62 results about "China sea" patented technology

The invention discloses a comprehensive hydrate simulation system and an experimental method thereof. Generally, the simulation system includes a negative pressure sample chamber for placing a sample,and a confining pressure system connected to the negative pressure sample chamber, a parameter measurement system, a constant temperature system, a vacuum system, a gas injection system, a liquid injection system, a heat injection system, a drilling fluid circulation system, a return pressure system, and a data acquisition processing unit. The simulation system of the invention can simulate exploration of natural gas hydrates in all aspects, can be applied in indoor research and synthesis of natural gas hydrates in China sea areas and decomposition mechanisms of natural gas hydrates, and canprovide support for physical simulation of reservoir physical properties, temperature, pressure, laws of yield changes and other important sensitive parameters affecting trial exploration of sea hydrates during the trial exploration processes in different development modes and different development well group conditions.

The invention relates to a method for manufacturing collagen from black sea cucumbers from East China Sea, which comprises the following steps: (1) adding Alcalase hydrolysis protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea, protamex composite protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea and flavourzyme in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea into the black sea cucumbers from East China Sea which are subjected to foul-removing and cleaning treatment, heating the materials to 45 to 47 DEG C, and hydrolyzing at a constant temperature of 45 to 47 DEG C with stirring for 4 hours; and (2) after enzyme hydrolysis is finished, performing fine filtration by a membrane filter, concentrating in a vacuum concentrator, keeping the temperature of the materials in the vacuum concentrator between 55 and 58 DEG C by controlling the heating steam valve of the vacuum concentrator, and concentrating to obtain pasty or powder collagen product from black sea cucumbers from East China Sea. The method has the advantages that: a technical route of combination of three enzymes and hydrolysis degree intermediate detection is adopted, so the relative molecular weight of the product of the enzyme hydrolysis of the black sea cucumbers from East China Sea can be controlled to meet the requirement on the relative molecular weight of sea cucumber collagen products; and the product of the hydrolysis product is free from bitter taste and fishy smell.

The invention discloses a decortication method for sea cucumber in East China Sea, including the steps of the preliminary rehydration and softening of the sea cucumber, cleaning and decontamination, pressurization and cooking, swelling at lower temperature, soaking in the decortication solution, swelling again, soaking in the decortication solution again and the like. In the swelling process, the crass cuticle of the sea cucumber in East China Sea can be completely removed, the sea cucumber body is clean and glistened, having no influence on the edible part of the inner layer wall and keeping the nutrient composition of the sea cucumber in East China Sea.The obtained sea cucumber product after subsequent processing has no fishy smell, good taste, rich nutrition, delicious taste, being neither too hard nor too softand stored for a long time after further packing treatment.

The invention relates to a method for performing inversion on the optical property of aerosol of a coastal zone, and provides a new algorithm for performing inversion on the optical property of the aerosol of a coastal area in China by using a satellite image aiming at the characteristics of a large number of aerosol particles with high reflectivity and strong absorbability on the ocean surface of the coastal area in China. In the algorithm, the research is conducted by taking an image of a moderate resolution imaging spectroradiometer (MODIS) as an example; the inversion of the optical property of atmospheric aerosol over a muddy water area is finished by using the images of long wave bands of 1,240 nm, 1,640nm and 2,133 nm of the MODIS and a lookup table; and the correctness and applicability of the algorithm are verified by using an foundation observation result of a cleaning day. Theoretical basis is provided for adding a channel with long wave bands and transmissivity into a Chinese sea color remote sensor, the condition of the aerosol over the coastal zone can be reflected in real time, and decision basis is provided for related functional departments.

The invention discloses a liquid fermentation method for producing laccase by using marine endogenous pestalotia, which comprises the following steps of: (1) screening a strain: screening soil in the depth of 10-20m on the offshore of the East China Sea to obtain a novel marine fungi; (2) preserving the strain: inoculating the strain in a modified potato dextrose agar (PDA) medium for culture, and preserving on a slope at the temperature of 4DEG C; (3) activating and propagating the strain: inoculating a spore of the strain in a PDA medium added with special components, and inoculating the activated spore in a seed culture medium for propagation; (4) preparing a marine endogenous pestalotia liquid fermentation medium; and (5) fermenting: performing liquid fermentation and enzyme production by using the newly prepared medium, and determining the optimized enzyme production condition. In the method for producing the laccase, the novel marine endogenous pestalotia is taken as a production strain; and the method has the characteristic that the media are coarse, wide in sources, low in prices and the like.

