343 results about "Petroleum ether" patented technology

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention relates to the technical field of biological medicines, in particular to a polycyclic polyisopentenyl cyclopentanone compound as well as a preparation method and application thereof. The polycyclic polyisopentenyl cyclopentanone compound provided by the invention has a structural formula as shown in a formula 1; the preparation method comprises the following steps: by taking dry fruits of hypericum berry as raw materials, carrying out percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and extracting and concentrating the suspension with petroleum ether to obtain a crude extract; the crude extract is taken and subjected to positive and negative phase silica gel column separation, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatographic separation, high performance liquid chromatography preparation and CHIRALPAK IG chiral column resolution, and the polycyclic polyisopentenyl cyclopentanone compound is obtained. The protection activity of the compound on AC16 myocardial cell injury induced by cold ischemia is evaluated, and the compound can be used for preparing the polycyclic polyisopentenyl cyclopentanone compound. The compounds can be used as lead compounds for developing medicines for preventing and treating myocardial cold ischemia injury.

The invention relates to the technical field of natural medicines, in particular to a 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound as well as a preparation method and application thereof. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound disclosed by the invention has a structure as shown in a formula I; the method comprises the following steps: extracting hypericum monogynum by using ethanol and petroleum ether, sequentially eluting the obtained petroleum ether extract through silica gel column chromatography, MCI resin column chromatography, Sephadex LH-20 gel column chromatography and ODS medium-pressure column chromatography, and purifying the collected eluate through preparative ODS liquid chromatography and semi-preparative ODS liquid chromatography to obtain the 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound is extracted from hypericum monogynum for the first time, and the compound has great development value as a novel medicine for preventing or treating calcified aortic valve diseases.

The invention discloses a thin-layer chromatography identification method for cinnamon and ginger in vitality-preserving granules, and belongs to the technical field of pharmaceutical analysis. Aiming at the problems of step-by-step detection, serious cinnamic acid tailing and dependence on toxic solvents in the existing method, the method comprises the following steps: dissolving vitality-preserving particles in water, carrying out ultrasonic treatment, extracting filtrate by using absolute ether, merging extract liquor, evaporating to dryness, and dissolving by adding ethyl acetate to obtain a test solution; preparing a cinnamon reference medicinal material solution and a cinnamic acid and 6-gingerol reference substance solution; a silica gel GF254 thin-layer plate is adopted, petroleum ether-normal hexane-ethyl formate-formic acid is used as a developing solvent for developing, and inspection is conducted under the ultraviolet light of 254 nm and the ultraviolet light of 365 nm respectively. Cinnamon and ginger can be synchronously identified, spots are clear and free of trailing, no toxic solvent exists, durability is high, cost is low, and an accurate and reliable basis can be provided for quality control of the vitality-preserving granules.

The invention discloses a preparation method of an astragalus extract with a uric acid reducing effect, and belongs to the technical field of biological medicines. The method comprises the following steps: concentrating an ethanol extract of radix astragali, extracting by using n-butyl alcohol, ethyl acetate and petroleum ether respectively, and concentrating an extract to obtain n-butyl alcohol, ethyl acetate and petroleum ether extraction parts and residual parts respectively, so as to obtain an n-butyl alcohol extract, namely the radix astragali extract for reducing uric acid. Efficacy experiments show that the normal butanol extraction part of the ethanol extract of the astragalus membranaceus can obviously reduce the serum uric acid level (SUA) of a mouse with hyperuricemia, inhibit the activity of xanthine oxidase (XOD) and effectively improve kidney injury caused by the hyperuricemia. The uric acid reducing effect of the radix astragali n-butyl alcohol extract is close to that of a clinical drug allopurinol, and the radix astragali n-butyl alcohol extract has wide development and application prospects in the aspect of relieving hyperuricemia.

The invention relates to a preparation method of a hydrolysis modified organic silicon high-temperature-resistant anticorrosive coating, and belongs to the technical field of anticorrosive coatings. The preparation method comprises the following steps: heating 1, 1 '-bis (dimethylsilyl) ferrocene, methylbenzene, chloroplatinic acid, 2, 2, 3, 3, 4, 4, 4-heptafluorobutyl acrylate and bis-(2-methylallyl) cyclooctyl-1, 5-diene ruthenium, and reacting to obtain an intermediate product 1; weighing methyltrimethoxysilane, phenyltrimethoxysilane and the intermediate product 1, uniformly stirring and mixing, slowly dropwise adding diluted hydrochloric acid, hydrolyzing, and heating to react, so as to obtain modified silicon resin; adding the modified silicon resin into petroleum ether, and adding silicone oil, an organic tin catalyst, a bis-ammonia coupling agent, filler and a curing agent to obtain the anticorrosive paint.

