57 results about "Petroleum ether" patented technology

The application provides a regeneration method of halogenated nitrobenzene continuous hydrogenation catalyst, and relates to the technical field of fixed bed halogenated nitrobenzene hydrogenation. The regeneration method mainly comprises the following steps: using alcohol, alkali, petroleum ether and ethyl acetate to preliminarily clean the bed layer, removing azo compounds and other pollutants on the surface of the catalyst by using the special properties of ionic liquids, and then completing the regeneration of the catalyst through the steps of pickling, precious metal solution treatment and drying. The application overcomes the defects of the prior art, has the advantages of simple operation, mild conditions and low cost, realizes the in-situ removal of surface impurities such as reaction intermediates and azo compounds on the catalyst surface without damaging the structure of the catalyst and disassembling the catalyst, further supplements the lost metal active components, and can effectively restore the catalyst activity.

The application discloses a hot air pretreatment method for extracting oat oil and belongs to the technical field of oil extraction. The application solves the technical problems of low extraction efficiency, easy oxidation of oil and large solvent consumption existing in the prior art. The method comprises the following steps: a. oat bran is dried by hot air at 130-150 DEG C for 15-20 min; b. the oat bran is placed in a negative pressure environment, and ethanol vapor flash treatment is performed for 5-15 min; c. the oat bran is mixed with a mixed solvent of petroleum ether and ethanol, immobilized lipase and natural antioxidant are added, and extraction is performed for 8-12 h, and then filtration separation is performed; d. the first residue is mixed with the mixed solvent, extraction is performed for 4-6 h, and then filtration separation is performed; e. the first supernatant and the second supernatant are combined, the solvent is recovered by reduced pressure distillation, and oat crude oil is obtained; and f. the immobilized lipase is recovered. The method is mainly used for efficiently extracting high-quality oat oil from oat bran.

The application discloses a pterosin sesquiterpenoid compound and a preparation method and application thereof, belongs to the technical field of biological medicines, aims at the problems of a research blank of pterosin sesquiterpenoid compounds in Anemone raddeana and a lack of structure-confirmed anti-inflammatory active monomers, and provides a pterosin sesquiterpenoid compound with a structural formula as shown in the following.The compound preparation needs to first crush dried whole grass of Anemone raddeana, extract by heating and refluxing with an organic solution, concentrate under reduced pressure to obtain total extract; then, the extract is extracted by petroleum ether, ethyl acetate and n-butanol step by step, the ethyl acetate part extract is enriched, and the high-purity product is prepared through multi-step purification of a silica gel column, a C-18 reverse phase column, a gel column and semi-preparative liquid chromatography.In addition, the compound can dose-dependently inhibit the NO release of LPS-induced macrophages, and can be used for preparing anti-inflammatory drugs for treating or preventing inflammatory diseases related to excessive production of nitric oxide, fills the research blank, and provides a high-quality candidate component for anti-inflammatory drug research and development.

The present application relates to a surfactant composition containing an alkyl hydroxy ether acetate (A) represented by the following general formula (1) and a compound (I) detected between retention time 10 minutes and 17 minutes in a gas chromatography using petroleum ether in conformity with JIS K8593, the ratio of the peak area of the compound (I) to the peak area of the petroleum ether determined by the gas chromatography being 0.02% to 50%.R 1 -CH(OH)-CH2-OCH2COOM 1 (1) [In the general formula (1), R 1 represents an alkyl group having 8 to 12 carbon atoms, M 1 represents a hydrogen atom, a sodium atom, a potassium atom or triethanolammonium.]

This invention relates to the field of rose separation and purification technology, specifically a novel benzofuran compound from rose petals, its extraction method, and its application. The method involves extracting the total rose extract obtained by heating and reflux of dried rose petals, then extracting the petroleum ether fraction. After elution and separation, the third fraction is further eluted with silica gel, the second fraction is eluted again, and the third fraction is purified by elution. The novel benzofuran compound from rose petals is obtained at 21.0 minutes. This invention discloses for the first time the extraction and isolation of a benzofuran compound from rose petals, and its antibacterial pharmacodynamic effects were tested. The experiments clearly showed that the compound has a strong inhibitory effect on Staphylococcus aureus and Escherichia coli, thus enabling its application in the preparation of antibacterial drugs and antibacterial health products.

