CCTCC No: M2011001. Under a temperature of 0 DEG C, a capacity of the enzyme in catalyzing lactose hydrolysis is 20% of a maximal activity, such that good low-temperature performance is shown. The invention further provides a coding gene of beta-galactosidase, and an application of beta-galactosidase in decomposing milk lactose.

The application relates to a hybrid seedlings-cultivating method of the stichopus japonicus. The method comprises the steps of: firstly taking the stichopus japonicus in the China sea area and the black stichopus japonicus in the Japan northern sea area as the bred stichopus japonicus, and synchronously fertilizing the bred stichopus japonicus of the China stichopus japonicus and the bred stichopus japonicus of the Japan stichopus japonicus by controlling the cultivating water temperature and the bait feeding quantity of the bred stichopus japonicus in the process of artificial fertilizing and cultivating; artificially spawning and discharging sperm under the oxytocic condition of drying in the shade and warming, and respectively collecting the ovum from the Chinese stichopus japonicus and the sperm from the Japan black stichopus japonicus or the sperm from the Chinese stichopus japonicus and the ovum from the Japan black stichopus japonicus for the aritificial exomixis; and performing the incubation and the seedlings cultivation to the oosperm which is generated by hybridizing the two different geographical colonies which are reproductively isolated from each other according to the normal mode for cultivating the seedlings of the stichopus japonicus so as to cultivate the hybrid stichopus japonicus seedlings.

The invention discloses an offshore area wave power density parameterization calculation method for the China Sea. The method includes the following steps that firstly, a wave power density polynomial model is established; then, the significant wave height, an energy period and the wave power density which correspond to multiple offshore areas are acquired from MASNUM wave numerical models of the offshore areas in a whole year of a certain year; the significant wave height, the energy period and the wave power density serve as parameters of the polynomial model and the wave power density polynomial model is fit; finally, the polynomial model is used for accurately evaluating offshore area wave energy resources. By the adoption of the method, the wave power density can be calculated through the parameters of waves.

The invention discloses a feeding device and method for silvery pomfret in the East China Sea, and aims to overcome the defects that in the silvery pomfret breeding process, the utilization rate of baits is low, and the baits sink to the bottom of a pond for a long time to pollute the breeding environment. The feeding device comprises a feeding barrel, a plurality of bait outlet nozzles are arranged at the lower portion of the feeding barrel, one-way cover pieces are installed at the opening ends of the bait outlet nozzles, a piston assembly is installed in the feeding barrel, a bait storage barrel, a lifting base and a driving assembly are installed on the upper portion of the feeding barrel, the lifting base is connected with the piston assembly, a material squeezing plug is installed inthe bait storage barrel, and a piston rod is connected betwene the material squeezing plug and the lifting base; a material extruding cavity is formed between the position, below the piston assembly,in the feeding barrel and the bottom of the feeding barrel, and a feeding pipe is connected between the piston assembly and a discharging hole; the lifting base moves upwards, the material squeezingplug pushes the baits to enable the baits to be injected into the material squeezing chamber through the feeding pipe, and meanwhile the lifting base drives the piston assembly to move upwards to enable the material squeezing chamber to be enlarged; and the lifting base moves downwards to drive the piston assembly to move downwards to extrude the baits in the material squeezing chamber out throughthe bait outlet nozzles.

A harmful red tide organism cell surface specific antibody and a preparation method thereof relate to an antibody. The invention provides a harmful red tide organism cell surface specific antibody and a preparation method thereof. The antibody is an immunoglobulin which is produced by the plasma cells differentiated from B cell and can be combined and reacted with corresponding antigen in a specific way under the incentive of a red tide algae surface antigen substance. Firstly, East China Sea chain prorocentrum Alexandrium whole-cell surface antigen which is not contaminated by the protein in the cell is prepared and then mixed with complete Freund-type adjuvant, later, the mixture is injected into New Zealand white rabbits, and then antigen is injected and injection is enhanced; New Zealand white rabbit ear vein blood is collected 3 to 8 days before the serum antibodies are collected, and later the generation condition of antibodies is detected; the whole rabbit blood is collected 3 to 5 days after the last injection; and after standing and centrifugation, the serum is collected and then packaged and stored. Aiming at the red tide of algae type, corresponding red-tide algae species can be rapidly detected by using the antibody; and the detection limit can reach more than a dozen cells.

The invention discloses a production method of sea cucumber mucopolysaccharides. The production method of the sea cucumber mucopolysaccharides comprises the following steps: 1, adding filter residuesproduced during production processes of East-China-Sea Wushen (dark sea cucumbers) collagen into a stainless steel centrifuge so as to carry out dehydration; 2, adding the dehydrated filter residues into a reactor, adding sodium bicarbonate according to a proportion, and turning on a mixer at normal temperature; 3, performing press filtration on dissolved liquid by using a plate-frame filter press, and carrying out fine filtration by using a membrane filter; 4, collecting the filtrate, turning on the mixer, turning off the mixing, carrying out standing for 4 hours, and collecting filter cakesof sea cucumber mucopolysaccharides; and 5, dispersedly tiling the collected filter cakes of the sea cucumber mucopolysaccharides in a stainless steel basin, carrying out drying in a drying shed witha heat-pump dryer so as to carry out low-temperature drying, and then, carrying out checking so as to obtain sea cucumber mucopolysaccharide products. The production method of the sea cucumber mucopolysaccharides has the following beneficial effects: environmental pollution caused by high-protein-content sewage discharged during production processes of enzymatic hydrolysis or alkali dissolution ofsea cucumber raw-materials in traditional methods is reduced so that production costs of the sea cucumber mucopolysaccharides can be greatly reduced; moreover, the East-China-Sea Wushen resources areabundant so that the high-end consumer goods, namely the sea cucumber mucopolysaccharides, becomes mass consumer goods.