The invention relates to a continuous production method of ultra-pure 2, 3, 4, 4 '-tetrahydroxybenzophenone, which comprises the following steps: completely dissolving p-hydroxybenzoic acid in high-boiling range petroleum ether, and completely dissolving pyrogallic acid in the high-boiling range petroleum ether; the preparation method comprises the following steps: adding a p-hydroxybenzoic acid solution and a pyrogallic acid solution into an ultra-pure continuous reactor group, adding boron trifluoride diethyl etherate into the ultra-pure continuous reactor group at a flowing speed, reacting at 110-140 DEG C, adding the obtained reaction solution into the next ultra-pure continuous reactor group, stirring in ice water, filtering, washing and recrystallizing to obtain the 2, 3, 4, 5-tetramethyl-3, 5-dihydroxybenzoic acid. According to the present invention, the reaction time is substantially shortened, the conversion rate is 70-80%, the purity of the obtained electronic product is more than or equal to 99.2%, the content of the metal ions such as sodium, aluminum, copper, iron, calcium, zinc and boron is less than or equal to 60 ppb, the reaction materials, the reaction temperature and the reaction rate are controllable, the reaction is sufficient, the yield of the obtained target product is stable, and the method can be used for continuous production.

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

The invention belongs to the field of biological medicine, and particularly relates to an anti-inflammatory and anti-helicobacter pylori compound separated from agilawood and a preparation method thereof. The method comprises the following steps: by taking the agilawood corasta as a raw material, carrying out reflux extraction with ethanol, extracting with petroleum ether to remove volatile components, separating and purifying by adopting various chromatographic technologies such as silica gel column chromatography, reversed-phase column chromatography and semi-preparative HPLC (High Performance Liquid Chromatography) to obtain a monomeric compound, and identifying the structure of the monomeric compound by adopting spectroscopy methods such as ultraviolet, high-resolution mass spectrometry and nuclear magnetic resonance. The structure of a compound 1 is identified as a new 2-(2-phenethyl) chromone dimer, and the compound 1 is named as aquicrasone V. The invention further discloses a preparation method of the compound 1. A biological activity test verifies that the compound has relatively strong anti-inflammatory activity and anti-helicobacter pylori activity.

The application discloses a kind of efficient natamycin purification methods, comprising the following steps: taking two kinds of dichloromethane, chloroform, acetone, butanone, methanol, ethanol, petroleum ether, n-hexane are mixed to obtain mixed solution, adjust the pH of mixed solution to 6.0-7.0, then natamycin crude product is added to mixed solution and stirred into slurry;Slurry is extracted to obtain that natamycin wet product, and the natamycin wet product after extraction is washed with organic solvent;Natamycin wet product after washing is dried, and natamycin purification product is obtained.Compared with prior art, the present application optimizes the existing natamycin purification process, simplifies the natamycin purification method, reduces the production cost, improves the production efficiency, the product purity can reach more than 98%, and the product yield can reach more than 95%.The application has the advantages of less equipment investment, easy operation, less environmental pollution, simple operation, and is suitable for product industrial production.

The application provides a regeneration method of halogenated nitrobenzene continuous hydrogenation catalyst, and relates to the technical field of fixed bed halogenated nitrobenzene hydrogenation. The regeneration method mainly comprises the following steps: using alcohol, alkali, petroleum ether and ethyl acetate to preliminarily clean the bed layer, removing azo compounds and other pollutants on the surface of the catalyst by using the special properties of ionic liquids, and then completing the regeneration of the catalyst through the steps of pickling, precious metal solution treatment and drying. The application overcomes the defects of the prior art, has the advantages of simple operation, mild conditions and low cost, realizes the in-situ removal of surface impurities such as reaction intermediates and azo compounds on the catalyst surface without damaging the structure of the catalyst and disassembling the catalyst, further supplements the lost metal active components, and can effectively restore the catalyst activity.