This invention discloses a biphasic combined Lewis acid hydrolysis method for extracting diosgenin from fenugreek, belonging to the field of natural product extraction technology. The extraction method disclosed in this invention includes: defatting fenugreek raw material, mixing it with a biphasic reaction system composed of Lewis acid, concentrated hydrochloric acid, methanol, water, and petroleum ether, and carrying out a hydrolysis reaction under heating conditions. After the reaction, the organic phase is separated, concentrated, and dried to obtain diosgenin. This invention innovatively introduces a Lewis acid catalyst into the biphasic acid hydrolysis system of fenugreek, achieving significant results such as a 25% reduction in reaction time, a 50% reduction in concentrated hydrochloric acid usage, and a 25% reduction in water usage while ensuring the extraction rate of diosgenin. This method has advantages such as mild conditions, low acid consumption, short processing time, and environmental friendliness, and is suitable for the large-scale green production of diosgenin.

This invention discloses a valsartan disodium intermediate, belonging to the field of pharmaceutical intermediate technology. First, valsartan is reacted with an alkaline solution in a separate system to form a salt. Unreacted raw materials and poorly soluble impurities are removed by filtration and washing, resulting in a salt solution with a high proportion of effective components and a low content of crystal-inhibiting impurities. Subsequently, a solvent-controlled crystallization process using ethyl acetate and petroleum ether is employed, and ordered nucleation and crystal growth are achieved through staged cooling to obtain valsartan disodium crystals and a mother liquor. Secondary crystallization of the mother liquor is then performed using seed induction to fully extract and recover residual effective components, thereby improving raw material utilization and overall yield. This invention effectively reduces solvent loss, minimizes impurity interference, and enhances the stability of the crystallization process. It has the advantages of strong process controllability, high yield, and suitability for large-scale application.

The present application provides a kind of production solvent oil hydrogenation process, comprising the following steps: (1) reforming raffinate oil raw material is cut to obtain light component and heavy component;(2) the paraffin-containing raw material and heavy component enter the hydrofining reaction zone, and after the reaction is completed, hydrofining product oil is obtained;(3) hydrofining product oil is treated by stripping to obtain gas phase stream and liquid phase stream;(4) the liquid phase stream enters the de-aromatic reaction zone to carry out hydrodearomatization reaction to obtain a series of solvent oil products.The present application realizes the co-production of different types of high-quality solvent oils such as n-alkane / isomerized alkane solvent oil, pentane blowing agent, food additive n-hexane / industrial n-hexane, vegetable oil extraction solvent and food-grade petroleum ether based on the selection of raw materials and processing path, and the entire production process can be operated stably for a long period.

A system for the manufacture of pharmaceutical formulations for the treatment of diabetes and diabetic neuropathy, comprising: a) an extraction unit for producing an ethanolic extract from shade-dried leaves of Moringa oleifera Lam. and Bacopa monnieri Linn., and defatting the dried, crushed leaves with petroleum ether to remove lipids and chlorophyll; b) a fractionation unit configured to fractionate the ethanolic extract with ethyl acetate to obtain the ethyl acetate fraction; c) a phytosome preparation unit for the production of phytosomes using antisolvens precipitation technology, wherein the phytosome preparation unit comprises the following: • an ultrasonic device for sonicating a mixture of ethyl acetate fraction and phosphatidylcholine, • a heating device configured to heat the sonicated mixture below 40 °C, and • a condensation device configured to condense solvents into a complex film; and d) a formulation unit configured to incorporate the phytosomes into pharmaceutical dosage forms.

The application discloses a sesquiterpene dimer and a preparation method and application thereof, and belongs to the technical field of natural compound extraction. According to the preparation method, dried sesquiterpene dimers are extracted by refluxing with 95% ethanol, and then petroleum ether and ethyl acetate are used for extraction to obtain an extract, which can be obtained by column chromatography and semi-preparative high performance liquid chromatography, so that the method is simple, time and labor are saved, and the yield is higher. The obtained sesquiterpene dimers have high anti-neuritis activity, and have important application prospects for developing new drugs for treating senile dementia.

This invention discloses a thermally induced gel, its preparation method, and its application, belonging to the field of food colloids. It solves the problem that walnut meal protein obtained by existing methods cannot be used in food systems. Preparation method: Walnut meal is ground into powder, petroleum ether is added and stirred, and residual petroleum ether is removed by filtration to obtain defatted walnut meal powder; choline chloride and glycerol are mixed to form a transparent and homogeneous solution, obtaining DES solvent; defatted walnut meal powder is mixed with DES solvent, heated in a water bath and stirred, ethanol is added to the supernatant, the precipitate is refrigerated and dried to obtain DES-extracted walnut protein powder; the walnut protein powder is dispersed in water with a pH of 2.0 or 11.0; the resulting hydrated solution is heated in a water bath; and cooled to room temperature in an ice-water bath. This invention uses a DES solvent system to extract walnut meal protein under mild conditions, resulting in high protein purity, effective protection of the protein's native conformation, and gels exhibiting typical solid-state rheological characteristics with controllable mechanical strength.