The invention relates to an aquaculture method of deep sea scallops, in particular to a breeding and propagation method for Atlantic deep sea scallops. The method comprises the steps that introduction, artificial breeding and aquaculture of the deep sea scallops are carried out, parent scallops are mature after 45-50 days maturity promoting and lay eggs, and after most larvae grow into D-shaped larvae, a bolting cloth net cage is used for selecting optimal larvae to a new pond for cultivation; the growth rate of the cultured larvae is far higher than that of the larvae cultured in a nursery ground in Quebec of Canada at the same time, eyespots are generated on most larvae 16-17 days after the larvae are fertilized, adhering substances are arranged at the 18th day, after the adhering substances are arranged for 10 days, the adhering substances are taken out and hung in natural sea ears for larva protection; the deep sea scallops meet commercial specification when the cultivated average specification reaches 8 centimeters, and the deep sea scallops achieve sexual maturity. According to the method, the Atlantic deep sea scallops are introduced into China sea areas for the first time, the scallop culture species are expanded, and construction of modern ocean ranches is promoted.

The invention relates to a hybrid breeding method for stichopus japonicus, comprising the following steps: selecting stichopus japonicus in China sea areas and in Korea sea areas as parental stichopus japonicus, respectively controlling breeding water temperature, times and time of feeding of the parental stichopus japonicus in an artificial culture, and adding yolk and pachyrhizus powder of different amounts for stimulating gonadogenesis in breeding bait containing alga powder, compound feed and holothurian vitamin to enable synchronous maturity of gonads of the parental stichopus japonicus; stimulating the parental stichopus japonicus by a shade-drying running water stimulating method, a temperature differential method and an artificial induced spawning method combined with a method of using spermia to stimulate ovum, to induce the oviposition and the spermiation of the parental stichopus japonicus; and respectively collecting the ovum and the spermia of the parental stichopus japonicus; carrying out reciprocal hybridizations to produce oosperm; and breeding according to a conventional method. The stichopus japonicus sprout cultured by the method has high survival rate, fast growth rate, good stress resistance, and strong ability of resisting mechanical damage. The process is easy and convenient, and can be operated easily, effectively shortens the period of breeding the stichopus japonicus and saves the breeding cost.

A process for culturing the Bacillus subtilis coming from sea to obtain the insecticiding product for more agricultural pests includes bioactive screening to the marine microbes in Chinese sea to obtain a high-activity Bacillus subtilis strain, and culturing it in fermenting culture medium to obtain said active insecticiding product. Its advantages are high killing activity to agricultural pests, broad spectrum, and no environmental pollution.

The present invention relates to seawater aquiculture technology, and is especially pond cultivation technology of East China sea lefteye mandarin. The pond cultivation technology includes adopting wild East China sea lefteye mandarin as parent fishes; promoting mature and induced spawning of parent fishes through controlling lighting, water temperature and reinforced nutrients; and sea water aquiculture, during which underground sea water is utilized in the high temperature period. The present invention has high survival rate of fry and young East China sea lefteye mandarin as one new aquiculture variety.

The invention relates to a method for calculating high-precision Chinese offshore sea wave characteristic distribution. According to the method, a meteorological model WRF, a large-area sea wave modelwavewatch 3 and a sea wave model swan used in the offshore area of China are combined, and a multi-year historical sea wave characteristic data product with the spatial resolution of 0.04 degree * 0.04 degree can be calculated. As the China offshore area is divided into the East China sea area and the South China sea area by considering the monsoon influence area and the wave propagation characteristics during model establishment. The western pacific ocean is prevented from being brought into a mode range, and computing resources are prevented from being consumed. Due to the fact that the twosea areas have the overlapping area, the overlapping area has two calculation results, a weighted average method is used, the calculation results of the two overlapping areas are integrated, and thewhole set of data has spatial continuity.