The application discloses a kind of extraction and purification preparation methods of green brick tea polysaccharide, is related to green brick tea polysaccharide extraction process technical field, specific method is: take green brick tea powder, with petroleum ether, anhydrous ethanol / ethanol solution is extracted in turn, and green brick tea coarse powder is obtained;The green brick tea coarse powder is first extracted with water, then is alcohol precipitated with ethanol, and the precipitate is dissolved with water after centrifugation, and green brick tea polysaccharide crude extract is obtained;Polyamide powder is activated, and polyamide equilibrium column is prepared;Green brick tea polysaccharide crude extract is passed through polyamide equilibrium column, and eluate is collected, concentrated, dialyzed with water, and green brick tea polysaccharide finished product is obtained after drying.The extraction and purification preparation method of the green brick tea polysaccharide is simple, reduces cost, uses less chemical reagent using water extraction alcohol precipitation method, and has lower toxicity, and the green brick tea refined polysaccharide prepared by the method has higher purity and better biological activity.

The application discloses a hot air pretreatment method for extracting oat oil and belongs to the technical field of oil extraction. The application solves the technical problems of low extraction efficiency, easy oxidation of oil and large solvent consumption existing in the prior art. The method comprises the following steps: a. oat bran is dried by hot air at 130-150 DEG C for 15-20 min; b. the oat bran is placed in a negative pressure environment, and ethanol vapor flash treatment is performed for 5-15 min; c. the oat bran is mixed with a mixed solvent of petroleum ether and ethanol, immobilized lipase and natural antioxidant are added, and extraction is performed for 8-12 h, and then filtration separation is performed; d. the first residue is mixed with the mixed solvent, extraction is performed for 4-6 h, and then filtration separation is performed; e. the first supernatant and the second supernatant are combined, the solvent is recovered by reduced pressure distillation, and oat crude oil is obtained; and f. the immobilized lipase is recovered. The method is mainly used for efficiently extracting high-quality oat oil from oat bran.

The invention provides a synthetic method of 2-(2-amino-5-bromo-benzoyl) pyridine, and belongs to the technical field of medicine synthesis. A one-pot boiling method is adopted, 2-cyanopyridine and 4-bromaniline are used as raw materials, BCl3 is used as a catalyst, dichloromethane is used as a solvent, a heat preservation reaction is performed for 5-25 hours at the temperature of-10 DEG C to 40 DEG C, and the product 2-(2-amino-5-bromo-benzoyl) pyridine is obtained through ethyl acetate extraction, anhydrous sodium sulfate drying and dichloromethane and petroleum ether recrystallization. According to the preparation method of the 2-(2-amino-5-bromo-benzoyl) pyridine, provided by the invention, butyl lithium with extremely high dangerousness does not need to be used, the reaction steps are simple, the yield is stable, the treatment is easy, and the yield is high.

The invention provides a basil seed total polyphenol extraction method and a basil seed total polyphenol extract.The extraction method comprises the following steps that a basil seed raw material is subjected to degreasing treatment through a degreasing solvent, and a degreased raw material is prepared; carrying out ultrasonic extraction on the degreased raw material by adopting an ethanol water solution to obtain basil seed total polyphenol; wherein the degreasing solvent comprises petroleum ether; the volume ratio of ethanol in the ethanol aqueous solution is 40-80%; based on g / mL, the material-liquid ratio of the degreased raw material to the ethanol water solution is 1: (20-70); the ultrasonic extraction time is 20 to 70 minutes. According to the extraction method, the extraction efficiency of the basil seed total polyphenol can be improved, and damage to polyphenol activity is reduced.

The application discloses preparation of carbazolyl 3,5-dicyanopyridine derivatives, room temperature phosphor and anti-counterfeiting performance thereof. The preparation method comprises the following steps: adding ortho-, meta- or para-carbazolyl benzaldehyde and malononitrile into a reaction bottle, stirring and reacting under room temperature conditions with sodium hydroxide as a catalyst and anhydrous methanol as a solvent, and obtaining ortho-, meta- or para-carbazolyl 3,5-dicyanopyridine by separating and purifying the reaction crude product. Crystals of the three compounds are obtained by a solvent diffusion method with dichloromethane-petroleum ether as a solvent, and the crystal state room temperature phosphor material is obtained. The crystals are doped with urea / triphenylphosphine at a weight ratio of 1:100, the doped crystals are uniformly ground and mixed, and then heated to be molten, so that the host-guest doped phosphor material is obtained, and the novel material is used in the field of anti-counterfeiting patterns.