The present application relates to the technical field of separation and purification of Schizonepeta tenuifolia Briq, and relates to a biphenyl compound in Schizonepeta tenuifolia Briq, a preparation method thereof and application of the biphenyl compound as an anti-inflammatory drug. The biphenyl compound in Schizonepeta tenuifolia Briq is obtained by the following steps: soaking Schizonepeta tenuifolia Briq, heating and reflux extraction, recovering and concentrating under reduced pressure, obtaining total extract of Schizonepeta tenuifolia Briq, extracting and concentrating with petroleum ether, dichloromethane and ethyl acetate, obtaining a dichloromethane part extract, gradient elution separation of the dichloromethane part extract, gradient elution separation of the fourth fraction of the obtained eight fractions, gradient elution separation of the third fraction of the obtained six fractions, gradient elution separation of the fifth fraction of the obtained eight fractions, purification and separation of the eluate, and collection of the eluate, and the biphenyl compound in Schizonepeta tenuifolia Briq is obtained at 16.4 minutes. The present application discloses the biphenyl compound in Schizonepeta tenuifolia Briq for the first time, and in vitro anti-inflammatory pharmacodynamic experiments are carried out, which prove that the biphenyl compound has a certain inhibitory effect on RAW264.7 cells and can be applied to preparation of anti-inflammatory drugs.

The present application relates to the technical field of tobacco processing, and in particular to a tobacco processing method for separating a top note, a body note, and a base note, and an aerosol generation substrate. The method comprises the following steps: S1: adding an extraction solvent to tobacco raw materials, and performing extraction at -18°C to -5°C to obtain a crude tobacco extract; S2: performing molecular distillation on the crude tobacco extract at 90-120°C to obtain a top note and a primary molecular distillation residue; S3: performing molecular distillation on the primary molecular distillation residue at 130-170°C to obtain a body note and a base note; and S4: respectively performing adsorption decolorization treatment on the top note, the body note, and the base note, wherein the extraction solvent in S1 comprises ethanol, petroleum ether, and ethyl acetate in a volume ratio of (1-7):(1-7):(1-2). The method can achieve efficient separation of the top note, body note and base note of tobacco, and the obtained respective notes have high aroma intensity and are naturally coordinated, facilitating supplementation of specific aromas according to blending requirements.

The application discloses application of a petroleum ether extract of a root of a roadside green plant in preparation of a drug for treating colorectal cancer by regulating a PI3K / AKT / MDM2 / p53 pathway. In the application, the petroleum ether extract of the root of the roadside green plant for treating colorectal cancer is obtained by petroleum ether reflux extraction of the root of the roadside green plant. Pharmacological experiments show that the petroleum ether extract of the root of the roadside green plant treats colorectal cancer by regulating the PI3K / AKT / MDM2 / p53 signal pathway through down-regulating p-PI3K / PI3K and p-AKT / AKT ratio, up-regulating p-MDM2 and down-regulating p53 level; the extract has an anti-malignant proliferation effect, induces G1 phase arrest by targeting CDK2, up-regulating p21, down-regulating Cyclin E1-CDK2 and Cyclin D1-CDK4; the extract induces HCT-116 cell apoptosis through a mitochondrial pathway by up-regulating Bax / Bcl-2, reducing ΔΨm, releasing Cyt C, activating Caspase-9 / 3 and cleaving PARP; the extract inhibits migration and invasion of HCT-116 cells by targeting MMP9, down-regulating MMP-2, MMP-9 and N-cadherin expression and up-regulating E-cadherin expression. In addition, the extract inhibits growth of a transplanted tumor in a nude mouse transplanted tumor model by inducing apoptosis of transplanted tumor cells.

The application provides a synthesis process of an ocinaplon intermediate. S1, petroleum ether, methanol and ethyl formate are mixed to obtain a mixed system, ortho-phenyltoluene and sodium methoxide are added, and reaction is carried out to obtain intermediate 1; S2, intermediate 1 is added into methanol, a palladium-carbon catalyst is added, hydrogen is introduced, and reaction is carried out to obtain intermediate 2; S3, phosphoric acid and a supported catalyst are added into intermediate 2, and reaction is carried out to obtain iminodibenzyl. The problem of low iminodibenzyl yield in the prior art is solved.