The invention discloses an ecological risk assessment method of a polycyclic aromatic hydrocarbon compound and a mixture thereof in a marine water environment. The assessment method comprises the following steps of determining the type and concentration of the polycyclic aromatic hydrocarbon in the marine water environment; screening the representative biological species having the flora characteristics of the creatures of the China sea area; acquiring the chronic toxicity data of polycyclic aromatic hydrocarbon in the water body of a target sea area; using a statistical extrapolation method to calculate the predicted no effect concentration of polycyclic aromatic hydrocarbon; using a joint probability curve method to calculate the level of the ecological risk of single polycyclic aromatic hydrocarbon in the water body of the target sea area; using a toxicity equivalent factor method to calculate the joint ecological risk of the polycyclic aromatic hydrocarbon mixture in the water body of the target sea area; identifying the risk of the monomer and the mixture of the polycyclic aromatic hydrocarbon according to the above results. The ecological risk assessment method of the polycyclic aromatic hydrocarbon compound and the mixture thereof in the marine water environment provided by the invention realizes the ecological risk assessment of the polycyclic aromatic hydrocarbon monomer and the mixture thereof in the China marine water environment by using the toxicity equivalent and the probability risk assessment method based on the species sensitivity distribution theory, and provides a decision basis for the marine ecological risk management of the China organic pollutants.

The invention relates to the technical field of medicines and provides a new method for separating a polysaccharide compound from mytilus crassitesta lischlk produced in the East China sea and purifying the polysaccharide compound. The invention also provides application of the polysaccharide compound in preparation of an anti-tumour drug.

The invention provides a method for extracting and purifying pepsin-soluble collagens of black sea cucumbers in East China Sea. The method comprises the following steps: soaking a clean, massive black sea cucumber sample in East China Sea with an EDTA (Ethylene Diamine Tetraacetic Acid) solution; performing homogenized homogeneity; performing extraction in sequence by using a Tris-HCl solution, a NaOH solution and a pepsase acetic acid solution; and performing salting out and dialysis to obtain the pepsin-soluble collagens. According to the invention, the EDTA chelating agent is used to remove chemical element with high pollution concentration from sea cucumber tissues, and the removal efficiency can reach more than 90%, a crosslinking effect in chemical bonds and among the chemical bonds of a collagen albumen product can be effectively removed after pepsase is used for performing hydrolysis on collagen fibers, so that maximally extracting the solvation collagen albumen can be realized; after the purification steps of sodium chloride salting out and dialysis, and the like are carried out, and the purity of the obtained collagen albumen product by extraction can reach more than 90%, the yield can reach 56.99-61.52% (dry weight), which is 97.1-102.5% of the total collagen albumen content of the black sea cucumber in East China Sea, so that the extraction of collagen albumen content with high productivity of the black sea cucumber in East China Sea can be realized.

The invention relates to a laurane type sesquiterpene compound or a derivative thereof extracted and separated from a sea plant Laurencia okamurai in the East China Sea, and a preparation method and application thereof. The structural formula of the laurane type sesquiterpene compound or the derivative thereof is shown as above: when R1, R2 and R3 are hydrogen at the same time, the sesquiterpene compound or the derivative thereof is laurinferol, and when R4 is hydrogen, the sesquiterpene compound or the derivative thereof is aplysin. In vitro tests show that the sesquiterpene compound or the derivative thereof has obvious pancreatic lipase inhibition activity and is expected to be applied to preparing medicaments for preventing or treating decompensation diseases including adiposis, diabetes, hyperlipoidemia, fatty liver and the like. The sesquiterpene compound or the derivative thereof can also provide a lead compound for developing the new medicaments for preventing and treating the decompensation diseases, and is significant for developing and utilizing marine biological resources of China.

The invention relates to the technical field of medicament and discloses a new application of mussel polysaccharide separated from extract of marine life mussels in preparing anti-ageing medicament or foods. The mussel polysaccharide is extracted from fresh mussel meat of mytilus crassitestas in the East China Sea, can respectively improve activity of SOD and GSH-px in blood serum, livers and brain tissues and lower content of MDA. Therefore, the mussel polysaccharide has anti-ageing activity and can be used to prepare anti-ageing medicament or foods. The invention provides a new application to mussel polysaccharide which is helpful to explore and use marine life.

The invention relates to the technical field of marine microorganisms. Since milk is generally conveyed and preserved at a low temperature, the capability of decomposing lactose at the low temperature is one of conditions which are necessary for industrially applying the beta-galactosidase. A marine halomonas strain P6006-1 provided by the invention is separated from sea water of an East China Sea region through culturing and has been preserved at the China Center for Type Culture Collection (CCTCC for short) in Wuhan city on January 7th, 2011, with a collection number of CCTCC No. M2011001. The invention also provides a method for producing the low-temperature beta-galactosidase by using the marine halomonas. The low-temperature beta-galactosidase produced by using the marine halomonas can be used for decomposing the lactose in cow milk; and the most proper reaction temperature is 30 DEG C.