The invention relates to the technical field of agriculture, and provides germacane type sesquiterpenoids in tithonia diversifolia as well as a preparation method and application thereof.The preparation method comprises the steps that stem and leaf parts of tithonia diversifolia are subjected to ethanol cold-soaking extraction, and an extracting solution is subjected to vacuum concentration to obtain a total extract; suspending the total extract with water, sequentially extracting with petroleum ether and ethyl acetate, and concentrating the extract liquor under reduced pressure to obtain each extraction layer; carrying out silica gel column chromatography separation on the ethyl acetate extraction layer, and combining to obtain six parts A-F; carrying out MCI column chromatography separation on the part D, and combining to obtain thirteen parts D1-D13; carrying out silica gel column chromatography separation on the D9 part, and combining to obtain fifteen parts D91-D915; and separating the D915 part by adopting HPLC (High Performance Liquid Chromatography) to prepare two germacane type sesquiterpenoids. The preparation method disclosed by the invention is simple and good in reproducibility, and the prepared germacane type sesquiterpenoids are high in purity and have specific herbicidal activity on foreign invasive plants such as ambrosia trifida and ambrosia esculenta.

The application belongs to the technical field of all-solid-state lithium batteries, and provides a hydrophobic sulfide electrolyte film and a preparation method and application thereof. The method comprises the following steps: mixing polyvinylidene fluoride-hexafluoropropylene, a lithium salt and a solvent to react under a protective atmosphere to obtain a lithium salt polymer binder; mixing the sulfide electrolyte, the lithium salt polymer binder and the solvent, and sequentially coating and heat treating to obtain a sulfide electrolyte film under a protective atmosphere; mixing 1H, 1H, 2H, 2H-perfluorodecyltriethoxysilane, tetraethyl silicate, germanium aluminum lithium phosphate, water, ethanol and ammonia water to react to obtain hydrophobic particles; and spraying a petroleum ether solution of the hydrophobic particles on the surface of the sulfide electrolyte film to obtain the hydrophobic sulfide electrolyte film. The hydrophobic sulfide electrolyte film prepared by the application has excellent ion conductivity, mechanical properties, air stability and hydrophobicity.

The invention belongs to the technical field of medicine research, and particularly relates to a gentiana macrophylla extract as well as a preparation method and application thereof. The preparation method of the gentiana macrophylla extract comprises the following steps: by taking gentiana macrophylla as a raw material, adding an ethanol solution with the mass fraction of 70-80%, carrying out heating reflux extraction, collecting an extracting solution, and concentrating to obtain a crude gentiana macrophylla extract; taking an aqueous solution of the gentiana macrophylla crude extract, extracting by taking petroleum ether as an extracting agent, reserving water layer liquid, and drying to obtain a water layer sample; the water layer sample is eluted through macroporous adsorption resin, pure water and an ethanol solution with the mass fraction being 35%-40% are sequentially used as eluents, ethanol eluent is collected and dried, and the water layer sample is obtained. Tests prove that the gentiana macrophylla extract can improve the MASH damp-heat accumulation symptom and liver functions, inhibit the increase of liver proinflammatory cytokines, and relieve MASH hepatic lobule cell fat accumulation, foam and balloon-like degeneration, cell nucleus atrophy and inflammatory lesions.

The invention discloses a compound with remarkable 5-hydroxytryptamine (5-HT) reuptake inhibition activity, a preparation method of the compound and application of the compound in preparation of anti-depression drugs. And the compound is a compound Oreonudine C. The invention further discloses a preparation method of the compound. The preparation method of the Oreonudine C comprises the following steps: extracting with 95% ethanol, acidifying the extract, extracting with petroleum ether, adjusting the acid water layer to be alkaline with alkali liquor, extracting with chloroform, and performing silica gel column chromatography, C18 ODS column chromatography and preparative high performance liquid chromatography on the chloroform extraction part to obtain the Oreonudine C. The invention further discloses a preparation method of the Oreonudine C. The preparation method of the Oreonudine C comprises the following steps: extracting with 95% ethanol, acidifying the extract, extracting with petroleum ether, adjusting the acid water layer to be alkaline with alkali liquor, and obtaining the Oreonudine C. In-vitro experiments show that the compound Oreonudine C remarkably inhibits reabsorption of 5-HT (the inhibition rate is 60.4%) in mouse hippocampal neuronal cells HT22, and the inhibition rate of the compound Oreonudine C is superior to that of a positive control drug amitriptyline (the inhibition rate is 53.6%). The invention provides an important candidate compound for developing efficient and novel antidepressant drugs.