The present application relates to the technical field of separation and purification of Schizonepeta tenuifolia, and is a kind of Schizonepeta tenuifolia phenylpropanoid compound, its preparation method and application, Schizonepeta tenuifolia is extracted by heating reflux with ethanol solution and recovered under reduced pressure, and concentrated, the total extract of Schizonepeta tenuifolia is extracted and concentrated with petroleum ether, dichloromethane and ethyl acetate in turn, the extract of ethyl acetate part is separated by gradient elution, the third fraction is separated by gradient elution, the first fraction is separated by gradient elution, the sixth fraction is separated by gradient elution, and phenylpropanoid compound one and phenylpropanoid compound two are obtained at 12.1 minutes and 13.4 minutes, respectively. The present application discloses phenylpropanoid compound one and phenylpropanoid compound two separated from Schizonepeta tenuifolia for the first time, and in vitro anti-inflammatory pharmacodynamic experiments are carried out on the two compounds, which prove that they have a certain inhibitory effect on RAW264.7 cells, and can be applied to the preparation of anti-inflammatory drugs.

This invention discloses the application of *Meconopsis spp.* extract in the preparation of anti-Alzheimer's drugs, relating to the field of pharmaceutical preparation technology. The process involves taking dried whole *Meconopsis spp.* herb powder, soaking it in a 0.5% hydrochloric acid-70% ethanol solution for 24 hours, then percolating to extract the extract. The percolate is collected, concentrated under reduced pressure until no alcohol odor remains, diluted with pure water, and then successively defatted with petroleum ether, extracted with ethyl acetate for polyphenols, and extracted with water-saturated n-butanol for alkaloids. The n-butanol layer and the aqueous layer are collected and dried under reduced pressure to obtain the *Meconopsis spp.* extract. The *Meconopsis spp.* extract of this invention can simultaneously act on two core pathological aspects of Alzheimer's disease: on the one hand, it significantly inhibits fibrotic aggregation (inhibition rate up to 61.3%), preventing the formation of neurotoxic plaques; on the other hand, it effectively inhibits microglia-mediated neuroinflammatory responses (NO production inhibition rate up to 74.5%), reducing the damage of the inflammatory microenvironment to neurons.

The application provides a kind of pharmaceutical composition and its preparation method and application, and the active ingredient of pharmaceutical composition is composed of camptothecin, 9-methoxy camptothecin and camptothecin glucoside, and the preparation method comprises: the plant part of Maybium ferrugineum is cleaned, and the pretreated raw material is obtained by drying to change mature and crushing at the first preset temperature;The pretreated raw material is extracted by reflux extraction with the first extraction solvent for the first extraction times, and each extraction is carried out for the first preset time, and the extract obtained by each extraction is combined and concentrated to obtain extract;The extract is dispersed with water, sequentially extracted with petroleum ether, ethyl acetate, and water-saturated n-butanol is extracted to obtain total glycoside;Total glycoside is purified to collect the fraction containing camptothecin, 9-methoxy camptothecin and camptothecin glucoside, and concentrated and crystallized to obtain the pharmaceutical composition.The pharmaceutical composition of the application has significant synergistic antitumor effect, and provides an efficient and low-toxicity treatment option for clinical use.

This invention discloses a thin-layer chromatography (TLC) identification method for five medicinal herbs in Lingqiao Jiedu Pills, belonging to the field of traditional Chinese medicine quality control technology. This invention uses only one sample. First, an ethyl acetate extract is obtained through volatile oil extraction. Petroleum ether (30-60℃)-ethyl acetate is used as the developing solvent, and anisaldehyde-sulfuric acid-ethanol solution is used for color development, simultaneously identifying Schizonepeta tenuifolia and Mentha haplocalyx. Then, the aqueous solution after volatile oil extraction is centrifuged, extracted with ethyl acetate, evaporated to dryness, and dissolved in methanol. Using chloroform-methanol-glacial acetic acid, ethyl acetate-formic acid-glacial acetic acid-water, and dichloromethane-diethyl ether-methanol-n-hexane systems as developing solvents, Forsythia suspensa, Glycyrrhiza uralensis, and Arctium lappa are identified sequentially. This method is simple, rapid, and efficient, producing clear spots without tailing or cross-interference. It exhibits good specificity and reproducibility, making it suitable for the quality control of Lingqiao Jiedu Pills.