The present invention relates to a dry production method of East China Sea Cucumaria japonica. The dry production method comprises: (1) pre-treating; (2) after an acupuncture treatment, placing into an improving liquid to immerse; (3) carrying out heating shaping by using a stepped heating manner; (4) carrying out primary drying by using an infrared drying method; (5) re-wetting; and (6) carrying out secondary drying by using a microwave vacuum drying method. According to the present invention, physical acupuncture, improving immersing with the improving liquid, stepped heating shaping, primary drying, re-wetting, secondary drying and other physical and biological methods are combined, such that the muscle fiber thinning atrophy is effectively inhibited, the prepared Cucumaria japonica dried product has the complete and full shape, the soaking time is significantly shortened (less than 10 h), no irritating odor or corruption odor is generated after soaking, and the soft and sticky taste is provided during eating and meets tastes of most people.

The invention discloses a solid fermentation method for producing salt-tolerant cellulase by using an oceanic Aspergillus niger, comprising the following steps of: (1) screening an oceanic Aspergillus niger from soil in the depth of 10-20m approaching to the East China Sea; (2) vaccinating the oceanic Aspergillus niger on a PDA culture medium for culturing to obtain an inclined-plane spore, and preserving the inclined-plane spore at 4 DEG C; (3) vaccinating the inclined-plane spore on a PDA-1 culture medium for culturing to obtain an activated spore; (4) vaccinating the activated spore on an eggplant bottle inclined plane for culturing for 74h at 42 DEG C to obtain an eggplant bottle inclined-plane spore; and (5) propagating the obtained eggplant bottle inclined-plane spore, diluting the eggplant bottle inclined-plane spore to 108 spores / ml by using sterilized artificial seawater, vaccinating to a solid fermentation enzyme-producing culture medium, and fermenting for 8-12 days to obtain the cellulase. The solid fermentation method has the characteristics of coarse culture medium, wide source, low price and the like, the produced cellulase can degrade cellulose under the condition of a higher salt content, and the enzyme is abnormally stable under high-salt environment, therefore, the produced cellulase lays the foundation for the subsequent application of hydrolysate.

The invention relates to the technical field of polypeptide extraction accessory devices, and in particular relates to equipment for extracting polypeptides in sea cucumbers in the East China Sea. Theequipment comprises a hollow stirring kettle, a first threaded rod and a second threaded rod; a first bearing is arranged in the central area of the top of the stirring kettle; the inner ring of thefirst bearing is connected with a first stirring shaft; multiple first stirring blades are arranged on the outer side wall of the first stirring shaft; the interior of the first stirring shaft is hollow; a mechanical seal is arranged at the bottom in the first stirring shaft; a second bearing is arranged on the top in the first stirring shaft; a second stirring shaft is arranged on the inner ringof the second bearing; the second stirring shaft is rotatably connected with the mechanical seal; multiple second stirring blades (34) are arranged in the lower half area of the second stirring shaft;and a first bevel gear is arranged on the first stirring shaft. The equipment in the invention is simple in structure; the mixing effect and the mixing speed of a freeze-drying protective agent and semi-finished polypeptide concentrated solution are improved; the polypeptide concentrated solution and the freeze-drying protective agent on the inner wall are convenient to clean; and the product quality is improved.

The invention discloses a device for simulating a whole cataclysm process of seabed soft soil under combined effects of upper-surface typhoon waves and pushing of a shallow buried high pressure gas, which can reproduce a weakening and pneumatic inrushing cataclysm whole process of the seabed soft soil under combined effects of the upper-surface typhoon waves and the pushing of the shallow buried high pressure gas. The device mainly comprises an outer model box, a soil model box, a perforated separator, a micro jack, a pneumatic cabin, a pneumatic servo motor, a shaft lever, a torque motor, a sliding guide rail and an impeller. A key physical simulation means is provided for revealing the seabed instability and cataclysm mechanism of three major waters (the Yellow Sea, the East China Sea and the South China Sea) with typhoon waves bursting and with high-pressure biogenic gas-containing seabeds distributed widely.