The invention relates to the technical field of sesamol extraction, in particular to a sesamol extraction process for food preservation, which comprises the following steps: step 1, raw material pretreatment: pretreating prepared sesame raw materials, selecting qualified sesame raw materials, removing impurities, rinsing the sesame raw materials with clear water, and draining the sesame raw materials for later use; step 2, carrying out drying treatment on the drained sesames; and step 3, squeezing and degreasing the dried sesame to obtain sesame cake meal and sesame oil. Microwave pretreatment is adopted to replace traditional baking, more polyphenol active ingredients are reserved, dried sesame is squeezed and degreased, purified sesame oil serves as a raw material and is soaked in organic solvents such as ethyl alcohol and petroleum ether, a soaked mixed solution is separated and purified, the mixed solution is subjected to reduced pressure distillation and concentration, an extracting solution is obtained, and the finished product is obtained. And carrying out freeze drying or vacuum drying to obtain a crude extract, and recrystallizing the crude extract with petroleum ether or cyclohexane to obtain the high-purity sesamol, so that the purity of the sesamol obtained by the method is high.

The application discloses a pterosin sesquiterpenoid compound and a preparation method and application thereof, belongs to the technical field of biological medicines, aims at the problems of a research blank of pterosin sesquiterpenoid compounds in Anemone raddeana and a lack of structure-confirmed anti-inflammatory active monomers, and provides a pterosin sesquiterpenoid compound with a structural formula as shown in the following.The compound preparation needs to first crush dried whole grass of Anemone raddeana, extract by heating and refluxing with an organic solution, concentrate under reduced pressure to obtain total extract; then, the extract is extracted by petroleum ether, ethyl acetate and n-butanol step by step, the ethyl acetate part extract is enriched, and the high-purity product is prepared through multi-step purification of a silica gel column, a C-18 reverse phase column, a gel column and semi-preparative liquid chromatography.In addition, the compound can dose-dependently inhibit the NO release of LPS-induced macrophages, and can be used for preparing anti-inflammatory drugs for treating or preventing inflammatory diseases related to excessive production of nitric oxide, fills the research blank, and provides a high-quality candidate component for anti-inflammatory drug research and development.

The present application relates to a kind of two-edged needle medicinal materials and its preparation fast identification method, the preparation is containing two-edged needle Chinese medicine preparation such as Huatou Fengtongbao tablet, Huatou Fengtongbao capsule, two-edged needle analgesic tablet, the method includes the respective preparation of each test solution of the sample, then each test solution is spotted on the same silica gel G thin layer plate, using the uplink development method, with volume ratio 12:1:4:0.1 of petroleum ether-cyclohexane-ethyl acetate-glacial acetic acid as developing agent, development, take out, natural cool dry, under the inspection of ultraviolet light, in the chromatogram of test sample, in the corresponding position with the chromatogram of control drug material, same color fluorescent spot is shown.The present application can be used for the fast identification of two-edged needle medicinal materials and related preparation, and compared with the existing identification technology of two-edged needle and its preparation, no toxic organic solvent is used, with the advantages of simple, fast, environmental protection, green, result exclusive, accurate, controllable operation method.

The application discloses a preparation method of high-purity trans-4-propionylcyclohexylbenzene, and belongs to the technical field of liquid crystal material preparation. The method is based on dynamic synergy of three-stage gradient temperature control, seed addition in batches and intermittent stirring, and in a single petroleum ether solvent system, crystal growth is induced through ultralow-temperature cyclic crystallization and precise temperature control; in combination with a matching filter-drying integrated machine, continuous operation of low-temperature solid-liquid separation and vacuum drying is realized. The application significantly improves the purity and yield stability of the product, and one-time high-purity crystals are obtained, overcoming the problems of incomplete impurity separation, high energy consumption and large batch fluctuation in the traditional process, and simultaneously adapting to the industrial production demand, and providing a green and efficient high-purity preparation path for liquid crystal intermediates.