The application discloses a preparation method of a triptolide sesquiterpene alkaloid purity standard sample, and belongs to the technical field of natural product separation and purification and standard substance preparation. The method comprises the following steps: obtaining total alkaloid crude products through ethanol extraction and acid-dissolution alkalinization extraction, enriching the total alkaloid crude products through methanol grading, pre-separating the total alkaloid crude products through a specific two-phase system of petroleum ether (60-90 DEG C)-ethyl acetate-isopropyl alcohol-water HSCCC, and finally obtaining monomer products with purity greater than or equal to 99.0% through reverse-phase preparation HPLC refining and low-temperature multiple recrystallization. The obtained standard sample has water content less than or equal to 0.5%, residual solvents meet the requirements of ICH Q3C and the Chinese Pharmacopoeia, and the impurity spectrum fingerprint similarity is greater than or equal to 0.98 under different raw material batches and amplification conditions, so that the batch consistency, traceability and storage stability are significantly improved, and the strict requirements of standard substances in quality control and analytical detection are met.

The application discloses a novel diterpenoid compound with anti-inflammatory activity separated from Origanum vulgare Linn. 20 H 18 O4, and a structural formula is as shown in formula I. A preparation method of the compound comprises the following steps: crushing dried medicinal materials of Origanum vulgare Linn., extracting by using ethanol, concentrating under reduced pressure to obtain total extract; after extraction by using petroleum ether, the target compound is obtained through silica gel column chromatography, Sephadex LH-20 gel column chromatography and preparation type high performance liquid phase chromatography separation and purification. In vitro anti-inflammatory experiments show that the compound can significantly inhibit NO generation in a LPS-induced RAW264.7 macrophage model, and the inhibition rate reaches 62.97%, and the compound shows good anti-inflammatory activity. The compound can be used for preparing medicines for preventing or treating inflammatory diseases such as enteritis, hepatitis and meningitis.

The application relates to the technical field of vanadium battery production, and discloses a vanadium electrolyte for energy storage batteries, a preparation method and application thereof, the vanadium electrolyte comprises vanadium ions, a supporting electrolyte and an additive, the molar ratio of V(III) ions and V(IV) ions is 0.9-1.1:1, the additive is a heterocyclic organic carboxylic acid, the molar ratio of the additive and the vanadium ions is 0.01-2:1, the vanadium ion concentration in the vanadium electrolyte is greater than or equal to 2 mol / L, the sodium ion concentration is less than or equal to 20 mg / L, and the potassium ion concentration is less than or equal to 30 mg / L. The preparation method of the vanadium electrolyte comprises reduction, extraction, reverse extraction and purification, an extraction system of organic phosphoric acid, organic amide and petroleum ether is used, so that the vanadium electrolyte with higher vanadium ion concentration and lower impurity ion concentration such as sodium and potassium can be prepared, and the Coulomb efficiency and voltage efficiency of the energy storage battery can be improved.

This invention discloses an alkaloid, its preparation method, and its application in the preparation of human carboxylesterase 2 inhibitors. The residue of Paeonia lactiflora after ultrasonic extraction was extracted with 95% ethanol and then with ethyl acetate to obtain ethyl acetate extract CS-5. Separation was performed by silica gel column chromatography, using a gradient elution with petroleum ether-acetone mixed solvent and dichloromethane-methanol mixed solvent to obtain six eluent fractions, named A-F. Compound D was separated by silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative HPLC to obtain compound I-2. Compound F was separated by Sephadex LH-20 column chromatography and semi-preparative HPLC to obtain compounds I-1 and I-3. Compound I-1 was separated by a chiral HPLC column to obtain enantiomers I-(+)-1 and I-(–)-1. The four alkaloids of this invention have a pH range of 13.65–14.16. m At concentrations of M, it can significantly inhibit the activity of human carboxylesterase 2. Therefore, it can be applied to the preparation of attenuated and protective agents for anticancer drugs such as irinotecan.

The application discloses a kind of β-amyrin extraction methods, including wood pod red bean, the leaf of wood pod red bean β-amyrin content is 0.18~0.31%, extract new method, including the following steps: step one, dry screening;Step two, extraction mixing;Step three, concentrated extract;Step four, impurity purification;Step five, crystallization precipitation, select wood pod red bean leaf as preparation raw material, obtain simple, reduce raw material cost, prepare β-amyrin extract by multiple extraction mixing in preparation process, fully extract β-amyrin in raw material, reduce the loss of β-amyrin in extraction process, improve yield;Different solvent ratio petroleum ether-acetic ether is used in impurity purification and is eluted twice respectively, remove impurities, improve the purity after preparation, extraction method is simple and easy to operate, reduce production difficulty, save production cost.