The invention relates to a manufacturing method for sea cucumber essence pure powder. The method comprises the following steps: first, grinding East China Sea black sea cucumbers which are subjected to decontaminating and cleaning treatment into pulp by using a stainless steel grinder; adding the pulp into a stainless steel enzymolysis tank through a 40-mesh screen; adding tap water with the weight being 1.8 to 2.0 times weight of the East China Sea black sea cucumbers; performing heat-insulating heating at 95 DEG C for half an hour; closing steam; starting an enzymolysis tank stirring machine, and enabling the enzymolysis tank stirring machine to rotate by 30 rotations per minute; turning on enzymolysis tank jacket cooling water to decrease the temperature of materials to 45 DEG C; adding Alcalase proteolytic enzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, Protamex compound protease with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, and Flavourzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers into the materials; starting the enzymolysis tank stirring machine, and performing heat-insulating hydrolysis for four hours at 45 to 47 DEG C; filtering and concentrating the materials after enzyme hydrolysis is ended, wherein the temperature of the materials is controlled to be 55 to 58 DEG C, and concentrating is not ended until the solid content is 25 to 30 percent; performing spray-drying by using a centrifugal spray-drying machine, thus obtaining a finished product, namely the sea cucumber essence pure powder, wherein the temperature at the inlet of the spray-drying machine is controlled to be 210 to 215 DEG C, and the temperature at the outlet of the spray-drying machine is controlled to be 95 to 96 DEG C.

The invention provides an arthrobacter strain ZJ-H and an application of the arthrobacter strain ZJ-H to the degradation of petroleum hydrocarbons in a high-saline-alkali environment. The strain ZJ-His selected from oil-based drilling cutting samples of the Bohai sea oilfield, which are provided by a China sea petroleum Bihai environmental protection technology service company. The strain ZJ-H isstrong in tolerance to the high-saline-alkali environment and can normally survive in the environment with the salinity of 1-5% and the pH value of 7-11; the strain ZJ-H has relatively high petroleumdegradation rate in the saline-alkali environment, which reaches 74.45%; a metabolic product of the strain ZJ-H has relatively high emulsifying activity, has a good emulsifying effect on petroleum ether, and is beneficial to further decomposition and utilization of the petroleum ether by the strain; and the strain ZJ-H has a good degradation capability for n-alkanes (including long-chain alkanesof C20-C30) in raw petroleum, the degradation rate exceeds 83%, and especially, the degradation rate of C25-C30 reaches about 95%.

The invention relates to the field of marine biotechnology, and provides a marine-microbe-sourced low-temperature beta-galactosidase, which is obtained by fermentation culturing and purification of a marine halomonas sp. strain P6009-1 obtained by the applicant previously. The marine halomonas sp. strain P6009-1 is cultured and separated from seawater in East China Sea areas, and has a collection number of CCTCC No: M2011001. Under a temperature of 0 DEG C, a capacity of the enzyme in catalyzing lactose hydrolysis is 20% of a maximal activity, such that good low-temperature performance is shown. The invention further provides a coding gene of beta-galactosidase, and an application of beta-galactosidase in decomposing milk lactose.

The invention relates to the technical field of medicines and provides a new partially acidic hydrolyzed compound of East China Sea mytilus coruscus polysaccharides. The invention also provides a preparation method and an application of the partially acidic hydrolyzed compound of the mytilus coruscus polysaccharides.

The application relates to a hybrid seedlings-cultivating method of the stichopus japonicus. The method comprises the steps of: firstly taking the stichopus japonicus in the China sea area and the black stichopus japonicus in the Japan northern sea area as the bred stichopus japonicus, and synchronously fertilizing the bred stichopus japonicus of the China stichopus japonicus and the bred stichopus japonicus of the Japan stichopus japonicus by controlling the cultivating water temperature and the bait feeding quantity of the bred stichopus japonicus in the process of artificial fertilizing and cultivating; artificially spawning and discharging sperm under the oxytocic condition of drying in the shade and warming, and respectively collecting the ovum from the Chinese stichopus japonicus and the sperm from the Japan black stichopus japonicus or the sperm from the Chinese stichopus japonicus and the ovum from the Japan black stichopus japonicus for the aritificial exomixis; and performing the incubation and the seedlings cultivation to the oosperm which is generated by hybridizing the two different geographical colonies which are reproductively isolated from each other according to the normal mode for cultivating the seedlings of the stichopus japonicus so as to cultivate the hybrid stichopus japonicus seedlings.

The invention discloses an offshore area wave power density parameterization calculation method for the China Sea. The method includes the following steps that firstly, a wave power density polynomial model is established; then, the significant wave height, an energy period and the wave power density which correspond to multiple offshore areas are acquired from MASNUM wave numerical models of the offshore areas in a whole year of a certain year; the significant wave height, the energy period and the wave power density serve as parameters of the polynomial model and the wave power density polynomial model is fit; finally, the polynomial model is used for accurately evaluating offshore area wave energy resources. By the adoption of the method, the wave power density can be calculated through the parameters of waves.