The present application relates to a surfactant composition containing an alkyl hydroxy ether acetate (A) represented by the following general formula (1) and a compound (I) detected between retention time 10 minutes and 17 minutes in a gas chromatography using petroleum ether in conformity with JIS K8593, the ratio of the peak area of the compound (I) to the peak area of the petroleum ether determined by the gas chromatography being 0.02% to 50%.R 1 -CH(OH)-CH2-OCH2COOM 1 (1) [In the general formula (1), R 1 represents an alkyl group having 8 to 12 carbon atoms, M 1 represents a hydrogen atom, a sodium atom, a potassium atom or triethanolammonium.]

Cell parameters of the crystal compound are as follows: diffraction data are collected in an omega-theta scanning mode by using MoK alpha rays monochromatized by a graphite monochromator on an Oxford X-ray single crystal diffractometer at the temperature of 273 k, and the crystal compound is characterized in that the crystal belongs to a triclinic system, P-1; the cell parameters are as follows: alpha is equal to 79.857 (3) degrees; beta is equal to 87.082 (3) degrees; gamma is equal to 87.829 (3) degrees; the synthesis method of the crystal chlorine compound (I) comprises the following steps: weighing 0.02 35 g of benzophenone hydrazone and 0.6720 g of 1.0 g of 7-chloroisatin and acetic acid ketone monohydrate complex, putting into a 150mL flask, adding 100.0 mL of chlorobenzene as a solvent, stirring at room temperature for 48 hours, carrying out column chromatography separation, eluting with petroleum ether / dichloromethane (1 / 1), and carrying out vacuum drying to obtain the crystal chlorine compound (I). And naturally volatilizing the collected front component points to obtain the target crystal compound I, namely the 6-bromo-3-diphenylmethylene indoline-2-ketone crystal. The application of the crystal chlorine compound (I) is that the crystal chlorine compound (I) has a relatively strong inhibition effect on epidermophyton flocculent, and the IC50 value of the crystal chlorine compound (I) is 0.063 + / -0.008.

This invention relates to the field of rose separation and purification technology, specifically a novel benzofuran compound from rose petals, its extraction method, and its application. The method involves extracting the total rose extract obtained by heating and reflux of dried rose petals, then extracting the petroleum ether fraction. After elution and separation, the third fraction is further eluted with silica gel, the second fraction is eluted again, and the third fraction is purified by elution. The novel benzofuran compound from rose petals is obtained at 21.0 minutes. This invention discloses for the first time the extraction and isolation of a benzofuran compound from rose petals, and its antibacterial pharmacodynamic effects were tested. The experiments clearly showed that the compound has a strong inhibitory effect on Staphylococcus aureus and Escherichia coli, thus enabling its application in the preparation of antibacterial drugs and antibacterial health products.

The invention discloses a wax recovery treatment method which comprises the following steps: stirring, dissolving and precipitating wax in a sodium hydroxide solution to obtain supernate; adding the supernatant into boric acid, stirring to obtain jelly, filtering the jelly, washing, and drying to obtain a dried substance; mixing and stirring the dried substance and methanol, and drying to obtain a wax solid substance; and purifying the wax solid with a distillation extract of petroleum ether. According to the method, impurities in the wax are effectively removed, and the recovery purity and quality of the wax are improved, so that the service life of the wax is prolonged, the production cost is reduced, and the aims of saving resources and protecting the environment are fulfilled.

The invention discloses hump conjugated linoleic acid, a hump conjugated linoleic acid microcapsule and a preparation method and application of the hump conjugated linoleic acid microcapsule, and belongs to the technical field of deep processing of hump fat. According to the method, petroleum ether is used as a solvent, crude fat is extracted from hump fat through a Soxhlet extraction method, NaOH-ethanol is used for saponifying the crude fat, then a low-temperature crystallization method is combined with a urea adduction method, and finally, the conjugated linoleic acid is effectively separated and purified. The determination of the free radical scavenging ability of DPPH and ABTS shows that the obtained CLA has oxidation resistance. Extracted and purified conjugated linoleic acid is used as a core material, whey protein isolate and maltodextrin are selected as wall materials, an emulsion is prepared, and microcapsule powder is prepared by a direct vacuum freeze-drying method. Experiments show that the prepared conjugated linoleic acid microcapsule has a complete structure and good thermal stability.