The application discloses sea cucumber oil and a preparation method and application thereof. The sea cucumber oil is prepared by using sea cucumber intestines as raw materials, and by using 95% ethanol and petroleum ether to soak and extract the sea cucumber oil, and then by using vacuum distillation. By constructing an acute skin ultraviolet radiation damage mouse model, the application verifies for the first time that the sea cucumber oil can effectively relieve the skin redness and keratin layer increase phenomenon caused by ultraviolet radiation, inhibit the ROS rise, reduce the degree of lipid peroxidation of the organism, improve the loss of collagen in the body, regulate the expression of inflammatory factors in the body, up-regulate the expression of the skin Nrf2 gene and the downstream antioxidant gene NQO1, reduce the expression of the negative feedback factor KEAP1, and play an antioxidant role. The sea cucumber oil has high safety, comprehensively and effectively utilizes the waste resources generated in the sea cucumber processing process, and has a good prospect in the cosmetics and medicines for repairing the acute skin light damage after ultraviolet radiation.

The application discloses a new compound separated from Dendrobium nobile and a preparation method and application thereof, and belongs to the technical field of natural medicines. The preparation method comprises the following steps: step one, drying stems of Dendrobium nobile, crushing, extracting with 95% ethanol by heating and refluxing at least two times, each time for at least 1.5 hours, combining the extract after filtration, and concentrating the extract into thick extract under reduced pressure to obtain a crude extract; step two, dispersing the crude extract with hot water, and sequentially extracting with petroleum ether and dichloromethane; concentrating the water phase under reduced pressure after the extraction to obtain a concentrated solution; step three, loading the concentrated water solution on a macroporous resin column (filler is AB-8 or D101), and gradient eluting with a methanol-water solvent system; and step four, isocratic eluting with 30% acetonitrile-water solvent through preparative liquid phase. The new sesquiterpene glycoside compound is separated from Dendrobium nobile, the new compound has good anti-neuroblastoma activity, and lays a foundation for development and utilization of Dendrobium resources.

This invention discloses a wide-range, high-sensitivity flexible strain sensor based on a grating structure Ecoflex / graphene and its fabrication method, belonging to the field of flexible electronic device technology. The sensor uses a grating structure Ecoflex elastomer modified by swelling with petroleum ether as a substrate, and graphene as a conductive functional layer, fabricated through substrate modification and conductive layer composite processes. This invention utilizes the swelling modification effect of petroleum ether on Ecoflex to optimize the substrate's flexibility and deformation adaptability, combined with the deformation amplification effect of the grating structure, to achieve precise adaptation between substrate deformation and the resistance response of the graphene conductive layer. The sensor fabricated by this invention simultaneously possesses a wide strain detection range of 0-500%, high sensitivity, a response time of no more than 140 ms, and excellent cyclic stability, solving the technical problem of traditional flexible strain sensors' difficulty in simultaneously achieving sensitivity and strain range. It can be applied in fields such as flexible wearables, electronic skin, and intelligent robots.

The application provides a regeneration method of chromatographic packing for separating and purifying fibroin and a regeneration liquid thereof, and the regeneration method of chromatographic packing for separating and purifying fibroin comprises the following steps: taking the chromatographic packing for separating and purifying fibroin, first washing with ethanol slurry, and then washing with petroleum ether slurry to obtain pretreated chromatographic packing; taking the obtained pretreated chromatographic packing, first washing with the regeneration liquid, and then washing with water, and drying to obtain the chromatographic packing for separating and purifying fibroin. The regeneration method of chromatographic packing for separating and purifying fibroin can effectively remove residues in the chromatographic packing for separating and purifying fibroin, maintain the performance of the chromatographic packing, and avoid the residues from polluting subsequent target products.

This invention discloses an acetamine-type sesquiterpene compound, its preparation method, and its applications, belonging to the field of natural compound extraction technology. The preparation method involves extracting dried *Elsholtzia ciliata* by reflux with 95% ethanol, followed by petroleum ether extraction to obtain an extract. The extract can be obtained by column chromatography and semi-preparative high-performance liquid chromatography. The method is simple, time-saving, labor-saving, and yields higher results. Furthermore, the obtained acetamine-type sesquiterpene compound exhibits high anti-neuritis activity, showing significant application potential for developing new drugs to treat neuritis-related diseases.